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1.
Stem Cell Res Ther ; 12(1): 226, 2021 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-33823915

RESUMO

BACKGROUND: Wound healing is impaired in patients with diabetes due to the multifactorial etiology of the disease, which limits the therapeutic efficacy of various approaches. This study hypothesizes that the combination of adipose-derived stem cells (ADSCs) and platelet-rich plasma (PRP) might achieve optimally efficient diabetic wound healing. METHODS: ADSCs were isolated from the adipose tissues of Sprague-Dawley (SD) rats. PRP was prepared by using a two-step centrifugation technique. A diabetic wound model was established on the backs of SD rats to evaluate the effect of ADSCs incorporated into PRP. Hematoxylin and eosin staining, immunofluorescence, and immunohistochemistry were performed to observe the changes in neovascularization. ELISA and Western blot were utilized to detect the angiogenesis-related protein expression levels. The proliferation of endothelial cells was assessed by the MTS assay. RESULTS: ADSCs incorporated into PRP induced a higher wound closure rate than ADSCs, PRP, and negative control. The expression levels of VEGF, p-STAT3, and SDF-1 in the ADSC+PRP group were higher than those in the other groups. Moreover, the proliferation of endothelial cells was strongly stimulated by treatment with the combination of ADSC-conditioned medium (ADSC-CM) and PRP. CONCLUSIONS: PRP enhanced diabetic wound healing induced by ADSCs, and its promoting effect involved neovascularization.

2.
Eur J Pharmacol ; 900: 174080, 2021 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-33811839

RESUMO

Treatment of visceral pain originating from the uterine cervix is a substantial clinical problem. The underlying mechanisms of such visceral pain remain unclear mainly due to a lack of reliable model. This study aimed to develop and evaluate the performance of a rat model of pain induced by uterine cervix inflammation. Rats were randomized to six groups according to the solution injected into the uterine cervix: normal saline, vehicle, capsaicin (0.3 mg, 0.6 mg, 0.9 mg), capsaicin 0.9 mg + morphine (n = 15 in each group). Spontaneous behaviors after cervical injection were recorded by a computerized video system and analyzed offline. An equation for calculating a novel pain score was derived from particular behaviors, based on Pearson's correlation analysis and regression analysis. c-Fos expression in the spinal cord was detected. The pain score and c-fos expression in the spinal cord were highest in the 0.9 mg capsaicin group and lowest in the normal saline and vehicle groups (P < 0.05). Intrathecal morphine significantly decreased the pain score (P < 0.05) and c-fos expression in the spinal cord (P < 0.05). Injection of capsaicin into the uterine cervix in rats could be a practical model of inflammatory cervical pain, which can be evaluated using our novel pain score. This model will provide further insight into the mechanism underlying visceral pain originating from the uterine cervix.

3.
Hemoglobin ; : 1-4, 2021 Mar 29.
Artigo em Inglês | MEDLINE | ID: mdl-33775194

RESUMO

We describe a new α-globin chain variant in a Chinese subject. This novel variant, with a Val→Met substitution at codon 93 of the α-globin chain, has been named Hb Qingcheng (HBA1: c.280G>A) for where the proband was born. A woman with somatic mosaicism for Hb Qingcheng presented with the phenotype of mild α-thalassemia (α-thal).

4.
Ann Palliat Med ; 10(3): 2880-2888, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33691457

RESUMO

BACKGROUND: Despite the association of masticatory function with head posture and mandible movement, the impact of head posture on masseter muscle electrical activity remains unclear. This study aimed to investigate the electrical activity of the anterior temporal, masseter, superior trapezius, and sternocleidomastoid muscles in temporomandibular disorder (TMD) patients in three different head positions and compare the findings with those for healthy subjects. METHODS: The study included 16 TMD patients and 17 healthy subjects. Surface electromyography (sEMG) was used to assess the bilateral anterior temporalis, masseter, superior trapezius, and sternocleidomastoid muscle activities. The maximal voluntary contraction (MVC) and root mean square (RMS) sequence of each muscle were measured in three head positions. Mixed analysis of variance was used to evaluate the effects of head posture on muscle electrical activity in each group. RESULTS: The group differences for the bilateral masseter muscles were significant in the relaxation position, neutral head position (NHP), and relaxation position with tooth contact (P<0.05). In both groups, the basal RMSs of the bilateral anterior temporalis muscle in the relaxation position with tooth contact was significantly higher than that in the NHP (P<0.05). In both groups, the basal RMSs of the bilateral masseter muscles in the relaxation position with tooth contact was higher than that in the relaxation position and NHP, with significant differences (P<0.05). CONCLUSIONS: During the management of TMD patients, physical therapists should focus on patient education regarding maintenance of a relaxed position and establish appropriate rehabilitation programs to reduce muscle activity.

5.
Neuroscience ; 461: 91-101, 2021 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-33722672

RESUMO

Caveolin-1 (Cav-1) is a constitutive structural protein of caveolae in the plasma membrane. It plays an important role in maintaining blood brain barrier (BBB) integrity. In this study, we identified that miR-103-3p, a hypoxia-responsive miRNA, could interact with Cav-1. In endothelial cells, miR-103-3p mimic diminished the expression of Cav-1 and tight junction proteins, which were rescued by the inhibition of miR-103-3p. We found a substantial increase of miR-103-3p and decease of Cav-1 in the rat subarachnoid hemorrhage (SAH) model. Pre-SAH intracerebroventricularly injection of miR-103-3p antagomir relieved Cav-1 loss, sequentially reduced BBB permeability and improved neurological function. Finally, we demonstrated that the salutary effects of miR-103-3p antagomir were abolished in Cav-1 knock-out mice, suggesting that Cav-1 was required for the miR-103-3p inhibition-induced neurovascular protection. Taken together, our findings suggest that the inhibition of miR-103-3p could exert neuroprotective effects through preservation of Cav-1 and BBB integrity, making miR-103-3p a novel therapeutic target for SAH.

6.
Int J Rheum Dis ; 24(4): 599-607, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33650318

RESUMO

BACKGROUND: MicroRNAs (miRNAs) have been shown to play a crucial role in inflammation regulation; however, their relationship with inflammation in acute gouty arthritis has not been fully elucidated. Herein, we conducted a study to explore the regulatory roles of miR-223-3p and miR-22-3p in gouty-associated inflammation. METHODS: In vitro and in vivo experiments were conducted to examine the molecular mechanisms of miRNA regulation in gouty inflammation. Dual-luciferase reporter assay was used to verify the direct target of miR-223-3p and miR-22-3p. RESULTS: We found that miR-223-3p and miR-22-3p interacted with the 3' untranslated region segment of NLRP3 (nucleotide-binding domain leucine-rich repeat [NLR] and pyrin domain containing receptor 3) and inhibited its expression. A decreased expression of miR-223-3p and miR-22-3p was observed in both mice air pouch synovium and phorbol myristrate acetate-treated THP-1 cells stimulated with monosodium urate (P < .05). Compared with the negative control group, NLRP3 expression at the transcript and protein level in miR-223-3p and miR-22-3p overexpression group significantly decreased after 6 hours of monosodium urate treatment in vivo and in vitro (P < .05). The results of the dual-luciferase reporter assay demonstrated that miR-223-3p and miR-22-3p directly targeted NLRP3. CONCLUSION: The findings of the present study show that miR-223-3p and miR-22-3p can reduce the inflammatory effects of gout by inhibiting the expression of NLRP3.

7.
Nat Prod Res ; : 1-7, 2021 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-33771063

RESUMO

Two new lanostane-type triterpenoids, ganoderenicfys A (1) and B (2), together with six related known terpenoids (3-8), were isolated and identified from the fruiting body of Ganoderma applanatum. The structures of these compounds were established on the basis of detailed interpretation of their NMR and HRESIMS data. The absolute configurations of 1 and 2 were determined by quantum chemical electronic circular dichroism (ECD) calculations. All of the isolated compounds were evaluated for their proangiogenic activities in a transgenic fluorescent zebrafish model. Compounds 1-6 displayed dose-dependently proangiogenic activity in a PTK787-induced vascular injury zebrafish model, while compounds 1, 2 and 4 significantly promoted the angiogenesis. This is the first report for proangiogenic activities of lanostane-type triterpenoids.

9.
Plant Dis ; 2021 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-33754858

RESUMO

Cyclobalanopsis glauca (Thunb.) Oerst. is one of the most widely distributed species of evergreen broad-leaved tree in subtropical areas of China. It is also grown in Korea, Japan, and India. Because of its beautiful shape, C. glauca is commonly used for greening gardens and walkways. In July 2018, leaf spots on C. glauca were observed in Zhejiang province (Lishui, N: 28°26' 6.75";E: 119°54'11.22), China. About 70% of the trees were found to be diseased, with approximately 50% of leaves showing symptoms. The symptoms on C. glauca leaves initially appeared as small brown-yellow spots which gradually expanded, developing a light brown central and dark brown to black margin. The spots ranged from 4 to 15 mm in diameter. Ten symptomatic fragments measuring approximately 5×5 mm from each leaf were surface disinfested with 70% ethanol for 30 s, and then they were rinsed in sterile distilled water and placed on potato dextrose agar (PDA) medium at 25 °C in the dark for five days. Segments of colony perimeters were then transferred to new plates. The colonies initially produced white mycelia that later turned gray-white with pink and occasionally black dots scattered on the surface of the mycelium. Spores were aseptate, cylindrical, 8 to 15 µm in length, and 3 to 5 µm wide, most with rounded ends, a few with one apex round and the other fusiform, as described for Colletotrichum gloeosporioides (Penz.) Sacc. (Agostini et al. 1993). The internal transcribed spacer rDNA (ITS: MK758005) and two nuclear protein-coding genes (CHS: MK784770, ACT: MK784769) were amplified with ITS1/ITS4, CHS-79F/CHS-345, and ACT-512F/ACT-783R, respectively (Weir et al. 2012). The sequence had 99.61% identity to GQ485605 for ITS, 99.56% to GQ856782 for ACT, and 100% to GQ856733 for CHS of C. gloeosporioides CBS 953.97 in GenBank, respectively. To fulfill Koch's postulates, spores (1×108) of the isolate were sprayed onto leaves of twelve 2-year-old C. glauca plants (at least six leaves per plant). The fungus was inoculated on one side of each leaf, and distilled water was used as a mock inoculated control on the other side. The plants were cultivated in the greenhouse to maintain high humidity and a temperature near 25 °C. After 9 days, 100% of the leaf halves that had been inoculated had symptoms identical to those observed on affected C. glauca leaves in the field, while no symptoms were observed on the mock inoculated half of each leaf. The fungus was reisolated from the symptoms and identified as C. gloeosporioides using techniques previously described. To our knowledge, this is the first report of C. gloeosporioides infecting C. glauca in China. This study will establish a foundation for the further study of C. gloeosporioides to address the disease effectively. References: Agostini, J. P., et al. 1993. Phytopathology. 82:1177. Weir, B. S., et al. 2012. Stud. Mycol. 73:115. This work was supported by the National Science Foundation for Young Scientists of China (31800035).

10.
Oncol Rep ; 45(3): 1033-1043, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33650658

RESUMO

Bladder cancer is a common tumor type of the urinary system, which has high levels of morbidity and mortality. The first­line treatment is cisplatin­based combination chemotherapy, but a significant proportion of patients relapse due to the development of drug resistance. Therapy­induced senescence can act as a 'back­up' response to chemotherapy in cancer types that are resistant to apoptosis­based anticancer therapies. The circadian clock serves an important role in drug resistance and cellular senescence. The aim of the present study was to investigate the regulatory effect of the circadian clock on paclitaxel (PTX)­induced senescence in cisplatin­resistant bladder cancer cells. Cisplatin­resistant bladder cancer cells were established via long­term cisplatin incubation. PTX induced apparent senescence in bladder cancer cells as demonstrated via SA­ß­Gal staining, but this was not observed in the cisplatin­resistant cells. The cisplatin­resistant cells entered into a quiescent state with prolonged circadian rhythm under acute PTX stress. It was identified that the circadian protein cryptochrome1 (CRY1) accumulated in these quiescent cisplatin­resistant cells, and that CRY1 knockdown restored PTX­induced senescence. Mechanistically, CRY1 promoted p53 degradation via increasing the binding of p53 with its ubiquitin E3 ligase MDM2 proto­oncogene. These data suggested that the accumulated CRY1 in cisplatin­resistant cells could prevent PTX­induced senescence by promoting p53 degradation.

11.
Front Cell Infect Microbiol ; 11: 608352, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33680989

RESUMO

Listeriosis, caused by Listeria monocytogenes, is a severe food-borne infection. The nationwide surveillance in China concerning listeriosis is urgently needed. In the present study, 144 L. monocytogenes isolates were collected from the samples of blood, cerebrospinal fluid (CSF), and fetal membrane/placenta in China for 12 years from 2008 to 2019. We summarized these listeriosis patients' demographical and clinical features and outcomes. The susceptibility profile for 12 antibiotics was also determined by the broth microdilution method. Multilocus sequence typing (MLST) and serogroups of these listeria isolates were analyzed to designate epidemiological types. We enrolled 144 cases from 29 healthcare centers, including 96 maternal-neonatal infections, 33 cases of bacteremia, 13 cases of neurolisteriosis, and two cutaneous listeriosis. There were 31 (59.6%) fetal loss in 52 pregnant women and four (9.8%) neonatal death in 41 newborns. Among the 48 nonmaternal-neonatal cases, 12.5% (6/48) died, 41.7% (20/48) were female, and 64.6% (31/48) occurred in those with significant comorbidities. By MLST, the strains were distinguished into 23 individual sequence types (STs). The most prevalent ST was ST87 (49 isolates, 34.0%), followed by ST1 (18, 12.5%), ST8 (10, 6.9%), ST619 (9, 6.3%), ST7 (7, 4.9%) and ST3 (7, 4.9%). Furthermore, all L. monocytogenes isolates were uniformly susceptible to penicillin, ampicillin, and meropenem. In summary, our study highlights a high genotypic diversity of L. monocytogenes strains causing clinical listeriosis in China. Furthermore, a high prevalence of ST87 and ST1 in the listeriosis should be noted.

12.
Prev Med ; 146: 106484, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33647350

RESUMO

Since licensure of human papillomavirus (HPV) vaccine in mainland China, little research has been conducted about healthcare providers' (HCPs) understanding and recommendation of HPV vaccine. A multi-stage convenience sample of Chinese HCPs (N = 5270) were surveyed, involving obstetrician-gynecologists, HCPs from Division of Expanded Program on Immunization (DEPI), Community Health Center (CHC) and other non-HPV closely related professions. Binary logistic regression was conducted to explore factors associated with knowledge and recommendation behaviors. Overall, HCPs showed basic HPV/HPV vaccine knowledge with median (interquartile range) score at 9.5 (7.5-11.6) out of 16 and relatively high recommendation behavior (74.8%). Identified knowledge gaps among HCPs included risk factors of HPV infection, best time to vaccinate, prophylactic functions of HPV vaccine and especially classification of low-risk and high-risk types. Profession-specific analysis in individual knowledge item showed HCPs from CHC were suboptimal on HPV while obstetrician-gynecologists were less competent on HPV vaccine knowledge. Obstetrician-gynecologists also recommended vaccination less frequently than HCPs from DEPI and CHC. Besides being key predictors of recommendation practice (2.74, 95% CI: 2.34-3.21), knowledge shared independent determinants with recommendation behavior on age and ethnicity and additionally associated with education and title by itself. Findings highlight overall and profession-specific gaps on HPV and HPV vaccine knowledge and recommendation practice. Future education and training efforts should be profession-niche-targeting and focus much on HCPs with lower title or education background and from minorities.

13.
Environ Pollut ; 278: 116874, 2021 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-33752136

RESUMO

Plastics are globally distributed in oceans and can pose a threat to the environment and organisms. In this study, plastic pollution in surface water and sediments of the Bohai Sea was assessed based on plastic abundance, distribution and characteristics (shape, polymer, size and color). Water and sediment samples were collected across the sea using a plankton net (330 µm) and a grab sampler, respectively. The following conclusions were reached. 1) In surface water, large plastics were less abundant (0.14 items/m3) and showed less diverse characteristics than microplastics (0.79 items/m3) but did not significantly differ in spatial distribution. 2) Microplastics in water were more abundant (1.95 items/m3) with more diverse characteristics in Liaodong Bay than in other regions of the sea (0.26-0.59 items/m3). Plastic waste from highly concentrated agricultural, industrial and fishery activities could make large contributions to microplastics in Liaodong Bay. Additionally, low hydrodynamics and long distance to Bohai Strait are unfavorable for diffusion of particles, facilitating the retention of microplastics and increasing the abundance in this bay. 3) Microplastics in sediments were smaller in terms of dominant sizes (<0.5 mm) with less diverse characteristics than particles in water (0.5-1.5 mm). Specifically, fragments, foams and lines dominated among the microplastics in water, whereas fibers and fragments were dominant particles in sediments; alkyd resin, polyethylene, polystyrene and polypropylene (PP) predominated among the particles in water, but rayon, cellulose and PP were dominant particles in sediments. 4) Neither abundance nor size of microplastics in the two media was proportionally correlated and showed low similarity indexes of polymer (0.16), shape (0.29) or color (0.38). This could be related to mismatch in spatiotemporal distributions and variations in the characteristics, fate and behavior of microplastics in the two media. The findings provide knowledge for tracing the sources of plastics in the Bohai Sea.

14.
Molecules ; 26(3)2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33535547

RESUMO

It is counterintuitive that chemical reactions can be accelerated by freezing, but this amazing phenomenon was discovered as early as the 1960s. In frozen systems, the increase in reaction rate is caused by various mechanisms and the freeze concentration effect is the main reason for the observed acceleration. Some accelerated reactions have great application value in the chemistry synthesis and environmental fields; at the same time, certain reactions accelerated at low temperature during the storage of food, medicine, and biological products should cause concern. The study of reactions accelerated by freezing will overturn common sense and provide a new strategy for researchers in the chemistry field. In this review, we mainly introduce various mechanisms for accelerating reactions induced by freezing and summarize a variety of accelerated cryochemical reactions and their applications.

15.
Artigo em Inglês | MEDLINE | ID: mdl-33541086

RESUMO

Li-CO2 batteries have attracted worldwide attention because of their dual characteristics of high energy density and effective CO2 capture. However, the basic electrochemistry mechanism involved has been unclear, which is mainly confused by the complicated decomposition of organic electrolytes. Herein, water-in-salt (WIS, LiTFSI/H2O 21.0 mol/1 kg) has been explored as a suitable electrolyte for the first time to investigate the reaction mechanism of Li-CO2 batteries with different cathodes (carbon nanotube (CNT) and Mo2C/CNT, respectively). An Mo2C-based Li-CO2 battery with WIS delivers a higher energy efficiency of 83% and a superior cyclability, compared to those of the CNT-based counterpart cell. Through various ex/in situ qualitative/quantitative characterizations, the Mo2C-based Li-CO2 battery with WIS can operate on the reversible conversion of CO2-to-Li2C2O4 ((e-/CO2)ideal = 1) at lower discharge/charge overpotentials, while the CNT-based counterpart cell is based on the formation/decomposition of Li2CO3 ((e-/CO2)ideal ≈ 1.33) at high overpotentials. Such a difference in CO2 reduction products stems from the stronger interaction between Mo2C(101) and Li2C2O4 than that of the CNT and Li2C2O4 based on the density functional theory calculations, resulting in the selective stabilization of the intermediate product Li2C2O4 on the Mo2C surface.

16.
Biomed Res Int ; 2021: 6666680, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33564681

RESUMO

Patient education is important in the treatment of temporomandibular disorder (TMD), but little is known about its effect on oral behaviors. We aimed to determine the dominant oral behaviours in patients with TMD and assess the impact of education on such behaviours. Between July 2018 and April 2019, 54 patients diagnosed with TMD according to DC/TMD were recruited. They received physical therapy and were provided education on TMD and offered a list of recommendations for improving their oral behaviours. The patient education process usually lasted for 10-20 min. Of these patients, 48 were reexamined at the outpatient clinic, 3-9 months posttreatment. We recorded the Oral Behaviour Checklist (OBC) score, maximum painless mouth opening (mm), visual analogue scale (VAS) score for pain, and Jaw Functional Limitation Scale (JFLS) score pre- and posttreatment. Wilcoxon signed rank test and paired sample t-test were used for statistical analysis. Results showed that the most dominant oral behaviours included "putting pressure on the jaw" (59.3%); "chewing food on one side" (46.3%); "pressing, touching, or holding teeth together at times other than eating" (33.3%); and "eating between meals" (33.3%). Posttreatment, the patients reported a decrease in "chewing gum" (P = 0.002), "leaning with the hand on the jaw" (P = 0.013), "chewing food on one side" (P ≤ 0.001), and "eating between meals" (P = 0.007), but this change was not significant in subgroups with a follow-up interval of 9 months. We also observed a significant improvement in the maximum painless mouth opening (P ≤ 0.001), JFLS score (P ≤ 0.001), and VAS score (P ≤ 0.001) for pain, posttreatment. In conclusion, patient education can facilitate management of oral behaviours and should be targeted towards specific oral behaviours.

17.
Aging (Albany NY) ; 13(4): 5804-5823, 2021 02 17.
Artigo em Inglês | MEDLINE | ID: mdl-33601337

RESUMO

OBJECTIVES: Joint capsule fibrosis caused by excessive inflammation leading to post-traumatic joint contracture (PTJC). Fibroblasts trigger inflammation under the challenge of various proinflammatory cytokines. Macrophage migration inhibitory factor (MIF) is a prominent proinflammatory cytokine involved in inflammation- and fibrosis-associated pathophysiology, we investigated the role of MIF in PTJC. METHODS: Using rat PTJC model and fibroblast inflammation model, we detected MIF expression in posterior joint capsule. Primary joint capsule fibroblasts (JFs) were used to investigate the effects of MIF on cell proliferation, migration and proinflammatory cytokines production. The mechanism of JF-mediated events was evaluated by qRT-PCR, western blot and immunoprecipitation. We screened the mRNA expression profile to identify gene candidates that mediate the effect of MIF on JFs. RESULTS: MIF increased in posterior joint capsule following PTJC and co-localized with fibroblasts. Injection of MIF inhibitor significantly suppressed joint capsule inflammation and fibrosis. In vitro, MIF promoted JF proliferation, migration, and inflammation by regulating mitogen-activated protein kinase/nuclear factor-κB pathway through coupling with CD74. Transcriptome analysis revealed that lipid metabolism-related factors Pla2g2a, Angptl4, and Sgpp2, downstream of MIF/CD74, were potentially implicated in JF inflammation. CONCLUSION: MIF/CD74 axis elicited JF inflammation and may provide new therapeutic targets for joint capsule fibrosis in PTJC.

18.
FASEB J ; 35(3): e21405, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33559950

RESUMO

Microglia activation and release of pro-inflammatory cytokines have been closely linked to glaucoma. However, the mechanisms that initiate these pathways remain unclear. Here, we investigated the role of a pro-inflammatory cytokine--osteopontin (OPN), in retinal microglia activation process along with the underlying mechanisms in glaucoma. A rat chronic ocular hypertension (COH) model was established presenting an increase in retinal OPN level and activation of microglia. Primary microglia cells were isolated and cultured under a pressure culture system showing heightened expressions of microglia-derived OPN with changes in inflammatory factors (TNF-α, IL-1ß, and IL-6). OPN and OPN neutralizing antibody (Anti-OPN) interventions were both applied systems for comparison, and cross-referenced with OPN knockdown in vitro. JAK/STAT, NF-κB, ERK1/2, and p38 MAPK, recognized as the primary signaling pathways related to microglia activation, were then screened on whether they can facilitate OPN to act on microglia and their impact on specific inhibitors. Thereafter, retrograde labeling of retinal ganglion cells (RGCs) and flash visual evoked potentials (F-VEP) were used to investigate neuron protection in context of each blockade. Results suggest that OPN is able to enhance the proliferation and activation of retinal microglia in experimental glaucoma which may play a role in the glaucomatous optic neuropathy, and contribute to the eventual RGCs loss and vision function impairment. Such effect may be mediated through the regulation of p38 MAPK signaling pathway.

19.
Virol Sin ; 2021 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-33400092

RESUMO

To investigate the molecular epidemiology and genetic variation of human adenovirus type 7 (HAdV-7) in children with acute respiratory infections (ARI) in China. HAdV-7-positive respiratory samples collected from children with ARI in Beijing, Shijiazhuang, Wenzhou and Guangzhou from 2014-2018 were selected for gene amplification and sequence analysis. Fifty-seven HAdV-7 clinical strains with hexon, penton base and fiber gene sequences were obtained. Meanwhile 17 strains were selected randomly from different cities for whole genome sequencing. Phylogenetic and variation analyses were performed based on the obtained sequences, HAdV-7 prototype strain Gomen (AY594255), vaccine strains (AY495969 and AY594256) and representative sequences of strains. The phylogenetic trees constructed based on whole genome sequences, major capsid protein genes (hexon, penton base and fiber) and the early genes (E1, E2, E3 and E4) were not completely consistent. The HAdV-7 strains obtained in this study always clustered with most of the circulating strains worldwide from the 1980s to the present. Compared with the HAdV-7 prototype strain Gomen (AY594255), some amino acid mutations in loop1 and loop2 of hexon and the RGD loop region of the penton base gene were observed. Recombination analysis showed that partial regions of 55 kDa protein and 100 kDa hexon-assembly associated protein genes among all HAdV-7 strains in this study were from HAdV-16 and HAdV-3, respectively. Our study demonstrated the molecular evolution characteristics of HAdV-7 strains circulating in China and provided basic reference data for the prevention, control and vaccine development of HAdV-7.

20.
Birth Defects Res ; 2021 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-33470562

RESUMO

BACKGROUND: Mutations in genes associated with deafness differ between ethnic groups and regions in China. In this study, we investigated the genes associated with deafness in pregnant women to analyze the distribution of mutations leading to deafness in Zhuzhou, China. METHODS: A total of 10,684 pregnant women were enrolled in this study. DNA samples were collected to detect the 14 common mutations in deafness genes (at 108 sites). RESULTS: Prevalence of mutations in deafness genes in pregnant women with normal hearing in Zhuzhou was 4.92% (526/10,684). Among these 526 pregnant women with deafness gene mutations, the frequencies of mutated GJB2, GJB3, SLC26A4, and mtDNA 12S rRNA were 40.11, 7.22, 40.68, and 11.98%, respectively. The hotspots for mutations in the deafness genes were: c.235delC in GJB2 (31.18%), c.919-2A > G in SLC26A4 (18.44%), c.299_300delAT in GJB2 (5.70%), m.7444G > A in mtDNA 12S rRNA (5.70%), c.1229C > T in SLC26A4 (5.51%), m.1555A > G in mtDNA 12S rRNA (5.32%), accounting for 71.85%. Moreover, husbands of the 526 pregnant women who carried the deafness gene mutations were also included in the analysis to detect deafness gene mutations. Among the 526 husbands, 23 husbands carried mutations in deafness genes, accounting for 4.37%. The deafness gene mutations of the husbands and pregnant wives were not the same. In addition, the results of the neonatal follow-up hearing tests were all normal. CONCLUSION: Our study identified the prevalence of mutations in GJB2, SLC26A4, mtDNA 12S rRNA, and GJB3 genes in pregnant women from Zhuzhou, China.

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