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1.
Int J Biol Sci ; 18(2): 599-616, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35002512

RESUMO

Lactic acid (LA) metabolism in the tumor microenvironment contributes to the establishment and maintenance of immune tolerance. This pathway is characterized in tumor associated macrophages. However, the role and pathway of LA metabolism at maternal-fetal interface during early pregnancy, especially in decidual macrophage differentiation, are still unclear. Herein, for the first time, we discovered that LA can trigger either M2 or M1 macrophage polarization via oxidative phosphorylation and glycolysis regulation under normoxia or hypoxia, respectively. Also, LA metabolism played a vital role in decidual macrophages-mediated recurrent pregnancy loss (RPL), through HIF-1α/SRC/LDHA pathway. Moreover, blockade of LA intake with AZD3965 (MCT-1 inhibitor) could rescue pregnancy in an abortion-prone mouse model, suggesting a potential therapeutic target in RPL. Collectively, the present study identifies the previously unknown functions of LA metabolism in the differentiation of decidual macrophages in early normal pregnancy and RPL, and provides a potential therapeutic strategy in RPL by manipulating decidual macrophages' functions through LA metabolic pathway.

2.
Br Poult Sci ; 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-35000502

RESUMO

1. This study examined the transcriptomic profiles of contour and flight feather follicles from two duck breeds to determine the molecular network and the candidate genes associated with contour and flight feather morphogenesis.2. High-throughput RNA sequencing was performed to compare differences in feather follicles between contour and flight feathers in two duck breeds (Heiwu and Nonghua duck).3. Comparing the contour feather follicles with flight feather follicles, 4,757 and 4,820 differentially expressed genes (DEGs) were identified in Heiwu and Nonghua duck respectively. Weighted gene co-expression network analysis (WGCNA) was used to construct a gene co-expression network of all DEGs and identify the key modules and hub genes associated with feather morphogenesis.4. Two key modules were enriched in many pathways involved in feather morphogenesis, such as the Wnt signalling pathway, anatomical structure morphogenesis, and focal adhesion. The CCNA2, TTK, NUF2, ECT2 and INCENP (in one module), and PRSS23, LAMC1, IGFBP3, SHISA5, and APLP2 (in another module) may be essential candidate genes for influencing feather morphology. Moreover, seven transcription factors (TFs) (UBP1, MBD2, ZNF512B, SMAD1, CAPN15, JDP2, KLF10, and MEF2A) were predicted to regulate the essential genes that contribute to feather morphogenesis.5. This work demonstrated gene expression changes of contour and flight feather follicles and is beneficial for further understanding of the complex structure of feathers.

3.
J Exp Clin Cancer Res ; 41(1): 15, 2022 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-34998404

RESUMO

BACKGROUND: Acquired resistance of 5-fluorouracil (5-FU) remains a clinical challenge in colorectal cancer (CRC), and efforts to develop targeted agents to reduce resistance have not yielded success. Metabolic reprogramming is a key cancer hallmark and confers several tumor phenotypes including chemoresistance. Glucose metabolic reprogramming events of 5-FU resistance in CRC has not been evaluated, and whether abnormal glucose metabolism could impart 5-FU resistance in CRC is also poorly defined. METHODS: Three separate acquired 5-FU resistance CRC cell line models were generated, and glucose metabolism was assessed by measuring glucose and lactate utilization, RNA and protein expressions of glucose metabolism-related enzymes and changes of intermediate metabolites of glucose metabolite pool. The protein levels of hypoxia inducible factor 1α (HIF-1α) in primary tumors and circulating tumor cells of CRC patients were detected by immunohistochemistry and immunofluorescence. Stable HIF1A knockdown in cell models was established with a lentiviral system. The influence of both HIF1A gene knockdown and pharmacological inhibition on 5-FU resistance in CRC was evaluated in cell models in vivo and in vitro. RESULTS: The abnormality of glucose metabolism in 5-FU-resistant CRC were described in detail. The enhanced glycolysis and pentose phosphate pathway in CRC were associated with increased HIF-1α expression. HIF-1α-induced glucose metabolic reprogramming imparted 5-FU resistance in CRC. HIF-1α showed enhanced expression in 5-FU-resistant CRC cell lines and clinical specimens, and increased HIF-1α levels were associated with failure of fluorouracil analog-based chemotherapy in CRC patients and poor survival. Upregulation of HIF-1α in 5-FU-resistant CRC occurred through non-oxygen-dependent mechanisms of reactive oxygen species-mediated activation of PI3K/Akt signaling and aberrant activation of ß-catenin in the nucleus. Both HIF-1α gene knock-down and pharmacological inhibition restored the sensitivity of CRC to 5-FU. CONCLUSIONS: HIF-1α is a potential biomarker for 5-FU-resistant CRC, and targeting HIF-1a in combination with 5-FU may represent an effective therapeutic strategy in 5-FU-resistant CRC.

4.
J Am Acad Dermatol ; 2022 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-35026342

RESUMO

BACKGROUND: Vunakizumab (SHR-1314) is a novel interleukin-17A monoclonal antibody that has shown preliminary efficacy and tolerability in phase I trials. OBJECTIVE: To evaluate the efficacy and safety of vunakizumab in moderate-to-severe plaque psoriasis. METHODS: In this 36-week, multi-center, double-blinded, phase II study (NCT03463187), 187 eligible patients with moderate-to-severe plaque psoriasis were randomized 1:1:1:1:1 to receive vunakizumab (40, 80, 160, or 240 mg) or placebo subcutaneously every four weeks until week 12 (2 more drug administrations for the vunakizumab groups on week 16/20). The primary endpoint was at least 75% improvement in the psoriasis area and severity index (PASI 75) at week 12. RESULTS: At week 12, there were significantly greater proportions of PASI 75 responders in all vunakizumab groups compared to placebo (40, 80, 160, 240 mg: 56.8%, 65.8%, 81.6%, 86.5% vs 5.4%; all P < .001); the proportions of patients achieving physician's global assessment response of 0 or 1 were also higher with vunakizumab (45.9%, 47.4%, 60.5%, 73.0% vs 8.1%). No unexpected adverse effects were observed. LIMITATIONS: The study was relatively short in duration and included no active control. CONCLUSION: Vunakizumab showed promising efficacy for moderate-to-severe plaque psoriasis, with good tolerability, warranting further investigation in larger and longer-term studies.

5.
Food Chem ; 378: 132034, 2022 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-35026486

RESUMO

An in-depth understanding of the bioactive mechanism of phytochemicals has a good guiding value for the design of related functional foods. Herein, the effect of N1, N5- di-[(E)-p-coumaroyl]-spermidine (NDPS) originated from adlay on protecting HepG2 cells from oxidative stress was evaluated by MTT assay, western blot and qRT-PCR. After pre-treatment of NDPS, the activities of antioxidant enzymes (including superoxide dismutase, glutathione peroxidase, γ-glutamyl cysteine synthetase and heme oxygenase-1) were increased, as well as the level of proteins and gene expressions were elevated. Moreover, the γ-GCS, HO-1, SOD and GPx protein level were enhanced for the cells with NDPS treatment compared to both positive control and negative control groups. These findings suggested that NDPS could protect HepG2 cells from oxidative stress by increasing the antioxidant enzymes regulated by Nrf2/ARE pathway.

6.
Nat Commun ; 13(1): 246, 2022 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-35017472

RESUMO

The molecular targets and mechanisms of propolis ameliorating metabolic syndrome are not fully understood. Here, we report that Brazilian green propolis reduces fasting blood glucose levels in obese mice by disrupting the formation of CREB/CRTC2 transcriptional complex, a key regulator of hepatic gluconeogenesis. Using a mammalian two-hybrid system based on CREB-CRTC2, we identify artepillin C (APC) from propolis as an inhibitor of CREB-CRTC2 interaction. Without apparent toxicity, APC protects mice from high fat diet-induced obesity, decreases fasting glucose levels, enhances insulin sensitivity and reduces lipid levels in the serum and liver by suppressing CREB/CRTC2-mediated both gluconeogenic and SREBP transcriptions. To develop more potential drugs from APC, we designed and found a novel compound, A57 that exhibits higher inhibitory activity on CREB-CRTC2 association and better capability of improving insulin sensitivity in obese animals, as compared with APC. In this work, our results indicate that CREB/CRTC2 is a suitable target for developing anti-metabolic syndrome drugs.

7.
Toxicol Appl Pharmacol ; 436: 115855, 2022 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-34990729

RESUMO

Nonalcoholic fatty liver disease (NAFLD) is a major global public health concern affecting more than 25% of the world's population. Although obesity and diabetes are major risk factors for NAFLD, they cannot account for all cases, indicating the importance of other factors such as environmental exposures. Cadmium (Cd) exposure is implicated in the development of NAFLD; however, the influence of early life, in utero Cd exposure on the development of diet-induced NAFLD is poorly understood. Therefore, we developed an in vivo, multiple-hit model to study the effect of whole-life, low dose Cd exposure on high fat diet (HFD)-induced NAFLD. Adult male and female C57BL/6 J mice fed normal diets (ND) were exposed to 0, 0.5 or 5 ppm Cd-containing drinking water for 14 weeks before breeding. At weaning, offspring were fed ND or HFD and continued on the same drinking water regimen as their parents for 24 weeks. Cd exposure at different concentrations differentially altered HFD-associated adverse health effects, including liver injury. HFD-induced increased body weight, decreased glucose tolerance. Liver injury and lipid deposition were exacerbated by 5 ppm Cd exposure but attenuated by 0.5 ppm Cd exposure. Further, HFD blunted the response of metallothionein, a major Cd detoxification protein, in mice exposed to 5 ppm Cd but enhanced the response in mice exposed to 0.5 ppm Cd, suggesting a possible mechanism for Cd alteration of HFD-induced NAFLD. These results confirm the multi-hit nature of NAFLD and show whole life, low dose Cd exposure alters HFD-induced NAFLD with outcomes dependent on Cd concentration.

8.
Artigo em Inglês | MEDLINE | ID: mdl-34993787

RESUMO

Excessive cobalt exposure has been shown to induce various adverse health effects in animal and human toxicity studies. However, the relationships between cobalt exposure and obesity, insulin resistance, and metabolic-related disorders are rarely studied in epidemiological studies. This study aimed to explore the dose-response relationships between urinary cobalt concentrations and obesity, insulin resistance, and metabolic-related disorders. Adult participants (≥20 years) from the 2005 to 2018 National Health and Nutrition Examination Survey were included. Restricted cubic splines and the log-binomial regression were adopted. Multivariate adjusted prevalence ratios (95% confidence intervals) comparing extreme quartiles of urinary cobalt concentrations were 1.43 (1.29-1.57) for obesity, 1.43 (1.10-1.86) for insulin resistance, 1.21 (1.09-1.34) for metabolic syndrome, 1.16 (1.10-1.23) for elevated waist circumference, 1.20 (1.09-1.33) for elevated triglycerides, 1.14 (1.01-1.29) for reduced high-density lipoprotein cholesterol (HDL-C), 1.06 (0.98-1.15) for elevated blood pressure, and 0.91 (0.79-1.06) for elevated fasting glucose, respectively. Results from the restricted cubic splines showed that an initial steep increase in risk was followed by a weaker increase in risk or a platform beyond 1.0 µg/L for obesity (prevalence ratio (95% confidence interval): 1.41 (1.29-1.55)), insulin resistance (1.33 (1.07-1.65)), metabolic syndrome (1.18 (1.11-1.31)), elevated waist circumference (1.18 (1.11-1.25)), elevated triglycerides (1.21 (1.11-1.33)), and reduced HDL-C (1.15 (1.01-1.30)), respectively. In conclusion, the prevalence of obesity, insulin resistance, metabolic syndrome, elevated waist circumference, elevated triglycerides, and reduced HDL-C progressively increased with increasing urinary cobalt concentrations. The above associations are non-linear and there is not a "safe threshold" below which there are no toxic effects of cobalt.

9.
Proc Inst Mech Eng H ; 236(1): 103-120, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34617494

RESUMO

Bone cutting plays an important role in spine surgical operations. The power devices with high speed employing in bone cutting usually leads to high cutting temperature of the bone tissue. This high temperature control is important in improving cutting surface quality and optimizing the cutting parameters. In this paper, the bone-cutting model was appropriately simplified for finite element (FE) based modeling of 2D orthogonal cutting to discuss the change law of cutting temperature of cortical bones for cervical vertebra, and to study the orthogonal cutting mechanism of the anisotropic cortical bone, a 3D FE simulation model had been also established in which longitudinal, vertical, and transversal cutting types were accomplished to investigate the effect of osteons orientation. Secondly, this response surface method was used to regress the simulation results, and establishes the prediction model of maximum temperature on cutting depth, cutting speed, and feed speed. Then, the Sobol method was used to analyze the sensitivity of the milling temperature prediction mathematical model parameters, in order to clarify and quantitatively analyze the influence of input milling parameters on the output milling temperature. Finally, the cutting temperatures obtained with the simulations were compared with the corresponding experimental results obtained from the bone milling tests. This study verifies the influence of key variables and the cutting parameters on thermo mechanical behavior of the bone cutting. The obtained cutting temperature distribution for the bone surfaces could be employed to establish a theoretical foundation for research on thermal damage control of bone tissues.


Assuntos
Procedimentos Ortopédicos , Osso e Ossos/cirurgia , Osso Cortical/cirurgia , Temperatura Alta , Temperatura
10.
Talanta ; 238(Pt 1): 123005, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34857336

RESUMO

Salmonella, Escherichia coli O157:H7 (E. coli O157:H7) and Shigella flexneri (S. flexneri) might contaminate similar types of meat products and cause deadly diseases in humans. In reality, ground beef samples may carry more than one pathogen and a rapid and accurate detection method for the simultaneous identification of multiple specific pathogenic strains in ground beef is crucial. In this study, a sample pretreatment protocol and a flow cytometry method were developed for rapid and multiplexed quantification of the three pathogens without cultural enrichment in ground beef. The whole process of sample pretreatment, staining, and instrument analysis can be accomplished within 1 h. The three bacteria upon sample pretreatment were demonstrated good recoveries (93.8%-101.2%). The quantitative detection range of the mothed was 103 to 108 cells/g for all three pathogens, and the detection limit for Salmonella, E. coli O157:H7 and S. flexneri in ground beef were 3.1 × 103 cells/g, 2.1 × 103 cells/g and 2.3 × 103 cells/g, respectively. Therefore, the as-developed approach is a rapid and quantitative method for multiplexed detection of Salmonella, E. coli O157:H7, and S. flexneri in ground beef.


Assuntos
Escherichia coli O157 , Produtos da Carne , Animais , Bovinos , Contagem de Colônia Microbiana , Citometria de Fluxo , Microbiologia de Alimentos , Humanos , Salmonella , Shigella flexneri
11.
Food Funct ; 13(1): 327-343, 2022 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-34904613

RESUMO

Non-alcoholic fatty liver disease (NAFLD) has increasingly become a serious public health problem. There is growing evidence that nonylphenol (NP) exposure may cause steatosis, but the underlying mechanism is not fully understood. Curcumin (CUR) improves NAFLD-related lipid metabolism disorders and oxidative stress, but its preventive and therapeutic effects on NP-induced steatosis have not been reported. The objective of this investigation was to determine the capability and potential mechanism of NP to induce steatosis in vitro and the intervention of curcumin. HepG2 cells were treated with 0 µM, 20 µM, 30 µM, 40 µM NP for 24 h. Lipid droplets accumulated significantly in HepG2 cells after NP treatment, and the concentration of triglyceride (TG) and total cholesterol (T-CHO) increased significantly. Simultaneously, lipogenesis gene expression was up-regulated significantly, fatty acid oxidation (FAO) gene expression was significantly down-regulated, and reactive oxygen species (ROS) were overproduced. Meanwhile, the expression of p-AMPK/AMPK in the AMPK/mTOR signaling pathway was significantly down-regulated and the expression of p-mTOR/mTOR was markedly up-regulated. However, blocking ROS production with N-acetyl-L-cysteine (NAC) can reverse these phenomena. In addition, our study found that curcumin effectively ameliorated the effects of NP-induced steatosis. Our study indicates that NP can induce steatosis in HepG2 cells, and may be implicated in inhibiting the ROS-dependent AMPK/mTOR pathway, and that curcumin ameliorates the NAFLD-like changes induced by NP in HepG2 cells.

12.
J Colloid Interface Sci ; 608(Pt 1): 207-218, 2022 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-34626967

RESUMO

An efficient oxygen bifunctional catalyst Pt-Ru-Ir with ordered mesoporous nanostructures (OMNs) was successfully synthesized by chemical reduction using KIT-6 mesoporous silica as a template. The crystallographic behavior, electronic effects, and microstructure of the catalysts were investigated by XRD, XPS, SEM, and TEM analysis. The influence of OMNs and the effect of Ir content in Pt-Ru-Ir catalyst on both oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) were investigated. The synergistic and electronic effects play an important role in electrocatalytic performance through the electronic coupling between Pt, Ru and Ir followed by the alloy formation with different lattice strain percentages. Amongst, the OMNs Pt70Ru25Ir5 catalyst exhibits the highest mass activity of 0.21 mA µg-1 and specific activity of 0.33 mA cm-2 for ORR, which are nearly 5-fold greater than those for benchmark Pt/C catalyst. Furthermore, the Pt70Ru25Ir5 demonstrated enhanced OER activity with an overpotential of 470 mV at 10 mA cm-2, an onset potential of 1.70 V, and a Tafel slope of 118 mV dec-1, outperforming commercial IrO2. In addition, the durability of the Pt70Ru25Ir5 catalyst for ORR and OER are found to be extended in comparison with that of other catalysts reported in this work after 6000 cycles. These results demonstrate that the ordered OMNs Pt-Ru-Ir with low Ir content (∼5 wt%) could be a promising oxygen bifunctional catalyst for electrochemical energy conversion and storage applications.

13.
Food Chem ; 370: 131303, 2022 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-34662794

RESUMO

Pleurotus eryngii is a valuable new edible mushroom variety cultivated on a large scale in China. The polysaccharides found in this mushroom are strong bioactive. This study used simulated digestion and fermentation model to study the digestion and fermentation characteristics of Pleurotus eryngii polysaccharide (PEP) and its effect on gut microbiota. The results showed that the molecular weight of PEP remained unchanged after simulated digestion, and the overall structure of PEP was not destroyed, indicating that PEP was not decomposed during digestion. However, during fermentation, PEP was degraded and utilized by intestinal flora to produce a variety of short-chain fatty acids (SCFAs), which reduced the pH value in fecal cultures. Meanwhile, PEP regulated the composition of intestinal flora, and the relative abundance of Firmicutes increased significantly. These suggests that PEP can be used as a functional food to promote intestinal health and prevent disease.


Assuntos
Pleurotus , Digestão , Fermentação , Polissacarídeos
14.
Acta Radiol ; : 2841851211056471, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34851138

RESUMO

BACKGROUND: The pathogenesis and related factors of central nervous system abnormality in patients with type 2 diabetes mellitus (T2DM) have always been the focus of clinical research. PURPOSE: To compare and analyze the area of white matter (WM) damage in patients with T2DM based on their level of hemoglobin A1C (HBA1c) and discuss any related factors. MATERIAL AND METHODS: Based on their levels of HBA1c, 87 patients with T2DM were divided into three groups (Group B, C, or D), of which 29 non-diabetic volunteers served as the control group (Group A). DTI data analysis was based on tract-based spatial statistics (TBSS). The obtained parameters were compared among each group and the relevant clinical factors were analyzed. RESULTS: For age, sex, mini-mental state examination (MMSE), and Montreal Cognitive Assessment (MoCA) scores, there were no statistically significant differences among groups. For fractional anisotropy (FA) and radial diffusivity (RD) of WM, there were statistically significant differences (P < 0.05, two-tailed, FWE corrected) in the local area of corpus callosum, corona radiate, superior longitudinal fasciculus, etc. Most of these were significantly correlated with body mass index (BMI), left systolic blood pressure (SBP_L), and ß2 microglobulin. CONCLUSION: Before the cognitive function was obviously impaired, abnormalities of FA and RD had been found in the corpus callosum, corona radiate, and upper fasciculus in patients with T2DM, which suggested that the damage mainly occurred in the myelin sheath of WM and may be related to systemic vascular damage.

15.
Front Aging Neurosci ; 13: 749109, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34867278

RESUMO

Background: Recent years have witnessed an increasing number of studies indicating an essential role of the lysosomal dysfunction in Parkinson's disease (PD) at the genetic, biochemical, and cellular pathway levels. In this study, we investigated the association between rare variants in lysosomal storage disorder (LSD) genes and Chinese mainland PD. Methods: We explored the association between rare variants of 69 LSD genes and PD in 3,879 patients and 2,931 controls from Parkinson's Disease & Movement Disorders Multicenter Database and Collaborative Network in China (PD-MDCNC) using next-generation sequencing, which were analyzed by using the optimized sequence kernel association test. Results: We identified the significant burden of rare putative LSD gene variants in Chinese mainland patients with PD. This association was robust in familial or sporadic early-onset patients after excluding the GBA variants but not in sporadic late-onset patients. The burden analysis of variant sets in genes of LSD subgroups revealed a suggestive significant association between variant sets in genes of sphingolipidosis deficiency disorders and familial or sporadic early-onset patients. In contrast, variant sets in genes of sphingolipidoses, mucopolysaccharidoses, and post-translational modification defect disorders were suggestively associated with sporadic late-onset patients. Then, SMPD1 and other four novel genes (i.e., GUSB, CLN6, PPT1, and SCARB2) were suggestively associated with sporadic early-onset or familial patients, whereas GALNS and NAGA were suggestively associated with late-onset patients. Conclusion: Our findings supported the association between LSD genes and PD and revealed several novel risk genes in Chinese mainland patients with PD, which confirmed the importance of lysosomal mechanisms in PD pathogenesis. Moreover, we identified the genetic heterogeneity in early-onset and late-onset of patients with PD, which may provide valuable suggestions for the treatment.

16.
Front Plant Sci ; 12: 762605, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34868158

RESUMO

Fusarium head blight (FHB), a notorious plant disease caused by Fusarium graminearum (F. graminearum), is severely harmful to wheat production, resulting in a decline in grain quality and yield. In order to develop novel control strategies, metabolomics has been increasingly used to characterize more comprehensive profiles of the mechanisms of underlying plant-pathogen interactions. In this research, untargeted and targeted metabolomics were used to analyze the metabolite differences between two wheat varieties, the resistant genotype Sumai 3 and the susceptible genotype Shannong 20, after F. graminearum inoculation. The untargeted metabolomics results showed that differential amino acid metabolic pathways existed in Sumai 3 and Shannong 20 after F. graminearum infection. Additionally, some of the amino acid contents changed greatly in different cultivars when infected with F. graminearum. Exogenous application of amino acids and F. graminearum inoculation assay showed that proline (Pro) and alanine (Ala) increased wheat resistance to FHB, while cysteine (Cys) aggravated the susceptibility. This study provides an initial insight into the metabolite differences of two wheat cultivars under the stress of F. graminearum. Moreover, the method of optimization metabolite extraction presents an effective and feasible strategy to explore the understanding of the mechanisms involved in the FHB resistance.

17.
Ann Palliat Med ; 10(11): 11370-11381, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34872263

RESUMO

BACKGROUND: Dizziness is a common symptom in clinic, but there lacks an effective treatment method. This study sought to examine the efficiency of deep learning (DL)-based magnetic resonance imaging (MRI) in the diagnosis of cerebral infarction mainly manifesting as vertigo using the neutrophil to lymphocyte ratio (NLR) and other routine blood indexes. METHODS: An improved multiscale U-Net [MS (U-Net)] model, based on the U-net model, was proposed and applied in the segmentation of MRI of the brain. One hundred and fifteen vertiginous cerebral infarction (VCI) patients, admitted to the Department of Neurology at Huizhou Central People's Hospital from January 2016 to December 2020, were chosen as the research subjects. Based on the MRI segmentation results for the brain, the patients were allocated to the benign paroxysmal positional vertigo (BPPV) group or acute cerebral infarction (ACI) group. Additionally, 50 healthy individuals, whose venous blood was collected for routine blood analyses, were allocated to the control group. RESULTS: The MS (U-Net) model accomplishes MRI segmentation of the brain, and its segmentation results were much closer to the real results than those of the U-Net model. Compared to the control group, the monocyte count (MC), low-density lipoprotein/high-density lipoprotein (LDL/HDL) ratio, and NLR of patients in the BPPV and ACI groups showed an obvious increase (P<0.05), as did the white blood cell count, triglyceride (TG) level, and other indexes of ACI patients (P<0.05). In relation to the diagnosis, the areas under the curve for the TG level, LDL/HDL ratio, and NLR of the BPPV and ACI groups were 0.930 and 0.760, 0.900, and 0.770, 0.945 and 0.855, respectively (P<0.05). CONCLUSIONS: DL can accomplish MRI segmentation in cerebral infarction patients, and the TG level, LDL/HDL ratio and NLR can be used in the diagnosis of VCI.

18.
Front Physiol ; 12: 785799, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34858216

RESUMO

Background: Cardiac hypertrophy as a main pathological manifestation of diabetic cardiomyopathy (DCM), is a significant complication of diabetes. Bariatric surgery has been proven to relieve DCM; however, whether it can alleviate diabetes-induced cardiac hypertrophy is undefined. Methods: Diabetic and obese rats were performed sleeve gastrectomy (SG) after having diabetes for 16weeks. The rats were euthanized 8weeks after SG. Metabolic parameters, heart function parameters, myocardial glucose uptake, morphometric and histological changes, and the expression level of mitogen-activated protein kinases (MAPKs) were determined and compared among the control group (CON group), diabetes mellitus group (DM group), sham operation group (SHAM group), and SG group. Results: Compared with the SHAM group, the blood glucose, body weight, insulin resistance, and other metabolic parameters were significantly improved in the SG group. There was also a marked improvement in myocardial morphometric and histological parameters after SG. Furthermore, the myocardial glucose uptake and heart function were reversed after SG. Additionally, the phosphorylation of MAPKs was inhibited after SG, including p38 MAPKs, c-Jun N-terminal kinases (JNKs), and extracellular signal-regulated kinases 1/2 (ERK1/2). The expression of DUSP6, which dephosphorylates ERK1/2, was upregulated after SG. These findings suggest that SG ameliorated diabetes-induced cardiac hypertrophy correlates with the MAPK signaling pathway. Conclusion: These results showed that diabetes-induced cardiac hypertrophy was ameliorated after SG was closely related to the inhibition of the MAPK signaling pathway and upregulation of DUSP6. Therefore, this study provides a novel strategy for treating diabetes-induced cardiac hypertrophy.

19.
Inorg Chem ; 2021 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-34890197

RESUMO

Novel nonlinear optical (NLO) materials possessing simple chemical compositions and facile syntheses are competitive when considering their practical application. Here, a series of ternary selenides GaxIn2-xSe3 (x = 0.07, 0.38, 0.45, and 0.81) that crystallize in a chiral P65 structure are obtained by melting Ga, In, and Se elements. Their three-dimensional structures are built by (Ga/In)Se4 tetrahedra and InSe5 trigonal bipyramids. The hexagonal modification's phase stability is analyzed by energy calculation, and their optical band gaps are determined to be 1.72-1.99 eV. They exhibit large NLO responses that are 1.41-1.64 times that of the benchmark AgGaS2. The results of density functional theory calculations suggest that introduction of Ga onto the In site in (InSe4)5- units can form a deformed tetrahedron with more distortion in the structure, and the (InSe5)7- units contribute a large amount of birefringence to the structure. This work is the first to investigate the ternary chalcogenides M2Q3 (M = Ga or In; Q = S or Se) as new types of infrared NLO crystals with excellent performances, which will stimulate more interest in those possessing simple compositions and outstanding performances.

20.
Annu Int Conf IEEE Eng Med Biol Soc ; 2021: 6211-6214, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34892534

RESUMO

Freezing of gait (FOG) is a disabling symptom of Parkinson's disease (PD) patients, especially in advanced stages. Visual cues, such as the laser, have been confirmed to improve kinematic performance and alleviate FOG incidences. However, the muscular effect is unknown. In this study, we aim to investigate the effect of visual cues on muscular activity in the lower limbs of PD patients with FOG. Surface EMG signals of the tibialis anterior (TA), lateral gastrocnemius (GL), rectus femoris (RF), and biceps femoris (BF) muscles were collected from eight patients (FOGer) and eight healthy elderly (HC) in both normal walking and walking with laser cues. Results showed that visual cues improved FOGer's muscular activation pattern towards normal. The RMS of TA was significantly increased in the loading response phase (p=0.02) and decreased in the pre-swing phase for FOGer (p=0.005) under visual cue. The RMS of GL in FOGer was considerably reduced in the loading response phase (p<0.001) and increased in the pre-swing phase (p=0.008) of their gait cycle. A significant strong correlation was also observed between the decrement in GL RMS during the loading response phase and the increment in GL RMS during the pre-swing phase (R=-0.952, p<0.001) incurred by visual cue in FOGer. These results indicate that the visual cues can help FOGer to modulate their muscular activation of ankle muscles, especially to normalize GL's activation distribution during stance. For clinical purposes, future rehabilitative strategies aimed at the modulation of ankle muscles are suggested.

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