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2.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 41(3): 430-434, 2019 Jun 30.
Artigo em Chinês | MEDLINE | ID: mdl-31282342

RESUMO

Bladder cancer is a urological malignant tumor with high morbidity and mortality. Masses protruding into the bladder cavity is an important feature for clinical diagnosis of bladder cancer. However,patients with encrusted bladder cancer(EBC)do not present with masses protruding into the bladder cavity and thus this malignancy is often misdiagnosed. Four patients were admitted in Peking University People's Hospital from July 2015 to February 2017. All of them were males aged 40 to 77 years(mean:58 years). Patients were mainly manifested as frequent urination,urgency,nocturia,and decreased bladder capacity,with or without difficulty of voiding.Although the bladder walls were markedly thickened,there was no obvious mass on imaging scans. Three patients received urodynamic test,which showed the maximum capacity of the bladder was 41 to 128 ml(mean:91 ml). One patient presented with gross hematuria,two patients presented with microscopic hematuria,and the remaining one patient had no hematuria. No mass was observed by cystoscopy. All of the patients were diagnosed with bladder cancer by repeated biopsy or intraoperative frozen section analysis.


Assuntos
Neoplasias da Bexiga Urinária/diagnóstico , Neoplasias da Bexiga Urinária/patologia , Adulto , Idoso , Biópsia , Cistoscopia , Hematúria , Humanos , Masculino , Pessoa de Meia-Idade
3.
Talanta ; 200: 494-502, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31036214

RESUMO

An effective dithiourea-appended 1,8-naphthalimide fluorescent probe was designed and synthesized. This probe could recognize Hg2+ and Ag+ sensitively and selectively in neutral and alkaline conditions. Moreover, the probe detected Hg2+ alone at pH between 2 and 6. The sensing ability of the probe was explored by UV-vis, fluorescence, FTIR and 1H NMR spectroscopy. The probe was quenched by Hg2+ and Ag+ with 1:1 binding ratios in MeCN/H2O (4/1, v/v) mixed solution with binding constants of 3.76 × 104 L mol-1 and 2.47 × 104 L mol-1, respectively. The linear concentration ranges for Hg2+ and Ag+ were 0-17 µmol L-1 and 0-24 µmol L-1 with detection limits of 0.83 µmol L-1 and 1.20 µmol L-1, respectively, which allowed for the quantitative determination of Hg2+ and Ag+. The new probe, 3a, was successfully applied to the fluorescence imaging of Hg2+ and Ag+ in HepG2 cells, demonstrating its potential application in biological science. Moreover, 3a was used to measure Hg2+ and Ag+ in tap water, drinking water and ultrapure water samples. The recoveries of Hg2+ and Ag+ in water samples were 96-99% and 98-103%, respectively. Therefore, the proposed method showed promising perspectives for its application, aimed at detecting Hg2+ and Ag+ in fluorescence imaging and real water samples.


Assuntos
Corantes Fluorescentes/química , Mercúrio/análise , Naftalimidas/química , Imagem Óptica , Prata/análise , Tioureia/química , Corantes Fluorescentes/síntese química , Células Hep G2 , Humanos , Imagem Molecular , Estrutura Molecular , Células Tumorais Cultivadas
4.
J Agric Food Chem ; 65(30): 6274-6281, 2017 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-28691485

RESUMO

Better milk safety control can offer important means to promote public health. However, few technologies can detect different types of contaminants in milk simultaneously. In this regard, the present work proposes a single-drop Raman imaging (SDRI) strategy for semiquantitation of multiple hazardous factors in milk solutions. By developing SDRI strategy that incorporates the coffee-ring effect (a natural phenomenon often presents in a condensed circle pattern after a drop evaporated) for sample pretreatment and discrete wavelet transform for spectra processing, the method serves well to expose typical hazardous molecular species in milk products, such as melamine, sodium thiocyanate and lincomycin hydrochloride, with little sample preparation. The detection sensitivity for melamine, sodium thiocyanate, and lincomycin hydrochloride are 0.1 mg kg-1, 1 mg kg-1, and 0.1 mg kg-1, respectively. Theoretically, we establish that the SDRI represents a novel and environment-friendly method that screens the milk safety efficiently, which could be well extended to inspection of other food safety.


Assuntos
Contaminação de Alimentos/análise , Análise de Perigos e Pontos Críticos de Controle/métodos , Leite/química , Análise Espectral Raman/métodos , Animais , Tiocianatos/análise , Triazinas/análise
5.
Guang Pu Xue Yu Guang Pu Fen Xi ; 37(2): 491-6, 2017 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-30280541

RESUMO

In this paper, two-dimensional (2D) correlation spectroscopy analysis was applied to investigate the influence of the main component in blood and the systematic drift during the measurement on the specificity of glucose in the near-infrared (NIR) spectroscopy. First, the NIR transmittance of glucose aqueous solutions was measured and the 2D correlation NIR spectra were calculated under the perturbation of glucose concentration. Based on the comparative analysis for synchronous and asynchronous 2D correlation spectra, the characteristic absorption peaks of glucose in the combination band and the overtone band were determined. Then a small amount of albumin was added into glucose aqueous solutions, and the transmittance was recorded to perform 2D correlation spectroscopy analysis under the perturbation of glucose concentration. However, the absorption of glucose in the first overtone band (1590nm) and second overtone band (1195nm) was no longer homologous in the 2D correlation spectra, which means that the albumin may reduce the specificity of glucose. Further, the oral glucose tolerance test of healthy volunteer was conducted and the NIR diffuse reflectance of left palm was collected in vivo. The 2D correlation analysis results showed that, the homology of glucose in the diffuse reflectance was also destroyed. Moreover, as the spectral variation from the glucose concentration change is too low to be covered by that induced by systematic drift easily, some background correction methods were usually required. For the transmittance experiment of glucose aqueous solutions and the diffuse reflectance experiment of human body, the pure water sample and 5% diffuse reflectance standard were used as the reference, respectively. Then 2D correlation spectroscopy was developed under the perturbation of measurement time. Results showed that, smaller band shift was observed in the slice spectra of 2D correlation synchronous spectra after the corresponding background correction, and the specificity of glucose was improved both in the in vitro and in vivo experiments. So for the non-invasive glucose sensing by NIR spectroscopy, the wavelengths should be chosen carefully to avoid the absorption band of some interfering components which may destroy the homology of glucose and make spectral interpretation more complicated. And the selection of reference samples for relative measurement is also important to improve the specificity of glucose.


Assuntos
Glicemia/análise , Espectroscopia de Luz Próxima ao Infravermelho , Humanos , Água
6.
Guang Pu Xue Yu Guang Pu Fen Xi ; 36(2): 561-6, 2016 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-27209769

RESUMO

The measurement of tissue optical parameters is the focusing research content of Biomedical Photonics. The optical properties of human tissue are closely related to the physiological and pathological state. In recent years, the tissue imaging diagnosis and non-invasive detection of componentsbecome the hot research topics, applying the tissue optical properties especially the absorption and scattering properties. These provide the basis for the study of optical imaging and the spectrum detection of body composition etc. The Double-Integrating-Spheres (DIS) method can measure the absorption coefficient, scattering coefficient and so on in vitro tissuesimultaneously. It has the advantages of accurate, rapid, large applicable scope. The method applya standard method for measuring the optical parameters. This paper build the wide spectrum measurement system of optical parameters based on DIS and super continuum lasers. Then we analyze the transfer function, error sources and the best measuring conditions of the system. Finally we establish the correction forward model based on BP-MCML and the inverse algorithm of the optical parameters based on L-M algorithm. The optical parameters of intralipid solution in the wavelength range of 1,100~1,400 nm are measured. The experiment results show that the improved inverse algorithm is accurate. The multiple measurements standard deviation is within 3%. Compared the results of scattering coefficient and absorption coefficient at different wavelengths to the results of other research groups, the deviation is less than 3.4%.


Assuntos
Composição Corporal , Análise Espectral , Algoritmos , Humanos , Modelos Teóricos
7.
Guang Pu Xue Yu Guang Pu Fen Xi ; 36(5): 1532-6, 2016 May.
Artigo em Chinês | MEDLINE | ID: mdl-30001058

RESUMO

The determination of tissue optical properties is the fundamental research field in biomedical optics. The ability to separately quantify absorption and scattering coefficients of tissue based on diffuse reflectance spectrum not only helps to gain physiological and structural properties of tissue but also provide insight into the mechanisms of tissue, which leads to the improvement in non-invasive detecting, image diagnosis and photodynamic therapy. In the paper, a flexible and rapid method is developed to extract the absorption and reduced scattering coefficients of turbid medium such as human tissue with diffuse reflectance spectrum. The diffuse reflectance spectrum is measured by the system which includes a white light source, a spectrometer, and a fiber optic probe for delivery and collection of light. The collection efficiency and system transfer function are researched based on the fiber probe geometry. This paper outlines a method based on empirical forward model and non-linear modeling inverse model to extract the optical properties from diffuse reflectance spectrum. The approach includes four steps: (1) generating diffuse reflectance spectra for training inverse model; (2) training the inverse model; (3) measuring and processing the diffuse reflectance spectra of samples; (4) predicting the optical properties of samples. Since the forward and inverse models could be regarded as non-linearity, the Artificial Neural Networks (ANN) is employed to develop the forward and inverse models. The principal component analysis (PCA) is also employed in the inverse model to decompress the data dimension and suppress the spectral noise. With a single fiber optic probe and spectroscopy system, the diffuse reflectance spectrum is measured and preprocessed. The accuracy and robustness of this method are evaluated by measuring the phantoms with a wide range of optical properties. The results indicate that the absorption and scattering coefficients could be extracted accurately by measuring the diffuse reflectance spectrum of single source-detector distance. The mean RMS percentage error is 4.58% and 7.92%, respectively. As to the application of extracting concentration of different chromosphere, it is better to include the absorption peak of every chromosphere within the measuring wavelength range. This method is valid for a wide range of optical properties with the advantage of rapid measurement and simple system setup, which is important for the clinical application.

8.
Guang Pu Xue Yu Guang Pu Fen Xi ; 36(6): 1706-11, 2016 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-30052376

RESUMO

The sodium chloride (NaCl) salt has been reported to be associated with glucose metabolism. However, the effect of it on non-invasive detection of blood glucose using near-infrared spectroscopy is still an open question. The aim of this study was to investigate this affection through transform background correction analysis two-dimensional (2D) correlation synchronous spectrum and the partial least-squares (PLS) regression. First, the transmittances of glucose aqueous solutions with different NaCl content are collected and the pure water and NaCl aqueous solution are measured as the background. Results show that, the dissolving of NaCl in water changes the amplitude and position of the absorption peak of water. There are two negative peaks in 1 400 and 1 500~1 700 nm corrected spectra of NaCl aqueous obviously and the amplitude of peaks associated with NaCl concentration. That's because NaCl affect the molecular binding and vibration of water. Then the glucose aqueous solutions without NaCl and with NaCl are corrected by the spectra of pure water and NaCl aqueous solution, respectively. So we get the conclusion that NaCl also affect the combination of glucose and water molecules. And the two-dimensional correlation spectroscopy analysis is performed under the perturbation of glucose concentration. The slice spectra of synchronous correlation spectra show that, the adding of NaCl weakens the spectral variation due to glucose concentration change in the wavelength of 1 400 and 1 520~1 700 nm. Finally, the partial least square (PLS) regression models were built to quantitatively conduct the influence of NaCl on glucose prediction accuracy. Comparison results showed that, NaCl molecule in aqueous solution will deteriorate the model accuracy, where root mean square error of prediction increases with the NaCl content; the mean difference of predicted glucose concentration between models based on glucose aqueous solutions with NaCl and without NaCl, is linear with NaCl concentration in samples.

9.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 38(6): 746-749, 2016 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-28065246

RESUMO

Chemokine-like factor super family member (CMTM) is a novel generic family firstly reported by Peking University Center for Human Disease Genomics. CMTM8 is one member of this family and has exhibited tumor-inhibiting activities. It can encode proteins approaching to the transmembrane 4 superfamily. CMTM8 is down-regulated in most carcinoma cell lines and tissues. Over-expression of CMTM8 may inhibit the proliferation,migration,and invasion of carcinoma cells. However,the exact mechanism of its anti-tumor activity remains unclear. CMTM8 may be involved in various signaling pathways governing the occurrence and development of tumors. CMTM8 may be a new target in the gene therapies for tumors,while further studies on CMTM8 and its anti-tumor mechanisms are warranted.


Assuntos
Quimiocinas/metabolismo , Proteínas com Domínio MARVEL/metabolismo , Neoplasias/metabolismo , Transdução de Sinais , Regulação para Baixo , Humanos
10.
Rev Sci Instrum ; 86(8): 084902, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26329222

RESUMO

The near infrared (NIR) spectroscopy analytical technique is one of the most advanced and promising tools in many domains. NIR acquisition is easily influenced by temperature, thereby affecting qualitative and quantitative analyses. In this paper, a temperature compensation model was established between NIR signals and output voltage values based on two-dimensional regression analysis. The effectiveness of the proposed compensation scheme was experimentally demonstrated by the measurement of six super luminescent diode sources at 293-313 K. The coefficient of variation was decreased 2-fold with this compensation algorithm. The results indicated that it was suitable for various NIR spectral acquisition systems with lower complexity and a higher signal-noise-ratio after being applied to an acousto-optic-tunable-filter system.


Assuntos
Algoritmos , Espectroscopia de Luz Próxima ao Infravermelho/instrumentação , Temperatura Ambiente , Acústica , Dispositivos Ópticos , Análise de Regressão
11.
Asian J Androl ; 17(6): 1017-21, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25926603

RESUMO

Percent free prostatic-specific antigen (%fPSA) has been introduced as a tool to avoid unnecessary biopsies in patients with a serum PSA level of 4.0-10.0 ng ml-1 , however, it remains controversial whether %fPSA is effective in PSA range of 10.1-20.0 ng ml-1 in both Chinese and Western population. In this study, the diagnostic performance of %fPSA and serum PSA in predicting prostate cancer (PCa) and high-grade PCa (HGPCa) was analyzed in a multi-center biopsy cohort of 5915 consecutive Chinese patients who underwent prostate biopsy in 22 hospitals across China from January 1, 2010 to December 31, 2013. The indication for biopsy was PSA>4.0 ng ml-1 or/and suspicious digital rectal examination. Total and free serum PSA determinations were performed by three types of electrochemiluminescence immunoassays with recalibration to the World Health Organization standards. The diagnostics accuracy of PSA, %fPSA and %fPSA in combination with PSA (%fPSA + PSA) was determined by the area under the receivers operating characteristic curve (AUC). %fPSA was more effective than PSA in men aged ≥60 years old. The AUC was 0.584 and 0.635 in men aged ≥60 years old with a PSA of 4.0-10.0 ng ml-1 and 10.1-20.0 ng ml-1 , respectively. The AUC of %fPSA was superior to that of PSA in predicting HGPCa in patients ≥60 years old in these two PSA range. Our results indicated that %fPSA is both statistically effective and clinical applicable to predict prostate biopsy outcome in Chinese patients aged ≥60 years old with a PSA of 4.0-10.0 ng ml-1 and 10.1-20.0 ng ml-1 .


Assuntos
Biomarcadores Tumorais/sangue , Carcinoma/sangue , Calicreínas/sangue , Antígeno Prostático Específico/sangue , Próstata/patologia , Neoplasias da Próstata/sangue , Idoso , Grupo com Ancestrais do Continente Asiático , Biópsia com Agulha de Grande Calibre , Carcinoma/diagnóstico , Carcinoma/patologia , China , Exame Retal Digital , Endossonografia , Humanos , Biópsia Guiada por Imagem , Masculino , Pessoa de Meia-Idade , Tamanho do Órgão , Neoplasias da Próstata/diagnóstico , Neoplasias da Próstata/patologia , Curva ROC , Estudos Retrospectivos , Sensibilidade e Especificidade
12.
Guang Pu Xue Yu Guang Pu Fen Xi ; 35(2): 547-51, 2015 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-25970930

RESUMO

There are some challenges in near-infrared non-invasive blood glucose measurement, such as the low signal to noise ratio of instrument, the unstable measurement conditions, the unpredictable and irregular changes of the measured object, and etc. Therefore, it is difficult to extract the information of blood glucose concentrations from the complicated signals accurately. Reference measurement method is usually considered to be used to eliminate the effect of background changes. But there is no reference substance which changes synchronously with the anylate. After many years of research, our research group has proposed the floating reference method, which is succeeded in eliminating the spectral effects induced by the instrument drifts and the measured object's background variations. But our studies indicate that the reference-point will changes following the changing of measurement location and wavelength. Therefore, the effects of floating reference method should be verified comprehensively. In this paper, keeping things simple, the Monte Carlo simulation employing Intralipid solution with the concentrations of 5% and 10% is performed to verify the effect of floating reference method used into eliminating the consequences of the light source drift. And the light source drift is introduced through varying the incident photon number. The effectiveness of the floating reference method with corresponding reference-points at different wavelengths in eliminating the variations of the light source drift is estimated. The comparison of the prediction abilities of the calibration models with and without using this method shows that the RMSEPs of the method are decreased by about 98.57% (5%Intralipid)and 99.36% (10% Intralipid)for different Intralipid. The results indicate that the floating reference method has obvious effect in eliminating the background changes.


Assuntos
Glicemia/análise , Método de Monte Carlo , Calibragem , Luz , Modelos Teóricos , Fótons , Padrões de Referência
13.
Guang Pu Xue Yu Guang Pu Fen Xi ; 34(3): 605-8, 2014 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-25208374

RESUMO

In the glucose measuring technique by surface plasmon resonance, D-galactose/D-glucose binding protein (GGBP) that can specifically adsorb glucose was introduced, and high-precision specific detection of glucose concentration was realized. In the present paper, the GGBP protein was bound on the surface of SPR sensor through thiol coupling method. GGBP binding experiment was carried out on SPR sensor and then glucose concentration experiment was conducted with this sensor. The results indicated that the SPR sensor had good linearity, stability and repeatability in the range of 0.1-10 mg x dL(-1). SPR sensor bound with GGBP would have great potential and vast development prospects.


Assuntos
Glucose/análise , Ressonância de Plasmônio de Superfície , Galactose , Proteínas de Transporte de Monossacarídeos , Ligação Proteica , Compostos de Sulfidrila
14.
Beijing Da Xue Xue Bao Yi Xue Ban ; 46(4): 519-23, 2014 Aug 18.
Artigo em Chinês | MEDLINE | ID: mdl-25131461

RESUMO

OBJECTIVE: To investigate the expression of urinary brainderived neurotrophic factor (BDNF) in benign prostatic hyperplasia patients with overactive bladder (OAB) symptoms and its correlation with the severity of OAB symptoms. METHODS: According to the inclusion and exclusion criteria, a total of 178 patients with benign prostatic hyperplasia who were to undergo transurethral resection of prostate (TURP) were enrolled in this study. All the patients had accepted basic preoperative evaluations, as well as an assessment of their International Prostate Symptom Score (IPSS) and Overactive Bladder Symptom Score (OABSS). The patients who had been scheduled for surgery had to take the urodynamic assessment. Urinary BDNF levels were measured by the enzyme-linked immunosorbent assay (ELISA) and the results were further normalized to the concentration of urinary creatinine (BDNF/Cr, mg/mol). RESULTS: The urinary BDNF/Cr levels of the patients with moderate and severe lower urinary tract symptoms were (1.189 ± 0.753) mg/mol and (1.817 ± 1.110) mg/mol (P < 0.001). The urinary BDNF/Cr levels of the patients with grades III-VI obstruction were (1.382 ± 0.945) mg/mol, (1.435 ± 0.938) mg/mol, (1.640 ± 1.104) mg/mol, and (1.653 ± 1.019) mg/mol, respectively (P > 0.05). There was no correlation between the urinary BDNF/Cr levels and the severity of obstruction (r = 0.103, P = 0.173). The urinary BDNF/Cr levels in the patients with and without OAB symptoms were (1.913 ± 0.843) mg/mol and (0.297 ± 0.183) mg/mol (P < 0.001). The urinary BDNF/Cr levels in the patients with mild, moderate and severe OAB symptoms were (1.501 ± 0.543) mg/mol, (1.806 ± 0.703) mg/mol and (2.560 ± 0.979) mg/mol, respectively (P < 0.05). There was a correlation between the urinary BDNF/Cr levels and the severity of OAB symptoms (r = 0.743, P < 0.001). The urinary BDNF/Cr levels in the patients with urodynamic detrusor overativity were significantly higher than those without detrusor overativity [(1.917 ± 0.866) mg/mol and (1.194 ± 1.013) mg/mol, P < 0.001]. CONCLUSION: There is no correlation between urinary BDNF and severity of obstruction in benign prostatic hyperplasia patients with moderate and severe lower urinary tract symptoms. The urinary BDNF levels in patients with OAB symptoms are elevated compared with patients without OAB symptoms, and are correlated with the severity of OAB symptoms.


Assuntos
Fator Neurotrófico Derivado do Encéfalo/urina , Sintomas do Trato Urinário Inferior/metabolismo , Hiperplasia Prostática/metabolismo , Bexiga Urinária Hiperativa/metabolismo , Creatinina/urina , Ensaio de Imunoadsorção Enzimática , Humanos , Masculino , Urodinâmica
15.
Beijing Da Xue Xue Bao Yi Xue Ban ; 46(4): 570-3, 2014 Aug 18.
Artigo em Chinês | MEDLINE | ID: mdl-25131473

RESUMO

OBJECTIVE: To investigate the change of the female patients' sexual function after tension free vaginal tape (TVT) or transobturator suburethral tape (TVT-O) surgery for stress urinary incontinence (SUI). METHODS: Female sexual function index (FSFI) was sent to 66 female patients who underwent a TVT/TVT-O surgery at least 3 months after the operation, to a maximum of 5 years. All The patients were divided into two groups according to different surgical methods, different follow-up intervals (more than or less than 1 year) and whether there was concomitant coital incontinence (CCI) before the operation. The changes of FSFI scores before and after the operation were compared between the two groups by multiple covariances analysis. The total score was higher, and the sexual function was better. RESULTS: In the study, 60 patients answered the questionnaire, and 51 questionnaires were available. Before surgery, there were no significant difference between TVT and TVT-O groups in FSFI, age, body mass index (BMI). As to patients who had TVT/TVT-O surgery, when the influence of the postoperative period and symptoms accompanying intercourse were corrected, the two groups had no significant difference in difference of FSFI (dFSFI) scores after operation (F = 2.52, P = 0.119), and they had similar sexual function improvement ratio (40.0% vs. 44.4%, P > 0.05). But to the patients who had concomitant coital incontinence before the operations, the dFSFI scores was higher than patients who did not have concomitant coital incontinence (1.86 ± 1.95 vs. -0.09 ± 2.24, P < 0.05). CONCLUSION: TVT and TVT-O surgery have no significant difference in influence on sexual function in women between each other, but the patients who suffer from CCI before the surgery will have a better sexual function improvement.


Assuntos
Qualidade de Vida , Slings Suburetrais , Incontinência Urinária por Estresse/cirurgia , Coito , Feminino , Humanos , Período Pós-Operatório , Inquéritos e Questionários , Saúde da Mulher
16.
Guang Pu Xue Yu Guang Pu Fen Xi ; 34(10): 2775-8, 2014 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-25739224

RESUMO

Measuring the glucose concentrations in the interstitial fluid is currently the main method to achieve the continuous blood glucose monitoring. The MIR-ATR(Mid-infrared, Attenuated Total Reflection)Spectroscopy has prominent advantage on the analysis of small biological molecule for composition information like the glucose, but it is still an unresolved problem that how to detect the subcutaneous glucose concentration by using the MIR-ATR Spectroscopy. In the present paper, we carry out the experiment based on MIR-ATR for the detection of subcutaneous glucose information on both the natural state and the penetration state based on the theoryanalysis of MIR penetration depth. Firstly, collect spectral data of the subcutaneous glucose concentration of human finger on the natural state were collected as the light shined the skin directly, and it was discussed whether the MIR can penetrate the skin to get the information of subcutaneous glucose. On this basis, collect spectral data of the subcutaneous glucose concentration of human finger at the penetration state were collected when the Interstitial fluid is permeated to the surface layer by using low-frequency ultrasound and vacuum, then it analyzed that whether it can detect the glucose-specific information or not. As the two-dimensional correlation spectroscopy has high resolution and good versatility, it is widely used to analyze the inter-molecular reaction and judge the absorption peaks information in many fields including the MIR spectroscopy field, so we choose the Two-dimensional correlation spectroscopy to analyze the information of subcutaneous glucose concentration at the natural state and the penetration state. The experiment result shows that the MIR-ATR spectroscopy can't be applied in the detection of subcutaneous glucose concentrationdirectly, and it is a promising direction to make the Interstitial fluid permeated to the surface layer by the physical methods or chemical methods.


Assuntos
Líquido Extracelular/química , Glucose/análise , Humanos , Pele , Espectrofotometria Infravermelho
17.
Zhonghua Yi Xue Za Zhi ; 93(34): 2719-22, 2013 Sep 10.
Artigo em Chinês | MEDLINE | ID: mdl-24360105

RESUMO

OBJECTIVE: To examine the prevalence of human papilloma virus (HPV) in penile squamous-cell carcinomas (SCCs), explore the relationship between HPV and clinicopathological variables and determine its value for predicting disease-specific survival. METHODS: We retrospectively collected clinicopathological data of 28 patients with penile squamous cell carcinomas from 2000 to 2009 at Beijing Cancer Hospital, Beijing Hospital and Peking University People's Hospital. Polymerase chain reaction (PCR) was used to detect the presence of HPV DNA in tumor specimens. Regular follow-ups were conducted. Disease-specific survival plots were drawn with the Kaplan-Meier method and compared with the Log-Rank test. Cox proportional hazard analysis was applied to assess the independent effects of several prognostic factors on survival. RESULTS: HPV DNA was detected in 7/28 samples. After sequencing, all 7 samples were confirmed to be HPV-16 type. The median follow-up period was 60 (6-150) months. By the time of analysis, 8/28 patients died. HPV DNA was not significantly associated with age, pathological grading, pathologic T stage or lymph node metastasis (P = 0.191, 0.165, 1.000, 0.639 respectively). Cox multivariate regression analysis indicated that lymph node status (HR = 5.5, P = 0.023) was an independent predictive factor of disease specific survival, followed by pathological T classification (HR = 11.0, P = 0.035) . The 5-year disease-specific survival in patients with HPV DNA positive was higher than that in those with HPV DNA negative (67% vs 58%) . But survival had no significant differences (P = 0.431). CONCLUSIONS: Higher pathologic T stage and lymph node metastasis are independent prognostic factors for worse survival. And mortality does not increase markedly in penile cancer patients with HPV DNA positive.


Assuntos
Papillomaviridae , Neoplasias Penianas/patologia , Neoplasias Penianas/virologia , Adulto , Idoso , Idoso de 80 Anos ou mais , DNA Viral/isolamento & purificação , Humanos , Masculino , Pessoa de Meia-Idade , Papillomaviridae/genética , Prognóstico , Estudos Retrospectivos
18.
Guang Pu Xue Yu Guang Pu Fen Xi ; 33(8): 2083-6, 2013 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-24159851

RESUMO

Based on the method of kernet Orthogonal Projection to Latent Structure Discriminant Analysis, discrimination models for adulterated milk were established in the present paper. Forty adulterated milk samples with melamine (0.01-3 g x L(-1)) and 40 adulterated milk samples with urea (1-20 g x L(-1)) were prepared, respectively. Then the near-infrared absorption spectra of all samples were measured. The spectra in the range of 4 200-4 800 cm(-1) were selected to construct the KOPLS-DA models for milk adulterated with melamine, milk adulterated with urea and milk adulterated with both melamine and urea. The results showed that, compared with PLS-DA and OPLS-DA models, KOPLS-DA model had better discriminant ability for the adulterated milk, and its classification accuracy rate (CAR) for milk adulterated with melamine, milk adulterated with urea and milk adulterated with both melamine and urea were 95%, 100% and 97.5%, respectively.


Assuntos
Análise de Alimentos/métodos , Contaminação de Alimentos/análise , Leite/química , Espectroscopia de Luz Próxima ao Infravermelho , Animais , Bovinos , Análise Discriminante , Análise dos Mínimos Quadrados , Modelos Teóricos , Triazinas/análise , Ureia/análise
19.
Beijing Da Xue Xue Bao Yi Xue Ban ; 45(4): 537-41, 2013 Aug 18.
Artigo em Chinês | MEDLINE | ID: mdl-23939157

RESUMO

OBJECTIVE: To investigate the expression and clinical pathological significance of CMTM8 and E-cadherin in primary and metastatic clear-cell renal cell carcinoma. METHODS: The immunohistochemistry was used to detect the expressions of CMTM8 and E-cadherin in 17 cases of primary clear-cell renal cell carcinoma, 8 cases of normal renal tissue, 9 cases of metastatic renal cell carcinoma in lungs and 10 cases of metastatic renal cell carcinoma in bones. RESULTS: The membranous staining intensities of CMTM8 and E-cadherin in normal renal tissue were strong, but reduced in low-grade clear-cell renal cell carcinoma. There were positive cytoplasmic stainings of CMTM8 and E-cadherin in high-grade clear-cell renal cell carcinoma. Increased cytoplasmic expression of CMTM8 was frequent in metastatic renal cell carcinoma, accompanied with reduced cell surface staining. The expression of E-cadherin could be negative or weakly positive at membrane and cytoplasma. CMTM8 and E-cadherin expressions were negatively correlated with development and metastasis in clear-cell renal cell carcinoma (r=-0.841 and r=-0.732, P<0.001). Moreover, CMTM8 was also correlated with the expression of E-cadherin (r=0.694, P<0.001). CONCLUSION: CMTM8 and E-cadherin are negatively correlated with tumorgenesis and development in clear-cell renal cell carcinoma. The location and intensity of their expressions have significant association with the prognosis.


Assuntos
Caderinas/metabolismo , Carcinoma de Células Renais/metabolismo , Quimiocinas/metabolismo , Proteínas com Domínio MARVEL/metabolismo , Antígenos CD , Neoplasias Ósseas/metabolismo , Neoplasias Ósseas/secundário , Caderinas/genética , Carcinoma de Células Renais/patologia , Quimiocinas/genética , Humanos , Imuno-Histoquímica , Rim/metabolismo , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/secundário , Proteínas com Domínio MARVEL/genética , Prognóstico
20.
Guang Pu Xue Yu Guang Pu Fen Xi ; 33(4): 972-6, 2013 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-23841410

RESUMO

The authors proposed a method of control and stabilization for laser emission wavelengths and power, and presented the mid-infrared wavelength tunable laser with broad emission spectrum band of 9.19-9.77 microm, half wave width of 4 cm(-1), spectral resolution of 2.7 x 10(4) and max power of 800 mW with fluctuation < 0.8% in the present paper. The tunable laser was employed as the light source in combination with ATR sensor for glucose measurement in PBS solution. In our experiments, absorbance at the five laser emission wavelengths, including 1 081, 1 076, 1 051, 1 041 and 1 037 cm(-1) in the 9R and 9P band of the laser emission spectrum, all correlates well with the glucose concentration (R2 > 0.99, SD < 0.0004, P < 0.000 1). Especially, the sensitivity of this laser spectroscopy system is about 4 times as high as that of traditional FTIR spectrometer.


Assuntos
Glucose/análise , Raios Infravermelhos , Lasers , Fotometria/instrumentação , Glicemia/análise , Dióxido de Carbono , Desenho de Equipamento , Fotometria/métodos , Espectroscopia de Infravermelho com Transformada de Fourier/métodos
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