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1.
Food Chem ; 304: 125425, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31476549

RESUMO

Ceramide 2-aminoethylphosphonate (CAEP) is a type of phosphonosphingolipids with potential trophic activity. In this work, complicated CAEP species from different aquatic products were comprehensively identified and semi-quantified by utilizing normal phase liquid chromatography/Q-Exactive mass spectrometry (NPLC/Q-Exactive-MS). We elucidated the fragment schemes of CAEP molecules and found the presence of methylated CAEP (Me-CAEP) species. Remarkably, quantitative results revealed that Loligo chinensis had the highest CAEP content of 4.9 ±â€¯0.4 mg/g dry weight and the most complex molecular species composition, whereas Asterias amurenis had the lowest CAEP content of 1.9 ±â€¯0.6 mg/g dry weight. The most common molecule was CAEP (d19:3-16:0). Additionally, statistical analysis revealed that five aquatic products can be effectively distinguished from their CAEP species; thus, CAEP molecules can play an important role in identifying processed products from aquatic products.

2.
G3 (Bethesda) ; 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31672849

RESUMO

Autophagy is a catabolic process that provides cells with energy and molecular building blocks during nutritional stress. Autophagy also removes misfolded proteins and damaged organelles, a critical mechanism for cellular repair. Earlier work demonstrated that heparan sulfate proteoglycans, an abundant class of carbohydrate-modified proteins found on cell surfaces and in the extracellular matrix, suppress basal levels of autophagy in several cell types during development inDrosophila melanogaster In studies reported here, we examined the capacity of heparan sulfate synthesis to influence events affected by autophagy, including lifespan, resistance to reactive oxygen species (ROS) stress, and accumulation of ubiquitin-modified proteins in the brain. Compromising heparan sulfate synthesis increased autophagy-dependent processes, evident by extended lifespan, increased resistance to ROS, and reduced accumulation of ubiquitin-modified proteins in the brains of ROS exposed adults. The capacity of altering heparan sulfate biosynthesis to protect cells from injury was also evaluated in two different models of neurodegeneration, overexpression of Presenilin andparkin mutants. Presenilin overexpression in the retina produces cell loss, and compromising heparan sulfate biosynthesis rescued retinal patterning and size abnormalities in these animals. parkin is the fly homolog of humanPARK2, one of the genes responsible for juvenile onset Parkinson's Disease. Parkin is involved in mitochondrial surveillance and compromisingparkin function results in degeneration of both flight muscle and dopaminergic neurons inDrosophila Altering heparan sulfate biosynthesis suppressed flight muscle degeneration and mitochondrial dysmorphology, indicating that activation of autophagy-mediated removal of mitochondria (mitophagy) is potentiated in these animals. These findings provide in vivo evidence that altering the levels of heparan sulfate synthesis activates autophagy and can provide protection from a variety of cellular stressors.

3.
Int J Nanomedicine ; 14: 7155-7171, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31564871

RESUMO

Background: Platelet activation and subsequent aggregation are the initial stages of thrombosis. A molecular probe that specifically targets activated platelets and remains retained under high shear stress in vivo can enhance the imaging effect to achieve early and accurate diagnosis. Methods and materials: In this study, we constructed nanoparticles (NPs) using polydopamine to carry two peptides that simultaneously bind integrin αIIbß3 and P-selectin on activated platelets to enhance the targeting of NPs to thrombus. Results: The targeting specificity and binding stability of the NPs on red and white thrombi were demonstrated in vitro using a simulated circulatory device and the targeting effect of the NPs on mixed thrombus was studied by  magnetic resonance (MR)/photoacoustic (PA) dual-modality imaging in vivo. NPs that were surface modified with both peptides have higher selectivity and retention to red and white thrombi in vitro than NPs with a single or no peptide, and the targeting effect was closely related to the number and distribution of activated platelets as well as the structure and type of thrombus. The NPs also have MR/PA dual-modality imaging functionality, significantly enhancing the imaging of mixed thrombus in vivo. Conclusion: These dual-targeted NPs have improved targeting specificity and binding stability to different thrombi under high shear stress and are beneficial for the early diagnosis of thrombosis.

4.
J Agric Food Chem ; 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31657926

RESUMO

EPA-enriched phosphoethanolamine plasmalogens (EPA-pPE), widely present in marine creatures, is a unique glycerophospholipid with EPA at the sn-2 position of glycerol backbone. EPA-pPE has been reported to exhibit numerous distinctive bioactivities. However, the digestion, absorption and metabolism characteristics of EPA-pPE in vivo are not clear, which restrict the molecular mechanism analysis related to its distinctive activities. The aim of the present study was to illustrate the digestion, absorption and metabolism characteristics of EPA-pPE by lipid analysis in serum, intestinal wall and content after oral administration of EPA-pPE emulsion. Results showed the EPA percentage of total fatty acids in serum was increasing over time, with two peaks at 5 and 10 h by 1.89±0.2 and 2.58±0.27, respectively, then fell from 1.89±0.17 at 10h to 1.35±0.16 at16h. In small intestinal content, EPA-pPE was hydrolyzed to lyso-phospholipids and EPA by phospholipases A2, the vinyl ether bond was retained at the sn-1 position. The released EPA could be quickly taken up into the enterocytes and enter circulation. The comparison of simulated digestion in vitro showed that the distinct digestion and absorption process of EPA-pPE was a unique phenomenon. EPA could be retained in serum at high level for a substantial period of time, which suggested us that EPA-pPE was not just a short-lived circulating molecular.

5.
Biosci Biotechnol Biochem ; : 1-13, 2019 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-31608790

RESUMO

Plasmenylethonoamine (pPE) and plasmanylcholine (aPC) are important phospholipid subclasses. Herein we explored optimum conditions for enzymatic purification and preparation of pPE and aPC from the mussel Mytilus edulis and bovine brain. Among them, pPE in Mytilus edulis PE was mainly p18:0-20:5 and p18:0-22:6, and its purity was 92.7%; aPC in PC was primarily a16:0-22:6 and a16:0-20:5, and aPC accounted for 90.2% of PC. We thereafter evaluated neurotrophic effects of Mytilus edulis pPE, aPC, and bovine brain pPE in a NGF-induced PC12 cell model. Morphologically, pPE and aPC could both promote differentiation, manifested in a significant increase in neurite length and number, due to increased expression of synaptophysin and growth protein GAP-43 in a dose-independent and structure-selective manner. Importantly, the effect on neuronal nutrition of pPE was better than aPC, and marine pPE was better than terrestrial pPE, which might be ascribed to vinyl-ether bond and differences in fatty acid composition. Abbreviations: AA: arachidonic acid; DHA: docosahexaenoic acid; EIC: extracted ion chromatogram; EPA: eicosapentanoic acid; GAP: growth-associated protein; HPLC: high-performance liquid chromatography; LC-MS/MS: liquid chromatography-tandem mass spectrometry; LPC: lyso-PC; LPE: lyso-PE; MS: mass spectrometry; NGF: nerve growth factor; PC: phosphatidylcholine; aPC: plasmanylcholine; PE: phosphatidylethanolamine; pPE: plasmenylethonoamine; PG: phosphoglycerols; PLs: phospholipids; PS: phosphoserines; TIC: total ion chromatogram.

6.
Nat Prod Res ; : 1-7, 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31638425

RESUMO

A novel hexanorditerpenoid, hedychin C (1), and a new diterpenoid, hedychin D (2), were isolated from the rhizomes of Hedychium forrestii. Their structures and absolute configurations were unambiguously established by means of extensive spectroscopic data (IR, UV, HRMS, and NMR) and electronic circular dichroism (ECD) calculations. This is the first report of naturally occurring labdane-type hexanorditerpenoid. Compound 1 was proved to have moderate cytotoxicity against XWLC-05 cell line with an IC50 value of 53.6 µM.

7.
Zhongguo Gu Shang ; 32(9): 815-819, 2019 Sep 25.
Artigo em Chinês | MEDLINE | ID: mdl-31615177

RESUMO

OBJECTIVE: To explore the effect of selective lumbosacral posterior rhizotomy(SPR) on lower limb spasticity and gross motor function in patients with cerebral palsy. METHODS: From January 2018 to October 2018, 47 patients with cerebral palsy were treated with rehabilitation alone and SPR combined with rehabilitation. According to whether SPR was performed, the patients were divided into group A and group B. Group A was treated with rehabilitation combined with SPR at lumbosacral level, and group B was treated with rehabilitation alone. There were 23 cases in group A, including 15 males and 8 females, with an average age of (7.30±3.25) years old; 24 cases in group B, 13 males and 11 females, with an average age of (7.00±3.09) years old. Forty-seven patients were assessed with modified Ashworth(MAS) and Gross Motor Function Scale(GMFM-88 items) before and after treatment. The changes of MAS and GMFM-88 scores before and after treatment were compared to evaluate the degree of spasm and the improvement of gross motor function in the two groups. RESULTS: All 47 patients were followed up. At 6 months after treatment, the MAS classification of the two groups was significantly improved(P<0.05), and the improvement of group A was more obvious than that of group B(P<0.05). Six months after treatment, the D, E and total scores of GMFM-88 between two groups were significantly improved compared with those before operation(P<0.05). The improvement of D and total scores in group A was more obvious than that in group B. There was no significant difference in the improvement of area E between two groups. CONCLUSIONS: Selective posterior rhizotomy combined with rehabilitation can significantly improve the spastic state and gross motor function of lower limbs in children with cerebral palsy, and can effectively promote the reconstruction and recovery of motor function of lower limbs in children with cerebral palsy.

8.
ACS Appl Mater Interfaces ; 11(43): 39961-39969, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31580054

RESUMO

Transition-metal phosphides have a potential application in lithium-ion batteries (LIBs) because of their high theoretical capacities and low cost; nevertheless, they possess dramatic volumetric variation during cycling associated with poor conductivity, limiting their practical applications. Here, a three-dimensional (3D) hierarchical flowerlike FeP coated with nitrogen-doped carbon layer (FeP@N,C hybrid) was constructed through a solvothermal method, followed by a phosphating approach under low temperature. N-doped carbon not only suppresses the volume fluctuation of FeP, but also promotes electron transfer, accompanied by catalyzing the decomposition of Li3P to improve the reversibility of the FeP@N,C hybrid during cycling processes. In addition, a 3D flowerlike architecture assembled from porous nanosheets is also beneficial for shortening the migration path of ions as well as improving the contact area of electrode with electrolyte, which enhances the reaction kinetics and is proved by both experimental measurement of Li+ diffusion coefficient and resistivity, along with the calculation of density functional theory. Consequently, the 3D hierarchical flowerlike FeP@N,C hybrid performs excellent cyclic stability (569 mA h g-1 at a current density of 500 mA g-1 for the 300th cycle) and rate performance (331.94 mA h g-1 at a high current density of 5 A g-1) for LIBs. Based on above results, the fabrication strategy in this work could offer a thought to design other high-performance metal phosphide hybrids.

9.
PLoS One ; 14(10): e0222484, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31596855

RESUMO

In nature, protozoa play a major role in controlling bacterial populations. This paper proposes a microfluidic device for the study of protozoa behaviors change due to their chemotactic response in the presence of bacterial cells. A three-channel microfluidic device was designed using a nitrocellulose membrane into which channels were cut using a laser cutter. The membrane was sandwiched between two glass slides; a Euglena suspension was then allowed to flow through the central channel. The two side channels were filled with either, 0.1% peptone as a negative control, or a Listeria suspension respectively. The membrane design prevented direct interaction but allowed Euglena cells to detect Listeria cells as secretions diffused through the nitrocellulose membrane. A significant number of Euglena cells migrated toward the chambers near the bacterial cells, indicating a positive chemotactic response of Euglena toward chemical cues released from Listeria cells. Filtrates collected from Listeria suspension with a series of molecular weight cutoffs (3k, 10k and 100k) were examined in Euglena chemotaxis tests. Euglena cells were attracted to all filtrates collected from the membrane filtration with different molecular weight cutoffs, suggesting small molecules from Listeria might be the chemical cues to attract protozoa. Headspace volatile organic compounds (VOC) released from Listeria were collected, spiked to 0.1% peptone and tested as the chemotactic effectors. It was discovered that the Euglena cells responded quickly to Listeria VOCs including decanal, 3,5- dimethylbenzaldehyde, ethyl acetate, indicating bacterial VOCs were used by Euglena to track the location of bacteria.

10.
Water Res ; 168: 115162, 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31629230

RESUMO

Estimating the proportions of particulate organic carbon (POC) endmembers is essential to fully understand the carbon cycle, the function of aquatic ecosystems, and the migration of contaminants in eutrophic lakes. There is currently no effective remote sensing optical algorithm in the literature to solve this problem. In this study, a POC-source color index (SPOC) was constructed based on the terrestrial and endogenous POC ratios calculated from field-measured stable isotope (δ13CPOC) values. The SPOC algorithm traces the sources of POC by utilizing three spectral bands centered approximately at 560 nm, 674 nm, and 709 nm, covering the intrinsic optical information of different POC sources. At the same time, the SPOC algorithm shows good applicability to Ocean and Land Color Instrument (OLCI), Medium-Resolution Imaging Spectrometer (MERIS), Moderate Resolution Imaging Spectroradiometer (MODIS), and Geostationary Ocean Color Imager (GOCI) image data. The POC sources estimated using the algorithm and monthly OLCI data showed that from March 2018 to January 2019, the POC at the surface of Lake Taihu was mainly terrigenous. In addition, due to multiple factors such as algal blooms, plant physiology, river transport, regional rainfall, and carbon cycling, the distribution of POC sources exhibited strong spatial and temporal heterogeneity. Compared with other methods, it is more convenient to use remote sensing to identify the proportion of POC in different endmembers, which offers a more comprehensive understanding of the energy flows and material circulation in lakes.

11.
Biomed Pharmacother ; 120: 109483, 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31629252

RESUMO

Lung cancer is the leading cause of cancer-related deaths. Ginsenoside Rg3 is the main ingredient of Ginseng which is used to treat non-small cell lung cancer (NSCLC). It has been found to enhance the efficiency of chemotherapy thereby reducing its side effects. Previous studies found that ginsenoside Rg3 can reduce the occurrence of NSCLC by inducing DNA damage. Yet, its anti-DNA damaging effects and mechanisms in tumor cells are still not fully understood. This study explored the effect of ginsenoside Rg3 on DNA repair and VRK1/P53BP1 signaling pathway. Ginsenoside Rg3 treatment significantly decreased the incidence and invasionin a mouse model of lung cancer induced by urethane. The results of cell survival assay and single cell gel electrophoresis showed that ginsenoside Rg3 protected lung adenocarcinoma cells from DNA damage as well as inhibited the proliferation of tumor cells. Ginsenoside Rg3 increased the mRNA and protein expression of VRK1 in NSCLC cells as measured by RT-qPCR and western blot, respectively. These findings suggests that ginsenoside Rg3 regulates VRK1 signaling. Immunofluorescence assays showed that P53BP1 and VRK1 protein level increased, and the VRK1 protein translocated between the nuclei and cytoplasm. Finally, this conclusion was confirmed by the reverse validation in VRK1-knockdown cells. Taken together, these results show that ginsenoside Rg3 upregulate VRK1 expression and P53BP1 foci formation in response to DNA damage thereby inhibiting the tumorigenesis and viability of cancer cells. These findings reveal the role of Rg3 in lung cancer and provides therapeutic targets for developing new drugs in the prevention and treatment of lung cancer.

12.
Comput Methods Programs Biomed ; 183: 105090, 2019 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-31590096

RESUMO

BACKGROUND AND OBJECTIVE: To develop an automatic parapapillary atrophy (PPA) detection algorithm in retinal fundus images and discuss the association between PPA and myopia to facilitate diagnosis and prediction of children myopia. METHODS: The proposed algorithm consists of PPA identification and segmentation, which are evaluated by comparing with ophthalmologist's annotation. The association between PPA parameters and myopia is analyzed via Spearman correlation. RESULTS: The accuracy of PPA identification reaches 90.78%. The F1-score of PPA segmentation is 0.67, and the Pearson correlation between the automatic measurement and ground truths for the area of PPA (APPA), the ratio (µ) of APPA to the area of optic disc (OD) and the maximal width of PPA (W) are 0.74, 0.60, and 0.69 (all p < 0.001). All these parameter changes are significantly correlated with the change of ratio of axial length to corneal curvature (ΔALCC), spherical equivalent (ΔSE), and axial length (ΔAL) (all p < 0.01), in which the highest association is 0.75 between ΔW (the change of W) and ΔALCC. CONCLUSIONS: The proposed algorithm can provide accurate PPA measurement. Strong association between the changes of PPA and the progress of children myopia are observed and the width of PPA has the best association among three PPA parameters.

13.
Chem Commun (Camb) ; 55(76): 11358-11361, 2019 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-31482158

RESUMO

We report on a dual ratiometric two-photon fluorescent probe for in situ sensing of mitochondrial CE activity and pH. Using the probe it is possible to visualize the CE-mediated acidification of hepatoma cells and hepatic tissues during medication with antipyretic anti-inflammatory drugs.


Assuntos
Carboxilesterase/análise , Corantes Fluorescentes/química , Mitocôndrias/metabolismo , Imagem Óptica , Fótons , Anti-Inflamatórios não Esteroides/farmacologia , Antipiréticos/farmacologia , Carboxilesterase/metabolismo , Proliferação de Células/efeitos dos fármacos , Células Hep G2 , Humanos , Concentração de Íons de Hidrogênio , Fígado/efeitos dos fármacos , Fígado/metabolismo , Mitocôndrias/efeitos dos fármacos
14.
Phytochemistry ; 168: 112125, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31541772

RESUMO

Nine undescribed polyacetylated 18-norspirostanol saponins, trilliumosides A‒J, were obtained after a guidance based on a molecular networking strategy from the rhizomes of Trillium tschonoskii. Their structures were established by analysis of comprehensive spectroscopic data and chemical methods after their isolation in pure form. All isolated saponins were evaluated for their cytotoxicities against five selected human cancer cell lines (Huh7,A549,MCF-7,HepG2, and MOLT-4) and anti-inflammatory effects on a lipopolysaccharide (LPS)-stimulated NO production model in RAW264.7 macrophages. Trilliumoside D showed significant cytotoxicity against MOLT-4 cell lines with an IC50 value of 1.0 ±â€¯0.1 µM, whereas trilliumosides H and I displayed remarkable anti-inflammatory effects on NO production with inhibitory rates of 56.3 ±â€¯1.5 and 56.2 ±â€¯2.2% at the concentration of 1.0 µM, respectively.

15.
Theriogenology ; 140: 109-116, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31473493

RESUMO

The study was designed to determine the impact of magnesium (Mg2+) on bovine embryo development. We found that two commercially available sources of bovine serum albumin (BSA) and fetal bovine serum (FBS) contained different amounts of Mg2+ residue: 4 ppm in ICPbio BSA, 114 ppm in Sigma BSA, and 44 ppm in FBS. When CR1 was used as basal medium, PVA and ICPbio BSA produced the lowest blastocyst yield (2.2-2.3%), whereas Sigma BSA increased blastocyst yield to 18.9% (P < 0.05). Supplementation of 1.4 mM MgCl2 into the medium increased the blastocyst rate in the ICPbio BSA group (29.4%) but not in the PVA group (5.4%; P < 0.05) to a level comparable to that of the FBS group (33.7%; P > 0.05). We next found that increasing concentrations of MgCl2 in the culture medium (ICPbio BSA) elevated blastocyst rate from 2.6% (0 mM), 38.4% (0.35 mM) to 50.2% (1.4 mM; P < 0.05), further maintained at 44.9% (2.1 mM) and 43.4% (2.8 mM) (P > 0.05). However, blastocyst rate was reduced to 31.4% (4.2 mM) and 29.4% (5.6 mM) when MgCl2 supplement was increased (P < 0.05). Comparable blastocyst development was achieved in both ICPbio BSA (30.0-33.1%) and Sigma BSA (37.4-38.7%) groups when 1.4 mM Mg2+ was supplemented regardless of its source (MgCl2 vs. MgSO4; P > 0.05). In embryo transfer experiments, higher rates of pregnancy (54.3 vs. 41.5%) and calving (44.3 vs. 32.5%) were achieved in the CR1-Mg2+-supplemented BSA group compared with the FBS group with co-culture, respectively (P < 0.05). These results demonstrate that Mg2+ is a key ion that promotes competent blastocyst and term development. Therefore, a simple and efficient defined medium (CR1-Mg2+-BSA) can successfully replace complex serum and somatic cell co-culture.

16.
Int J Med Inform ; 131: 103954, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31513943

RESUMO

OBJECTIVE: To achieve universal access to medical resources-a partial goal of the second ambitious health reform since 2010-the Chinese government aimed to build a regional medical consortium and enhance the efficiency of health information exchange (HIE). We analyzed the experience of constructing a medical consortium in Chinese hospitals, which was based on regional health information technology (RHIT) promoted by HIE. METHOD: In this longitudinal study, we analyzed the results of the annual surveys that were conducted by the China Hospital Information Management Association from 2006 to 2015. The survey results mainly concerned whether hospitals should join the regional medical consortium, the methods used for sharing inter-hospital medical data, and the out-of-hospital information interaction system. The Bass diffusion model was adopted to fit and predict the proportion of Chinese hospitals joining the consortium from 2006 to 2025. RESULT: As of 2015, the survey results of 7272 hospitals were obtained. The proportion of hospitals in partnership systems increased from 3.0% in 2007 to 57.2% in 2015. There has been a rapid development in the electronic sharing of medical data between hospitals. The proportion of hospitals that relied solely on paper documents for data interaction decreased from 43.3% in 2011 to 8.0% in 2015. There was a strong positive linear correlation between hospitals joining the consortium and the accessibility of electronic medical data exchange within hospitals (r = 0.925). The proportions of hospitals that supported dual referral systems and appointments, data browsing between hospitals and regional information systems, and remote consultation services increased to 65.0%, 61.6%, and 81.9% in 2015, as compared to 18.8%, 16.8%, and 10.9% in 2011, respectively. The Bass prediction model showed that the goal of recruiting 90% of the hospitals to the consortium by 2020 will likely be achieved (adjusted R2 = 0.93). CONCLUSION: The Chinese government has applied a top-down, high-level design model to promote the rapid development of a medical consortium, in which the RHIT technologies are crucial technical enabler.

17.
Exp Gerontol ; : 110737, 2019 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-31521721

RESUMO

INTRODUCTION: Previous studies have stated that cognitive impairment induced by anesthetics was associated with amyloid beta (Aß). However, few researchers have investigated the transport of Aß inside and outside of the brain. AIM: We attempted to probe the effects of sevoflurane on cognitive functions, the plasma Aß, and transporters of Aß in aged mice. The receptor for advanced glycation end-products (RAGE) is an Aß influx protein, and Low-density lipoprotein receptor-related protein-1 (LRP-1) is an Aß efflux protein. METHODS: Aged mice were divided into the control group and the sevoflurane group. The mice were exposed to 100% oxygen or 2.5% sevoflurane for 2 h. The abilities of spatial learning and memory in mice were tested using the Morris water maze. Aß concentrations of plasma were measured with enzyme-linked immunosorbent assay kits. The RAGE and LRP-1 gene levels in the brain were assessed with quantitative polymerase chain reaction, and the protein levels were determined by western blot analysis. The locations of RAGE in the brain were confirmed via immunofluorescence. RESULTS: In the sevoflurane group mice, the escape latency was increased on the 5th day of training, and the time spent in the target quadrant was decreased on the 7th day after anesthesia. Sevoflurane reduced the concentration of plasma Aß1-40. In addition, sevoflurane increased both gene and protein levels of RAGE in the brain, and increased RAGE proteins co-localized with the hippocampal vascular endothelial cells. CONCLUSION: RAGE over-expression in the hippocampal vascular endothelial cells possibly resulted in the excessive transport of the plasma Aß1-40 into the brain after treatment with sevoflurane, which was associated with sevoflurane-induced cognitive dysfunction in aged mice.

19.
Chem Biodivers ; 2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31487435

RESUMO

Phytochemical study on the fruit of Cornus officinalis Sieb. et Zucc. yielded two new iridoid glucosides, named cornusglucoside A (1) and cornusglucoside B (2). The structures of 1 and 2 were elucidated via comprehensive NMR and HR-ESI-MS data analysis. Additionally, their inhibitory effects on IL-6-induced STAT3 activation were assessed.

20.
Adv Mater ; 31(42): e1903912, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31489716

RESUMO

Molecular additives are often used to enhance dynamic motion of polymeric chains, which subsequently alter the functional and physical properties of polymers. However, controlling the chain dynamics of semiconducting polymer thin films and understanding the fundamental mechanisms of such changes is a new area of research. Here, cycloparaphenylenes (CPPs) are used as conjugated molecular additives to tune the dynamic behaviors of diketopyrrolopyrrole-based (DPP-based) semiconducting polymers. It is observed that the addition of CPPs results in significant improvement in the stretchability of the DPP-based polymers without adversely affecting their mobility, which arises from the enhanced polymer dynamic motion and reduced long-range crystalline order. The polymer films retain their fiber-like morphology and short-range ordered aggregates, which leads to high mobility. Fully stretchable transistors are subsequently fabricated using CPP/semiconductor composites as active layers. These composites are observed to maintain high mobilities when strained and after repeated applied strains. Interestingly, CPPs are also observed to improve the contact resistance and charge transport of the fully stretchable transistors. ln summary, these results collectively indicate that controlling the dynamic motion of polymer semiconductors is proved to be an effective way to improve their stretchability.

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