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2.
Transl Psychiatry ; 11(1): 374, 2021 07 05.
Artigo em Inglês | MEDLINE | ID: mdl-34226510

RESUMO

Suffering from COVID-19 and witnessing the suffering and deaths of patients with COVID-19 may place frontline healthcare workers (HCWs) at particularly high risk for posttraumatic stress disorder (PTSD); however, few data are available on the clinical characteristics of PTSD among frontline HCWs who survived COVID-19 ("surviving HCWs" hereafter). The present study examined the prevalence, correlates, and clinical symptoms of possible PTSD in surviving HCWs 6 months after the COVID-19 outbreak in China. A total of 291 surviving HCWs and 42 age- and gender-matched COVID-19-free frontline HCWs (control group) were recruited and administered the Chinese Essen Trauma Inventory, which was used to assess the presence of possible PTSD according to DSM-IV-TR criteria. Survivors' clinical data and characteristics of exposure to COVID-19 were collected via self-report questionnaires. Surviving HCWs had significantly higher rates of possible PTSD than controls (19.9% vs. 4.8%, P = 0.017). Correlates of PTSD in survivors were ICU admission (OR = 8.73, P = 0.003), >10 respiratory symptoms during the most symptomatic period of COVID-19 (OR = 3.08, P = 0.006), the residual symptom of dizziness (OR = 2.43, P = 0.013), the residual symptom of difficult breathing (OR = 2.23, P = 0.027), life in danger due to COVID-19 (OR = 16.59, P = 0.006), and exposure to other traumatic events (OR = 2.94, P = 0.035). Less commonly seen PTSD symptoms in survivors were having nightmares about the event (34.5%), suddenly feeling like they were living through the event suddenly (25.9%), being unable to remember an important part of the event (32.8%), and overalertness (31.0%). Nearly one-fifth of the surviving HCWs had possible PTSD 6 months after the COVID-19 outbreak. Mental health services for this vulnerable population should include periodic screening for PTSD, expanded social support, and, when necessary, psychotherapy and psychopharmacological treatment.


Assuntos
COVID-19 , Transtornos de Estresse Pós-Traumáticos , China/epidemiologia , Surtos de Doenças , Pessoal de Saúde , Humanos , Prevalência , SARS-CoV-2 , Transtornos de Estresse Pós-Traumáticos/epidemiologia
3.
World J Pediatr ; 16(3): 232-239, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32333248

RESUMO

In the early February, 2020, we called up an experts' committee with more than 30 Chinese experts from 11 national medical academic organizations to formulate the first edition of consensus statement on diagnosis, treatment and prevention of coronavirus disease 2019 (COVID-19) in children, which has been published in this journal. With accumulated experiences in the diagnosis and treatment of COVID-19 in children, we have updated the consensus statement and released the second edition recently. The current version in English is a condensed version of the second edition of consensus statement on diagnosis, treatment and prevention of COVID-19 in children. In the current version, diagnosis and treatement criteria have been optimized, and early identification of severe and critical cases is highlighted. The early warning indicators for severe pediatric cases have been summarized which is utmost important for clinical practice. This version of experts consensus will be valuable for better prevention, diagnosis and treatment of COVID-19 in children worldwide.


Assuntos
Infecções por Coronavirus , Coronavirus , Pandemias , Pneumonia Viral/epidemiologia , Betacoronavirus , COVID-19 , Criança , Consenso , Humanos , SARS-CoV-2
5.
BMC Musculoskelet Disord ; 16: 315, 2015 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-26497060

RESUMO

BACKGROUND: The responses of immunological factors to different subtypes of Kawasaki disease (KD) remain poorly understood. METHODS: We recruited 388 patients with KD, 160 patients with infectious febrile disease and 85 normal children who served as control subjects. Both the levels and percentages of T lymphocyte subsets, natural killer cells (NK cells) and B cells were analyzed via flow cytometry. The levels of serum IgG, IgM, IgA and C3, C4 were assessed via velocity scatter turbidimetry. RESULTS: The most significant differences noted between the patients with infectious febrile disease and the normal children were the elevated levels of B cells, C3 and the ratio of CD4/CD8, and the decreased levels of CD8+ T cells and NK cells, as well as the moderate increase in the absolute value of the CD3+ cells. The decreased T cell levels and the elevated B cell levels were helpful in distinguishing typical KD from atypical KD; the elevated T cell levels, the elevated NK cell and B cell levels and the decreased B cell levels were helpful in predicting the effectiveness of IVIG; low C3 and C4 levels were linked with prodromal infections. CONCLUSIONS: Lymphocytes subsets and complement markers may be useful in differentiating among the different subtypes of KD and in helping clinicians understand the pathophysiology of KD.


Assuntos
Síndrome de Linfonodos Mucocutâneos/imunologia , Adolescente , Estudos de Casos e Controles , Criança , Pré-Escolar , Feminino , Febre/imunologia , Humanos , Imunoglobulinas/sangue , Lactente , Subpopulações de Linfócitos , Masculino , Síndrome de Linfonodos Mucocutâneos/sangue
6.
J Huazhong Univ Sci Technolog Med Sci ; 35(2): 283-290, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25877366

RESUMO

Inpatients in the intensive care unit (ICU) are at high risk for healthcare-associated infections (HAIs). In the current study, a bundle of interventions and measures for preventing and controlling HAIs were developed and implemented in the ICU by trained personnel, and the impact of the bundle was evaluated. The incidence of HAIs, the adjusted daily incidence of HAIs and the incidence of three types of catheter-related infections before and after the bundle implementation were compared. The execution rate of the bundle for preventing and controlling ventilator-associated pneumonia (VAP) was increased from 82.06% in 2012 to 96.88% in 2013. The execution rate was increased from 83.03% in 2012 to 91.33% in 2013 for central line-associated bloodstream infection (CLABSI), from 87.00% to 94.40% for catheter-associated urinary tract infection (CAUTI), and from 82.05% to 98.55% for multidrug-resistant organisms (MDROs), respectively. In total, 136 cases (10.37%) in 2012 and 113 cases (7.72%) in 2013 involved HAIs, respectively. Patients suffered from infection of the lower respiratory tract, the most common site of HAIs, in 134 cases (79.29%) in 2012 and 107 cases (74.30%) in 2013 respectively. The incidence of VAP was 32.72‰ and 24.60‰, the number of strains of pathogens isolated was 198 and 173, and the number of MDROs detected in the ICU was 91 and 74 in 2012 and 2013, respectively. The percentage of MDROs among the pathogens causing HAIs was decreased in each quarter of 2013 as compared with the corresponding percentage in 2012. In 2013, the execution rate of the bundle for preventing and controlling HAIs was increased, whereas the incidence of HAIs and VAP decreased as compared with that in 2012.


Assuntos
Infecção Hospitalar/prevenção & controle , Unidades de Terapia Intensiva , Guias de Prática Clínica como Assunto , Adulto , Idoso , China , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
7.
Int J Endocrinol ; 2013: 453898, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24348555

RESUMO

Objective. To study the effects of estrogen on colon polyp formation, proliferation, and angiogenesis on a rat model of colon cancer induced by dimethylhydrazine (DMH). Methods. Thirty-six female ovariectomized (OVX) rats were randomly divided into 3 groups: (I) control group (administrated with vehicles weekly), (II) DMH group (administrated with DMH weekly), and (III) DMH + E2 group (administrated with DMH and 17ß-estradiol weekly). The incidence, volumes, and multiplicity of colon polyps in each group were evaluated. The microvessel density (MVD), the expressions of Proliferating Cell Nuclear Antigen (PCNA), and the expressions of HIF-1 α and VEGF in polyps were detected in each group. Results. Estrogen reduced the multiplicity, volumes, and the PCNA expressions of DMH-induced colon polyps. The MVD in DMH + E2 group was significantly lower than that in DMH group. Estrogen treatment decreased the HIF-1 α and VEGF expressions at both mRNA and protein level. Conclusion. Estrogen replacement was protective for ovariectomized rats from DMH-induced carcinogenesis, and one of the mechanisms for this was due to estrogen's inhibitive effects on blood vessel formation by downregulating VEGF and HIF-1 α expressions.

8.
Cell Biochem Biophys ; 67(3): 965-8, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23543427

RESUMO

To study the response of the cardiovascular system, to exercise tolerance in-patients over 75 years old with coronary heart disease (CHD), and to evaluate the significance of the parameters of the treadmill exercise test (TET). 110 patients received TET and coronary artery angiography. They were divided into two groups: the elderly patients group included 50 patients over 75 years old, and the control group included 60 patients under 60 years old. (1) With aging, there were much more CHD patients in the positive TET (P < 0.05) than in the negative TET (P > 0.05). (2) The parameters of TET for the elderly CHD patients group, included exercise time, peak heart rate, and the onset of ST depression, were lower than the control group (P < 0.05). There was no statistical significance between the two groups in the extent and duration of ST depression (P < 0.05). (1) In TET, the elderly patients had the higher diagnostic value on CHD. (2) The elderly patients with CHD had the lower endurance to exercise test.


Assuntos
Doença das Coronárias/fisiopatologia , Teste de Esforço , Idoso , Idoso de 80 Anos ou mais , Angiografia Coronária , Feminino , Frequência Cardíaca , Humanos , Masculino , Pessoa de Meia-Idade
10.
Huan Jing Ke Xue ; 28(10): 2336-41, 2007 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-18269002

RESUMO

MBFGA1, which was produced by Paenibacillus polymyxa GA1, was used to investigate its flocculating capability in the following waste water, the liquid of kaolin, soil suspended liquid, coal-washing wastewater and the landfill leachate with orthogonal experiments, the inspection of granularity and the scanning electron microanalysis. Meanwhile the anthrone reaction and infrared spectrum are also applied to identify its main component. In the optimal flocculating conditions obtained from the orthogonal experiments, the flocculating rates of the liquid of kaolin, soil suspended liquid, coal-washing wastewater and the landfill leachate were 99.53%, 99.50%, 98.2% and 75.60%, respectively, and the sedimentation velocities of the above four kinds of flocculants were 0.03 m/s, 0.025 m/s, 0.025 m/s and 0.005 m/s, respectively. In addition, the distribution of granularity has changed after flocculation. The size of the average granularity of soil became bigger after flocculation, and reached over 10 microm, but for the other three kinds of granularity just the opposite phenomenon has occurred, and their sizes were all less than 10 microm. From the photos of scan electron microanalysis of four types of granules, it could be drawn that there were no obvious differences in the appearance. It was the same adsorptive mode between MBFGA1 and different flocculating particles, that is the function of electrovalent bond, tested by EDTA, HC1 and urea. The main component of MBFGA1 was polysaccharide, which was determined by anthrone reaction, and it contained a lot of carboxyl and hydroxyl groups examined by infrared spectrum analysis. From the above results, it could be presumed that MBFGA1 can be the best flocculant for the granule-highly-concentrated waste water and the dominative flocculating mechanism of the flocculant was mechanism of adsorption-bridge.


Assuntos
Bactérias/metabolismo , Polissacarídeos Bacterianos/química , Microbiologia do Solo , Eliminação de Resíduos Líquidos/métodos , Adsorção , Meios de Cultura , Floculação , Caulim/química , Polissacarídeos Bacterianos/biossíntese , Solo , Poluentes Químicos da Água/química , Purificação da Água
11.
Artigo em Chinês | MEDLINE | ID: mdl-16566217

RESUMO

OBJECTIVE: To study the expression of hepatic Bcl-2 and Bax proteins in mice infected with Schistosoma japonicum and the role of pentoxifylline (PTX) in the expression. METHODS: Forty mice were randomly divided into 4 groups: one normal control group,mice in the other three groups were all infected each with 25 cercariae, the infected control group was fed for 10 weeks after infection, and 2 weeks after infection, the high dose PTX group was given PTX 360 mg/(kg x d) for 8 weeks and the low dose PTX group was given PTX 180 mg/(kg x d)also for 8 weeks. At the end of 10 weeks all the mice were killed. Bcl-2 and Bax proteins expression was detected by immunohistochemisty. RESULTS: Compared with the normal control group, the expression of Bcl-2 and Bax was significantly higher in the infected control group (P < 0.05). Bcl-2 was significantly higher in high (dose PTX group than in the infected control group and in low dose PTX group (P < 0.05). However there was no significant difference in the expression of Bax among the groups (P > 0.05). CONCLUSION: PTX treatment can significantly increase the expression of Bcl-2 in liver tissue of schistosome-infected mice in a dose-dependent manner, and may play a role against liver inflammation and schistosomiasis-related liver fibrosis.


Assuntos
Fígado/metabolismo , Pentoxifilina/farmacologia , Inibidores de Fosfodiesterase/farmacologia , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Esquistossomose Japônica/tratamento farmacológico , Proteína X Associada a bcl-2/metabolismo , Animais , Relação Dose-Resposta a Droga , Feminino , Imuno-Histoquímica , Fígado/patologia , Camundongos , Pentoxifilina/administração & dosagem , Inibidores de Fosfodiesterase/administração & dosagem , Distribuição Aleatória , Esquistossomose Japônica/metabolismo , Esquistossomose Japônica/patologia
12.
World J Gastroenterol ; 9(7): 1594-7, 2003 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-12854171

RESUMO

AIM: To study the effects of carbon dioxide on the metastatic capability of cancer cells, and to compare them with that of nitrogen. METHODS: The colon cancer cell CCL-228 was treated with 100 % carbon dioxide or nitrogen at different time points and then cultured under normal condition. Twelve hours after the treatment, the survival rates of suspension cells and the expressions of e-cadherin and VEGF were examined. RESULTS: After 60 min of carbon dioxide and longer time of nitrogen treatment, the suspended cells increased and the expression of e-cadherin decreased while the expression of VEGF was enhanced significantly. And the effects of nitrogen were similar to, but weaker than, those of carbon dioxide. CONCLUSION: Carbon dioxide may improve the metastatic capability of cancer cells and its effects are significantly stronger than that of nitrogen. A sequential use of carbon dioxide and nitrogen in pneumoperitoneum may take the advantage of both gases.


Assuntos
Caderinas/biossíntese , Dióxido de Carbono/farmacologia , Neoplasias do Colo , Nitrogênio/farmacologia , Fator A de Crescimento do Endotélio Vascular/biossíntese , Adesão Celular/efeitos dos fármacos , Divisão Celular/efeitos dos fármacos , Linhagem Celular Tumoral/citologia , Linhagem Celular Tumoral/efeitos dos fármacos , Linhagem Celular Tumoral/metabolismo , Humanos
13.
World J Gastroenterol ; 9(1): 152-4, 2003 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-12508372

RESUMO

AIM: To study the effects of pentoxifylline (PTX) on the content of hepatic TGF-beta1, type I and type III collagen in schistosomiasis japonica mice with liver fibrosis and its mechanism of anti-fibrosis. METHODS: Forty mice with schistosomiasis were divided into four groups: one group as control without any treatment, other three were treated with Praziquantel 500 mg/(kg x d)for 2 d, high dose PTX 360 mg/(kg x d) for 8 wk, and low dose PTX 180 mg/(kg x d) for 8 wk respectively. Immunohistochemical technique and multimedia color pathographic analysis system were applied to observe the content change of hepatic TGF-beta1, type I and type III collagen in schistosomiasis japonica mice with liver fibrosis before and after PTX treatment. RESULTS: Effects of PTX on the content change of hepatic TGF-beta1, type I and type III collagen in schistosomiasis japonica mice with liver fibrosis were related to the dosage of PTX, high dose PTX treated group could significantly reduce the content of TGF-beta1 (0.709+/-0.111), type I (0.644+/-0.108) and type III (0.654+/-0.152) collagen compared with those of control group (0.883+/-0.140, 0.771+/-0.156, 0.822+/-0.129) with statistical significance (P<0.05). Low dose PTX could also reduce the hepatic content of TGF-beta1 (0.752+/-0.152), type I (0.733+/-0.117) and type III (0.788+/-0.147) collagen, but without statistical significance (P>0.05). Both high dose and low dose PTX groups have significant differences on the content of TGF-beta1, type I and type III collagen (P<0.05, P<0.05, P<0.01, respectively). CONCLUSION: High dose of PTX treatment could reduce the content of hepatic TGF-beta1, type I and type III collagen significantly in schistosomiasis japonica mice with liver fibrosis, and thus plays its role of antifibrosis.


Assuntos
Colágeno Tipo III/metabolismo , Colágeno Tipo I/metabolismo , Fígado/efeitos dos fármacos , Fígado/patologia , Pentoxifilina/farmacologia , Esquistossomose Japônica/metabolismo , Fator de Crescimento Transformador beta/metabolismo , Animais , Anti-Helmínticos/farmacologia , Feminino , Fígado/metabolismo , Cirrose Hepática/metabolismo , Cirrose Hepática/parasitologia , Cirrose Hepática/patologia , Camundongos , Praziquantel/farmacologia , Distribuição Aleatória , Esquistossomose Japônica/complicações , Esquistossomose Japônica/patologia , Fator de Crescimento Transformador beta1
14.
Artigo em Chinês | MEDLINE | ID: mdl-12568020

RESUMO

OBJECTIVE: To study the effect of pentoxifylline (PTX) on the content of hepatic TGF-beta 1, type I and type III collagen in schistosome-infected mice with liver fibrosis. METHODS: Forty mice with schistosomiasis were divided into four groups: one group as control without any treatment, other three were treated with praziquantel 500 mg/(kg.d) for 2 d, high dose PTX 360 mg/(kg.d) for 8 wk, and low dose PTX 180 mg/(kg.d) for 8 wk respectively. Immunohistochemical technique and multimedia color pathographic analysis system were applied to observe the content of hepatic TGF-beta 1, type I and type III collagen in mice infected with S. japonicum before and after treatment. RESULTS: The effect of PTX on the content of hepatic TGF-beta 1, type I and type III collagen in mice was related to the dosage of PTX. High dose PTX treatment significantly reduced the content of TGF-beta 1, type I and type III collagen compared to the control (P < 0.01), whereas no difference was found between the group of low dose PTX treatment and control (P > 0.05). CONCLUSION: High dose PTX treatment could reduce the content of hepatic TGF-beta 1, type I and type III collagen significantly in schistosome-infected mice with liver fibrosis.


Assuntos
Colágeno Tipo III/metabolismo , Colágeno Tipo I/metabolismo , Cirrose Hepática Experimental/metabolismo , Pentoxifilina/farmacologia , Inibidores de Fosfodiesterase/farmacologia , Esquistossomose Japônica/metabolismo , Fator de Crescimento Transformador beta/metabolismo , Animais , Relação Dose-Resposta a Droga , Feminino , Fígado/metabolismo , Cirrose Hepática Experimental/parasitologia , Camundongos , Pentoxifilina/administração & dosagem , Inibidores de Fosfodiesterase/administração & dosagem , Esquistossomose Japônica/complicações , Fator de Crescimento Transformador beta1
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