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1.
Mol Med Rep ; 21(3): 1233-1241, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32016469

RESUMO

The aim of the present study was to investigate the antioxidant mechanisms of dexmedetomidine against lung injury during intestinal ischemia reperfusion (IIR) in rats. The model of IIR­induced acute lung injury was established by occluding the superior mesenteric artery (SMA) for 1 h and reperfusing for 2 h using Sprague­Dawley rats. Pathological examination was used to assess the extent of the lung injury. Oxidative stress was evaluated by measuring malondialdehyde, myeloperoxidase and superoxide dismutase in the lung and plasma. The proinflammatory cytokines tumor necrosis factor­α and interleukin­6 were determined via an enzyme­linked immunosorbent assay. The mRNA and protein expression of nuclear factor­erythroid 2 related factor 2 (Nrf2) and heme oxygenase 1 (HO­1) were determined using a reverse transcription­quantitative polymerase chain reaction and western blotting. Pretreatment with dexmedetomidine significantly inhibited the oxidative stress response and proinflammatory factor release caused by IIR compared with the normal saline group (MDA and SOD in lung and plasma, P<0.05; MPO, IL­1ß and TNF­α in lung and plasma, P<0.05). Dexmedetomidine improved pulmonary pathological changes in IIR rats compared with the normal saline group. Investigations into the molecular mechanism revealed that dexmedetomidine increased the expression levels of Nrf2 and HO­1 via activating α2 adrenergic receptors compared with the normal saline group. The antagonism of α2 adrenergic receptors may reverse the protective effect of dexmedetomidine on lung injury during IIR, including decreasing the expression levels of Nrf2 and HO­1, elevating the oxidative stress response and increasing the proinflammatory factor release. In conclusion, pretreatment with dexmedetomidine demonstrated protective effects against lung injury during IIR via α2 adrenergic receptors. The Nrf2/HO­1 signaling pathway may serve a function in the protective effect of dexmedetomidine.

2.
J Colloid Interface Sci ; 566: 383-393, 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-32018178

RESUMO

Metal-organic frameworks (MOFs) are an emerging class of hybrid inorganic-organic porous materials used in various fields. Considering their excellent performance, MOFs have a considerable application potential in sustainable agriculture. Research projects of MOFs-based platforms for plant protection and nutrition have just started. Environmental stimuli-responsive pesticide release is highly desirable for improved efficacy and decreased side effects. Iron-based MOFs (Fe-MOFs) have a considerable prospect in agriculture as multifunctional materials both for pesticide delivery and plant nutrient replenishment because iron is an essential micronutrient for crop growth. In this work, a simple octahedral Fe-MOFs built from trimers of iron octahedra linked by 1, 3, 5-benzenetricarboxylate (Fe-MIL-100) have been prepared as carriers for fungicide azoxystrobin. Due to the high surface area of 2251 m2/g, the loading content of azoxystrobin into Fe-MIL-100 is satisfactory up to 16.2%. Azoxystrobin-loaded Fe-MOFs (AZOX@Fe-MIL-100) exhibit a pH-responsive initial burst and a subsequent sustained release pattern. Moreover, AZOX@Fe-MIL-100 exhibits good fungicidal activities against two pathogenic fungi-wheat head scab (Fusarium graminearum) and tomato late blight (Phytophthora infestans). The nutritional function of Fe-MIL-100 as iron micronutrient for the enhanced wheat growth was also observed. This research explores the feasibility of MOFs as a platform for potential application in sustainable plant protection.

3.
EuroIntervention ; 2020 02 11.
Artigo em Inglês | MEDLINE | ID: mdl-32038026

RESUMO

AIMS: This analysis presents the final 5-year results of the I-LOVE-IT 2 trial, a noninferiority study comparing BP- sirolimus-eluting stent (SES) with DP-SES in patients with coronary artery disease. METHODS AND RESULTS: Overall, 2737 Chinese patients eligible for coronary stenting were treated with BP- or DP-SES in a 2:1 ratio. Patients who were randomized to BP-SES group were additionally re-randomized to receive either 6-month or 12-month dual antiplatelet therapy (DAPT) in a 1:1 ratio. The primary endpoint was 12-month target lesion failure (TLF: cardiac death, target vessel myocardial infarction, or clinically indicated target lesion revascularization). At five years, overall follow-up rate was 90.8%, and the cumulative incidence of TLF as the primary endpoint was similar between BP-SES and DP-SES (hazard ratio [HR]: 1.01; 95% confidence interval [CI]: 0.79 to 1.28), as was that for patient-oriented composite endpoint (PoCE: all-cause death, all MI and any revascularization) (HR: 1.03, 95% CI: 0.86 to 1.23), or definite/probable ST (HR: 1.11, 95% CI: 0.70 to 1.77). Cumulative events also were similar between 6-month DAPT and 12-month DAPT groups after BP-SES implantation. CONCLUSIONS: I-LOVE-IT 2 has shown that the 5-year safety and efficacy of BP-SES and DP-SES were similar, as were those between 6-month and 12-month of DAPT after BP-SES implantations.

4.
Neuroreport ; 31(4): 319-324, 2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-32058434

RESUMO

Painful diabetic neuropathy may associate with nerve morphological plasticity in both peripheral and central nervous system. The aim of this study was to determine numerical changes of myelinated fibers in the spinothalamic tract region and oligodendrocytes in the spinal dorsal horn of rats with painful diabetic neuropathy and the effects of metformin on the above changes. Male Sprague-Dawley rats were randomly allocated into the control group (n = 7), the painful diabetic neuropathy group (n = 6) and the painful diabetic neuropathy treated with metformin group (the PDN + M group, n = 7), respectively. Twenty-eight days after medication, numbers of myelinated fibers in the spinothalamic tract and oligodendrocytes in the spinal dorsal horn were estimated by the optical disector (a stereological technique). Compared to the control group, number of myelinated fibers in the spinothalamic tract increased significantly in the painful diabetic neuropathy and PDN + M group, compared to the painful diabetic neuropathy group, number of myelinated fibers decreased in the PDN + M group (P < 0.05). As the oligodendrocyte in the spinal dorsal horn was considered, its number increased significantly in the painful diabetic neuropathy group compared to the control and the PDN + M group (P < 0.05), there was no significant difference between the control and the PDN + M group (P > 0.05). Our results indicate that painful diabetic neuropathy is associated with a serial of morphometric plasticity in the rat spinal cord including the numerical increase of the myelinated fibers in the spinothalamic tract and the oligodendrocytes in the spinal dorsal horn. The analgesic effect of metformin against painful diabetic neuropathy might be related to its adverse effects on the above morphometric plasticity.

5.
Phytomedicine ; 68: 153179, 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32062328

RESUMO

BACKGROUND: Intestinal epithelial barrier dysfunction, which involves myosin light chain kinase (MLCK) activation, contributes to the occurrence and progression of inflammation in inflammatory bowel disease (IBD). Wogonoside helps maintain intestinal homeostasis in mice with dextran sulfate sodium (DSS)-induced colitis, but it is unclear whether it modulates intestinal barrier function. PURPOSE: Here, we demonstrate that wogonoside protects against intestinal barrier dysfunction in colitis via the MLCK/pMLC2 pathway both in vivo and in vitro. METHODS: Caco-2 cell monolayers treated with the proinflammatory cytokine TNF-α showed barrier dysfunction and were assessed in the absence and presence of wogonoside for various physiological, morphological, and biochemical parameters. Colitis was induced by 3% DSS in mice, which were used as an animal model to explore the pharmacodynamics of wogonoside. We detected MLCK/pMLC2 pathway proteins via western blot analysis, assessed the cytokines IL-13 and IFN-γ via ELISA, tested bacterial translocation via fluorescence in situ hybridization (FISH) and a proper sampling of secondary lymphoid organs for bacterial culture. In addition, the docking affinity of wogonoside and MLCK was observed with DS2.5 software. RESULTS: Wogonoside alleviated the disruption of transepithelial electrical resistance (TER) in TNF-α exposured Caco-2 cell; FITC-dextran hyperpermeability; loss of the tight junction (TJ) proteins occludin, ZO-1 and claudin-1 in Caco-2 cell monolayers; and bacterial translocation in colitic mice. Moreover, wogonoside reduced the levels of the proinflammatory cytokines IL-13 and IFN-γ to maintain intestinal immune homeostasis. Transmission electron microscopy (TEM) confirmed that wogonoside ameliorated the destruction of intestinal epithelial TJs. Wogonoside not only inhibited the cytoskeletal F-actin rearrangement induced by TNF-α, stabilized the cytoskeletal structure, suppressed MLCK protein expression, and reduced MLC2 phosphorylation. In addition, the results of molecular docking analysis showed that wogonoside had a high affinity for MLCK and formed hydrogen bonds with the amino acid residue LYS261 and π bonds with LYS229. CONCLUSION: Collectively, our study indicates that wogonoside alleviates colitis by protecting against intestinal barrier dysfunction, and the potential mechanism may involve regulation of TJs via the MLCK/pMLC2 signaling pathway. Meanwhile, our study also explains the success of S. baicalensis in the treatment of ulcerative colitis (UC).

6.
Chemosphere ; 249: 126166, 2020 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-32062560

RESUMO

Rice grains are a methylmercury (MeHg) intake route for humans, especially in certain mercury (Hg)-contaminated areas. For rice plant growth, animal manure is commonly used as an organic fertilizer; however, its role in the formation of MeHg in paddy soils remains poorly understood. The aims of this study were thus to explore 1) the effect of chicken manure (CH) and cow manure (CO) addition on the production of soil MeHg and the accumulation of MeHg in rice plants and 2) the mechanism by which CH and CO addition affect the bioaccumulation of MeHg in grains. A pot experiment with different levels of CH and CO was carried out with newly deposited Hg-contaminated paddy soil. Two microcosm experiments were performed to explore the associated mechanisms. The results of the pot experiment showed that 0.1-1% CH and CO addition promoted the biomass of rice plants by 10-23% and increased the soil MeHg concentration by 34-143%, which exhibited a significant positive correlation with brown rice MeHg content. Organic fertilizer addition significantly increased MeHg bioaccumulation in rice plants and the ratio of MeHg to total Hg (THg) in brown rice. Organic fertilizer also increased the abundance of microbial methylators. The results of the microcosm experiments showed that organic fertilizer addition enhanced dissolved THg concentrations in soil and consequently increased the soil MeHg concentration. These results suggested that applying organic fertilizer to newly deposited Hg-contaminated soil may increase MeHg accumulation in grains due to enhanced Hg release and microbial methylator activity, leading to environmental health concerns.

7.
Thorac Cancer ; 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32012484

RESUMO

BACKGROUND: There is no consensus on the definition or recommended radiotherapy treatment of ultracentral non-small cell lung cancer (NSCLC). Here, we report our institution's experience in treating ultracentral lung cancer patients with stereotactic ablative radiotherapy (SABR) of 60 Gy in eight fractions. METHODS: We retrospectively reviewed the outcomes of 21 ultracentral NSCLC patients treated with 60 Gy SABR in eight fractions. We defined ultracentral lung cancer as the planning target volume (PTV) directly abutting or overlapping central structures, including the proximal bronchial tree, heart, and great vessels but not the esophagus. The Kaplan-Meier method was used to estimate overall survival (OS), progression-free survival (PFS) and local control (LC). Toxicity was scored per the CTCAE v4.03. RESULTS: The median follow-up time was 15 months, and the median OS was 15 months. The one- and two-year OS rates were 87.5% and 76.6%, respectively. The one- and two-year PFS rates were 71.1% and 64.0%, respectively. The one- and two-year LC rates were 92.9% and 92.9%, respectively. The rate of grade 2 treatment-related toxicities was 19.1%. There was no grade ≥ 3 treatment-related toxicity. CONCLUSION: SABR of 60 Gy in eight fractions is feasible for ultracentral NSCLC.

8.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 34(2): 157-161, 2020 Feb 15.
Artigo em Chinês | MEDLINE | ID: mdl-32030944

RESUMO

Objective: To identify effect of quantitative indicators of ilium height on approach of percutaneous endoscopic lumbar discectomy (PELD) treatment in patients with L 5, S 1 lumbar disc herniation. Methods: A retrospective study between May 2014 and March 2016 was conducted, including 100 patients with disc herniation at L 5, S 1, who were initially enrolled for the PELD treatment. Among them, 66 patients were successfully treated with PELD (group A), and the other 34 patients failed to perform puncture, catheterization, or microscopical operation due to the influence of iliac bone and other peripheral bone structures and treated with alternative surgical plans. By analyzing the X-ray films of lumbar vertebrae (including bilateral ilium) of the two groups before operation, the concept of ilium height rate and ilium angle rate was put forward innovatively. The ilium height rate and ilium angle rate of the two groups were measured and compared, and the diagnostic critical points of ilium height rate and ilium angle rate were determined by ROC curve analysis. Results: The ilium height rate was 0.61±0.09, 0.74±0.05 and the ilium angle rate was 0.66±0.08, 0.80±0.08 in groups A and B, respectively, showing significant differences between the two groups ( F=69.729, P=0.000; F=65.165, P=0.000). ROC curve analysis showed that the critical point of ilium height rate was 0.71 (area under ROC curve was 0.927, P=0.000), and the critical point of ilium angle rate was 0.75 (area under ROC curve was 0.965, P=0.000). Conclusion: PELD is not recommended for patients with L 5, S 1 intervertebral disc herniation, when the ilium height rate is greater than 0.71 and/or the ilium angle rate is greater than 0.75. Other surgical plans such as transpedicular approach, transpedicular approach, or open surgery, should be recommended to reduce the risk of surgery and the pain of patients.

9.
Microb Pathog ; : 104051, 2020 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-32045642

RESUMO

BACKGROUND: Salpingitis is a part of pelvic inflammation, which is a threat for women's health. With development of genetic engineering, circular RNAs were discovered to have connections with diseases of human. METHODS: RT-qPCR was for analyzing expressions of EIF3K, miR-139-5p and RNAs related to apoptosis. CCK-8 was applied for detecting cell viabilities. Expressions of proteins in autophagy and MAPK/NF-κB signaling pathway was detected by western blot. Luciferase report assay was used to make sure binding situation between circEIF3K and miR-139-5p in cells. RESULTS: Circular EIF3K expressed lower in inflammatory cells with suppressing cell viabilities and promoting apoptosis and autophagy. MiR-139-5p could bind circEIF3K and promoted cell viabilities. Meanwhile, miR-139-5p could suppress apoptosis and autophagy. Functions of overexpressed EIF3K were restored by miR-139-5p in cell viabilities, apoptosis and autophagy. Proteins in MAPK/NF-κB signaling pathway expressed higher in inflammatory cells and overexpressed EIF3K could down regulate expressions of proteins. Inhibition then was resumed by up-regulated miR-139-5p. After proteins in MAPK signaling pathway was activated, apoptosis and autophagy was suppressed. CONCLUSION: Circular EIF3K suppressed cell growth in salpingitis through inhibiting MAPK/NK-κB signaling pathway, which could be a potential factor for further studies in slpingitis.

10.
Reprod Sci ; 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-32046427

RESUMO

Polycystic ovary syndrome (PCOS) is one of the most common gynaecological endocrine disorders, and more than 60% of PCOS patients have varying degrees of insulin resistance (IR). The regulatory role of microRNAs (miRNAs) at post-transcriptional levels in human cumulus cells relating to IR in PCOS remains unclear. In this case-control study, 26 PCOS patients with IR (PCOS-IR) and 24 patients without IR (PCOS-control) were enrolled. We determined the differentially expressed miRNA and mRNA using next-generation sequencing technology, and these miRNAs and mRNAs were validated by quantitative real-time polymerase chain reaction (PCR). These miRNA regulating pathways (e.g., MAPK pathway) were analysed by bioinformatics analysis, and the Rap1b was demonstrated to be targeted by miR-612 based on quantitative real-time PCR, western blot and luciferase activity assay. A total of 59 known miRNAs and 617 differentially expressed genes were identified that differentially expressed between PCOS-IR and PCOS-control cumulus cells. Moreover, the potential regulating roles of miRNAs and their targeting genes in pathophysiology of IR and PCOS were analysed by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotation, and several key processes were enriched, such as MAPK activity. Furthermore, Rap1b, a regulator of the MAPK pathway, was demonstrated to be suppressed directly by miR-612 in PCOS-IR cumulus cells based on negative expression correlation validation, dual luciferase activity assay and reduction of Rap1b expression after miR-612 mimics transfection. Our results suggested that miRNAs and their targeted pathways in ovarian cumulus cells may play important roles in the aetiology and pathophysiology of PCOS with IR.

11.
Cancer Med ; 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-32048471

RESUMO

Tamoxifen-based hormone therapy is central for the treatment of estrogen receptor positive (ER+ ) breast cancer. However, the acquired tamoxifen resistance, typically co-exists with hypoxia, remains a major challenge. We aimed to develop a non-invasive, targeted ultrasound imaging approach to dynamically monitory of tamoxifen resistance. After we assessed acquired tamoxifen resistance in 235 breast cancer patients and a list of breast cancer cell lines, we developed poly(lactic-co-glycolic acid)-poly(ethylene glycol)-carbonic anhydrase IX mono antibody nanobubbles (PLGA-PEG-mAbCAIX NBs) to detect hypoxic breast cancer cells upon exposure of tamoxifen in nude mice. We demonstrate that carbonic anhydrase IX (CAIX) expression is associated with breast cancer local recurrence and tamoxifen resistance both in clinical and cellular models. We find that CAIX overexpression increases tamoxifen tolerance in MCF-7 cells and predicts early tamoxifen resistance along with an oscillating pattern in intracellular ATP level in vitro. PLGA-PEG-mAbCAIX NBs are able to dynamically detect tamoxifen-induced hypoxia and tamoxifen resistance in vivo. CAIX-conjugated NBs with noninvasive ultrasound imaging is powerful for dynamically monitoring hypoxic microenvironment in ER+ breast cancer with tamoxifen resistance.

12.
Artigo em Inglês | MEDLINE | ID: mdl-31909861

RESUMO

OBJECTIVE: This study sought to assess whether the predilation, scaffold/stent sizing, and postdilation (PSP) score for bioresorbable scaffold (BRS) implantation was associated with outcomes following metallic drug-eluting stent (DES) implantation. BACKGROUND: The PSP score is associated with patients' prognoses after BRS implantation. METHODS: This study involved 2,348 patients who underwent biodegradable polymer DES implantations during the PANDA III trial. The optimal PSP technique was defined according to previous studies of BRS implantations. The main outcome was target lesion failure (TLF) that comprised cardiac death, target-vessel myocardial infarction, or ischemia-driven target lesion revascularization. RESULTS: Twenty-five (1.1%) patients fulfilled all the PSP criteria. The BRS-derived PSP score was of limited prognostic value for 2-year TLF after metallic DES implantation; optimal sizing was a protective factor, but optimal predilation was a risk factor. We built a new PSP model for DESs by identifying the following risk factors: predilation performed with a residual stenosis ≥70% or a balloon-to-quantitative coronary angiography (QCA)-determined reference vessel diameter (RVD) ratio >1:1, sizing performed with an RVD <2.25 mm or a stent diameter >0.25 mm wider than the QCA-RVD, a postprocedural stenosis diameter ≥30%, age, and the baseline SYNergy between percutaneous coronary intervention with TAXus and cardiac surgery (SYNTAX) score. The new PSP scoring system predicted 2-year TLF (area under the curve, 0.69; 95% confidence interval, 0.65-0.73); a cutoff value of 19.2 points identified high-risk patients. CONCLUSIONS: The new PSP scoring system, based on redefined PSP criteria, age, and the SYNTAX score, could help optimize metallic DES implantations.

13.
Artigo em Inglês | MEDLINE | ID: mdl-31909884

RESUMO

OBJECTIVES: The FUTURE-I study aimed to assess preliminary safety and effectiveness with the long-term clinical and imaging follow-up for the Firesorb (MicroPort, Shanghai, China), a thinner-strut sirolimus-eluting bioresorbable scaffold (BRS). BACKGROUND: First-generation BRS has been associated with unexpected device-related adverse outcomes at long-term follow-up. METHODS: In this prospective, open-label, first-in-man study, patients with single de novo lesions in native coronary arteries were randomized 2:1 into two cohorts after successful Firesorb implantation: cohort 1 (n = 30) underwent multimodality imaging assessment at 6 and 24 months; and cohort 2 (n = 15) at 12 and 36 months. All patients underwent clinical follow-up at 1, 6, and 12 months and annually up to 5 years. RESULTS: Between January and March 2016, 45 patients were enrolled. At 3-year follow-up, one patient had experienced target lesion failure and none scaffold thrombosis. In-scaffold minimal lumen diameter decreased significantly from 6-month to 2-year (2.53 ± 0.24 mm vs. 2.27 ± 0.37 mm, p = .0003), and only numerically from 1-year to 3-year follow-up (2.48 ± 0.28 mm vs. 2.22 ± 0.13 mm, p = .08). By optical coherence tomography, neointimal strut coverage at 3-year follow-up was 99.8%, and very low rate of late scaffold discontinuity was observed, only in one patient on two cross sections with three malapposed struts. CONCLUSIONS: At 3-year follow-up of the FUTURE-I study, implantation of the thinner-strut Firesorb BRS appeared preliminary feasible and effective in the treatment of patients with noncomplex coronary lesions.

14.
Sci Total Environ ; 705: 135854, 2020 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-31972921

RESUMO

The disposal of ladle furnace slag (ladle slag, LS) containing traces of heavy metals produced during steelmaking has become an environmental issue. The use of LS as a binding material in civil engineering is a potential solution. In this context, this study firstly attempted to activate LS with sodium hydroxide (NaOH), sodium sulfate (Na2SO4), and sodium metasilicate (Na2SiO3), and then blended it with ground granulated blast-furnace slag (GGBS) with different LS:GGBS ratios. The chemical-activated LS pastes and LS-GGBS pastes were cured for different ages, and then subjected to a compressive strength test. The results indicated NaOH, Na2SO4, and Na2SiO3 could not effectively activate this LS, with 28-day strength <2 MPa, whilst the LS-GGBS yielded much higher strength, up to 15.6 MPa at 28 days. Only a very low concentration of Pb leached out from the LS-GGBS at 14 days, and none of the possible heavy metals were detected at 56 days. This indicates that LS-GGBS can be potentially used as a binding material in civil engineering. The X-ray diffraction (XRD) revealed that the Ca(OH)2 in LS acted as the main activator for GGBS hydration; the MgO and CaCO3 in LS seemed to play similar roles as that of the Ca(OH)2. The XRD, thermogravimetric analysis (TGA), fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FESEM), and energy dispersive X-ray spectroscopy (EDX) indicated that the main hydration product of LS-GGBS was calcium silicate hydrates (CSH).

15.
Chem Commun (Camb) ; 56(10): 1589-1592, 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-31934696

RESUMO

Single crystals of Spiro(TFSI)2 were grown, the optical and electronic properties were characterized and compared with neutral Spiro-OMeTAD. Density-functional theory was used to get insights into binding and band structure properties. The flat valence bands indicate a rather limited orbital overlap in Spiro(TFSI)2.

16.
Biochem Biophys Res Commun ; 523(3): 699-706, 2020 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-31948749

RESUMO

The WD repeat domain 5 (WDR5), also known as SWD3 and BIG-3, is often overexpressed in cancers, however its molecular function in cancer remains to be elucidated. In this study, we found that WDR5 promoted the proliferation and self-renewal of glioblastoma and neuroblastoma cells. The data from databases and Western blot assay showed that CARM1 is a downstream gene of WDR5-Myc axis. In addition, we observed that WDR5 promoted the binding of Myc to CARM1 promoter by interacting with Myc and inducing histone 3 lysine 4 trimethylation (H3K4me3). Dual luciferase reporter system indicated that Myc binds to the upstream region (-520 to -515) before transcription start site (TSS) of CARM1 promoter. These findings suggest a novel regulatory model for the proliferation and tumorigenesis of glioblastoma and neuroblastoma by WDR5-Myc axis.

17.
Artigo em Inglês | MEDLINE | ID: mdl-31943685

RESUMO

OBJECTIVES: This study aimed to evaluate the safety and efficiency of percutaneous transluminal angioplasty (PTA) with selective stenting treating Chinese patients with renal artery stenosis (RAS) due to fibromuscular dysplasia (FMD). BACKGROUND: Endovascular data of patients with RAS caused by FMD are scarce in China. METHODS: Clinical data of 105 hypertensive patients with RAS caused by FMD underwent endovascular therapy at a single-site between June 2001 and October 2018 were analyzed retrospectively. Baseline blood pressure, use of antihypertensive medications, renal function, and adverse events at a 1-year follow-up were evaluated. RESULTS: The patients were aged between 10 and 64 years (mean age 26.7 ± 8.2 years) and 52 (49.5%) were female. In total, 105 patients undergoing endovascular therapy involving 124 RASs. Thirty (24.2%), sixty-four (51.6%), and thirty (24.2%) RASs were multifocal, unifocal, and tubular types, respectively. Ninety-four (89.5%) and eleven (10.5%) patients underwent PTA alone and PTA plus stenting, respectively. The technical success rate for endovascular therapy was 95.2% (100/105) without severe complications. During 1-year follow-up (n = 100), the mean systolic and diastolic blood pressure decreased from 157.6 ± 17.5 and 102.3 ± 14.2 to 129.6 ± 12.3 and 81.3 ± 11.1 mmHg, respectively, and the number of antihypertensive medications reduced from 2.2 ± 1.2 to 0.8 ± 1.0 (all p < .001). The cure rate and improved rate of blood pressure was 49.0 and 40.0%, respectively. The serum creatinine levels remained stable. The primary and secondary restenosis rate was 13.4 and 5.8%, respectively. CONCLUSION: Endovascular therapy for treating RAS caused by FMD was technically safe and effective for reducing blood pressure in Chinese patients.

18.
Artigo em Inglês | MEDLINE | ID: mdl-31943693

RESUMO

OBJECTIVES: To report the clinical outcomes of the RESTORE drug-coated balloon (DCB; Cardionovum, Bonn, Germany) for treatment of de novo small vessel disease (SVD) beyond 1 year. BACKGROUND: Previous reports have demonstrated the noninferiority of the RESTORE DCB to the RESOLUTE Integrity drug-eluting stent (DES; Medtronic, Minneapolis, Minnesota) in terms of 9-month in-segment percent diameter stenosis. METHODS: In the prospective, multicenter, noninferiority RESTORE SVD China trial, 230 patients with visually-estimated reference vessel diameter (RVD) ≥2.25 and ≤2.75 mm were randomized to DCB or DES in a 1:1 ratio stratified by diabetes and number of lesions treated. Furthermore, 32 patients with RVD ≥2.00 and <2.25 mm were enrolled in a nested very small vessel (VSV) registry. Clinical follow-up were performed at 2 years to evaluate target lesion failure (TLF) in both groups and the VSV cohort. RESULTS: Overall, 256 (97.7%) patients (115 and 109 in the DCB and DES groups, respectively, and 32 in the VSV cohort) completed 2 years of follow-up. There was no significant difference in TLF between the DCB and DES groups (5.2 vs. 3.7%, p = .75). Target lesion revascularization was acceptable at 1 month, 1 year, and 2 years, and did not differ significantly with DCB from that in the DES group (0.9 vs. 0%, p = 1.0, 4.4 vs. 2.6%, p = .72, 5.2 vs. 2.8%, p = .50, respectively). CONCLUSIONS: Compared to the second-generation DES, the RESTORE DCB did not increase the risk of clinical outcomes. Late catch-up phenomen requiring revascularization was not significant in this study.

19.
ChemSusChem ; 2020 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-31965761

RESUMO

Great efforts have been made to understand and upgrade the kinetically sluggish oxygen evolution reaction (OER). Herein, a series of V doped LaCoO 3  (V-LCO) OER electrocatalysts with optimized d band center are successfully fabricated. When utilized as electrode for the OER, as-formed V-LCO-II demands overpotential of only 306 mV to drive a geometrical catalytic current density of 10 mA cm -2 . Furthermore, at a given overpotential of 350 mV, the OER current density of V-LCO-II is about 22 times that of pure LCO nanoparticles. The V dopant tailored d-band center facilitate the adsorption of OER intermediates, and promote the formation of amorphous active species on the surface of LCO due to the notable exchange interaction between high-spin V 4 +  and low-spin Co 2+ . This work may create new opportunities for developing other high active OER catalysts via d band centers engineering.

20.
J Craniofac Surg ; 2020 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-31977712

RESUMO

PURPOSE: To compare the clinical efficacy of disposable multifunctional drainage tube (DMDT)-assisted irrigation with the traditional abscess incision rubber drainage technique in patients with severe multi-space infections in oral and maxillofacial head and neck regions. PATIENTS AND METHODS: The data of 74 patients with severe multi-space infections in oral and maxillofacial head and neck regions, who were admitted to the Department of Oral and Maxillofacial Surgery, Central Hospital of Panzhihua City, Sichuan Province, China, between January 2015 and January 2019, were retrospectively studied. According to the treatment method, the patients were divided into 2 groups: the DMDT-assisted irrigation group and the abscess incision rubber drainage group. Cure rate, complications, length of hospitalization, days of antimicrobial use, cost of antimicrobial drugs, total hospitalization cost, number of dressing changes, and patient pain during dressing changes were compared between the 2 groups. RESULTS: Of the 74 patients, 38 were treated with the DMDT-assisted irrigation, and 36 with the traditional abscess incision rubber drainage. Compared with the traditional treatment group, the total hospitalization cost of the DMDT-assisted irrigation group is not much different (P = 0.72), but the patients in the DMDT-assisted irrigation group have higher cure rate, fewer complications, less antibiotics cost, shorter hospitalization length and fewer dressing changes than the control group (P < 0.05). CONCLUSIONS: The DMDT-assisted irrigation technique not only improves the cure rate, reduces complications, shortens hospitalization, reduces the use of antibiotics, and alleviates patient pain during dressing changes, but also improves clinical and therapeutic efficacy by reducing the number of dressing changes.

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