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1.
Antiviral Res ; 195: 105193, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34687820

RESUMO

Transient receptor potential mucolipin 2 and 3 (TRPML2 and TRPML3), as key channels in the endosomal-lysosomal system, are associated with many different cellular processes, including ion release, membrane trafficking and autophagy. In particular, they can also facilitate viral entry into host cells and enhance viral infection. We previously identified that two selective TRPML agonists, ML-SA1 and SN-2, that showed antiviral activities against dengue virus type 2 (DENV2) and Zika virus (ZIKV) in vitro, but their antiviral mechanisms are still elusive. Here, we reported that ML-SA1 could inhibit DENV2 replication by downregulating the expression of both TRPML2 and TRPML3, while the other TRPML activator, SN-2, suppressed DENV2 infection by reducing only TRPML3 expression. Consistently, the channel activities of both TRPML2 and TRPML3 were also found to be associated with the antiviral activity of ML-SA1 on DENV2 and ZIKV, but SN-2 relied only on TRPML3 channel activity. Further mechanistic experiments revealed that ML-SA1 and SN-2 decreased the expression of the late endosomal marker Rab7, dependent on TRPML2 and TRPML3, indicating that these two compounds likely inhibit viral infection by promoting vesicular trafficking from late endosomes to lysosomes and then accelerating lysosomal degradation of the virus. As expected, neither ML-SA1 nor SN-2 inhibited herpes simplex virus type I (HSV-1), whose entry is independent of the endolysosomal network. Together, our work reveals the antiviral mechanisms of ML-SA1 and SN-2 in targeting TRPML channels, possibly leading to the discovery of new drug candidates to inhibit endocytosed viruses.

3.
Artigo em Inglês | MEDLINE | ID: mdl-34431054

RESUMO

Biostimulant application is an effective strategy to enhance soil fertility and plant growth. However, its comprehensive impacts on nitrogen (N) uptake and reactive N (Nr) losses via leaching, ammonia (NH3) volatilization, and nitrous oxide (N2O) emission from plastic-shed greenhouse vegetable system are still little known. Therefore, a field experiment was conducted with cauliflower-tomato growth rotation (from September 6, 2018, to July 17, 2019) receiving three biostimulants, i.e., humic acid (HA), algae extract (AE), and chitosan (CT), as well as a control without stimulant. The cumulative Nr losses over the cauliflower-tomato growth cycle via leaching, NH3 volatilization, and N2O emission were 104-175 kg N ha-1, 2.32-3.85 kg N ha-1, and 0.70-0.85 kg N ha-1, respectively. Biostimulant application significantly (P < 0.05) retarded the total N leaching by 17-44% in tomato season, while suppressed the NH3 volatilization by 18-38% in cauliflower season. Overall, AE showed the best inhibition efficiency on Nr losses by significantly (P < 0.05) decreasing total N leaching and NH3 volatilization by 36-44% and 38-52% in both vegetable seasons, compare to the control. However, all three biostimulants stimulated the N2O emission under both vegetable cycles. Interestingly, all biostimulant-added treatments promote the cauliflower and tomato yield, particularly following the HA and AE amendments, which bring local farmers approximately 4,384-10,035 yuan RMB ha-1 more income. Enhanced yield under biostimulant treatments was due to higher N uptake capacity and enhanced root morphology. In summary, biostimulants have a contrasting influence on three major Nr lost pathways in greenhouse vegetable production. We recommend that AE is the most optimal biostimulant as it increases vegetable yield and decreases total N leaching and NH3 volatilization while not dramatically increase the N2O emission.

4.
Int J Biol Sci ; 17(9): 2348-2355, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34239361

RESUMO

Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has led to more than 150 million infections and about 3.1 million deaths up to date. Currently, drugs screened are urgently aiming to block the infection of SARS-CoV-2. Here, we explored the interaction networks of kinase and COVID-19 crosstalk, and identified phosphoinositide 3-kinase (PI3K)/AKT pathway as the most important kinase signal pathway involving COVID-19. Further, we found a PI3K/AKT signal pathway inhibitor capivasertib restricted the entry of SARS-CoV-2 into cells under non-cytotoxic concentrations. Lastly, the signal axis PI3K/AKT/FYVE finger-containing phosphoinositide kinase (PIKfyve)/PtdIns(3,5)P2 was revealed to play a key role during the cellular entry of viruses including SARS-CoV-2, possibly providing potential antiviral targets. Altogether, our study suggests that the PI3K/AKT kinase inhibitor drugs may be a promising anti-SARS-CoV-2 strategy for clinical application, especially for managing cancer patients with COVID-19 in the pandemic era.


Assuntos
COVID-19/tratamento farmacológico , Proteínas Proto-Oncogênicas c-akt/antagonistas & inibidores , Pirimidinas/uso terapêutico , Pirróis/uso terapêutico , SARS-CoV-2/efeitos dos fármacos , Internalização do Vírus/efeitos dos fármacos , Animais , COVID-19/enzimologia , Chlorocebus aethiops , Simulação por Computador , Humanos , Neoplasias/enzimologia , Neoplasias/mortalidade , Proteínas Proto-Oncogênicas c-akt/metabolismo , Pirimidinas/farmacologia , Pirróis/farmacologia , Receptor Cross-Talk , Células Vero
5.
Sci Rep ; 10(1): 17975, 2020 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-33087812

RESUMO

The high coercivity of Nd-Fe-B magnets can also be obtained in the Ce-Fe-B magnets fabricated via the dual-main-phase (DMP) method in which the high abundance Ce was used to substitute Nd(Pr). The inhomogeneous distributions of the matrix grains in the DMP magnet play a key role in the enhanced magnetic performance. Compared with the single-phase magnet, more grain boundary phases encapsulating the matrix 2:14:1 grain are formed in the DMP magnet, which reduce the exchange coupling between adjacent magnetic grains. The switching field distribution and the interaction field distribution of the Ce-Fe-B magnets were determined by the first-order-reversal curves (FORC). The switching field peaks around 6 kOe, 11 kOe and 12 kOe in the FORC distribution indicate that three major reversal components coexist for the DMP magnet. The overlapp of the second and third switching field peaks reveals the presence of a pinning interaction within individual magnetic grains with a core-shell structure, which further improve the coercivity of the magnet.

6.
Carbohydr Polym ; 224: 115164, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31472866

RESUMO

In this work, the properties of the plasticized hemicelluloses/chitosan-based edible films reinforced by cellulose nanofiber (CNF) have been evaluated. Results showed that the tensile strength (TS) of the film increased by 2.3 times with adding 5% CNF. As compared with unplasticized film, the films containing 10-40% plasticizers (glycerol, xylitol, sorbitol) showed slightly higher water vapor permeability, oxygen permeability, transparency, solubility, and lower contact angle. Among the three types of plasticized films, glycerol-plasticized films exhibited the highest tensile strain at break (TB, 7.80-18.53%), while the sorbitol-plasticized films exhibited the highest TS (23.14-48.96 MPa). However, there were no substantial differences in the three types of plasticized films except TS and TB. Overall, the films containing 20-30% glycerol with high TS (31.02-38.56 MPa) and TB (10.07-15.98%) were suitable for food-packaging applications where high mechanical strength and flexibility are required.

7.
Food Chem ; 298: 125041, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31261000

RESUMO

Edible packaging films play an important role in extending the shelf life of food products. In this work, the properties of cellulose nanofiber (CNF) reinforced hemicelluloses/chitosan-based edible films with xylooligosaccharides (XOS) have been evaluated. Results showed that the tensile strength (TS) of the film can be increased by 2.5 times with adding 5 wt% CNF. Incorporating 1.79-7.18% XOS into hemicelluloses-chitosan matrix only caused slightly higher water vapor permeability, and the composite films exhibited good hydrophobicity, thermal stability, and high transparency. The hemicelluloses/chitosan films with 1.79-5.38% XOS had higher TS (42.7-50.7 MPa) and lower oxygen permeability (OP, 4.95-5.06 cm3 µm/m2·day·kPa) than those containing 7.18% XOS. Additionally, ∼92.6% XOS in films can be released in simulated gastric fluid within 60 min. Overall, XOS (1.79-5.38%) with prebiotic properties can be added to films successfully to improve the functionality and the films were fit for food-packaging where high TS and low OP are required.


Assuntos
Celulose/química , Quitosana/química , Glucuronatos/química , Nanofibras/química , Oligossacarídeos/química , Embalagem de Alimentos/métodos , Suco Gástrico , Humanos , Microscopia Eletrônica de Varredura , Permeabilidade , Polissacarídeos/química , Solubilidade , Espectroscopia de Infravermelho com Transformada de Fourier , Vapor , Resistência à Tração , Difração de Raios X
8.
Carbohydr Polym ; 195: 114-119, 2018 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-29804958

RESUMO

Effect of sonication on the extractability and physico-chemical properties of hemicelluloses from Eucalyptus grandis using 5% KOH solution at 50 °C for 3 h has been comparatively studied. The results showed that the yield of hemicelluloses increased from 2.6 to 19.6% as the ultrasonic time was extended from 5 to 35 min. The highest yield of hemicelluloses (95.2%) was achieved at 30 min ultrasonic time. Xylose was the dominant sugar (82.94-84.96%) of all the hemicellulosic fractions. Furthermore, the hemicelluloses obtained by ultrasound-assisted extractions had slightly lower molecular weights (74,510-66,770 g/mol) and thermal stabilities, but higher contents of xylose (83.95-84.96%). The increased yield of ultrasonically extracted hemicelluloses, which have preserved their main structural properties, confirmed the great potential of ultrasound-assisted extraction to separate hemicelluloses from Eucalyptus grandis at an industrial level.

9.
Exp Ther Med ; 14(3): 1941-1946, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28962107

RESUMO

Airway pressure release ventilation (APRV) is a ventilator mode which has demonstrated potential benefits in acute respiratory distress syndrome (ARDS) patients. We therefore sought to compare relevant pulmonary data and safety outcomes of this mode to the conventional ventilation and sustained inflation. Canines admitted after intravenous injection of oleic acid requiring mechanical ventilation were randomly divided into 3 groups (n=6), namely conventional ventilation group, low tidal volume ventilation with recruitment group (LTV+SI) and APRV group. The changes of oxygenation, ventilation, airway pressure, inflammatory reaction and hemodynamics at the basic state were observed at 0, 1, 2 and 4 h during the experiment. The levels of PaO2/FiO2 in APRV group were higher than LTV+SI group at 2 and 4 h (P<0.05). In APRV group, the PCO2 levels at 1, 2 and 4 h is much lower than LTV+SI group (P<0.05). Outcome variables showed no differences between APRV, LVT+SI and conventional mechanical ventilation for plateau airway pressure (24±1 vs. 29±3 vs. 25±4), mean arterial pressure (92.9±16.5 vs. 85.8±21.4 vs. 88.7±24.4), cardiac index (4.3±1.7 vs. 3.5±1.9 vs. 3.4±2.1), ERO2 (13.4±10.3 vs. 16.1±6.8 vs. 17.6±9.1), lac (2.5±1.7 vs. 3.1±1.6 vs. 3.9±1.9), tumor necrosis factor (TNF)-α (132±11 vs. 140±6 vs. 195±13) and matrix metalloproteinase (MMP)-9. For canines sustaining acute respiratory distress syndrome requiring mechanical ventilation, APRV can significantly improve oxygenation and keep hemodynamic stability compared with LTV+SI. The results of TNF-α and MMP-9 suggest that APRV could be as protective for ARDS as LTV with recruitment group.

10.
BMC Plant Biol ; 16(1): 116, 2016 05 23.
Artigo em Inglês | MEDLINE | ID: mdl-27215938

RESUMO

BACKGROUND: Drought stress is one of the major causes of crop loss. WRKY transcription factors, as one of the largest transcription factor families, play important roles in regulation of many plant processes, including drought stress response. However, far less information is available on drought-responsive WRKY genes in wheat (Triticum aestivum L.), one of the three staple food crops. RESULTS: Forty eight putative drought-induced WRKY genes were identified from a comparison between de novo transcriptome sequencing data of wheat without or with drought treatment. TaWRKY1 and TaWRKY33 from WRKY Groups III and II, respectively, were selected for further investigation. Subcellular localization assays revealed that TaWRKY1 and TaWRKY33 were localized in the nuclei in wheat mesophyll protoplasts. Various abiotic stress-related cis-acting elements were observed in the promoters of TaWRKY1 and TaWRKY33. Quantitative real-time PCR (qRT-PCR) analysis showed that TaWRKY1 was slightly up-regulated by high-temperature and abscisic acid (ABA), and down-regulated by low-temperature. TaWRKY33 was involved in high responses to high-temperature, low-temperature, ABA and jasmonic acid methylester (MeJA). Overexpression of TaWRKY1 and TaWRKY33 activated several stress-related downstream genes, increased germination rates, and promoted root growth in Arabidopsis under various stresses. TaWRKY33 transgenic Arabidopsis lines showed lower rates of water loss than TaWRKY1 transgenic Arabidopsis lines and wild type plants during dehydration. Most importantly, TaWRKY33 transgenic lines exhibited enhanced tolerance to heat stress. CONCLUSIONS: The functional roles highlight the importance of WRKYs in stress response.


Assuntos
Arabidopsis/fisiologia , Secas , Regulação da Expressão Gênica de Plantas , Temperatura Alta , Plantas Geneticamente Modificadas/fisiologia , Fatores de Transcrição , Triticum/genética , Adaptação Fisiológica/genética , Arabidopsis/genética , Filogenia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas/genética , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo
11.
Exp Ther Med ; 12(6): 3873-3876, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28105119

RESUMO

The clinical efficacy and safety of different combinations of non-bio artificial liver in the treatment of acute liver failure was examined. A total of 61 cases were selected under blood purification treatment from the patients with severe acute liver failure admitted to the severe disease department of the hospital from December, 2010 to December, 2015. Three types of artificial liver combinations were observed, i.e., plasma exchange plus hemoperfusion plus continuous venovenous hemodiafiltration (PE+HP+CVVHDF), PE+CVVHDF and HP+CVVHDF. The heart rate (HR), mean arterial pressure (MAP), respiratory index (PaO2/FiO2), liver and kidney function indicator, as well as platelet and coagulation function were compared. A comparison before and after the treatment using the three methods, showed improvement in the HRs, MAPs, PaO2/FiO2, total bilirubins (TBIL) and alanine aminotransferases (ALT) (P<0.05), of which TBIL and ALT were decreased more significantly (P<0.01) in the PE+CVVHDF and PE+HP+CVVHDF groups. Only changes in the PE+HP+CVVHDF and PE+CVVHDF groups were statistically significant after prothrombin time and albumin treatment (P<0.05). The difference between the decrease in TBIL in the PE+HP+CVVHDF group and that in the HP+CVVHDF group was statistically significant (P<0.05). Treatment of the 61 patients using the artificial liver support system yielded a survival rate of 62.3% (38/61), and a viral survival rate of 35.0% (7/20); with the non-viral survival rate being 75.6% (31/41). In conclusion, following the treatment of three types of artificial livers, the function was improved to varying degrees, with the PE+HP+CVVHDF and the PE+CVVHDF method being better. By contrast, after the treatment of non-viral liver failure, the survival rate was significantly higher than the patients with viral liver failure.

12.
Cell Biochem Biophys ; 72(3): 807-12, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25680826

RESUMO

Shenfu injection (SFI) derived from traditional Chinese medicine has been widely used in cardiovascular diseases. The objective of this study was to determine the effect of SFI and conventional early goal-directed therapy (EGDT) on organ functions and outcomes of septic shock patients. For this purpose, a total of 45 septic shock patients were randomly divided into control group A (24 patients on EGDT) and experimental group B (21 patients on SFI + EGDT). SFI was administered (100@20 mL/h) twice daily. Hemodynamic status, lactic acid, and vasoactive drug use were observed before and after treatment. Other indicators included ventilator weaning time, ICU stay time, free of organ failure time, and 28-day hospital mortality. Regarding experimental group, compared with controls, BUN/creatinine decreased significantly at 3, 5, and 7 days while PaO2/FiO2 increased at 1 and 3 days (P < 0.05). APACHE-II and SOFA scores decreased in both groups at 3, 5, and 7 days (P < 0.05), whereas SOFA scores improved more in experimental group as compared with controls. Ventilator weaning time and ICU stay were significantly shorter in experimental group as compared with controls. In both groups, mean arterial pressure/systemic vascular resistance index post-treatment levels increased and lactic acid decreased at 6, 12, 24, 48, and 72 h (P < 0.05). Heart rate decreased at 24, 48, and 72 h (P < 0.05); while gamma-glutamyl transpeptidase and glutamate oxaloacetate transaminase levels increased at 1 day and 1 and 3 days, respectively (P < 0.05). Combined use of SFI and EGDT can improve hemodynamics, reduce the damage to vital organs, and shorten ventilation and ICU stay times in septic shock patients.


Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Choque Séptico/tratamento farmacológico , Adulto , Idoso , Idoso de 80 Anos ou mais , Medicamentos de Ervas Chinesas/administração & dosagem , Feminino , Hemodinâmica , Humanos , Injeções , Ácido Láctico/sangue , Masculino , Pessoa de Meia-Idade , Escores de Disfunção Orgânica , Transaminases/sangue , Desmame do Respirador
13.
World J Emerg Med ; 5(3): 214-7, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25225587

RESUMO

BACKGROUND: Acute liver failure (ALF) caused by viral and non-viral hepatitis is often accompanied with severe metabolic disorders, the accumulation of toxic substances and continuous release and accumulation of a large number of endogenous toxins and inflammatory mediators. The present study aimed to investigate the effects of various combined non-biological artificial liver treatments for patients with acute liver failure (ALF) complicated by multiple organ dysfunction syndrome (MODS). METHODS: Thirty-one patients with mid- or late-stage liver failure complicated by MODS (score 4) were randomly divided into three treatment groups: plasmapheresis (PE) combined with hemoperfusion (HP) and continuous venovenous hemodiafiltration (CVVHDF), PE+CVVHDF, and HP+CVVHDF, respectively. Heart rate (HR) before and after treatment, mean arterial pressure (MAP), respiratory index (PaO2/FiO2), hepatic function, platelet count, and blood coagulation were determined. RESULTS: Significant improvement was observed in HR, MAP, PaO2/FiO2, total bilirubin (TBIL) and alanine aminotransferase (ALT) levels after treatment (P<0.05). TBIL and ALT decreased more significantly after treatment in the PE+CVVHDF and PE+HP+CVVHDF groups (P<0.01). Prothrombin time (PT) and albumin were significantly improved only in the PE+CVVHDF and PE+HP+CVVHDF groups (P<0.05). TBIL decreased more significantly in the PE+HP+CVVHDF group than in the HP+CVVHDF and PE+CVVHDF groups (P<0.05). The survival rate of the patients was 58.1% (18/31), viral survival rate 36.4% (4/11), and non-viral survival rate 70% (14/20). CONCLUSION: Liver function was relatively improved after treatment, but PE+HP+CVVHDF was more efficient for the removal of toxic metabolites, especially bilirubin. The survival rate was significantly higher in the patients with non-viral liver failure than in those with viral liver failure.

14.
Cell Biochem Biophys ; 69(3): 699-702, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24671670

RESUMO

The aim of this study is to investigate whether hemodiafiltration combined with resin-mediated absorption is a better therapy for hyperlipidemic acute pancreatitis. Patients (n = 67) with acute pancreatitis treated in ICU from January 2009 to December 2012 were included in this study. Seven of these 67 cases were diagnosed hyperlipidemic acute pancreatitis (HLAP). All the 7 HLAP patients went through fast, gastrointestinal decompression, anti-shock treatment, inhibition of pancreatic secretion, antiseptic treatments, and hemoperfusion (HP) combined with continuous veno venous hemodiafiltration (CVVHDF). After one round of treatment by resin adsorption, there was a significant decrease in serum triglycerides (TG) (29.78 %) and total cholesterol (TC) (24.02 %) levels (p < 0.01). TG and TC levels dropped by 49.02 and 37.66 %, respectively, after 1-day treatment of HP + CVVHDF; by 62.81 and 47.37 % on day 2 post-treatment; and by 69.57 and 49.47 % on day 3 post-treatment. All the 7 patients survived. The average time spent in the ICU was 7 ± 3.8 days, and the average duration of hospitalization was 19 ± 15.1 days. Our results show that hemoperfusion combined with hemodiafiltration is an efficient treatment as this approach can reduce plasma lipid levels effectively and reduce the risk of acute pancreatitis due to hyperlipidemia.


Assuntos
Hemodiafiltração , Hiperlipidemias/complicações , Pancreatite/complicações , Pancreatite/terapia , Resinas Sintéticas/química , Absorção Fisico-Química , Doença Aguda , Adulto , Terapia Combinada , Feminino , Humanos , Pessoa de Meia-Idade , Pancreatite/metabolismo
15.
Cell Biochem Biophys ; 68(3): 571-5, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24006155

RESUMO

We sought to study the clinical efficacy of various combined blood purification techniques in patients with non-viral acute liver failure complicated by multiple organ dysfunction syndrome (MODS). For this purpose, 19 patients diagnosed of mid- or late-stage liver failure with MODS score-4 were randomly divided into 3 treatment groups of PE+HP+CVVHDF, PE+CVVHDF, and HP+CVVHDF, respectively. Pre- and post-treatment heart rate (HR), mean arterial pressure (MAP), arterial blood gases (pH, PaO2, and PaCO2), hepatic function, platelet count, and blood coagulation were determined. The data show significant improvement in HR, MAP, PaO2/FiO2, total bilirubin (TBIL), and alanine aminotransferase (ALT) levels after treatment (P < 0.05). TBIL decreased more significantly after treatment in PE+CVVHDF and PE+HP+CVVHDF groups (P < 0.01). Significant improvement in prothrombin time and albumin was observed only in PE+CVVHDF and PE+HP+CVVHDF groups (P < 0.05). The decrease of TBIL and improvement of PaO2/FiO2 ratio were more pronounced in PE+HP+CVVHDF than in HP+CVVHDF group (P < 0.05). To conclude, liver function was relatively improved by all the three combined blood purification techniques used; however, PE+HP+CVVHDF approach was found more efficient in the removal of toxic metabolites, especially bilirubin. The data suggest that the combined blood purification techniques used were effective and involved minor side effects.


Assuntos
Hemodiafiltração , Hemoperfusão , Falência Hepática Aguda/terapia , Troca Plasmática , Adulto , Idoso , Idoso de 80 Anos ou mais , Coagulação Sanguínea , Terapia Combinada/economia , Análise Custo-Benefício , Feminino , Hemodinâmica , Humanos , Fígado/fisiopatologia , Falência Hepática Aguda/sangue , Falência Hepática Aguda/fisiopatologia , Masculino , Pessoa de Meia-Idade , Oxigênio/sangue , Contagem de Plaquetas , Adulto Jovem
16.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue ; 20(8): 449-51, 2008 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-18687168

RESUMO

OBJECTIVE: To observe the effects of sedation with midazolam and propofol on anterograde amnesia in critical patients. METHODS: Sixty selected patients on mechanical ventilation in intensive care unit (ICU) were randomly divided into three subgroups (propofol, midazolam, and midazolam and propofol combination group), with 20 cases in each group. Patients who were awakened from sedation were showed with a card depicted with different colors, figures and numbers. When patients were totally conscious after weaning from mechanical ventilation,the influence of the different methods of sedation on anterograde amnesia of these critically ill patients was assessed. RESULTS: (1) 70%, 95% and 90% of patients manifested amnesia in propofol, midazolam and the combination group, respectively. All the patients recovered their memory immediately in 30 minutes after withdrawal of the sedatives. (2) When midazolam was compared with propofol and combination group, time of onset was obviously prolonged after an intravenous injection of a load dose in midazolam group [(2.7+/-1.1) minutes and (3.1+/-1.3) minutes vs. (5.1+/-2.8) minutes], also was time of extubation after regaining of consciousness [(0.7+/-0.2) hour and (1.2+/-0.6) hours vs. (2.7+/-0.3) hours, all P<0.01]. There was no significant difference between propofol group and the combination group in time of onset and extubation (both P>0.05). (3) Cost of propofol [(2,100+/-125) yuan] was 75% higher than that of midazolam [(1,200+/-112) yuan, P<0.01], but cost of sedatives in the combination group [(1,300+/-132) yuan] was similar to that in midazolam group (P>0.05). CONCLUSION: Combination of midazolam and propofol can not only ensure anterograde amnesia in critical patients, reduce drug dosage and adverse reactions, but also can help reduce the hospital expenses. This method may be a better sedation program in ICU.


Assuntos
Amnésia/induzido quimicamente , Hipnóticos e Sedativos/administração & dosagem , Midazolam/administração & dosagem , Propofol/administração & dosagem , Adolescente , Adulto , Estado Terminal , Quimioterapia Combinada , Feminino , Humanos , Unidades de Terapia Intensiva , Masculino , Pessoa de Meia-Idade , Respiração Artificial , Adulto Jovem
17.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue ; 18(6): 355-8, 2006 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-16784564

RESUMO

OBJECTIVE: To study the effects of lung recruitment maneuvers (RM) in acute respiratory distress syndrome (ARDS) with pulmonary and extrapulmonary origin (ARDSp/ARDSexp). METHODS: Thirty-seven ARDS patients with a forced expiratory volume were selected (16 with ARDSp, 21 with ARDSexp). Without using any sedative and neuromuscular blocking agents, with continuous positive airway pressure (CPAP) up to 30 cm H(2)O (1 cm H(2)O=0.098 kPa), the patients were asked to hold the breath for 30 seconds, and then CPAP was lowered to the level of that before the treatment within 5-10 seconds. RESULTS: Compared with the state before recruitment, changes in heart rate (HR) were not obvious in two groups during RM, mean arterial pressure was elevated evidently after 2 minutes in two groups (both P<0.05), and central venous pressure (CVP) rose after 30 seconds in two groups (both P<0.05). Peak inspiratory pressure, platform pressure and mean airway pressure increased clearly after 30 seconds (all P<0.05), and pulmonary compliance decreased obviously 30 seconds after re-inflation recruitment in two groups (both P<0.05), and increased obviously in ARDSexp group 5, 15, 30 minutes after recruitment (all P<0.05). In the group with ARDSp oxygenation index (OI) changed distinctly 10, 30 minutes and 1 hour after recruitment compared with the state before recruitment (all P<0.05). In patients with ARDSexp OI was significantly improved 10 minutes, 30 minutes, 1 hour, 2 hours after RM. CONCLUSION: In ARDSexp, with pulmonary interstitial edema as the main pathology, responded better to RM than ARDSp with pulmonary consolidation.


Assuntos
Pressão Positiva Contínua nas Vias Aéreas , Síndrome do Desconforto Respiratório/fisiopatologia , Adulto , Idoso , Gasometria , Pressão Sanguínea , Pressão Venosa Central , Feminino , Frequência Cardíaca , Humanos , Complacência Pulmonar , Masculino , Pessoa de Meia-Idade , Síndrome do Desconforto Respiratório/terapia , Resultado do Tratamento
18.
Artigo em Chinês | MEDLINE | ID: mdl-16464390

RESUMO

OBJECTIVE: To investigate the effects of low-dose dopamine and dobutamine on renal functioning patients with nonoliguric renal failure. METHODS: Twenty-nine hemodynamically stable patients with nonoliguric renal failure were enrolled to this study group. Each patient was given 5% glucose (control), dopamine or dobutamine in random order by means of an injection pump every 4 hours. The change in urine output, fractional excretion of sodium (FeNa) and creatinine clearance rate (CCr) were determined. RESULTS: Compared with control, urine volume, FeNa, and CCr were increased significantly after administration of dopamine, or dobutamine. Compared with dobutamine, dopamine could increase the urine output and the FeNa markedly, but there was no difference in CCr increase between dopamine and dobutamine. CONCLUSION: Dopamine or dobutamine treatment could significantly increase the urine output, FeNa, and CCr in patients with nonoliguric renal failure. Dopamine infusion markedly increases urine output and the FeNa, but there is no significantly difference in CCr between dopamine and dobutamine treatment.


Assuntos
Dobutamina/administração & dosagem , Dopamina/administração & dosagem , Insuficiência Renal/tratamento farmacológico , Adulto , Idoso , Feminino , Humanos , Rim/efeitos dos fármacos , Rim/fisiopatologia , Masculino , Pessoa de Meia-Idade , Insuficiência Renal/fisiopatologia , Adulto Jovem
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