Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 225
Filtrar
1.
Eur J Clin Nutr ; 2022 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-36100703

RESUMO

BACKGROUND/OBJECTIVES: The evidence of relationship between dietary intake of folate, vitamin B6 and vitamin B12 and cardiovascular diseases (CVD) in UK populations is limited. We aimed to analyze the association of dietary intake of folate, vitamin B6, and vitamin B12 with CVD events [stroke, myocardial infarction (MI)] and CVD mortality. METHODS: We included 115,664 participants, aged 40-70 years, with no CVD events or cancer at baseline, enrolled between 2006 and 2010 and followed up to the end of 2018. Dietary intake was measured with an online 24-h dietary assessment. Multivariable Cox proportional hazards models were used to estimate hazard ratios (HR) and 95% confidence intervals (CI) for the associations. RESULTS: After multivariate adjustment, higher dietary folate intake was inversely associated with CVDs with hazard ratios of 0.99, 0.92, and 0.88 in groups 2-4 compared with group 1 (the lowest group); inversely associated with stroke with hazard ratios of 0.94, 0.90, and 0.86 groups 2-4 compared to group 1 (lowest group); inversely associated with MI with hazard ratios of 1.01, 0.90 and 0.86 groups 2-4 compared to group 1 (lowest group); inversely associated with CVD mortality with hazard ratios of 0.95, 0.80 and 0.74 Groups 2-4 compared to group 1 (lowest group). Each tablespoon/day higher intake of raw vegetable intake, pieces/day higher intake of fresh fruit intake bowls/week higher intake of cereal intake, and g/day higher intake of dietary fiber were associated with higher intakes of folate every 0.02,0.06,0.05, and 0.08 SD, respectively. E-value analysis suggested robustness to unmeasured confounding. CONCLUSIONS: Each increase in folate intakes was related to 5% lower risks of total CVD events and 10% lower risks of CVD mortality. Our findings support that strengthening dietary folate intake as a primary prevention strategy for CVD events and CVD mortality.

2.
Oncol Rep ; 48(5)2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36129149

RESUMO

AR signalling pathway reactivation plays a key role in the development of castration­resistant prostate cancer (CRPC). High­mobility group protein B1 (HMGB1) is an important factor involved in the occurrence and development of a variety of tumours by regulating gene transcription. In the present study, the association between HMGB1 and prostate cancer (PCa) and the effects of HMGB1 on androgen receptor (AR) transcription and signalling pathway reactivation in PCa cells in vitro and in vivo were evaluated. A bioinformatics method was used to determine the mRNA expression level of HMGB1 in PCa specimens and its correlation with the mRNA expression of AR. Immunohistochemical staining was used to detect the expression of these proteins in clinical PCa samples. Reporter gene and ChIP assays were performed to determine the activity of AR and the effect of HMGB1 on the ability of AR to bind to the promoters of prostate specific antigen and transmembrane protease, serine 2. A bioluminescence resonance energy transfer assay was employed to observe the direct interaction between HMGB1 and AR protein. Additionally, a castrated nude mouse xenograft tumour model was established to verify the effect of HMGB1 on PCa. The results revealed that HMGB1 expression was significantly increased in PCa specimens, which may have a strong correlation with AR expression. Moreover, HMGB1 could reactivate the AR signalling pathway, directly interact with AR, and promote the development of CRPC in an androgen­independent manner. The results of the present study indicated that HMGB1 promoted the development of CRPC by interacting with AR, which inferred that decreasing the expression of HMGB1 may be a potential effective method for CRPC prevention and treatment.


Assuntos
Proteína HMGB1 , Neoplasias de Próstata Resistentes à Castração , Androgênios , Animais , Proteína HMGB1/genética , Humanos , Masculino , Camundongos , Camundongos Nus , Antígeno Prostático Específico , Neoplasias de Próstata Resistentes à Castração/tratamento farmacológico , RNA Mensageiro/genética , Receptores Androgênicos/genética , Receptores Androgênicos/metabolismo
3.
Langmuir ; 38(37): 11137-11148, 2022 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-36070512

RESUMO

Understanding the behaviors of nanoparticles at interfaces is crucial not only for the design of novel nanostructured materials with superior properties but also for a better understanding of many biological systems where nanoscale objects such as drug molecules, viruses, and proteins can interact with various interfaces. Theoretical studies and tailored computer simulations offer unique approaches to investigating the evolution and formation of structures as well as to determining structure-property relationships regarding the interfacial nanostructures. In this feature article, we summarize our efforts to exploit computational approaches as well as theoretical modeling in understanding the organization of nanoscale objects at the interfaces of various systems. First, we present the latest research advances and state-of-the-art computational techniques for the simulation of nanoparticles at interfaces. Then we introduce the applications of multiscale modeling and simulation methods as well as theoretical analysis to explore the basic science and the fundamental principles in the interfacial nanoparticle organization, covering the interfaces of polymer, nanoscience, biomacromolecules, and biomembranes. Finally, we discuss future directions to signify the framework in tailoring the interfacial organization of nanoparticles based on the computational design. This feature article could promote further efforts toward fundamental research and the wide applications of theoretical approaches in designing interfacial assemblies for new types of functional nanomaterials and beyond.


Assuntos
Nanopartículas , Nanoestruturas , Simulação por Computador , Modelos Teóricos , Nanopartículas/química , Nanoestruturas/química , Polímeros/química
4.
Environ Res ; 215(Pt 1): 114326, 2022 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-36113575

RESUMO

Diclofenac (DFC) is a pharmacologically active compound frequently detected in various receiving waters. To improve the efficiency of constructed wetlands in removing DFC, biochar (BC) is added as a substrate. The study mainly involved the effect of adding wetland plant-derived BC to vertical subsurface flow constructed wetlands (VSF-CWs) on the DFC removal process. In addition, the study discussed the effects of the initial DFC concentration (0.05-1.00 mg L-1), pH (5.5-8.5), and hydraulic retention times (HRTs, 1-7 d) on the removal process and fluctuations in the microbial community. Preliminary results of the study showed optimal removal (>90%) achieved at an initial DFC concentration of 0.75-1 mg L-1, a pH of 6.5-7.5, and an HRT of 7 d. Moreover, no significant effects on the removal efficiency of conventional water quality parameters were observed. Non-metric multidimensional scaling results revealed a reshaped community structure, which was altered by the initial DFC concentration. DFC concentration is a key factor in the variation of microbial communities and controls the quantitative evolution of the species in experimental units. Therefore, the addition of BC to CWs effectively enhanced the removal efficiency of DFC and provided a viable and effective improvement of the CWs.

5.
Front Immunol ; 13: 942897, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36091016

RESUMO

Ebola virus (EBOV), a member of the Filoviridae family of viruses and a causative agent of Ebola Virus Disease (EVD), is a highly pathogenic virus that has caused over twenty outbreaks in Central and West Africa since its formal discovery in 1976. The only FDA-licensed vaccine against Ebola virus, rVSV-ZEBOV-GP (Ervebo®), is efficacious against infection following just one dose. However, since this vaccine contains a replicating virus, it requires ultra-low temperature storage which imparts considerable logistical challenges for distribution and access. Additional vaccine candidates could provide expanded protection to mitigate current and future outbreaks. Here, we designed and characterized two multimeric protein nanoparticle subunit vaccines displaying 8 or 20 copies of GPΔmucin, a truncated form of the EBOV surface protein GP. Single-dose immunization of mice with GPΔmucin nanoparticles revealed that neutralizing antibody levels were roughly equivalent to those observed in mice immunized with non-multimerized GPΔmucin trimers. These results suggest that some protein subunit antigens do not elicit enhanced antibody responses when displayed on multivalent scaffolds and can inform next-generation design of stable Ebola virus vaccine candidates.


Assuntos
Vacinas contra Ebola , Ebolavirus , Doença pelo Vírus Ebola , Nanopartículas , Animais , Anticorpos Neutralizantes , Anticorpos Antivirais , Camundongos
6.
J Control Release ; 349: 327-337, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35787917

RESUMO

Transcatheter arterial chemoembolization (TACE), the mainstream treatment for hepatocellular carcinoma (HCC), is a method of blocking tumor blood vessels with a mixture of lipiodol and chemotherapeutics. And the contrast-enhanced computed tomography (CT) is the commonly used way for follow-up of HCC after TACE. However, it is noteworthy that when lipiodol deposition plays an embolic effect, it also produces high-density artifacts in CT images. These artifacts usually conceal the enhancement effect of iodine contrast agents. As a result, the residual region is difficult to be visualized. To overcome this obstacle, we developed one kind of Lu3+/Gd3+ doped fluoride nanoprobe modified with Dp-PEG2000 to realize CT/MRI dual-modality imaging of HCC. Compared with lipiodol or ioversol, the obtained PEGylated product LG-PEG demonstrated a greater density value in high keV CT images. In vitro experiments showed the lipiodol artifacts can be removed in virtual non-contrast (VNC) imaging, but the density of ioversol was also removed at the same time. However, the LG-PEG synthesized in this work can still maintain a high density in VNC imaging, which indicates that LG-PEG can exploit its advantages to the full in VNC imaging. Furthermore, LG-PEG successfully exerted tumor enhancement effects in the in vivo VNC images of HCC with lipiodol deposition. In addition, LG-PEG exhibited a strong T2 enhancement effect with low biological toxicity and less side-effect on the main organ and blood. Thus, the LG-PEG reported in this research can serve as an effective and safe VNC contrast agent for HCC imaging after TACE.


Assuntos
Carcinoma Hepatocelular , Quimioembolização Terapêutica , Iodo , Neoplasias Hepáticas , Carcinoma Hepatocelular/diagnóstico por imagem , Carcinoma Hepatocelular/patologia , Carcinoma Hepatocelular/terapia , Quimioembolização Terapêutica/métodos , Meios de Contraste , Óleo Etiodado , Fluoretos , Humanos , Neoplasias Hepáticas/diagnóstico por imagem , Neoplasias Hepáticas/patologia , Neoplasias Hepáticas/terapia , Polietilenoglicóis , Tomografia Computadorizada por Raios X/métodos , Ácidos Tri-Iodobenzoicos
7.
Front Microbiol ; 13: 924172, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35783397

RESUMO

Human adenovirus type 21 (HAdV-21) is an important pathogen associated with acute respiratory infection (ARI), but it was rarely reported and characterized so far. In this study, 151 of 1,704 (8.9%) pediatric patients (≤14 years old) hospitalized with ARI in Guangzhou, China in 2019 were positive for HAdV which was the third most frequently detected pathogen. Two HAdV-21-positive patients presented with severe lower respiratory illness and had similar initial symptoms at onset of illness. Then two HAdV-21 strains were isolated and characterized. The two HAdV-21 strains were sequenced and classified as subtype 21a with genomes closely related to strain BB/201903 found in Bengbu, China in March 2019. Phylogenetic analysis for whole genome and major antigen proteins of global HAdV-21 strains showed that HAdV-21 could be classified into two branches, branch 1 including genotype 21p, branch 2 including all other strains dividing into genotype 21a and 21b. There was no significant difference in the plaque size, or the replication curves between the two HAdV-21a strains and the prototype strain HAdV-21p AV-1645. However, there were five highly variable regions (HVR1, HVR3, HVR4, HVR5, and HVR7) in the hexon protein that varied between two branches. Mice immunized with one branch strain showed 2-4-fold lower neutralizing antibody titers against another branch strain. In summary, this study firstly reported two HAdV-21a infections of children in China, characterized two isolates of HAdV-21a associated with severe lower respiratory illness; our results could be important for understanding the HAdV-21 epidemiology and pathogenic, and for developing HAdV-21 vaccine and drug.

8.
Front Cell Dev Biol ; 10: 921320, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35874811

RESUMO

Necroptosis, as a form of programmed cell death, is involved in many physiological and pathological processes. However, its role in cancer progression and therapeutic response remains controversial. Colon cancer is one of the leading causes of cancer death and patients' response to immune checkpoint blockade vary to a large degree. In this study, we investigated necroptosis related genes (NRGs) alterations in colon cancer by bioinformatics analysis. Colon cancer patients were classified into two subtypes with distinct clinical and molecular features based on NRGs. After finding differentially expressed genes and lasso regression, a prognostic model based on four necroptosis signature genes was constructed. The necroptosis signature was also a good predictor in the field of chemotherapy and immunotherapy in colon cancer. Altogether, this study illustrates the relationship between necroptosis and colon cancer, and establishes a novel scoring method to predict prognosis and therapeutic response in colon cancer patients.

9.
Neuron ; 110(15): 2409-2421.e3, 2022 08 03.
Artigo em Inglês | MEDLINE | ID: mdl-35679860

RESUMO

The action potential is a fundamental unit of neural computation. Even though significant advances have been made in recording large numbers of individual neurons in animal models, translation of these methodologies to humans has been limited because of clinical constraints and electrode reliability. Here, we present a reliable method for intraoperative recording of dozens of neurons in humans using the Neuropixels probe, yielding up to ∼100 simultaneously recorded single units. Most single units were active within 1 min of reaching target depth. The motion of the electrode array had a strong inverse correlation with yield, identifying a major challenge and opportunity to further increase the probe utility. Cell pairs active close in time were spatially closer in most recordings, demonstrating the power to resolve complex cortical dynamics. Altogether, this approach provides access to population single-unit activity across the depth of human neocortex at scales previously only accessible in animal models.


Assuntos
Neocórtex , Neurônios , Potenciais de Ação/fisiologia , Eletrodos , Eletrodos Implantados , Humanos , Neurônios/fisiologia , Reprodutibilidade dos Testes
10.
Materials (Basel) ; 15(11)2022 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-35683185

RESUMO

Aluminum alloy is widely used in aerospace structures. However, it often suffers from a harsh corrosion environment, resulting in different damage such as pitting corrosion, which leads to a reduction in the service life of aerospace structures. In the present study, the pitting corrosion with a radius of 1 mm and a depth of 0.6 mm was manufactured using hydrofluoric (HF) acid on a 2024-T3 aluminum alloy plate (400 mm × 400 mm × 2 mm) to simulate the corrosion state of equipment. A signal acquisition system with a square sensor network of 12 piezoelectric transducers (PZTs) was established. The sensor path weighting reconstruction algorithm for the probabilistic inspection of defects (SPW-RAPID) is proposed based on corrosion damage characteristic parameters including signal correlation coefficient (SDC), root mean squared error (RMSE), and signal energy damage index (E1) to explore the monitoring efficacy of pitting corrosion. The sensor path weight w, which is the product of value coefficient a and impact factor l, is established to modify the corrosion damage characteristic parameters. The results indicate that the SPW-RAPID algorithm can improve the accuracy and clarity of image reconstruction results based on SDC, RMSE and E1, which can locate the pitting corrosion with a radius of 1 mm and a depth of 0.6 mm, and the positioning error is controlled within 0.1 mm. The research work may provide an available way to monitor tiny corrosion damage on an aluminum alloy structure.

11.
Nutrients ; 14(11)2022 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-35684060

RESUMO

BACKGROUND AND AIMS: Crohn's disease (CD) is usually accompanied by malnutrition. CD-related malnutrition can increase morbidity, disability, mortality, and hospitalization costs. The purpose of this study was to find a reliable indicator for evaluating CD patients' nutritional status. METHODS: All data were retrospectively collected from Xiangya Hospital, Central South University between May 2021 and February 2022. All patients were evaluated for nutritional status using the Global Leadership Initiative on Malnutrition (GLIM) criteria. Body composition, resistance, and reactance were recorded by a body analyser, and the phase angle (PhA) was calculated simultaneously. The Mann-Whitney U test, chi-square test, Fisher's exact test, and univariate and multivariate logistic regression analyses were used. A receiver operating characteristic (ROC) curve was built to evaluate the predictive value of differential variables for diagnosing malnutrition based on the GLIM criteria. RESULTS: A total of 169 CD patients were enrolled, of which 74 (58.3%) males and 32 (76.2%) females were diagnosed with malnutrition; 34 (45.9%) males and 22 (68.8%) females were severely malnourished. Univariate analysis identified that as nutritional status deteriorated, body mass index, PhA, and levels of haemoglobin and albumin decreased, while platelet count, erythrocyte sedimentation rate, and levels of C-reactive protein and fibrinogen increased (p < 0.05). Logistic regression analysis revealed that the PhA was significantly independently associated with malnutrition (p < 0.05). The ROC curve analysis indicated that the optimal PhA cut-off levels of 6.11° and 5.55° could be used to predict malnutrition according to the GLIM criteria in males and females, respectively, with a PhA < 5.53° and < 5.12° indicating severe malnutrition in males and females, respectively. CONCLUSION: The PhA is a sensitive, noninvasive, portable, inexpensive tool that can be used to monitor and manage the nutritional status of CD patients.


Assuntos
Doença de Crohn , Desnutrição , Doença de Crohn/complicações , Doença de Crohn/diagnóstico , Impedância Elétrica , Feminino , Humanos , Masculino , Desnutrição/diagnóstico , Desnutrição/etiologia , Avaliação Nutricional , Estado Nutricional , Estudos Retrospectivos
12.
Front Neurol ; 13: 871421, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35645955

RESUMO

Objective: Cerebral ischemia and intracranial hemorrhage are the two main phenotypes of moyamoya disease (MMD). However, the pathophysiological processes of these two MMD phenotypes are still largely unknown. Here, we aimed to use multimodal neuroimaging techniques to explore the brain structural and functional differences between the two MMD subtypes. Methods: We included 12 patients with ischemic MMD, 10 patients with hemorrhagic MMD, and 10 healthy controls (HCs). Each patient underwent MRI scans and cognitive assessment. The cortical thickness of two MMD subtypes and HC group were compared. Arterial spin labeling (ASL) and diffusion tensor imaging (DTI) were used to inspect the cerebral blood flow (CBF) of cortical regions and the integrity of related white matter fibers, respectively. Correlation analyses were then performed among the MRI metrics and cognitive function scores. Results: We found that only the cortical thickness in the right middle temporal gyrus (MTG) of hemorrhagic MMD was significantly greater than both ischemic MMD and HC (p < 0.05). In addition, the right MTG showed higher ASL-CBF, and its associated fiber tract (arcuate fasciculus, AF) exhibited higher fractional anisotropy (FA) values in hemorrhagic MMD. Furthermore, the cortical thickness of the right MTG was positively correlated with its ASL-CBF values (r = 0.37, p = 0.046) and the FA values of right AF (r = 0.67, p < 0.001). At last, the FA values of right AF were found to be significantly correlated with cognitive performances within patients with MMD. Conclusions: Hemorrhagic MMD shows increased cortical thickness on the right MTG in comparison with ischemic MMD and HCs. The increased cortical thickness is associated with the higher CBF values and the increased integrity of the right AF. These findings are important to understand the clinical symptoms and pathophysiology of MMD and further applied to clinical practice.

13.
BMC Neurol ; 22(1): 179, 2022 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-35578209

RESUMO

BACKGROUND: Disruption of brain functional connectivity has been detected after stroke, but whether it also occurs in moyamoya disease (MMD) is unknown. Impaired functional connectivity is always correlated with abnormal white matter fibers. Herein, we used multimodal imaging techniques to explore the changes in brain functional and structural connectivity in MMD patients. METHODS: We collected structural images, resting-state functional magnetic resonance imaging (rs-fMRI) and diffusion tensor imaging for each subject. Cognitive functions of MMD patients were evaluated using the Mini-Mental State Examination (MMSE), Montreal Cognitive Assessment (MoCA), and Trail Making Test parts A and B (TMT-A/-B). We calculated the functional connectivity for every paired region using 90 regions of interest from the Anatomical Automatic Labeling Atlas and then determined the differences between MMD patients and HCs. We extracted the functional connectivity of paired brain regions with significant differences between the two groups. Correlation analyses were then performed between the functional connectivity and variable cognitive functions. To explore whether the impaired functional connectivity and cognitive performances were attributed to the destruction of white matter fibers, we further analyzed fiber integrity using tractography between paired regions that were correlated with cognition. RESULTS: There was lower functional connectivity in MMD patients as compared to HCs between the bilateral inferior frontal gyrus, between the bilateral supramarginal gyrus, between the left supplementary motor area (SMA) and the left orbital part of the inferior frontal gyrus (IFGorb), and between the left SMA and the left middle temporal gyrus (P < 0.01, FDR corrected). The decreased functional connectivity between the left SMA and the left IFGorb was significantly correlated with the MMSE (r = 0.52, P = 0.024), MoCA (r = 0.60, P = 0.006), and TMT-B (r = -0.54, P = 0.048) in MMD patients. White matter fibers were also injured between the SMA and IFGorb in the left hemisphere and were positively correlated with reduced functional connectivity. CONCLUSIONS: Brain functional and structural connectivity between the supplementary motor area and inferior frontal gyrus in the left hemisphere are damaged in MMD. These findings could be useful in the evaluation of disease progression and prognosis of MMD.


Assuntos
Córtex Motor , Doença de Moyamoya , Encéfalo/diagnóstico por imagem , Mapeamento Encefálico , Imagem de Tensor de Difusão , Humanos , Imageamento por Ressonância Magnética , Córtex Motor/diagnóstico por imagem , Doença de Moyamoya/diagnóstico por imagem , Córtex Pré-Frontal
14.
Mol Imaging Biol ; 2022 May 25.
Artigo em Inglês | MEDLINE | ID: mdl-35612771

RESUMO

PURPOSE: Early diagnosis of sepsis-associated encephalopathy (SAE) is essential for the treatment and prognosis of septic patients. Static PET and MRI have shown promise for early diagnosis, while pharmacokinetic parameters from dynamic PET may provide better quantification for SAE. This study aims to compare the performance of dynamic 2-deoxy-2-[18F]fluoro-D-glucose ([18F]F-FDG) PET and multiparametric MRI in early imaging SAE with a view to providing guidance for the early diagnosis of SAE. PROCEDURES: Dynamic [18F]F-FDG-PET/CT scans and multiparametric MRI were performed in SAE mice induced by LPS. Standardized uptake value (SUV) was measured in static scan images and [18F]F-FDG pharmacokinetic parameters were analyzed with two-tissue compartment model and Patlak plot. MRI relative signal intensity (rT1) derived from T1-weighted images (pre and post contrast) and 4 parameters originating from diffusion-weighted data were measured. RESULTS: Both SUV and dephosphorylation rate constant (k4) increased in SAE model as early as 6 h post sepsis induction, while k4 increased with the relative value (SAE/normal) significantly stronger than that of SUV. Moreover, the net influx constant (Ki) showed significant decrease in SAE as early as 6 h compared with normal mice. Increased signal intensity was identified in T1-weighted contrast enhanced images and rT1 value increased at 12 h post induction. Diffusion tensor imaging (DTI) revealed fractional anisotropy (FA) decreased at 12 h and 24 h in external capsule (ec) and declined axial diffusivity (AD) was shown in white matter at 24 h. CONCLUSIONS: The dynamic PET (k4) was more sensitive than static PET (SUV) for early diagnosis of SAE and declined Ki was firstly found in murine SAE, which indicated the advantage of dynamic PET/CT in early detection and differential diagnosis of SAE. While MRI has a higher soft tissue resolution than PET/CT and can classify more subtle brain areas, the comprehensive utilization of the two modalities is helpful for managing SAE.

15.
Science ; 376(6596): eabe1505, 2022 05 27.
Artigo em Inglês | MEDLINE | ID: mdl-35617398

RESUMO

In castration-resistant prostate cancer (CRPC), the loss of androgen receptor (AR) dependence leads to clinically aggressive tumors with few therapeutic options. We used ATAC-seq (assay for transposase-accessible chromatin sequencing), RNA-seq, and DNA sequencing to investigate 22 organoids, six patient-derived xenografts, and 12 cell lines. We identified the well-characterized AR-dependent and neuroendocrine subtypes, as well as two AR-negative/low groups: a Wnt-dependent subtype, and a stem cell-like (SCL) subtype driven by activator protein-1 (AP-1) transcription factors. We used transcriptomic signatures to classify 366 patients, which showed that SCL is the second most common subtype of CRPC after AR-dependent. Our data suggest that AP-1 interacts with the YAP/TAZ and TEAD proteins to maintain subtype-specific chromatin accessibility and transcriptomic landscapes in this group. Together, this molecular classification reveals drug targets and can potentially guide therapeutic decisions.


Assuntos
Cromatina , Terapia de Alvo Molecular , Neoplasias de Próstata Resistentes à Castração , Linhagem Celular Tumoral , Cromatina/genética , Perfilação da Expressão Gênica , Humanos , Masculino , Células-Tronco Neoplásicas/classificação , Células-Tronco Neoplásicas/metabolismo , Organoides/metabolismo , Organoides/patologia , Neoplasias de Próstata Resistentes à Castração/classificação , Neoplasias de Próstata Resistentes à Castração/tratamento farmacológico , Neoplasias de Próstata Resistentes à Castração/genética , Receptores Androgênicos/genética , Receptores Androgênicos/metabolismo , Fator de Transcrição AP-1/genética , Fator de Transcrição AP-1/metabolismo
16.
Chemosphere ; 303(Pt 1): 134990, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35595118

RESUMO

Massive prevalence of microplastics (MPs) in the environment has become one of the world's most serious environmental concerns. Human dependence on plastics has created a constant flow of MPs from different sources into natural environment, which has raised public concern regarding consequences of MPs coming into contact with the natural environment. Deploying constructed wetlands (CWs) to reduce MPs pollution is considered a promising method, however there are still barriers for breakthroughs in this technology, particularly knowledge gaps in the mechanisms affect removal process. Recognising this, we provide a comprehensive summary of current advances and theories regarding the mechanisms of occurrence in this research area. In this work, the bibliometric methods were first used to identify annual publication trends and topical topics of research interest. The selected documents were then statistically analyzed using VOSviewer and the 'bibliometrix' package in R to derive the annual productivity of countries or organizations, the most relevant affiliations, the most relevant authors, the most relevant sources, textual analysis, co-occurrence analysis, and cluster analysis of keywords. Finally, detailed information concerning the removal of MPs by CWs was summarised, covering the most common operational and design parameters (i.e., structure types, wetland plants, substrate materials, and microbial communities), to reveal how these parameters can be adjusted for more efficient MPs removal rate. Challenges and future directions were additionally proposed. It is hoped that the review will help identify current research trends, provide insight into the mechanisms of the removal process, and contribute further to the development of this important area.


Assuntos
Microplásticos , Poluentes Químicos da Água , Bibliometria , Poluição Ambiental , Humanos , Plásticos , Poluentes Químicos da Água/análise , Áreas Alagadas
17.
J Hazard Mater ; 435: 129028, 2022 08 05.
Artigo em Inglês | MEDLINE | ID: mdl-35525009

RESUMO

Heavy metal pollution in water seriously affects human health. The disadvantages of traditional metal ion detection methods involve long and cumbersome chemical pretreatment in the early stage, and large volume of samples. In this study, microalgae were used as the medium, and terahertz spectroscopy technology was employed to collect the changes of material components in it, so as to deduce the types and concentrations of heavy metal pollution in water. Through the partial least square(PLS), we establish the prediction model of heavy metal concentration, and the results show that the best detection time for Pb2+ is 6 h and Ni2+ is 18 h. The principal component analysis(PCA) shows that ß-carotene is the most affected substance. Afterward we collect five real surface waters in East China and verify that the judgment accuracy of Pb2+ and Ni2+ are 100% and 93.2% respectively. The results indicate that the time is shorter than the traditional pretreatment time from more than 20-6 h, the sample volume is reduced from 50 mL to 10 mL, the detection accuracy is improved from 10 ng/mL to 1 ng/mL. In a word, we provide a new fast and real-time method for biological monitoring of heavy metal pollution in water.


Assuntos
Metais Pesados , Microalgas , Poluentes Químicos da Água , China , Monitoramento Ambiental/métodos , Humanos , Íons , Chumbo , Metais Pesados/análise , Tecnologia , Água , Poluentes Químicos da Água/análise
18.
Environ Sci Pollut Res Int ; 29(42): 63735-63752, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35461412

RESUMO

A reliable and simple analytical method was developed and validated to simultaneously determine chlorantraniliprole and indoxacarb in tea using ultrahigh-performance liquid chromatography-tandem mass spectrometry. The average recoveries of chlorantraniliprole were in the range of 86-110%, with the precision of intraday (n = 5) and interday (n = 15) ranging from 1.9 to 8.4% and from 2.4 to 8.8%, respectively. The average recoveries of indoxacarb were in the range 81-105%, with the precision of intraday (n = 5) and interday (n = 15) ranging from 2.0 to 9.8% and from 2.7 to 9.1%, respectively. The limits of quantification (LOQs) were all 0.01 mg/kg. The results based on the supervised field trials showed that chlorantraniliprole and indoxacarb in two tea samples followed first-order kinetics models with half-lives of 2.2-4.7 days and 2.5-3.5 days, which could be regarded as a moderately degrading pesticide. The terminal residues of chlorantraniliprole and indoxacarb in made tea were below 6.7 and 4.5 mg/kg, respectively, lower than their corresponding maximum residue limits (MRLs) established by several major countries and organizations (50 and 5 mg/kg). The leaching rates of chlorantraniliprole and indoxacarb during the tea brewing ranged from 4.78 to 12.62% and 4.13 to 10.67%, respectively. The chronic intake risk quotient (RQc) values for chlorantraniliprole and indoxacarb were 0.24% and 35.10%, while the acute dietary risk assessment (RQa) value of indoxacarb was 5.8%, which were all much lower than 100%. The results in the present study indicated that the health risk posed by the chlorantraniliprole and indoxacarb mixture pesticides was negligible in tea for consumers at the recommended dosages.


Assuntos
Resíduos de Praguicidas , Praguicidas , Cromatografia Líquida de Alta Pressão , Oxazinas , Resíduos de Praguicidas/análise , Praguicidas/análise , Espectrometria de Massas em Tandem/métodos , Chá/química , ortoaminobenzoatos
20.
J Sci Food Agric ; 102(13): 5826-5836, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35426151

RESUMO

BACKGROUND: Recently, chlorfenapyr and diafenthiuron have been widely used to prevent and control diseases and pests in tea production. However, rare studies have investigated the dissipation patterns of chlorfenapyr, diafenthiuron and their metabolites simultaneously in tea matrices. Here, we established an analytical method to investigate the degradation patterns of five target compounds in tea shoots and made tea samples. Moreover, the dietary intake risk assessment of chlorfenapyr-diafenthiuron mixture among Chinese populations was evaluated based on the supervised field experiment. RESULTS: The mean recoveries of the primary analytes at five spiking levels were between 95.6% and 112.6% in tea shoots and made tea, respectively, and the values of RSD (relative standard deviation) were lower than 9.7% for all the target analytes. The field trial results showed that the half-lives of chlorfenapyr and diafenthiuron based on the residue definition were 10.0-12.4 days and 4.3-5.9 days, respectively, in tea shoots. For the dietary intake risk assessment, the risk quotient (RQ) values in made tea ranged from 30.4% to 73.9% at the pre-harvest interval of 14 days, which were significantly less than 100%. CONCLUSION: The accuracy and precision of the developed method were satisfied by the measurement requirements according to the validation results. The dynamic dissipation experiments suggested that diafenthiuron was much easier to dissipate than chlorfenapyr. Moreover, the existence of tralopyril made the half-life of chlorfenapyr significantly increase, indicating that practical application of chlorfenapyr should take careful consideration of its metabolite. Finally, the potential chronic dietary risks of the chlorfenapyr-diafenthiuron mixture to human communities were within the acceptable range. © 2022 Society of Chemical Industry.


Assuntos
Resíduos de Praguicidas , Ingestão de Alimentos , Humanos , Resíduos de Praguicidas/análise , Feniltioureia/análogos & derivados , Piretrinas , Medição de Risco , Chá/química , Árvores
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...