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1.
Food Chem ; 366: 130629, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34314933

RESUMO

Hydrophilic fluorescent silicon nanoparticles (SiNPs) with good pH stability, salt-tolerance and anti-photobleaching were for the first time prepared from hydrophobic 3-glycidoxypropyltrimethoxysilane. Employing SiNPs as the fluorescence probe, selective quantification of curcuminoids based on the quenching effect was realized with a linearity of 0.046-7.4 µg/mL and a limit of detection of 17.6 ng/mL. Moreover, in light of fluorescence redshift of SiNPs corresponding to the elevated concentration of curcuminoids, a fluorescence colorimetric method was established based on only one extra probe, i.e. herein SiNPs. Thus, semi-quantification of curcuminoids (0-14.7 µg/mL) was visualized from blue to yellow color. Both the developed quantitative and semi-quantitative probe were successfully applied to determine curcuminoids in various actual food samples. Furthermore, SiNPs possessed low cytotoxicity and succeeded in intracellular curcuminoids imaging. The proposed SiNPs could be a promising fluorescence probe for multiple applications.


Assuntos
Nanopartículas , Silício , Colorimetria , Diarileptanoides , Corantes Fluorescentes
2.
Int J Mol Sci ; 22(19)2021 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-34639080

RESUMO

The unconventional yeast Yarrowia lipolytica is extensively applied in bioproduction fields owing to its excellent metabolite and protein production ability. Nonetheless, utilization of this promising host is still restricted by the limited availability of precise and effective gene integration tools. In this study, a novel and efficient genetic tool was developed for targeted, repeated, and markerless gene integration based on Cre/lox site-specific recombination system. The developed tool required only a single selection marker and could completely excise the unnecessary sequences. A total of three plasmids were created and seven rounds of marker-free gene integration were examined in Y. lipolytica. All the integration efficiencies remained above 90%, and analysis of the protein production and growth characteristics of the engineered strains confirmed that genome modification via the novel genetic tool was feasible. Further work also confirmed that the genetic tool was effective for the integration of other genes, loci, and strains. Thus, this study significantly promotes the application of the Cre/lox system and presents a powerful tool for genome engineering in Y. lipolytica.

3.
J Neurosci ; 2021 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-34642213

RESUMO

Recognition memory provides the ability to distinguish familiar from novel objects and places and is important for recording and updating events to guide appropriate behaviour. The hippocampus (HPC) and medial prefrontal cortex (mPFC) have both been implicated in recognition memory, but the nature of HPC-mPFC interactions, and its impact on local circuits in mediating this process is not known. Here we show that novelty discrimination is accompanied with higher theta (4-10 Hz) activity and increased c-Fos expression in both these regions. Moreover, theta oscillations were highly coupled between the HPC and mPFC during recognition memory retrieval for novelty discrimination, with the HPC leading the mPFC, but not during initial learning. Principal neurons and interneurons in the mPFC responded more strongly during recognition memory retrieval compared to learning. Optogenetic silencing of HPC input to the mPFC disrupted coupled theta activity between these two structures, as well as the animals' (male Sprague-Dawley rats) ability to differentiate novel from familiar objects. These results reveal a key role of monosynaptic connections between the HPC and mPFC in novelty discrimination via theta coupling and identify neural populations that underlie this recognition memory-guided behaviour.Significant Statement:Many memory processes are highly dependent on the inter-regional communication between the HPC and mPFC via neural oscillations. However, how these two brain regions coordinate their oscillatory activity to engage local neural populations to mediate recognition memory for novelty discrimination is poorly understood. This study revealed that the HPC and mPFC theta oscillations and their temporal coupling is correlated with recognition memory-guided behaviour. During novel object recognition, the HPC drives mPFC interneurons to effectively reduce the activity of principal neurons. This study provides the first evidence for the requirement of the HPC-mPFC pathway to mediate recognition memory for novelty discrimination and describes a mechanism for how this memory is regulated.

4.
BMJ Open ; 11(10): e048221, 2021 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-34635516

RESUMO

OBJECTIVES: The measurement of visceral fat (VF) is clinically important for the identification of individuals at high risk of visceral obesity-related health conditions. Bioelectrical impedance analysis (BIA) is a widely available and frequently used body composition assessment method, but there have been few validation studies for the measurement of VF. This validation study investigated agreement between BIA and CT for the assessment of VF in adults. DESIGN: Cross-sectional study. SETTING: Between 2015 and 2016 in China. PARTICIPANTS: A total of 414 adults (119 men and 295 women) aged 40-82 years. PRIMARY AND SECONDARY OUTCOME MEASURES: CT-visceral fat area (VFA) was derived at the L2-3 and umbilicus level and VFA cut-offs for visceral obesity applied. BIA measurements of visceral fat level were compared with CT VFA findings using scatter plots and receiver operator characteristic (ROC) curves. RESULTS: Scatter plots showed poor agreement between BIA and CT-derived visceral fat measurements in both sexes (R=0.387-0.636). ROC curves gave optimum figures for sensitivity and specificity of 65% and 69% in women and 76% and 70% in men, respectively, for BIA to discriminate between adults with normal levels of VF and those with visceral obesity determined by CT. CONCLUSION: BIA has limited accuracy for the assessment of VF in adults in practice when compared with the criterion method.

5.
Ann Surg Oncol ; 2021 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-34637058

RESUMO

BACKGROUND: Patients with intermediate to advanced stage hepatocellular carcinoma (HCC; Barcelona Clinic Liver Cancer [BCLC] stage B/C) have few choices of curable treatments and thus suffer from dismal outcomes. Although surgical resection could prolong survival in certain selected patients with BCLC stage B/C HCC, the frequent postoperative recurrence and poor survival of these patients need to be improved by combining other therapies perioperatively. OBJECTIVE: This study was conducted to investigate the survival associations of adjuvant portal vein perfusion chemotherapy (PVC) and neoadjuvant hepatic arterial infusion chemotherapy (HAIC) in patients with resectable BCLC stage B/C HCC. METHODS: A retrospective study was conducted in consecutive patients who underwent R0 resection for intermediate to advanced stage HCC, combined with either PVC or HAIC perioperatively between January 2017 and December 2018. Patients treated with PVC or HAIC were analyzed according to intention-to-treat (ITT) and per protocol (PP) principles, respectively. The chemotherapy regimen of adjuvant PVC and neoadjuvant HAIC included 5-fluorouracil/leucovorin/oxaliplatin. Survival analysis and Cox regression for overall survival (OS) and event-free survival (EFS) were used to compare the outcomes. RESULTS: Among all 64 patients enrolled in this study, 28 received perioperative PVC and 36 received HAIC for ITT analysis. Age (median 44.00 vs. 46.50 years; p = 0.364), sex (male: 25/28 vs. 35/36; p = 0.435), and tumor size (median 9.55 vs. 8.10 cm; p = 0.178) were comparable between the two groups. In the ITT analysis, the median OS was significantly longer in patients in the HAIC group compared with the PVC group (median OS not reached vs. 19.47 months; p = 0.004); in the PP analysis, patients who received neoadjuvant HAIC followed by hepatectomy presented with much better EFS than patients in the PVC group (modified EFS 16.90 vs. 3.17 months; p = 0.022); and in the multivariate analysis, neoadjuvant HAIC presented as a significant predictor for enhanced EFS (hazard ratio [HR] 0.296; p = 0.007) and OS (HR 0.095; p = 0.007) for BCLC stage B/C HCC patients who received hepatectomy. CONCLUSIONS: Compared with adjuvant PVC, neoadjuvant HAIC treatment was associated with better survival and fewer recurrences in HCC patients who received R0 resection at the intermediate to advanced stage. These results need to be further validated prospectively.

6.
Cell Metab ; 2021 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-34619075

RESUMO

Over the last few decades, China has witnessed a great leap in economic growth and social welfare. Unfortunately, Chinese people have also been affected by a pandemic of over-nutrition, lack of physical activity, and increasing prevalence of metabolic disorders including obesity, diabetes, non-alcohol fatty liver disease, and cardiovascular disease. For instance, China currently has the largest number of diabetic patients (∼116 million) in the world. The fire of metabolic disorders is further fanned by the increased aging population, according to the survey results from the National Bureau of Statistics. On the other hand, progress in metabolic research has also made big strides. Here, we offer a glimpse at metabolic research in China, including not only its status quo but also its prospects, which aims to make significant contributions to our understanding of metabolism from bench to bedside.

7.
Blood Purif ; : 1-13, 2021 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-34610595

RESUMO

INTRODUCTION: The clinical effectiveness of AN69-oXiris remains unclear. This study aimed to compare the effects of AN69-oXiris and AN69-ST filters on cytokine levels and clinical improvement in septic patients. METHODS: This prospective observational study recruited septic patients who underwent blood purification in the First Affiliated Hospital of Soochow University between December 2019 and May 2020. Patients were assigned to an AN69-oXiris (oXiris) or AN69-ST (ST) group based on their preferred filter. Patients' clinical data, cytokine levels, and prognostic indicators were analysed at baseline (T0), 24 h after treatment (T1), and at the end of the treatment (T2). RESULTS: Forty-four patients participated in this study (22 patients in each group). Participants in both groups showed improvements in mean arterial pressure (MAP) values, oxygenation indices, and urinary output, and decreased vasoactive-inotropic scores (VISs), heart rates, lactic acid levels, and serum creatinine levels after blood purification. Reductions in cytokine levels were observed at T1 in both groups. Improvement the haemodynamic status was higher in the oXiris group than in the ST group at T2 (MAP: 79.0 [76.0, 85.0] vs. 77.0 [72.75, 79.25] mm Hg, p = 0.04; VISs: 9.10 [0.00, 16.69] vs. 19.05 [10.60, 26.33], p = 0.03, respectively). Patients in the oXiris group also had lower cytokine levels than those in the ST group at T1 (tumour necrosis factor-α: 24.55 [16.9, 30.15] vs. 30.15 [23.38, 34.13] pg/mL, p = 0.04; interleukin (IL)-6: 66.63 [46.21, 102.20] vs. 125.48 [79.73, 167.97] pg/mL, p = 0.01; IL-8: 53.59 [35.10, 66.01] vs. 63.60 [45.58, 83.37] pg/mL, p = 0.04; IL-10: 13.50 [10.35, 18.68] vs. 17.15 [13.80, 21.95] pg/mL, p = 0.04, respectively). There were no significant differences between the 2 groups regarding hospital mortality, intensive care unit length of stay (LOS), and hospital LOS. CONCLUSION: Blood purification using the AN69-oXiris or AN69-ST filter proved useful for septic patients, which was associated with reduced cytokine levels and improved clinical condition. Patients treated with AN69-oXiris had a more remarkable improvement in haemodynamic status and lower cytokine levels than those treated with AN69-ST filter, but there were no differences in clinical outcomes. Further investigations are needed to prove this finding.

9.
Appl Opt ; 60(24): 7136-7144, 2021 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-34612999

RESUMO

A wide-field imaging spectrometer based on a grating prism is proposed. The grating and prism parameters are discussed to balance spectral distortion over the entire band. The design method of the grating-prism (GP) dispersive module and the catadioptric optics of the spectrometer are discussed in detail. A high optical speed (F/2.4) and long slit (29.4 mm) visible and near-infrared design with high image quality and small distortion is presented. The results show that the optical performance of the GP imaging spectrometer is excellent. The tolerance analysis indicates that the GP spectrometer can be easily manufactured and implemented. The prototype has been tested in the laboratory and outdoors, and the results confirmed that the design will be useful in the fields of aeronautics and astronautics.

10.
Appl Opt ; 60(26): 8016-8021, 2021 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-34613062

RESUMO

A novel, to the best of our knowledge, method is proposed in this study to permit the controllable resolution of a micro-angle measurement by using a Michelson interferometer. The resolution of the proposed system can be adjusted by changing the distances between a pair of parallel mirrors. Through experiments, it was observed that as the distance was changed from 0 to 6 mm, the corresponding resolution was significantly altered from 22.88 to 14.02 µrad. Compared with other small angle measurement methods, the proposed method can realize the conversion of multiple measurement resolutions more easily and conveniently.

11.
Psychoneuroendocrinology ; 134: 105432, 2021 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-34607174

RESUMO

Adverse childhood experiences (ACEs) has been associated not only with an increased vulnerability for stress-related psychiatric disorders but also with distinct alterations of the hypothalamic-pituitary-adrenal (HPA) axis function and the immune system. The aim of this study is to examine differences in the HPA axis between major depressive disorder (MDD) patients with and without ACEs, and to explore differences in efficacy and HPA changes after long term antidepressant treatment between these two groups. A cohort of 803 patients with MDD were recruited. After the determination of cortisol (COR) and adrenocorticotropic hormone (ACTH), Hamilton Rating Scale for Depression (HAMD), Hamilton Rating Scale for Anxiety (HAMA), the Childhood Trauma Questionnaire (CTQ), 403 subjects were recruited for the following treatment study. Finally 330 MDD patients finished the monotherapy treatments of four antidepressants (Fluoxetine, Sertraline, Venlafaxine-extended release (XR), Duloxetine hydrochloride) for 12 weeks. Of 403 patients, 226 (56%) patients reported ACEs. Total score of HAMD in MDD with ACEs were higher than those in MDD without ACEs. There were significant differences for both ACTH and COR between MDD patients with and without ACEs that MDD patients with any types of maltreatment had higher level. Both COR and ACTH was positively and significantly correlated with the total scores of CTQ, HAMD, HAMA. After 12 weeks treatment of antidepressants monotherapy, the mean (SD) changes in HAMD and HAMA total scores was greater in MDD without ACEs than those in MDD with ACEs. At the 12-week end point, response was achieved by 37.2% in the MDD with ACEs group, 59.0% in the MDD without ACEs group respectively, with significant difference. Remission was achieved by 15.2% in the MDD with ACEs group and 32.2% in the MDD without ACEs group, with significant difference. The change in ACTH level in MDD without ACEs was also greater than that in MDD with ACEs, which was positively and significantly correlated with the HAMD total score only in MDD patient without ACEs. Logistic regression analysis showed that the total scores of CTQ, level of COR and ACTH at baseline were significantly associated with the response and remission. These findings indicated that exposure to ACEs for MDD could influence the HPA function and severity of symptoms. ACEs, ACTH and COR could be used as predictors of long term antidepressant treatment, suggested that are poor prognostic signs for antidepressants monotherapy in MDD with ACEs.

12.
Mol Med Rep ; 24(6)2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34664679

RESUMO

Cerebral vasospasm (CVS) is a common complication of subarachnoid hemorrhage (SAH) with high deformity rates and cerebral vascular smooth muscle cells (VSMCs) phenotypic switch is considered to be involved in the regulation of CVS. However, to the best of the authors' knowledge, its underlying molecular mechanism remains to be elucidated. Peroxisome proliferator­activated receptor ß/δ (PPARß/δ) has been demonstrated to be involved in the modulation of vascular cells proliferation and maintains the autoregulation function of blood vessels. The present study investigated the potential effect of PPARß/δ on CVS following SAH. A model of SAH was established by endovascular perforation on male adult Sprague­Dawley rats, and the adenovirus PPARß/δ (Ad­PPARß/δ) was injected via intracerebroventricular administration prior to SAH. The expression levels of phenotypic markers α­smooth muscle actin and embryonic smooth muscle myosin heavy chain were measured via western blotting or immunofluorescence staining. The basilar artery diameter and vessel wall thickness were evaluated under fluorescence microscopy. SAH grade, neurological scores, brain water content and brain swelling were measured to study the mechanisms of PPARß/δ on vascular smooth muscle phenotypic transformation. It was revealed that the expression levels of synthetic proteins were upregulated in rats with SAH and this was accompanied by CVS. Activation of PPARß/δ using Ad­PPARß/δ markedly upregulated the contractile proteins elevation, restrained the synthetic proteins expression and attenuated SAH­induced CVS by regulating the phenotypic switch in VSMCs at 72 h following SAH. Furthermore, the preliminary study demonstrated that PPARß/δ downregulated ERK activity and decreased the expression of phosphorylated (p­)ETS domain­containing protein Elk­1 and p­p90 ribosomal S6 kinase, which have been demonstrated to serve an important role in VSMC phenotypic change. Additionally, it was revealed that Ad­PPARß/δ could positively improve CVS by ameliorating the diameter of the basilar artery and mitigating the thickness of the vascular wall. Furthermore, subsequent experiments demonstrated that Ad­PPARß/δ markedly reduced the brain water content and brain swelling and improved the neurological outcome. Taken together, the present study identified PPARß/δ as a useful regulator for the VSMCs phenotypic switch and attenuating CVS following SAH, thereby providing novel insights into the therapeutic strategies of delayed cerebral ischemia.

13.
J Clin Oncol ; : JCO2100608, 2021 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-34648352

RESUMO

PURPOSE: In a previous phase II trial, hepatic arterial infusion chemotherapy (HAIC) with infusional fluorouracil, leucovorin, and oxaliplatin (FOLFOX) yielded higher treatment responses than transarterial chemoembolization (TACE) in large unresectable hepatocellular carcinoma. We aimed to compare the overall survival of patients treated with FOLFOX-HAIC versus TACE as first-line treatment in this population. METHODS: In this randomized, multicenter, open-label trial, adults with unresectable hepatocellular carcinoma (largest diameter ≥ 7 cm) without macrovascular invasion or extrahepatic spread were randomly assigned 1:1 to FOLFOX-HAIC (oxaliplatin 130 mg/m2, leucovorin 400 mg/m2, fluorouracil bolus 400 mg/m2 on day 1, and fluorouracil infusion 2,400 mg/m2 for 24 hours, once every 3 weeks) or TACE (epirubicin 50 mg, lobaplatin 50 mg, and lipiodol and polyvinyl alcohol particles). The primary end point was overall survival by intention-to-treat analysis. Safety was assessed in patients who received ≥ 1 cycle of study treatment. RESULTS: Between October 1, 2016, and November 23, 2018, 315 patients were randomly assigned to FOLFOX-HAIC (n = 159) or TACE (n = 156). The median overall survival in the FOLFOX-HAIC group was 23.1 months (95% CI, 18.5 to 27.7) versus 16.1 months (95% CI, 14.3 to 17.9) in the TACE group (hazard ratio, 0.58; 95% CI, 0.45 to 0.75; P < .001). The FOLFOX-HAIC group showed a higher response rate than the TACE group (73 [46%] v 28 [18%]; P < .001) and a longer median progression-free survival (9.6 [95% CI, 7.4 to 11.9] v 5.4 months [95% CI, 3.8 to 7.0], P < .001). The incidence of serious adverse events was higher in the TACE group than in the FOLFOX-HAIC group (30% v 19%, P = .03). Two deaths in the FOLFOX-HAIC group and two in the TACE group were deemed to be treatment-related. CONCLUSION: FOLFOX-HAIC significantly improved overall survival over TACE in patients with unresectable large hepatocellular carcinoma.

14.
Int Immunopharmacol ; 101(Pt A): 108169, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34607227

RESUMO

OBJECTIVE: This study was aimed to investigate the effect of monomer derivative of paeoniflorin (MDP) on macrophage pyroptosis mediated by TLR4/NLRP3/GSDMD signaling pathway in adjuvant arthritis (AA) rats. METHOD: Wistar rats were divided into normal group, AA model group, MDP (50 mg/kg) group and MTX (0.5 mg/kg) group. The expression of TLR4, NLRP3 and GSDMD in macrophage were detected by immunofluorescence assay. The expression of TLR4 and the ratio of macrophage pyroptosis were analyzed by flow cytometry. Cell morphology was observed by scanning electron microscopy. The cytokine levels of IL-18 and IL-1ß were detected by ELISA. The expressions of proteins related to macrophage pyroptosis were detected by western blot. RESULTS: MDP has a therapeutic effect on rats AA by reducing the secondary inflammation and improving pathological changes. The results of X-ray imaging and ultrasound images showed that MDP could inhibit bone erosion, soft tissue swelling, and joint space narrowing. Macrophage pyroptosis was found in secondary inflammation of AA rats. The expressions of TLR4, MyD88, NLRP3, Caspase-1, ASC, and GSDMD-N in macrophage were increased, the levels of IL-18 and IL-1ß were enhanced, and the morphology of pyroptosis could be observed. MDP could inhibit macrophage polarization and macrophage pyroptosis, and down-regulated the cytokine levels of IL-18 and IL-1ß in AA rats. MDP could regulate the M1/M2 ratio, decreased the ratio of macrophage pyroptosis and down-regulated the expressions of TLR4, MyD88, NLRP3, Caspase-1, ASC, and GSDMD-N in vivo and in vitro. CONCLUSION: Abnormal activation of TLR4/NLRP3/GSDMD signaling pathway may be involved in macrophage pyroptosis in AA rats. The therapeutic effect of MDP on AA rats is related to the inhibition of macrophage pyroptosis by regulating the TLR4/NLRP3/GSDMD signaling pathway.

15.
Chin J Dent Res ; 24(3): 191-198, 2021 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-34491014

RESUMO

OBJECTIVE: To investigate various factors affecting the clinical outcome of nonsurgical periodontal treatment and evaluate the treatment effects of adjunctive amoxicillin and metronidazole (AMX + MET) in patients with generalised aggressive periodontitis (GAgP). METHODS: Forty-two patients with GAgP were recruited and randomly assigned to three groups: scaling and root planing (SRP) only, AMX + MET after SRP, and AMX + MET during SRP. The patients were assessed every 2 months post-therapy. Periodontal clinical and subgingival microbiological parameters were analysed at baseline and 6 months post-therapy. The impacts of different covariates on pocket probing depth (PD) reduction were evaluated. RESULTS: A multilevel analysis revealed that 58% of the variability in PD reduction was attributed to site-level parameters, 27.3% to patient-level parameters and 18.7% to tooth-level parameters. Greater PD reduction can be expected at initially deeper PD sites and sites with intrabony defects, and in patients with adjunctive use of AMX + MET. Persistent Tannerella forsythia infection and tooth mobility after treatment were negatively associated with PD reduction. CONCLUSION: The clinical outcomes of nonsurgical periodontal treatment were mainly influenced by site-level parameters, and adjunctive use of AMX + MET can lead to better clinical results in patients with GAgP in a short time.


Assuntos
Periodontite Agressiva , Periodontite Agressiva/terapia , Amoxicilina/uso terapêutico , Antibacterianos/uso terapêutico , Raspagem Dentária , Humanos , Análise Multinível , Aplainamento Radicular , Resultado do Tratamento
16.
PLoS One ; 16(9): e0254627, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34492027

RESUMO

We used fresh leaves of Sophora japonica L. variety 'Qingyun 1' (A0) and 10 superior clones of the same species (A1-A10) to explore leaf morphological characteristics and total particle retention per unit leaf area under natural and artificial simulated dust deposition treatments. Our objectives were to explore the relationship between the two methods and to assess particle size distribution, X-ray fluorescence (XRF) heavy metal content, and scanning electron and atomic force microscopy (SEM and AFM) characteristics of leaf surface microstructure. Using the membership function method, we evaluated the dust retention capacity of each clone based on the mean degree of membership of its dust retention index. Using correlation analysis, we selected leaf morphological and SEM and AFM indices related significantly to dust retention capacity. Sophora japonica showed excellent overall dust retention capacity, although this capacity differed among clones. A5 had the strongest overall retention capacity, A2 had the strongest retention capacity for PM2.5, A9 had the strongest retention capacity for PM2.5-10, A0 had the strongest retention capacity for PM>10, and A2 had the strongest specific surface area (SSA) and heavy metal adsorption capacity. Overall, A1 had the strongest comprehensive dust retention ability, A5 was intermediate, and A7 had the weakest capacity. Certain leaf morphological and SEM and AFM characteristic indices correlated significantly with the dust retention capacity.

17.
Ann Palliat Med ; 10(8): 9136-9148, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34488399

RESUMO

BACKGROUND: As the aging population continues to increase worldwide, the prevalence of cardiovascular diseases and muscular dystrophy/sarcopenia in the elderly has escalated significantly. Cardiovascular diseases elevate the risk of muscular atrophy/sarcopenia, which results in increased disability and mortality of patients. This study analyzed the current available literature related to the relationship between cardiovascular diseases and muscular atrophy/sarcopenia in the aging population. METHODS: The Science Citation Index Expanded (SCI-E) database was searched for related literature published between 1900 and March 14, 2021. The subject search was performed using the search terms "muscular atrophy" and "sarcopenia". The search formula was "muscular atrophy OR sarcopenia". The search scope was limited to "cardiovascular diseases OR cardiac & cardiovascular systems". All search results and cited references were exported in plain text format and Citespace software was used to analyze the publications in terms of year of publication, country and institution, journal of publication, authors, and keywords. RESULTS: A total of 1,004 related research documents were obtained, with a citation frequency of 26,705 times. The top five countries for the highest number of published documents were the United States, Japan, Germany, South Korea, and Italy. The top five countries involved in research cooperation were the United States, Japan, the United Kingdom, Spain, and Germany, however, overall, there was little cooperation between countries, institutions, and authors. A number of researchers from Germany published the most documents. The author with the most cited publication was Cruz-Jentoft et al. from Spain, which deserves special attention. Professional journals of in the field of geriatrics play a significant role in this research topic. Analysis of the keywords showed that current researchers are mainly concerned with the associated risk of death. CONCLUSIONS: The relationship between muscular atrophy/sarcopenia and cardiovascular diseases is currently a hot topic of research in geriatrics and cardiovascular disease, and further studies examining the mechanisms involved and potential prevention strategies are warranted.


Assuntos
Doenças Cardiovasculares , Sarcopenia , Idoso , Bibliometria , Alemanha , Humanos , Reino Unido , Estados Unidos
18.
Chemosphere ; 287(Pt 1): 131884, 2021 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-34474380

RESUMO

The disposing of municipal solid waste incineration(MSWI) fly ashes containing dioxins is an intractable problems. Co-sintering is one of the most ideal methods to dispose MSWI fly ash, because it not only degrades the dioxins but also makes it possible to re-utilize MSWI fly ashes. In the present study, MSWI fly ash(FA) and water washed MSWI fly ash(WFA) were added into the sinter raw mixture in a lab-scale sintering pot. Different effects of fly ash and water washed fly ash on emission characteristics of dioxins were studied, and possible pathways to form dioxins were discussed in detail. During co-sintering, at least 88.9%, 99.1% of dioxins brought in by FA, WFA was decomposed, respectively, and re-synthesis with a significant distribution characteristic of dioxins originated from sintering process. In the preheating and dry zone, the recombination and condensation reaction of precursors were the main formation pathways of 2,3,7,8-PCDDs in the sintering process and 2,3,7,8-PCDFs were formed by de novo synthesis. In addition, the resynthesis process was affected by chlorine and calcium brought in by fly ash. Sufficient chlorine boosted the chlorination of reactants while calcium increased the residence time, causing the emission concentration of toxic dioxins and the adding ratio were nonlinearly dependent. Therefore, the maximum proportion of water washed MSWI fly ash that can be added into the sintering process was 0.5 wt.%. Besides, the possible pathways to form dioxins were proposed.

19.
J Immunol Res ; 2021: 7183136, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34485536

RESUMO

The intestinal mucosal immune barrier protects the host from the invasion of foreign pathogenic microorganisms. Immune cells and cytokines in the intestinal mucosa maintain local and systemic homeostasis by participating in natural and adaptive immunity. Deficiency of the intestinal mucosal immune barrier is associated with a variety of intestinal illnesses. Exosomes are phospholipid bilayer nanovesicles that allow cell-cell communication by secreting physiologically active substances including proteins, lipids, transcription factors, mRNAs, micro-RNAs (miRNAs), and long noncoding RNAs (lncRNAs). Exosomal lncRNAs are involved in immune cell differentiation and the modulation of the immune response. This review briefly introduces the potential role of exosomal lncRNAs in the intestinal mucosal immune barrier and discusses their relevance to intestinal illnesses.

20.
Eur Radiol ; 2021 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-34482428

RESUMO

OBJECTIVE: To develop and validate deep learning (DL) methods for diagnosing autism spectrum disorder (ASD) based on conventional MRI (cMRI) and apparent diffusion coefficient (ADC) images. METHODS: A total of 151 ASD children and 151 age-matched typically developing (TD) controls were included in this study. The data from these subjects were assigned to training and validation datasets. An additional 20 ASD children and 25 TD controls were acquired, whose data were utilized in an independent test set. All subjects underwent cMRI and diffusion-weighted imaging examination of the brain. We developed a series of DL models to separate ASD from TD based on the cMRI and ADC data. The seven models used include five single-sequence models (SSMs), one dominant-sequence model (DSM), and one all-sequence model (ASM). To enhance the feature detection of the models, we embed an attention mechanism module. RESULTS: The highest AUC (0.824 ~ 0.850) was achieved when applying the SSM based on either FLAIR or ADC to the validation and independent test sets. A DSM using the combination of FLAIR and ADC showed an improved AUC in the validation (0.873) and independent test sets (0.876). The ASM also showed better diagnostic value in the validation (AUC = 0.838) and independent test sets (AUC = 0.836) compared to the SSMs. Among the models with attention mechanism, the DSM achieved the highest diagnostic performance with an AUC, accuracy, sensitivity, and specificity of 0.898, 84.4%, 85.0%, and 84.0% respectively. CONCLUSIONS: This study established the potential of DL models to distinguish ASD cases from TD controls based on cMRI and ADC images. KEY POINTS: • Deep learning models based on conventional MRI and ADC can be used to diagnose ASD. • The model (DSM) based on the FLAIR and ADC sequence achieved the best diagnostic performance with an AUC of 0.836 in the independent test sets. • The attention mechanism further improved the diagnostic performance of the models.

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