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1.
Cancer Med ; 2019 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-31502764

RESUMO

To investigate the incidence and risk factors as well as prognosis of autoimmune hemolytic anemia (AIHA) following allogeneic hematopoietic stem cell transplantation (allo-HSCT), a total of 1377 adult hematological malignancies at three institutions were enrolled in this study. The 3-year cumulative incidence of AIHA was 2.2 ± 0.4%. Multivariate analysis showed that haploidentical donors (HRDs) and chronic graft vs host disease (cGVHD) were the independent risk factors for AIHA. Patients with AIHA treated initially with corticosteroids combined with cyclosporine A (CsA) had a higher complete response rate than those with corticosteroids monotherapy (66.7% vs 11.1%; P = .013). The 3-year cumulative incidence of malignant diseases relapse was 4.4 ± 4.3% and 28.0 ± 1.3% (P = .013), treatment-related mortality (TRM) was 8.9 ± 6.3% and 17.4 ± 1.2% (P = .431), disease-free survival (DFS) was 56.1 ± 1.5% and 86.7 ± 7.2% (P = .011), and overall survival (OS) was 86.3 ± 7.4% and 64.1 ± 1.5% (P = .054), respectively, in the patients with AIHA and those without AIHA. Our results indicate that HRDs and cGVHD are risk factors for AIHA and corticosteroids combined with CsA are superior to corticosteroids as initial treatment for AIHA. Autoimmune hemolytic anemia does not contribute to increase TRM and could reduce the malignant diseases relapse and increase DFS.

2.
Redox Rep ; 24(1): 62-69, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31438780

RESUMO

Objective: Resveratrol has been confirmed to improve bone quality and delay osteoporosis, but the mechanisms have not been thoroughly elucidated. In this report, we investigated the osteogenic differentiation effect of resveratrol on senescent bone mesenchymal stem cells (BMSCs) and the involvement of AMP-activated protein kinase (AMPK)/ reactive oxygen species (ROS) signaling pathway. Methods: Cell senescence, viability, and osteogenic differentiation of BMSCs influenced by resveratrol were investigated and ROS production and AMPK expression were detected. Results: Cell senescence, characterized by senescence ß-galactosidase staining and senescence-related genes (p16, p21, and p53) expression, was attenuated by resveratrol. Cell viability, extracellular matrix calcification, and osteogenic-related genes expression were significantly enhanced after resveratrol treatment. ROS production in BMSCs was inhibited while AMPK expression was up-regulated by resveratrol. Inhibition of AMPK expression by compound C reduced resveratrol-prompted osteogenesis and ROS production down-regulation. Conclusion: These results provide a potential mechanism involving AMPK activation/ROS inhibition signaling pathway in osteogenic differentiation of BMSCs enhanced by resveratrol. It suggests that development of therapy towards ROS is an effective way for osteoporosis treatment.

3.
Am J Nephrol ; : 1-15, 2019 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-31454822

RESUMO

BACKGROUND: Inadequate dialysis, renal hypertension, and impaired exercise capacity are factors that affect the quality of life (QoL) and mortality of adults with end-stage renal disease (ESRD) undergoing hemodialysis (HD). This systematic review provided valid evidence about the effect of exercise training on single-pool Kt/V (sp Kt/V), blood pressure, and peak uptake oxygen (VO2 peak). METHOD: A systematic review and meta-analysis of published randomized controlled trials (RCTs) that evaluated the effects of no <8 weeks' exercise training on the physical fitness outcomes for adults with ESRD undergoing HD were accepted in this study. RESULTS: Included 20 trials (677 participants) indicated that various exercise types improved aerobic capacity, walking capacity, and health-related QoL totally. Of note, aerobic exercise and combined exercise were the predominant exercise types. CONCLUSION: Based on our evidence, aerobic exercise or combined exercise at least for 8 weeks to 12 months, 3 times weekly, will be beneficial to physical conditions of the patients with ESRD undergoing HD. The clinical staff can treat patients with the evidence above. Future studies need to provide more information basis for the construction of patient exercise system by adding various exercise combinations.

4.
Chem Commun (Camb) ; 55(72): 10701-10704, 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31429464

RESUMO

Newfangled frangipani-like [MV5O6(µ3-O)5(SO4)(COO)5] (M = Nb/W) polyanions served as 5-connected molecular building blocks (MBBs) that simultaneously assembled with 4-connected [V5O9Cl] MBBs and tricarboxylate ligands (H3BTC) to form two new polyoxovanadate-based metal-organic polyhedra {[MV5O6(µ3-O)5(SO4)]4[V5O9Cl]4(BTC)12} with undiscovered "near-miss Johnson solid" geometry. Moreover, the variable-temperature magnetic susceptibilities were investigated.

5.
Oral Dis ; 2019 Aug 08.
Artigo em Inglês | MEDLINE | ID: mdl-31393636

RESUMO

OBJECTIVE: There are challenges in the treatment of chronic inflammatory diseases of oral mucosa. Both paeoniflorin (PF) and baicalin (BAI) exert anti-inflammatory effects, but the mechanism underlying their combined effects is still unclear. Here, we explored the anti-inflammatory function of the PF-BAI combination in the oral inflammatory response. MATERIALS AND METHODS: The CCK-8 assay was used to determine the proliferative capacity of HOKs with PF and BAI. Enzyme-linked immunosorbent (ELISA), Western blotting, reverse transcription polymerase chain reaction, and confocal immunofluorescence were performed to study the anti-inflammatory effects of PF-BAI in LPS-stimulated human oral keratinocytes (HOKs). Immunohistochemistry and ELISA were performed to detect the levels of NF-κB p65, IKKα and IL-6, TNF-α in OLP and healthy tissues. RESULTS: Compared to PF or BAI alone, the combination of PF-BAI at 5 µg/ml downregulated secretion of inflammatory cytokines more effectively (p < .05). Combined PF-BAI decreased NF-κB p65 and IκBα protein phosphorylation, leading to reduce nuclear translocation of NF-κB p65. Higher expression of TNF-α, IL-6, NF-κB p65, and IKKα were observed in OLP than in HC tissues (p < .01). CONCLUSION: The optimal combination concentration of PF and BAI at 5 µg/ml may have a positive effect on the treatment of oral inflammatory diseases, providing a novel therapeutic approach.

6.
J Cardiol ; 2019 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-31416781

RESUMO

BACKGROUND: Inflammation plays a pivotal role in coronary artery disease (CAD). Few data from large-size studies are available on the association of high-sensitivity C-reactive protein (hs-CRP) and severity of CAD. Our aim was to investigate their relationship as well as their impact on long-term outcomes in patients undergoing percutaneous coronary intervention. METHODS: In 2013, 10,020 patients were consecutively included. Patients were divided into three groups based on hs-CRP on admission: 0-3mg/L (n=6978, 69.6%), 3.01-10mg/L (n=1997, 19.9%), >10mg/L (n=1045, 10.4%). Disease severity was determined by SYNTAX score (SS). Their differences were assessed in SS and major adverse cardiovascular events (MACEs, including all-cause death, myocardial infarction, revascularization, and in-stent thrombosis) among groups. RESULTS: The mean follow-up period was 874 days. Patients with elevated hs-CRP were older, had more risk factors such as hypertension, cerebrovascular disease, chronic obstructive pulmonary disease, and cigarette smoking. Multivariate regression analysis showed that hs-CRP >10mg/L (OR 1.49, 95% confidence interval 1.21-1.84, p<0.001), age, previous myocardial infarction, serum creatinine, and left ventricular ejection fraction were independent predictors of intermediate-high SS (>22). Subgroup analysis indicated that the relation between hs-CRP and SS was also consistent in acute coronary syndrome and its subtypes. Although elevated hs-CRP was positively associated with increased rates of MACEs (11.0% versus 12.1% versus 14.3%, p=0.006), death (1.0% versus 1.3% versus 3.0%, p<0.001), and revascularization (8.6% versus 10.4% versus 10.0%, p=0.032), it did not show any prognostic effect for adverse outcomes in multivariate regression analyses (all adjusted p> 0.05). While SS>22 remained independently predictive of MACEs and revascularization after adjusting confounders, the risks of which were increased by 56% and 68%, respectively. CONCLUSION: Serum hs-CRP could be a useful biomarker for indicating CAD severity and could aid in risk stratification.

7.
BMC Med ; 17(1): 156, 2019 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-31401973

RESUMO

BACKGROUND: The optimal dose of rabbit antithymocyte globulin (ATG, ImtixSangstat) minimizing infections without increasing graft-versus-host disease (GVHD) is unknown in T cell-replete, G-CSF-primed haploidentical hematopoietic stem cell transplantation (haplo-HSCT). METHODS: Four hundred and eight patients were enrolled in this multicenter study to evaluate the effect of 7.5 mg/kg and 10.0 mg/kg rabbit ATG on viral infections and GVHD prophylaxis after haplo-HSCT. The primary endpoint was EBV DNAemia within 1 year posttransplantation. RESULTS: The 1-year incidence of EBV DNAemia was 20.7% (95% confidence interval, 15.4-26.5) and 40.0% (33.3-46.6) in the 7.5 mg/kg and 10.0 mg/kg groups, respectively (P < 0.001). The 100-day cumulative incidence of grade II to IV aGVHD was 27.1% (21.1-33.4) and 25.4% (19.6-31.5) in the 7.5 mg/kg and 10.0 mg/kg ATG groups, respectively (P = 0.548). The 2-year incidence of chronic GVHD was 34.6% (27.8-41.4) and 36.2% (29.1-43.2) in the 7.5 mg and 10.0 mg groups (P = 0.814). The 1-year incidence of CMV DNAemia was 73.4% (67.2-79.4) and 83.4% (77.5-87.9) in the 7.5 mg/kg and 10.0 mg/kg groups (P = 0.038). The 3-year overall survival posttransplantation was 69.5% (63.2-75.8) and 63.5% (56.2-70.8), and the disease-free survival was 62.2% (55.3-69.1) and 60.3% (53.0-67.6) in the 7.5 mg/kg and 10.0 mg/kg groups, respectively (OS: P = 0.308; DFS: P = 0.660). The counts of EBV- and CMV-specific cytotoxic T cells (CTLs) were higher in the 7.5 mg/kg group than in the 10.0 mg/kg group early posttransplantation. CONCLUSIONS: Compared with 10.0 mg/kg, 7.5 mg/kg ATG for GVHD prophylaxis was associated with reduced EBV and CMV infections without increased incidence of GVHD in haplo-HSCT, probably by affecting EBV- and CMV-specific CTLs. TRIAL REGISTRATION: clinicaltrials.gov, NCT01883180 . Registered 14 June 2013.

8.
Disabil Health J ; : 100830, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31399346

RESUMO

BACKGROUND: For students with disabilities, evidence exists of significant links between body image evaluation and mental health status, and between coping strategies and mental health status, yet few investigations have tested body image evaluation, coping strategies and mental health status in one study to reveal their complicated relationships. OBJECTIVE: The present study investigated the mediation role of coping strategy between body image evaluation and mental health and its variations among Chinese university students with three types of disabilities, physical disability, visual disability and hearing disability. METHOD: Two hundred and fifty-five Chinese college students (166 males and 89 females) with disabilities completed a battery of self-report questionnaires, including Multidimensional Body-Self Relations Questionnaire (MBSRQ), Coping Style Questionnaire (CQS) and Symptom Checklist 90 (SCL-90). Mediation analyses were conducted in AMOS. RESULTS: In students with physical disabilities, positive coping strategies played a role in mediating the relation between body image evaluation and mental health (ß = -0.190, p < 0.05). In students with hearing disabilities, negative coping strategies played the mediation role (ß = -0.089, p < 0.05). No significant mediation effect was found in students with visual disabilities. Meanwhile, the mental health status of all of the three groups were significantly lower than the Chinese norm (M = 129.96; SD = 38.76) (all ps < 0.01). CONCLUSIONS: In Chinese college students with disabilities, in bridging the link between their body image evaluation and mental health status, coping strategies played a central role and its specific role varied depending upon the type of disability. Practical implications are discussed.

9.
Biomaterials ; 219: 119367, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31344514

RESUMO

Renal dysfunctions usually happen in viral infections and many viruses specially infect distal renal tubules, however the pathogenesis remains unknown. Here, in order to explore the pathogenesis of virus-related renal dysfunctions, a Pseudorabies Virus (PrV) induced kidney disease model was built on a distal tubule-on-a-chip (DTC), for the first time. The barrier structure and Na reabsorption of distal renal tubules were successfully reconstituted in DTCs. After PrV infection, results showed electrolyte regulation dysfunction in Na reabsorption for the disordered Na transporters, the broken reabsorption barrier, and the transformed microvilli. And it would lead to virus induced serum electrolyte abnormalities. This work brought us a new cognition about the advantages of organ-on-a-chip (OOC) in virus research, for it had given us a better insight into the pathogenesis of virus induced dysfunctions, based on its unique ability in function reproduction.

10.
ACS Nano ; 13(8): 9067-9073, 2019 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-31339690

RESUMO

Lithium-sulfur (Li-S) batteries have been explored extensively for high-capacity electric-power storage, but their practical application has been prevented by severe issues stemming from the use of a lithium anode and an organic-liquid electrolyte in which Li2Sx intermediates of the cell discharge reaction are soluble and shuttle to the anode. Both problems are addressed using bis(4-nitrophenyl) carbonate as an additive in the organic-liquid electrolyte. The soluble Li2Sx polysulfides react with the additive to create insoluble polysulfides with a lithium byproduct; this byproduct reacts with the Li-metal anode to create an anode passivation layer that is a good Li+ conductor, which allows for safe and rapid plating/stripping of lithium metal with a low impedance.

11.
Plant Physiol ; 181(1): 142-160, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31300470

RESUMO

Plasmodesmata act as key elements in intercellular communication, coordinating processes related to plant growth, development, and responses to environmental stresses. While many of the developmental, biotic, and abiotic signals are primarily perceived at the plasma membrane (PM) by receptor proteins, plasmodesmata also cluster receptor-like activities; whether these two pathways interact is currently unknown. Here, we show that specific PM-located Leu-rich-repeat receptor-like-kinases, Qian Shou kinase (QSK1) and inflorescence meristem kinase2, which under optimal growth conditions are absent from plasmodesmata, rapidly relocate and cluster to the pores in response to osmotic stress. This process is remarkably fast, is not a general feature of PM-associated proteins, and is independent of sterol and sphingolipid membrane composition. Focusing on QSK1, previously reported to be involved in stress responses, we show that relocalization in response to mannitol depends on QSK1 phosphorylation. Loss-of-function mutation in QSK1 results in delayed lateral root (LR) development, and the mutant is affected in the root response to mannitol stress. Callose-mediated plasmodesmata regulation is known to regulate LR development. We found that callose levels are reduced in the qsk1 mutant background with a root phenotype resembling ectopic expression of PdBG1, an enzyme that degrades callose at the pores. Both the LR and callose phenotypes can be complemented by expression of wild-type and phosphomimic QSK1 variants, but not by phosphodead QSK1 mutant, which fails to relocalize at plasmodesmata. Together, the data indicate that reorganization of receptor-like-kinases to plasmodesmata is important for the regulation of callose and LR development as part of the plant response to osmotic stress.

12.
Int J Mol Sci ; 20(13)2019 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-31261891

RESUMO

Consecutive monoculture practice facilitates enrichment of rhizosphere pathogenic microorganisms and eventually leads to the emergence of replant disease. However, little is known about the interaction relationship among pathogens enriched in rhizosphere soils, Nucleotide binding-leucine-rich repeats (NB-LRR) receptors that specifically recognize pathogens in effector-triggered immunity (ETI) and physiological indicators under replant disease stress in Rehmannia glutinosa. In this study, a controlled experiment was performed using different kinds of soils from sites never planted R. glutinosa (NP), replanted R. glutinosa (TP) and mixed by different ration of TP soils (1/3TP and 2/3TP), respectively. As a result, different levels of TP significantly promoted the proliferation of Fusarium oxysporum f.sp. R. glutinosa (FO). Simultaneously, a comparison between FO numbers and NB-LRR expressions indicated that NB-LRRs were not consecutively responsive to the FO proliferation at transcriptional levels. Further analysis found that NB-LRRs responded to FO invasion with a typical phenomenon of "promotion in low concentration and suppression in high concentration", and 6 NB-LRRs were identified as candidates for responding R. glutinosa replant disease. Furthermore, four critical hormones of salicylic acid (SA), jasmonic acid (JA), ethylene (ET) and abscisic acid (ABA) had higher levels in 1/3TP, 2/3TP and TP than those in NP. Additionally, increasing extents of SA contents have significantly negative trends with FO changes, which implied that SA might be inhibited by FO in replanted R. glutinosa. Concomitantly, the physiological indexes reacted alters of cellular process regulated by NB-LRR were affected by complex replant disease stresses and exhibited strong fluctuations, leading to the death of R. glutinosa. These findings provide important insights and clues into further revealing the mechanism of R. glutinosa replant disease.

13.
Biomater Sci ; 7(9): 3552-3565, 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31313767

RESUMO

Extracellular vesicles (EVs) are shed from most mammalian cells by different processes. EVs possess several distinct advantages, including excellent biocompatibility, good bio-stability and low immunogenicity. Moreover, they play significant roles in physiological and pathological processes. Challenges in EV research mainly concern highly efficient isolation, specific membrane surface engineering and further development of EV applications in biomedical fields. In this review, we summarize the recent and representative research regarding isolation, engineering and biomedical applications of EVs, which represent important research focus areas. These three aspects have not ever been systematically classified and summarized in previous reviews. Finally, we give our insights into the key issues concerning EVs and their future development for biomedical applications.

14.
Sensors (Basel) ; 19(13)2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31266216

RESUMO

Excellent performance, real-time and low memory requirement are three vital requirements for target detection in high resolution marine radar system. Unfortunately, many current state-of-the-art methods merely achieve excellent performance when coping with highly complex scenes. In fact, a common problem is that real-time processing, low memory requirement and remarkable detection ability are difficult to coordinate. To address this issue, we propose a novel detection framework which bases its principle on sampling and spatiotemporal detection. The framework consists of two stages, coarse detection and fine detection. Sampling-based coarse detection is designed to guarantee the real-time processing and low memory requirements by locating the area where targets may exist in advance. Different from former detection methods, multi-scan video data are utilized. In the stage of fine detection, the candidate areas are grouped into three categories: single target, dense targets and sea clutter. Different approaches for processing the different categories are implemented to achieve excellent performance. The superiority of the proposed framework beyond state-of-the-art baselines is well substantiated in this work. Low memory requirement of the proposed framework was verified by theoretical analysis. Real-time processing capability was verified by the video data of two real scenarios. Synthetic data were tested to show the improvement in tracking performance by using the proposed detection framework.

15.
Scand J Surg ; : 1457496919863886, 2019 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-31282314

RESUMO

OBJECTIVE: This study sought to investigate the relationship between ABO blood groups and the risk of gastric cancer as well as clinical pathological parameters and prognosis. METHODS: Gastric cancer patient data were collected from January 1995 to January 2012 at Jilin Cancer Hospital, and the blood group information of the blood donors at Jilin City Blood Center was recorded. The relationships between ABO blood group and both clinicopathological parameters and the risk of gastric cancer were analyzed retrospectively. The impact of ABO blood type on the 5-year survival rate of patients with gastric cancer was evaluated through outpatient and telephone interviews. RESULTS: (1) Compared with the healthy population, the frequency distribution of gastric cancer patients with the A blood group was significantly increased (χ2 = 4.708, P = 0.000), whereas the frequency distribution of gastric cancer patients with the AB blood group was significantly decreased (χ2 = 9.630, P = 0.002). However, there was no significant difference in the distributions of the B blood group and O blood group (P > 0.05). (2) The risk of gastric cancer in people with the A blood group was higher, whereas the risk of gastric cancer in people with the AB blood group was lower. There was no significant difference in the risk of gastric cancer between type B and type O patients (P > 0.05). (3) The ABO blood group was not related to pathological factors, including the size of the gastric tumor or the T stage or N stage of the disease (P > 0.05). (4) Univariate analysis results showed that the degree of differentiation, tumor size, T stage, lymph node metastasis, and type O blood were factors affecting the 5-year survival rate of gastric cancer patients (P < 0.05). Multivariate analysis results showed that tumor size, T stage, lymph node metastasis, and O blood group were independent prognostic factors. The 5-year survival rate for gastric cancer was significantly better in patients with type O blood (hazard ratio = 0.97, 95% confidence interval = 1.67-3.92). CONCLUSION: (1) The risk of gastric cancer was higher in patients with the A blood group and lower in those with the AB blood group. (2) The ABO blood group showed no significant effect on the clinicopathological parameters of gastric cancer. (3) The O blood group may be a prognostic factor for gastric cancer patients.

16.
Mol Cell Proteomics ; 18(8): 1556-1571, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31147492

RESUMO

Sucrose as a product of photosynthesis is the major carbohydrate translocated from photosynthetic leaves to growing nonphotosynthetic organs such as roots and seeds. These growing tissues, besides carbohydrate supply, require uptake of water through aquaporins to enhance cell expansion during growth. Previous work revealed Sucrose Induced Receptor Kinase, SIRK1, to control aquaporin activity via phosphorylation in response to external sucrose stimulation. Here, we present the regulatory role of AT3G02880 (QSK1), a receptor kinase with a short external domain, in modulation of SIRK1 activity. Our results suggest that SIRK1 autophosphorylates at Ser-744 after sucrose treatment. Autophosphorylated SIRK1 then interacts with and transphosphorylates QSK1 and QSK2. Upon interaction with QSK1, SIRK1 phosphorylates aquaporins at their regulatory C-terminal phosphorylation sites. Consequently, in root protoplast swelling assays, the qsk1qsk2 mutant showed reduced water influx rates under iso-osmotic sucrose stimulation, confirming an involvement in the same signaling pathway as the receptor kinase SIRK1. Large-scale phosphoproteomics comparing single mutant sirk1, qsk1, and double mutant sirk1 qsk1 revealed that aquaporins were regulated by phosphorylation depending on an activated receptor kinase complex of SIRK1, as well as QSK1. QSK1 thereby acts as a coreceptor stabilizing and enhancing SIRK1 activity and recruiting substrate proteins, such as aquaporins.

17.
J Agric Food Chem ; 67(28): 7954-7960, 2019 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-31199635

RESUMO

Phosphatidylethanolamine (PE) and phosphatidylserine (PS) have been shown to increase the antioxidant activity of α-tocopherol. This study investigated the ability of PE or PS to increase the antioxidant activity of different tocopherol homologues in bulk oil. In addition, the ability of a phospholipase-D-modified lecithin (high in PE) to increase the activity of α-tocopherol was determined. Results showed that PE was much more effective than PS at increasing the activity of the tocopherol homologues. The combination of mixed tocopherols with PE presented the greatest increase in antioxidant activity, with hydroperoxides and hexanal lag phases increasing 54 and 53 days compared to the mixed tocopherols alone. Phospholipase-D-modified lecithin increased the antioxidant activity of α-tocopherol in stripped bulk oil as well as a commercially refined oil with no added tocopherols. The study indicates that PE is a powerful tool to increase the antioxidant activity of tocopherols in bulk oil and that modification of lecithin to increase the PE concentration could be a commercially viable option to functionalize lecithin, so that its ability to inhibit lipid oxidation increases in bulk oils.


Assuntos
Lecitinas/química , Fosfolipase D/química , Fosfolipídeos/química , Óleo de Soja/química , Tocoferóis/química , Antioxidantes/química , Biocatálise , Aditivos Alimentares/química , Proteínas Fúngicas/química , Temperatura Alta , Oxirredução , Streptomyces/enzimologia
18.
J Agric Food Chem ; 67(26): 7249-7257, 2019 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-31244201

RESUMO

The duration of the rice growth phase has always been an important target trait. The identification of mutations in rice that alter these processes and result in a shorter growth phase could have potential benefits for crop production. In this study, we isolated an early aging rice mutant, pe-1, with light green leaves, using γ-mutated indica rice cultivar and subsequent screening methods, which is known as the phytochrome synthesis factor Se5 that controls rice flowering. The pe-1 plant is accompanied by a decreased chlorophyll content, an enhanced photosynthesis, and a decreased pollen fertility. PE-1, a close homologue of HY1, is localized in the chloroplast. Expression pattern analysis indicated that PE-1 was mainly expressed in roots, stems, leaves, leaf sheaths, and young panicles. The knockout of PE-1 using the CRISPR/Cas9 system decreased the chlorophyll content and downregulated the expression of PE-1-related genes. Furthermore, the chloroplasts of pe-1 were filled with many large-sized starch grains, and the number of osmiophilic granules (a chloroplast lipid reservoir) was significantly decreased. Altogether, our findings suggest that PE-1 functions as a master regulator to mediate in chlorophyll biosynthesis and photosynthetic pathways.


Assuntos
Cloroplastos/metabolismo , Heme Oxigenase-1/metabolismo , Oryza/enzimologia , Oryza/crescimento & desenvolvimento , Proteínas de Plantas/metabolismo , Clorofila/metabolismo , Cloroplastos/genética , Regulação da Expressão Gênica de Plantas , Heme Oxigenase-1/genética , Mutação , Oryza/genética , Oryza/metabolismo , Fotossíntese , Proteínas de Plantas/genética
19.
Acta Neurol Scand ; 140(4): 268-273, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31190374

RESUMO

OBJECTIVE: This study primarily aims to explore the value of combining the measurement of plasma α-synuclein oligomer levels with enhanced T2 star-weighted angiography (ESWAN) in the early diagnosis of Parkinson's disease. METHODS: Sixty patients with early Parkinson's disease and 30 normal adults, with similar ages and genders, were enrolled in the study. Their levels of plasma α-synuclein oligomers were measured, and ESWAN was performed. The amplitudes, phases and R2* values of the head, body and tail of the ipsilateral and contralateral substantia nigra pars compacta (SNc) were measured, at the side of the limb with severe symptoms or early symptoms. The receiver operating characteristic (ROC) curve was used to explore the value of these indexes in the early diagnosis of Parkinson's disease. RESULTS: The plasma level of α-synuclein oligomer was significantly higher in the experimental group than in the control group (P < 0.05). The amplitude values of the head and tail of contralateral SNcs were significantly lower in the experimental group than in the control group (P < 0.05). In the single-index assessment, the serum α-synuclein oligomer had the highest specificity (70%), while the sensitivity of the amplitude of the head and tail of the contralateral SNc was 75% and 80%, respectively. The area under the curve, for the combination of these three indicators, was 0.827, diagnostic efficiency was particularly high, and sensitivity and specificity both reached 80%. CONCLUSION: The combined detection of plasma α-synuclein oligomer and amplitude of the head and tail of the SNc has high diagnostic specificity and sensitivity.

20.
Appl Opt ; 58(17): 4812-4822, 2019 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-31251306

RESUMO

Due to the insufficient use of local information, the traditional fuzzy C-means (FCM) algorithm and its extension algorithm combined with spatial information show poor robustness and low segmentation accuracy. In addition, in the process of image segmentation based on the FCM algorithm, the initial center estimation is regarded as the process of searching the appropriate value in the gray range. To solve these problems, a new robust algorithm is proposed in this paper. The algorithm searches the optimal initial center by introducing an improved parallel Lévy grey wolf optimization algorithm, which is an improved fuzzy C-means segmentation algorithm that combines local information and adaptive gray weighting. Experimental results infer that both the precision and efficiency of the proposed method are superior to those of the state-of-arts.

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