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1.
Chem Asian J ; 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31904185

RESUMO

Fabricating a low-cost, highly efficient electrocatalyst is of importance for the development of renewable energy devices. In this work, we have synthesized an ultrafine cobalt oxide nanocatalyst (5-10 nm) doped with boron (BC/Co 3 O 4 ) by using metal-organic framework as a precursor, which exhibits an excellent catalytic activity for oxygen evolution reaction (OER). Owing to the improvement of accessible active sites by boron doping, the synthesized catalyst can reach a current density of 10 mA cm -2 at 1.54 V with a low overpotential of 310 mV, superior than those of commercial RuO 2 and N-doped C/Co 3 O 4 . This work provides a facile way to develop highly efficient catalysts for electrochemical reactions.

2.
Plant Biotechnol J ; 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31916321

RESUMO

Fruit neck length (FNL) is an important quality trait in cucumber because it directly affects its market value. However, its genetic basis remains largely unknown. We identified a candidate gene for FNL in cucumber using a next-generation sequencing-based bulked segregant analysis in F2 populations, derived from a cross between Jin5-508 (long necked) and YN (short necked). A quantitative trait locus (QTL) on chromosome 7, Fnl7.1, was identified through a genome-wide comparison of single nucleotide polymorphisms between long- and short-FNL F2 pools, and it was confirmed by traditional QTL mapping in multiple environments. Fine genetic mapping, sequences alignment and gene expression analysis revealed that CsFnl7.1 was the most likely candidate Fnl7.1 locus, which encodes a late embryogenesis abundant protein. The increased expression of CsFnl7.1 in long-necked Jin5-508 may be attributed to mutations in the promoter region upstream of the gene body. The function of CsFnl7.1 in FNL control was confirmed by its overexpression in transgenic cucumbers. CsFnl7.1 regulate fruit neck development by modulating cell expansion. Probably, this is achieved through the direct protein-protein interactions between CsFnl7.1 and a dynamin related protein CsDRP6 and a germin-like protein CsGLP1. Geographical distribution differences of the FNL phenotype were found among the different cucumber types. The East Asian and Eurasian cucumber accessions were highly enriched with the long-necked and short-necked phenotypes, respectively. A further phylogenetic analysis revealed that the Fnl7.1 locus might have originated from India. Thus, these data support that the CsFnl7.1 has an important role in increasing cucumber FNL.

3.
Dalton Trans ; 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31894793

RESUMO

The fluorescence chemosensing behavior of Zn(ii), Cu(ii), and Cd(ii) based complexes toward cations has been described. Cation detection via conventional mechanisms, metal-metal exchange and chemodosimetric approaches along with the importance of metal ions and the scope, significance, and challenges with regard to the detection of cations by metal complex based probes will be discussed in detail. The fundamentals of photophysical behavior and mechanisms involved in the fluorescence detection of analytes will also be described. This article provides a detailed overview of Zn(ii), Cu(ii), and Cd(ii) based complexes as fluorescent probes for cations, together with essential discussions pertaining to detection mechanisms.

4.
Mol Plant Pathol ; 21(1): 83-94, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31774224

RESUMO

As an obligate parasite, Puccinia striiformis f. sp. tritici (Pst) forms haustoria to obtain nutrients from plant cells for development, and these structures are essential for pathogen survival. To better understand the contribution of haustoria to the interactions with the host plants, we isolated haustoria from susceptible wheat leaves infected with Pst race CYR31 and sequenced their transcriptome as well as those of urediospores and germ tubes, and compared the three transcriptomes. A total of 3524 up-regulated genes were obtained from haustoria, of which 73 genes were related to thiamine biosynthesis, glycolysis and lipid metabolic processes. Silencing seven of the genes reduced the growth and development of Pst in wheat. More interestingly, 1197 haustorial secreted proteins (HASPs) were detected in haustoria, accounting for 34% of the total proteins, indicating that these HASPs play important roles in haustorium-mediated pathogenic progression. Furthermore, 69 HASPs were able to suppress Bax-triggered programmed cell death in tobacco. Additionally, 46 HASPs significantly reduced callose deposition in wheat using the type III secretion system. This study identified a large number of effectors through transcriptome sequencing, and the results revealed components of metabolic pathways that impact the growth and colonization of the pathogen and indicate essential functions of haustoria in the growth and pathogenicity of Pst.

5.
Chem Soc Rev ; 2019 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-31832631

RESUMO

As a new class of crystalline porous materials, metal-organic frameworks (MOFs) have received great attention owing to their unique advantages of ultrahigh surface area, large pore volume and versatile applications. Developing different strategies to control the morphology and size of MOFs is very important for their practical applications. Recently, micro/nanosized MOFs have been regarded as promising candidates for electrode materials with excellent performances, which not only bridge the gap between fundamental MOF science and forward-looking applications, but also provide an opportunity to make clear the relationship between morphologies and properties. This review focuses on the design and fabrication of one-, two- and three-dimensional MOFs at micro/nanoscale, and their direct applications in batteries, supercapacitors and electrocatalysis. A discussion on challenges and future prospects of the synthesis and electrochemical applications of micro/nanoscaled MOF materials is presented.

6.
Pharmacol Res ; : 104595, 2019 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-31838080

RESUMO

Src homology 2 (SH2)-containing protein tyrosine phosphatase 2 (SHP2) is a major phosphatase involved in several cellular processes. In recent years, SHP2 has been the focus of significant attention in human diseases, particular in cancer. Several studies have shown that SHP2 plays an important role in regulating immune cell functions in tumor microenvironment. A few clinical trials conducted using SHP2 allosteric inhibitors have shown remarkable anti-tumor benefits and good safety profiles. This review focuses on the current understanding of the regulation of SHP2 and highlights the vital roles of SHP2 in T lymphocytes, macrophages and cancer cells. It also summarizes the current development of SHP2 inhibitors as a promising strategy for cancer immunotherapy.

7.
ISA Trans ; 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31818488

RESUMO

It is well known that hydraulic systems typically suffer from heavy disturbances including parametric uncertainties and unknown disturbances. In order to attain high performance tracking control, this paper proposes a composite design of nonlinear neural-networks (NN) and continuous robust integral of the sign of the error (RISE) feedback controller. The control development incorporates a NN feedforward component to have a compensation for unknown state-dependent disturbances and to further improve the accuracy of feedforward compensation, meanwhile input parameter is updated online. To achieve asymptotic stability, a novel RISE term with NN-based feedforward component is developed for the first time to enable the incorporation of model reference adaptive control structure where acceleration signal is not employed. The proposed controller guarantees controlled hydraulic system a semi-global asymptotic stability. For the experimental results, the prescribed transient performance is tested under rectangular trajectory and the steady state performance is tested under sinusoidal trajectory.

8.
Mol Psychiatry ; 2019 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-31827248

RESUMO

The Chinese Imaging Genetics (CHIMGEN) study establishes the largest Chinese neuroimaging genetics cohort and aims to identify genetic and environmental factors and their interactions that are associated with neuroimaging and behavioral phenotypes. This study prospectively collected genomic, neuroimaging, environmental, and behavioral data from more than 7000 healthy Chinese Han participants aged 18-30 years. As a pioneer of large-sample neuroimaging genetics cohorts of non-Caucasian populations, this cohort can provide new insights into ethnic differences in genetic-neuroimaging associations by being compared with Caucasian cohorts. In addition to micro-environmental measurements, this study also collects hundreds of quantitative macro-environmental measurements from remote sensing and national survey databases based on the locations of each participant from birth to present, which will facilitate discoveries of new environmental factors associated with neuroimaging phenotypes. With lifespan environmental measurements, this study can also provide insights on the macro-environmental exposures that affect the human brain as well as their timing and mechanisms of action.

9.
Front Plant Sci ; 10: 1407, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31787996

RESUMO

A highly reproducible plant electrical signal-light-induced bioelectrogenesis (LIB) was obtained by means of periodic illumination/darkness stimulation of broad bean (Vicia faba L.) leaves. By stimulating the same position of the same leaf with different concentrations of NaCl, we observed that the amplitude and waveform of the LIB was correlated with the intensity of stimulation. This method allowed us to link dynamic ion fluxes induced by periodic illumination/darkness to salt stress. The self-referencing ion electrode technique was used to explore the ionic mechanisms of the LIB. Fluxes of H+, Ca2+, K+, and Cl- showed periodic changes under periodic illumination/darkness before and after 50 mM NaCl stimulation. Gray relational analysis was used to analyze correlations between each of these ions and LIB. The results showed that different ions are involved in surface potential changes at different stages under periodic illumination/darkness. The gray relational grade reflected the contribution of each ion to the change in surface potential at a certain time period. The ion fluxes data obtained under periodic illumination/darkness stimulation will contribute to the future development of a dynamic model for interpretation of electrophysiological events in plant cells.

10.
Front Neurol ; 10: 1201, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31798523

RESUMO

Objectives: To investigate the performance of substate classification of children with benign epilepsy with centrotemporal spikes (BECTS) by granger causality density (GCD) based support vector machine (SVM) model. Methods: Forty-two children with BECTS (21 females, 21 males; mean age, 8.6 ± 1.96 years) were classified into interictal epileptic discharges (IEDs; 11 females, 10 males) and non-IEDs (10 females, 11 males) substates depending on presence of central-temporal spikes or not. GCD was calculated on four metrics, including inflow, outflow, total-flow (inflow + outflow) and int-flow (inflow - outflow) connectivity. SVM classifier was applied to discriminate the two substates. Results: The Rolandic area, caudate, dorsal attention network, visual cortex, language networks, and cerebellum had discriminative effect on distinguishing the two substates. Relative to each of the four GCD metrics, using combined metrics could reach up the classification performance (best value; AUC, 0.928; accuracy rate, 90.83%; sensitivity, 90%; specificity, 95%), especially for the combinations with more than three GCD metrics. Specially, combined the inflow, outflow and int-flow metric received the best classification performance with the highest AUC value, classification accuracy and specificity. Furthermore, the GCD-SVM model received good and stable classification performance across 14 dimension reduced data sets. Conclusions: The GCD-SVM model could be used for BECTS substate classification, which might have the potential to provide a promising model for IEDs detection. This may help assist clinicians for administer drugs and prognosis evaluation.

11.
Nat Commun ; 10(1): 5571, 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31804478

RESUMO

Chloroplasts are important for photosynthesis and for plant immunity against microbial pathogens. Here we identify a haustorium-specific protein (Pst_12806) from the wheat stripe rust fungus, Puccinia striiformis f. sp. tritici (Pst), that is translocated into chloroplasts and affects chloroplast function. Transient expression of Pst_12806 inhibits BAX-induced cell death in tobacco plants and reduces Pseudomonas-induced hypersensitive response in wheat. It suppresses plant basal immunity by reducing callose deposition and the expression of defense-related genes. Pst_12806 is upregulated during infection, and its knockdown (by host-induced gene silencing) reduces Pst growth and development, likely due to increased ROS accumulation. Pst_12806 interacts with the C-terminal Rieske domain of the wheat TaISP protein (a putative component of the cytochrome b6-f complex). Expression of Pst_12806 in plants reduces electron transport rate, photosynthesis, and production of chloroplast-derived ROS. Silencing TaISP by virus-induced gene silencing in a susceptible wheat cultivar reduces fungal growth and uredinium development, suggesting an increase in resistance against Pst infection.

12.
PLoS Biol ; 17(12): e3000525, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31841517

RESUMO

Ubiquitin-specific protease (USP) 6 is a hominoid deubiquitinating enzyme previously implicated in intellectual disability and autism spectrum disorder. Although these findings link USP6 to higher brain function, potential roles for USP6 in cognition have not been investigated. Here, we report that USP6 is highly expressed in induced human neurons and that neuron-specific expression of USP6 enhances learning and memory in a transgenic mouse model. Similarly, USP6 expression regulates N-methyl-D-aspartate-type glutamate receptor (NMDAR)-dependent long-term potentiation and long-term depression in USP6 transgenic mouse hippocampi. Proteomic characterization of transgenic USP6 mouse cortex reveals attenuated NMDAR ubiquitination, with concomitant elevation in NMDAR expression, stability, and cell surface distribution with USP6 overexpression. USP6 positively modulates GluN1 expression in transfected cells, and USP6 down-regulation impedes focal GluN1 distribution at postsynaptic densities and impairs synaptic function in neurons derived from human embryonic stem cells. Together, these results indicate that USP6 enhances NMDAR stability to promote synaptic function and cognition.

13.
Cell Death Dis ; 10(12): 957, 2019 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-31862870

RESUMO

Radiation-induced lung injury (RILI) is one of the most common and fatal complications of thoracic radiotherapy, whereas no effective interventions are available. Andrographolide, an active component extracted from Andrographis paniculate, is prescribed as a treatment for upper respiratory tract infection. Here we report the potential radioprotective effect and mechanism of Andrographolide on RILI. C57BL/6 mice were exposed to 18 Gy of whole thorax irradiation, followed by intraperitoneal injection of Andrographolide every other day for 4 weeks. Andrographolide significantly ameliorated radiation-induced lung tissue damage, inflammatory cell infiltration, and pro-inflammatory cytokine release in the early phase and progressive fibrosis in the late phase. Moreover, Andrographolide markedly hampered radiation-induced activation of the AIM2 inflammasome and pyroptosis in vivo. Furthermore, bone marrow-derived macrophages (BMDMs) were exposed to 8 Gy of X-ray radiation in vitro and Andrographolide significantly inhibited AIM2 inflammasome mediated-pyroptosis in BMDMs. Mechanistically, Andrographolide effectively prevented AIM2 from translocating into the nucleus to sense DNA damage induced by radiation or chemotherapeutic agents in BMDMs. Taken together, Andrographolide ameliorates RILI by suppressing AIM2 inflammasome mediated-pyroptosis in macrophage, identifying Andrographolide as a novel potential protective agent for RILI.

14.
Med Sci Monit ; 25: 9702-9711, 2019 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-31851643

RESUMO

BACKGROUND This study was to investigate the correlation between osteoporosis and serum uric acid in ankylosing spondylitis (AS) patients, and to further identify potential factors that might be associated with osteoporosis in AS patients. MATERIAL AND METHODS We included 182 AS patients, consisted of 143 male patients and 39 female patients, who visited our hospital from January 1, 2014 to December 31, 2018. We used dual-energy x-ray absorptiometry to measure bone mineral density (BMD) of orthotopic lumbar vertebrae in patients with AS. The gender, age, disease duration, BMD, T-score, Z-score, uric acid, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), blood platelet (PLT), and status of treatment with biologics of the patients were collected. Then, the Spearman correlation coefficient and multivariate liner regression analysis were applied to identify the relationship between the factors and BMD, T-score, and Z-score in AS patients. RESULTS Male AS patients between the ages of 16 and 30 years old had a higher risk of osteoporosis (P<0.05). AS patients with uric acid value between 300-360 µmol/L had the highest BMD, T-score, and Z-score. The BMD had a positive correlation with age and disease duration (P<0.01) while had a negative correlation with PLT (P<0.05). BMD in AS patients with elevated ESR was significantly (P<0.05) lower than in AS patients with normal ESR. There were no significant differences in BMD between AS patients with elevated CRP and the patients with normal CRP and PLT. Treatment with TNFi (tumor necrosis factor alpha inhibitor) did not improve BMD in AS patients. CONCLUSIONS The relationship between uric acid and BMD in AS patients was observed as inverted "U"-type. Keeping uric acid within 300-360 µmol/L might be helpful in preventing AS patients from developing osteoporosis.

15.
Chemosphere ; 245: 125547, 2019 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-31864950

RESUMO

Pot-culture experiments were conducted to investigate the potential of microorganism-saponin assisted phytoremediation of cadmium (Cd) and benzo(a)pyrene (B[a]P) co-contaminated soil using Cd-hyperaccumulator Sedum alfredii. Results showed that B[a]P-degrading bacterium (Ochrobactrum intermedium B[a]P-16) inoculation significantly increased root (by 22.1-24.1%) and shoot (by 20.5-23.4%) biomass of S. alfredii, whereas the application of saponin had no effect on the growth of S. alfredii. The saponin solution at 2 g L-1 extracted more Cd and B[a]P than water, saponin enhanced Cd and B[a]P bioavailability in soil and thus promoted their uptake and accumulation in S. alfredii. The activity of B[a]P-16, dehydrogenase and polyphenol oxidase in co-contaminated soil was promoted by growing S. alfredii, and the application of B[a]P-16 and saponins caused a significant (P < 0.05) increase in both enzyme activities. The maximum B[a]P removal rate (82.0%) and Cd phytoextraction rate (19.5%) were obtained by co-application of S. alfredii with B[a]P-16 and saponin. The B[a]P-16 and plant promoted biodegradation were the predominant contributors towards removal of B[a]P from soil. A significant (P < 0.05) synergistic effect of B[a]P-16 and saponin on B[a]P and Cd removal efficiency was observed in this study. It is suggested that planting S. alfredii with application of B[a]P-16 and saponin would be an effective method for phytoremediation of soil co-contaminated with Cd and PAHs.

16.
Sci Total Environ ; 707: 136049, 2019 Dec 11.
Artigo em Inglês | MEDLINE | ID: mdl-31874396

RESUMO

Soil aggregate stability and soil organic carbon (SOC) physical sequestration is essential to regulation of anthropogenic climate change. However, relative knowledge remains elusive. The total SOC stock, aggregate stability, capacity of physically protected C, structure of macroaggregates and Al/Fe oxides under rice-wheat rotation (RW), rice-vegetable rotation (RV) and afforested land (AL) were analysed. We chose 1-2 mm macroaggregates for low-temperature ashing (LTA) treatment to mimic natural oxidation to assess the capacity of physically protected C. Using scanning electron microscopy, the N adsorption method, and energy dispersive spectroscopy, we explored the internal structure of macroaggregates under different land use types. All land use types could physically protect over 50% of SOC. AL showed the strongest capacity of C sequestration, followed by RW, which preserved 67.1% and 59.6% of SOC, respectively. After 5 h of LTA treatment, the amount of SOC removed from the macropores in cropland (RW and RV) was higher than that in AL. In micropores with further oxidation, AL and RW both lost only 5% of SOC. Fe oxides were more correlated with C dynamics than Al oxides. Free Fe oxides were associated with the easily oxidised organic matter. Soil aggregate stability significantly correlated with Al/Fe oxides (p < 0.05). The RW and AL had a greater soil aggregate stability than the RV owing to the relatively higher content of Al/Fe oxides. In conclusion, the conversion of RW to RV reduced the mechanical stability of soil aggregates and the capacity of C physical sequestration, while the conversion of RW to AL increased these two properties. Land use change affected C physical sequestration mainly via changes in surface area, pore development and the content of Fe oxides in macroaggregates.

17.
Zhongguo Zhong Yao Za Zhi ; 44(22): 4786-4792, 2019 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-31872583

RESUMO

The consistency of drug quality is related to the clinical efficacy and safety,which is highly valued by the government and relevant industries. Compared with chemical medicine,traditional Chinese medicine originates from the nature,and is greatly influenced by natural factors,such as the place of origin,cultivation and processing technology,climate. The quality consistency of traditional Chinese medicine is poor. The quality consistency has become the pain point and difficulty of the development of the traditional Chinese medicine industry,which seriously affects the stability and controllability of clinical efficacy and the reproducibility or recognition of modern research results. It is also a bottleneck for Chinese patent medicine to enter the international market. Mixed batch blending technology is an effective method for the scientific guarantee of the quality consistency in other industries and disciplines,and widely applied in liquor making industry,tobacco industry and perfume industry. Overseas,mixed batch blending technology has been successfully applied in guaranteeing the quality consistency of Ginkgo biloba preparations Jinnado. It has been used in the production of Chinese formulations in Japan for more than 30 years. In recent years,mixed batch blending technology has been introduced into the pharmaceutical field to ensure the quality consistency of traditional Chinese medicine,and relevant research has gradually increased.This manuscript reviews the application of blending technology in other disciplines,summarizes the algorithm principles and software systems of mixed batch blending in traditional Chinese medicine or natural medicine,explains the specific implementation process of mixed batch blending technology,and looks forward to the application prospects of artificial intelligence and other new technologies,in the hope of providing new ideas and technologies for breaking through the problem of quality consistency,and boosting the high-quality and high-level development of Chinese medicine industry in the new era.


Assuntos
Medicamentos de Ervas Chinesas , Medicina Tradicional Chinesa , Algoritmos , Indústria Farmacêutica , Reprodutibilidade dos Testes , Tecnologia
18.
J Colloid Interface Sci ; 563: 328-335, 2019 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-31887696

RESUMO

Two dimensional (2D) ultrathin nonprecious metal based catalysts show excellent electrocatalytic activities, due to the larger surface areas, more catalytic sites and more interconnected electron-transfer access than their bulk counterparts. Here, we synthesized cobalt pyrophosphate (Co2P2O7) nanosheets with different thickness by a simple and efficient one-step hydrothermal process. The catalytic performance of the obtained Co2P2O7 was investigated via diverse electrochemical measurement. Due to the unique 2D structure and the flexible coordination of pyrophosphate group, the as-prepared Co2P2O7 catalyst had excellent electrocatalytic performance and good stability, which could rank among the most active nonprecious metal catalysts for oxygen evolution reaction and oxygen reduction reaction. In addition, the ultrathin Co2P2O7 nanosheets exhibited good performance as the air cathode catalyst for zinc air batteries.

19.
BMC Pharmacol Toxicol ; 20(1): 86, 2019 12 21.
Artigo em Inglês | MEDLINE | ID: mdl-31864413

RESUMO

BACKGROUND: T lymphocytes play an important role in contact hypersensitivity. This study aims to explore the immunosuppressive activity of SBF-1, an analog of saponin OSW-1, against T lymphocytes in vitro and in vivo. METHODS: Proliferation of T lymphocytes from lymph nodes of mice was determined by MTT assay. Flow cytometry analysis was performed to assess T cell activation and apoptosis. Levels of cytokines were determined by PCR and ELISA. BALB/c mice were sensitized and challenged with picryl chloride and thickness of left and right ears were measured. RESULTS: SBF-1 effectively inhibited T lymphocytes proliferation induced by concanavalin A (Con A) or anti-CD3 plus anti-CD28 at a very low dose (10 nM) but exhibited little toxicity in non-activated T lymphocytes at concentrations up to 10 µM. In addition, SBF-1 inhibited the expression of CD25 and CD69, as well as he phosphorylation of AKT in Con A-activated T cells. SBF-1 also induced apoptosis of activated T cells. In addition, SBF-1 also downregulated the induction of the T cell cytokines, IL-2 and IFN-γ in a dose-dependent manner. Furthermore, SBF-1 significantly suppressed ear swelling and inflammation in a mouse model of picryl chloride-induced contact hypersensitivity. CONCLUSIONS: Our findings suggest that SBF-1 has an unique immunosuppressive activity both in vitro and in vivo mainly through inhibiting T cell proliferation and activation. Its mechanism appears to be related to the blockage of AKT signaling pathway.

20.
Artigo em Inglês | MEDLINE | ID: mdl-31849079

RESUMO

BACKGROUND: It remains unclear whether the curative result of paroxysmal atrial fibrillation (PAF) is a result of pulmonary vein (PV) isolation, PV antrum modification, or both. We hypothesized that sufficient antrum modification (PVAM) is as important as PV isolation for AF control and that PVAM can be evaluated by quantified lesion deployment using a force-sensing catheter. METHODS AND RESULTS: Patients of symptomatic PAF were randomly assigned 2:1 into a PVAM group or a circumferential pulmonary vein isolation (CPVI) group. In the PVAM group, circumferential quantitative ablation evaluated by automatical VisiTag module was performed. In the CPVI group, conventional circumferential ablation was performed to achieve the end point of all-PV isolation. In total, 180 patients with PAF were enrolled and randomly assigned to either the PVAM group (n = 120) or the CPVI group (n = 60). A total of 179 patients successfully underwent ablation. In the PVAM group, 68 patients achieved all PV isolation (PVAM-PVI), while 51 did not (PVAM-non-PVI). At 18 months, there was no significant difference in the maintenance of sinus rhythm between the PVAM and CPVI groups (84.9% vs. 79.7%, p = 0.382). The PVAM-PVI subgroup demonstrated a higher arrhythmia-free survival compared with the PVAM-non-PVI subgroup (92.6% vs. 74.5%, p = 0.006) and the CPVI group (92.6% vs. 79.7%, p = 0.036). CONCLUSIONS: The trial shows that sufficient force-sensing guided PVAM can result in satisfying outcomes in PAF patients. Notably, sufficient PVAM with all-PV isolated will further increase the success rate. This article is protected by copyright. All rights reserved.

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