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1.
Theriogenology ; 149: 38-45, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32234649

RESUMO

The presence of serum in embryo culture medium has been implicated for increased embryo's sensitivity to cryopreservation, compromised viability, abnormal embryo and fetal development. Hence, designing a serum free culture system is indispensable. The present study aims to compare the efficiency of the serum and granulosa cells monolayer free commercial culture system (SFCS) with the conventional serum supplemented co-culture system (SSCS) and optimized culture system (OCS). Generally, SFCS is designed explicitly for bovine oocyte maturation and embryo culture (SF-IVM and SF-IVC), and SSCS (based on M199, SS-IVM, and SS-IVC) is utilized for buffalo in vitro embryo production. However, OCS is a newly designed culture system in which oocyte maturation is performed in serum supplemented maturation medium, and the subsequent embryos are co-cultured with granulosa cells in serum free culture medium. To evaluate the effect of serum on buffalo embryo production, buffalo oocytes, and their subsequent embryos were cultured in SSCS, SFCS, and OCS, simultaneously. The percentage of cleaved embryos cultured in SSCS and OCS was approximately 4% higher as compared to SFCS. However, OCS significantly showed the maximum proportion of embryos that developed to the blastocyst stage (7d) and hatched (6d) as compared to the SFCS and SSCS. Additionally, OCS promoted the expression of developmentally important genes (BCL2-L1 and VEGF-A), cell number, and cryo-survival ability of blastocysts in comparison with SSCS. Taken together, OCS is more suitable for the oocyte maturation and culture of buffalo embryos. However, to design the serum free culture system, it is recommended to find suitable serum alternatives for in vitro oocyte maturation.

2.
Int J Mol Sci ; 21(6)2020 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-32183390

RESUMO

The molecular mechanism regulating embryo development under reduced oxygen tension remains elusive. This study aimed to identify the molecular mechanism impacting embryo development under low oxygen conditions. Buffalo embryos were cultured under 5% or 20% oxygen and were evaluated according to their morphological parameters related to embryo development. The protein profiles of these embryos were compared using iTRAQ-based quantitative proteomics. Physiological O2 (5%) significantly promoted blastocyst yield, hatching rate, embryo quality and cell count as compared to atmospheric O2 (20%). The embryos in the 5% O2 group had an improved hatching rate of cryopreserved blastocysts post-warming (p < 0.05). Comparative proteome profiles of hatched blastocysts cultured under 5% vs. 20% O2 levels identified 43 differentially expressed proteins (DEPs). Functional analysis indicated that DEPs were mainly associated with glycolysis, fatty acid degradation, inositol phosphate metabolism and terpenoid backbone synthesis. Our results suggest that embryos under physiological oxygen had greater developmental potential due to the pronounced Warburg Effect (aerobic glycolysis). Moreover, our proteomic data suggested that higher lipid degradation, an elevated cholesterol level and a higher unsaturated to saturated fatty acid ratio might be involved in the better cryo-survival ability reported in embryos cultured under low oxygen. These data provide new information on the early embryo protein repertoire and general molecular mechanisms of embryo development under varying oxygen levels.

3.
ACS Appl Mater Interfaces ; 12(4): 4722-4731, 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-31894961

RESUMO

Metal oxides with a polar surface interact strongly with polar NO2 molecules, thus facilitating sensitive detection of NO2. In this work, the composites comprising graphene and cubic CeO2 nanoparticles with the {100} polar surface are prepared by a hydrothermal technique, and they exhibit fast response, excellent selectivity, stable recovery, and sensitive detection with a low detection limitation of 1 ppm for NO2 at room temperature. According to the first-principle calculations, the adsorption energy of NO2 on the CeO2{100} polar surface is the most negative corresponding to the strongest interactions between them. The formation energy of oxygen vacancies (Ov) on the {100} polar plane is also negative, and the abundant Ov facilitates the adsorption of NO2. The internal electric field near the polar surface promotes the charge separation and accelerates the charge exchange between NO2 and the composites. In addition, graphene promotes electron transfer at the interface and improves the stability of the CeO2{100} polar surface. The composites of graphene and metal oxides with a polar surface are excellent for NO2 detection, and the discovery reveals a new sensing strategy.

4.
Theriogenology ; 141: 62-67, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31518730

RESUMO

Primordial germ cells (PGCs) are precursors of sperms and oocytes and responsible for passing the genetic information from one generation to the next. Chicken PGCs segregate from somatic cells in early embryo and could be isolated and cultured in vitro, making it a useful tool to produce genetically modified animals. However, the number of PGCs isolated from embryo is limited and these cells are not efficient to proliferation in vitro. GSK-3 plays an important role in multiple intracellular signaling pathways and inhibition of GSK-3-mediated ß-catenin phosphorylation is known to reduce apoptosis and promote proliferation in T cells and embryo stem cells (ESC). In this study, we investigate the effect of GSK-3 inhibitor on the proliferation of PGCs in vitro and found significant increases of cell proliferation in the culture supplemented with CHIR. We further found that CHIR regulates PGC cell cycle by activating Wnt signaling and antagonizing the apoptosis of PGCs by inhibition of the expression of caspase-3 and Beclin-1. PGCs treated with CHIR expressed the germ cell-related markers and retain the capability to colonize the embryonic gonad after re-introduction to vasculature of HH stage-15 embryos. These results suggest that GSK-3 is involved in cell renewal and apoptosis in chicken PGCs.


Assuntos
Embrião de Galinha/metabolismo , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Células Germinativas/metabolismo , Quinase 3 da Glicogênio Sintase/metabolismo , Piridinas/farmacologia , Pirimidinas/farmacologia , Animais , Células Cultivadas , Quinase 3 da Glicogênio Sintase/antagonistas & inibidores , Quinase 3 da Glicogênio Sintase/genética , Via de Sinalização Wnt/efeitos dos fármacos
5.
BMJ Open ; 9(10): e025524, 2019 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-31630095

RESUMO

OBJECTIVE: According to several studies, liver enzymes levels are associated with fasting plasma glucose (FPG) levels. However, the association stratified by body mass index (BMI) remains to be elucidated, especially in Southern China. Therefore, the aim of this study was to investigate the correlation between liver enzymes levels and FPG levels stratified by BMI in Southern China. DESIGN: Cross-sectional study. PARTICIPANTS AND SETTING: 3056 individuals participated in real-time interviews and blood tests in Southern China. Participants were divided into three groups (underweight, normal weight and overweight or obesity) based on BMI cut-offs. MAIN OUTCOME MEASURED: Partial correlation analysis was performed to investigate the relationship between FPG levels and liver tests. Multivariate logistic regression analyses were applied to calculate the adjusted ORs for FPG levels based on liver enzymes levels. RESULTS: There was no association between liver enzymes and FPG either in the underweight group or in the normal weight group; however, a significant correlation was observed in the overweight or obesity group (alanine transaminase (ALT), p<0.01; aspartate aminotransferase (AST), p<0.05). After adjusting for confounding factors, the highest tertiles of ALT still remained significantly positively related to FPG levels in the overweight or obesity group, with an OR of 2.205 (95% CI 1.442 to 3.371) for the 5.56≤FPG<7.00 mmol/L vs the FPG<5.56 mmol/L group and with an OR of 2.297 (95% CI 1.017 to 5.187) for the FPG≥7.00 mmol/L vs the FPG<5.56 mmol/L group, but this correlation was not found for AST. CONCLUSIONS: The association of liver enzymes levels with FPG levels differed based on different BMI cut-offs. ALT levels were significantly positively associated with FPG levels in the overweight or obesity group, but not in the other two groups; AST levels were not associated with FPG levels in any group.

6.
Reprod Domest Anim ; 54(12): 1574-1582, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31544277

RESUMO

The objective of this study was to investigate the effects of different growth factors on the proliferation of Bama mini-pig spermatogonial stem cells (SSCs) in vitro. The growth factors glial cell line-derived neurotrophic factor (GDNF), leukaemia inhibitory factor (LIF), GDNF family receptor alpha-1 (GFRα1) and basic fibroblast growth factor (bFGF) were investigated. The SSCs were seeded on SIM mouse embryo-derived thioguanine- and ouabain-resistant (STO) feeder layers. Cultivation of the cells were subjected to a factorial design of the growth factors GDNF + bFGF, GDNF + bFGF + GFRα1, LIF + bFGF and LIF + bFGF + GFRα1. The SSCs could propagate for 25 passages in the medium adding GDNF + bFGF + GFRα1, 22 passages in the medium adding GDNF + bFGF, 6 passages in the medium adding LIF + bFGF, or LIF + bFGF + GFRα1. qRT-PCR analysis showed that the highest mRNA expression levels of NANOG, POU5F, DDX4, GFRα1 and UCHL1 were detected in the group adding GDNF + bFGF + GFRα1. The SSCs from the group adding GDNF + bFGF + GFRα1 also showed UCHL1-, DBA- and CDH1-positive staining. Moreover, Stra8 and Scp3 expression, and haploid peak were detected after induction of the SSCs from the group adding GDNF + bFGF + GFRα1. In conclusion, pig SSCs could be maintained for long term in the presence of GDNF, bFGF, and GFRα1.

7.
Zhongguo Zhen Jiu ; 39(6): 605-8, 2019 Jun 12.
Artigo em Chinês | MEDLINE | ID: mdl-31190496

RESUMO

OBJECTIVE: To explore and quantify the intervention effect of auricular point sticking on perioperative psychological stress in patients with anorectal diseases. METHODS: Eighty patients who underwent anorectal surgery were randomly divided into an observation group (40 cases) and a control group (40 cases). The routine preoperative guidance, preoperative visits, and informed of the postoperative condition were received in the control group. On the basis of the treatment in the control group, auricular point sticking was immediately applied at Shenmen (TF4), Shen (CO10), Wei (CO4), Gan (CO12), Pi (CO13), Pizhixia (AT4), E (AT1), Nie (AT2) and Zhen (AT3) in the observation group.The patients were pressed by themselves, 3 to 5 min per point each time, 5 times a day, and the contralateral auricular points were replaced every 2 or 3 days until 1 week after surgery. The Hamilton anxiety scale (HAMA), Hamilton depression scale (HAMD), and Pittsburgh sleep quality index (PSQI) scores were compared between the two groups before and 7 days after surgery. RESULTS: There was no significant difference in the total HAMA scores between after and before surgery in the observation group (P>0.05). The total HAMA score in the control group was higher than that before surgery (P<0.05). The total HAMA score in the observation group after surgery was lower than that in the control group (P<0.05). There was no significant difference in the total HAMD scores between the two groups before and after surgery (P<0.05). There was no significant difference in the total HAMD scores between the two groups after the surgery (P>0.05). The scores of somatic anxiety factor in the two groups were higher than those before surgery (P<0.05). The scores of somatic anxiety factor in the observation group were lower than those in the control group (P<0.05). The scores of psychotic anxiety factors in the two groups were lower than those before surgery (P<0.05). There was no significant difference in the score of psychotic anxiety factors between the two groups (P>0.05). The total score of PSQI in the two groups was lower than that before surgery (P<0.05), and the total score of PSQI in the observation group was lower than that in the control group (P<0.05). CONCLUSION: Auricular point sticking can effectively improve some psychological stress problems during perioperative period in patients with anorectal diseases.


Assuntos
Acupuntura Auricular , Transtornos de Ansiedade , Doenças Retais , Pontos de Acupuntura , Transtornos de Ansiedade/terapia , Humanos , Doenças Retais/cirurgia , Estresse Psicológico
8.
Reprod Domest Anim ; 54(9): 1195-1205, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31228864

RESUMO

As a natural plant-derived antitoxin, resveratrol possesses several pharmacological activities. This study aimed to evaluate the effects of resveratrol addition on nuclear maturation, oocyte quality during in vitro maturation (IVM) of porcine oocytes and subsequent early embryonic development following somatic cell nuclear transfer (SCNT). Our experiments showed that the treatment of porcine oocytes with 5 µM resveratrol during IVM resulted in the highest rate of the first polar body extrusion. Treatment of oocytes with resveratrol had no influence on cytoskeletal dynamics, whereas it significantly increased glucose uptake ability compared to the control oocytes. Oocytes matured with 5 µM resveratrol displayed significantly lower intracellular reactive oxygen species (ROS) levels and higher relative mRNA expression levels of the genes encoding such antioxidant enzymes as catalase (CAT) and superoxide dismutase 1 (SOD1). In addition, resveratrol also prevented onset and progression of programmed cell death in porcine oocytes, which was confirmed by significant upregulation of the anti-apoptotic B-cell lymphoma 2 (BCL-2) gene and significant downregulation of the pro-apoptotic BCL2-associated X (BAX) gene. Furthermore, the blastocyst rates and the blastocyst cell numbers in cloned embryos derived from the oocytes that had matured in the presence of 5 µM resveratrol were significantly increased. In conclusion, supplementation of IVM medium with 5 µM resveratrol improves the quality of porcine oocytes by protecting them from oxidative damage and apoptosis, which leads to the production of meiotically matured oocytes exhibiting enhanced developmental potential following SCNT.


Assuntos
Desenvolvimento Embrionário/efeitos dos fármacos , Técnicas de Maturação in Vitro de Oócitos/veterinária , Técnicas de Transferência Nuclear/veterinária , Resveratrol/farmacologia , Animais , Antioxidantes/metabolismo , Antioxidantes/farmacologia , Apoptose/efeitos dos fármacos , Blastocisto , Feminino , Regulação da Expressão Gênica no Desenvolvimento , Genes bcl-2 , Técnicas de Maturação in Vitro de Oócitos/métodos , Oócitos/efeitos dos fármacos , RNA Mensageiro , Espécies Reativas de Oxigênio/metabolismo , Suínos , Proteína X Associada a bcl-2/genética
9.
ACS Appl Mater Interfaces ; 11(13): 12958-12967, 2019 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-30848880

RESUMO

Although high-energy facets on metal oxides are usually active and preferred for gas sensing, it is difficult to expose them according to thermodynamics. In this work, nanocomposites of SnO2 and graphene are prepared by a hydrothermal method. The SnO2 nanoparticles change from a lance shape to an octahedral shape as the concentration of HCl in the solution is increased gradually from 6.5 to 10 vol %. However, the SnO2 nanoparticles have an elongated octahedral shape if the concentration of HCl is increased further. The octahedral SnO2 nanoparticles are mainly surrounded by high-surface-energy {221} facets, thus facilitating gas sensing. First-principles calculation shows that the surface energy and adsorption energy of the {221} facets are larger than those of the stable {110} facets, and so, the gas adsorption capacity on the {221} facets is better. Furthermore, because the Fermi level of the SnO2{221} facet is higher than that of graphene, the electrons are transferred from SnO2 nanoparticles to graphene sheets, enabling effective electron exchange between the composite and external NO2 gas. The excellent gas-sensing properties of the octahedral SnO2/graphene composites are ascribed to the high-surface-energy {221} facets exposed.

10.
Theriogenology ; 123: 83-89, 2019 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-30292859

RESUMO

Primordial germ cells (PGCs) are promising genetic resources for avian studies including modified animals. However, chicken PGCs are slow to proliferate and gradually lose germline competency after long-term culture, which hinders their application in avian biotechnology. Thus, we developed a robust method for the isolation and rapid propagation of PGCs using an indirect co-culture system. PGCs derived from a pair of embryonic chicken gonads were expanded to 1 × 106 within 2 weeks, and no sex bias was observed in. These PGCs presented high capacity of germline transmission and produced donor-derived offspring after injection into the chicken embryos. This system allows the efficient gene-banking of chicken species and can facilitate the production of chickens bearing a desired phenotype via genomic editing.


Assuntos
Técnicas de Cocultura/veterinária , Células Germinativas/fisiologia , Animais , Diferenciação Celular/fisiologia , Células Cultivadas , Embrião de Galinha , Criopreservação/veterinária , Feminino , Gônadas/citologia , Gônadas/embriologia , Masculino
11.
Reprod Fertil Dev ; 31(2): 386-394, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30309436

RESUMO

The effects of acetyl-l-carnitine (ALC) supplementation during IVM on subsequently vitrified buffalo oocytes were evaluated, followed by determination of the mitochondrial DNA copy number, measurement of mitochondrial membrane potential (MMP) and identification of the lipid profile of oocyte membranes as markers of oocyte quality after vitrification. Supplementation with ALC during IVM significantly improved the rates of oocyte cleavage and morula and blastocyst formation, and increased MMP after vitrification compared with unsupplemented vitrified oocytes (P<0.05). Using a bidirectional orthogonal projection to latent structures discriminant analysis based on positive ion matrix-assisted laser desorption ionisation time-of-flight mass spectrometry data, five phospholipid ions (m/z 728.7 (phosphatidylcholine (PC) 32:3), 746.9 (PC 32:5), 760.6 (PC 34:1), 768.8 (PC P-36:3) and 782.6 (PC 36:4); P<0.05) were identified as significantly more abundant in fresh oocytes than in unsupplemented vitrified oocytes. Meanwhile, three phospholipid ions (m/z 734.6 (PC 32:0), 760.6 (PC 34:1), and 782.6 (PC 36:4); P<0.05) were more abundant in ALC-supplemented vitrified oocytes than in unsupplemented vitrified oocytes. Therefore, supplementation with ALC during IVM may improve buffalo oocyte quality after vitrification by enhancing mitochondrial function and altering the phospholipid composition of vitrified oocyte membranes.


Assuntos
Acetilcarnitina/farmacologia , Desenvolvimento Embrionário/efeitos dos fármacos , Lipídeos de Membrana/metabolismo , Mitocôndrias/efeitos dos fármacos , Oócitos/efeitos dos fármacos , Animais , Búfalos , Criopreservação/métodos , Feminino , Técnicas de Maturação in Vitro de Oócitos , Mitocôndrias/metabolismo , Oócitos/metabolismo , Vitrificação
12.
Aging (Albany NY) ; 10(12): 3897-3909, 2018 12 10.
Artigo em Inglês | MEDLINE | ID: mdl-30530915

RESUMO

Prolonged culture of metaphase II oocytes is an in vitro aging process that compromises oocyte quality. We tested whether melatonin preserves epigenetic modifications in oocytes after prolonged culture. The porcine oocytes were maturated in vitro for 44 h, and then metaphase II oocytes were continuously cultured in medium supplemented with or without melatonin for 24 h. We found that the parthenogenetic blastocyst formation rate of prolonged-culture oocytes was lower than in fresh oocytes. We further observed that methylation at H3K4me2 and H3K27me2 of oocytes enhanced after prolonged culture. However, 5mc fluorescence intensity was lower in prolonged-culture oocytes than in fresh oocytes. Moreover, the promoter of the imprinted gene NNAT exhibited a higher level of DNA methylation in prolonged-culture oocytes than in fresh oocytes, which was associated with a reduced expression level and glucose uptake capability. Conversely, melatonin improved blastocyst formation rate and preserved histone and DNA methylation modifications, as well as NNAT function in the oocytes after prolonged culture. Notably, DNA methyltransferase inhibitor 5-aza significantly attenuated the protective role of melatonin on genomic DNA methylation. In summary, our results revealed that epigenetic modifications are disrupted in oocytes after prolonged culture, but the changes are reversed by melatonin.


Assuntos
Epigênese Genética/efeitos dos fármacos , Melatonina/farmacologia , Oócitos/efeitos dos fármacos , Oócitos/fisiologia , Suínos , Animais , Técnicas de Cultura de Células , Metilação de DNA , Regulação da Expressão Gênica/efeitos dos fármacos , Glucose/metabolismo , Técnicas de Maturação in Vitro de Oócitos/veterinária , Preservação de Tecido
13.
J Vet Sci ; 19(5): 592-599, 2018 09 30.
Artigo em Inglês | MEDLINE | ID: mdl-29929354

RESUMO

In this study, we attempted to establish a culture system for in vitro spermatogenesis from spermatogonial stem cells (SSCs) of Bama mini-pig. Dissociated testicular cells from 1-month-old pigs were co-cultured to mimic in vivo spermatogenesis. The testicular cells were seeded in minimum essential medium alpha (α-MEM) supplemented with Knockout serum replacement (KSR). Three-dimensional colonies formed after 10 days of culture. The colonies showed positive staining for SSC-associated markers such as UCHL1, PLZF, THY1, OCT4, Dolichos biflorus agglutinin, and alkaline phosphatase. Induction of SSCs was performed in α-MEM + KSR supplemented with retinoic acid, bone morphogenetic protein 4, activin A, follicle-stimulating hormone, or testosterone. The results showed that STRA8, DMC1, PRM1, and TNP1 were upregulated significantly in the colonies after induction compared to that in testis from 1-month-old pigs, while expression levels of those genes were significantly low compared to those in 2-month-old testis. However, upregulation of ACROSIN was not significant. Replacement of α-MEM and KSR with Iscove's modified Dulbecco's medium and fetal bovine serum did not upregulate expression of these genes significantly. These results indicate that SSCs of Bama mini-pig could undergo differentiation and develop to a post-meiotic stage in α-MEM supplemented with KSR and induction factors.


Assuntos
Células-Tronco Germinativas Adultas/metabolismo , Técnicas de Cultura de Células/veterinária , Diferenciação Celular/fisiologia , Espermatogênese , Porco Miniatura/metabolismo , Testículo/citologia , Animais , Técnicas de Cultura de Células/métodos , Técnicas In Vitro , Masculino , Suínos
14.
Theriogenology ; 118: 80-89, 2018 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-29885644

RESUMO

Oocyte quality is one of the important factors in female fertility, in vitro maturation (IVM), and subsequent embryonic development. In the present study, we assessed whether acetyl-l-carnitine (ALC) supplementation during in vitro maturation of buffalo oocytes could improve oocyte quality and subsequent embryonic development. To determine the optimal level of ALC supplementation, we matured cumulus-oocyte complexes in maturation medium supplemented with 0, 2.5, and 5 mM ALC. The oocytes with a polar body were selected for parthenogenetic activation (PA) and in vitro fertilization (IVF). We found that oocytes matured in 2.5 mM ALC had significantly higher PA blastocyst rate (P < 0.05) and blastocyst cell number than those of unsupplemented oocytes (P < 0.05) and a significantly higher IVF blastocyst rate than that of oocytes matured in 5 mM ALC (P < 0.05). In all further experiments, we supplemented the maturation medium with 2.5 mM ALC. We then tested whether ALC supplementation could improve various markers of oocytes and cumulus cells. We compared cell proliferation; concentrations of reactive oxygen species (ROS), intracellular ATP, estradiol, and progesterone; mitochondrial distribution; mitochondrial DNA copy number (mtDNA); and expression levels of four genes encoding oocyte-derived factors (GDF9, BMP15) and steroid hormones (StAR, P450scc) between the supplemented and unsupplemented oocytes and cumulus cells. Cumulus cells matured with ALC supplementation were more prolific than those matured without ALC supplementation (P < 0.05). Oocytes treated with ALC had lower concentrations of intracellular ROS (P < 0.05) and a higher rate of diffuse mitochondrial distributions (P < 0.05) than those of untreated oocytes. Additionally, the mtDNA was higher in the ALC-treated oocytes (P < 0.05) and cumulus cells (P < 0.05) than that in the untreated cells. The ALC-treated maturation medium had a higher postmaturation concentration of estradiol than that of the untreated medium (P < 0.05). Finally, the gene expression levels of P450scc and GDF9 were greater in ALC-treated oocytes and cumulus cells than those in untreated cells (P < 0.05). Therefore, in buffalo, our results suggest that ALC affects mitochondrial function, regulates oocyte-derived paracrine factors, and increases the production of steroid hormones, leading to increased quality of matured oocytes and improved embryonic development in vitro.


Assuntos
Acetilcarnitina/farmacologia , Búfalos , Desenvolvimento Embrionário/efeitos dos fármacos , Técnicas de Maturação in Vitro de Oócitos/veterinária , Oócitos/efeitos dos fármacos , Acetilcarnitina/administração & dosagem , Animais , Blastocisto/fisiologia , Proliferação de Células/efeitos dos fármacos , Meios de Cultura , Células do Cúmulo/efeitos dos fármacos , Células do Cúmulo/fisiologia , DNA Mitocondrial/análise , Desenvolvimento Embrionário/fisiologia , Estradiol/análise , Feminino , Fertilização In Vitro/veterinária , Técnicas de Maturação in Vitro de Oócitos/métodos , Oócitos/química , Oócitos/fisiologia , Espécies Reativas de Oxigênio/análise
15.
Mol Med Rep ; 17(6): 8466-8474, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29693163

RESUMO

It is widely accepted that infusion of mesenchymal stem cells (MSCs) ameliorates hyperglycemia by alleviating insulin resistance in rats with type 2 diabetes mellitus (T2D). However, the detailed underlying mechanisms are not clearly defined. Mitsugumin 53 (MG53) is an E3 ligase that has recently been implicated in the aggravation of insulin resistance by promoting the ubiquitinoylation of insulin receptor substrate­1 (IRS­1) in skeletal muscles. It was therefore hypothesized that MG53 may be involved in MSC­mediated therapeutic effects on insulin resistance. To test this hypothesis, in the present study, T2D rat models were induced by a high­fat diet combined with streptozotocin administration and MSC infusion was performed four times (once every 2 weeks for 8 weeks). The therapeutic effects of MSC infusion on insulin resistance were evaluated and the effect on the expression of MG53 and insulin receptor signaling elements in skeletal muscle was also investigated by immunofluorescence staining and western blotting. The results demonstrated that MSC infusion ameliorated hyperglycemia and insulin resistance in T2D rats. Furthermore, MSC infusion inhibited MG53 elevation and reversed the decreases in glucose transporter type 4, insulin receptor, IRS­1 and phosphorylated­AKT levels in the skeletal muscle of T2D rats. These results indicated that MSC infusion has therapeutic effects in rats and that MG53 in skeletal muscle may be a promising novel therapeutic target protein for MSC­mediated amelioration of insulin resistance in T2D.


Assuntos
Tecido Adiposo/citologia , Diabetes Mellitus Tipo 2/metabolismo , Resistência à Insulina , Insulina/metabolismo , Células-Tronco Mesenquimais/metabolismo , Proteínas Musculares/metabolismo , Proteínas de Transporte Vesicular/metabolismo , Animais , Células Cultivadas , Diabetes Mellitus Experimental , Modelos Animais de Doenças , Glucose/metabolismo , Masculino , Células-Tronco Mesenquimais/citologia , Músculo Esquelético/metabolismo , Ratos , Reprodutibilidade dos Testes
16.
Adv Mater ; 30(10)2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29349814

RESUMO

Vanadium dioxide/titanium nitride (VO2 /TiN) smart coatings are prepared by hybridizing thermochromic VO2 with plasmonic TiN nanoparticles. The VO2 /TiN coatings can control infrared (IR) radiation dynamically in accordance with the ambient temperature and illumination intensity. It blocks IR light under strong illumination at 28 °C but is IR transparent under weak irradiation conditions or at a low temperature of 20 °C. The VO2 /TiN coatings exhibit a good integral visible transmittance of up to 51% and excellent IR switching efficiency of 48% at 2000 nm. These unique advantages make VO2 /TiN promising as smart energy-saving windows.

17.
J Mol Med (Berl) ; 96(1): 97-109, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-29116339

RESUMO

Despite substantial progress being made in understanding the mechanisms contributing to the pathogenesis of renal fibrosis, there are only a few therapies available to treat or prevent renal fibrosis in clinical use today. Therefore, identifying the key cellular and molecular mediators involved in the pathogenesis of renal fibrosis will provide new therapeutic strategy for treating patients with chronic kidney disease (CKD). ß-Arrestin-1, a member of ß-arrestin family, not only is a negative adaptor of G protein-coupled receptors (GPCRs), but also acts as a scaffold protein and regulates a diverse array of cellular functions independent of GPCR activation. In this study, we identified for the first time that ß-arrestin-1 was upregulated in the kidney from mice with unilateral ureteral obstruction nephropathy as well as in the paraffin-embedded sections of human kidneys from the patients with diabetic nephropathy, polycystic kidney, or uronephrosis, which normally causes renal fibrosis. Deficiency of ß-arrestin-1 in mice significantly alleviated renal fibrosis by the regulation of inflammatory responses, kidney fibroblast activation, and epithelial-mesenchymal transition (EMT) in both in vivo and in vitro studies. Furthermore, we found that among the major isoforms of Wnts, Wnt1 was regulated by ß-arrestin-1 and gene silencing of Wnt1 inhibited the activation of ß-catenin and suppressed ß-arrestin-1-mediated renal fibrosis. Collectively, our results indicate that ß-arrestin-1 is one of the critical components of signal transduction pathways in the development of renal fibrosis. Modulation of these pathways may be an innovative therapeutic strategy for treating patients with renal fibrosis. KEY MESSAGES: ß-Arrestin-1 was upregulated in the kidney from mice with UUO nephropathy. ß-Arrestin-1 regulated kidney fibroblast activation and epithelial-mesenchymal transition. ß-Arrestin-1 exacerbated renal fibrosis via mediating Wnt1/ß-catenin signaling.


Assuntos
Nefropatias/metabolismo , Via de Sinalização Wnt , beta-Arrestina 1/metabolismo , Animais , Linhagem Celular , Transição Epitelial-Mesenquimal , Fibrose , Humanos , Rim/metabolismo , Rim/patologia , Nefropatias/genética , Masculino , Camundongos Endogâmicos C57BL , Camundongos Knockout , Fator de Crescimento Transformador beta1/farmacologia , Regulação para Cima , Proteína Wnt1/genética , beta Catenina/metabolismo , beta-Arrestina 1/genética
18.
Anim Reprod Sci ; 186: 44-51, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28982519

RESUMO

Nanos2 belongs to the Nanos gene-coding family and is an important RNA-binding protein that has been shown to have essential roles in male germline stem cells development and self-renewal in mouse. However, little is known about Nanos2 in inchoate buffalo spermatogonia. Here, rapid-amplification of cDNA ends (RACE) was used to obtain the full-length buffalo Nanos2 sequence and bioinformatic analysis revealed a highly conserved Nanos2 sequence between buffalo and other mammalian species. Although Nanos2 was expressed in various tissues, the highest mRNA expression levels were found in testes tissue. Moreover, Nanos2 mRNA was abundant in fetal and pre-puberal testes but markedly decreased in the testes of adults. At the protein level, immunohistochemistry in pre-puberal testes revealed a pattern of NANOS2 expression similar to that for the undifferentiated type A spermatogonia marker PGP9.5. Furthermore, NANOS2 expression was low in adult testes and restricted to elongating spermatids. Altogether, our data suggest that Nanos2 is a potential preliminary molecular marker of inchoate buffalo spermatogonia, and may play an important role in buffalo spermatogonial stem cells (SSCs) development and self-renewal, as has been observed in other model animals.


Assuntos
Búfalos/genética , Marcadores Genéticos , Proteínas de Ligação a RNA/genética , Espermatogônias/fisiologia , Animais , Búfalos/crescimento & desenvolvimento , Clonagem Molecular , Biologia Computacional , Regulação da Expressão Gênica no Desenvolvimento , Masculino , Maturidade Sexual , Testículo/crescimento & desenvolvimento
19.
Medicine (Baltimore) ; 96(34): e7869, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28834901

RESUMO

Rhegmatogenous retinal detachment associated with choroidal detachment (RRDCD) is a special type of complex retinal detachment, and usually has a poor prognosis. This study aimed to assess the anatomical outcomes of 23-gauge pars plana vitrectomy (23G PPV) combined with phacoemulsification (phaco) and capsulotomy without intraocular lens (IOL) implantation in patients with RRDCD.Seventy-six consecutive patients with RRDCD, who underwent retinal repair surgery from January 2010 to December 2014, were retrospectively analyzed. Forty patients underwent 23G PPV + phaco + IOL implantation, and 36 underwent 23G PPV + phaco + capsulotomy without IOL implantation (i.e., aphakia). All cases were filled with silicone oil. The follow-up time was 6 months after silicone oil was removed. Multivariate logistic regression analysis was the statistical method used.The overall retinal anatomical reattachment rate was 58% (44/76): 40% (16/40) of patients receiving 23G PPV + phaco + IOL implantation; and 78% (28/36) of patients receiving 23G PPV + phaco + capsulotomy + aphakia (P = .007).Surgical repair using 23G PPV + phaco + capsulotomy without IOL implantation can improve anatomical reattachment rates in patients with RRDCD.


Assuntos
Doenças da Coroide/cirurgia , Facoemulsificação/métodos , Capsulotomia Posterior/métodos , Descolamento Retiniano/cirurgia , Vitrectomia/métodos , Adolescente , Adulto , Idoso , Doenças da Coroide/complicações , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Descolamento Retiniano/complicações , Estudos Retrospectivos , Adulto Jovem
20.
Dalton Trans ; 46(13): 4174-4181, 2017 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-28252139

RESUMO

One-dimensional tellurides are good candidates for thermoelectric applications, but the fabrication of telluride nanotubes is still challenging. To this end, the solvothermal approach is proposed to synthesize Bi2Te3, PbTe, CuxTe and Ag2Te nanotubes. In this scheme, single-crystal Te nanotubes are produced first and then used as the sacrificed template for epitaxial growth of metal telluride. It was demonstrated that polycrystalline telluride nanotubes are produced. Considering Bi2Te3 nanotubes as an example, the pellets are prepared by spark plasma sintering, and the thermoelectric properties are measured. Compared to the nanowire counterpart, the higher-energy barrier to electrons at the grain boundaries (GBs) leads to an optimized power factor of 1.04 mW m-1 K-2 at 373 K in the nanotube samples. Furthermore, the thermal conductivity of nanotubes is in the range of 0.503-0.617 W m-1 K-1, which is much smaller than that of the nanowires. The ultralow thermal conductivity could be attributed to both the higher potential barrier of GBs and the additional scattering of phonons at the side walls of the nanotubes. In all, a ZT value of 0.74 was obtained at 373 K, which is much higher than that of nanowires. This synthesis route is ready to be extended to other telluride nanotubes.

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