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1.
BMC Cancer ; 20(1): 132, 2020 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-32070301

RESUMO

BACKGROUND: Microvascular invasion (MVI) is an independent risk factor for poor prognosis in hepatocellular carcinoma (HCC). However, there is still a lack of preoperative markers to predict MVI in HCC. This study intends to explore the potential application value of the gamma-glutamyl transpeptidase (GGT) to lymphocyte count ratio (GLR) in predicting MVI in HCC and provide guidance for clinical diagnosis and treatment. METHODS: From March 2010 to December 2015, 230 HCC patients who underwent surgical treatment in the Affiliated Hospital of Guilin Medical University were selected. Clinicopathological parameters between the MVI group (n = 115) and the non-MVI group (n = 115) were comparatively analyzed. The GLR was used as the potential risk factor for HCC with MVI, and its optimal cut-off value was estimated by using the receiver operating characteristic (ROC) curve. The Kaplan-Meier method was used to analyze the survival of HCC patients, and univariate and multivariate Cox regression analyses were used to establish independent predictors affecting postoperative HCC patients. RESULTS: The GLR levels in the MVI group and non-MVI group were 84.83 ± 61.84 and 38.42 ± 33.52 (p <  0.001), respectively. According to ROC curve analysis, the optimal cut-off value of GLR was 56.0, and the area under the ROC curve (AUC) was 0.781 (95% CI, 0.719-0.833) for the risk prediction of MVI in HCC patients. Multivariate analysis showed that tumor size > 5 cm, HCC combined with MVI and GLR >  56.0 were independent risk factors for poor prognosis in HCC patients. In addition, compared with the non-MVI group, patients in the MVI group had shorter progression-free survival (PFS) and overall survival (OS). CONCLUSION: GLR could be a predictive biomarker of HCC after operation and a potential predictor of HCC combined with MVI.

2.
Cell Prolif ; 53(2): e12743, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31943455

RESUMO

OBJECTIVES: Alveolar bone osteoporosis has attracted more and more attention because of its profound impact on stomatognathic function and treatment, but current treatments have not been targeted to alveolar bone and might even cause severe side effects. Thus, identifying the effects of anti-osteoporosis agents on alveolar bone is essential. Icariin ameliorates metabolic dysfunction of long bones, but its effects on alveolar bone remain unclarified. MATERIALS AND METHODS: BMSCs were isolated from rat mandibles (mBMSCs). The osteogenic potential of mBMSCs and the signalling pathway involved under icariin treatment were measured by ALP and alizarin red staining, reverse transcription-polymerase chain reaction (RT-PCR), Western blotting and immunofluorescence. Dual-luciferase assay, chromatin immunoprecipitation (ChIP) and co-immunoprecipitation were used to investigate the molecular mechanism. Ovariectomized and sham-operated rats treated with or without icariin were analysed by micro-CT, TRAP staining and calcein double labelling. RESULTS: We found that icariin promoted osteoblast differentiation of mBMSCs. Furthermore, STAT3 was critical for icariin-promoted osteoblast differentiation, as indicated by increased phosphorylation levels in icariin-treated mBMSCs, while preventing STAT3 activation blocked icariin-induced osteoblast differentiation. Mechanistically, icariin-promoted transcription of the downstream osteogenic gene osteocalcin (Ocn) through STAT3 and STAT3 bound to the promoter of Ocn. Notably, icariin prevented the alveolar bone osteoporosis induced by oestrogen deficiency through promoting bone formation. CONCLUSIONS: For the first time, our work provides evidence supporting the potential application of icariin in promoting osteogenesis and treating alveolar bone osteoporosis.


Assuntos
Perda do Osso Alveolar/tratamento farmacológico , Estrogênios/metabolismo , Flavonoides/farmacologia , Osteogênese/efeitos dos fármacos , Fator de Transcrição STAT3/metabolismo , Perda do Osso Alveolar/metabolismo , Animais , Diferenciação Celular/efeitos dos fármacos , Células Cultivadas , Células-Tronco Mesenquimais/efeitos dos fármacos , Células-Tronco Mesenquimais/metabolismo , Osteoblastos/efeitos dos fármacos , Osteoblastos/metabolismo , Osteocalcina/efeitos dos fármacos , Osteocalcina/metabolismo , Osteoporose/tratamento farmacológico , Osteoporose/metabolismo , Fosforilação/efeitos dos fármacos , Ratos , Transdução de Sinais/efeitos dos fármacos , Transcrição Genética/efeitos dos fármacos
3.
J Biomed Sci ; 27(1): 19, 2020 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-31907023

RESUMO

BACKGROUND: The FDA approved drug granulocyte-colony stimulating factor (G-CSF) displays anti-apoptotic and immunomodulatory properties with neurogenesis and angiogenic functions. It is known to demonstrate neuroprotective mechanisms against ischemic global stroke. Autophagy is a method for the degradation of intracellular components and in particular, unrestrained autophagy may lead to uncontrolled digestion of affected neurons as well as neuronal death in cerebral ischemia. Mitochondrial dynamics is vital for the regulation of cell survival and death after cerebral ischemia and an early upstream event in neuronal death is mitochondrial fission. We examined the pro-survival mechanisms of G-CSF against apoptosis resulting from autophagy, mitochondrial stress and endoplasmic reticulum (ER) stress. METHODS: Male Swiss Webster mice (20 weeks of age) were subjected to bilateral common carotid artery occlusion (BCAO) for 30 min. After occlusion, mice were injected with G-CSF (50 µg/kg) subcutaneously for 4 days. Behavioral analysis was carried out using the corner test and locomotor activity test before animals were sacrificed on day 4 or day 7. Key proteins in ER stress, autophagy and mitochondrial stress induced apoptosis were analyzed by immunoblotting. RESULTS: G-CSF improved neurological deficits and improved behavioral performance on corner and locomotor test. G-CSF binds to G-CSF receptors and its activation leads to upregulation of Akt phosphorylation (P-Akt) which in turn decreases levels of the ER stress sensor, GRP 78 and expression of proteins involved in ER stress apoptosis pathway; ATF6, ATF4, eIF2α, XBP1, Caspase 12 and CHOP. G-CSF treatment significantly decreased Beclin-1, an autophagy marker, and decreased mitochondrial stress biomarkers DRP1 and P53. G-CSF also up-regulated the mitochondrial fusion protein, OPA1 and anti-apoptotic protein Bcl-2 while down-regulating the pro-apoptotic proteins Bax, Bak and PUMA. CONCLUSIONS: G-CSF is an endogenous ligand in the CNS that has a dual activity that is beneficial both in reducing acute neuronal degeneration and adding to long-term plasticity after cerebral ischemia. G-CSF treatment exerts neuroprotective effects on damaged neurons through the suppression of the ER stress and mitochondrial stress and maintains cellular homeostasis by decreasing pro-apoptotic proteins and increasing of anti-apoptotic proteins.

4.
J Oral Maxillofac Surg ; 78(3): 386-393, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31862339

RESUMO

PURPOSE: Descending necrotizing mediastinitis (DNM) has been the most common life-threatening complication of multispace infection (MSI) in the maxillofacial region owing to the lack of a timely diagnosis and treatment. We assessed the clinical characteristics and diagnosis of odontogenic MSI and evaluated the risk factors for DNM caused by MSI. PATIENTS AND METHODS: We performed a retrospective cohort study of inpatients with MSI in the maxillofacial region from January 2012 to October 2016. The patients were classified into a non-DNM group and a secondary DNM group. The information collected included gender, age, systemic comorbidities, source of maxillofacial infection, computed tomography imaging data, and laboratory test results. Univariate analysis (t test and χ2 test, or the Fisher exact test) and logistic regression analysis were applied. RESULTS: A total of 296 patients were included. The mortality was 6.3%. On univariate analysis, the following factors were statistically significant: gender (P = .001); age (P = .003); source of infection (P = .004); number of affected spaces (P < .001); involvement of the parotid space (P < .001), submandibular space (P < .001), subgingival space (P < .001), pterygomandibular space (P < .001), parapharyngeal space (P < .001), and retropharyngeal space (P < .001); and percentage of neutrophils (P < .001). On multivariate analysis, the parapharyngeal space (P = .008), source of infection (P = .037), and number of affected spaces (P < .001) were statistically significant. CONCLUSIONS: Glandular infection, parapharyngeal space involvement, and the presence of multiple affected spaces were risk factors for DNM. Clinicians should vigilantly watch for these factors during clinical treatment and effective measures taken to prevent the occurrence of DNM as soon as possible.

5.
J Biol Chem ; 294(42): 15395-15407, 2019 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-31462535

RESUMO

The transcription factor signal transducer and activator of transcription 3 (STAT3) plays a central role in cell survival and function. STAT3 has been demonstrated to participate in the maintenance of bone homeostasis in osteoblasts, but its role in osteoclasts in vivo remains poorly defined. Here, we generated a conditional knockout mouse model in which Stat3 was deleted in osteoclasts using a cathepsin K-Cre (Ctsk-Cre) driver. We observed that osteoclast-specific Stat3 deficiency caused increased bone mass in mice, which we attributed to impaired bone catabolism by osteoclasts. Stat3-deficient bone marrow macrophages (BMMs) showed decreased expression of nuclear factor of activated T cells, cytoplasm 1 (NFATc1), and reduced osteoclast differentiation determined by decreases in osteoclast number, tartrate-resistant acid phosphatase activity, and expression of osteoclast marker genes. Enforced expression of NFATc1 in Stat3-deficient BMMs rescued the impaired osteoclast differentiation. Mechanistically, we revealed that STAT3 could drive the transcription of NFATc1 by binding to its promoter. Furthermore, preventing STAT3 activation by using an inhibitor of upstream phosphorylases, AG490, also impaired osteoclast differentiation and formation in a similar way as gene deletion of Stat3 In summary, our data provide the first evidence that STAT3 is significant in osteoclast differentiation and bone homeostasis in vivo, and it may be identified as a potential pharmacological target for the treatment of bone metabolic diseases through regulation of osteoclast activity.

6.
Neuroimage ; 174: 208-218, 2018 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-29567502

RESUMO

Two identical visual disks moving towards one another on a two-dimensional display can be perceived as either "streaming through" or "bouncing off" each other after their coincidence/overlapping. A brief sound presented at the moment of the coincidence of the disks could strikingly bias the percept towards bouncing, which was termed the audiovisual bounce-inducing effect (ABE). Although the ABE has been studied intensively since its discovery, the debate about its origin is still unresolved so far. The present study used event-related potential (ERP) recordings to investigate whether or not early neural activities associated with cross-modal interactions play a role on the ABE. The results showed that the fronto-central P2 component ∼200 ms before the coincidence of the disks was predictive of subsequent streaming or bouncing percept in the unimodal visual display but not in auditory-visual display. More importantly, the cross-modal interactions revealed by the fronto-central positivity PD170 (125-175 ms after sound onset), as well as the occipital positivity PD190 (180-200 ms), were substantially enhanced on bouncing trials compared to streaming trials in the auditory-visual display. These findings provide direct electrophysiological evidence that early cross-modal interactions contribute to the origin of ABE phenomenon at the perceptual stage of processing.


Assuntos
Percepção Auditiva/fisiologia , Encéfalo/fisiologia , Percepção de Movimento/fisiologia , Estimulação Acústica , Adulto , Eletroencefalografia , Potenciais Evocados Auditivos , Potenciais Evocados Visuais , Feminino , Humanos , Masculino , Estimulação Luminosa , Adulto Jovem
7.
Mol Med Rep ; 16(6): 8673-8684, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28990076

RESUMO

Acute gastric lesions induced by stress are frequent occurrences in medical establishments. The gastric dramatic downrelated gene (GDDR) is a secreted protein, which is abundantly expressed in normal gastric epithelia and is significantly decreased in gastric cancer. In our previous study, it was found that GDDR aggravated stress­induced acute gastric lesions. However, the role of GDDR in acute gastric lesions remains to be fully elucidated. In the present study, RNA sequencing was performed in order to examine the gene expression profile regulated by GDDR in acute gastric lesions. The dataset comprised four stomach samples from wild-type (WT) mice and four stomach samples from GDDR­knockout mice. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to analyze the differentially-expressed genes (DEGs). Weighted correlation network analysis was used to identify clusters of highly correlated genes. Cytoscape was used to construct a protein­protein interaction network (PPI) of the DEGs. Based on the GO analysis, the upregulated DEGs were distinctly enriched in muscle contraction and response to wounding; and the downregulated DEGs were significantly enriched in the regulation of nitrogen compound metabolic process and regulation of RNA metabolic process. The results of the KEGG pathway analysis showed that the upregulated DEGs were enriched in ECM­receptor interaction and the signaling pathway of cGMP­PKG, and the downregulated DEGs were enriched in the renin­angiotensin system and glycerolipid metabolism. The co­expression network revealed a group of genes, which were associated with increased wound healing in the WT mice. Significant pathways were identified through the PPI network, including negative regulation of the signaling pathway of glucocorticoid receptor, regulation of cellular stress response, and regulation of hormone secretion. In conclusion, the present study improves current understanding of the molecular mechanism underlying acute gastric lesions and may assist in the treatment of gastric lesions.


Assuntos
Proteínas de Transporte/genética , Perfilação da Expressão Gênica , Transcriptoma , Animais , Biologia Computacional/métodos , Ontologia Genética , Redes Reguladoras de Genes , Sequenciamento de Nucleotídeos em Larga Escala , Masculino , Camundongos , Fenótipo , Mapeamento de Interação de Proteínas , Mapas de Interação de Proteínas , Reprodutibilidade dos Testes , Análise de Sequência de RNA
8.
J Exerc Sci Fit ; 14(2): 60-66, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29541120

RESUMO

Background/Objective: This work describes a new approach for gait analysis and balance measurement. It uses an inertial measurement unit (IMU) that can either be embedded inside a dynamically unstable platform for balance measurement or mounted on the lower back of a human participant for gait analysis. Methods and Results: The acceleration data along three Cartesian coordinates is analyzed by the gait-force model to extract bio-mechanics information in both the dynamic state as in the gait analyzer and the steady state as in the balance scale. For the gait analyzer, the simple, noninvasive and versatile approach makes it appealing to a broad range of applications in clinical diagnosis, rehabilitation monitoring, athletic training, sport-apparel design, and many other areas. For the balance scale, it provides a portable platform to measure the postural deviation and the balance index under visual or vestibular sensory input conditions. Despite its simple construction and operation, excellent agreement has been demonstrated between its performance and the high-cost commercial balance unit over a wide dynamic range. Conclusion: The portable balance scale is an ideal tool for routine monitoring of balance index, fall-risk assessment, and other balance-related health issues for both clinical and household use.

9.
Med Oncol ; 30(3): 658, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23884578

RESUMO

Single-nucleotide polymorphisms (SNPs) of adiponectin (ADIPOQ), adiponectin receptor 1 (ADIPOR1) and ADIPOR2 genes contribute to the risk and progression of cancers. Here, we investigated the associations between variants of these three genes and the risk of gastric cancer. We genotyped six ADIPOQ SNPs, nine ADIPOR1 SNPs and six ADIPOR2 SNPs using the Sequenom technique in a hospital-based case-control study of patients with gastric cancer and cancer-free controls in the Chinese Han population. We found associations of certain variants with location of gastric cancer. Rs16861205 with the minor allele A in ADIPOQ, rs10773989 with the minor allele C and rs1044471 with the minor allele T in ADIPOR2 presented significant associations with a decreased risk of cardia cancer (P = 0.024, OR 0.605, 95 % CI 0.390-0.938; P = 0.015, OR 0.699, 95 % CI 0.522-0.935; and P = 0.022, OR = 0.703, 95 % CI 0.519-0.951, respectively). ADIPOQ rs16861205 with minor allele A displayed an association with an increased risk of body cancer (P = 0.010, OR 1.821, 95 % CI 1.148-2.890). Further stratified analysis of the patients indicated that there were significant correlations for rs1342387A/G (P = 0.027) and rs16861205A/G (P = 0.000) with tumor location; rs16850799A/G (P = 0.004) and rs2058033C/A (P = 0.003) with invasion depth; rs16850799A/G (P = 0.019) with the tumor-node-metastasis stage; rs16850799A/G (P = 0.016), rs1501299A/C (P = 0.005) and rs1063538C/T (P = 0.017) with alcohol consumption; rs11612414A/G (P = 0.040) and rs12733285T/C (P = 0.005) with salted food; rs1063538C/T (P = 0.043) with family history of gastric cancer; and rs11612414A/G (P = 0.029) with gender. Adiponectin expression significantly correlated with gender (P = 0.014), alcohol consumption (P = 0.037), family history (P = 0.019) and invasion depth of primary tumor (P = 0.024). Our data suggested that variants of ADIPOQ may be genetic markers conferring susceptibility to gastric cancer subtypes. These findings need to be validated in a larger panel of samples from distinct populations.


Assuntos
Adiponectina/genética , Predisposição Genética para Doença/genética , Polimorfismo de Nucleotídeo Único/genética , Neoplasias Gástricas/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Alelos , Grupo com Ancestrais do Continente Asiático/genética , Biomarcadores Tumorais/genética , Estudos de Casos e Controles , Feminino , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Receptores de Adiponectina/genética , Risco , Neoplasias Gástricas/etiologia , Adulto Jovem
10.
Gene ; 515(1): 200-4, 2013 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-23246699

RESUMO

Human DOC-2/DAB2 interactive protein (hDAB2IP) gene is a novel member of the Ras GTPase-activating family and has been demonstrated to be a tumor-suppressor gene that inhibits cell survival and proliferation and induces cell apoptosis. It was reported that the expression level of hDAB2IP in gastric cancer tissue was highly correlated with tumor progression, however, whether hDAB2IP genetic variants are associated with the risk of gastric cancer remains yet unknown. In this case-control study, we conducted a genetic analysis for hDAB2IP variants in 311 patients with gastric cancer and 425 controls from the Chinese Han population. We found that the SNP rs2243421 of hDAB2IP gene with the minor allele C significantly revealed strong association with decreased gastric cancer susceptibility (P=0.007, adjusted odds ratio [OR]=0.734, 95%CI=0.586-0.919). Haplotypes rs2243421 and rs10985332 (HaploType: CC, P=0.012, aOR=0.760) and haplotypes rs2243421 and rs555996 (HaploType: CG, P=0.034, aOR=0.788) represented the decreased risk of gastric cancer, respectively. On the contrary, rs2243421 and rs555996 showed an elevated susceptibility (HaploType: TG, P=0.010, aOR=1.320). Our results for the first time provided new insight into susceptibility factors of hDAB2IP gene variants in carcinogenesis of gastric cancer.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Proteínas de Ligação ao Cálcio/genética , Predisposição Genética para Doença , Proteínas do Tecido Nervoso/genética , Polimorfismo de Nucleotídeo Único , Neoplasias Gástricas/genética , Proteínas Ativadoras de ras GTPase/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Alelos , China , Feminino , Frequência do Gene , Genótipo , Haplótipos , Humanos , Desequilíbrio de Ligação , Masculino , Pessoa de Meia-Idade , Neoplasias Gástricas/diagnóstico , Adulto Jovem
11.
Intern Med ; 51(6): 537-43, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22449659

RESUMO

OBJECTIVE: The objective of the present multi-ethnic study was to explore whether an association exists between brachial-ankle pulse wave velocity (baPWV) and cardiovascular risk factors in China. METHODS: Two hundred sixty-four adult subjects (152 subjects were of Han nationality, 70 subjects were from Uygur, 19 subjects were from Kazakh, 15 subjects were from Hui, and 8 subjects were of other nationalities.) aged 30-82 years were enrolled in the present study. The subjects' anthropometric, baPWV and laboratory measurements were recorded. RESULTS: Age (p=0.000), systolic blood pressure (p=0.000), diastolic blood pressure (p=0.002), fasting blood glucose (p=0.000), and hemoglobin (p=0.019) differed significantly among the subjects in the baPWV quartile. Spearman correlation analyses indicated that baPWV was significantly and positively associated with age (r=0.584, p=0.000), systolic blood pressure (r=0.396, p=0.000), diastolic blood pressure (r=0.217, p=0.000), and fasting blood glucose (r=0.231, p=0.000). baPWV was significantly and negatively associated with erythrocyte number (r=-0.181, p=0.003) and hemoglobin (r=-0.192, p=0.002) levels. Multiple regression analyses suggested that age (ß=0.573, p=0.000), systolic blood pressure (ß=0.181, p=0.003), triglycerides (ß=0.160, p=0.008), and cholesterol (ß=0.132, p=0.020) were independently associated with baPWV. CONCLUSION: Age, SBP, triglycerides, and cholesterol levels are independently and positively associated with baPWV.


Assuntos
Índice Tornozelo-Braço , Doenças Cardiovasculares/etnologia , Grupos Étnicos/estatística & dados numéricos , Adulto , Idoso , Idoso de 80 Anos ou mais , Consumo de Bebidas Alcoólicas/epidemiologia , Contagem de Células Sanguíneas , Glicemia/análise , Pressão Sanguínea , Índice de Massa Corporal , Doenças Cardiovasculares/epidemiologia , China/epidemiologia , Colesterol/sangue , Feminino , Hemoglobinas/análise , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Fatores de Risco , Fumar/epidemiologia , Triglicerídeos/sangue , Resistência Vascular
12.
Zhongguo Zhong Yao Za Zhi ; 32(6): 481-3, 2007 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-17552149

RESUMO

OBJECTIVE: To study and improve the tissue culture technology of Panax notoginseng. METHOD: Using the callus of leaf blade and leafstalk of P. notogingseng as explants, MS + 2, 4-D 1.5 mg x L(-1) as basal medium, the formation of asexual embryos was induced by added LFS, BA, KT or ZT 0.5 mg x L(-1), and cultured in dark. It cultured then in 2000 lx of illumination for 10-12 h x d(-1) to induce the asexual embryos germinating and developing to be the regenerated-plantlet. RESULT AND CONCLUSION: Only the medium added with LFS could induce the formation of asexual embryos, and made it developed to be regenerated-plantlet. The inducing ratio of asexual embryos reached about 85%, and 30% of asexual embryos could grow and develop as robust regenerated-plantlets.


Assuntos
Panax notoginseng/fisiologia , Folhas de Planta/fisiologia , Plantas Medicinais/fisiologia , Regeneração/fisiologia , Meios de Cultura/farmacologia , Panax notoginseng/embriologia , Reguladores de Crescimento de Planta/farmacologia , Folhas de Planta/embriologia , Plantas Medicinais/embriologia , Regeneração/efeitos dos fármacos , Técnicas de Cultura de Tecidos
13.
Zhong Yao Cai ; 27(1): 1-3, 2004 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-15179778

RESUMO

In order to explore quick, efficient inducement and proliferation of Panax notogingseng callus, the stem sections were taken as explants in the following 5 groups of medium: 1. MS + 2,4-D 2 mg/L (CK), 2. MS + 2,4-D 2 mg/L + N6-BA 2 mg/L, 3. MS + 2,4-D 2 mg/L + KT 2 mg/L, 4. MS + 2,4-D 2 mg/L + ZT 2 mg/L, 5. MS + 2,4-D 2 mg/L + LFS 2 mg/L. The results showed: 1. LFS (Lingfasu, a new kind of CTK) was able to promote the callus formation earlier 1-2 week than CK and to raise the rate of induced callus as high as 81%, higher 30% than that on other 4 mediums; 2. On medium 5, the fresh weight of callus increased 360.2% after being cultured 40 d, on other 4 mediums increased only 13.4%-21.8%. At the same time, 1 g callus cultured 40 d could obtain dry material 81.5 mg on medium 5, but on other 4 mediums only 21.5-25.9 mg; 3. The callus cultured on medium 5. continual generation to generation could live as long as more than 3 years, on other 4 mediums the callus hardened and aged quickly.


Assuntos
Adenosina/farmacologia , Panax/crescimento & desenvolvimento , Reguladores de Crescimento de Planta/farmacologia , Plantas Medicinais/crescimento & desenvolvimento , Adenosina/análogos & derivados , Meios de Cultura , Citocininas/farmacologia , Caules de Planta/crescimento & desenvolvimento , Técnicas de Cultura de Tecidos
14.
Zhong Yao Cai ; 27(8): 552-3, 2004 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-15658812

RESUMO

The qualitative analysis of the effective composition, matrine and oxymatrine in tissue-cultured and wild Sophora tonkinsis was done through thin-layer chromatography. The results showed that the tissue-cultured plants were almost as same as the wild plants in the ability to bio-synthesize matrine and oxymatrine. So, the tissue-cultured plants could be used as planting materials for artificial cultivation.


Assuntos
Alcaloides/análise , Plantas Medicinais/química , Sophora/química , Alcaloides/biossíntese , Alcaloides/isolamento & purificação , Cromatografia em Camada Delgada , Conservação dos Recursos Naturais , Plantas Medicinais/crescimento & desenvolvimento , Quinolizinas , Sophora/crescimento & desenvolvimento , Técnicas de Cultura de Tecidos
15.
Zhong Yao Cai ; 27(10): 711-2, 2004 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-15850351

RESUMO

On the medium MS added the right amount of 2,4-D and LFS (Angustmycin) and cultured under dark condition, the callus from stem segments of Panax notoginseng could induce a lot of embryoids. In 2-3 months, the ratio of embryoid formation reached about 90%. Then transplanted on MS + 2,4-D 1.5 mg/L + LFS 2 mg/L and cultured under light 20001x, near 30% embryoids could grow and develop as robust plantlets.


Assuntos
Panax/crescimento & desenvolvimento , Reguladores de Crescimento de Planta/farmacologia , Plantas Medicinais/crescimento & desenvolvimento , Adenosina/análogos & derivados , Adenosina/farmacologia , Meios de Cultura , Brotos de Planta/crescimento & desenvolvimento , Caules de Planta/crescimento & desenvolvimento , Técnicas de Cultura de Tecidos
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