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1.
J Environ Sci (China) ; 124: 198-204, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36182131

RESUMO

Air pollutants can potentially lead to nitration of allergic proteins, thus promoting sensitization of these allergens. However, little is currently known about the nitration status of house dust mite (HDM) allergens. We identified the occurrence of nitrated products of two major HDM allergens Der f 1 and Der p 1 in dust samples collected from college dormitories in eastern China and assessed their associated health risk. The results showed that both non-nitrated and nitrated forms of the two allergens were detected in the dust in the range of non-detected (ND)-10.6, 1.44-15.4, ND-22.4, ND-7.28 µg/g for non-nitrated Der f 1, nitrated Der f 1, non-nitrated Der p 1 and nitrated Der p 1, respectively. The median rates of nitration were determined as 74.0% for Der f 1 and 20.4% for Der p 1 at consideration of one nitration site. Further analysis reveals that the levels of HDM allergens and their nitrated products were found to be generally higher during winter, in dormitories of lower altitude and with female occupants. Furthermore, the calculated risk indexes were at considerably high levels. Our findings suggest that nitrated HDM allergens have already accumulated in the environment at such significant levels and their associated health risk calls for our immediate attention.


Assuntos
Poluentes Atmosféricos , Poluição do Ar em Ambientes Fechados , Poluentes Atmosféricos/análise , Poluição do Ar em Ambientes Fechados/análise , Alérgenos/análise , Animais , Antígenos de Dermatophagoides/análise , Poeira/análise , Feminino , Humanos , Nitratos/análise , Óxidos de Nitrogênio/análise , Compostos Orgânicos/análise , Pyroglyphidae , Medição de Risco
3.
J Stroke Cerebrovasc Dis ; 31(12): 106857, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36334373

RESUMO

OBJECTIVE: To compare the difference in the risk of stroke for four kinds of carotid artery plaque and carotid artery stenosis. METHODS: Literature was collected by searching the PubMed, Embase, Cochrane library and Ovid databases up to June 2022, using the free search terms "carotid plaque" and "stroke". Meta-analysis was performed on the selected articles using Stata16 to analyse the relationship of stroke risk factors. RESULTS: A total of 11 studies including 6661 participants were included. Meta-analysis results showed that the incidence of stroke was statistically significantly different between IPH (intraplaque haemorrhage) plaques and LRNC (lipid-rich necrotic core) plaques (RR: 1.27, 95% CI: 1.04-1.55, P < 0.05) and IPH plaques and calcification plaques (RR: 2.99, 95% CI: 1.74-5.14, P < 0.0001). Furthermore, there was a statistically significant difference between TRFC (thinned or ruptured fibrous caps) plaques and carotid artery stenosis (RR: 10.84, 95% CI: 5.60-20.98, P < 0.0001) and calcification plaques and carotid artery stenosis (RR: 0.83, 95% CI: 0.75-0.92, P < 0.0001). However, there was no statistically significant difference between the IPH and carotid artery stenosis (RR: 1.55, 95% CI: 0.68-3.52, P > 0.05), LRNC and TRFC (RR: 0.80, 95% CI: 0.11-5.82, P > 0.05), LRNC and calcification (RR: 1.81, 95% CI: 0.90-3.66, P > 0.05) and LRNC and carotid artery stenosis (RR: 1.40, 95% CI: 0.69-2.81, P > 0.05). CONCLUSION: IPH was associated with a higher incidence of stroke compared to LRNC and calcification plaques and TRFC has a higher risk of stroke than calcification plaques and carotid stenosis. This evidence suggests that IPH and TRFC may play an important role in predicting stroke.


Assuntos
Estenose das Carótidas , Placa Aterosclerótica , Acidente Vascular Cerebral , Humanos , Estenose das Carótidas/complicações , Estenose das Carótidas/diagnóstico por imagem , Estenose das Carótidas/epidemiologia , Artérias Carótidas/diagnóstico por imagem , Placa Aterosclerótica/complicações , Acidente Vascular Cerebral/diagnóstico , Acidente Vascular Cerebral/epidemiologia , Acidente Vascular Cerebral/complicações , Fatores de Risco , Imageamento por Ressonância Magnética/métodos
4.
Chempluschem ; 87(11): e202200325, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36410784

RESUMO

The electrochemical aptamer-based (E-AB) biosensor usually has a long reaction time when detecting thrombin. This work reports the design of an E-AB biosensor with dual recognition sites to quickly detect thrombin. Specifically, two specific recognition sites of thrombin were used to design three aptamer sequences (TBA-15, TBA-29 and TBA-U), followed by fabrication of corresponding sensors. First, we tested these three types of biosensors in tris buffer solution, and found that the response time of the TBA-U sensor to the same concentration of thrombin was about 2 hours, which is shorter than TBA-15 and TBA-29 sensors. Then, we also did the same test in 50 % diluted serum with 500 nM thrombin. The response time of the TBA-U sensor was about 2 hours, which is still faster than the 3 hours of TBA-15 sensor and the 5.5 hours for TBA-29 sensor. In addition, in terms of dynamic range and specificity, TBA-U has good performance.


Assuntos
Técnicas Biossensoriais , Trombina , Soro , Oligonucleotídeos , Trometamina
5.
Cell Mol Immunol ; 2022 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-36348078

RESUMO

Psoriasis is a common chronic inflammatory skin disease. The diversity and heterogeneity of immune cells in human skin have been studied in recent years, but the spatial distribution of immune cells at the single-cell level in the human psoriatic epidermis and dermis remains unclear. In this study, we mapped psoriatic skin immune cells from paired lesional, perilesional, and nonlesional skin samples using mass cytometry. Phenotypic dendritic cells (DCs) were found in the psoriatic epidermis and dermis. Psoriatic dermal CD1c+CD11b+ cDC2s migrated to the epidermis in the perilesional skin during the preinitiation stage. CD1c+CD11b+ cDC2s rapidly replaced EpCAM+CD11clow LC cells and initiated inflammation. Simultaneously, CD207+CD11chi LC and CD5+ T cells accumulated in the psoriatic epidermis and orchestrated epidermal inflammation in psoriasis. The immune cell pool in the psoriatic dermis primarily included APCs and T cells. However, unlike that in the dermis, the epidermal immune environment was more significant and coincided with the inflammation occurring during psoriasis.The epidermal immune microenvironment plays a dominant role in psoriasis. Langerhans cells, epidermis-resident memory T cells and macrophages together contribute to healthy epidermal immune homeostasis. However, psoriatic CD1c+CD11b+ epidermal cDC2s are positioned in the perilesional area, replacing EpCAM+CD11clow LCs rapidly and initiating inflammation. Epidermal CD141+ cDC1s, CD1c+ cDC2s, CD14+ moDCs, and BDCA2+ pDCs orchestrate psoriatic inflammation. Meanwhile, CD11chi LCs and CD5+ T cells accumulate in the psoriatic epidermis.

6.
Front Neurol ; 13: 1018362, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36388198

RESUMO

Purpose: Parkinson's disease (PD) is a serious neurodegenerative disease affecting the elderly. In general, the locomotion deficit, which seriously affects the daily life of patients with PD, usually occurs at a later stage. The mask face symptom meanwhile progressively worsens. However, facial muscle disorders and changes involved in the freezing mask are unclear. Method: In this study, we recruited 35 patients with PD and 26 age- and sex-balanced controls to undergo phonation tests, while the built-in camera on the laptop recorded their facial expressions during the whole pronunciation process. Furthermore, FaceReader (version 7.0; Noldus Information Technology, Wageningen, Netherlands) was used to analyze changes in PD facial landmark movement and region movement. Results: The two-tailed Student's t-test showed that the changes in facial landmark movement among 49 landmarks were significantly lower in patients with PD than in the control group (P < 0.05). The data on facial region movement revealed that the eyes and upper lip of patients with PD differed significantly from those in the control group. Conclusion: Patients with PD had defects in facial landmark movement and regional movement when producing a single syllable, double syllable, and multiple syllables, which may be related to reduced facial expressions in patients with PD.

7.
Brain Sci ; 12(11)2022 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-36421858

RESUMO

It is commonly believed that alertness and attention decrease after sleep deprivation (SD). However, there are not enough studies on the changes in psychomotor vigilance testing (PVT) during SD and the corresponding changes in brain function and brain structure after SD. Therefore, we recruited 30 healthy adult men to perform a 36 h acute SD experiment, including the measurement of five indicators of PVT every 2 h, and analysis of cerebral blood flow (CBF) and grey matter volume (GMV) changes, before and after SD by magnetic resonance imaging (MRI). The PVT measurement found that the mean reaction time (RT), fastest 10% RT, minor lapses, and false starts all increased progressively within 20 h of SD, except for major lapses. Subsequently, all indexes showed a significant lengthening or increasing trend, and the peak value was in the range of 24 h-32 h and decreased at 36 h, in which the number of major lapses returned to normal. MRI showed that CBF decreased in the left orbital part of the superior frontal gyrus, the left of the rolandic operculum, the left triangular part, and the right opercular part of the inferior frontal gyrus, and CBF increased in the left lingual gyrus and the right superior gyrus after 36 h SD. The left lingual gyrus was negatively correlated with the major lapses, and both the inferior frontal gyrus and the superior frontal gyrus were positively correlated with the false starts. Still, there was no significant change in GMV. Therefore, we believe that 36 h of acute SD causes alterations in brain function and reduces alert attention, whereas short-term acute SD does not cause changes in brain structure.

8.
Adv Sci (Weinh) ; : e2204885, 2022 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-36382558

RESUMO

Methylating RNA post-transcriptionally is emerging as a significant mechanism of gene regulation in eukaryotes. The crosstalk between RNA methylation and histone modification is critical for chromatin state and gene expression in mammals. However, it is not well understood mechanistically in plants. Here, the authors report a genome-wide correlation between RNA 5-cytosine methylation (m5 C) and histone 3 lysine27 trimethylation (H3K27me3) in Arabidopsis. The plant-specific Polycomb group (PcG) protein EMBRYONIC FLOWER1 (EMF1) plays dual roles as activators or repressors. Transcriptome-wide RNA m5 C profiling revealed that m5 C peaks are mostly enriched in chromatin regions that lacked H3K27me3 in both wild type and emf1 mutants. EMF1 repressed the expression of m5 C methyltransferase tRNA specific methyltransferase 4B (TRM4B) through H3K4me3, independent of PcG-mediated H3K27me3 mechanism. The 5-Cytosine methylation on targets is increased in emf1 mutants, thereby decreased the mRNA transcripts of photosynthesis and chloroplast genes. In addition, impairing EMF1 activity reduced H3K27me3 levels of PcG targets, such as starch genes, which are de-repressed in emf1 mutants. Both EMF1-mediated promotion and repression of gene activities via m5 C and H3K27me3 are required for normal vegetative growth. Collectively, t study reveals a previously undescribed epigenetic mechanism of RNA m5 C modifications and histone modifications to regulate gene expression in eukaryotes.

9.
Vet Microbiol ; 275: 109594, 2022 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-36332300

RESUMO

Senecavirus A (SVA) is a newly emerging etiological agent of vesicular disease associated with sow abortion and acute piglet death, causing devastating economic consequences to global pig industry. IFN-induced protein with tetratricopeptide repeats (IFIT) genes are versatile in combating a variety of viruses, but the detailed mechanisms-of-action against SVA is unexplored. Transcriptomic analysis and immunoblot revealed high abundance of IFIT transcripts and proteins following SVA infection, initially implying the correlation between IFITs and SVA. Type I IFNs restricted SVA replication accompanied with substantial elevation of IFIT expression, potentializing IFITs as anti-SVA effectors downstream of IFN signaling. Gain-of-function assay demonstrated that IFIT3 rather than IFIT1/2 potently inhibited SVA replication, which was consistently verified by SVA strain SVV CH-FJ-2017 by TCID50 titration and an eGFP-tagged recombinant SVA using fluorescent microscopy. Afterwards, IFIT3 disrupted SVA life cycle at the early stage of virus binding and internalization, and at the late stage of virus assembly and release, as quantified by copy number and transmission electron microscopy, respectively. Directly transfecting SVA infectious RNA in IFIT3-overexpressed cells bypassed its antiviral activity, further suggesting that IFIT3 targeted viral life cycle beyond RNA replication. Further investigations showed that IFIT3 overexpression was incapable of regulating host immune responses against pathogens. Those results identified IFIT3 as a potent inhibitor of SVA and implicated IFIT3 pathway in the regulation of virus entry/assembly. In short, IFIT3 exerted significant inhibitory effects on the replication and spread of SVA, and played different functions in the life cycle of SVA.

10.
Rev. esp. enferm. dig ; 114(11): 648-653, noviembre 2022. ilus, tab, graf
Artigo em Inglês | IBECS | ID: ibc-JHG-1106

RESUMO

Objective: this study assessed the accuracy of linear endoscopic ultrasound (EUS) to diagnose submucosal (SM)invasion and compared linear EUS with miniprobe EUS insuspected early gastric cancer (EGC) patients.Methods: patients diagnosed with biopsy-verified suspected EGC were analyzed retrospectively. All cases wereexamined by linear EUS or miniprobe EUS for preoperativediagnosis of invasion depth and underwent endoscopic orsurgical treatment for radical resection. The invasion depthevaluated by EUS and pathology were categorized as noinvasion of SM and invasion of SM or deeper. The diagnosis of EUS was compared with postoperative pathologyresults.Results: a total of 105 patients were included in the finalanalysis. The overall prediction accuracy of linear EUS(n = 57) for SM invasion in suspected EGC was higherthan that of miniprobe EUS (n = 48), although no statistically significant differences were noted (82.5 % vs 72.9 %,p = 0.344). The negative predictive value (NPV) of linear EUSwas significantly higher than that of miniprobe EUS (100 %vs 82.8 %, p = 0.037). The binary logistic regression analysis showed that tumor size (p = 0.036), the presence of ulceration (p < 0.001) and EUS type (p = 0.027) were independentrisk factors for the diagnosis of SM invasion by EUS. Thearea under the receiver operating curve (ROC) was 0.889and 0.719 for linear and miniprobe EUS, respectively.Conclusion: linear EUS diagnosed suspected EGC for SMinvasion with a higher accuracy than miniprobe EUS. Inaddition, large and ulcerative lesions may lead to overestimation. (AU)


Assuntos
Humanos , Neoplasias , Endossonografia/métodos , Estadiamento de Neoplasias , Neoplasias Gástricas/patologia , Estudos Retrospectivos
11.
RSC Adv ; 12(44): 28576-28579, 2022 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-36320497

RESUMO

A convenient and efficient annulation reaction has been developed for the general synthesis of dinitrile-substituted cyclopropanes in moderate to excellent yields. A variety of 2-arylacetonitriles and α-bromoennitriles were compatible under the standard conditions. The reaction was achieved through tandem Michael-type addition followed by intramolecular cyclization. The preliminary application of this method was confirmed by the synthesis of the 2,4-dioxo-3-azabicyclo[3.1.0]hexane scaffold.

12.
Micromachines (Basel) ; 13(11)2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36422409

RESUMO

Implanting rotary blood pumps (RBPs) has become the principal treatment for patients suffering from severe heart failure. There are still many challenges to address for RBP control systems. These problems include meeting the patient's physiological perfusion, eliminating postoperative complications, as well as debugging the patient's physiological control system (automatically and indiscriminately). This paper proposes a non-invasive adaptive control system based on the Frank-Starling-like mechanism (NAC-FSL) to solve these problems. This control system uses the motor speed of the rotary blood pump as the only input variable, and the pump flow was estimated by the motor speed for achieving non-invasive detection. Simultaneously, a cardiovascular reference model was developed to provide an appropriate real-time preload for heart failure patients. The Frank-Starling-like control baseline was tracked to obtain the desired reference average pump flow by using the preload. Avoiding suction was done by adopting the control baseline (CLn), which included a flat slope under a high preload. Moreover, the NAC-FSL system could potentially unload the left ventricle and provide a higher pump flow with a smaller error during the exercise state, as compared to the CSC system. Finally, the K value indicating the preload sensitivity in the NAC-FSL controller was optimized to meet the perfusion needs according to the hemodynamic parameters.

13.
Cell Commun Signal ; 20(1): 185, 2022 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-36419191

RESUMO

BACKGROUND: The Isoleucyl-tRNA synthetase (IARS) catalyzes isoleucine to the corresponding tRNA, maintaining the accuracy of gene translation. Its role in psoriasis has been not investigated so far. In this study, we aimed to investigate the mechanisms underlying the efficacy of IARS inhibitor, mupirocin, treatment for psoriasis. METHODS: The expression of IARS was determined by immunofluorescence, Western blot and qRT-PCR in normal healthy control- and psoriatic human skin. An imiquimod (IMQ) -induced psoriasis-like skin disease model was used to study the phenotypes changed by an IARS inhibitor, mupirocin (MUP). Endotypes were analyzed by RNA-seq, R&D Luminex multi-factor technique, ELISA, immunofluorescence and flow cytometry. Additionally, the effect of MUP on epidermal keratinocytes (KCs) were conducted in-vitro in primary cultured human KCs. RESULTS: We found the expression of IARS was higher in psoriatic skin than in healthy controls. In IMQ-induced psoriasis-like C57BL/6 J mouse model, MUP reversed IMQ-induced keratinocytes proliferation, expression of inflammatory cytokines and infiltration of immune cells. Furthermore, in cultured human keratinocytes, MUP inhibited proliferation, but promoted apoptosis, which may be related with STAT3 signaling pathway. CONCLUSION: Our finding of blocking the infiltration of immune cells by inhibiting the formation of IARS, could be one mechanism to explain the effect of MUP in the treatment of psoriasis. Developing strategies targeting suppression IARS should open new perspectives for the treatment of psoriasis. Video Abstract.


Assuntos
Psoríase , Dermatopatias , Camundongos , Humanos , Animais , Imiquimode/efeitos adversos , Camundongos Endogâmicos C57BL , Isoleucina-tRNA Ligase , Mupirocina , Psoríase/induzido quimicamente , Psoríase/tratamento farmacológico
14.
Front Psychiatry ; 13: 1003261, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36424995

RESUMO

Objectives: To estimate the prevalence of depression symptoms and quality of life (QoL) and examined the influence of factors in the empty nest elderly. Methods: This was a cross-sectional study, which was conducted from February 2022 to May 2022. We recruited a convenience sample of no empty-nest elderly and empty-nest elderly (≥60 years) living in Chengdu. QoL was assessed using WHOQOL-BREF, Geriatric Depression Scale (GDS-15) was used to assess depression symptoms. Multivariable logistic regression was used to analyze data between independent variables with depression symptoms. Results: Two thousand twenty-six participants were included in this study, 39.0% (660/1,082) experienced depression symptoms among empty-nest elderly. Age (aOR, 1.02; 95% CI, 1.00-1.04; P = 0.046), chronic disease≥2 (aOR, 3.29; 95% CI, 2.50-4.33; P < 0.001) were associated with increased risk of depression symptoms, and physical activity (aOR, 0.59; 95% CI, 0.40-0.87; P = 0.008), physical health (aOR, 0.93; 95% CI, 0.88-0.99; P = 0.026), psychological health (aOR, 0.93; 95% CI, 0.87-0.98; P = 0.013), and total score (aOR, 0.97; 95% CI, 0.96-0.99; P < 0.001) were associated with decreased risk of depression symptoms among empty-nest elderly. Conclusions: Depression symptoms are common mental health problems among empty-nest elderly. We found that age, chronic disease ≥2 and physical activity were important factors that have an impact on depressive symptoms. Empty-nest elderly would have lowered QoL score.

15.
Front Surg ; 9: 1005898, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36425892

RESUMO

Objective: To compare the return to work (RTW) time between single-port laparoscopic surgery (SPLS) and multiport laparoscopic surgery (MPLS) for benign ovarian tumors. Methods: A cross-sectional cohort study was conducted, which consisted of 335 women of reproductive age with benign ovarian tumors and who were keen on returning to work as early as possible. Surgical outcomes, postoperative pain score, postoperative satisfaction with the cosmesis score (CS), and the RTW time of the SPLS group were compared with those of the MPLS group. Besides, the RTW time and CS were calculated from the questionnaire survey by a single specialized gynecologist. Results: Women who met the inclusion criteria were included in the SPLS (n = 106) and MPLS groups (n = 229). The RTW time in the SPLS group (22.13 ± 27. 06 days) was significantly shorter than that in the MPLS group (46.08 ± 57.86 days) (P < 0.001). The multivariate Cox analysis results showed that age (HR = 0.984, 95% CI, 0.971-0.997, P = 0.020), SPLS (HR = 3.491, 95% CI, 2.422-5. 032, P < 0.001), and return to normal activity time (HR = 0.980, 95% CI, 0.961-0.998, P = 0.029) were independent factors of the RTW time. Conclusions: SPLS may be advantageous in terms of shortening the RTW time for women with benign ovarian tumors.

16.
Front Genet ; 13: 987519, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36212120

RESUMO

Plasma homocysteine (Hcy) has been identified as a potential risk factor for cerebral small vessel disease. Cerebral small vessel disease (CSVD) leads to cognitive impairment, depression, and other symptoms and is a common disease in middle-aged and elderly people. To investigate the relationship between 5,10-methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism and CSVD in elderly patients, plasma levels of homocysteine (Hcy) and MTHFR genotyping were assessed. MRI and MRA were performed at the same time to analyze the relationship between different genotypes and cerebrovascular lesions. We showed that Hcy plasma levels in the TT group were significantly higher than those in the CC and CT groups. Moreover, we observed that the severity of white matter lesions was associated with women and positively correlated with age, previous coronary heart disease, luminal infarction, and MTHFR polymorphism. The multivariate logistic regression analysis showed that age, TT genotype, and lacunar infarction were independent risk factors for white matter hyperintensity (WMH). Importantly, we showed that there was a significant correlation between Hcy plasma levels and MTHFR gene polymorphism, with the TT genotype constituting an independent risk factor for WMH. Therefore, we recommended early detection of MTHFR gene polymorphisms with concomitant early intervention concerning risk factors to delay the occurrence of cognitive impairment in CSVD elderly patients.

17.
Adv Mater ; : e2204413, 2022 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-36239270

RESUMO

Photoresponsive supramolecular polymers are well-organized assemblies based on highly oriented and reversible non-covalent interactions containing photosensitive molecules as (co-)monomers. They have attracted increasing interest in smart materials and dynamic systems with precisely controllable functions, such as light-driven soft actuators, photoresponsive fluorescent anti-counterfeiting, and light-triggered electronic devices. The present review discusses light-activated molecules used in photoresponsive supramolecular polymers with their main photo-induced changes, e.g., geometry, dipole moment, and chirality. Based on these distinct changes, supramolecular polymers formed by light-activated molecules exhibit photoresponsive disassembly and re-assembly. As a consequence, photo-induced supramolecular polymerization, "depolymerization," and regulation of the lengths and topologies are observed. Moreover, the light-controlled functions of supramolecular polymers, such as actuation, emission, and chirality transfer along length scales, are highlighted. Furthermore, a perspective on challenges and future opportunities is presented. Besides the challenge of moving from harmful UV-light to visible /near IR light avoiding fatigue, and enabling biomedical applications, future opportunities included light-controlled supramolecular actuators with helical motion, light-modulated information transmission, optically recyclable materials, and multi-stimuli responsive supramolecular systems. This article is protected by copyright. All rights reserved.

18.
Heliyon ; 8(9): e10691, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36185154

RESUMO

Globally all countries encounter air pollution problems along their development path. As a significant indicator of air quality, PM2.5 concentration has long been proven to be affecting the population's death rate. Machine learning algorithms proven to outperform traditional statistical approaches are widely used in air pollution prediction. However research on the model selection discussion and environmental interpretation of model prediction results is still scarce and urgently needed to lead the policy making on air pollution control. Our research compared four types of machine learning algorisms LinearSVR, K-Nearest Neighbor, Lasso regression, Gradient boosting by looking into their performance in predicting PM2.5 concentrations among different cities and seasons. The results show that the machine learning model is able to forecast the next day PM2.5 concentration based on the previous five days' data with better accuracy. The comparative experiments show that based on city level the Gradient Boosting prediction model has better prediction performance with mean absolute error (MAE) of 9 ug/m3 and root mean square error (RMSE) of 10.25-16.76 ug/m3, lower compared with the other three models, and based on season level four models have the best prediction performances in winter time and the worst in summer time. And more importantly the demonstration of models' different performances in each city and each season is of great significance in environmental policy implications.

19.
Front Genet ; 13: 848985, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36186459

RESUMO

Th17 and regulatory T cells (Tregs) play crucial roles in the pathogenesis of autoimmune diseases. Th17/Treg homeostasis is critically involved in maintaining the immune balance. Disturbed Th17/Treg homeostasis contributes to the progression of autoimmune diseases. MicroRNAs (miRNAs) have emerged as a new vital factor in the regulation of disturbed Th17/Treg homeostasis. To better understand the epigenetic mechanisms of miRNAs in regulating Treg/Th17 homeostasis, we included and evaluated 97 articles about autoimmune diseases and found that miRNAs were involved in the regulation of Treg/Th17 homeostasis from several aspects positively or negatively, including Treg differentiation and development, Treg induction, Treg stability, Th17 differentiation, and Treg function. Uveitis is one of the ocular autoimmune diseases, which is also characterized with Th17/Treg imbalance. However, our understanding of the miRNAs in the pathogenesis of uveitis is elusive and not well-studied. In this review, we further summarized miRNAs found to be involved in autoimmune uveitis and their potential role in the regulation of Th17/Treg homeostasis.

20.
Physiol Plant ; : e13797, 2022 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-36251672

RESUMO

In many higher plants, fatty acid (FA) biosynthesis is coordinately regulated at multiple levels by intricate regulatory networks. However, the factors and their regulatory mechanisms underlying seed oil accumulation are still limited. Here, we identified that loss of glycolytic metalloenzyme enolase2 (AtENO2) activity increased the contents of total FAs and salicylic acid (SA) but reduced the accumulation of flavonoids and mucilage by regulating the expression of key genes involved in their biosynthesis pathway in Arabidopsis thaliana seeds. AtENO2 physically interacts with the transcription factor AtTGA5, which may participate in the regulation of SA levels. Non-targeted metabolomics analysis of eno2- and WT also showed that the levels of three flavonoids, quercetin-3-galactoside, quercitrin and epicatechin, were significantly decreased in eno2- , and the flavonoid biosynthesis pathway was also enriched in the KEGG analysis. Meanwhile, the mutation of AtENO2 delayed silique ripening, thereby prolonging silique photosynthesis time, allowing siliques to generate more photosynthesis products for FA biosynthesis. These results reveal a molecular mechanism by AtENO2 to regulate seed oil accumulation in A. thaliana, providing potential targets for improving crop seed oil quality. This article is protected by copyright. All rights reserved.

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