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1.
BMC Plant Biol ; 22(1): 12, 2022 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-34979929

RESUMO

BACKGROUND: Taxol from Taxus species is a precious drug used for the treatment of cancer and can effectively inhibit the proliferation of cancer cells. However, the growth of Taxus plants is very slow and the content of taxol is quite low. Therefore, it is of great significance to improve the yield of taxol by modern biotechnology without destroying the wild forest resources. Endophytic fungus which symbiosis with their host plants can promote the growth and secondary metabolism of medicinal plants. RESULTS: Here, an endophytic fungus KL27 was isolated from T. chinensis, and identified as Pseudodidymocyrtis lobariellae. The fermentation broth of KL27 (KL27-FB) could significantly promote the accumulation of taxol in needles of T. chinensis, reaching 0.361 ± 0.082 mg/g·DW (dry weight) at 7 days after KL27-FB treatment, which is 3.26-fold increase as compared to the control. The RNA-seq and qRT-PCR showed that KL27-FB could significantly increase the expression of key genes involved in the upstream pathway of terpene synthesis (such as DXS and DXR) and those in the taxol biosynthesis pathway (such as GGPPS, TS, T5OH, TAT, T10OH, T14OH, T2OH, TBT, DBAT and PAM), especially at the early stage of the stimulation. Moreover, the activation of jasmonic acid (JA) biosynthesis and JA signal transduction, and its crosstalk with other hormones, such as gibberellin acid (GA), ethylene (ET) and salicylic acid (SA), explained the elevation of most of the differential expressed genes related to taxol biosynthesis pathway. Moreover, TF (transcriptional factor)-encoding genes, including MYBs, ethylene-responsive transcription factors (ERFs) and basic/helix-loop-helix (bHLH), were detected as differential expressed genes after KL27-FB treatment, further suggested that the regulation of hormone signaling on genes of taxol biosynthesis was mediated by TFs. CONCLUSIONS: Our results indicated that fermentation broth of endophytic fungus KL27-FB could effectively enhance the accumulation of taxol in T. chinensis needles by regulating the phytohormone metabolism and signal transduction and further up-regulating the expression of multiple key genes involved in taxol biosynthesis. This study provides new insight into the regulatory mechanism of how endophytic fungus promotes the production and accumulation of taxol in Taxus sp.


Assuntos
Ascomicetos/fisiologia , Endófitos/fisiologia , Regulação da Expressão Gênica de Plantas , Paclitaxel/biossíntese , Reguladores de Crescimento de Plantas/metabolismo , Transdução de Sinais , Taxus/metabolismo , Genes de Plantas , Paclitaxel/metabolismo , Taxus/microbiologia , Regulação para Cima
2.
J Eukaryot Microbiol ; : e12878, 2021 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-34877732

RESUMO

To find out whether and how the prevalence and genetic diversity of Cryptosporidium in neonatal calves varies with the season, 380 fecal samples from neonatal calves on two large-scale farms in Xinjiang (Alar and Wensu) were studied using molecular biology techniques. Cryptosporidium was detected in 48.7% (185/380) of the samples, and was most frequent in summer (56.8%), followed by spring (50.0%), winter (46.8%), and autumn (41.7%) (P > 0.05). Calves with diarrhea seem to be more likely infected by Cryptosporidium than those without diarrhea (P < 0.01). We also found that C. parvum (n = 173), C. bovis (n = 7) and C. ryanae (n = 3), were the Cryptosporidium species detected in this study, and coinfections of these three species (n = 2) were also identified. Two subtypes (IIdA14G1 and IIdA15G1) of C. parvum were identified and both can infect human. These results also show that neonatal calves commonly suffer diarrhea caused by C. parvum throughout the year.

4.
Res Vet Sci ; 141: 138-144, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34740045

RESUMO

Enterocytozoon bieneusi is a common gastrointestinal parasite that has a broad range of hosts, including birds, mammals, and even humans. The changes of gut bacterial communities have been demonstrated during the course of E. bieneusi. This study aimed to examine the infection status, and assess the zoonotic potential and influence of E. bieneusi on gut bacterial communities of captive foxes in Xinxiang, central China. Fecal samples were collected from 216 captive foxes, and amplification of the internal transcribed spacer (ITS) gene of each sample was performed by nested polymerase chain reaction. E. bieneusi was detected in 48 (22.2%) samples, and five previously reported genotypes CHN-F1 (n = 25), D (n = 18), BEB6 (n = 3), NCF2 (n = 1), and CHN-DC1 (n = 1) were identified, with CHN-F1 being the dominant genotype (25/48, 52.1%). Phylogenetic analysis further revealed that CHN-F1, D, NCF2, and CHN-DC1 genotypes belonged to the zoonotic group 1 of E. bieneusi, whereas BEB6 belonged to group 2. Moreover, sequencing and bioinformatics analysis of the V3-V4 region of 16S rRNA gene in five E. bieneusi-positive and five negative samples showed that gut microbial diversity was higher in the infected animals. In both groups, Firmicutes and Bacteroidetes were the two most abundant phyla, but the Firmicutes/Bacteroidetes ratio was lower in E. bieneusi-positive foxes (7.9:1) as compared with E. bieneusi-negative foxes (5:1). More importantly, at the phylum level, "beneficial bacteria" such as Firmicutes (1.6%) and Bacteroides (5.8%) increased, whereas "opportunistic pathogens" such as Fusobacteria (8.2%) decreased. Similarly, at the genus level, the pathogenic Clostridiaceae_Clostridium (10.0%) decreased, whereas the "beneficial bacteria" Lactococcus (6.6%) increased. These interesting phenomena warrant further investigation.

5.
Microbiol Spectr ; : e0123121, 2021 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-34730415

RESUMO

The spread of multidrug-resistant (MDR) Gram-negative bacteria (GNB) has led to serious public health problems worldwide. Colistin, as a "last resort" for the treatment of MDR bacterial infections, has been used significantly in recent years and has led to the continuous emergence of colistin-resistant strains. In this study, we aimed to investigate the synergistic effect on the antimicrobial and antibiofilm activities of a colistin/furanone C-30 combination against colistin-resistant GNB in vitro and in vivo. According to antimicrobial resistance profiles, most of the colistin-resistant strains we collected showed MDR phenotypes. The checkerboard method and time-kill curve showed that the combination with furanone C-30 increases the antibacterial activity of colistin significantly. In addition, the furanone C-30/colistin combination can not only inhibit the formation of bacterial biofilm but also has a better eradication effect on preformed mature biofilms. The result of scanning electron microscopy (SEM) demonstrated that the furanone C-30/colistin combination led to a significant reduction in the number of cells in biofilms. Furthermore, furanone C-30 at 50 µg/ml did not cause any additional toxicity to RAW264.7 cells according to a cytotoxicity assay. In in vivo infection experiments, the furanone C-30/colistin combination increased the survival rate of infected Galleria mellonella larvae as well as decreased the microbial load in a mouse thigh infection model. The synergistic effect of the furanone C-30/colistin combination against colistin-resistant GNB is encouraging, and this work may shed light on a new therapeutic approach to combat colistin-resistant pathogens. IMPORTANCE Colistin is among the few antibiotics effective against multidrug-resistant Gram-negative bacteria (GNB) clinical isolates. However, colistin-resistant GNB strains have emerged in recent years. Therefore, the combination of colistin and nonantibacterial drugs has attracted much attention. In this study, the furanone C-30/colistin combination showed good antibacterial and antibiofilm activity in vitro and in vivo. In addition, increased membrane permeability leads to the synergistic effect of the furanone C-30/colistin combination. Because of the low cytotoxicity of furanone C-30, this combination has good application prospects in clinical anti-infective therapy. This finding might shed light on the discovery of combination therapy for infections caused by colistin-resistant GNB pathogens.

6.
Front Med (Lausanne) ; 8: 696976, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34604251

RESUMO

Background: Previous research suggested that Chinese Medicine (CM) Formula Huashibaidu granule might shorten the disease course in coronavirus disease 2019 (COVID-19) patients. This research aimed to investigate the early treatment effect of Huashibaidu granule in well-managed patients with mild COVID-19. Methods: An unblinded cluster-randomized clinical trial was conducted at the Dongxihu FangCang hospital. Two cabins were randomly allocated to a CM or control group, with 204 mild COVID-19 participants in each cabin. All participants received conventional treatment over a 7 day period, while the ones in CM group were additionally given Huashibaidu granule 10 g twice daily. Participants were followed up to their clinical endpoint. The primary outcome was worsening symptoms before the clinical endpoint. The secondary outcomes were cure and discharge before the clinical endpoint and alleviation of composite symptoms after the 7 days of treatment. Results: All 408 participants were followed up to their clinical endpoint and included in statistical analysis. Baseline characteristics were comparable between the two groups (P > 0.05). The number of worsening patients in the CM group was 5 (2.5%), and that in the control group was 16 (7.8%) with a significant difference between groups (P = 0.014). Eight foreseeable mild adverse events occurred without statistical difference between groups (P = 0.151). Conclusion: Seven days of early treatment with Huashibaidu granule reduced the likelihood of worsening symptoms in patients with mild COVID-19. Our study supports Huashibaidu granule as an active option for early treatment of mild COVID-19 in similar well-managed medical environments. Clinical Trial Registration:www.chictr.org.cn/showproj.aspx?proj=49408, identifier: ChiCTR2000029763.

7.
J Clin Ultrasound ; 2021 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-34676544

RESUMO

We used cardiac POCUS to capture a thrombus through and straddling PFO and determined its peripheral venous source. Complete dissolution of large trapped thrombus and remission of signs of pulmonary embolism was monitored in time, which was of great value for subsequent treatment and decision-making.

8.
Am J Transl Res ; 13(8): 9679-9686, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34540095

RESUMO

OBJECTIVE: To explore the effects of bundle nursing on PICC-related upper extremity deep venous thrombosis (PICC-UEDVT) in patients with non-Hodgkin's lymphoma (NHL) who are undergoing chemotherapy, and contributing factors toward PICC-UEDVT. METHODS: 370 NHL patients undergoing chemotherapy in the Department of Hematology were selected prospectively and randomized into a control group (receiving routine nursing) and an observation group (receiving bundle nursing) using random number method. There were 185 patients in each group. The incidence of PICC-UEDVT and satisfaction rate towards nursing were calculated and risk factors for PICC-UEDVT were explored in the two groups. RESULTS: The incidence of PICC-UEDVT was lower in the observation group (18 patients, 9.73%) than that of the control group (35 patients, 18.92%; P<0.05); the satisfaction rate towards nursing was higher in the observation group than the control group (P<0.05). Tumor stage, ECOG score >2, hemoglobin <100 g/L, and D-dimer >0.5 mg/L were factors that contributed to the occurrence of PICC-UEDVT in NHL patients (P<0.05). CONCLUSION: Tumor stage, ECOG score >2, reduced hemoglobin, and increased D-dimer levels are predisposing factors for PICC-UEDVT in NHL patients. However, bundle nursing can reduce the incidence of PICC-UEDVT and increase the satisfaction rate towards nursing.

9.
Front Psychol ; 12: 732445, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34531802

RESUMO

Psychological capital (PsyCap) is documented to be positive in influencing employees' behavior. However, little attention has been paid to its role in maintaining a sustainable workforce in underprivileged rural areas. Also less known is the complex relations between PsyCap and other consequence variables. Moreover, previous studies in this field did not adequately address the cross-cultural applications of positive resources, though many facets of PsyCap are culture related. To address the gaps, the current study explored the complex relationships linking PsyCap and organizational commitment in a sample of public civil servants (gongwuyuan cadres, n=583) at the township level in the rural areas of northwestern China. Two types of PsyCap, task-oriented PsyCap, which is similar to the PsyCap in the west, and guanxi-oriented PsyCap, which is unique in the Chinese culture, were measured. Task-oriented PsyCap is composed of enterprise-diligence, resiliency-perseverance, optimism-hope, and confidence-courage. Guanxi-oriented PsyCap is composed of toleration-forgiveness, modesty-prudence, thanksgiving-dedication, and respect-courtesy. AMOS 23.0 software was used to establish structural equation models. The results show that both types of PsyCap were positive predictors of organizational commitment. Vocational identity and job satisfaction mediated the relation between task-oriented PsyCap/guanxi-oriented PsyCap and organizational commitment. The chained relationship from the two types of PsyCap to vocational identity, job satisfaction, and organizational commitment was also significant. These results and their implications for workforce stability are discussed.

10.
Phytomedicine ; 91: 153671, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34425471

RESUMO

OBJECTIVE: To evaluate the efficacy and safety of Hua Shi Bai Du Granule (Q-14) plus standard care compared with standard care alone in adults with coronavirus disease (COVID-19). STUDY DESIGN: A single-center, open-label, randomized controlled trial. SETTING: Wuhan Jinyintan Hospital, Wuhan, China, February 27 to March 27, 2020. PARTICIPANTS: A total of 204 patients with laboratory-confirmed COVID-19 were randomized into the treatment group and control group, consisting of 102 patients in each group. INTERVENTIONS: In the treatment group, Q-14 was administered at 10 g (granules) twice daily for 14 days, plus standard care. In the control group, patients were provided standard care alone for 14 days. MAIN OUTCOME MEASURE: The primary outcome was the conversion time for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral assay. Adverse events were analyzed in the safety population. RESULTS: Among the 204 patients, 195 were analyzed according to the intention-to-treat principle. A total of 149 patients (71 vs. 78 in the treatment and control groups, respectively) tested negative via the SARS-CoV-2 viral assay. There was no statistical significance in the conversion time between the treatment group and control group (Full analysis set: Median [interquartile range]: 10.00 [9.00-11.00] vs. 10.00 [9.00-11.00]; Mean rank: 67.92 vs. 81.44; P = 0.051). The recovery time for fever was shorter in the treatment group than in the control group. The disappearance rate of symptoms like cough, fatigue, and chest discomfort was significantly higher in the treatment group. In chest computed tomography (CT) examinations, the overall evaluation of chest CT examination after treatment compared with baseline showed that more patients improved in the treatment group. There were no significant differences in the other outcomes. CONCLUSION: The combination of Q-14 and standard care for COVID-19 was useful for the improvement of symptoms (such as fever, cough, fatigue, and chest discomfort), but did not result in a significantly higher probability of negative conversion in the SARS-CoV-2 viral assay. No serious adverse events were observed. TRIAL REGISTRATION: ChiCTR2000030288.


Assuntos
COVID-19 , Medicamentos de Ervas Chinesas/uso terapêutico , COVID-19/terapia , China , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Resultado do Tratamento
11.
Artigo em Inglês | MEDLINE | ID: mdl-34197932

RESUMO

OBJECTIVES: China banned the use of colistin as animal growth promoter in April 2017. Herein, we report the prevalence of mcr-1 in the intestine of healthy humans and risk factors associated with mcr-1 carriage after the implementation of the ban. METHODS: We recruited 719 healthy volunteers from Shenzhen City from 1 March 2018 to 31 December 2019 to investigate the prevalence of mcr-1 in human intestine, and undertook a case-control study to ascertain the risk factors associated with the mcr-1-positive population. A further comparative study was conducted to identify differences between genetic characteristics of mcr-1-positive and mcr-1-negative Escherichia coli. RESULTS: Overall, 56 (7.8%, 95% CI 5.9%-10.0%, n = 719) individual faecal samples were positive for mcr-1, and prevalence of mcr-1 among individuals in 2019 (2.4%, 95% CI 8.7%-15.0%, 7/294) was significantly lower than that in 2018 (11.5%, 95% CI 1.0%-4.8%, 49/425) (p < 0.0001). After the colistin ban, animal-derived food (pork and chicken meat) was no longer a risk factor for mcr-1 carriage in human intestine, whereas a higher intake of fish and seafood (>75 g/day) and whole grains (>150 g/day) was associated with higher and lower risk of mcr-1 carriage, respectively (OR 2.175, 95% CI 1.047-4.517; OR 0.045, 95% CI 0.004-0.567). Compared with mcr-1-negative E. coli, the mcr-1-positive E. coli had different patterns of resistance genes and genetic heterogeneity. CONCLUSIONS: Our study implicates aquatic food as beeing associated with mcr-1 carriage in the healthy population, even after the ban on colistin. Dietary modification (e.g. whole grains) may help to combat mcr-1-positive bacterial colonization of the gut.

12.
Echocardiography ; 38(8): 1474-1477, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34286882

RESUMO

A primary cardiac angiosarcoma (PCA) is very rare, highly aggressive, and metastatic in nature. The manifestations are often nonspecific, and the overall prognosis is extremely poor. The diagnosis of PCA can be complex and remains challenging. The key for diagnosis is echocardiography, and multimodality imaging is a more advantageous modality. Here, we present a rare case of a 41-year-old man who was diagnosed with PCA with multiple lung and bone metastases using multimodal imaging technology. Our case emphasizes the value of multimodality imaging in diagnosing PCA and suggests that patients showing intra-cardiac thrombus and hemorrhagic effusion on echocardiography without a clear explanation should be further investigated or closely followed.


Assuntos
Neoplasias Cardíacas , Hemangiossarcoma , Adulto , Ecocardiografia , Neoplasias Cardíacas/diagnóstico por imagem , Hemangiossarcoma/diagnóstico por imagem , Humanos , Masculino , Imagem Multimodal , Prognóstico
13.
Mol Ecol Resour ; 21(7): 2504-2519, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34133844

RESUMO

Areca palm (Areca catechu L.; family Arecaceae) is an important tropical medicinal crop and is also used for masticatory and religious purposes in Asia. Improvements to areca properties made by traditional breeding tools have been very slow, and further advances in its cultivation and practical use require genomic information, which is still unavailable. Here, we present a chromosome-scale reference genome assembly for areca by combining Illumina and PacBio data with Hi-C mapping technologies, covering the predicted A. catechu genome length (2.59 Gb, variety "Reyan#1") to an estimated 240× read depth. The assembly was 2.51 Gb in length with a scaffold N50 of 1.7Mb. The scaffolds were then further assembled into 16 pseudochromosomes, with an N50 of 172 Mb. Transposable elements comprised 80.37% of the areca genome, and 68.68% of them were long-terminal repeat retrotransposon elements. The areca palm genome was predicted to harbour 31,571 protein-coding genes and overall, 92.92% of genes were functionally annotated, including enriched and expanded families of genes responsible for biosynthesis of flavonoid, anthocyanin, monoterpenoid and their derivatives. Comparative analyses indicated that A. catechu probably diverged from its close relatives Elaeis guineensis and Cocos nucifera approximately 50.3 million years ago (Ma). Two whole genome duplication events in areca palm were found to be shared by palms and monocots, respectively. This genome assembly and associated resources represents an important addition to the palm genomics community and will be a valuable resource that will facilitate areca palm breeding and improve our understanding of areca palm biology and evolution.


Assuntos
Areca , Melhoramento Vegetal , Cromossomos , Genoma , Genômica , Filogenia
14.
Nanotechnology ; 32(37)2021 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-34087812

RESUMO

The stable hydrogen generation catalysts with favorable performance have been widely studied. As we all know, bimetallic hybrids have been emerged as one type of outstanding electrocatalysts and applied to electrochemical hydrogen generation field. In this work, using bimetallic polyoxometalates (POM) as soft templates and polyethyleneimine (PEI) as carbon source, we synthesized cobalt and tungsten-based bimetallic electrocatalysts (Co/WC@NC) via one-step simple high temperature calcination process.Co/WC@NCexhibits good hydrogen evolution performances. Thereinto, the overpotentials are 129 and 142 mV at the current density of 10 mA cm-2, and the Tafel slopes are 93 and 91 mV dec-1in 0.5 M H2SO4and 1.0 M KOH aqueous solutions, respectively. Furthermore, the catalyst shows good stability for 24 h. The excellent catalytic performance (Co/WC@NC) can be ascribed to the synergistic effect of WC and Co nanoparticles and the protection function of graphitic carbon layers. Furthermore, this work may provide an experimental guidance for the large-scale production of low-cost materials with good catalytic effects.

15.
J Air Waste Manag Assoc ; 71(11): 1361-1374, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34096835

RESUMO

Along with the increasingly stringent dust emission concentration standards for the thermal power plants and the challenge of market competition, wet electrostatic precipitators (ESPs) have been widely used as terminal equipment for the flue gas treatment. The wet ESPs pilot test platform using the modified carbon steel collectors with hydrophilic properties was established in this paper and the experimental research on PM2.5 removal characteristics were carried out. The mechanism of the particles removal efficiency enhancement by water film on the surface of modified collectors was studied. The effect of gas temperature, residence time, working voltage, inlet concentration, and flushing water flowrate on the particles removal efficiency was investigated. The results indicated that the fibrous layer over the surface of the modified collectors could reduce the effect of the airflow recoil and the electromigration resistance of particles. The surface of the modified collectors could also maintain a uniform and stable water film with a lower amount of water consumption. The water film could restrain the back corona and the re-entrainment of dust. And the evaporation of water film increased the humidity of the flue gas, meanwhile, the particles charge capacity and electromigration velocity increased. Those all could improve the particles removal efficiency. The particles removal efficiency increased with the increase of the flue gas residence time and the applied voltage, but the particles removal efficiency improved less with the increase of the particles inlet concentration and flushing water flow rate after maintaining uniform and stable water film. The modified rigid collector provided high removal efficiency for particles with diameters of 0.04~0.48 µm under lower energy and water consumption.Implications: The wet electrostatic precipitators (ESPs) have been widely used as terminal equipment of the flue gas treatment to purify the flue gas deeply. The surface of the modified carbon steel collectors with hydrophilic properties could maintain a uniform and stable water film with a lower amount of water consumption. The evaporation of water film improved the particle charge capacity and electromigration velocity. The wet ESPs using the modified rigid collector exhibited significantly higher removal efficiency of particles than using the conventional rigid collector especially to the particles with diameter of 0.04~0.48 µm.


Assuntos
Poluentes Atmosféricos , Poluentes Atmosféricos/análise , Carbono , Poeira , Tamanho da Partícula , Eletricidade Estática , Aço
17.
J Clin Neurosci ; 89: 243-248, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34119275

RESUMO

The sortilin-related receptor 1 (SORL1) gene has been the subject of many studies focusing on frequent polymorphisms, which is associated with increased risk for Alzheimer's Disease (AD). By whole-exome sequencing (WES), we identified two pathogenic missense mutations c.579C > G (p.F193L) and c.1397A > G (p.N466S) in SORL1. The two mutations were located in the same protein domain, and the two unrelated probands both had an onset of memory problems at less than 65 years of age, but their clinical manifestations and cranial imaging are different. The protein structure and function affected by these mutations were predicted using bioinformatics analysis, which suggested they were pathogenic. 3D protein structural analysis revealed that these amino acid substitutions might result in instability of protein structure and adverse intramolecular interactions. These findings suggest that both F193L and N466S should be thought as potential causative mutations in early-onset Alzheimer's disease (EOAD) patients. Further functional studies are warranted to evaluate their roles in the pathogenesis of AD.


Assuntos
Doença de Alzheimer/diagnóstico , Doença de Alzheimer/genética , Proteínas Relacionadas a Receptor de LDL/genética , Proteínas de Membrana Transportadoras/genética , Mutação/genética , Adulto , Sequência de Aminoácidos , Feminino , Predisposição Genética para Doença/genética , Humanos , Proteínas Relacionadas a Receptor de LDL/química , Masculino , Proteínas de Membrana Transportadoras/química , Pessoa de Meia-Idade , Linhagem , Estrutura Secundária de Proteína , Fatores de Risco , Sequenciamento Completo do Exoma/métodos
18.
BMC Infect Dis ; 21(1): 597, 2021 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-34157983

RESUMO

BACKGROUND: Klebsiella pneumoniae is a primary pathogen of pyogenic liver abscess (PLA). However, little data are available on combination with sepsis. In this study, we aimed to evaluate the clinical characteristics and prognostic differences of PLA patients with sepsis. METHODS: This retrospective cohort study was conducted to investigate 135 patients with confirmed Klebsiella pneumoniae-caused liver abscesses (KPLA) from a tertiary teaching hospital, from 2013 to 2019. The patients were divided into two groups, KPLA with sepsis and KPLA without sepsis. The demographic characteristics, clinical features as well as laboratory and microbiologic findings were analyzed. RESULTS: A total of 135 patients with KPLA were analyzed. The mean age of patients was 60.9 ± 12.7 years, and the percentage of men was 59.3%. Among them, 37/135 (27.4%) of patients had sepsis and the mortality rate was 1.5%. The most common symptom was fever (91.1%). KPLA patients with sepsis had a significantly higher proportion of frailty, diarrhea, fatty liver, chronic renal insufficiency, and hepatic dysfunction compared to KPLA patients without sepsis (p < 0.05). Antibiotic therapy and percutaneous drainage were most frequently therapeutic strategy. Furthermore, the incidences of sepsis shock and acute respiratory distress syndrome were higher in the sepsis group compared to the non-sepsis group. As for metastatic infections, the lung was the most common site. In addition, KPLA patients with sepsis showed respiratory symptoms in 11 patients, endophthalmitis in 4 patients, and meningitis in 1 patient. CONCLUSION: Our findings emphasize that KPLA patients combined with or without sepsis have different clinical features, but KPLA patients with sepsis have higher rates of complications and metastatic infections. Taken together, further surveillance and control of septic spread is essential for KPLA patients.


Assuntos
Infecções por Klebsiella/complicações , Klebsiella pneumoniae , Abscesso Hepático Piogênico/complicações , Sepse/etiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos
19.
Mol Biol Rep ; 48(3): 2365-2375, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33792826

RESUMO

Epithelial-mesenchymal transition (EMT), transforming growth factor ß(TGF-ß) and reactive oxygen species(ROS) plays a central role in cancer metastasis. Moreover, nicotinamide adenine dinucleotide phosphate 4(NOX4) is one of the main sources of ROS in lung cancer cells suggesting that NOX4 is associated with tumor cell migration. NF-κB(Nuclear factor-Kappa-B) is known to regulate ROS-mediated EMT process by activating Snail transcription factor in A549 cells. The purpose of this study was to explore the relationship between NF-κB and NOX4 in ROS production during TGF-ß induced EMT process. Several fractions have been pooled to evaluates the EMT process on lung cancer cells through real-time PCR, Western Blot and flow cytometry with DCFH-DA probe etc. Cells proliferation and migration activities were monitored by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay and wound healing assay respectively. The result showed that TGF-ß induction decreased the expression of E-cadherin, increased the Vimentin and the EMT transcription factor Snail in A549 cells. DPI (Diphenyleneiodonium chloride, an inhibitor of NOX4) inhibited the NOX4 expression and reduced ROS production induced by TGF-ß, but didn't affect the activation of NF-κB induced by TGF-ß (P > 0.05). BAY11-7082 (an inhibitor of NF-κB) inhibited the NF-κB (p65) expression and prevented the increase of NOX4 expression and ROS production induced by TGF-ß (P < 0.001), which has also verified reduced TGF-ß induced cell migration by inhibiting the EMT process, and also reduced cell proliferation of A549 cells (P < 0.001). The current research confirmed the TGF-ß mediated EMT process via NF-κB/NOX4/ROS signaling pathway, NF-κB and NOX4 are likely to be the potential therapeutic targets for lung cancer metastasis.


Assuntos
Transição Epitelial-Mesenquimal , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patologia , NADPH Oxidase 4/metabolismo , NF-kappa B/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais , Fator de Crescimento Transformador beta/metabolismo , Células A549 , Movimento Celular , Proliferação de Células , Humanos
20.
Int J Parasitol Parasites Wildl ; 14: 211-215, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33898222

RESUMO

Enterocytozoon bieneusi is a zoonotic pathogen that infects a variety of hosts including humans, livestock, wildlife, companion animals, and birds, as well as being abundant in the environment. Humans and nonhuman animals could be infected with E. bieneusi via consumption of food or water that contains zoonotic and host-adapted genotypes. In this study, 288 fecal specimens were collected from farmed minks, blue foxes, and raccoon dogs, in Xinjiang, China. Enterocytozoon bieneusi was examined by PCR amplification based on sequence analysis of the internal transcribed spacer (ITS) region. The overall infection rate of E. bieneusi was 4.9% (14/288), with mink samples showing the highest infection rate (5.6%, 12/214), followed by blue foxes (2.9%, 1/35), and then raccoon dogs (2.6%, 1/39). Six E. bieneusi genotypes were identified, including D (n = 5), PigEBITS7 (n = 4), EbpA (n = 2), CAM5 (n = 1), WildBoar3 (n = 1), and a novel genotype XJMI-1 (n = 1). Phylogenetic analysis showed that all E. bieneusi genotypes belonged to group 1, which composed of over 300 genotypes and most of them have been identified in human and variety of animals, suggesting a risk of zoonotic transmission from farmed wildlife to humans.

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