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1.
J Hazard Mater ; 393: 122458, 2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-32155526

RESUMO

As to metallosilicate zeolites, ions with larger size such as Ta5+ in the gels greatly retarded their crystallization during the hydrothermal synthesis, affording long-winded synthesis periods, up-limited framework-substituted metal contents, or even frustrated outcome. An efficient hydrothermal synthesis strategy for metallosilicate, in this case of Ta framework-substituted *BEA zeolite, via structural reconstruction was proposed to stride the gap. The Ta content in our developed Ta-Beta-Re-50 zeolite achieved up to 5.48 % (Si/Ta = 52), breaking through the limitation of Ta contents for conventional method (Si/Ta > 100). Additionally, this Ta-Beta-Re zeolite possessed nanosized crystals (20-40 nm) and short crystallization time (8 h), significantly improving space-time yields of practical zeolite production. Through spectroscopic study, it was confirmed that the existence of zeolite structural units intensively facilitated the formation of nucleation and crystal growth. This innovative Ta-Beta zeolite demonstrated high catalytic performances for oxidation desulfurization, far outperforming traditional fluoride-mediated Ta-Beta-F, which was ascribed to its excellent diffusion properties and incredible high isolated Ta contents. Additionally, the catalytic performance of Ta-Beta-Re could be regenerated after simple calcination and the deactivation may be caused by pore blocking of organics. This work provides a new method for rationally design and construction of metallosilicate materials with high activity for catalytic oxidation applications, which can bridge the conceptual and technical gap between periodic trends and zeolite material synthesis.

2.
Int Orthop ; 2020 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-32193610

RESUMO

PURPOSE: To investigate the effect of C3/4 disc degeneration on cervical spondylosis with dizziness (CSD) and to assess the curative effect of anterior cervical decompression and fusion (ACDF) in patients with CSD. METHOD: Four hundred nineteen patients who underwent ACDF for treatment of myelopathy or radiculopathy were divided into dizziness and non-dizziness group. The visual analog scale (VAS) score and Japanese Orthopaedic Association (JOA) score were used to determine the intensity of dizziness and neurological symptoms, respectively. Cervical disc degeneration was evaluated using Miyazaki's classification system. Some parameters were measured using cervical radiographs. The surgical effects on CSD were compared between surgery with and without C3/4 level. Multivariate logistic regression analysis was used to determine the risk factors for CSD. RESULTS: The pre-operative incidence of CSD was 33.9%. Women were more likely to develop dizziness than men (p < 0.05), CSD was significantly associated with C3/4 disc degeneration (69.7%, p < 0.001), and smokers were more subject to dizziness (p < 0.05). Regression analysis showed that female (OR = 1.611, p = 0.031), smoking (OR = 1.719, p = 0.032), Miyazaki grade of C3/4 ≥ IV (OR = 2.648, p < 0.001), and instability on C3/4 (OR = 1.672, p = 0.024) were risk factors for CSD. Treatment of CSD by ACDF involving C3/4 was more effective than not involving C3/4 (efficacy rate, 73.2% vs 51.7%, p < 0.05). CONCLUSION: The CSD is a common clinical manifestation in elderly patients, especially patients with cervical spondylosis at the C3/4 level. Female, smoking, instability on C3/4, and C3/4 Miyazaki grade ≥ IV could be considered significant risk factors for CSD. CSD is more likely to be alleviated by ACDF involving C3/4.

3.
BMC Med Inform Decis Mak ; 20(1): 53, 2020 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-32160884

RESUMO

BACKGROUND: Informatics tools to support the integration and subsequent interrogation of spatiotemporal data such as clinical data and environmental exposures data are lacking. Such tools are needed to support research in environmental health and any biomedical field that is challenged by the need for integrated spatiotemporal data to examine individual-level determinants of health and disease. RESULTS: We have developed an open-source software application-FHIR PIT (Health Level 7 Fast Healthcare Interoperability Resources Patient data Integration Tool)-to enable studies on the impact of individual-level environmental exposures on health and disease. FHIR PIT was motivated by the need to integrate patient data derived from our institution's clinical warehouse with a variety of public data sources on environmental exposures and then openly expose the data via ICEES (Integrated Clinical and Environmental Exposures Service). FHIR PIT consists of transformation steps or building blocks that can be chained together to form a transformation and integration workflow. Several transformation steps are generic and thus can be reused. As such, new types of data can be incorporated into the modular FHIR PIT pipeline by simply reusing generic steps or adding new ones. We validated FHIR PIT in the context of a driving use case designed to investigate the impact of airborne pollutant exposures on asthma. Specifically, we replicated published findings demonstrating racial disparities in the impact of airborne pollutants on asthma exacerbations. CONCLUSIONS: While FHIR PIT was developed to support our driving use case on asthma, the software can be used to integrate any type and number of spatiotemporal data sources at a level of granularity that enables individual-level study. We expect FHIR PIT to facilitate research in environmental health and numerous other biomedical disciplines.

4.
Sci Data ; 7(1): 90, 2020 03 12.
Artigo em Inglês | MEDLINE | ID: mdl-32165614

RESUMO

Turbot (Scophthalmus maximus) is a commercially important flatfish species in aquaculture. It has a drastic sexual dimorphism, with females growing faster than males. In the present study, we sequenced and de novo assembled female and male turbot genomes. The assembled female genome was 568 Mb (scaffold N50, 6.2 Mb, BUSCO 97.4%), and the male genome was 584 Mb (scaffold N50, 5.9 Mb, BUSCO 96.6%). Using two genetic maps, we anchored female scaffolds representing 535 Mb onto 22 chromosomes. Annotation of the female anchored genome identified 87.8 Mb transposon elements and 20,134 genes. We identified 17,936 gene families, of which 369 gene families were flatfish specific. Phylogenetic analysis showed that the turbot, Japanese flounder and Chinese tongue sole form a clade that diverged from other teleosts approximately 78 Mya. This report of female and male turbot draft genomes and annotated genes provides a new resource for identifying sex determination genes, elucidating the evolution of adaptive traits in flatfish and developing genetic techniques to increase the sustainability of turbot aquaculture.

5.
Int J Med Sci ; 17(6): 745-750, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32218696

RESUMO

Both the herpes zoster virus and suid herpesvirus type 1 (SuHV-1) belong to the Varicellovirus genus of the α-herpesviridae subfamily. They may cause opportunistic infections especially in patients with kidney diseases, varying from latent illness to overt lethality. Under these circumstances, impaired renal function is both the culprit for and victim of the infection. However, fulminant eruption of severe skin herpes zoster in lupus nephritis (LN) patients under prolonged immunosuppressive therapy is rare and even more rarely seen is the SuHV-1 encephalitis in human. Facing the evolution of these rare infections, we hence chose to review the clinical pathogenicity of these two viruses which were cognate in origin but distinct in virulence. As such, we began with the first of the two above viral diseases and proceeded with peculiar renal involvement, unique clinical symptoms and pertinent lethal risk. Of importance, LN was used to exemplify the reciprocally detrimental interactions between impaired renal function and suppressed immune response. Then in a manner similar to the gradient overlay, SuHV-1 encephalitis was discussed focusing on its neurotropic features, specific MRI findings and exclusive test of high throughput sequencing. Our report highlighted novel presentations of the Varicellovirus genus infection by providing a productive multidisciplinary communication with pointed disclosure of the renal involvement. It may therefore be of great medical relevance and educational value for clinicians, especially the unseasoned ones, to foresee and manage similar cases in susceptible patients.

6.
Sci Total Environ ; 720: 137431, 2020 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-32145615

RESUMO

Aircraft engine emissions during landing and take-off cycle are commonly estimated on the basis of the International Civil Aviation Organization (ICAO) promulgated calculation model and emission parameters; however, the ICAO certified parameters are generally not applicable for an individual airport. In this study, the operation times, fuel and emission parameters of 8 aircraft models during taxi phase at Shanghai Hongqiao International Airport (SHA) are analyzed with the Aircraft Communication Addressing and Reporting System (ACARS) data, and compared with corresponding values referenced by the ICAO. The results show perceptible discrepancies between the SHA-specific and ICAO certified values. The taxi-out times at SHA are considerably overestimated (up to 35.3%) by ICAO for all the analyzed aircraft models, whereas the taxi-in durations are highly close to the ICAO referenced value with a variation within -6.3% to 9.7%. In the majority of cases, the localized fuel flows and emission indices (EIs) are overvalued by ICAO, and the extent of overestimation can be as large as 21.6% and 28.3%, respectively. Variabilities in operation times, fuel and emission parameters are also characterized depending on aircraft types. Additionally, as a novel attempt, the effect of engine aging on EIs is explored and no significant correlation has been detected, indicating that other factors may affect the EIs dominantly over engine age. The resulting SHA-specific emission parameters are significant towards a precise emission quantification and modeling of impacts on air quality and health.

7.
Artigo em Inglês | MEDLINE | ID: mdl-32187779

RESUMO

Very recently, hydrovoltaic technology is emerging as a novel renewable energy harvesting method through carefully controlling the interaction between water and solid surface, which dramatically extends the capability to harvest water energy. However, the urgent issue restricting its device performance is poor carrier transport properties of the solid surface if large charged interface is considered simultaneously. Here, a hydrovoltaic device based on silicon nanowire arrays (SiNWs), which provide large charged surface/volume ratio and excellent carrier transport properties simultaneously, yields sustained electricity via carrier concentration gradient induced by evaporation-induced water flow inside nanochannels. And a device can yield direct current with a short-circuit current density of over 55 µA/cm 2 , which is three orders larger than previous reported analogous device (approximately 40 nA/cm 2 ). In addition, this device exhibits a constant output power density of over 6 µW/cm 2 and an open-circuit voltage of up to 400 mV, which enables it to easily drive a commercial light emitting diode. We also demonstrate the potential application of this electrokinetic phenomenon by using it to create a breathing sensor. Through changing ion concentration in water, length and surface charge of SiNWs, we propose the mechanism of harvesting energy through water flow in SiNWs. Our findings reveal that the generated energy should be mainly correlated with Debye screening effect and Coulomb interactions. Our finding may pave a way for developing innovative energy-harvesting devices from ubiquitous evaporation-driven internal water flow in nature with semiconductor material of silicon.

8.
Urol J ; 2020 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-32207138

RESUMO

PURPOSE: To describe our experience and analyze the outcomes of robot-assisted laparoscopic ureteral reimplantation (RALUR) and conventional laparoscopic ureteral reimplantation (LUR) in treating benign distal ureteral stricture (DUS). MATERIAL AND METHODS: Patients who underwent RALUR or LUR for DUS were retrospectively analyzed. All surgeries were performed by transperitoneal approach in a refluxing manner. Baseline characteristics, history of previous abdominal surgery, operative profile and follow-up data were collected and analyzed. RESULTS: Among 68 patients with DUS, 62 were diagnosed with unilateral DUS, including 28 patients underwent RALUR. The mean operative time of the RALUR group was 2.44 ± .45 hours, while the mean operative time of the LUR group was 3.09 ± .74 hours (P < .001). The average suturing time of LUR (39.59 ± 3.78 min) is about 2 times that of RALUR (20.04 ± 3.5 min) (P < .001). The success rate of the RALUR group and the LUR group were 89.3% and 82.4% respectively (P = .494). In multiple linear regression model, the modality of surgery was the only variable that influences operative time (Beta = -.964, P < .001), suturing time (Beta = -1.899, P < .001) and hemoglobin decline (Beta = -.611, P = .020). CONCLUSIONS: Basically, the postoperative outcomes are similar but robotic surgery offers a quicker surgery and anastomosis.

9.
Science ; 367(6483): 1255-1260, 2020 03 13.
Artigo em Inglês | MEDLINE | ID: mdl-32165587

RESUMO

T cells maintain a quiescent state prior to activation. As inappropriate T cell activation can cause disease, T cell quiescence must be preserved. Despite its importance, the mechanisms underlying the "quiescent state" remain elusive. Here, we identify BTG1 and BTG2 (BTG1/2) as factors responsible for T cell quiescence. BTG1/2-deficient T cells show an increased proliferation and spontaneous activation due to a global increase in messenger RNA (mRNA) abundance, which reduces the threshold to activation. BTG1/2 deficiency leads to an increase in polyadenylate tail length, resulting in a greater mRNA half-life. Thus, BTG1/2 promote the deadenylation and degradation of mRNA to secure T cell quiescence. Our study reveals a key mechanism underlying T cell quiescence and suggests that low mRNA abundance is a crucial feature for maintaining quiescence.

10.
Acta Cir Bras ; 35(1): e202000104, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32159588

RESUMO

PURPOSE: Glutamine, as an essential part of enteral nutrition and parenteral nutrition agent, has been widely recognized to be a kind of important intestinal mucosa protectant in clinical practice and experimental research. However, the mechanisms of its protective effects are still not fully understand. Consequently, this study aimed to explore the potential mechanism of glutamine on ischemia-reperfusion (I/R) injury induced endoplasmic reticulum (ER) stress in intestine. METHODS: An experimental model of intestinal I/R in rats was established by 1 hour occlusion of the superior mesenteric artery followed by 3 hours of reperfusion. Morphologic changes of intestinal mucosa, apoptosis of epithelial cells, and expression of intestinal Grp78, Gadd153, Caspase-12, ATF4, PERK phosphorylation (P-PERK) and elF2αphosphorylation(P-elF2α) were determined. RESULTS: After I/R, the apoptotic index of intestinal mucosa epithelial cells observably increased with notable necrosis of intestinal mucosa, and the expressions of Grp78, Gadd153, Caspase-12, ATF4, P-PERK and P-elF2αall were increased. However, treatment with glutamine could significantly relieve intestinal I/R injury and apoptosis index. Moreover, glutamine could clearly up-regulate the expression of Grp78, restrain P-PERK and P-elF2α, and reduce ATF4, Gadd153 and Caspase-12 expressions. CONCLUSION: Glutamine may be involved in alleviating ER stress induced intestinal mucosa cells apoptosis.


Assuntos
Apoptose/efeitos dos fármacos , Estresse do Retículo Endoplasmático/efeitos dos fármacos , Glutamina/farmacologia , Mucosa Intestinal/efeitos dos fármacos , Substâncias Protetoras/farmacologia , Traumatismo por Reperfusão/prevenção & controle , Fator 4 Ativador da Transcrição/efeitos dos fármacos , Animais , Caspase 12/efeitos dos fármacos , Proteínas de Choque Térmico/efeitos dos fármacos , Mucosa Intestinal/patologia , Mucosa Intestinal/ultraestrutura , Masculino , Artéria Mesentérica Superior/lesões , Modelos Animais , RNA Mensageiro/efeitos dos fármacos , Ratos Sprague-Dawley , Fator de Transcrição CHOP/efeitos dos fármacos , eIF-2 Quinase/efeitos dos fármacos
11.
Chemosphere ; 251: 126340, 2020 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-32135373

RESUMO

Bisphenol A (BPA) exposure during early life may increase risk of childhood obesity, however, prospective evidence of birth cohort is limited and inconclusive. We aimed to explore the associations of maternal and childhood BPA exposure with child adiposity measures, including body mass index, waist circumference and skinfold thickness and waist to height ratio of children at 7 years. 430 mother-child pairs were examined from a population-based prospective cohort in a rural area of East China. BPA concentrations of spot urine samples were quantified in mothers and their children aged 3 and 7 years. Maternal urinary BPA concentration was significantly positively associated with waist circumference in children aged 7 years (ß = 0.508 cm, 95% CI: 0.067, 0.950). These significant associations were not modified by child sex, but they were only observed among girls in sex-stratified analyses. Risk of central obesity related to prenatal BPA exposure was significantly higher in the second and the third tertile than those in the first tertile (odds ratio, OR = 2.510, 95% CI = 1.146, 5.499; OR = 2.584, 95% CI = 1.186, 5.631, respectively; p for trend = 0.022). The present findings suggested that prenatal exposure to BPA may enhance waist circumference of children and thereby increase risk of central obesity in school-age girls.

12.
J Sex Med ; 2020 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-32171629

RESUMO

BACKGROUND: Congenital hypogonadotropic hypogonadism (CHH) is a genetically heterogeneous disorder characterized by absent or incomplete puberty and infertility, and heterogeneous responses are often observed during treatment. AIM: To investigate the role of CHH-associated variants in patients with CHH with poor responses to human chorionic gonadotropin (hCG). METHODS: This retrospective study investigated 110 Chinese male patients with CHH undergoing genetic analysis and hCG treatment. CHH-associated rare sequence variants (RSVs) were identified by using a tailored next-generation sequencing panel and were interpreted in accordance with the American College of Medical Genetics and Genomics criteria. Clinical characteristics were recorded, and Kyoto Encyclopedia of Genes and Genomes analysis was conducted to assess pathways enriched in protein networks implicated in poor responses. OUTCOMES: The outcomes include testicular volume, serum hormonal profiles, parameters of semen analysis, pathogenicity classification, and pathway enrichment. RESULTS: Among the 110 patients, 94.55% achieved normal serum testosterone and 54.55% achieved seminal spermatozoa appearance (SSA). PLXNB1, ROBO3, LHB, NRP2, CHD7, and PLXNA1 RSVs were identified in patients who had an abnormal serum testosterone level during treatment. In spermatogenesis, the number of CHH-associated RSVs was not significantly strongly associated with delayed SSA. After pathogenicity classification, pathogenic/likely pathogenic (P/LP) RSVs were identified in 30% (33/110) of patients. Patients with P/LP RSVs showed delayed SSA compared with noncarriers, and P/LP PROKR2 RSVs showed the strongest association (48, 95% CI: 34.1-61.9 months, P = .043). Enriched pathways implicated in delayed SSA included neuroactive ligand-receptor interaction; Rap1, MAPK, PI3K-Akt signaling; and regulation of actin cytoskeleton. CLINICAL IMPLICATIONS: Male patients with CHH harboring P/LP PROKR2 RSVs should be aware of a high probability of poor responses to hCG; If these patients desire fertility, it might be better to recommend hCG/human menopausal gonadotropin, hCG/recombinant follicle-stimulating hormone, or pulsatile GnRH administration before treatments start or as early as possible. STRENGTHS & LIMITATIONS: Strengths are the standardized regimen and extensive follow-up (median time of 40 months). However, included patients in the study voluntarily chose hCG treatment because of the burden of drug cost and/or little fertility desire. Therefore, human menopausal gonadotropin or follicle-stimulating hormone was not added to this cohort. Our observed correlations should be further verified in patients with CHH undergoing other treatments. CONCLUSION: Among all P/LP RSVs, P/LP PROKR2 RSVs might correlate with poor responses in CHH under hCG treatment; our study supports the pathogenicity assessment of American College of Medical Genetics and Genomics criteria in genetic counseling, to improve management of patients with CHH. Chen Y, Sun T, Niu Y, et al. Correlations AmongGenotype and Outcome in Chinese Male Patients WithCongenital Hypogonadotropic Hypogonadism Under HCG Treatment. J Sex Med 2020;XX:XXX-XXX.

13.
Chem Commun (Camb) ; 2020 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-32211740

RESUMO

An innovative strategy based on dual structure-directing agent-facilitated crystallization was proposed to hydrothermally synthesize boron-free Zr-MWW and Sn-MWW metallosilicates that bear great structural diversity for potential pore engineering. The metallosilicates show distinctive features in Lewis acid-catalyzed reactions as efficient heterogeneous solid catalysts.

14.
Int J Nanomedicine ; 15: 1797-1807, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32214812

RESUMO

Purpose: Enhancing osteointegration of implants in osteoporosis patients is a necessity since implantations frequently fail in these patients. The aim of this work is to study how simvastatin-strontium-hydroxyapatite coated implants perform in rabbits with osteoporosis. Materials and Methods: Crystalline HA and Sr-HA oxide film were prepared through micro-arc oxidation. Surface characterization including morphology, roughness, element composition, phase composition, hydrophilicity were then evaluated. Simvastatin loaded on porous films through immersion, and the effects of coatings on osteointegration in osteoporotic rabbits were investigated. All samples were obtained after 4, 8 and 12 weeks of healing. Some of them were subjected to biomechanical tests and others were subjected to histological and histomorphometric analysis. Results: Coatings exhibited a microporous network structure with appropriate roughness and high hydrophilicity. Compared to control HA and machined surface implants, simvastatin-Sr-HA coated implants exhibited marked improvements in osteointegration, which is characterized by a quicker mineralization deposition rate, good bone formation mode (large amount of contact osteogenesis and a small amount of distance osteogenesis) and increased bone-to-implant contact and pull-out strength. Conclusion: These biological parameters demonstrate the excellent osteoconductivity of simvastatin-Sr-HA coatings in the osteoporotic state. Overall, this suggests that simvastatin-Sr-HA coatings would be applicable in poor-quality bones of patients experiencing osteoporosis.

15.
Bioresour Technol ; 307: 123208, 2020 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-32208342

RESUMO

Itaconic acid (IA) production by fermentation of undetoxified hydrolysate of bamboo residues by Aspergillus terreus was demonstrated. Monosaccharides were obtained by pretreatment and enzymatic hydrolysis of bamboo residues. A. terreus could not grow and synthesize IA in the hydrolysate. The buffer was confirmed to be an inhibitor, and was successfully replaced by deionized water as the suspension, to release equivalent sugar and eliminate the inhibition. Corn steep liquor significantly improved the adaptability of A. terreus to the hydrolysate at 2.0 g/L. The IA titer obtained (19.35 g/L IA) was the highest to be reported for IA production from lignocellulose without detoxification. Simultaneous saccharification and fermentation and fed-batch fermentation increased the titer to 22.43 g/L and 41.54 g/L, respectively. Meanwhile, economic assessment proved that bamboo residues were potential substrates for IA production with economic effectiveness.

16.
World Neurosurg ; 137: 140-145, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-32035201

RESUMO

BACKGROUND: Diffuse large B-cell lymphoma (DLBCL) is the most commonly diagnosed primary non-Hodgkin lymphoma of the spine and can induce spinal compression. Reports of lymphoma arising in bone adjacent to metallic prostheses are increasing. However, to our knowledge, DLBCL arising from a scar after lumbar fusion surgery has not been reported previously. CASE DESCRIPTION: A 63-year-old man complained of a 2-month history of severe pain in the back and both legs, radiating down to the ankle, similar to sciatica with a past history of L2-S1 decompression and fusion 7 years ago. Imaging revealed an irregular mass in the epidural space and around the internal fixation surgical site, which was initially diagnosed as an epidural infectious abscess. Most of the lesion was completely excised and a detailed immunohistopathologic analysis was performed revealing the diagnosis of a DLBCL. After surgery and chemotherapy, he was discharged without complications. Unfortunately, he died 2 years later because of brain metastasis. CONCLUSIONS: This case highlights the need to consider malignancy in the differential diagnosis and carefully examine surgical specimens in revision surgery. Further understanding of the role of metal implants in the development of lymphoma is required.

17.
Sensors (Basel) ; 20(4)2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-32075311

RESUMO

Vibration-based data-driven structural damage identification methods have gained large popularity because of their independence of high-fidelity models of target systems. However, the effectiveness of existing methods is constrained by critical shortcomings. For example, the measured vibration responses may contain insufficient damage-sensitive features and suffer from high instability under the interference of random excitations. Moreover, the capability of conventional intelligent algorithms in damage feature extraction and noise influence suppression is limited. To address the above issues, a novel damage identification framework was established in this study by integrating massive datasets constructed by structural transmissibility functions (TFs) and a deep learning strategy based on one-dimensional convolutional neural networks (1D CNNs). The effectiveness and efficiency of the TF-1D CNN framework were verified using an American Society of Civil Engineers (ASCE) structural health monitoring benchmark structure, from which dynamic responses were captured, subject to white noise random excitations and a number of different damage scenarios. The damage identification accuracy of the framework was examined and compared with others by using different dataset types and intelligent algorithms. Specifically, compared with time series (TS) and fast Fourier transform (FFT)-based frequency-domain signals, the TF signals exhibited more significant damage-sensitive features and stronger stability under excitation interference. The utilization of 1D CNN, on the other hand, exhibited some unique advantages over other machine learning algorithms (e.g., traditional artificial neural networks (ANNs)), particularly in aspects of computation efficiency, generalization ability, and noise immunity when treating massive, high-dimensional datasets. The developed TF-1D CNN damage identification framework was demonstrated to have practical value in future applications.

18.
Onco Targets Ther ; 13: 1593-1603, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32110056

RESUMO

Background: miR-26b-5p is reported to be involved in the progression of multiple cancers, but its function and mechanism in human papillary thyroid cancer (PTC) remain unknown. We aimed to uncover the function and mechanism of miR-26b-5p in PTC. Methods: We performed qRT-PCR to detect the differences in miR-26b-5p expression between normal tissue and PTC. In vitro, we established cell lines stably overexpressing miR-26b-5p and investigated the function and underlying mechanism of miR-26b-5p in PTC. Results: miR-26b-5p was downregulated in PTC compared with normal tissue. miR-26b-5p was correlated with the clinical stage. miR-26b-5p inhibited the proliferation, invasion and migration of PTC cell lines. We next detected EMT and proliferation markers. miR-26b-5p was shown to exert its function in a ß-catenin-dependent manner. Conclusion: Taken together, our results showed that miR-26b-5p inhibits proliferation, migration, invasion and EMT by degrading ß-catenin.

19.
Infect Drug Resist ; 13: 597-605, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32110070

RESUMO

Purpose: To investigate the occurrence and genetic characteristics of the bla IMP-26-positive plasmid from a multidrug-resistant clinical isolate, Enterobacter hormaechei L51. Methods: Species identification was determined by MALDI-TOF MS and Sanger sequencing. Antimicrobial susceptibility testing was performed by the agar dilution and broth microdilution. Whole-genome sequencing was conducted using Illumina HiSeq 4000-PE150 and PacBio Sequel platforms, and the genome was annotated by the RAST annotation server. The ANI analysis of genomes was performed using OAT. Phylogenetic reconstruction and analyses were performed using the Harvest suite based on the core-genome SNPs of 61 publicly available E. hormaechei genomes. Results: The E. hormaechei L51 genome consists of a 5,018,729 bp circular chromosome and a 343,918 bp conjugative IncHI2/2A plasmid pEHZJ1 encoding bla IMP-26 which surrounding genetic context was intI1-bla IMP-26-ltrA-qacE∆1-sul1. A new sequence type (ST1103) was assigned for the isolate L51 which was resistant to cephalosporins, carbapenems, but sensitive to piperacillin-tazobactam, amikacin, tigecycline, trimethoprim-sulfamethoxazole and colistin. Phylogenetic analysis demonstrated that E. hormaechei L51 belonged to the same subspecies as the reference strain E. hormaechei SCEH020042, however 18,248 divergent SNP were identified. Resistance genes in pEHZJ1 including aac(3)-IIc, aac(6') -IIc, bla SHV-178, bla DHA-1, bla TEM-1, bla IMP-26, ereA2, catII, fosA5, qnrB4, tet(D), sul1 and dfrA19. Conclusion: In our study, we identified a conjugative IncHI2/2A plasmid carrying bla IMP-26 and bla SHV-178 in E. hormaechei ST1103, a novel multidrug-resistant strain isolated from China, and describe the underlying resistance mechanisms of the strain and detailed genetic context of mega plasmid pEHZJ1.

20.
Environ Pollut ; 261: 114115, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-32045794

RESUMO

The air traffic growth at Shanghai Pudong International Airport (PVG) has attracted much concern over the potential impacts on local air quality and human health; however, the emission contributions due to aircraft activities, impact on air quality and health effects remain unclear. In this study, the ground operational data derived from the Aircraft Communication Addressing and Reporting System (ACARS) dataset are newly utilized to obtain the PVG-specific emission parameters of 10 distinct aircraft-engine combinations during the taxi-in and taxi-out phases of the landing and take-off (LTO) cycle. The resulting emission parameters, together with PVG-specific operational conditions, are applied to quantify the annual emissions in 2017 for main engines and auxiliary power units (APUs) at PVG, emission variations caused by mixing layer height, sensitivity of black carbon (BC) emissions to the estimation method and sensitivity of PM2.5 emissions to the fuel sulfur content (FSC). The results show noticeable discrepancies between the corrected fuel flows and NOx emission indices (EIs) and those certified by the International Civil Aviation Organization (ICAO). The annual emissions of hydrocarbons (HC), CO, NOx, NO, NO2, HONO, HNO3, NOy, SO2, SO42-, BC, organic carbon (OC) and PM2.5 with corrected emission parameters are 3.82 × 105 kg, 4.35 × 106 kg, 5.36 × 106 kg, 4.40 × 106 kg, 9.58 × 105 kg, 1.03 × 105 kg, 3.83 × 103 kg, 5.47 × 106 kg, 3.56 × 105 kg, 1.31 × 104 kg, 5.43 × 104 kg, 4.73 × 103 kg and 7.22 × 104 kg, respectively, while the application of the maximum height of the mixing layer contributes to emission increases as high as 16.9% (NOx). An alternative estimation of BC emissions leads to an increase of 50% compared with first-order approximation 3 (FOA3), while a reduction in PM2.5 emissions can be expected by minimizing the FSC.

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