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1.
Artigo em Inglês | MEDLINE | ID: mdl-32044811

RESUMO

STUDY DESIGN: A prospective case-control study. OBJECTIVES: To investigate whether the asymmetric changes are primary or secondary to spinal deformity. SUMMARY OF BACKGROUND DATA: Previous study reported significantly decreased expression of Wnt/B-catenin pathway in adolescent idiopathic scoliosis (AIS) patients. To date, there is a lack of study investigating the relationship between differentially expressed Wnt/B-catenin pathway and the onset of the curve. METHODS: Paraspinal muscles were collected from 40 female AIS patients and 20 age-matched congenital scoliosis (CS) patients. For CS patients, the samples were collected from concave side and the convex side at the apical region. For AIS patients, the samples were collected from the proximal bilateral sides of the spine in addition to the apical region. qPCR and western blot were used to determine the expression of LBX1, B-catenin, and PAX3, all of which are regulated by the Wnt/B-catenin pathway. The relative mRNA expression level between the concave and the convex side was performed with the Student t test. Pearson correlation analysis was used to determine the relationship between gene expression and the curve magnitude. RESULTS: AIS patients were found to have remarkably lower mRNA and protein expression of B-catenin, LBX1 and PAX3 in the concave side than in the convex side at the apical region. By contrast, at the proximal region, the mRNA expression of these 3 genes was comparable. Moreover, no significant difference regarding mRNA expression was found between the concave side and the convex side of CS patients. There was no remarkable correlation between the mRNA expression of the 3 genes and Cobb angle. CONCLUSIONS: There exists remarkably asymmetric expression of Wnt/B-catenin pathway at the apical region of AIS, which however was comparable at the apical region of CS patients. Further investigation of Wnt/B-catenin signaling pathway may help reveal the etiology of AIS in future study. LEVEL OF EVIDENCE: 4.

2.
Int J Mol Med ; 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31985021

RESUMO

Acute kidney injury (AKI) is characterized by abrupt kidney dysfunction. It results in remote organ dysfunction, including the brain. The underlying mechanism of the kidney­brain axis in AKI and effective protective approaches remain unknown. The present study aimed to investigate the potential protective effect of ginsenoside (GS) on AKI induced by glycerol in rats. Kidney function was initially assessed by blood urea nitrogen (BUN) and creatinine (Cre) tests, and was identified to be severely impaired following glycerol treatment, based on significant increases in BUN and Cre levels observed. Severe extensive necrosis of the majority of the renal tubules was observed by hematoxylin and eosin staining, additionally confirming that glycerol induced AKI. GS was identified to ameliorate the impairment of kidney function in the context of AKI. Further investigation of the mechanism revealed that GS may induce protection against oxidative stress via a kidney­brain axis. Furthermore, GS improved the activation of hypoxia­inducible factor 1α (HIF­1α) and vascular endothelial growth factor A (VEGF­A) in the hypothalamus response to AKI, and in the kidney tissues. The protective effect of GS in AKI may be associated with the interaction between the kidney and the brain. Taken together, these results suggested that GS was involved in the protective effects against AKI by decreasing oxidative damage to the kidney and brain, and by upregulating HIF­1α and VEGF­A levels in the kidney­brain axis.

3.
Spine (Phila Pa 1976) ; 45(4): 226-233, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31513097

RESUMO

STUDY DESIGN: A genetic case-control association study. OBJECTIVE: The aim of this study was to investigate the association of SLC39A8 with the susceptibility of adolescent idiopathic scoliosis (AIS) in Chinese Han population. SUMMARY OF BACKGROUND DATA: A recent exome-wide association study identified a missense variant rs13107325 in SLC39A8 that was associated with AIS. However, there was a lack of study validating the association of this novel mutation with AIS in other populations. METHODS: The variant rs13107325 was genotyped in 965 AIS patients and 976 healthy controls by allelic specific multiple ligase detection reactions. Variants located in the coding region of SLC39A8 were identified by exon sequencing for 192 AIS patients and 192 controls. Paraspinal muscles from 36 AIS patients and 36 age-matched congenital scoliosis patients were collected for the gene expression analysis. Comparison between the cases and controls was performed with the χ test for genotyping data or with Student t test for gene expression analysis. RESULTS: For the missense variant rs13107325, there was no case of mutation detected in the patients or the controls. All the subjects had homozygous genotype CC. Exon sequencing revealed that a coding variant rs11097773 of SLC39A8 had a significantly different distribution of minor allele frequency between patients and controls (7.81% vs. 14.8%, P = 0.002). The mRNA expression of SLC39A8 in the patients was remarkably lower than that in the controls (0.0015 ±â€Š0.00026 vs. 0.0021 ±â€Š0.00033, P < 0.001). CONCLUSION: The association of previously reported novel mutation (rs13107325 in SLC39A8) with AIS was not replicated in the Chinese population. Interestingly, a novel coding variant rs11097773 of SLC39A8 is found significantly associated with AIS. Moreover, the expression of SLC39A8 was obviously decreased in AIS patients. Further study is warranted to clarify the functional role of rs11097773 in the development of AIS. LEVEL OF EVIDENCE: 3.

4.
Spine (Phila Pa 1976) ; 45(3): E120-E125, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31513102

RESUMO

STUDY DESIGN: A case-control study. OBJECTIVE: This study aimed to investigate the potential role of PIEZO2 gene in the development of AIS. SUMMARY OF BACKGROUND DATA: Mutations of PIEZO2 gene have been reported to be associated with progressive scoliosis and impaired proprioception. Previous studies showed that patients with AIS may have impaired proprioception. However, there is lack of knowledge concerning the mechanism underlying the proprioception of AIS patients and the role of PIEZO2 gene in the etiology of AIS. METHODS: Proprioception tests were performed in both AIS patients and age-matched healthy controls. Based on the falling risk scores, AIS patients were divided into impaired proprioception group and unimpaired proprioception group. Paraspinal muscle was collected from 34 AIS patients during surgery. The tissue expression of PIEZO2 was compared between the impaired group and the unimpaired group. In addition, the average number of muscle fibers in the muscle spindle was compared between the two groups. RESULTS: Proprioception test showed that patients had significantly higher falling index (41.7 ±â€Š16.5 vs. 11.3 ±â€Š8.3, P = 0.004). In addition, the expression of PIEZO2 gene was remarkably decreased in the impaired group (0.51 ±â€Š0.24 vs. 1.00 ±â€Š0.33, P = 0.04). The average number of muscle fibers in the muscle spindle was significantly decreased in AIS patients of the impaired group than those of the unimpaired group (2.2 ±â€Š1.3 vs. 3.5 ±â€Š2.1, P = 0.04). PIEZO2 expression level was remarkably correlated with the average number of muscle fibers in the muscle spindle (r = 0.352, P = 0.04). CONCLUSION: Proprioception is remarkably impaired in patients with AIS. Abnormal expression of PIEZO2 may play a role in AIS via altered proprioception and number of muscle fibers in the muscle spindles. Further investigation is warranted to illustrate the mechanism regulating PIEZO2 expression in AIS. LEVEL OF EVIDENCE: 4.

5.
ACS Nano ; 14(1): 406-414, 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31860277

RESUMO

Inspired by chasing-escaping behaviors of predator and swarming prey in nature, here we demonstrate a concept to create active micromotor systems from two species of passive microparticles with biomimetic predator-prey interactions. In this concept, the biomimetic predator-prey interactions are established in a binary particle system comprising the diffusiophoretic attractive microparticles (prey particles) and the diffusiophoretic repulsive ones (predator particles). In the absence of additional chemical fuels and external fields, the predator particles are attracted by and constantly chase the swarming prey particles, which, in response, escape from the former and show dynamic group reconfigurations because of the local repulsion. Based on this concept, various synthetic active micromotor systems have been demonstrated, including active ZnO-TiO2, Ag3PO4-TiO2, and ZnO-AgBr micromotor systems. As the predator and prey particles are powered by each other through the biomimetic predator-prey interactions, the concept proposed here provides an advanced method to develop not only a class of single micromotors powered by passive particles or "solid fuels" but also micromotor swarms capable of manipulating "moving cargo". In addition, it also illustrates a proof-of-concept implementation of intelligent micro/nanomotor systems composed of heterogeneous individuals with complementary or cooperative functions.

6.
Int J Infect Dis ; 89: 163-168, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31585217

RESUMO

OBJECTIVE: Spinal hydatid disease is rare and remains a serious health problem associated with high rates of recurrence. We report our experience in treating patients with thoracic spinal hydatidosis through a single-center case-series study. METHODS: Sixteen patients with thoracic spinal hydatidosis were treated in our center between 1995 and 2017. A total en bloc spondylectomy (TES) was performed in three patients. Five patients were treated with posterior decompression and stabilization after removing the involved elements. The remaining patients underwent curettage and resection of the infected bone. The therapy was completed with medical treatment or radiotherapy. RESULTS: Of the 16 patients, seven were men and nine were women; their mean age was 38.5 years (range 28-60 years). The infected area was the upper thoracic level in one patient, mid thoracic level in eight patients, and lower thoracic level in seven patients. Four patients had paraplegia and seven had paraparesis before surgery. At the last follow-up, five patients had successfully recovered from the neurological damage. During a mean follow-up of 4.75 years (range 2-12 years), eight patients had local recurrence; however, no patient who underwent TES had recurrence. CONCLUSIONS: An individualized surgical strategy should be decided carefully for each patient in the first intervention. In the early stages of the disease, TES should be considered as a treatment for suitable cases of primary thoracic spinal hydatidosis.

7.
Pain Physician ; 22(5): E457-E465, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31561658

RESUMO

BACKGROUND: Scoliosis secondary to lumbar disc herniation (LDH) may occur in both adolescents and adults. As the spine is more flexible in adolescents than in adults, the curve features and curve evolution could be different between these 2 cohorts, which were unclear. OBJECTIVES: To compare the radiologic features of scoliosis secondary to LDH between adolescents and adults, and to further characterize the curve evolution after lumbar discectomy in 2 cohorts. STUDY DESIGN: A retrospective study. SETTING: An inpatient surgery center. METHODS: Patients with scoliosis secondary to LDH who underwent surgical intervention between 2010 and 2016 were reviewed. Radiographic parameters were measured on standing whole spine radiographs. The apical vertebral translation was measured on serial radiographs taken before surgery, one month and 6 months after surgery, and at last follow-up to evaluate the curve evolution. Meanwhile, the patients' reported outcomes were evaluated. According to age, patients were divided into adolescent and adult group. Comparisons between the 2 groups were made with regards to the preoperative and postoperative radiographic parameters and clinical outcomes. RESULTS: A total of 42 adolescent and 41 adult patients were included in this study. The incidence of scoliosis secondary to LDH in the adolescents was significantly higher than that in the adults. Adolescent patients present remarkably higher incidence of coronal balance as compared with the adult patients preoperatively. No significant difference was observed between the 2 groups in terms of preoperative radiographic parameters. A total of 85.7% of the adolescent patients and 92.7% of the adult patients achieved resolution of scoliosis within 6 months after surgery. LIMITATIONS: This was a retrospective study with a small series of cases and relatively short-term follow-up. CONCLUSIONS: The incidence of scoliosis secondary to LDH in adolescents is significantly higher than in adults. Moreover, adolescent patients are more likely to present coronal balance before surgery. The 2 cohorts could have comparable curve evolution, and resolution of scoliosis generally occurred within 6 months after surgery. KEY WORDS: Sciatic scoliosis, lumbar disc herniation, adolescent, adult, resolution, lumbar discectomy.

8.
Technol Cancer Res Treat ; 18: 1533033819874802, 2019 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-31522615

RESUMO

Variant rs7034162 in NFIB was reported to be associated with metastasis of osteosarcoma in European cases with genome-wide significance. Our purpose was to replicate the association of rs7034162 with the metastasis of osteosarcoma in the Chinese population and to further characterize the expression level of NFIB in osteosarcoma tissues. A total of 321 patients were included in this study. Variant rs7034162 was genotyped for each patient using the Taqman genotyping assay. Fifty-two cases of tumor tissues and adjacent normal tissues were collected during surgery. The χ2 test was used to investigate the association of rs7034162 with the metastasis of osteosarcoma. The Student t test was used to compare the gene expression between patients with metastasis and those without metastasis. The messenger RNA expression level of NFIB was then compared among different genotypes of rs7034162 with 1-way analysis of variance test. Ninety-three patients were found to have metastasis. Patients with genotype AA had remarkably higher incidence of metastasis than those with genotype TT (34.4% vs 17.1%, P = .002). Patients with metastasis were found to have significantly higher rate of allele A than those without metastasis (53.2% vs 43.9%, P = .03). The messenger RNA expression of NFIB was significantly lower in tumor tissues of patients with metastasis than in those without metastasis (0.00035 ± 0.00017 vs 0.00063 ± 0.0025, P < .001). Compared to patients with genotype TT, those with genotype AA had remarkably decreased expression of NFIB (0.00033 ± 0.0014 vs 0.00067 ± 0.00037, P = .01). Single-nucleotide polymorphism rs7034162 was associated with metastasis of osteosarcoma in the Chinese population possibly via downregulation of NFIB. Further network analyses revealing the related pathways can help elucidate the molecular mechanism of distant metastasis in patients with osteosarcoma.

9.
iScience ; 19: 415-424, 2019 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-31421596

RESUMO

Inspired by astonishing collective motions and tactic behaviors in nature, here we show phototactic flocking of synthetic photochemical micromotors. When enriched with hydroxyl groups, TiO2 micromotors can spontaneously gather into flocks in aqueous media through electrolyte diffusiophoresis. Under light irradiation, due to the dominant nonelectrolyte diffusiophoretic interaction resulting from the overlap of asymmetric nonelectrolyte clouds around adjacent individuals, these flocks exhibit intriguing collective behaviors, such as dilatational negative phototaxis, high collective velocity, and adaptive group reconfiguration. Consequently, the micromotor flocks can migrate along pre-designed paths and actively bypass obstacles with reversible dilatation (expansion/contraction) under pulsed light navigation. Furthermore, owing to the enhanced driving force and rapid dilatational area covering, they are able to execute cooperative tasks that single micromotors cannot achieve, such as cooperative large-cargo transport and collective microenvironment mapping. Our discovery would promote the creation of reconfigurable microrobots, active materials, and intelligent synthetic systems.

10.
J Mol Cell Biol ; 2019 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-31174204

RESUMO

Error-free cell division depends on the accurate assembly of the spindle midzone from dynamic spindle microtubules to ensure chromatid segregation during metaphase-anaphase transition. However, the mechanism underlying the key transition from the mitotic spindle to central spindle before anaphase onset remains elusive. Given the prevalence of chromosome instability phenotype in gastric tumorigenesis, we developed a strategy to model context-dependent cell division using a combination of light sheet microscope and 3D gastric organoids. Light sheet microscopic image analyses of 3D organoids showed that CENP-E inhibited cells undergoing aberrant metaphase-anaphase transition and exhibiting chromosome segregation errors during mitosis. High-resolution real-time imaging analyses of 2D cell culture revealed that CENP-E inhibited cells undergoing central spindle splitting and chromosome instability phenotype. Using biotinylated syntelin as an affinity matrix, we found that CENP-E forms a complex with PRC1 in mitotic cells. Chemical inhibition of CENP-E in metaphase by syntelin prevented accurate central spindle assembly by perturbing temporal assembly of PRC1 to the midzone. Thus, CENP-E-mediated PRC1 assembly to the central spindle constitutes a temporal switch to organize dynamic kinetochore microtubules into stable midzone arrays. These findings reveal a previously uncharacterized role of CENP-E in temporal control of central spindle assembly. Since CENP-E is absent from yeast, we reasoned that metazoans evolved an elaborate central spindle organization machinery to ensure accurate sister chromatid segregation during anaphase and cytokinesis.

11.
J Orthop Surg Res ; 14(1): 194, 2019 Jun 27.
Artigo em Inglês | MEDLINE | ID: mdl-31248440

RESUMO

BACKGROUND: Infantile patients with congenital scoliosis (CS) can be confronted with increasing risk of mortality and morbidity. To date, the effectiveness of conservative treatment in CS has not been sufficiently investigated. We aimed to evaluate the bracing outcome in patients with CS and to investigate whether wearing brace can effectively delay the surgical procedures. METHODS: A total of 39 braced CS patients including 25 boys and 14 girls were reviewed for the eligibility to be included in this study. Radiographic parameters including curve magnitude and T1 to T12 height were evaluated for each patient at the initiation of the treatment and at the final follow-up (FU), respectively. Duration of the follow-up and requirement of surgical interventions were also recorded. The student t test was used to compare the radiographic parameters between the initial visit and the last FU. RESULTS: The mean initial age at bracing was 4.1 ± 2.3 years, and 7.5 ± 1.8 brace modifications were performed during a mean FU period of 42.1 ± 26.5 months. The mean curve magnitude before bracing was 44.1 ± 12.2°, which was corrected to 41.3 ± 13.5° at the final visit (p = 0.33). T1-T12 height increased from 13.4 ± 2.5 to 17.1 ± 2.8 cm during the treatment (P < 0.001). Nine patients underwent surgical intervention due to the curve progression more than 5°, with the time of surgery delayed for 32.1 ± 18.2 months. CONCLUSIONS: Brace treatment is an effective time-buying modality for CS patients, which may help maintain the body growth and delay the surgical intervention.


Assuntos
Braquetes/tendências , Progressão da Doença , Escoliose/diagnóstico por imagem , Escoliose/terapia , Criança , Pré-Escolar , Feminino , Seguimentos , Humanos , Lactente , Masculino , Estudos Retrospectivos , Fatores de Tempo , Resultado do Tratamento
12.
Sci Rep ; 9(1): 6202, 2019 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-30996275

RESUMO

It is well-documented that melatonin deficiency has been linked to the etiopathogenesis of adolescent idiopathic scoliosis. In this study, we intended to apply melatonin in melatonin-deficient mice to ascertain whether melatonin could reduce the incidence/severity of scoliosis, and investigate the role of melatonin on bone mineral density in scoliosis. A total of 80 mice were divided into 4 groups: 20 quadrupedal mice and 20 bipedal mice served as controls; 20 quadrupedal and 20 bipedal mice received oral melatonin (8 mg/kg BW) daily. After 5th, 10th, 15th and 20th weeks of treatment, radiographs and in vivo micro-CT were used to determine the incidence of scoliosis and bone qualities, respectively. Upon sacrifice, the levels of melatonin were measured in each group. At 20th week, the occurrence of scoliosis was 80%, 30%, 22% and 5% in bipedal, quadrupedal, bipedal + melatonin and quadrupedal + melatonin group, respectively. The trabecular bone quality of the vertebral body was significantly ameliorated in the melatonin-treated bipedal models. Likewise, the number of osteoclasts was significantly less in those treated with melatonin. Our results indicated that melatonin deficiency may be crucial for scoliotic development, and restoration of melatonin levels can prevent scoliotic development with the improvement in bone density.

13.
Chem Asian J ; 14(14): 2503-2511, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-30997736

RESUMO

Extraction of nucleic acids in microsystems is of significance for biomedical applications, but the current extraction methods generally require sophisticated microchannels and external equipment, hindering their practical applications. In this work, we have demonstrated a simple, versatile and efficient approach to extract nucleic acids in microsystems by developing cationic branched polyethyleneimine (PEI)-functionalized tubular micromotors. The as-developed tubular micromotors are fabricated by a two-step process combining the template-assisted electrodeposition and carbodiimide chemistry, and contain an inner catalytic Pt layer, a middle magnetic Ni layer and an outer cationic PEI layer. They exhibit autonomous bubble-propelled motion in aqueous hydrogen peroxide solutions, which can be guided by an external magnetic field, and the surface charges can be reversibly modulated by changing the pH value of the solution. Consequently, the as-developed tubular micromotors can selectively absorb nucleic acids from acidic solutions and desorb them into alkaline solutions, leading to the extraction of nucleic acids with high efficiency without external stirring. Furthermore, they can be operated in a microchannel chip without the aid of a pumping system. Our results indicate that this PEI-functionalized tubular micromotor platform provides a novel, simple and versatile microsystem nucleic acid extraction technology, holding considerable promise for important practical applications.


Assuntos
Técnicas Analíticas Microfluídicas , Ácidos Nucleicos/isolamento & purificação , Técnicas Analíticas Microfluídicas/instrumentação , Ácidos Nucleicos/química , Tamanho da Partícula , Polietilenoimina/química , Propriedades de Superfície
14.
ACS Appl Mater Interfaces ; 11(18): 16639-16646, 2019 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-30990654

RESUMO

The key principle of designing a micro/nanomotor is to introduce asymmetry to a micro/nanoparticle. However, micro/nanomotors designed based on external asymmetry and inherent chemical and geometrical asymmetry usually suffer from tedious small-scale preparation, high cost, and/or complexity of external power and control devices, making them face insurmountable hurdles in practical applications. Herein, considering the possible distinct properties of different polymorphs, we propose a novel design strategy of simple-structured micromotors by introducing inherent asymmetry in crystalline phases. The inherent phase asymmetry can be easily introduced in spherical TiO2 particles by adjusting the calcination temperature to control the phase transition and growth of primary grains. The as-designed anatase/rutile TiO2 micromotors not only show efficient autonomous motions controlled by light in liquid media stemming from the asymmetric surface photocatalytic redox reactions but also have a promising application in environmental remediation due to their high photocatalytic activity in "on-the-fly" degradation of organic pollutants, facile large-scale fabrication, and low cost. The proposed design strategy may pave the way for the large-scale productions and applications of micro/nanomotors.

15.
Spine (Phila Pa 1976) ; 44(18): E1063-E1067, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30994600

RESUMO

STUDY DESIGN: A genetic association study. OBJECTIVE: The aim of this study was to determine whether variants of ABO, SOX6, and CDH13 are associated with the susceptibility of AIS in Chinese Han population. SUMMARY OF BACKGROUND DATA: A recent large-scale genome-wide association study reported three novel loci in CDH13, ABO, and SOX6 genes associated with adolescent idiopathic scoliosis (AIS) in Japanese population. However, the association of these three genes with AIS in other populations remains obscure. METHODS: The SNPs rs4513093, rs687621, and rs1455114 were genotyped in 1208 female patients and 2498 healthy controls. Samples for the expression analysis in paraspinal muscles were collected from 49 AIS and 33 congenital scoliosis (CS) patients during surgical interventions. Chi-square analysis was used to assess the difference regarding genotype and allele frequency between cases and controls. Tissue expressions of ABO, CDH13, and SOX6 were compared between AIS and CS patients by the Student t test. RESULTS: SNPs rs4513093 of CDH13 and rs687621 of ABO were found to be significantly associated with AIS with an odds ratio of 0.8691 and 1.203, respectively. There was no significant association of rs1455114 with AIS. Moreover, AIS patients were found to have significantly increased expression of ABO. As for expression of CDH13 and SOX6, no remarkable difference was found between the two groups. CONCLUSION: The association of CDH13 and ABO variants with AIS was successfully replicated in the Chinese Han population. More studies are warranted to explore the functional role of ABO in the development of AIS. LEVEL OF EVIDENCE: N/A.


Assuntos
Sistema do Grupo Sanguíneo ABO/genética , Fatores de Transcrição SOXD/genética , Escoliose/epidemiologia , Escoliose/genética , Adolescente , Grupo com Ancestrais do Continente Asiático/genética , Caderinas , Feminino , Frequência do Gene , Estudos de Associação Genética , Predisposição Genética para Doença , Estudo de Associação Genômica Ampla , Genótipo , Humanos , Masculino , Razão de Chances , Polimorfismo de Nucleotídeo Único , Vigilância da População
16.
World Neurosurg ; 126: e901-e906, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30872192

RESUMO

OBJECTIVE: To investigate effectiveness of brace treatment in patients with adolescent idiopathic scoliosis with curve between 40° and 45° and to determine predictive factors associated with bracing outcome. METHODS: Bracing was used to treat 90 patients with curve >40 degrees. Factors including Risser sign, age, sex, curve pattern, curve magnitude, and initial curve correction were compared between patients with curve improvement and patients with curve progression. Logistic regression analysis was used to determine the independent predictors of curve progression. RESULTS: Curve was improved in 34 (37.8%) patients and stabilized in 12 (13.3%) patients. Remarkable curve progression >50 degrees was observed in 44 (48.9%) patients. Intergroup comparison showed significant differences between the 2 groups in terms of age (12.3 ± 1.4 years vs. 13.2 ± 1.6 years, P = 0.01), initial curve correction (2.2% ± 5.4% vs. 19.7% ± 12.2%, P < 0.001), and curve pattern (P = 0.03). Logistic regression analysis showed that initial curve correction of <10% (odds ratio = 12.82, P < 0.001) and Risser grade of 0 (odds ratio = 1.46, P = 0.04) were significant indicators of curve progression. CONCLUSIONS: Bracing may produce a favorable outcome in certain patients with curve between 40° and 45°. It should be cautiously used in this situation, as there was a higher probability of bracing failure. It is important to differentiate patients at high risk of curve progression at an early stage to avoid overtreatment.


Assuntos
Braquetes , Escoliose/terapia , Adolescente , Criança , Progressão da Doença , Feminino , Humanos , Masculino , Estudos Retrospectivos , Resultado do Tratamento
17.
World Neurosurg ; 127: e132-e136, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30862593

RESUMO

BACKGROUND: Previous studies showed that several variants located around the IRX family may have functional roles in the development of adolescent idiopathic scoliosis (AIS). However, there was lack of knowledge concerning the target gene of the region on 5p13.3 and the role of IRX genes in the etiology of AIS. This study aimed to validate the relationship between the IRX family and AIS in a large-scale general population and to further investigate the target gene of the region, which was associated with AIS. METHODS: SNP rs12517904 and rs117273909 were genotyped in 1323 patients and 1670 age-matched healthy controls. Paraspinal muscle was collected from 70 AIS patients and 20 congenital scoliosis patients. Student's t-test was used to compare the IRX1 expression between AIS patients and controls. The 1-way analysis of variance test was used to compare the expression of the IRX genes among different genotypes. RESULTS: For rs12517904, patients were found to have a significantly higher frequency of allele T than the controls (37.6% vs. 34.7%, P = 0.02). Allele T can significantly add to the risk of AIS with an odds ratio of 1.14. AIS patients were found to have significantly lower IRX1 expression than the controls. Patients with genotype TT were found to have significantly lower IRX1 expression than those with genotype GG. CONCLUSIONS: Our large-scale case control study validated that the IRX1 gene could be the disease-associated gene of AIS. The variant rs12517904 of the IRX1 gene is functionally associated with the development of AIS in the Chinese population. The role of IRX1 in the onset of AIS is worthy of further investigation.


Assuntos
Proteínas de Homeodomínio/genética , Polimorfismo de Nucleotídeo Único , Escoliose/genética , Fatores de Transcrição/genética , Adolescente , Alelos , Grupo com Ancestrais do Continente Asiático/genética , Estudos de Casos e Controles , Feminino , Frequência do Gene , Genótipo , Proteínas de Homeodomínio/análise , Humanos , Músculo Esquelético/química , Reação em Cadeia da Polimerase em Tempo Real , Escoliose/congênito , Escoliose/epidemiologia , Escoliose/metabolismo , Fatores de Transcrição/análise
18.
J Nanosci Nanotechnol ; 19(6): 3059-3078, 2019 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-30744732

RESUMO

The carbon dioxide (CO2) is notorious as the greenhouse gas, which could cause the global warming and climate change. Therefore, the reduction of the atmospheric CO2 emissions from power plants and other industrial facilities has become as an increasingly urgent concern. In the recent years, CO2 capture and storage technologies have received a worldwide attention. Adsorption is considered as one of the efficient options for CO2 capture because of its cost advantage, low energy requirement and extensive applicability over a relatively wide range of temperature and pressure. The metal organic frameworks (MOFs) show widely potential application prospects in CO2 capture and storage owing to their outstanding textural properties, such as the extraordinarily high specific surface area, tunable pore size, ultrahigh porosity (up to 90%), high crystallinity, adjustable internal surface properties, and controllable structure. Herein, the most important research progress of MOFs materials on the CO2 capture and storage in recent years has been comprehensively reviewed. The extraordinary characteristics and CO2 capture performance of Zeolitic Imidazolate Frameworks (ZIFs), Bio-metal organic frameworks (bio-MOFs), IL@MOFs and MOF-composite materials were highlighted. The promising strategies for improving the CO2 adsorption properties of MOFs materials, especially the low-pressure adsorption performance under actual flue gas conditions, are also carefully summarized. Besides, CO2 is considered as an abundant, nontoxic, nonflammable, and renewable C1 resource for the synthesis of useful chemicals and fuels. The potential routes for resource utilization of the captured CO2 are briefly proposed.

19.
Nanomaterials (Basel) ; 9(2)2019 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-30781430

RESUMO

Hierarchical nanostructures (HNs) are possibly endowed with novel properties due to their complex three-dimensional (3D) structures. Here, we provide a novel stepwise growth strategy of Coordination Complex Transformation-Assisted Growth for fabricating HNs. By using this, we prepare a new wurtzite ZnS HNs-hollow chestnut-like hierarchical microspheres (HCHMs), which are mesoporous hollow microspheres with single crystalline nanorods arrayed densely and radially from the centre. The HCHMs formation depends on the stepwise decomposition of the two Zn2+ complexes ([Zn(en)m(H2O)2(3-m)]2+ and [Zn(en)m(NH3)2(3-m)]2+, natural number m < 3). As the reaction proceeds, [Zn2+] has been distinctly reduced due to the transformation from [Zn(en)m(H2O)2(3-m)]2+ to [Zn(en)m(NH3)2(3-m)]2+ with a high stability constant, leading to a low crystal growth rate to obtain single crystalline nanorods. Additionally, the generated bubbles (CO2, NH3) acting as a template can induce the generation of hollow structure. The as-prepared ZnS HCHMs show an enhanced photocatalytic hydrogen evolution activity due to the single crystalline wurtzite phase and the high surface area contributed by the hollow hierarchical structures, as well as the mesoporosity. The versatility of the coordination complex transformation-assisted growth strategy will open up new possibilities for fabricating HNs, especially for those transition metal ions with excellent complex capabilities.

20.
Carbohydr Polym ; 208: 161-167, 2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-30658787

RESUMO

To evaluate the antioxidant activity of polysaccharides from corn silk in H2O2 induced oxidative damage in L6 skeletal muscle cells. A polysaccharide (CSP2) was isolated from corn silk. Structure analysis of CSP2 with 1D and 2D NMR indicated that CSP2 was composed of (1→6)-linked α-d-glucose. The substituent of the α-d-glucose is composed of (1→3)-linked α-l-arabinose, (1→4)-linked ß-d-galactose and (1→3,5)-linked ß-d-mannose, with ß-d-xylose and α-l-rhamnose as terminal unit. CSP2 had the potential to scavenge DPPH and hydroxyl radical and inhibit hemolysis in vitro. CSP2 was found to suppress oxidant stress by improving the enzyme activities of SOD, CAT, and GPX. Rodamine 123 staining results showed that the pretreatment of CSP2 prevented changes in the mitochondrial membrane potential and increased the fluorescence intensity in L6 cells in the presence of H2O2. These results suggested that CSP2 could be utilized as a potential antioxidant supplement to prevent oxidative stress.


Assuntos
Peróxido de Hidrogênio/farmacologia , Polissacarídeos/química , Polissacarídeos/farmacologia , Zea mays/química , Animais , Antioxidantes/metabolismo , Catalase/metabolismo , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Glutationa Peroxidase/metabolismo , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Ratos , Superóxido Dismutase/metabolismo
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