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1.
Biomed Pharmacother ; 127: 110155, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32413669

RESUMO

Dual-PPAR-α/γ agonist has the dual potentials to improve insulin resistance (IR) and hepatic steatosis associated with obesity. This study aimed to investigate whether dehydroabietic acid (DA), a naturally occurred compound, can bind to and activate both PPAR-γ and PPAR-α to ameliorate IR and hepatic steatosis in high-fat diet (HFD)-fed mice.. We found that DA formed stable hydrogen bonds with the ligand-binding domains of PPAR-γ and PPAR-α. DA treatment also promoted 3T3-L1 differentiation via PPAR-γ activation, and mitochondrial oxygen consumption in HL7702 cells via PPAR-α activation. In HFD-fed mice, DA treatment alleviated glucose intolerance and IR, and reduced hepatic steatosis, liver injury markers (ALT, AST), and lipid accumulation, and promoted mRNA expression of PPAR-γ and PPAR-α signaling elements involved in IR and lipid metabolism in vivo and in vitro, and inhibited mRNA expression of pro-inflammatory factors. Therefore, DA is a dual-PPAR-α/γ and PPAR-γ partial agonist, which can attenuate IR and hepatic steatosis induced by HFD-consumption in mice.

2.
Medicine (Baltimore) ; 99(16): e19755, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32311975

RESUMO

Although proliferating cell nuclear antigen (PCNA) plays an important role in tumor proliferation and its expression level is closely related to the biological activity of tumor cells, PCNA expression in non-small cell lung cancer (NSCLC) has been seldom reported. In this study, we aimed to investigate the significance of PCNA expression in NSCLC tissues. PCNA expression in NSCLC and adjacent tissues were assessed by immunohistochemistry (IHC), western blotting, and reverse transcription polymerase chain reaction. Single factor analysis was used to study the relationship between the expression of PCNA and clinicopathological features of NSCLC. Multi-factor Cox survival analysis was used to evaluate the relationship between the expression of PCNA and overall survival of postoperative NSCLC patients. The areas under the receiver operating characteristics were calculated to evaluate the value of PCNA expression level in predicting the 3-year survival of NSCLC patients. IHC analysis showed that the positive expression rates of PCNA protein in NSCLC and adjacent tissues were 91.79% (257/280) and 25.83% (31/120), respectively. Western blotting confirmed that PCNA protein level was significantly higher in NSCLC tissues than in the adjacent tissues (P < .05). Reverse transcription polymerase chain reaction showed that the positive rate of PCNA mRNA in NSCLC was 88.93% (249/280), which was significantly higher than that in adjacent tissues 29.17% (35/120) (P < .05). Both PCNA mRNA and protein levels were correlated with tumor differentiation, size, metastasis, and stage in NSCLC. Patients exhibiting higher PCNA protein expression had a significantly shorter disease-specific survival rate than the other patients. PCNA protein level and tumor pathological type, metastasis, differentiation degree, and stage were independent factors affecting the overall survival of postoperative patients. The areas under the receiver operating characteristics of PCNA mRNA for predicting the 3-year survival of NSCLC patients was 0.89 (0.79-0.98), with a sensitivity and specificity of 0.84 and 0.76, respectively. In conclusion, high PCNA protein and mRNA levels may be associated with the occurrence, development, and prognosis of NSCLC.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/metabolismo , Neoplasias Pulmonares/metabolismo , Antígeno Nuclear de Célula em Proliferação/metabolismo , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores Tumorais/metabolismo , Carcinoma Pulmonar de Células não Pequenas/mortalidade , Carcinoma Pulmonar de Células não Pequenas/cirurgia , China/epidemiologia , Estudos Transversais , Feminino , Humanos , Neoplasias Pulmonares/mortalidade , Neoplasias Pulmonares/cirurgia , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos
3.
Environ Sci Pollut Res Int ; 27(8): 8248-8259, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31900778

RESUMO

A facile one-pot solvent thermal method was proposed to synthesize magnetic mesoporous carbon (MMC) using Fe(NO3)3·9H2O as a precursor, Pluronic copolymer P123 as template, and chitosan as carbon source, and it was applied for the adsorptive remediation of methyl orange (MO). The characterization results of TEM, XRD, and IR showed that MMC consisted of graphitized carbon matrix and some black spherical particle mixture of Fe3O4 and Fe, and it was rich in hydroxyl and carbonyl groups. Besides, the effect of the content of Fe and the content of chitosan in MMC on the magnetism and adsorption performance of prepared material were investigated. In addition, the effects of pH value, initial concentration of methyl orange, and contact time on the adsorption performance of MO were studied, respectively. At 318 K, the maximum adsorption capacity of MO calculated from Langmuir isotherm was from 139 to 400 mg g-1 on MMC. Kinetic studies demonstrated that the adsorption process obeyed a pseudo-second-order kinetic model. The regeneration experiments revealed that MMC could be reused at least five times without notable decrease of adsorption performance. These results illustrate that MMC is an efficient and economical adsorbent for the adsorption of MO.


Assuntos
Compostos Azo/química , Carbono , Fenômenos Magnéticos , Adsorção , Carbono/química , Concentração de Íons de Hidrogênio , Cinética
4.
Biomed Pharmacother ; 124: 109886, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32000045

RESUMO

This study aims to investigate whether magnolol (MG), a natural neolignane compound, can prevent AD induced by beta-amyloid (Aß) and the possible mechanisms involved. MG dose-dependently reduces Aß deposition, toxicity and memory impairment caused by Aß in transgenic C. elegans. More importantly, these effects are reversed by GW9662, a selective peroxisome proliferator-activated receptor-γ (PPAR-γ) antagonist. MG is more effective in enhancing PPAR-γ luciferase levels than honokiol (HK). Meanwhile, MG has the potential to bind with the ligand binding domain of PPAR-γ (PPAR-γ-LBD). As expected, MG inhibited the luciferase activity of NF-κB and its target genes of inflammatory cytokines, and this effect was blocked by GW9662. The luciferase activity of Nrf2-ARE expression can be activated by MG and decreased Aß-induced reactive oxygen species (ROS). The target gene LXR of PPAR-γ is activated by MG, which upregulates ApoE and promotes microglia phagocytosis and the degradation of Aß, and these effects were also reversed by GW9662. In summary, MG can attenuate Aß-induced AD and the underlying mechanism is the reduction of inflammation and promotion of phagocytosis and degradation of Aß, which is dependent on PPAR-γ.

5.
Neurosci Lett ; 716: 134633, 2020 01 18.
Artigo em Inglês | MEDLINE | ID: mdl-31743752

RESUMO

Kaempferol is a medicinal flavonol derived from the roots of Kaempferia galanga L. Kaempferol can affect cell survival, apoptosis, and anti-oxidation, though its role and underlying mechanism in retinal ganglion cells with high-glucose injury remains unclear. In this study, we explored kaempferol's role in high-glucose injury in cells from the retinal ganglion cell (RGC) line. RGC cells were isolated and then cultured in high glucose (55 mmol/L) for 0 h, 12 h, 24 h, 48 h, or 72 h, and results showed decreased cell viability at 48 h and 72 h. We treated RGC cells with different concentrations of kaempferol (0 µmol/L, 20 µmol/L, 40 µmol/L, 60 µmol/L, 80 µmol/L, or 100 µmol/L) and high-glucose (55 mmol/L) for 48 h. The data indicated inhibited lactate dehydrogenase leakage, apoptosis, caspase-3 activity, and reactive oxygen species (ROS) levels. Moreover, whereas cell viability increased in RGC cells that were incubated with kaempferol (60 µmol/L, 80 µmol/L, or 100 µmol/L) and glucose (55 mmol/L), compared with glucose alone. Kaempferol (60 µmol/L) elevated ERK phosphorylation and vasohibin-1 (VASH1) expression, and inhibition of ERK phosphorylation reversed the effect of kaempferol (60 µmol/L) on VASH1 expression in RGC cells with high-glucose injury. Additionally, interference of VASH1 by VASH1 siRNA markedly reversed the effects of kaempferol (60 µmol/L) on cell viability, caspase-3 activity, and ROS levels in RGC cells with high glucose injury. Taken together, the results suggest that kaempferol protected retinal ganglion cells from high-glucose-induced injury via ERK and VASH1 signaling.

6.
Dalton Trans ; 49(2): 411-417, 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31833493

RESUMO

Two novel interpenetrated 2-fold Mn-based metal-organic frameworks (MOFs) (SC-7 and SC-8), assembled from the rigid ligand H3TATB (4,4',4''-s-triazine-2,4,6-triyl-tribenzoic acid) and Mn ions with the assistance of the flexible N-donor linker BIB (bis((1H-imidazol-1-yl)methyl)benzene) or TIPA (tris(4-imidazolylphenyl)amine), have been successfully prepared. The as-obtained MOFs show two distinct topological structures with the symbols 44·62 and (52·6)(53·6·73·82·9) due to discrepancies between the flexibilities of the bi-imidazole and tri-imidazole linkers. The electrodes based on the as-prepared bulk Mn-MOFs behave as alkaline batteries in electrochemical cells and deliver high capacities (279 and 172 mA h g-1 at 1 A g-1 for SC-7 and SC-8, respectively). Theoretical mechanism analyses indicate that the surface-controlled (k1v) process can be transformed into a diffusion-dominated (k2v1/2) process when the charging time exceeds 30 seconds in the MOF-based systems. Our research provides a new strategy to construct an increasing number of stable redox sites in MOFs for application to battery-capacitor hybrid devices.

7.
Biosci Rep ; 39(4)2019 04 30.
Artigo em Inglês | MEDLINE | ID: mdl-30962272

RESUMO

To investigate the significance of stanniocalcin-2 (STC2) expression in hepatocellular carcinoma (HCC) tissues and adjacent tissues. Levels of STC2 in HCC tissue were detected in 200 HCC patients tissues and adjacent tissues as controls by immunohistochemistry technique (IHC) and reverse transcriptase-PCR (RT-PCR). Single factor analysis was used to study the relationship between expression of STC2 mRNA and protein and clinicopathological features of HCC. Multifactor Cox survival analysis was used to relationship between the expression of STC2 and overall survival of postoperative patients with HCC. IHC staining showed that the expression of STC2 protein rate was 81.00% (163/200). And the positive rate of adjacent tissues was 29.00% (58/200). Western blot showed that the expression of STC2 protein in HCC was significantly higher than that in the adjacent tissues (P<0.05). RT-PCR showed that the positive rates of STC2 mRNA expression in HCC were 75.50% (151/200), which was significantly higher than that in adjacent tissues 14.50% (29/200) (P<0.05). Both STC2 mRNA and protein expression are related to tumor diameter, stage, tumor metastasis, carcinoma emboli in the portal vein and the degree of tumor differentiation in HCC. The HCC patients with higher expression of STC2 had shorter median survival time. STC2 expression, tumor diameter, carcinoma emboli in the portal vein, tumor differentiation degree, and tumor stage were independent factors affecting the overall survival of postoperative patients. The high expression of STC2 mRNA and protein expression in HCC may be associated with the occurrence, development, and prognosis of HCC. STC2 may also be possible to help developing new therapeutic strategies for HCC.


Assuntos
Biomarcadores Tumorais/genética , Carcinoma Hepatocelular/cirurgia , Glicoproteínas/genética , Peptídeos e Proteínas de Sinalização Intercelular/genética , Neoplasias Hepáticas/cirurgia , Adulto , Idoso , Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/patologia , Intervalo Livre de Doença , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/patologia , Masculino , Pessoa de Meia-Idade , Prognóstico
8.
Molecules ; 24(4)2019 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-30781735

RESUMO

Progastrin-releasing peptide (ProGRP), which is known to be highly specific and sensitive to small cell lung cancer (SCLC), has been proven to be a valuable substitute for neuron-specific enolase in SCLC diagnostics and monitoring, especially in its early stages. The detection of ProGRP levels also facilitates a selection of therapeutic treatments. For the fabrication of our proposed biosensor, titanium (IV) oxide microparticles were first used, followed by dispersing gold nanoparticles into chitosan and immobilizing them onto a carbon paste electrode (CPE) surface. The developed immunosensor exhibits a much higher biosensing performance in comparison with current methods, when it comes to the detection of ProGRP. Therefore, the proposed CPE/TiO2/(CS+AuNPs)/anti-ProGRP/BSA/ProGRP is excellent for the development of a compact diagnostics apparatus.


Assuntos
Biomarcadores Tumorais/sangue , Técnicas Biossensoriais , Fragmentos de Peptídeos/sangue , Carcinoma de Pequenas Células do Pulmão/sangue , Ouro/química , Humanos , Nanocompostos/química , Fosfopiruvato Hidratase/genética , Proteínas Recombinantes/sangue , Carcinoma de Pequenas Células do Pulmão/genética , Carcinoma de Pequenas Células do Pulmão/patologia , Titânio/química
9.
Water Sci Technol ; 78(5-6): 1179-1188, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30339542

RESUMO

A facile approach has been developed to construct a composite of magnetic Fe3O4 (MNPs) and regular hexagon Mg-Al layered double hydroxide (MNPs/MgAl-LDH) via a two-step hydrothermal method combined with the urea hydrolysis reaction for the removal of Orange II. The scanning electron microscopy, X-ray diffraction and Fourier transform infrared spectroscopy results showed MNPs and MgAl-LDH have been combined successfully, providing the combination of the superior properties of fast separation and high adsorption capacity. The pH values, contact time, initial dye concentration and temperature were investigated in detail. The kinetics and isotherm study showed the adsorption of Orange II on MNPs/MgAl-LDH obeyed the pseudo-second-order and Langmuir model respectively and the adsorption processes were spontaneous and endothermic in nature. Also, some coexisting anions such as Cl-, NO3-, CO3- and SO42- had no significant effect on the removal of Orange II. The mechanism study revealed that the adsorption of Orange II on MNPs/MgAl-LDH mainly involves surface adsorption through electrostatic force and the layer anion exchange. Moreover, Orange II could be desorbed from MNPs/MgAl-LDH using 100 mg L-1 NaOH and used for four cycles without any adsorption performance loss, demonstrating MNPs/MgAl-LDH prepared in this work could be used as a cost-effective and efficient material for the removal of Orange II.


Assuntos
Compostos de Alumínio/química , Compostos Azo/química , Benzenossulfonatos/química , Óxido Ferroso-Férrico/química , Compostos de Magnésio/química , Águas Residuárias/química , Poluentes Químicos da Água/química , Adsorção , Hidróxidos/química , Cinética , Difração de Raios X
10.
ACS Appl Mater Interfaces ; 10(10): 9104-9115, 2018 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-29446614

RESUMO

Synthesizing kinetically stable coordination polymers (CPs) through ligand functionalization can effectively improve their supercapacitive performances. Herein, we have successfully synthesized three novel and topological Co-CPs by varying the flexible N-donor ligand and inorganic anions, namely, interpenetrated [Co(HTATB)( o-bib)]·H2O, extended two-dimensional (2D) layered Co(HTATB)( m-bib)·2H2O, and three-dimensional (3D) Co(HTATB)( m-bib), where bib is the flexible N-donor bis((1 H-imidazol-1-yl)methyl)benzene linker (where o- and m- refer to ortho and meta positions, respectively) ligand and HTATB is the partial deprotonation mode from 4,4',4″- s-triazine-2,4,6-triyl-tribenzoic acid. Various Co-CPs have been directly applied in the field of supercapacitors. All these framework materials exhibit high capacitance, excellent energy delivery efficiency, and good cycling performance. For instance, the maximum specific capacitance for penetrated 3D networks is 2572 F g-1 at 2.0 A g-1, and the mean energy delivery efficiency is up to 92.7% based on the tested current densities. Compared with extensional 2D layered and 3D networks, the 3D interpenetrated and polythreaded architectures could provide more active sites and thus promote fast charging and discharging processes. Furthermore, the Li+ uptake-release abilities of the Co-based CPs are also investigated, and the initial discharge capacity value for the 3D interpenetrated structures can reach up to 1792 mA h g-1 at a current density of 50 mA g-1.

11.
Org Lett ; 19(8): 1946-1949, 2017 04 21.
Artigo em Inglês | MEDLINE | ID: mdl-28362099

RESUMO

A palladium-catalyzed two-component coupling of allenylphosphine oxides with conjugated N-tosylhydrazones is revealed. For the first time, the cleavage of α-allenylic aryl ether toward pyrazolemethylene-substituted phosphinyl allenes enabled facile synthesis of combined motifs with pyrazole and allene. Moreover, the obtained adducts could be easily transformed to potential bioactive multifunctionalized phosphinates via a novel alkenyl C-P(O) cleavage.

12.
Ultrason Sonochem ; 27: 287-295, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26186847

RESUMO

In this study, nanoparticles of single-phase akaganeite (ß-FeOOH) were synthesized by an ultrasound-microwave assisted method. The synthesis parameters were optimized by means of response surface methodology. X-ray diffractions (XRD), Fourier transform infrared spectrum (FTIR), UV-vis diffused reflection spectra (UV-vis DRS), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and specific surface area were used to characterize the as-prepared samples. The catalytic activity of the prepared ß-FeOOH was evaluated in a heterogeneous photo-Fenton-like process using methyl orange as target pollutant. It is found that the reaction temperature and interaction between microwave and ultrasound have a significant influence on the catalytic properties of the prepared ß-FeOOH samples. The ß-FeOOH prepared at microwave power of 400 W, ultrasound power of 200 W, reaction temperature of 70°C and reaction time of 3 h, exhibited considerable catalytic activity in weak alkaline solution and under visible light irradiation, which would be of great promise for the industrial application of this catalyst to oxidize organic pollutants for wastewater treatment.

14.
BMC Public Health ; 14: 474, 2014 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-24885822

RESUMO

BACKGROUND: Body mass index (BMI) and hemoglobin (Hb) are positively associated with hypertensive disorders among pregnant women. The aim of this study was to estimate a potential interaction between high BMI and high Hb concentrations on systolic blood pressure (SBP) and diastolic blood pressure (DBP) in pregnancy. METHODS: We recruited 4497 single-birth women aged 18-43 years who received routine antenatal care at three hospitals of Guigang, Guangxi, China, from December 2007 to January 2011. Of 4497 participants, 3472 women were in the first trimester, with following up, 2986 women and 2261 women were left in the second and third trimester, respectively. Clinical data were derived from medical records of each woman. We used multivariable linear regression, by trimesters of pregnancy, to evaluate the associations of high BMI and high Hb concentrations with SBP and DBP according to cross-sectional design. RESULTS: In multivariable analyses, BMI was positively associated with SBP throughout all trimesters, but the corresponding association for Hb concentrations only in the first trimester, whereas both BMI and Hb concentrations were positively associated with DBP in the first and third trimesters. After full adjustment for confounding, the average differences in SBP and DBP comparing women with high BMI and high Hb to those with non-high BMI and non-high Hb were 2.9 mmHg (95% CI: 0.8 to 5.0 mmHg) and 3.9 mmHg (95% CI: 1.5 to 6.3 mmHg) in the first trimester, 2.6 mmHg (95% CI: 0.4 to 4.8 mmHg) and 1.5 mmHg (95% CI: -1.3 to 4.3 mmHg) in the second trimester, and 4.8 mmHg (95% CI: 2.3 to 7.4 mmHg) and 5.7 mmHg (95% CI: 3.2 to 8.3 mmHg) in the third trimester, respectively. With respect to the interaction, significant combined effects between high BMI and high Hb were confirmed on SBP (P = 0.02) and DBP (P = 0.004) in the third trimester, and the amount of interaction on SBP and DBP were 2.0 mmHg (95% CI: 0.1 to 3.9 mmHg) and 2.3 mmHg (95% CI: 0.4 to 4.3 mmHg), respectively. CONCLUSION: Our findings suggest that high BMI and high Hb concentrations may have a synergistic effect on blood pressure in late stage of pregnancy.


Assuntos
Pressão Sanguínea/fisiologia , Índice de Massa Corporal , Hemoglobinas/metabolismo , Hipertensão/etiologia , Obesidade/complicações , Complicações na Gravidez , Adulto , China , Estudos Transversais , Feminino , Humanos , Hipertensão/sangue , Hipertensão/fisiopatologia , Gravidez , Terceiro Trimestre da Gravidez
15.
Pest Manag Sci ; 69(12): 1407-14, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23457050

RESUMO

BACKGROUND: The highly selective auxin-type herbicide quinclorac is widely used to control important dicotyledon and monocotyledon weeds in rice fields. Echinochloa crusgalli var. zelayensis is one of the most troublesome weeds in China, and is very difficult to control in east China due to misuse of herbicides. RESULTS: The JZD -R, JTJ -R, JCW -R and SSX -R biotypes of E. crusgalli var. zelayensis had resistance to quinclorac with resistance levels ranked as JZD -R < JTJ -R < JCW -R < SSX -R. Growth reduction in different biotypes was positively correlated with ethylene production. Stimulated levels of ethylene and 1-aminocyclopropane-1-carboxylic acid (ACC) and activities of ACC synthase and ACC oxidase in resistant biotypes were less than the susceptible biotype, and were negatively correlated with quinclorac resistance levels, suggesting that inhibition of ethylene biosynthesis was positively correlated with resistance levels. CONCLUSION: Considering the resistance-dependent inhibition in the ethylene biosynthetic pathway, the mechanisms of resistance to quinclorac in E. crusgalli var. zelayensis involved alteration(s) in the ethylene response pathway, consisting of at least alteration in induction of the enzymes activity of ACC synthase and ACC oxidase.


Assuntos
Echinochloa/efeitos dos fármacos , Etilenos/biossíntese , Resistência a Herbicidas , Herbicidas/farmacologia , Plantas Daninhas/efeitos dos fármacos , Quinolinas/farmacologia , Aminoácido Oxirredutases/antagonistas & inibidores , Aminoácido Oxirredutases/metabolismo , Vias Biossintéticas/efeitos dos fármacos , Echinochloa/enzimologia , Echinochloa/metabolismo , Liases/antagonistas & inibidores , Liases/metabolismo , Oryza/crescimento & desenvolvimento , Proteínas de Plantas/antagonistas & inibidores , Proteínas de Plantas/metabolismo , Plantas Daninhas/enzimologia , Plantas Daninhas/metabolismo
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