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1.
J Chromatogr A ; 1688: 463738, 2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36574747

RESUMO

Determination of amphetamine-type drugs (ATSs) in urine and wastewater is a simplified approach for the widespread monitoring of ATSs abuse. To improve the sensitivity of the analytical methods, molecularly imprinted polymers (MIPs) based solid-phase extraction (SPE) pretreatment attracted great attention in this field. Generally, smaller particle sizes and more uniform morphology of the MIPs could provide higher detection sensitivity. Our previous works showed reflux precipitation polymerization (RPP) is a method for synthesizing monodispersed MIPs with small particle size. However, synthesis of uniform spherical MIPs towards a group of targets has never been reported. Therefore, in the present work, MIPs towards a group of ATSs were synthesized via RPP with a pseudo template for the first time. After screening potential pseudo-templates, N-methylphenylethylamine (MPEA) was selected as the optimal pseudo-template. MPEA-MIPs were characterized by scanning electron microscope (SEM), FT-IR spectroscopy and X-ray photoelectron spectroscopy (XPS) spectra. Adsorption isotherms, adsorption kinetics and selectivity were evaluated, and the experimental results indicated that the MPEA-MIPs possessed good selectivity and adsorption property towards ATSs. After optimization of the MIP-SPE procedure, the MIP-SPE cartridges were then coupled with liquid chromatography and tandem mass spectrometry (LC-MS/MS) for determination of ATSs. The evaluation results showed that MIP-SPE-LC-MS/MS displayed good linearity (R2 >0.991) in the linear range (1.0-50.0 µg/L for urine and 0.5-50.0 µg/L for wastewater), and low matrix effect (85-112%). The limit of detection (LOD) was 0.05 -0.29 µg/L, and the accuracy (85-115%) and repeatability (RSD ≤ 15%) were satisfactory at low, medium and high concentrations. To the best of our knowledge, this is the first time that dummy MIPs towards a group of ATSs were synthesized by RPP polymerization, which showed great potential for the detection of ATSs in urine and wastewater.


Assuntos
Estimulantes do Sistema Nervoso Central , Impressão Molecular , Anfetamina , Cromatografia Líquida , Polímeros Molecularmente Impressos , Polimerização , Espectroscopia de Infravermelho com Transformada de Fourier , Polímeros/química , Espectrometria de Massas em Tandem/métodos , Extração em Fase Sólida/métodos , Impressão Molecular/métodos , Adsorção , Cromatografia Líquida de Alta Pressão/métodos
2.
Polymers (Basel) ; 14(23)2022 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-36501707

RESUMO

The hydrophilicity and inherent flammability of cotton textiles severely limit their usage. To solve these drawbacks, a superhydrophobic and flame-retardant (SFR) coating made of chitosan (CH), ammonium polyphosphate (APP), and TiO2-SiO2-HMDS composite was applied to cotton fabric using simple layer-by-layer assembly and dip-coating procedures. First, the fabric was alternately immersed in CH and APP water dispersions, and then immersed in TiO2-SiO2-HMDS composite to form a CH/APP@TiO2-SiO2-HMDS coating on the cotton fabric surface. SEM, EDS, and FTIR were used to analyze the surface morphology, element composition, and functional groups of the cotton fabric, respectively. Vertical burning tests, microscale combustion calorimeter tests, and thermogravimetric analyses were used to evaluate the flammability, combustion behavior, thermal degradation characteristics, and flame-retardant mechanism of this system. When compared to the pristine cotton sample, the deposition of CH and APP enhanced the flame retardancy, residual char, heat release rate, and total heat release of the cotton textiles. The superhydrophobic test results showed that the maximal contact angle of SFR cotton fabric was 153.7°, and possessed excellent superhydrophobicity. Meanwhile, the superhydrophobicity is not lost after 10 laundering cycles or 50 friction cycles. In addition, the UPF value of CH/APP@TiO2-SiO2-HMDS cotton was 825.81, demonstrating excellent UV-shielding properties. Such a durable SFR fabric with a facile fabrication process exhibits potential applications for both oil/water separation and flame retardancy.

3.
Polymers (Basel) ; 14(21)2022 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-36365767

RESUMO

1,2,4 trichlorobenzene (1,2,4-TrCB) is a persistent organic pollutant with chemical stability, biological toxicity, and durability, which has a significant adverse impact on the ecological environment and human health. In order to solve the pollution problem, bagasse cellulose is used as the basic framework and nano TiO2 is used as the photocatalyst to prepare composite carriers with excellent performance. Based on this, an intimate coupling of photocatalysis and biodegradation (ICPB) system combining photocatalysis and microorganisms is constructed. We use the combined technology for the first time to deal with the pollution problem of 1,2,4-TrCB. The biofilm in the composite carrier can decompose the photocatalytic products so that the removal rate of 1,2,4-TrCB is 68.01%, which is 14.81% higher than those of biodegradation or photocatalysis alone, and the mineralization rate is 50.30%, which is 11.50% higher than that of photocatalysis alone. The degradation pathways and mechanisms of 1,2,4-TrCB are explored, which provide a theoretical basis and potential application for the efficient degradation of 1,2,4-TrCB and other refractory organics by the ICPB system.

4.
Water Res ; 225: 119191, 2022 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-36215841

RESUMO

There are numerous studies concerning the impacts of widespread microplastic pollution on the ecological environment, and it shows synergistic effect of microplastics and co-exposed pollutants in risk enhancement. However, the control methods for removing harmful pollutants from microplastic surface to reduce their ecological toxicity has rarely been explored. In this paper, magnetic graphitized biochar as a catalyst is shown to achieve 97% removal of tetrabromobisphenol A (TBBPA) from microplastics by biochar mediated electron transfer. The changes in the surface and structure of microplastics caused by various aging processes affected the pollutant attachment and subsequent removal efficiency. After chlorination, the highest disinfection by-product (DBP) generation potential was observed by the group of microplastics attached with TBBPA. The oxidation system of biochar activating peroxodisulfate (PDS) can not only reduce the kinds of DBPs, but also greatly reduce the total amount of detected DBPs by 76%, as well as reducing the overall toxicity. This paper highlights an overlooked contribution of pollutant attachment to the potential risks of DBP generated from natural microplastics during chlorination process, and provides the underlying insights to guide the design of a biochar-based catalyst from wastes to achieve the removal of TBBPA from microplastics and reduce the risks and hazards of co-contamination.


Assuntos
Microplásticos , Poluentes Químicos da Água , Desinfecção , Plásticos , Poluentes Químicos da Água/análise
5.
Polymers (Basel) ; 14(15)2022 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-35893962

RESUMO

Intimate coupling photocatalysis and biodegradation treatment technology is an emerging technology in the treatment of refractory organic matter, and the carrier plays an important role in this technology. In this paper, sugarcane cellulose was used as the basic skeleton, absorbent cotton was used as a reinforcing agent, anhydrous sodium sulfate was used as a pore-forming agent to prepare a cellulose porous support with good photocatalytic performance, and nano-TiO2 was loaded onto it by a low-temperature bonding method. The results showed that the optimal preparation conditions of cellulose carriers were: cellulose mass fraction 1.0%; absorbent cotton 0.6 g; and Na2SO4 60 g. The SEM, EDS and XPS characterization further indicated that the nano-TiO2 was uniformly loaded onto the cellulose support. The degradation experiments of Rhodamine B showed that the nano-TiO2-loaded composite supports had good photocatalytic performance. The degradation rate of 1,2,4-trichlorobenzene was more than 92% after 6 cycles, and the experiment of adhering a large number of microorganisms on the carriers before and after the reaction showed that the cellulose-based carriers obtained the required photocatalytic performance and stability, which is a good cellulose porous carrier.

6.
Angew Chem Int Ed Engl ; 61(36): e202206894, 2022 09 05.
Artigo em Inglês | MEDLINE | ID: mdl-35789171

RESUMO

Peroxynitrite (ONOO- ) plays a critical role in Alzheimer's disease (AD). To reveal the ONOO- influx in AD brains, an activatable activity-based fluorescence probe Rd-DPA3 was designed by a structure-modulated strategy. Taking advantage of ONOO- -initiated two-step cascade reactions of a novel chemical trigger, Rd-DPA3 specifically responds to ONOO- in 0.3 mM of other reactive oxygen species (ROS) and varied proteins, and gives an intensive fluorescence enhancement (F/F0 =50). Moreover, with its mitochondria-targeting ability, Rd-DPA3 can be used to efficiently monitor the alternations of intracellular ONOO- levels in cerebral cells during oxidative stress. Significantly, due to NIR emission and good blood-brain barrier (BBB) crossing ability, Rd-DPA3 is suitable for in vivo imaging of cerebral ONOO- influx and illustrating an age-dependent accumulation of ONOO- in AD mice brains.


Assuntos
Doença de Alzheimer , Ácido Peroxinitroso , Doença de Alzheimer/diagnóstico por imagem , Animais , Encéfalo/diagnóstico por imagem , Encéfalo/metabolismo , Fluorescência , Corantes Fluorescentes , Camundongos
7.
J Environ Sci (China) ; 122: 50-61, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-35717090

RESUMO

The widespread contamination of water systems with antibiotics and heavy metals has gained much attention. Intimately coupled visible -light-responsive photocatalysis and biodegradation (ICPB) provides a novel approach for removing such mixed pollutants. In ICPB, the photocatalysis products are biodegraded by a protected biofilm, leading to the mineralization of refractory organics. In the present study, the ICPB approach exhibited excellent photocatalytic activity and biodegradation, providing up to ∼1.27 times the degradation rate of sulfamethoxazole (SMX) and 1.16 times the Cr(VI) reduction rate of visible-light-induced photocatalysis . Three-dimensional fluorescence analysis demonstrated the synergistic ICPB effects of photocatalysis and biodegradation for removing SMX and reducing Cr(VI). In addition, the toxicity of the SMX intermediates and Cr(VI) in the ICPB process significantly decreased. The use of MoS2/CoS2 photocatalyst accelerated the separation of electrons and holes, with•O2- and h+ attacking SMX and e- reducing Cr(VI), providing an effective means for enhancing the removal and mineralization of these mixed pollutants via the ICPB technique. The microbial community results demonstrate that bacteria that are conducive to pollutant removal are were enriched by the acclimation and ICPB operation processes, thus significantly improving the performance of the ICPB system.


Assuntos
Poluentes Ambientais , Sulfametoxazol , Biofilmes , Catálise , Cromo , Titânio
8.
J Environ Manage ; 318: 115595, 2022 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-35772268

RESUMO

Intimate coupling of photocatalysis and biodegradation (ICPB) is considered a promising approach for the degradation of recalcitrant organic compounds. In this work, using Trichoderma with benzene degradation ability coupled with activated sludge as a biological source and sugarcane bagasse cellulose composite as a carrier, the ICPB system showed excellent degradation and mineralization of trichlorobenzene under visible light induction. The biofilm inside the ICPB carrier can degrade and mineralize the photocatalytic products. ICPB increased the degradation efficiency of 1,2,3-TCB and 1,3,5-TCB by 12.43% and 4.67%, respectively, compared to photocatalysis alone. The biofilms inside the ICPB carriers can mineralize photocatalytic products, which increases the mineralization efficiency by 18.74%. According to the analysis of intermediates, the degradation of 1,2,3-TCB in this coupled system involved stepwise dechlorination and ring opening. The biofilm in ICPB carrier evolved to be enriched in Cutaneotrichosporon, Trichoderma, Apiotrichum, Zoogloea, Dechloromonas, Flavihumibacter and Cupriavidus, which are known for biodegradable aromatic hydrocarbon and halogenate. Novel microbial seeds supplemented with Trichoderma-based ICPB seem to provide a new potential strategy for effective degradation and mineralization of TCB.


Assuntos
Celulose , Saccharum , Bactérias/metabolismo , Biodegradação Ambiental , Celulose/metabolismo , Clorobenzenos , Titânio
9.
Front Pharmacol ; 13: 888054, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35571101

RESUMO

This study aimed to explore the effect of baicalein on the pharmacokinetics of cilostazol (CLZ) and its two metabolites 3,4-dehydro cilostazol (3,4-CLZ) and 4'-trans-hydroxy cilostazol (4'-CLZ) in rats using a newly established ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method. Ticagrelor was used as an internal standard (IS), then cilostazol and its two metabolites were separated by means of a UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 µm) using gradient elution method with 0.4 ml/min of flow rate. Acetonitrile as organic phase and water with 0.1% formic acid as aqueous phase constructed the mobile phase. Selective reaction monitoring (SRM) mode and positive ion mode were preferentially chosen to detect the analytes. Twelve SD rats were divided into two groups (n = 6) when CLZ was administered orally (10 mg/kg) with or without oral baicalein (80 mg/kg). The selectivity, linearity, recovery, accuracy, precision, matrix effect and stability of UPLC-MS/MS assay were satisfied with the standards of United States Food and Drug Administration guidelines. In control group, AUC0-∞ and Cmax of CLZ were 2,169.5 ± 363.1 ng/ml*h and 258.9 ± 82.6 ng/ml, respectively. The corresponding results were 3,767.6 ± 1,049.8 ng/ml*h and 308.6 ± 87.9 ng/ml for 3, 4-CLZ, 728.8 ± 189.9 ng/ml*h and 100.3 ± 51.3 ng/ml for 4'-CLZ, respectively. After combination with baicalein, AUC0-∞ and Cmax of CLZ were 1.48, 1.38 times higher than the controls. Additionally, AUC0-∞ and Cmax were separately decreased by 36.12 and 19.54% for 3,4-CLZ, 13.11 and 44.37% for 4'-CLZ. Baicalein obviously alters the pharmacokinetic parameters of CLZ, 3,4-CLZ and 4'-CLZ in rats. These results suggested that there was a potential drug-drug interaction between baicalein and CLZ. Therefore, it must raise the awareness when concomitant use of CLZ with baicalein, the dosage regimen of CLZ should be taken into consideration, if this result is confirmed in clinical studies.

10.
Br J Math Stat Psychol ; 75(3): 638-667, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-35578396

RESUMO

Attributes and the Q-matrix are the central components for cognitive diagnostic assessment, and are usually defined by domain experts. However, it is challenging and time consuming for experts to specify the attributes and Q-matrix manually. Thus, there is an urgent need for an automatic and intelligent means to address this concern. This paper presents a new data-driven approach for learning the Q-matrix from response data. By constructing a statistical index and a heuristic algorithm based on Boolean matrix factorization, the response matrix is decomposed into the Boolean product of the Q-matrix and the attribute mastery patterns. The feasibility of the proposed approach is evaluated using simulated data generated under various conditions. A real data example is also presented to demonstrate the usefulness of the proposed approach.


Assuntos
Algoritmos , Cognição
11.
Mol Biol Cell ; 33(8)2022 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-35544303

RESUMO

Normal tissue and organ morphogenesis requires epithelial cell plasticity and conversion to a mesenchymal phenotype through a tightly regulated process-epithelial-to-mesenchymal transition (EMT). Alterations of EMT go far beyond cell-lineage segregation and contribute to pathologic conditions such as cancer. EMT is subject to intersecting control pathways; however, EMT's metabolic mechanism remains poorly understood. Here, we demonstrate that transforming growth factor ß (TGF-ß)-induced EMT is accompanied by decreased fatty acid oxidation (FAO) and reduced acetyl-coenzyme A (acetyl-CoA) levels. Acetyl-CoA is a central metabolite and the sole donor of acetyl groups to acetylate key proteins. Further, the short-chain fatty acid acetate increases acetyl-CoA levels--robustly inhibiting EMT and cancer cell migration. Acetate can restore EMT-associated α-tubulin acetylation levels, increasing microtubule stability. Transcriptome profiling and flow cytometric analysis show that acetate inhibits the global gene expression program associated with EMT and the EMT-associated G1 cell cycle arrest. Taken together, these results demonstrate that acetate is a potent metabolic regulator of EMT and that therapeutic manipulation of acetate metabolism could provide the basis for treating a wide range of EMT-linked pathological conditions, including cancer.


Assuntos
Transição Epitelial-Mesenquimal , Fator de Crescimento Transformador beta , Acetatos/farmacologia , Acetilcoenzima A , Transição Epitelial-Mesenquimal/genética , Ácidos Graxos Voláteis , Fator de Crescimento Transformador beta/farmacologia
12.
Front Cardiovasc Med ; 9: 865036, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35463784

RESUMO

Background: Conflicting findings of the association between serum uric acid (SUA) and atrial fibrillation (AF) have been reported in both men and women. The sex-specific associations between SUA and the risk of AF are unclear, although hyperuricemia is independently associated with the risk of AF. We performed this meta-analysis to assess the sex-specific effect of SUA on the risk of AF. Methods: The PubMed, EMBASE, and Cochrane Library databases were searched up to October 3, 2021, for studies that reported sex-specific associations of SUA levels with AF. Linear relationships were assessed by the generalized least squares trend estimation. This study was registered with PROSPERO (42020193013). Results: Ten eligible studies with 814,804 participants (415,779 men and 399,025 women) were identified. In the category analysis, high SUA was associated with an increased risk of AF in both men (OR: 1.42; 95% CI, 1.18-1.71, I2 = 34%) and women (OR: 2.02; 95% CI, 1.29-3.16, I2 = 70%). In the dose-response analysis, for each 60 µmol/L (1 mg/dL) increase in the SUA level, the risk of AF increased by 15% (OR: 1.15; 95% CI, 1.07-1.25, I2 = 74%) in men and 35% (OR: 1.35; 95% CI, 1.18-1.53, I2 = 73%) in women. There was a borderline difference in the impact of SUA on the risk of AF between men and women (P for interaction = 0.05). A significant linear relationship between SUA and the risk of AF was observed in men (P for non-linearity = 0.91) and women (P for non-linearity = 0.92). Conclusions: This study suggested that there was a significant linear relationship between SUA and the risk of AF among men and women, with a higher risk estimate for women. Additional trials are required to assess the effect of reduced SUA therapy on AF incidence. Systematic Review Registration: https:www.crd.york.ac.uk/PROSPERO/, identifier: CRD 42020193013.

13.
ACS Omega ; 7(14): 12158-12170, 2022 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-35449975

RESUMO

Widespread application of TiO2 for degradation of antibiotics is restricted by mainly the low photodegradation efficiency under solar irradiation. To expand the application of TiO2, the key factors that should be improved are visible-light response, yield of electrons and holes, and durability. Herein, we report a visible-light responsive and durable sugarcane-bagasse-derived biochar supported hydrogenated TiO2 (HSCB/H2-TiO2) photocatalyst with higher electron production fabricated by a facile one-pot hydrogenation. Mild hydrogenation temperature preserved the lotus-stem-like structure of sugarcane bagasse and gave the photocatalyst great separability. The superior durability of HSCB/H2-TiO2 was demonstrated by 12 rounds of repeated degradation of methylene blue (MB). In addition, the electron paramagnetic resonance (EPR) results demonstrated that the biochar skeleton contains abundant persistent free radicals (PFRs), which can provide excess electrons to form more •O2 -. Meanwhile, radical quenching experiment and EPR radical trapping results also revealed that •O2 - was the most dominant species for enrofloxacin (ENR) degradation. Thus, the as-fabricated photocatalyst shows excellent solar-driven degradation of ENR, and 95.6% of ENR was degraded in 180 min under simulated solar irradiation. Possible ENR degradation pathways and mechanism are also proposed based on the identified intermediates.

14.
FASEB J ; 36(4): e22237, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-35226776

RESUMO

Cardiovascular diseases (CVDs), including all types of disorders related to the heart or blood vessels, are the major public health problems and the leading causes of mortality globally. (Pro)renin receptor (PRR), a single transmembrane protein, is present in cardiomyocytes, vascular smooth muscle cells, and endothelial cells. PRR plays an essential role in cardiovascular homeostasis by regulating the renin-angiotensin system and several intracellular signals such as mitogen-activated protein kinase signaling and wnt/ß-catenin signaling in various cardiovascular cells. This review discusses the current evidence for the pathophysiological roles of the cardiac and vascular PRR. Activation of PRR in cardiomyocytes may contribute to myocardial ischemia/reperfusion injury, cardiac hypertrophy, diabetic or alcoholic cardiomyopathy, salt-induced heart damage, and heart failure. Activation of PRR promotes vascular smooth muscle cell proliferation, endothelial cell dysfunction, neovascularization, and the progress of vascular diseases. In addition, phenotypes of animals transgenic for PRR and the hypertensive actions of PRR in the brain and kidney and the soluble PRR are also discussed. Targeting PRR in local tissues may offer benefits for patients with CVDs, including heart injury, atherosclerosis, and hypertension.


Assuntos
Doenças Cardiovasculares/etiologia , Receptores de Superfície Celular/fisiologia , Animais , Cardiomegalia/etiologia , Cardiomiopatias/etiologia , Doenças Cardiovasculares/tratamento farmacológico , Células Endoteliais/fisiologia , Humanos , Hipertensão/etiologia , Músculo Liso Vascular/citologia , Músculo Liso Vascular/fisiologia , Traumatismo por Reperfusão Miocárdica/etiologia , Neovascularização Fisiológica , Receptores de Superfície Celular/antagonistas & inibidores , Sistema Renina-Angiotensina/fisiologia
15.
J Environ Manage ; 302(Pt B): 114111, 2022 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-34800771

RESUMO

In this study, the visible-light-induced intimately coupled photocatalysis and biodegradation (ICPB) technology was fabricated using the TiO2/bagasse cellulose composite as the carrier and Phanerochaete mixed activated sludge as the biological source. The ICPB degradation effect of elemental chlorine free (ECF) bleaching wastewater was evaluated via the response surface design. Then, the wastewater was characterized, including absorbable organic halogen (AOX), dissolved organic carbon (DOC), chemical oxygen demand (COD), chroma, pH, suspended solids, and the organic compound changes in wastewater were analyzed by fourier transform infrared spectroscopy (FT-IR). Under the optimal conditions of pH 7, carrier filling rate of 5%, aeration rate of 2 L/min, and reaction time of 7 h, the degradation efficiencies of AOX, COD, and DOC were 95%, 91%, and 82%, respectively. The X-ray photoelectron spectroscopy (XPS) results of the ICPB carrier after the reaction were almost identical to those before the reaction. The biomass and its activity on the ICPB system were analyzed by the dominant bacteria during degradation (Curaneotrichosporon, Paenibacillus, Cellulonas, Phanerochaete, Dechlorobacter, Rhodotorula, Sphingobacterium, and Ruminiclostridium), which had a good degradation effect on wastewater. This study affords a novel method for the degradation of ECF bleaching wastewater and a new idea for ICPB technology optimization.


Assuntos
Matéria Orgânica Dissolvida , Biodegradação Ambiental , Espectroscopia de Infravermelho com Transformada de Fourier , Titânio
16.
Mikrochim Acta ; 189(1): 15, 2021 12 06.
Artigo em Inglês | MEDLINE | ID: mdl-34873654

RESUMO

A novel electrochemical sensor has been fabricated for sensitive determination of zearalenone (ZEA) in food samples based on molybdenum disulfide quantum dots (MoS2 QDs) and two-dimensional titanium carbide (2D-Ti3C2Tx MXene) co-modified multi-walled carbon nanotube (MWCNT) heterostructure. Physical and electrochemical characterizations reveal that 2D-Ti3C2Tx and MoS2 QDs co-modified MWCNTs yields synergistic signal amplification effect, together with large specific surface area and excellent conductivity for the heterostructure, endowing the developed sensor with high detection performance to ZEA. Under optimized conditions, the sensor shows a wide linear range from 3.00 to 300 ng mL-1 and a low limit of detection (LOD) of 0.32 ng mL-1, which is far lower than the maximum residue limits (MRLs) settled by the European Commission. In addition, it exhibits excellent selectivity, high reproducibility with a relative standard deviation (RSD) of 1.1%, and good repeatability (RSD 1.1%). In real sample analysis recoveries ranged from 94.8 to 105% showing the proposed electrochemical sensor has high potential in practical applications. This work presents an effective and valuable pathway for the use of novel heterostructure in the biosensing field.


Assuntos
Dissulfetos/química , Técnicas Eletroquímicas/métodos , Molibdênio/química , Nanotubos de Carbono/química , Pontos Quânticos/química , Titânio/química , Zearalenona/análise , Técnicas Eletroquímicas/instrumentação , Eletrodos , Farinha/análise , Contaminação de Alimentos/análise , Limite de Detecção , Reprodutibilidade dos Testes , Zea mays/química
17.
Biomed Res Int ; 2021: 6679082, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34195278

RESUMO

The aim of our study was to investigate the effects of single-dose Ougan (Citrus reticulata cv. Suavissima) juice application on the pharmacokinetics of erlotinib in vivo. Twelve Sprague-Dawley rats were randomly divided into the Ougan juice and control groups (n = 6 each). The rats were given a single dose of 1 mL/100 g Ougan juice or 1 mL/100 g normal saline (NS) by intragastric administration, followed by a single oral administration of 20 mg/kg erlotinib. The plasma concentration of erlotinib in rats was determined using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Erlotinib-d6 was used as the internal standard for chromatographic analysis on the UPLC BEH C18 analysis column (2.1 mm × 50 mm, 1.7 µm). The mobile phase was composed of acetonitrile and 0.1% formic acid eluting by gradient. Different pharmacokinetic (PK) parameters of erlotinib were calculated. The Ougan juice promoted the absorption of erlotinib and reduced the clearance of the drug. The area under the curve of erlotinib in the single-dose Ougan juice pretreatment group was approximately 1.87 times higher, and the maximum blood concentration (Cmax) was approximately 1.34 times higher than that in the control group. The mean residence time of erlotinib in the Ougan juice group was larger, and the clearance rate was smaller than those in the control group; the difference was statistically significant (P < 0.05). Ougan juice affected the PK spectrum of erlotinib in rats by improving the bioavailability of the drug and significantly increasing its plasma concentration.


Assuntos
Citrus/metabolismo , Cloridrato de Erlotinib/farmacocinética , Sucos de Frutas e Vegetais , Animais , Cromatografia Líquida , Citocromo P-450 CYP3A/metabolismo , Interações Medicamentosas , Cloridrato de Erlotinib/sangue , Ratos , Ratos Sprague-Dawley , Reprodutibilidade dos Testes , Estômago/efeitos dos fármacos , Espectrometria de Massas em Tandem
18.
Environ Sci Pollut Res Int ; 28(48): 69301-69313, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34296409

RESUMO

The heterogeneous photo-Fenton-like process is emerging as a promising treatment of antibiotics-containing wastewater. The preparation of new efficient and stable catalysts is one of the research fields. A composite catalyst, prussian blue (PB) modified CeO2 was prepared, characterized, and applied for photo-Fenton oxidation of norfloxacin (NOR) in this study. It was found that chemical doping of PB leaded to more oxygen vacancies and increased the surface area of CeO2 obviously. PB/CeO2 with more Ce3+ facilitated electron transfer between Fe3+/Fe2+ with Ce3+/Ce4+. PB could also improve the separation rate of photoexcited electron-hole pairs in CeO2 nanostructures. When the doping ratio of PB and CeO2 was 10%, PB/CeO2 show the highest catalytic degradation ability and 88.93% of NOR could be degraded within 30 min. PB/CeO2 composite showed well reactivity at the wide pH value range of 3-8. The reusable experiments and low iron dissolution with less than 1 mg/L indicated that PB/CeO2 could be employed as an efficient heterogeneous photo-Fenton-like catalyst in organic contaminants degradation.


Assuntos
Norfloxacino , Água , Catálise , Ferrocianetos , Peróxido de Hidrogênio , Oxirredução
19.
Brain Res Bull ; 172: 108-119, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-33932488

RESUMO

Pathophysiological mechanisms of chronic subdural hematoma (CSDH) involve localized inflammation, angiogenesis, and dysregulated coagulation and fibrinolysis. The scarcity of reproducible and clinically relevant animal models of CSDH hinders further understanding the underlying pathophysiology and improving new treatment strategies. Here, we developed a novel rat model of CSDH using extracellular matrices (Matrigel) and brain microvascular endothelial cell line (bEnd.3 cells). One hundred-microliter of Matrigel-bEnd.3 cell (106 cells per milliliter) mixtures were injected into the virtual subdural space of elderly male Sprague-Dawley rats. This approach for the first time led to a spontaneous and expanding subdural hematoma, encapsulated by internal and external neomembranes, formed as early as 3 d, reached its peak at 7 d, and lasted for more than 14 d, mimicking the progressive hemorrhage observed in patients with CSDH. The external neomembrane and hematoma fluid involved numerous inflammatory cells, fibroblasts, and highly fragile neovessels. Furthermore, a localized pathophysiological process was validated as evidenced by the increased expressions of inflammatory and angiogenic mediators in external neomembrane and hematoma fluid rather than in peripheral blood. Notably, the specific expression profiles of these mediators were closely associated with the dynamic changes in hematoma volume and neurological outcome. In summary, the CSDH model described here replicated the characteristics of human CSDH, and might serve as an ideal translational platform for preclinical studies. Meanwhile, the crucial roles of angiogenesis and inflammation in CSDH formation were reaffirmed.


Assuntos
Hematoma Subdural Crônico/patologia , Inflamação/patologia , Neovascularização Patológica/patologia , Espaço Subdural/patologia , Animais , Modelos Animais de Doenças , Hematoma Subdural Crônico/diagnóstico por imagem , Inflamação/diagnóstico por imagem , Imageamento por Ressonância Magnética , Masculino , Neovascularização Patológica/diagnóstico por imagem , Ratos , Ratos Sprague-Dawley , Espaço Subdural/diagnóstico por imagem
20.
Environ Res ; 195: 110840, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33587946

RESUMO

Intimate coupling of visible-light photocatalysis and biodegradation (ICPB) offers potential for degrading chlorine dioxide bleaching wastewater. In this study, we reported a TiO2-coated sponge biofilm carrier with significant adhesion of TiO2 and the ability to accumulate biomass in its interior. Four mechanisms possibly acting in ICPB were tested separately: adsorption of chlorine dioxide bleaching wastewater to the carrier, photolysis, photocatalysis, and biodegradation by the biofilm inside the carrier. The carrier had an adsorption capacity of 17% and 16% for CODcr and AOX, respectively, in the wastewater. The photodegradation rate of wastewater was very low and could be ignored. Both biodegradation (AOX 30.1%, CODcr 33.8%, DOC 26.2%) and photocatalysis (AOX 65.1%, CODcr 71.2%, DOC 62.3%) possessed a certain degradation efficiency of wastewater. However, the removal rate of AOX, CODcr, and DOC in wastewater treatment by protocol ICPB reached 80.3%, 90.5%, and 86.7%. FT-IR and GC-MS analysis showed that the ICPB system had photocatalytic activity on the surface of the porous carrier in vitro, which could transform organic into small molecules for microbial utilization or complete mineralization. Moreover, the biofilm in the interior of the TiO2-coated sponge carrier could mineralize the photocatalytic products, which enhanced the removal of AOX, CODcr, and DOC by more than 15.2%, 20.0%, and 24.0%, respectively. The biofilm in the carrier of the ICPB system evolved, enriched in Proteobacteria, Chloroflexi, Bacteroidetes, and Actinobacteria, microorganisms known to play active roles in the biodegradation of papermaking wastewater.


Assuntos
Titânio , Biodegradação Ambiental , Catálise , Compostos Clorados , Óxidos , Fotólise , Espectroscopia de Infravermelho com Transformada de Fourier
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