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1.
Signal Transduct Target Ther ; 7(1): 312, 2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36071039

RESUMO

The Coronavirus Disease 2019 (COVID-19) caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a global pandemic that seriously threatens health and socioeconomic development, but the existed antiviral drugs and vaccines still cannot yet halt the spread of the epidemic. Therefore, a comprehensive and profound understanding of the pathogenesis of SARS-CoV-2 is urgently needed to explore effective therapeutic targets. Here, we conducted a multiomics study of SARS-CoV-2-infected lung epithelial cells, including transcriptomic, proteomic, and ubiquitinomic. Multiomics analysis showed that SARS-CoV-2-infected lung epithelial cells activated strong innate immune response, including interferon and inflammatory responses. Ubiquitinomic further reveals the underlying mechanism of SARS-CoV-2 disrupting the host innate immune response. In addition, SARS-CoV-2 proteins were found to be ubiquitinated during infection despite the fact that SARS-CoV-2 itself didn't code any E3 ligase, and that ubiquitination at three sites on the Spike protein could significantly enhance viral infection. Further screening of the E3 ubiquitin ligases and deubiquitinating enzymes (DUBs) library revealed four E3 ligases influencing SARS-CoV-2 infection, thus providing several new antiviral targets. This multiomics combined with high-throughput screening study reveals that SARS-CoV-2 not only modulates innate immunity, but also promotes viral infection, by hijacking ubiquitination-specific processes, highlighting potential antiviral and anti-inflammation targets.


Assuntos
COVID-19 , SARS-CoV-2 , Antivirais , Humanos , Proteômica , Ubiquitina-Proteína Ligases , Ubiquitinação/genética
2.
Mol Plant ; 2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36071671

RESUMO

Evolution is driven by various mechanisms. Directional increase in embryo to endosperm ratio is an evolutionary trend within angiosperms. Endosperm constitutes a major seed volume of Poales and some dicots. In other dicots such as Arabidopsis and soybean, endosperm proliferates early followed by embryo growth to replace the endosperm. Arabidopsis Leucine-rich repeat receptor protein kinase AtHAIKU2 (AtIKU2) is a key regulator of early endosperm proliferation. IKU2s from Brachypodium, rice and soybean complement Atiku2 phenotype. AtIKU2 also rescues endosperm proliferation in Brachypodium BdIKU2 knockout. AtIKU2 and soybean GmIKU2 are actively expressed in a few days after fertilization. Thereafter, AtIKU2 is suppressed by FIS-PRC2 and GmIKU2 locus is enriched with H3K27me3 marks. Histone methyltransferase AtMEA is unique to Brassicaceae, but one GmSWN is an early version of AtMEA. In contrast, the BdIKU2 and rice OsIKU2 loci sustain a continuous expression and are devoid of H3K27me3 marks. IKU2 retains an ancestral function. Its expression duration contributes to silenced or persistent endosperm proliferation. We reveal an epigenetic mechanism that drives the development of vastly different seed ontogeny.

3.
Inflammopharmacology ; 2022 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-36085400

RESUMO

INTRODUCTION: This study investigated the effects of soluble epoxide hydrolase (sEH) inhibitors on acute lung injury (ALI) using the measure of meta-analysis. METHODS: Relative publications were systematic reviewed and retrieved by searching electronic databases including the Cochrane Library, PubMed, China National Knowledge Infrastructure, Wanfang Data, and Google Scholar. RESULTS: Seven animal studies were included in this meta-analysis. Our result showed that the lung injury scores (SMD = - 2.31, 95% CI - 3.50 to - 1.12) and lung wet to dry weight ratios (WMD-1.44, 95% CI - 1.69 to - 1.18) were reduced in sEH inhibitors-treated animals compared with control. The mortality was improved by sEH inhibitors at 48 h (RR = 0.62, 95% CI 0.42 to 0.92), 72 h, and 120 h, but not at 24 h (RR = 0.59, 95% CI 0.35 to 1.01) and 96 h, after induction of ALI model. CONCLUSIONS: The sEH inhibitor is a potent candidate of pharmacological agents for ALI/acute respiratory distress syndrome, as its effects on improvement of lung injury and mortality in preclinical researches.

5.
Artigo em Inglês | MEDLINE | ID: mdl-36068192

RESUMO

The layer-by-layer liquid-phase epitaxy (LBL-LPE) method is widely used in preparing metal-organic framework (MOF) thin films with merits of controlling thickness and out-of-plane orientation for superior performances in applications. The LBL-LPE growth mechanism related to the grain boundary, structure defect, and orientation is critical but very challenging to study. In this work, a novel "in-plane self-limiting and self-repairing" thin-film growth mechanism is demonstrated by the combination study of the grain boundary, structure defect, and orientation of Cu3(HHTP)2-xC thin film via microscopic analysis techniques and electrical measurements. This mechanism results a desired high-quality MOF thin film with preferred in-plane orientation at its bottom for the first time, and is very helpful for optimizing the LBL-LPE method, understanding the growth cycle-dependent properties of MOF thin film, and inspiring the investigations of the biomimetic self-repairing materials.

6.
Acta Neurol Belg ; 2022 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-36097211

RESUMO

OBJECTIVES: To explore the association between cerebral small vessel disease (cSVD) and cognitive impairment (CI) in Parkinson's disease (PD). METHODS: 81 PD patients were recruited into the study from September 2018 to December 2020. The demographic characteristics and radiologic and laboratory data were collected. Cognitive assessments were carried out using the Montreal Cognitive Assessment. The association between cSVD and cognitive impairment was analyzed using univariate and binary logistic regression analysis. RESULTS: The binary logistic regression analysis showed that, after correcting for age, educational years, hyperhomocysteinemia, hypertension, and diabetes mellitus, total cSVD scores (OR 1.55, 95% CI 1.07-2.27, P = 0.02), the presence of paraventricular white matter hyperintensity (PVH) (OR 11.78, 95% CI 3.08-45.01, P < 0.001), white matter hyperintensity (WMH) (OR 7.95, 95% CI 2.28-27.79, P = 0.001), and perivascular space (PVS) (OR 6.66, 95% CI 2.08-21.40, P = 0.001) were independent risk factors for PD-CI. CONCLUSION: The presence of cSVD was associated with cognitive dysfunction in patients with PD. It may be beneficial to manage cSVD to prevent the progression of cognitive impairment in patients with PD.

7.
Surg Oncol ; 44: 101849, 2022 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-36116415

RESUMO

INTRODUCTION: The prognosis of patients with pancreatic ductal adenocarcinoma (PDAC) is highly variable and there is a paucity of effective treatment options for patients with PDAC. Genome-wide analyses may allow for potential drugs to be identified using differentially expressed genes, as well as constructing protein interaction networks and molecule-gene connectivity mapping. METHODS: Microarray data of RNA expression profiling of PDAC and normal pancreas tissues were downloaded from the Gene Expression Omnibus (GEO). Functional and pathway enrichment information of the DEGs was obtained using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases. Corresponding homologous proteins were analyzed by protein-protein interaction analysis. Survival-related hub genes were screened and potential therapeutic drugs for PDAC were identified using the connectivity mapping (cMap). RESULTS: Of 18,229 PDAC genes assessed using RNA expression profiling from 118 PDAC tumor samples and 13 normal pancreatic tissue samples, 1502 and 744 genes were upregulated and downregulated, respectively, versus normal pancreas tissue. Protein-protein interaction analysis revealed 10 upregulated hub genes (ITGB1, ITGAV, SDC1, KRAS, CCNB2, COL1A2, AURKA, CDC20, COL1A1, COL3A1) and 10 downregulated hub genes (CPB1, CPA1, CPA2, CTRB2, CTRC, CELA3A, CELA2B, PRSS3, CELA2A, REG1A). The connectivity mapping score related to this hub gene list was used to generate the candidate drugs for PDAC treatment, which includes tyrosine kinase inhibitors (lucitanib, lapatinib, ceritinib and CYT-387), serine/threonine protein kinase inhibitors (roscovitine, BS-181, purvalanol-a, MK-2206 and palomid-529) and other small molecules. CONCLUSION: Using available genetic atlas data, potential drug candidates for treatment of PDAC were identified based on differentially expressed genes, protein interaction analysis and connectivity mapping. These results may help focus efforts on identifying targeted agents with potential therapeutic efficacy for evaluation in prospective clinical trials of patients with PDAC.

8.
J Exp Clin Cancer Res ; 41(1): 270, 2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36071454

RESUMO

BACKGROUND: Long noncoding RNAs (lncRNAs) are emerging as critical regulators of gene expression and play fundamental roles in various types of cancer. Current developments in transcriptome analyses unveiled the existence of lncRNAs; however, their functional characterization remains a challenge. METHODS: A bioinformatics screen was performed by integration of multiple omics data in hepatocellular carcinoma (HCC) prioritizing a novel oncogenic lncRNA, LINC01132. Expression of LINC01132 in HCC and control tissues was validated by qRT-PCR. Cell viability and migration activity was examined by MTT and transwell assays. Finally, our results were confirmed in vivo mouse model and ex vivo patient derived tumor xenograft experiments to determine the mechanism of action and explore LINC01132-targeted immunotherapy. RESULTS: Systematic investigation of lncRNAs genome-wide expression patterns revealed LINC01132 as an oncogene in HCC. LINC01132 is significantly overexpressed in tumor and associated with poor overall survival of HCC patients, which is mainly driven by copy number amplification. Functionally, LINC01132 overexpression promoted cell growth, proliferation, invasion and metastasis in vitro and in vivo. Mechanistically, LINC01132 acts as an oncogenic driver by physically interacting with NRF and enhancing the expression of DPP4. Notably, LINC01132 silencing triggers CD8+ T cells infiltration, and LINC01132 knockdown combined with anti-PDL1 treatment improves antitumor immunity, which may prove a new combination therapy in HCC. CONCLUSIONS: LINC01132 functions as an oncogenic driver that induces HCC development via the NRF1/DPP4 axis. Silencing LINC01132 may enhance the efficacy of anti-PDL1 immunotherapy in HCC patients.


Assuntos
Carcinoma Hepatocelular , Dipeptidil Peptidase 4 , Neoplasias Hepáticas , Fator 1 Nuclear Respiratório , RNA Longo não Codificante , Animais , Carcinogênese/genética , Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/terapia , Linhagem Celular Tumoral , Dipeptidil Peptidase 4/genética , Regulação Neoplásica da Expressão Gênica , Humanos , Terapia de Imunossupressão , Neoplasias Hepáticas/patologia , Camundongos , Fator 1 Nuclear Respiratório/genética , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismo
9.
J Clin Med ; 11(17)2022 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-36079059

RESUMO

BACKGROUND: There is accumulating evidence indicating that inflammation and oxidative stress are involved in the pathogenesis of atrial fibrillation (AF). The role of manganese superoxide dismutase (MnSOD) in the initiation and maintenance of AF has not yet been well characterized. The aim of our study is to investigate whether or not plasma MnSOD levels are associated with AF. METHODS: We enrolled a total of 130 consecutive patients with AF as the case group (paroxysmal AF: 87, persistent AF: 43) and 58 patients without a history of AF as the control group after screening. Baseline clinical characteristics, laboratory and echocardiographic parameters were collected. Plasma levels of nicotinamide-adenine dinucleotide phosphate oxidase 4 (NOX4) and MnSOD were measured by an enzyme-linked immunosorbent assay (ELISA) method. These data were compared between the different groups. The relationship between MnSOD and other parameters was assessed using Spearman correlation. Multivariable logistic regression analysis was performed to identify independent predictors of AF. The area under the curve (AUC) from receiver operating characteristics (ROC) analysis was constructed to explore the value of MnSOD in predicting the occurrence of AF. RESULTS: The levels of MnSOD were the highest in the paroxysmal AF group, followed by the persistent AF group, and the lowest in the controls. Meanwhile, the levels in the paroxysmal AF group were significantly higher than those in the controls [322.84 (165.46, 547.61) vs. 201.83 (129.53, 301.93), p = 0.002], but no significant difference was found between the paroxysmal AF group and persistent AF group, as well as the persistent AF group and the controls. Spearman correlation analysis indicated that there was a significantly negative correlation between MnSOD levels and LAD (r = -0.232, p = 0.008) and a positive correlation between MnSOD levels and RDW-CV (r = 0.214, p = 0.014) in the case group. Multivariate logistic regression analysis indicated that MnSOD levels [odds ratio (OR): 1.003, 95% confidence interval (CI): 1.001-1.005, p = 0.002] were an independent risk factor for paroxysmal AF, and the best cut-off value of MnSOD in predicting paroxysmal AF gained by ROC curve analysis was 311.49 ug/mL (sensitivity of 52.9%, specificity of 77.6%, AUC = 0.668). CONCLUSION: Oxidative stress underlies the pathogenesis of AF and may play a stronger role in paroxysmal AF than persistent AF. Our study showed an independent association between increased circulating plasma MnSOD levels and the occurrence of paroxysmal AF.

10.
Front Physiol ; 13: 979367, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36105293

RESUMO

The purpose of this study was to explore the effects of the chain squat training (CST) with different chain load ratio (0, 10%, 20% and 30%) on the explosive power of the lower limbs of adolescent male basketball players. Forty-four youth basketball players (age 15.48 ± 0.81 years, body mass 78.86 ± 12.04 kg, height 184.95 ± 6.71 cm) were randomly allocated to one of the four groups: traditional squat training (TST), 10% chains squat training (10% CST), 20% chains squat training (20% CST), and 30% chains squat training (30% CST). Training interventions were performed 2 times per week for 6 weeks, and at the week before (Pre) and after (Post) the 6-week CST program with different chain load ratio, the no-step vertical jump, standing long jump, 15 m shuttle run, 1 R M squat and 30 m sprint test were performed. A 4 (group) × 2 (time) repeated measures analysis of variances (ANOVA) was calculated to show the scatter of each variable, and the Bonferroni's post-hoc test was used for multiple comparisons, in addition the partial eta-squared (η2) was calculated as an estimate of the ES. Significant time × group interaction was noticed for the no-step vertical jump (p < 0.001; η2 = 0.611), standing long jump (p < 0.001; η2 = 0.490) and 1 R M squat (p < 0.01; η2 = 0.333) indicating that better improvements appear in CST compared to TST. However, significant time × group interaction was noted for 15 m shuttle run (p < 0.001; η2 = 0.428), in favor of TST compared to CST. In addition, the improvements in 30 m sprint were similar between all groups. In conclusion, CST with more chain load has better training effects on lower limb explosive strength and maximum strength, based on the improvement in 1 R M squat and jumping performance. Besides, compared with TST, CST with more chain load might not help to develop better velocity adaptation at higher range of movement.

11.
Front Neurol ; 13: 919866, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36081876

RESUMO

Purpose: To investigate the effect and safety of flow diverters in the treatment of unruptured dissecting intracranial aneurysms of the vertebral artery in comparison with stent-assisted coiling or stenting alone. Materials and methods: Patients with unruptured dissecting intracranial aneurysms of the vertebral artery treated with the flow diverter, stent-assisted coiling, or stenting alone were retrospectively enrolled. The clinical data were analyzed and compared. Results: Twenty-five patients were enrolled in the flow diversion group and 42 patients in the stenting group. Twenty-six flow diverters were deployed in the flow diversion group. Immediate angiography revealed contrast agent retention within the aneurysm cavity in all patients. In the stenting group, 48 stents were deployed, and immediate angiographic outcome showed O'Kelly-Marotta (OKM) grade D in 18 (42.9%) aneurysms, grade C in 16 (38.1%), and grade B in 8 (19.0%). Periprocedural ischemic complications of thrombosis occurred in two (4.8%) patients and were treated with thrombolysis. In the flow diversion group, 19 (76%) patients underwent angiographic follow-up 3-46 (median 24) months after the procedure, with the OKM grade D in 11 (57.9%) patients, C in two (10.5%), and B in six (31.6%). The aneurysm recurrence rate was zero, and all diverters remained patent. Asymptomatic instent stenosis occurred in two (10.5%) patients. In seven of the ten patients with mild or moderate parent artery stenosis before the procedure who experienced angiographic follow-up, the stenosis was improved in five (71.4%) patients. In the stenting group, angiographic follow-up was carried out in 33 (78.6%) patients 6-58 months (median 34) after the procedure, with OKM grade D in 22 (66.7%) patients, grade C in five (15.2%), grade B in three (9.1%), and aneurysm recurrence (grade B, with increased contrast agent into the aneurysm cavity) in three (9.1%). Five (16.7%) patients experienced asymptomatic instent stenosis, and six of the 12 patients (50%) with parent artery stenosis were improved. Conclusion: Flow diverters with or without selective adjunctive coiling for the treatment of unruptured dissecting intracranial aneurysms of the vertebral artery may be safe and effective with good occlusion effects not inferior to those of stent-assisted coiling and stenting alone even though the long-term effect still warrants confirmation.

12.
Micromachines (Basel) ; 13(8)2022 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-36014236

RESUMO

The two-stroke micro free piston swing engine (MFPSE) is a portable power device. Its seal performance plays an important role in the dynamic properties and efficiency of microengines. The present work established the leakage model of the two-stroke micro free piston swing engine by utilizing the compressible flow Reynolds Navier-Stokes equation. The obtained nondimensional mass leakage was related to the seal gap height, seal inlet pressure, size factor and compression ratio. Simulation investigated how the different seal gap heights and size factors affected the pressure, temperature and mass leakage of micro engines. The results showed that when the seal gap height of the combustion chamber increased, the maximum pressure and cycle power declined, obviously. However, the maximum temperature was scarcely affected. The mass leakage was not greatly impacted when the compression ratio was less than 5. However, the mass leakage dramatically increased when the seal gap was more than 10 µm or the size factor was less than 0.4. The investigation revealed these mass leakage effect factors and provided a guide for the seal and structure design of the two-stroke micro free piston swing engine.

13.
Nat Commun ; 13(1): 4955, 2022 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-36002452

RESUMO

Most intensive human activities occur in lowlands. However, sporadic reports indicate that human activities are expanding in some Asian highlands. Here we investigate the expansions of human activities in highlands and their effects over Asia from 2000 to 2020 by combining earth observation data and socioeconomic data. We find that ∼23% of human activity expansions occur in Asian highlands and ∼76% of these expansions in highlands comes from ecological lands, reaching 95% in Southeast Asia. The expansions of human activities in highlands intensify habitat fragmentation and result in large ecological costs in low and lower-middle income countries, and they also support Asian developments. We estimate that cultivated land net growth in the Asian highlands contributed approximately 54% in preventing the net loss of the total cultivated land. Moreover, the growth of highland artificial surfaces may provide living and working spaces for ∼40 million people. Our findings suggest that highland developments hold dual effects and provide new insight for regional sustainable developments.


Assuntos
Asiáticos , Ecossistema , Ásia , Ásia Sudeste , Humanos
14.
Artigo em Inglês | MEDLINE | ID: mdl-36018412

RESUMO

In this study, a recyclable Fe/S co-doped nanocarbon (Fe/S-NC) was successfully prepared by the pyrolysis of ZIF-8 confined with Fe(II) and added S. Characterization showed that a highly graphitized carbon-based material co-doped with sulfur and iron was successfully prepared. This Fe/S-NC can efficiently activate PMS to remove organic pollutants in water. The effect of different synthesis conditions on the degradation efficiency of 2,4-DCP was studied by orthogonal experiments. The optimized Fe/S-NC/PMS system exhibited excellent catalytic performance and could degrade more than 99.7% of 2,4-DCP within 30 min. Even after 5 cycles, the degradation efficiency could still be maintained above 96.3%, which proved that the catalytic system had good cycle performance. In addition, the effect of pH on catalytic performance showed that the degradation rate of 2,4-DCP exceeds 96.7% in the pH range of groundwater (pH = 5-9). We had confirmed that the free radicals that caused 2,4-DCP degradation were SO4·-, ·OH, O2·-, and 1O2, which played important roles in degrading organic pollutants. These research results show that the Fe/S-NC/PMS system can be used as an efficient, stable, and environmentally friendly system to treat organic pollutants in groundwater.

15.
Heliyon ; 8(8): e10073, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35991978

RESUMO

Type 1 diabetes mellitus (T1DM) is a type of diabetes caused by the destruction of pancreatic ß cells and the absolute lack of insulin secretion. T1DM usually starts in adolescence or develops directly as a severe disease state of ketoacidosis. T1DM and its complications make many people suffer and have psychological problems, which make us have to pay more attention to the prevention and early control of T1DM. Cognitive impairment (CI) is one of the major complications of T1DM. It can further develop into Alzheimer's disease, which can seriously affect the quality of life of the elderly. Furthermore, the relationship between T1DM and CI is unclear. Hence, we conducted a narrative review of the existing literature through a PubMed search. We summarized some risk factors that may be associated with the cognitive changes in T1DM patients, including onset age and duration, education and gender, glycemic states, microvascular complications, glycemic control, neuropsychology and emotion, intestinal flora, dyslipidemia, sleep quality. We aimed to provide some content related to CI in T1DM, and hoped that it could play a role in early prediction and treatment to reduce the prevalence.

16.
Brief Bioinform ; 2022 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-35959990

RESUMO

Protein side chains are vitally important to many biological processes such as protein-protein interaction. In this study, we evaluate the performance of our previous released side-chain modeling method OPUS-Mut, together with some other methods, on three oligomer datasets, CASP14 (11), CAMEO-Homo (65) and CAMEO-Hetero (21). The results show that OPUS-Mut outperforms other methods measured by all residues or by the interfacial residues. We also demonstrate our method on evaluating protein-protein docking pose on a dataset Oligomer-Dock (75) created using the top 10 predictions from ZDOCK 3.0.2. Our scoring function correctly identifies the native pose as the top-1 in 45 out of 75 targets. Different from traditional scoring functions, our method is based on the overall side-chain packing favorableness in accordance with the local packing environment. It emphasizes the significance of side chains and provides a new and effective scoring term for studying protein-protein interaction.

17.
Cell Death Discov ; 8(1): 353, 2022 Aug 08.
Artigo em Inglês | MEDLINE | ID: mdl-35941127

RESUMO

We explored the potential activity of compound 16 (Cpd16), a novel small molecule Nrf2 activator, in hydrogen peroxide (H2O2)-stimulated osteoblasts. In the primary murine/human osteoblasts and MC3T3-E1 murine osteoblastic cells, Cpd16 treatment at micro-molar concentrations caused disassociation of Keap1-Nrf2 and Nrf2 cascade activation. Cpd16 induced stabilization of Nrf2 protein and its nuclear translocation, thereby increasing the antioxidant response elements (ARE) reporter activity and Nrf2 response genes transcription in murine and human osteoblasts. Significantly, Cpd16 mitigated oxidative injury in H2O2-stimulited osteoblasts. H2O2-provoked apoptosis as well as programmed necrosis in osteoblasts were significantly alleviated by the novel Nrf2 activator. Cpd16-induced Nrf2 activation and osteoblasts protection were stronger than other known Nrf2 activators. Dexamethasone- and nicotine-caused oxidative stress and death in osteoblasts were attenuated by Cpd16 as well. Cpd16-induced osteoblast cytoprotection was abolished by Nrf2 short hairpin RNA or knockout, but was mimicked by Keap1 knockout. Keap1 Cys151S mutation abolished Cpd16-induced Nrf2 cascade activation and osteoblasts protection against H2O2. Importantly, weekly Cpd16 administration largely ameliorated trabecular bone loss in ovariectomy mice. Together, Cpd16 alleviates H2O2-induced oxidative stress and death in osteoblasts by activating Nrf2 cascade.

18.
J Mol Model ; 28(9): 255, 2022 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-35963954

RESUMO

Based on the first-principles density functional theory, the electronic structure and optical properties of CH3NH3HgI3 crystal were investigated by means of generalized gradient approximation (GGA + U) approach. Then, CH3NH3HgI3 crystal was grown successfully by anti-solvent method, and the UV-vis-NIR spectra of crystal grown were investigated. The results indicate that CH3NH3HgI3 belongs to direct band-gap semiconductor with 2.801 eV of band-gap. The energy level of electrons at the top of the valence band and the bottom of the conduction band is mainly formed by Hg and I, while C, N, and H of CH3NH3+ do not participate in forming the electron configuration near the Fermi level. CH3NH3HgI3 was combined by electrostatic interactions with CH3NH3+ and [HgI4]2-. Their electrostatic interactions lead to distortion of [HgI4]2- tetrahedron and cause the increase of band-gap of CH3NH3HgI3. Moreover, steric hindrance effect of CH3NH3+ spurs [HgI4]2- tetrahedron to form 1D chain structure. The result obtained from the UV-vis-NIR spectra of crystal shows that the band-gap of CH3NH3HgI3 crystal is 2.877 eV, which is good coincident with the calculated gap (2.801 eV). Our discussions on the electronic structural and energy band of crystal suggest that CH3NH3HgI3 can be used as an ultraviolet detector material.

19.
Dis Markers ; 2022: 1599112, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35968499

RESUMO

Objective: This study is aimed at exploring the clinical manifestations and prognostic factors of osteosarcoma. Methods: The clinical data of patients with osteosarcoma who were treated in our hospital from January 2018 to March 2020 were selected for retrospective analysis. The general information of the patients, including age, gender, tumor diameter, tumor location, tumor type, surgical method, and Enneking stage, distant disease metastasis, KPS score, and the number of postoperative adjuvant chemotherapy, were grouped by prognosis for statistical analysis. The clinical characteristics, morbidity and mortality, and prognostic factors of patients were statistically analyzed. Results: Among the 83 patients in this group, there were 52 males and 31 females, 59 tumors > 10 cm in diameter and 24 tumors < 10 cm in diameter, 16 tumors in the upper limbs and 67 tumors in the lower limbs, 25 tumors in osteoblastoma, 16 tumors in chondroblastoma, 42 tumors in fibroblastoma, 62 tumors in stage II, and 21 tumors in stage III of Enneking stage, 10 tumors in distant metastasis, and 10 tumors in distant metastasis. The death rate of this group was 19.28% (16/83). Multifactor regression analysis confirmed that the Enneking stage III, distant metastasis, KPS score < 70, and the number of postoperative adjuvant chemotherapy < 6 were important factors influencing the death of osteosarcoma (P < 0.05). Conclusion: Enneking stage III, distant metastasis, KPS score < 70, and the number of postoperative adjuvant chemotherapy < 6 are important influencing factors of osteosarcoma death. Clinical practice can take corresponding preventive and control measures according to the existence of these factors to ensure a good prognosis.


Assuntos
Neoplasias Ósseas , Osteossarcoma , Neoplasias Ósseas/patologia , Quimioterapia Adjuvante , Feminino , Humanos , Masculino , Osteossarcoma/patologia , Osteossarcoma/terapia , Prognóstico , Estudos Retrospectivos
20.
Bioorg Chem ; 128: 106059, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-35933895

RESUMO

Ten new icetexane diterpenoids, salpratins E-N (1-10) and a known analogue (11) were characterized from Salvia prattii Hemsl. Structurally, 1 is the first 19(4 â†’ 3)-abeo-icetexane diterpenoid featuring with a 6/7/6 ring system. The structures of isolated compounds were determined by comprehensive analyses of spectroscopic data, ECD calculation, and single-crystal X-ray diffraction. Biological studies initially revealed that 1, 7, 10, and 11 are notable Cav3.2 T-type Ca2+ channel (TTCC) inhibitors with IC50 values of 2.9, 5.1, 2.3, and 3.2 µM, respectively. Five icetexane related derivatives (13-17) were synthesized from an abietane type precursor, (+)-carnosic acid (12), for the purpose of overcoming the poor water solubility of aforementioned active compounds and further investigating diverse diterpenes with valuable activity. Among them, 13 and 14 showed potent inhibitions on Cav3.2, having IC50 values of 6.7 and 2.4 µM, respectively. Significantly, they exhibited dose-dependent (1, 3, and 10 mg/kg) and comparable analgesic effects as that of Z944, a TTCCs inhibitor under clinical trial for pain management, in the mouse acetic acid writhing test. These findings further enrich structural diversity and bioactivity of Salvia diterpenoids, as well as provide promising structural templates for the development of Cav3.2 analgesics.


Assuntos
Canais de Cálcio Tipo T , Diterpenos , Salvia , Analgésicos/química , Analgésicos/farmacologia , Analgésicos/uso terapêutico , Animais , Cristalografia por Raios X , Diterpenos/química , Diterpenos/farmacologia , Camundongos , Estrutura Molecular , Salvia/química
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