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1.
Sci Total Environ ; 786: 147418, 2021 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-33975110

RESUMO

Links between the optical properties and chemical compositions of brown carbon (BrC) are poorly understood because of the complexity of BrC chromophores. We conducted field studies simultaneously at both vehicle-influenced site and biomass burning-affected site in China in polluted winter. The chemical compositions and light absorption values of functionalized aromatic compounds, including phenyl aldehyde, phenyl acid, and nitroaromatic compounds, were measured. P-phthalic acid, nitrophenols and nitrocatechols were dominant BrC species, accounting for over 50% of the concentration of identified chromophores. Nitrophenols and nitrocatechols contributed more than 50% of the identified BrC absorbance between 300 and 400 nm. Oxidation of biomass burning-related products (e.g., pyrocatechol and methylcatechols) and anthropogenic volatile organic compounds (e.g., benzene and toluene) generated similar BrC chromophores, implying that these functionalized aromatic compounds play an important role in both environments. Compared with the biomass burning-affected site (22%), functionalized aromatic compounds at vehicle-influenced site accounted for a higher percentage of BrC absorption (25%). This research improves our understanding of the links between optical properties and composition of BrC, and the difference between BrC chromophores from BB-influenced area and vehicle-affected area under polluted atmospheric conditions.

2.
Artigo em Inglês | MEDLINE | ID: mdl-33891388

RESUMO

Development of effective, environmentally friendly, facile large-scale processing, and low-cost materials is critical for renewable energy production. Here, MnOx/biochar composites were synthesized by a simple pyrolysis method and showed high performance for salinity gradient (SG) energy harvest in concentration flow cells (CFCs). The peak power density of CFCs with MnOx/biochar electrodes was up to 5.67 W m-2 (ave. = 0.91 W m-2) and stabilized for 500 cycles when using 1 and 30 g L-1 NaCl, which was attributed to their high specific capacitances and low electrode resistances. This power output was higher than all other reported MnO2 electrodes for SG energy harvest due to the synergistic effects between MnOx and biochar. When using a mixture with a molar fraction of 90% NaCl and 10% KCl (or Na2SO4, MgCl2, MgSO4, and CaCl2) in both feed solutions, the peak power density decreased by 2.3-40.1% compared to 100% NaCl solution with Ca2+ and Mg2+ showing the most pronounced negative effects. Our results demonstrated that the facile designed MnOx/biochar composite can be used for efficient SG energy recovery in CFCs with good stability, low cost, and less environmental impacts. When using natural waters as the feed solutions, pretreatment would be needed.

3.
Pharm Res ; 38(4): 669-680, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33796952

RESUMO

PURPOSE: To address the issue of local drug delivery in tumor treatment, a novel nanoparticle-hydrogel superstructure, namely semi-interpenetrating polymer networks (semi-IPNs) hydrogel composed of poly (ethylene glycol) diacrylate (PEGDA) and hyaluronic acid (HA) and incorporated with paclitaxel (PTX) loaded PLGA nanoparticles (PEGDA-HA/PLGA-PTX), was prepared by in situ UV photopolymerization for the use of local drug delivery. METHODS: Using the gelation time, swelling rate and degradation rate as indicators, the optimal proportion of Irgacure 2959 initiator and the concentration of HA was screened and obtained for preparing hydrogels. Next, paclitaxel (PTX) loaded PLGA nanoparticles (PLGA-PTX NPs) were prepared by the emulsion solvent evaporation method. RESULTS: The mass ratio of the initiator was 1%, and the best concentration of HA was 5 mg/mL in PEGDA-HA hydrogel. In vitro experiments showed that PLGA-PTX NPs had similar cytotoxicity to free PTX, and the cell uptake ratio on NCI-H460 cells was up to 96% by laser confocal microscopy and flow cytometry. The drug release of the PEGDA-HA/PLGA-PTX hydrogel local drug delivery system could last for 13 days. In vivo experiments proved that PEGDAHA/PLGA-PTX hydrogel could effectively inhibit the tumor growth without causing toxic effects in mice. CONCLUSIONS: This study demonstrated that the PEGDA-HA/PLGA-PTX hydrogel is a promising local drug delivery system in future clinical applications for tumor therapy. A photopolymerized semi-interpenetrating polymer networks-based hydrogel incorporated with paclitaxel-loaded nanoparticles was fabricated by in situ UV photopolymerization, providing a promised nanoplatform for local chemotherapy of tumors.

4.
Zhongguo Gu Shang ; 34(4): 321-7, 2021 Apr 25.
Artigo em Chinês | MEDLINE | ID: mdl-33896129

RESUMO

OBJECTIVE: To explore the treatment strategy and clinical efficacy for os odontoideum complicated with atlantoaxial dislocation. METHODS: The clinical data of 17 patients with os odontoideum complicated with atlantoaxial dislocation surgically treated from January 2006 to January 2015 were retrospectively analyzed, including 7 males and 10 females, aged 17 to 53 (43.1±11.3) years old;course of disease was 3 to 27(10.2±6.9) months. All patients received cranial traction before operation, 12 of 14 patients with reducible dislocation were treated by posterior atlantoaxial fixation and fusion, and 2 patients with atlantooccipital deformity were treated by posterior occipitocervical fixation and fusion;3 patients with irreducible alantoaxial dislocation were treated by transoral approach decompression combined with posterior atlantoaxial fixation and fusion. The operation time, intraoperative blood loss and perioperative complications were recorded. Visual analogue scale (VAS) and Japanese Orthopaedic Association (JOA) score were used to evaluate the change of neck pain and neurological function. Atlantoaxial joint fusion rate was evaluated by CT scan. RESULTS: The operation time of posterior fixation and fusion ranged from 86 to 170 (92.2±27.5) min, and the intraoperative blood loss was 200-350 (250.7±65.2) ml. No vertebral artery injury and spinal cord injury were recorded. Among the patients underwent atlantoaxial fixation and fusion, 1 patient with reducible dislocation fixed by C2 laminar screw lost reduction after primary operation, and received anterior release again and finally occipitocervical fusion. All patients were followed up for 15 to 58 (32.0±12.2) months. VAS score was decreased from preoperative 4.2±0.9 to 1.3±0.7 at final follow up and the JOA score was improved from preoperative 11.2±1.2 to 16.9±0.8 at final follow-up. CT scan confirmed that the atlantoaxial or occipitocervical fusion wasgood, and the fusion time was 5 to 9 (6.7±0.6) months. CONCLUSION: Surgical treatment of os odontoideum complicated with atlantoaxial dislocation can achieve satisfactory results, improve the patient's neurological function and improve the quality of life, however the surgical options needs to be individualized.


Assuntos
Articulação Atlantoaxial , Vértebra Cervical Áxis , Luxações Articulares , Fusão Vertebral , Adolescente , Adulto , Articulação Atlantoaxial/diagnóstico por imagem , Articulação Atlantoaxial/cirurgia , Feminino , Humanos , Luxações Articulares/cirurgia , Masculino , Pessoa de Meia-Idade , Qualidade de Vida , Estudos Retrospectivos , Resultado do Tratamento , Adulto Jovem
5.
Huan Jing Ke Xue ; 42(5): 2101-2109, 2021 May 08.
Artigo em Chinês | MEDLINE | ID: mdl-33884779

RESUMO

To explore the concentrations, characteristics, and sources of organic aerosols in winter in Beijing, atmospheric fine particulate matter (PM2.5) samples were collected from November 10, 2016 to December 10, 2016. One hundred and twenty-nine particulate organic matters (POM) were quantified by gas chromatography-mass spectrometry, accounting for approximately 9.3%±1.2% of the total concentration of organic matter. The most abundant class was sugar, among which levoglucosan alone accounted for 18% of the quantified organic matter mass. The next most abundant classes were alkanoic acids, normal alkanes, dicarboxylic acids, and polycyclic aromatic hydrocarbons. The influence of winter heating and biomass burning emissions on organic aerosols in winter in Beijing was analyzed by the characteristics of the molecular markers in the POM. Compared with those during the non-heating period, the concentrations and proportions of hopane species, which are tracers for fossil fuels, increased in the organic matters during the heating period. Moreover, the influence of coal burning emissions on the distribution of hopane species was enhanced. The species with the maximum concentration and carbon predominance index in n-alkanes also reflected the influence of enhanced fossil fuel emissions. The results of the concentration-weighted trajectory model for levoglucosan, a tracer for biomass combustion, suggested that straw burning pollution in the surrounding areas of Beijing would affect the composition of organic aerosols in Beijing via airmass transport. A molecular marker-based chemical mass balance model was used to apportion the sources of organic carbon in the winter of 2016 in Beijing, and the results were compared with those of research in 2006 to quantify the changes in the source contributions over 10 years. The contribution of motor vehicles increased significantly in 2016 compared with that in 2006, whereas the contribution of coal burning and wood burning decreased to a large extent. The contribution of cooking emissions could not be ignored. Therefore, the control of motor vehicle and cooking emissions is of great importance to reduce the problem of PM2.5 pollution in winter in Beijing.

6.
Acta Physiol (Oxf) ; 231(4): e13595, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33835704

RESUMO

AIMS: In patients with renal disease, high serum phosphate shows a relationship with cardiovascular risk. We speculate that high phosphate (HP) impairs arterial vasodilation via the endothelium and explore potential underlying mechanisms. METHODS: Isolated vessel relaxation, endothelial function, glomerular filtration rate (GFR), oxidative stress status and protein expression were assessed in HP diet mice. Mitochondrial function and protein expression were assessed in HP-treated human umbilical vein endothelial cells (HUVECs). RESULTS: High phosphate (1.3%) diet for 12 weeks impaired endothelium-dependent relaxation in mesenteric arteries, kidney interlobar arteries and afferent arterioles; reduced GFR and the blood pressure responses to acute administration of acetylcholine. The PPARα/LKB1/AMPK/eNOS pathway was attenuated in the endothelium of mesenteric arteries from HP diet mice. The observed vasodilatory impairment of mesenteric arteries was ameliorated by PPARα agonist WY-14643. The phosphate transporter PiT-1 knockdown prevented HP-mediated suppression of eNOS activity by impeding phosphorus influx in HUVECs. Endothelium cytoplasmic and mitochondrial reactive oxygen species (ROS) were increased in HP diet mice. Moreover HP decreased the expression of mitochondrial-related antioxidant genes. Finally, mitochondrial membrane potential and PGC-1α expression were reduced by HP treatment in HUVECs, which was partly restored by AMPKα agonist. CONCLUSIONS: HP impairs endothelial function by reducing NO bioavailability via decreasing eNOS activity and increasing mitochondrial ROS, in which the AMPK-related signalling pathways may play a key role.

7.
Cancer Res ; 2021 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-33687950

RESUMO

TNF Receptor Apoptosis-Inducing Ligand (TRAIL) can activate cell surface death receptors resulting in potent tumor cell death via induction of the extrinsic apoptosis pathway. Eftozanermin alfa (ABBV-621) is a second-generation TRAIL receptor agonist engineered as an IgG1-Fc mutant backbone linked to two sets of trimeric native single chain TRAIL receptor binding domain monomers. This hexavalent agonistic fusion protein binds to the death-inducing DR4 and DR5 receptors with nanomolar affinity to drive on-target biological activity with enhanced caspase-8 aggregation and DISC formation independent of FcγR-mediated cross-linking, and without clinical signs or pathological evidence of toxicity in non-rodent species. ABBV-621 induced cell death in approximately 36% (45/126) of solid cancer cell lines in vitro at sub-nanomolar concentrations. An in vivo patient-derived xenograft (PDX) screen of ABBV-621 activity across 15 different tumor indications resulted in an overall response (OR) of 29% (47/162). Although DR4 (TNFSFR10A) and/or DR5 (TNFSFR10B) expression levels did not predict the level of response to ABBV-621 activity in vivo, KRAS mutations were associated with elevated TNFSFR10A and TNFSFR10B and were enriched in ABBV-621 responsive colorectal carcinoma (CRC) PDX models. To build upon the OR of ABBV-621 monotherapy in CRC (45%; 10/22) and pancreatic cancer (35%; 7/20), we subsequently demonstrated that inherent resistance to ABBV-621 treatment could be overcome in combination with chemotherapeutics or with selective inhibitors of BCL-XL. In summary, these data provide a pre-clinical rationale for the ongoing Phase-1 clinical trial (NCT03082209) evaluating the activity of ABBV-621 in cancer patients.

8.
Zhongguo Gu Shang ; 34(3): 228-34, 2021 Mar 25.
Artigo em Chinês | MEDLINE | ID: mdl-33787166

RESUMO

OBJECTIVE: To investigate the clinical efficacy and superiority of direct lateral interbody fusion combined with posterior percutaneous screw fixation in the treatment of lumbar tuberculosis. METHODS: From June 2013 to August 2016, the clinical data of 83 patients with lumbar tuberculosis were retrospectively analyzed, including 55 males and 28 females, aged from 27 to 72 (49.5±13.5) years. These 83 patients were divided into two groups according to different operation methods, 35 cases in group A were treated with direct lateral interbody fusion combined with posterior percutaneous screw fixation;48 cases in group B were treated with anterior traditional extraperitoneal debridement combined with posterior internal fixation. After operation, regular quadruple antituberculosis drugs were continued for 18 months. The operation time, intraoperative blood loss, hospital stay, bone graft fusion time and complications were compared between the two groups. Visual analogue score (VAS) of lumbar pain, Oswestry Disability Index (ODI), sagittal Cobb angle, erythrocyte sedimentation rate (ESR) and C-reactive protein(CRP) values before and after operation were analyzed. RESULTS: The operation was successfully completed in both groups, and the operation mode was not changed during operation. The operation time, intraoperative blood loss and hospital stay were (149.4±13.3) min, (354.3±69.0) ml, (9.4±1.6) d in group A and(116.8±10.0) min, (721.9±172.3) ml, (11.8±1.7) d in group B, respectively, with significant difference between the two groups (P<0.05). The follow up time was (24.2±5.1) months in group A and (24.0±5.0) months in group B, there was no significant difference between two groups (P>0.05). At the follow-up of 4 months after operation, one patient in group A was found to have enlarged psoas major abscess on the contralateral side, and was cured after secondary operation. No sinus formation, cerebrospinal fluid leakage, internal fixation loosening, fracture or distal junction kyphosis were found during follow-up. The fusion time was (5.1±1.6) months in group A and (5.1± 1.7) months in group B, there was no significant difference between two groups (P>0.05). The VAS, ODI score, sagittal Cobb angle, ESR and CRP value of the lesion segment at the last follow-up of the two groups were significantly improved (P<0.05), but there was no significant difference between two groups (P>0.05). CONCLUSION: The two kinds of operation can obtain satisfactory clinical effect. Direct lateral interbody fusion combined with posterior percutaneous screw fixation can reduce intraoperative blood loss and hospital stay, which is conducive to early rehabilitation of patients.


Assuntos
Parafusos Pediculares , Fusão Vertebral , Tuberculose da Coluna Vertebral , Idoso , Transplante Ósseo , Desbridamento , Feminino , Humanos , Vértebras Lombares/cirurgia , Masculino , Estudos Retrospectivos , Vértebras Torácicas , Resultado do Tratamento , Tuberculose da Coluna Vertebral/cirurgia
9.
Artigo em Inglês | MEDLINE | ID: mdl-33523329

RESUMO

Kashin-Beck disease (KBD) is an endemic disease in China with the highest incidence rate in Tibet region. Promoted generation of oxygen free radicals by semiquinone structure of humic substance (HS) in drinking water was considered to be one of its pathogeneses. Therefore, detailed analysis of HS was performed in water and sediment samples collected from three endemic and three disease-free areas in Changdu Region, Tibet, China. After purification of the HS in the samples, the fractions of HS were characterized using electron paramagnetic resonance, 13C nuclear magnetic resonance, fluorescence spectroscopy with parallel factor analysis and Fourier transform infrared spectroscopy (FTIR). The organic carbon content of HS did not show a significant difference between endemic and disease-free areas or correlation with KBD-associated morbidity. Except FTIR, all techniques succeeded in characterization of the quinone redox system, indicating their validity and consistency. The quinone redox system in aquatic HS exhibited significantly higher level of the following indexes in endemic areas than disease-free areas: semiquinone radical content of fulvic acid (FA) (p < 0.05), aromaticity of FA (p < 0.05), fluorescence intensity (per gram carbon) of reduced quinone-like component of FA (p < 0.05) and humic acid (HA) (p < 0.1). Semiquinone radical content (r = 0.781, p < 0.1), aromaticity of FA (r = 0.891, p < 0.05), intensity of oxidized quinone-like component (r = 0.875, p < 0.05) and reduced quinone-like component of FA (r = 0.793 p < 0.1) showed medium to strong correlation with KBD-associated morbidity. Generally, the content of reduced quinone and aquatic FA showed stronger differences between endemic and disease-free areas than oxidized quinone and aquatic HA, respectively. The quinone redox system in sediment HS did not show any significant relationship with KBD. The present study is a successful attempt to combine the three indexes, semiquinone radical content, aromaticity and fluorescence intensity, in characterizing quinone redox system in HS, facilitating more comprehensive understanding of the characteristics of HS in KBD-affected regions.

10.
Environ Pollut ; 276: 116723, 2021 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-33611207

RESUMO

With increasing concerns on the ecological risks of pollutants, many efforts have been devoted to revealing the toxic effects of pollutants on algae or bacteria in their monocultures. However, how pollutants affect algae and bacteria in their cocultures is still elusive but crucial due to its more environmental relevance. The present review outlines the interactions between algae and bacteria, reveals the influential mechanisms of pollutants (including pesticides, metals, engineered nanomaterials, pharmaceutical and personal care products, and aromatic pollutants) to algae and bacteria in their coexisted systems, and puts forward prospects for further advancing toxic studies in algal-bacterial systems. Pollutants affect the physiological and ecological functions of bacteria and algae by interfering with their relationships. Cell-to-cell adhesion, substrate exchange and biodegradation of organic pollutants, enhancement of signal transduction, and horizontal transfer of tolerance genes are important defense strategies in algal-bacterial systems to cope with pollution stress. Developing suitable algal-bacterial models, identifying cross-kingdom signaling molecules, and deciphering the horizontal transfer of pollutant resistant genes between algae and bacteria under pollution stress are the way forward to fully exploit the risks of pollutants in natural aquatic environments.


Assuntos
Poluentes Ambientais , Praguicidas , Poluentes Químicos da Água , Bactérias/genética , Biodegradação Ambiental , Poluentes Químicos da Água/toxicidade
11.
J Ethnopharmacol ; 271: 113886, 2021 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-33524513

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Huangqin Decoction (HQD), a traditional Chinese medicinal (TCM) formula chronicled in Shang Han Lun, has been used to treat gastrointestinal diseases for nearly 1800 years. OBJECTIVE: To investigate the effects and underlying mechanisms of HQD on ulcerative colitis (UC). METHODS: The bioactive compounds in HQD were obtained from the traditional Chinese medicine systems pharmacology database. Then, the HQD and UC-related targets were analyzed by establishing HQD-Compounds-Targets (H-C-T) and protein-protein interaction (PPI) networks. Enrichment analysis was used for further study. The candidate targets for the effects of HQD on UC were validated using a dextran sulfate sodium-induced UC mouse experiment. RESULTS: The results showed that 51 key targets were gained by matching 284 HQD-related targets and 837 UC-related targets. Combined with H-C-T and PPI network analyses, the key targets were divided into endothelial growth, inflammation and signal transcription-related targets. Further experimental validation showed that HQD targeted estrogen receptor alpha (ESR1) and endothelial growth factor receptors to relieve endothelial dysfunction, thereby improving intestinal barrier function. The expression of inflammatory cytokines and signal transducers was suppressed by HQD treatment and inflammation was inhibited. CONCLUSIONS: HQD may acts on UC via the regulation of targets and pathways related to improving the intestinal mucosal barrier and ameliorating endothelial dysfunction. Additionally, ERS1 may be a new target to explore the mechanisms of UC.

12.
Mol Plant Pathol ; 22(3): 348-360, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33433944

RESUMO

The plant pathogen Agrobacterium tumefaciens causes crown gall disease and is a widely used tool for generating transgenic plants owing to its virulence. The pathogenic process involves a shift from an independent to a living form within a host plant. However, comprehensive analyses of metabolites, genes, and reactions contributing to this complex process are lacking. To gain new insights about the pathogenicity from the viewpoints of physiology and cellular metabolism, a genome-scale metabolic model (GSMM) was reconstructed for A. tumefaciens. The model, referred to as iNX1344, contained 1,344 genes, 1,441 reactions, and 1,106 metabolites. It was validated by analyses of in silico cell growth on 39 unique carbon or nitrogen sources and the flux distribution of carbon metabolism. A. tumefaciens metabolic characteristics under three ecological niches were modelled. A high capacity to access and metabolize nutrients is more important for rhizosphere colonization than in the soil, and substantial metabolic changes were detected during the shift from the rhizosphere to tumour environments. Furthermore, by integrating transcriptome data for tumour conditions, significant alterations in central metabolic pathways and secondary metabolite metabolism were identified. Overall, the GSMM and constraint-based analysis could decode the physiological and metabolic features of A. tumefaciens as well as interspecific interactions with hosts, thereby improving our understanding of host adaptation and infection mechanisms.

13.
J Hazard Mater ; 411: 125042, 2021 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-33429307

RESUMO

Both green tea (GT) extract and vitamin C (VC) were used for the reduction of Fe3+ to Fe0 using a green synthesis method. Modified nanozero-valent iron (GT-nZVI@VC nanocomposites) was successfully obtained and characterized as α-Fe0-iron oxide/VC by multiple analytical methods. The GT-nZVI@VC nanocomposites showed better transportability than nZVI, in that transport behavior was slightly dependent on various ratios of sand/soil in water-saturated heterogeneous porous media. Breakthrough curves of GT-nZVI@VC nanocomposites in paddy soil exhibited "blocking effects" and were well described using a first-order straining coefficient (k2) on site 2 obtained from a two-site kinetic attachment model. In particular, GT-nZVI@VC (VC/Fe = 0.6) showed higher Cr(VI) removal (especially reducibility) in both paddy soil and water compared to that of nZVI and VC. It is likely that the synergistic effects of VC (ascorbic acid) and tea polyphenols can increase the released free electrons into solution, favoring the high reduction of Cr(VI) into Cr(III) (i.e., FeOCr2O3, Cr(OH)3 and Cr2O3), where Cr(III) is prone to be immobilized by the nanocomposites in soil. This research highlights that VC can mediate the activation of GT extract to successfully modify nZVI, which could be beneficial for efficient transport in subsurface and remediation of Cr(VI)-contaminated soil and underground water.

14.
Ecotoxicol Environ Saf ; 208: 111615, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33396135

RESUMO

Thyroid tumor and thyroid goiter are prevalent disease around the world. In this case-control study, we investigated the association between exposure to a total of twelve mineral elements and thyroid disease as well as thyroid functions. Participants with thyroid tumor or goiter (N = 197) were matched with a healthy population (N = 197) by age (± 2 years old) and same sex. Questionnaires were used to collect data about the demographic characteristics and information of subjects. Serum and urine samples were collected simultaneously for each of the subjects. Mineral elements, iodine level of urine and levels of the total seven thyroid function indexes in serum were detected respectively. Conditional logistic regression was applied to estimate the associations between mineral elements and the risk of thyroid tumor and goiter through single-element models and multiple-element models. Multiple linear regression was used to evaluate relationships between mineral elements and percentage changes of thyroid functions. Higher concentrations of mineral elements in the recruited population were found in this study than other comparable studies, and the levels of chromium (Cr), manganese (Mn), nickel (Ni), arsenic (As), cadmium (Cd), selenium (Se), antimony (Sb), thallium (Tl) and lead (Pb) in the case group were lower than the control group. According to the single-element models, Cr, Mn, Ni, Sb and Tl showed significant negative associations with the risk of thyroid tumor and goiter, and, Cd showed nonmonotonic dose response. Cd and mercury (Hg) showed a nonmonotonic percentage change with T4, while Tl was associated with the increased FT4 in the control group. Therefore, Cd, Hg and Tl may disturb the balance of thyroid function to some extent, and Cr, Mn, Ni, Cd, Sb, and Tl may become potential influencing factors for the risk of thyroid tumor and goiter.


Assuntos
Bócio/metabolismo , Metais Pesados/metabolismo , Minerais/metabolismo , Glândula Tireoide/metabolismo , Neoplasias da Glândula Tireoide/metabolismo , Oligoelementos/metabolismo , Estudos de Casos e Controles , Pré-Escolar , Feminino , Bócio/epidemiologia , Bócio/urina , Humanos , Iodo/urina , Modelos Lineares , Masculino , Metais Pesados/urina , Minerais/urina , Análise Multivariada , Testes de Função Tireóidea , Neoplasias da Glândula Tireoide/epidemiologia , Neoplasias da Glândula Tireoide/urina , Oligoelementos/urina , Adulto Jovem
15.
Cancer Med ; 10(2): 563-574, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33356013

RESUMO

The low rate of durable response against relapsed and/or refractory multiple myeloma (RRMM) in recent studies indicates that chimeric antigen receptor T-cell (CART) treatment is yet to be optimized. This study aims to investigate the safety and efficacy of sequential infusion of CD19-CART and B-cell maturation antigen (BCMA)-CARTs for RRMM with a similar 3 + 3 dose escalation combined with a toxicity sentinel design. We enrolled 10 patients, among whom 7 received autologous infusion and 3 received allogeneic infusion. The median follow-up time was 20 months. The most common grade 3/4 treatment-emergent toxicities were hematological toxicities. Cytokine-release syndrome (CRS) adverse reactions were grade 1/2 in 9 out of 10 subjects. No dose-limited toxicity (DLT) was observed for BCMA-CAR-positive T cells ≤5 × 107 /kg), while two patients with dose-levels of 5-6.5 × 107 /kg experienced DLTs. The overall response rate was 90% (five partial responses and four stringent complete responses). Three out of four patients with stringent complete responses to autologous CART had progression-free survival for over 2 years. The three patients with allogeneic CART experienced disease progression within 2 months. These results evidence the sequential infusion's preliminarily tolerability and efficacy in RRMM, and present a simple and safe design applicable for the establishment of multiple CART therapy.

16.
Biomed Pharmacother ; 134: 111104, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33341045

RESUMO

Non-alcoholic fatty liver disease (NAFLD) has been a leading cause of chronic metabolic disease, seriously posing healthy burdens to the public, whereas interventions available for it are limited to date. Patchouli oil had been reported to attenuate hepatic steatosis in our previous study. ß-patchoulene (ß-PAE) is a representative component separated from patchouli oil with multiple activities, but its effect against NAFLD is still unknown. To investigate the effect and potential mechanism of ß-PAE on NAFLD, we used high fat diet (HFD) in vivo and free fatty acid (FFA) in vitro to induce hepatic steatosis in rats and L02 cells, respectively. Histological examination was evaluated via Hematoxylin-eosin and oil red O staining. The parameters for hepatic steatosis were estimated via biochemical kits, western blotting and quantitative real-time PCR. Compound C, the inhibitor of AMPK, was applied further to examine the precise mechanism of ß-PAE on NAFLD. Our results indicated that ß-PAE significantly attenuated HFD-induced weight gain, hepatic injury, lipid deposition in serum and hepatic tissue as well as FFA induced-lipid accumulation. Besides, ß-PAE markedly improved the expression of AMP-activated protein kinase (AMPK) and its downstream factors which correlate with hepatic lipid synthesis and oxidation in vivo and in vitro. Nevertheless, Compound C abrogated the benefits derived from ß-PAE in L02 cells. In conclusion, these results suggest that ß-PAE exerts AMPK agonist-like effect to regulate hepatic lipid synthesis and oxidation, eventually prevent NAFLD progression.

17.
J Nanosci Nanotechnol ; 21(2): 949-954, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33183429

RESUMO

The most common malignant tumor of the human endocrine system is thyroid cancer, most used surgical treatment for thyroid cancer is total thyroidectomy with central lymph node dissection. However, surgery and thorough lymph node dissection can easily damage the parathyroid glands and cause corresponding surgery. Symptoms such as permanent hyperparathyroidism, nano-carbon is a common type of new lymphoid tissue tracer, it is mainly used to trace lymphoid tissue in tumor surgery. With the continuous advancement of surgical technology, more and more scholars have used nano-carbon to trace lymphoid tissue during thyroid cancer surgery, and have achieved good results. The results of this study show that thyroid cancer surgery with nano-carbon to negatively develop the parathyroid glands significantly reduces the incidence of serum PTH and blood calcium decline, and largely protects the parathyroid glands. Practice has also shown that the use of nano-carbon tracers is more thorough in lymph node dissection than thyroid cancer surgery without nano-carbon tracers.

18.
Neuroimage ; 227: 117628, 2021 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-33316394

RESUMO

The macro-scale intrinsic functional network architecture of the human brain has been well characterized. Early studies revealed robust and enduring patterns of static connectivity, while more recent work has begun to explore the temporal dynamics of these large-scale brain networks. Little work to date has investigated directed connectivity within and between these networks, or the temporal patterns of afferent (input) and efferent (output) connections between network nodes. Leveraging a novel analytic approach, prediction correlation, we investigated the causal interactions within and between large-scale networks of the brain using resting state fMRI. This technique allows us to characterize information transfer between brain regions in both the spatial (direction) and temporal (duration) scales. Using data from the Human Connectome Project (N = 200) we applied prediction correlation techniques to four resting-state fMRI scans (each scan has TRs = 1200). Three central observations emerged. First, the strongest and longest duration connections were observed within the somatomotor, visual, and dorsal attention networks. Second, the short duration connections were observed for high-degree nodes in the visual and default networks, as well as in the hippocampus. Specifically, the connectivity profile of the highest-degree nodes was dominated by efferent connections to multiple cortical areas. Moderate high-degree nodes, particularly in hippocampal regions, showed an afferent connectivity profile. Finally, multimodal association nodes in lateral prefrontal brain regions demonstrated a short duration, bidirectional connectivity profile, consistent with this region's role in integrative and modulatory processing. These results provide novel insights into the spatiotemporal dynamics of human brain function.


Assuntos
Encéfalo/diagnóstico por imagem , Conectoma , Rede Nervosa/diagnóstico por imagem , Adulto , Mapeamento Encefálico/métodos , Feminino , Humanos , Imagem por Ressonância Magnética , Masculino , Descanso , Adulto Jovem
19.
Environ Pollut ; 267: 115671, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33254642

RESUMO

Perfluorooctane sulfonate (PFOS) potentially adsorbs on the surface of suspended sediment (SPS), which can develop a toxic "pool" bioavailable to benthic organisms. In this study, the freshwater clam Corbicula fluminea was employed as a zoobenthos model to study the effects of SPS (collected from the Yellow River) on the bioaccumulation and toxicity (from the molecular level to cellular and physiological levels) caused by PFOS exposure. Besides, the enhanced integrated biomarker response (EIBR) system was applied as an index to evaluate the in-depth toxic effects of PFOS and SPS single and co-exposure at various treatment levels. Our results demonstrated that PFOS-SPS co-exposure (at sub-lethal doses of PFOS) significantly increased the bioaccumulation of PFOS, and induced the elevated levels of reactive oxygen species (ROS), the significantly increased activities of superoxide dismutase (SOD) and catalase (CAT) enzymes, the significantly increased content of malondialdehyde (MDA), and the significantly upregulated expression levels of sod, selenium-dependent glutathione peroxidase (se-gpx), heat shock protein 22 (hsp22), heat shock protein 40 (hsp40) and cytochrome P450 30 (cyp30) genes. Further, the co-exposure induced the significantly higher histopathological alterations in the gonads and digestive glands, and even elevated the inhibition of siphoning behavior in clams. In addition, the EIBR index also revealed the highest values for PFOS and SPS co-exposure, compared to the individual SPS or PFOS exposure. The results indicated that at high levels of PFOS exposure (especially at 1000 µg/L), the presence of SPS might increase the generation of ROS by influencing the bioaccumulation of PFOS, which enhanced the toxicity of PFOS to C. fluminea. These results potentially provide basic information for the comprehensive evaluation of the toxic effects of PFOS on benthos in a multi-sediment river ecosystem.

20.
Water Res ; 190: 116710, 2020 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-33285452

RESUMO

Chlorination can lead to the formation of hazardous chlorinated disinfection byproducts (Cl-DBPs). We identified tyrosine (Tyr) and tryptophan (Trp) as precursors of toxic Cl-DBPs and developed a halogen extraction code to complement ultra performance liquid chromatography in tandem with high resolution mass spectrometry (UPLC-HRMS) in detecting and identifying Cl-DBPs. We detected 20 and 11 Cl-DBPs formed from chlorination of Tyr and Trp, respectively, and identified the structures of 15 Cl-DBPs. Fourteen structures were previously unreported. We also proposed the tentative formation pathways of these newly identified Cl-DBPs. Their incidence in real water sources demonstrated that these Cl-DBPs are likely to form during chlorination of reclaimed water. We computationally predicted the toxicity of these Cl-DBPs, which was relatively high, indicating that these Cl-DBPs could be hazardous and were of valid concern. Combining analytical data with the halogen extraction code can identify Cl-DBPs accurately from complex compounds. This analytical method can be used to identify Cl-DBPs of water treatment procedures in further studies.

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