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1.
Artigo em Inglês | MEDLINE | ID: mdl-33533180

RESUMO

Vibrios are widespread in both marine and coastal water environments and are recognized as one of the most important prokaryotic pathogens because they may potentially threaten the health of both aquacultures and human beings. However, owing to highly similar physiological and biochemical properties, accurate classification and identification of Vibrio strains remains challenging. This hampers further research on the physiology, pathogeny, genomics, epidemics, and ecology of vibrios. Here, we comparatively evaluated multiple approaches including 16S rRNA gene identity, average nucleotide identity (ANI), gene content similarity and mutilocus sequence analysis (MLSA) to investigate their ability in delineating Vibrio strains. In addition, we also evaluated the possibility of applying bacterial prophages in classifying and identifying Vibrio strains. Our results showed that MLSA outperformed other methods in discriminating Vibrio species, suggesting that the other four approaches should be used with cautions in Vibrio delineation. Interestingly, we also found that prophages identified in Vibrio strains were highly specific at strain- and species-level, suggesting that prophages held the potential to be used for microbial species, sub-species, and strain-level identifications. This study is expected to provide valuable insights into the taxonomic identification and classification of complex microbial groups in the post-genomic era.

2.
ACS Nano ; 2021 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-33491450

RESUMO

Two-dimensional covalent organic frameworks (2D-COFs) may serve as an emerging family of catalysts with well-defined atomic structures. However, the severe stacking of 2D nanosheets and large intrinsic bandgaps significantly impair their catalytic performance. Here, we report coaxial one-dimensional van der Waals heterostructures (1D vdWHs) comprised of a carbon nanotube (CNT) core and a thickness tunable thienothiophene-pyrene COF shell using a solution-based in situ wrapping method. Density functional theory calculations and operando and ex situ spectroscopic analysis indicate that carbon-sulfur regions in thienothiophene groups in the COF serve as an active catalytic site for oxygen reduction and evolution reactions. The coaxial structure enables n-doping from the CNT core to the COF shell, which is controllable by varying COF shell thickness. The charge transfer from CNTs lowers COF's bandgap and work function, which reduces the charge transfer barrier between the active catalytic sites and adsorbed oxygen intermediates, resulting in dramatically enhanced catalytic activity. The 1D vdWHs were applied as a bifunctional oxygen electrocatalyst in rechargeable zinc-air batteries, delivering a high specific capacity of 696 mAh gZn-1 under a high current density of 40 mA cm-2 and excellent cycling stability. The 1D vdWHs based on the coaxial structure of 2D COF wrapped around CNT cores may be further used as versatile building units to create multidimensional vdWHs for exploring fundamental physics and chemistry as well as practical applications in electrochemistry, electronics, photonics, and beyond.

3.
Analyst ; 2021 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-33496685

RESUMO

Among antibacterial nanomaterials, carbon dots (CDs) have attracted much attention because of their unique physical and chemical properties and good biosafety. In this study, kanamycin sulfate (Kan), a broad-spectrum antibiotic, was used to synthesize novel carbon dots (CDs-Kan) by a one-step hydrothermal method. CDs-Kan showed good inhibitory effects on Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus. Further, scanning electron microscopy revealed that treatment with CDs-Kan and Kan resulted in the same phenomena. In particular, the morphologies of S. aureus cells treated with CDs-Kan and Kan became smaller and irregular, whereas the surfaces of E. coli cells protruded and formed vesicles. These results indicated that CDs-Kan was shown to retain the good antibacterial activity of Kan as well as its main bactericidal functional groups, namely, the amino sugar and amino cyclic alcohol, We refer to this phenomenon as the "preservation property". We also found that CDs-Kan has good biocompatibility and nontoxic properties. Moreover, CDs-Kan was successfully applied to the biological imaging of fungi and plant cells. In addition, CDs-Kan could be used as a fluorescent probe for the quick, sensitive, and selective detection of Cr6+. Therefore, CDs-Kan not only retained the good bacteriostatic properties of Kan but also expanded its application in bioimaging and biosensors.

4.
ISME J ; 2021 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-33479492

RESUMO

Profound biogeochemical responses of anoxic sediments to the fluctuation of dissolved oxygen (DO) concentration in overlaying water are often observed, despite oxygen having a limited permeability in sediments. This contradiction is indicative of previously unrecognized mechanism that bridges the oxic and anoxic sediment layers. Using sediments from an urban river suffering from long-term polycyclic aromatic hydrocarbons (PAHs) contamination, we analyzed the physicochemical and microbial responses to artificially elevated DO (eDO) in the overlying water over 9 weeks of incubation. Significant changes in key environmental parameters and microbial diversity were detected over the 0-6 cm sediment depth, along with accelerated degradation of PAHs, despite that eDO only increased the porewater DO in the millimeter subfacial layer. The dynamics of physicochemical and microbial properties coincided well with significantly increased presence of centimeter-long sulfide-oxidizing cable bacteria filaments under eDO, and were predominantly driven by cable bacteria metabolic activities. Phylogenetic ecological network analyses further revealed that eDO reinforced cable bacteria associated interspecific interactions with functional microorganisms such as sulfate reducers, PAHs degraders, and electroactive microbes, suggesting enhanced microbial syntrophy taking advantage of cable bacteria metabolism for the regeneration of SO42- and long-distance electron transfer. Together, our results suggest cable bacteria may mediate the impacts of eDO in anaerobic sediments by altering sediment physiochemical properties and by reinforcing community interactions. Our findings highlight the ecological importance of cable bacteria in sediments.

5.
Sci Total Environ ; 750: 141513, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-32853935

RESUMO

Excessive nitrate in water systems is prevailing and a global risk of human health. Polluted river sediments are dominated by anaerobes and often the hotspot of denitrification. So far, little is known about the ecological effects of nitrate pollution on microbial dynamics, especially those in sulfide-rich sediments. Here we simulated a nitrate surge and monitored the microbial responses, as well as the changes of important environmental parameters in a sulfide-rich river sediment for a month. Our analysis of sediment microbial communities showed that elevated nitrate led to (i) a functional convergence at denitrification and sulfide oxidation, (ii) a taxonomic convergence at Proteobacteria, and (iii) a significant loss of biodiversity, community stability and other functions. Two chemolithotrophic denitrifiers Thiobacillus and Luteimonas were enriched after nitrate amendment, although the original communities were dominated by methanogens and syntrophic bacteria. Also, serial dilutions of sediment microbial communities found that Thiobacillus thiophilus dominated 18/30 communities because of its capability of simultaneous nitrate reduction and sulfide oxidation. Additionally, our network analysis indicated that keystone taxa seemed more likely to be native auxotrophs (e.g., syntrophic bacteria, methanogens) rather than dominant denitrifiers, possibly because of the extensive interspecific cross-feeding they estabilished, while environment perturbations probably disrupted that cross-feeding and simplified microbial interactions. This study advances our understanding of microbial community responses to nitrate pollution and possible mechanism in the sulfide-rich river sediment.


Assuntos
Microbiota , Rios , Desnitrificação , Sedimentos Geológicos , Nitratos , Sulfetos , Thiobacillus
6.
Environ Microbiol ; 2020 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-33295091

RESUMO

Thermoplasmata is a widely-distributed and ecologically-important archaeal class in the phylum Euryarchaeota. Because few cultures and genomes are available, uncharacterized Thermoplasmata metabolisms remain unexplored. In this study, we obtained four medium- to high-quality archaeal metagenome-assembled genomes (MAGs) from the filamentous fragments of black-odorous aquatic sediments (Foshan, Guangdong, China). Based on their 16S rRNA gene and ribosomal protein phylogenies, the four MAGs belong to the previously unnamed Thermoplasmata UBA10834 clade. We propose that this clade (five reference genomes from the Genome Taxonomy Database (GTDB) and four MAGs from this study) be considered a new order, Candidatus Gimiplasmatales. Metabolic pathway reconstructions indicated that the Ca. Gimiplasmatales MAGs can biosynthesize isoprenoids and nucleotides de novo. Additionally, some taxa have genes for formaldehyde and acetate assimilation, and the Wood-Ljungdahl CO2 -fixation pathway, indicating a mixotrophic lifestyle. Sulfur reduction, hydrogen metabolism, and arsenic detoxification pathways were predicted, indicating sulfur-, hydrogen-, and arsenic-transformation potentials. Comparative genomics indicated that the H4 F Wood-Ljungdahl pathway of both Ca. Gimiplasmatales and Methanomassiliicoccales was likely obtained by the interdomain lateral gene transfer from the Firmicutes. Collectively, this study elucidates the taxonomic and potential metabolic diversity of the new order Ca. Gimiplasmatales and the evolution of this subgroup and its sister lineage Methanomassiliicoccales. This article is protected by copyright. All rights reserved.

7.
J Hazard Mater ; : 124385, 2020 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-33229269

RESUMO

Microbial sulfate-reduction coupling polycyclic aromatic hydrocarbon (PAH) degradation is an important process for the remediation of contaminated sediments. However, little is known about core players and their mechanisms in this process due to the complexity of PAH degradation and the large number of microorganisms involved. Here we analyzed potential core players in a black-odorous sediment using gradient-dilution culturing, isolation and genomic/metagenomic approaches. Along the dilution gradient, microbial PAH degradation and sulfate consumption were not decreased, and even a significant (p = 0.003) increase was observed in the degradation of phenanthrene although the microbial diversity declined. Two species, affiliated with Desulfovibrio and Petrimonas, were commonly present in all of the gradients as keystone taxa and showed as the dominant microorganisms in the single colony (SB8) isolated from the highest dilution culture with 93.49% and 4.73% of the microbial community, respectively. Desulfovibrio sp. SB8 and Petrimonas sp. SB8 could serve together as core players for sulfate-reduction coupling PAH degradation, in which Desulfovibrio sp. SB8 could degrade PAHs to hexahydro-2-naphthoyl through the carboxylation pathway while Petrimonas sp. SB8 might degrade intermediate metabolites of PAHs. This study provides new insights into the microbial sulfate-reduction coupling PAH degradation in black-odorous sediments.

8.
Artigo em Inglês | MEDLINE | ID: mdl-33158896

RESUMO

Iron-reducing microorganisms (FeRM) play key roles in many natural and engineering processes. Visualizing and isolating FeRM from multispecies samples are essential to understand the in-situ location and geochemical role of FeRM. Here, we visualized FeRM by a "turn-on" Fe2+-specific fluorescent chemodosimeter (FSFC) with high sensitivity, selectivity and stability. This FSFC could selectively identify and locate active FeRM from either pure culture, co-culture of different bacteria or sediment-containing samples. Fluorescent intensity of the FSFC could be used as an indicator of Fe2+ concentration in bacterial cultures. By integrating FSFC with a single cell sorter, we obtained three FSFC-labeled cells from an enriched consortia and all of them were subsequently evidenced to be capable of iron-reduction and two unlabeled cells were evidenced to have no iron-reducing capability, further confirming the feasibility of the FSFC.IMPORTANCE Visualization and isolation of FeRM from samples containing multispecies are commonly needed by researchers from different disciplines, such as environmental microbiology, environmental sciences and geochemistry. However, no available method has been reported. In this study, we provide a solution to visualize FeRM and evaluate their activity even at single cell level. Integrating with single cell sorter, FeRM can also be isolated from samples containing multispecies. This method can be used as a powerful tool to uncover the in-situ or ex-situ role of FeRM and their interactions with ambient microbes or chemicals.

9.
AMB Express ; 10(1): 199, 2020 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-33140118

RESUMO

Bacteria promoters along with operators are crucial elements in the control of gene expression in microbes in response to environmental stress changes. A genome-wide promoter DNA regulatory library is in demand to be developed for a microbe reporter method to monitor the existence of any given environmental stress substance. In this study, we utilized Escherichia coli (E. coli) as a model system for the preparation of both cell lysates and genomic DNA fragments. Through enriching protein-bound DNA fragments to construct luciferase reporter libraries, we found that, of 280 clones collected and sequenced, 131 clones contained either the promoter-35 and -10 conservative sequences and/or an operator transcription factor binding sites (TFBS) region. To demonstrate the functionality of the identified clones, five of 131 clones containing LexA binding sequence have been demonstrated to be induced in response to mitomycin C treatment. To evaluate our libraries as a functional screening library, 80 randomly picked up clones were cultured and treated with and without MMC, where two clones were shown to have greater than twofold induction. In addition, two arsenite-responsive clones were identified from 90 clones, one having the well-known ArsR and another having the osmotically inducible lipoprotein (OsmE1). The newly discovered osmE1 has been quantitatively validated to be induced by arsenite treatment with real-time PCR in a dose response and time course manner. This enriching protein-bound DNA luciferase reporter libraries and functional screening facilitate the identification of stress-responsive transcriptional factors in microbes. We developed functional libraries containing E. coli genomic-wide protein-bound DNA as enhancers/operators to regulate downstream luciferase in response to stress.

11.
Environ Int ; 144: 106068, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32871382

RESUMO

Atmospheric CO2 concentration is increasing, largely due to anthropogenic activities. Previous studies of individual free-air CO2 enrichment (FACE) experimental sites have shown significant impacts of elevated CO2 (eCO2) on soil microbial communities; however, no common microbial response patterns have yet emerged, challenging our ability to predict ecosystem functioning and sustainability in the future eCO2 environment. Here we analyzed 66 soil microbial communities from five FACE sites, and showed common microbial response patterns to eCO2, especially for key functional genes involved in carbon and nitrogen fixation (e.g., pcc/acc for carbon fixation, nifH for nitrogen fixation), carbon decomposition (e.g., amyA and pulA for labile carbon decomposition, mnp and lcc for recalcitrant carbon decomposition), and greenhouse gas emissions (e.g., mcrA for methane production, norB for nitrous oxide production) across five FACE sites. Also, the relative abundance of those key genes was generally increased and directionally associated with increased biomass, soil carbon decomposition, and soil moisture. In addition, a further literature survey of more disparate FACE experimental sites indicated increased biomass, soil carbon decay, nitrogen fixation, methane and nitrous oxide emissions, plant and soil carbon and nitrogen under eCO2. A conceptual framework was developed to link commonly responsive functional genes with ecosystem processes, such as pcc/acc vs. soil carbon storage, amyA/pulA/mnp/lcc vs. soil carbon decomposition, and nifH vs. nitrogen availability, suggesting that such common responses of microbial functional genes may have the potential to predict ecosystem functioning and sustainability in the future eCO2 environment.

12.
Antonie Van Leeuwenhoek ; 113(11): 1713-1714, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32856220

RESUMO

In the published version of the article, the title should have read 'Duganella rivi sp. nov., Duganella fentianensis sp. nov., Duganella qianjiadongensis sp. nov. and Massilia guangdongensis sp. nov., isolated from subtropical streams in China and reclassification of all species within genus Pseudoduganella'.

13.
Sci Total Environ ; 748: 141328, 2020 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-32798868

RESUMO

Iron-redox cycling microorganisms are important for understanding the biogeochemical iron and play key roles in zero-valent iron (ZVI) mediated environmental bioremediation. Their influence on ZVI oxidation coupling with organic contaminant removal is of particular interest but is still poorly understood. The objective of this research was to study microbial redox cycles of iron in ZVI oxidation and deca-brominated diphenyl ether (deca-BDE) removal. It was found that iron-oxidizing bacteria (IOB) enhanced ZVI oxidation by using iron as the sole electron donor. Iron-reducing bacteria (IRB) with high activity of Fe (III) reduction, also significantly accelerated rather than inhibited ZVI oxidation. ZVI oxidation activity was increased from 3.42% to 24.28% by IOB and 19.49% by IRB. When deca-BDE was present in the medium, ZVI oxidation activity by IOB and IRB was increased from 2.67% to 48.33% and 64.33%, respectively. However, no co-accelerating effect of IOB and IRB occurred but rather a neutralizing influence on ZVI oxidation was detected with iron-redox cycling bacteria (IORB). ZVI oxidation activity by IORB only increased to 13.14% and 37.0% in the absence and presence of deca-BDE, respectively. Meanwhile, IRB also exhibited the highest removal activity of deca-BDE. Approximately 71.67% of deca-BDE was removed by IRB, compared to 18.91% by IOB and 43.24% by IORB. Deca-BDE significantly influenced the effects of iron-metabolizing microorganisms on ZVI oxidation by altering the composition of microbial communities. Pseudomonas, Paenibacillus, and Sporolactobacillus were the key genera influencing ZVI oxidation and deca-BDE removal. Sporolactobacillus was firstly reported to be able to stimulate both ZVI oxidation and deca-BDE removal. Pseudomonas accelerated ZVI oxidation but had no significant contribution to deca-BDE removal. However, Paenibacillus inhibited both Fe(III) reduction and deca-BDE removal. It is expected that continuous integration of ZVI oxidation and organic contaminant removal can be achieved by regulating the key genera in iron-metabolizing microbial communities.


Assuntos
Bifenil Polibromatos , Poluentes Químicos da Água , Éter , Éteres Difenil Halogenados , Ferro , Oxirredução
14.
Int J Syst Evol Microbiol ; 70(10): 5205-5210, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32816658

RESUMO

A Gram stain-positive, non-spore-forming, non-motile and rod-shaped actinomycete, strain 5221T, was isolated from the sediment of a river collected at Ronggui in the Pearl River Delta, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain formed a distinct lineage within the genus Brevibacterium and had the highest sequence similarity to Brevibacterium pityocampae Tp12T (96.7 %), followed by Brevibacterium daeguense 2C6-41T (96.5 %), Brevibacterium samyangense SST-8T (96.0 %) and Brevibacterium ravenspurgense 20T (95.9 %). The results of chemotaxonomic analyses, including detecting anteiso-C15 : 0, anteiso-C17 : 0, and C16 : 0 as the major cellular fatty acids, diphosphatidylglycerol, phosphatidylglycerol and three phosphoglycolipids as the polar lipids, MK-8(H2) as the major menaquinone, and a DNA G+C content of 72.4 mol%, supported that strain 5221T is a member of the genus Brevibacterium. Furthermore, low sequence similarities of 16S rRNA gene sequences, differences in fatty acid compositions and differential physiological characteristics such as enzyme activity and carbon sources utilization ability distinguished the isolate from its close relatives. Therefore, strain 5221T represents a novel species of the genus Brevibacterium, for which the name Brevibacterium rongguiense sp. nov. is proposed, with the type strain 5221T (=GDMCC 1.1766T=KACC 21700T).


Assuntos
Brevibacterium/classificação , Sedimentos Geológicos/microbiologia , Filogenia , Rios/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , Brevibacterium/isolamento & purificação , China , DNA Bacteriano/genética , Ácidos Graxos/química , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
15.
Curr Microbiol ; 77(10): 3185-3191, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32601835

RESUMO

A Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and motile strain FT127WT was isolated from a subtropical stream in China. Comparison based on 16S rRNA gene sequences showed that strain FT127WT belongs to genus Massilia and shares 98.5% similarity with Massilia buxea A9T as its closest neighbor. The genome size of strain FT127WT was 6.65 Mbp with G + C content of 65.3%. The calculated pairwise OrthoANIu values and digital DNA-DNA hybridization values between strain FT127WT and each of strains M. buxea KCTC 52429T, Massilia armeniaca ZMN-3T, Massilia plicata DSM 17505T and Massilia namucuonensis CGMCC 1.11014T were less than 83.1% and 26.6%, respectively. The reconstructed phylogenomic tree based on concatenated 92 core genes showed that strain FT127WT clusters closely with M. namucuonensis CGMCC 1.11014T. The respiratory quinone of strain FT127WT was Q-8. The major fatty acids were C16:1 ω7c, C16:0 and C12:0. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid. Combining above all characteristics, strain FT127WT should represent a novel species within genus Massilia, for which the name Massilia aquatica sp. nov. (type strain FT127WT = GDMCC 1.1690T = KACC 21482T) is proposed.

16.
Int J Syst Evol Microbiol ; 70(8): 4822-4830, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32706332

RESUMO

Five Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and motile strains (FT50WT, FT80WT, FT92WT, FT94W and FT135WT) were isolated from a subtropical stream in PR China. Comparisons based on 16S rRNA gene sequences showed that strains FT50WT, FT94W and FT135WT take strain Duganella sacchari Sac-22T, and strains FT80WT and FT92WT take strain Duganella ginsengisoli DCY83T as their closest neighbour in the phylogenetic trees, respectively. The G+C contents of strains FT50WT, FT80WT, FT92WT, FT94W and FT135WT were 63.3, 62.4, 62.8, 63.8 and 60.8 %, respectively. The reconstructed phylogenomic tree based on concatenated 92 core genes showed that strains FT50WT, FT80WT, FT94W and FT135WT clustered together with species of the genus Duganella, but strains FT92WT and D. ginsengisoli KCTC 42409T were located in the clades of the genus Massilia. The calculated pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values among strains FT50WT, FT80WT, FT92WT, FT94W, FT135WT and related strains were in the ranges of 75.6-87.8% and 20.3-33.8% except that the values between strains FT50WT and FT94W were 98.7 and 89.2%, respectively. The respiratory quinone of these five strains was Q-8. The major fatty acids were C16 : 1 ω7c, C16 : 0, C18 : 1 ω7c and C12 : 0. The polar lipids included phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid. Considering the distinct phylogenetic relationships of D. ginsengisoli with species of the genus Massilia in the phylogenomic tree, it was reasonable to transfer D. ginsengisoli to the genus Massilia as Massilia ginsengisoli comb. nov. Combining the results of phylogenomic analysis, ANI and dDDH data, and a range of physiological and biochemical characteristics together, strains FT50WT and FT94W should belong to the same species and be assigned to genus Duganella with strains FT80WT and FT135WT together, and strain FT92WT should be assigned to the genus Massilia, for which the names Duganella lactea sp. nov. (type strain FT50WT=GDMCC 1.1674T=KACC 21466T), Duganella guangzhouensis sp. nov. (FT80WT=GDMCC 1.1678T=KACC 21470T), Duganella flavida sp. nov. (FT135WT=GDMCC 1.1745T=KACC 21659T) and Massilia rivuli sp. nov. (FT92WT=GDMCC 1.1682T=KACC 21474T) are proposed.


Assuntos
Oxalobacteraceae/classificação , Filogenia , Rios/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Oxalobacteraceae/isolamento & purificação , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
18.
J Cardiothorac Surg ; 15(1): 189, 2020 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-32711546

RESUMO

BACKGROUND: The objective of this study was to explore the consistency and correlation of two troponin (cTn) subtypes, troponin I (cTnI) and high-sensitivity troponin T (hs-cTnT), which can be used to judge early myocardial injury after curative resection of oesophageal cancer. METHODS: This study is a secondary analysis of data obtained from a previous randomized controlled trial on postoperative myocardial injury in 70 patients undergoing elective curative resection of oesophageal cancer who were randomly assigned to undergo aggressive body temperature management (nasopharyngeal temperature 36.61 ± 0.18 °C) or standard body temperature management (35.80 ± 0.18 °C, n = 35 in each arm). The serum cTnI and hs-cTnT levels were measured in each patient at the 4 time points: before the operation and 6 h ~ 12 h, 24 h and 48 h after the operation. The diagnostic criteria of myocardial injury followed the third edition ESC/ACCF definition of myocardial infarction. The primary outcomes included the following: (1) the incidence of myocardial injury and the relationship between hs-cTnT and cTn and (2) the consistency and correlation of the two cTn subtypes. RESULTS: A total of 280 pairs of cTn samples were tested. The incidence of postoperative day 2 myocardial injury was 8.6% (3/35) among patients receiving aggressive body temperature management and 31.4% (11/35) among patients receiving standard body temperature management (P < 0.05). Among 3 patients who experienced myocardial injury in the aggressive body temperature management group, 2 met the diagnostic criteria for cTnI and hs-cTnT and only 1 met the diagnostic criteria for hs-cTnT. Among the 11 patients who experienced myocardial injury in the standard body temperature management group, 7 met the diagnostic criteria for cTnI and hs-cTnT and only 3 met the diagnostic criteria for hs-cTnT; only 1 met the diagnostic criteria for cTnI. The bias of cTnI and hs-cTnT was - 8.82 ± 31.91 ng/L. The consistency limit was - 71.37 ~ 53.73 ng/L. The proportion within the scope of the consistency of its corresponding boundary was 98.57%. The correlation coefficient of cTnI and hs-cTnT was 0.845 (P < 0.05). CONCLUSIONS: In the evaluation of postoperative myocardial injury in patients undergoing curative resection of oesophageal cancer, cTnI and hs-cTnT exhibit high consistency and a good correlation. The combination of cTnI and hs-cTnT can improve the detection rate of myocardial injury, thus providing a better reference than a single measure alone for reducing the risk of perioperative myocardial injury in patients. TRIAL REGISTRATION: ChiCTR-INR-17011621 . Registered June 10, 2017.

19.
Chem Commun (Camb) ; 56(59): 8301-8304, 2020 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-32573628

RESUMO

A viscosity-sensitive fluorescent probe is demonstrated by grafting a rotatable 4-N,N'-dimethylaniline group on (6,5) single-walled carbon nanotubes. The rotation of the grafted group is constricted by solution viscosity, causing changes in the photoluminescence behaviors of the nanotubes. This enables a highly sensitive fluorescent probe for determining solution ratiometric viscosity in the biologically transparent second near-infrared region.

20.
J Cardiothorac Vasc Anesth ; 34(9): 2413-2418, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32381306

RESUMO

OBJECTIVES: The objective of this study was to explore the prevalence of undiagnosed mild cognitive impairment (MCI) and its association with adverse outcomes in elderly patients undergoing thoracic surgery. DESIGN: A prospective cohort study. SETTING: Large tertiary medical center. PARTICIPANTS: The authors enrolled 170 patients aged 65 years or older who were scheduled for thoracic surgery between November 7, 2018, and April 1, 2019, at the Shanghai Chest Hospital. Patients with a history of schizophrenia or dementia disease, uncorrected vision or hearing impairment, and refusal to participate were excluded. INTERVENTIONS: A total of 154 elderly patients completed the Chinese version of the Montreal Cognitive Assessment (MoCA) test preoperatively and were included in the final analysis. They were categorized into a normal group (MoCA ≥ 26 scores, group N) and an abnormal group (MoCA < 26 scores, group AN) based on test results. Delirium was assessed with the Confusion Assessment Method twice daily during the first 3 postoperative days. MEASUREMENTS AND MAIN RESULTS: The primary outcome was the incidence of postoperative delirium (POD). Secondary outcomes included the incidence of postoperative pulmonary complications (PPCs), cardiovascular complications, other complications, intensive care unit (ICU) stay, and the hospital length of stay (LOS). The incidence of MCI before thoracic surgery in elderly patients was 49.4% (76 of 154). Compared with group N, MCI could increase the incidence of POD (14.1% v 30.3%, p = 0.016) and median LOS (4 d v 5 d, p = 0.016). However, the differences in pulmonary complications, cardiovascular and other complications, and ICU stay were not significant. Multivariable logistic regression analysis showed preoperative MCI (OR = 2.573, 95% CI =1.092 to 6.060, p = 0.031) as an independent risk factor of POD. Compared with the elderly patients without POD, POD could increase the risk of PPCs (17.5% v 35.3%, p = 0.026) and median LOS (4 d v 5 d, p < 0.001). CONCLUSIONS: The incidence of MCI before thoracic surgery in elderly patients was higher and associated with a higher rate of adverse postoperative outcomes. The findings may be important for preoperative patient counseling, operative planning, and eventually reducing potential risk exposure and related outcomes.

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