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1.
PLoS One ; 16(1): e0244978, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33406133

RESUMO

The location-based services can provide users with the requested location information. But users also need to disclose their current location to the location-based service provider. Therefore, how to protect user's location privacy is a major concern. In this paper, we propose a heterogeneous deniable authenticated encryption scheme called HDAE for location-based services. The proposed scheme permits a sender in a public key infrastructure environment to transmit a message to a receiver in an identity-based environment. Our design utilizes a hybrid encryption method combing the tag-key encapsulation mechanism (tag-KEM) and the data encapsulation mechanism (DEM), which is well adopted for location-based services applications. We give how to design an HDAE scheme utilizing a heterogeneous deniable authenticated tag-KEM (HDATK) and a DEM. We also construct an HDATK scheme and provide security proof in the random oracle model. Comprehensive analysis shows that our scheme is efficient and secure. In addition, we give an application of the HDAE to a location-based services system.

2.
Ann Transl Med ; 8(20): 1306, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33209886

RESUMO

Background: Monoclonal non-specific suppressor factor ß (MNSFß) is a ubiquitously expressed member of the ubiquitin-like family. It functions as a regulator of cell apoptosis and a potential tumor suppressor, playing a vital role in the processes of immune cell function and apoptosis. Methods: The present study constructed GFP-pMNSFß swine umbilical vein endothelial cell (SUVEC) lines and investigated the function of porcine MNSFß (pMNSFß) in apoptosis, as well as its interactions with pBCL-G. Results revealed that stably expressed pMNSFß protein in SUVEC lines significantly enhanced staurosporine (STS)-induced apoptosis. pMNSFß proteins interacted with pBCL-G proteins and the expression of these interacting proteins synergized to further enhance STS-induced apoptosis. Results: GFP-pMNSFß stably expressed SUVEC lines through transient transfection and neomycin screening methods. Over 90% of the SUVEC cultures expressed GFP signals, and 41.5 kDa GFP-pMNSFß proteins were detected with western blotting methods. Annexin V-PE/PI staining and flow cytometry analyses showed that overexpression of pMNSFß proteins significantly elevated STS-induced apoptosis rates. Co-immunoprecipitation methods revealed an interaction between pMNSFß and pBCL-G proteins. BCL-G is a proapoptotic member of the BCL-2 family that has been shown to be misexpressed in human systemic lupus erythematosus, as well as mammary and prostate cancers. Here, we demonstrated that the co-expression and potential conjugation of pMNSFß and pBCL-G proteins synergistically enhanced STS-induced apoptosis. Conclusions: The present study was the first to characterize the function of MNSFß in porcine cells, and to clarify the function of MNSFß in apoptosis. These results reveal that pMNSFß is a potential molecular model for future investigations of diseases related to human MNSFß dysfunction.

3.
J Craniofac Surg ; 2020 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-32897975

RESUMO

OBJECTIVE: The aim of this study is to analyze the clinical effect of small bone-window craniotomy with microscope combined postoperative ICP monitoring, and further explore an appropriate treatment for HICH patients. METHODS: One hundred fifty patients with HICH were selected according to inclusion and exclusion criteria and divided into 3 groups at random, 50 each group. Patients in 3 groups were treated with conventional craniotomy, small bone-window craniotomy and small bone-window craniotomy combined ICP monitoring respectively. The surgical efficiency, treatment effect and outcomes were recorded and analyzed. RESULTS: The intraoperative blood loss and operation time of small window groups were significantly less than that of conventional group, and the hematoma clearance rate in small window groups were significantly higher than in conventional group (P < 0.05). Compared with conventional group, the hospital stays and mannitol dose used were less in small window groups and least in small window combined ICP monitoring group (P < 0.05). The complication rate in small window combined ICP monitoring group was 10%, which was significantly lower than in conventional group (26%, P < 0.05), while no significant difference was found between small window group (18%) compared with the other 2 groups respectively (P > 0.05). The difference of morality rate between 3 groups wasn't significant (P > 0.05). Three treatment significantly increased the Barthel index score, and the improvement of small window combined ICP monitoring group was significantly higher than in other 2 groups respectively (P < 0.05), while the difference between this two groups wasn't significant (P > 0.05). CONCLUSION: Small bone-window craniotomy is more efficient and convenient than conventional craniotomy in the treatment of HICH. In the meantime, small bone-window craniotomy simultaneous with ICP monitoring significantly improved clinical effect and treatment outcomes of HICH patients.

4.
Small ; 16(42): e2004129, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32939987

RESUMO

Herein, it is demonstrated that N-rich carbonized silk fibroin materials (CSFs) can serve as efficient peroxidase, and oxidase mimics. Their enzyme-like activities are highly dependent on carbonization conditions. CSFs obtained at low temperatures do not exhibit significant catalytic reactivity, while their enzyme-like catalysis performance is greatly activated after high-temperature treatment. Such a phenomenon is mainly ascribed to the increase of graphitization degree and graphitic nitrogen and the emergence of disordered graphitic structures during the formation of turbostratic carbon. In addition, inspired by the excellent photothermal conversion efficiency, and temperature-dependent catalytic behavior of CSFs, near-infrared light can be used to remotely control their enzyme-like activities. More importantly, as-prepared robust silk-derived nanosheets can be applied to photothermal-catalytic cancer therapy and sensing. It is believed that such a smart artificial enzyme system will throw up exciting new opportunities for the chemical industry and biotechnology.

5.
Nanoscale Res Lett ; 15(1): 162, 2020 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-32780249

RESUMO

Light-activated nanozymes can provide a wealth of new opportunities for the chemical industry and biotechnology. However, present remote-controlled catalytic systems are still far from satisfactory. Herein, we present an interesting example of applying ultrathin Pd nanosheets (Pd NSs) as a light-controllable peroxidase mimic. Since most of Pd atoms are exposed on their surface, Pd NSs with a thickness of 1.1 nm possess high peroxidase-like activity. More importantly, under light excitation, such intrinsic activity can be further activated by a nearly 2.4- to 3.2-fold. Such a phenomenon can be ascribed to the unique optical property of ultrathin Pd NSs, which can efficiently capture photons to generate hot electrons via surface plasmon resonance effect and thus promote the in situ decomposition of H2O2 into reactive oxygen species radicals (O*). This enhanced catalysis can also be used for real-time and highly sensitive colorimetric detection of H2O2. We expect our work can provide valuable insights into the rational design of artificial nanozymes with controllable and efficient activity in biomedical diagnostics, drug delivery, and environmental chemistry.

6.
J Evid Based Med ; 11(2): 101-104, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29761647

RESUMO

OBJECTIVE: IL-22 plays an important role in cancer carcinogenesis. However, the association between IL-22 polymorphisms and cancer risk was inconclusive. The objective of the current study was to assess these associations by meta-analysis. METHODS: PubMed, EMbase, CNKI, VIP, and Wanfang databases were searched up to 31 January 2018. The results were screened according to the inclusion and exclusion criteria. The associations between polymorphisms and cancer risk were estimated by meta-analysis. All analyses were performed using the Revman5.3 software. RESULTS: A total of four polymorphisms (rs2227485, rs1179251, rs1179246, and rs1182844) in seven studies were included. The results of meta-analysis indicated that the rs1179251 polymorphism [OR = 1.46, 95% CI (1.17, 1.82), P = 0.0008 for GG+GC vs. CC] was associated with increased risk of cancer, while the rs2227485, rs1179246, and rs1182844 polymorphisms were not associated with cancer risk. CONCLUSIONS: The current meta-analysis suggests that IL-22 gene rs1179251 polymorphism may be a risk factor for cancer.


Assuntos
Predisposição Genética para Doença , Interleucinas/genética , Neoplasias/genética , Humanos , Polimorfismo de Nucleotídeo Único
7.
J Evid Based Med ; 10(3): 207-211, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28857506

RESUMO

BACKGROUND: Genetic susceptibility of lung cancer has been widely studied for Chinese population, and meta-analysis of candidate gene association studies has also been performed for those genes. However, the overall evidence has not been well recognized. OBJECTIVE: To investigate genetic association for the risk of lung cancer in Chinese. METHOD: An overview of systematic reviews and meta-analyses of candidate gene association studies for lung cancer in Chinese was performed up to August 10th , 2016. The AMSTAR tool was used to assess the quality of the included systematic reviews and meta-analyses. Bibliometric analysis was performed to analyze the characteristics of reviews. RESULTS: A total of 21 variants in 17 genes from 20 meta-analyses were included in this study. All 20 meta-analyses were published from 2011 to 2016. The quality scores of AMSTAR ranged from 3 to 7. All included genes were in the pathogenesis of lung cancer, such as the CYPs genes, GSTs genes, and base excision repair genes. Three polymorphisms were found to be associated with decreased risk of lung cancer for Chinese, 15 polymorphisms were found to be associated with increased risk of lung cancer for Chinese, but three polymorphisms were found to be not associated with lung cancer risk for Chinese. CONCLUSION: The current study supports the genetic risk factors of lung cancer for Chinese are more likely to be variants from genes that contribute to the etiology of lung cancer.


Assuntos
Predisposição Genética para Doença , Neoplasias Pulmonares/genética , Polimorfismo Genético , China , Estudo de Associação Genômica Ampla , Humanos , Neoplasias Pulmonares/etnologia , Literatura de Revisão como Assunto
8.
Medicine (Baltimore) ; 95(40): e4423, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27749524

RESUMO

BACKGROUND: The effects of the programed cell death 1 (PDCD1) gene polymorphisms on cancer risk have been investigated in some studies; however, the results were conflicting and ambiguous. Therefore, we aimed to do a meta-analysis to investigate the association of PDCD1 polymorphisms with cancer risk from all eligible case-control studies. MATERIALS AND METHODS: An electronic search of the PubMed, Embase, Chinese National Knowledge Infrastructure, and Wanfang databases was performed. The association between PDCD1 polymorphisms with cancer risk was calculated with odds ratios (ORs) and their corresponding 95% of confidence intervals (CIs). RESULTS: A total of 24 case-control studies from 13 articles that investigated the associations of 5 widely studied polymorphisms in PDCD1 gene and cancer risks were included. The results of meta-analysis: the PDCD-1.5 (rs2227981) and PDCD-1.3 (rs11568821) polymorphisms were associated with decreased risk of cancer (rs2227981: OR = 0.75, 95% CI: 0.64-0.86, P < 0.0001 for TT vs TC + CC; rs11568821: OR = 0.79, 95% CI: 0.65-0.96, P = 0.02 for TC vs TT), while no significant associations were found for the other 3 polymorphisms (PDCD-1.9 [rs2227982] polymorphism: OR = 1.03, 95% CI: 0.90-1.18, P = 0.66 for CC + TC vs TT; PDCD1 rs7421861 polymorphism: OR = 1.10, 95% CI: 0.96-1.25, P = 0.16 for CC + TC vs TT; PDCD-1.6 [rs10204525] polymorphism: OR = 0.93, 95% CI: 0.82-1.05, P = 0.24 for GG + GA vs AA). CONCLUSION: The meta-analysis suggests that the PDCD-1.5 (rs2227981) and PDCD-1.3 (rs11568821) polymorphisms are associated with susceptibility of cancer. Further studies with larger sample sizes are required to make a better assessment of the above association.


Assuntos
Polimorfismo Genético/genética , Receptor de Morte Celular Programada 1/genética , Estudos de Casos e Controles , Predisposição Genética para Doença , Humanos
9.
Zhonghua Xin Xue Guan Bing Za Zhi ; 41(5): 406-10, 2013 May.
Artigo em Chinês | MEDLINE | ID: mdl-24021124

RESUMO

OBJECTIVE: To explore the role and potential mechanism of human α-defensin 1 (HNP-1) on low-density lipoprotein (LDL) oxidation ability of human endothelial cells (EVC304). METHODS: Post incubation with LDL for 3 h, the malondialdehyde (MDA) and protein carbonyl (PCO) were detected in untreated ECV304 (control) and in HNP-1 transfected ECV304 in the presence and absence of siRNA against HNP-1. Flow cytometry and fluorescence microscopy were used to detect the generation of oxygen free radical in the ECV304 which have been pretreated by LDL, LPS and HNP-1, respectively. RESULT: Compared with control group, MDA level was significantly increased in HNP-1 transfected [(4.21 ± 0.03) vs. (3.15 ± 0.02) nmol/mg · pro] or in HNP-1 stimulated ECV304 cells [(14.49 ± 1.10) vs. (9.47 ± 1.18) nmol/mg · pro], which could be significantly downregulated by siRNA [(3.76 ± 0.48) vs. (4.54 ± 0.28) nmol/mg·pro, all P < 0.05]. PCO was also significantly increased in HNP-1 transfected ECV304 cells. The levels of free radical were significantly increased in HNP-1 transfected or HNP-1 stimulated ECV304 cells. CONCLUSION: HNP-1 can enhance the LDL oxidation ability of human endothelial cells via promoting the generation of free radicals.


Assuntos
Células Endoteliais/metabolismo , Lipoproteínas LDL/metabolismo , alfa-Defensinas/genética , Linhagem Celular , Humanos , RNA Interferente Pequeno , Transfecção , alfa-Defensinas/metabolismo
11.
Chaos ; 23(1): 013140, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23556977

RESUMO

In this paper, a novel kind of compound synchronization among four chaotic systems is investigated, where the drive systems have been conceptually divided into two categories: scaling drive systems and base drive systems. Firstly, a sufficient condition is obtained to ensure compound synchronization among four memristor chaotic oscillator systems based on the adaptive technique. Secondly, a secure communication scheme via adaptive compound synchronization of four memristor chaotic oscillator systems is presented. The corresponding theoretical proofs and numerical simulations are given to demonstrate the validity and feasibility of the proposed control technique. The unpredictability of scaling drive systems can additionally enhance the security of communication. The transmitted signals can be split into several parts loaded in the drive systems to improve the reliability of communication.


Assuntos
Comunicação , Eletrônica/instrumentação , Dinâmica não Linear , Medidas de Segurança , Teoria de Sistemas , Simulação por Computador , Desenho de Equipamento , Estudos de Viabilidade , Análise Numérica Assistida por Computador , Oscilometria , Processamento de Sinais Assistido por Computador , Fatores de Tempo
12.
Clin Chim Acta ; 418: 79-85, 2013 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-23305798

RESUMO

BACKGROUND: Leukocyte number in the circulation plays a central role in inflammatory diseases, such as coronary heart disease (CHD). Increased counts are correlated with the intensity of the peri-infarction inflammatory response and adverse outcomes. We investigated leukocyte and leukocyte subset counts in dyslipidaemia patients and their relationship with LDL oxidation. METHODS: Dyslipidaemia patients (207) were selected for blood counts and LDL-C testing. The level of HNP-1and myeloperoxidase in subsets of leukocytes and their relationship with LDL oxidation were compared between 24 CHD patients and 24 normal controls. RESULTS: In dyslipidaemia patients, total leukocyte and neutrophil counts increased with LDL-C (p=0.001). Monocyte counts showed the opposite trend (p=0.001). Although serum HNP-1 levels were not different between CHD patients and normal controls (p=0.558), neutrophil HNP-1 mRNA levels were 2.13-fold greater than those of normal controls. However, monocyte HNP-1 mRNA levels were lower (p=0.005). The distribution of myeloperoxidase in monocytes and neutrophils is different, myeloperoxidase locates mainly in the cytoplasm of monocytes, on the cell membrane of neutrophils. CONCLUSIONS: Leukocyte and leukocyte subset counts may correlate with LDL-C levels and LDL oxidation. The monocyte-neutrophil interaction reveals a potential compensatory mechanism associated with LDL oxidation in CHD that may be a prognostic factor of CHD.


Assuntos
Doença das Coronárias/patologia , Leucócitos/patologia , Células Cultivadas , LDL-Colesterol/metabolismo , Doença das Coronárias/sangue , Doença das Coronárias/metabolismo , Dislipidemias/sangue , Dislipidemias/metabolismo , Dislipidemias/patologia , Feminino , Humanos , Contagem de Leucócitos , Leucócitos/metabolismo , Masculino , Pessoa de Meia-Idade , Oxirredução , Peroxidase/metabolismo , RNA Mensageiro/sangue , RNA Mensageiro/genética , alfa-Defensinas/sangue , alfa-Defensinas/genética
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