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1.
Int Immunopharmacol ; 101(Pt B): 108316, 2021 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-34768129

RESUMO

PURPOSE: Previously, we reported the octyl ester derivative of ginsenoside Rh2 (Rh2-O) had better antitumor and immunomodulatory effects than Rh2 in H22 tumor-bearing mice. Therefore, this study further explored the effects of Rh2-O on splenic lymphocytes in H22 tumor-bearing mice and the underlying mechanism. METHODS: Wild type and Tlr4-/- mice were selected to establish the H22 tumor-bearing mice model. After the treatment of Rh2-O (10 mg/kg by gavage) for 15 days, the sizes of tumor were measured. Subsequently, the splenic lymphocytes were isolated and the activities (eg. cell proliferation, cytotoxicity and cytokine secretion) were evaluated. Then, the proteins and mRNA expression levels of TRAF6 and NF-ĸB p65 in splenic lymphocytes were examined. RESULTS: The results showed that Rh2-O administration enhanced the proliferative capacity and cytotoxicity of splenic lymphocytes, and the effects were Tlr4-associated. Compared to WT mice, the up-regulation of cytokines secretion (eg. IFN-γ, IL-2 and IL-4) in isolated splenic lymphocytes after Rh2-O administration was lower in Tlr4-/- mice. Moreover, the results showed Rh2-O increased the expression of TRAF6 and the level of endonuclear NF-ĸB p65, which was inhibited in Tlr4-/- mice (P < 0.05). CONCLUSION: Rh2-O could exert immunomodulatory effects on splenic lymphocytes with the partial participation of TLR4 in H22 tumor-bearing mice.

2.
RSC Med Chem ; 12(10): 1771-1779, 2021 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-34778778

RESUMO

In anti-tumour therapy, the toll-like receptor 2/4 (TLR2/4) signalling pathway has been a double-edged sword. TLR2/4 agonists are commonly considered adjuvants for immune stimulation, whereas TLR2/4 antagonists demonstrate more feasibility for anti-tumour therapy under specific chronic inflammatory situations. In individuals with cancer retaliatory proliferation and metastasis after surgery, blocking the TLR2/4 signalling pathway may produce favourable prognosis for patients. Therefore, here, we developed a small-molecule co-inhibitor that targets the TLR2/4 signalling pathway. After high-throughput screening of a compound library containing 14 400 small molecules, followed by hit-to-lead structural optimisation, we finally obtained the compound TX-33, which has effective inhibitory properties against the TLR2/4 signalling pathways. This compound was found to significantly inhibit multiple pro-inflammatory cytokines released by RAW264.7 cells. This was followed by TX-33 demonstrating promising efficacy in subsequent anti-tumour experiments. The current results provide a novel understanding of the role of TLR2/4 in cancer and a novel strategy for anti-tumour therapy.

3.
Environ Res ; : 112321, 2021 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-34748777

RESUMO

BACKGROUND: Pulmonary embolism (PE) is a life-threatening condition. Few studies have evaluated the relationship between air pollution and PE, and these results have been inconsistent. Therefore, our study aimed to investigate the association between air pollutant exposure and the risk of hospitalization due to PE. MATERIALS AND METHODS: Daily PE admissions, meteorological data, and ambient pollution data from January 1, 2015, to December 31, 2018, were collected in Beijing. A quasi-Poisson regression model combined with time-stratified case-crossover design and a distributed lag nonlinear model was used to determine the effect of air pollutant exposure on PE admission. To examine the stability of air pollutants' effects, multi-pollutant analyses were performed. Stratified analyses by age and sex were further conducted. RESULTS: There were 5060 PE admissions during the study period, with an estimated incidence of 6.5 per 100,000. PM2.5, PM10, SO2, O3 and CO exposures were significantly associated with elevated risk of PE hospitalization. The highest cumulative risks were observed at a lag of 0-28 days for PM2.5 (relative risk [RR] = 1.056, 95% confidence intervals [CI]: 1.015-1.098), PM10 (RR = 1.042, 95%CI: 1.010-1.075), and CO (RR = 1.466, 95%CI: 1.127-1.906), at a lag of 0-27 days for SO2 (RR = 1.674, 95%CI: 1.200-2.335), and at a lag of 0-4 days for O3 (RR = 1.019, 95%CI: 1.001-1.038). All associations mentioned above except O3 remained significant in multi-pollutant models. Stratified analyses showed that women and those aged ≥65 years people were more sensitive to PM10 and CO exposure than men and those aged <65 years. The effect of PM2.5 exposure was statistically significant in all subgroups. CONCLUSIONS: Exposure to PM2.5, PM10, SO2, and CO showed a positive association with PE hospitalization. High-risk PE groups should take special precautions on days with poor air quality.

4.
Chem Commun (Camb) ; 2021 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-34812824

RESUMO

Two-dimensional bi-metallic NiFe nitrides (2D NiFe-N) are successfully synthesized in the designed ternary deep eutectic solvents under the guidance of DFT calculations. Taking advantage of the unique properties of large-size, ultrathin amorphous 2D structure and modulable electronic structure, the NiFe0.05-N exhibits extraordinary OER performance with relatively low overpotential of 238 mV at 10 mA cm-2 and durable stability.

5.
Adv Sci (Weinh) ; : e2102493, 2021 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-34668340

RESUMO

Since the discovery of 2D materials, 2D material nanofiltration (NF) membranes have attracted great attention and are being developed with a tremendously fast pace, due to their energy efficiency and cost effectiveness for water purification. The most attractive aspect for 2D material NF membranes is that, anomalous water and ion permeation phenomena have been constantly observed because of the presence of the severely confined nanocapillaries (<2 nm) in the membrane, leading to its great potential in achieving superior overall performance, e.g., high water flux, high rejection rates of ions, and high resistance to swelling. Hence, fundamental understandings of such water and ion transport behaviors are of great significance for the continuous development of 2D material NF membranes. In this work, the microscopic understandings developed up to date on 2D material NF membranes regarding the abnormal transport phenomena are reviewed, including ultrafast water and ion permeation rates with the magnitude several orders higher than that predicted by conventional diffusion behavior, ion dehydration, ionic Coulomb blockade, ion-ion correlations, etc. The state-of-the-art structural designs for 2D material NF membranes are also reviewed. Discussion and future perspectives are provided highlighting the rational design of 2D material membrane structures in the future.

6.
Chem Commun (Camb) ; 57(72): 9072-9075, 2021 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-34498618

RESUMO

Two-dimensional (2D) nonlayered ferromagnets displaying high Curie temperatures, sizable magnetic anisotropy levels, and large spin polarizations are emerging as promising 2D ferromagnetics. However, the difficulties in synthesizing 2D nonlayered intrinsic ferromagnets have largely limited their development. Herein, defect-rich 2D nonlayered VO2(M) nanosheets have been fabricated by deploying straining engineering of CO2 on the metal-insulator transition (MIT) of VO2. Above TMIT, the strong strain engineering of CO2 in the R phase of VO2 generated a very large number of atomic defects in its 3D crystal structure, and as a result facilitated conversion of the defective 3D network to 2D nanosheets along the c-axis. The as-prepared 2D defective VO2(M) nanosheets displayed unique room-temperature ferromagnetism, attributed to the symmetry breaking triggered by the disordered atomic structure combined with the 3D-to-2D transformation.

7.
Cancer Manag Res ; 13: 6823-6832, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34512022

RESUMO

Objective: Investigate the CEUS enhancement patterns of PDAC and analyse correlations between the CEUS enhancement pattern and both the degree of tumour tissue differentiation and overall survival (OS). Methods: The study included 56 patients with locally advanced PDAC, performed conventional ultrasound and CEUS, and analysed characteristics of the CEUS enhancement patterns. In addition, clinical data, such as serum level of CA19-9, TNM stage were collected, and patients' survival times were followed up. TICs of dynamic CEUS images were acquired using image processing software to obtain the peak, TP, sharpness, and AUC. Correlations of the CEUS enhancement patterns of PDAC with the degree of differentiation of tumour tissue and OS were quantitatively analysed, as were the correlations of the TIC parameters and CEUS enhancement patterns with OS. Results: Enhancement in the arterial phase included iso-enhancement (30.3%) and hypo-enhancement (69.6%), and was not significantly correlated with sex, age at disease onset, or lesion size. Also was not significantly correlated with tumour tissue differentiation. Clear survival times were obtained for 50 patients during follow-up, and the median survival time was significantly longer for the patients with iso-enhancement than hypo-enhancement. Among the TIC parameters, peaktumour, sharpnesstumour, AEsharpness, and REsharpness differed significantly between the group with iso-enhancement and hypo-enhancement (p < 0.05). Conclusion: The CEUS enhancement patterns of PDAC in the arterial phase include iso-enhancement and hypo-enhancement. Enhancement pattern was not significantly correlated with the degree of differentiation of tumour tissue, but patient survival time differed significantly between the two enhancement patterns, with longer survival for patients with iso-enhancement.

8.
Environ Pollut ; 289: 117914, 2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34426185

RESUMO

Chronic arsenic exposure can induce cardiovascular diseases through oxidative stress injury, inflammation and endothelial dysfunction. However, evidence for hypertension related to chronic arsenic exposure remains limited and inconclusive. To investigate the association of arsenic exposure with hypertension, we reviewed cross-sectional, case-control and longitudinal studies among general population (only excluded pregnant woman and children). Compared with non-exposure (or low exposure) group, we assessed relationship between the exposure (or high exposure) of arsenic and hypertension or blood pressure. We also examined the dose-response relationship to provide a theoretical basis for precision interventions in the population by conducting a two-stage random-effects dose-response meta-analysis. The weighted mean difference (WMD) was calculated to compare the blood pressure of exposed and reference arsenic exposure groups. Twenty-seven studies comprising 117,769 participants were included. The pooled OR and RR for hypertension compared the highest with lowest arsenic exposure categories were 1.14 [95 % confidence interval (CI): 1.06-1.23] and 1.30 [95 % CI: 0.91-1.84], respectively. A dose-response assessment of six studies with the necessary data available demonstrated that the OR of hypertension increased with an increasement of arsenic exposure. The differences in systolic blood pressure levels between the exposed and reference groups were 4.03 mmHg (95 % CI: 1.24-6.82). The meta-analysis further revealed the significant association of chronic arsenic exposure with hypertension among cross-sectional studies and the marginal significance in cohort studies. Besides, arsenic was mainly associated with increasing systolic blood pressure but not significantly related to diastolic blood pressure. We also demonstrated statistical evidence of a nonlinear dose-response association. Details on mechanisms and cohort studies are required to elucidate population-wide health influence of chronic arsenic exposure.


Assuntos
Arsênio , Doenças Cardiovasculares , Hipertensão , Arsênio/toxicidade , Pressão Sanguínea , Estudos Transversais , Feminino , Humanos , Hipertensão/induzido quimicamente , Hipertensão/epidemiologia
9.
Front Aging Neurosci ; 13: 687001, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34426730

RESUMO

Widespread impairments in white matter and cerebrovascular integrity have been consistently implicated in the pathophysiology of patients with small vessel disease (SVD). However, the neural circuit mechanisms that underlie the developing progress of clinical cognitive symptoms remain largely elusive. Here, we conducted cross-modal MRI scanning including diffusion tensor imaging and arterial spin labeling in a cohort of 113 patients with SVD, which included 74 patients with vascular mild cognitive impairment (vMCI) and 39 patients without vMCI symptoms, and hence developed multimode imaging-based machine learning models to identify markers that discriminated SVD subtypes. Diffusion and perfusion features, respectively, extracted from individual white matter and gray matter regions were used to train three sets of classifiers in a nested 10-fold fashion: diffusion-based, perfusion-based, and combined diffusion-perfusion-based classifiers. We found that the diffusion-perfusion combined classifier achieved the highest accuracy of 72.57% with leave-one-out cross-validation, with the diffusion features largely spanning the capsular lateral pathway of the cholinergic tracts, and the perfusion features mainly distributed in the frontal-subcortical-limbic areas. Furthermore, diffusion-based features within vMCI group were associated with performance on executive function tests. We demonstrated the superior accuracy of using diffusion-perfusion combined multimode imaging features for classifying vMCI subtype out of a cohort of patients with SVD. Disruption of white matter integrity might play a critical role in the progression of cognitive impairment in patients with SVD, while malregulation of coritcal perfusion needs further study.

10.
Environ Pollut ; 285: 117670, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34380231

RESUMO

Severe surface ozone pollution has become widespread in China. To protect public health, Chinese scientific communities and government agencies have striven to mitigate ozone pollution. However, makers of pollution mitigation policies rarely consider epidemiological research, and communication between epidemiological researchers and the government is poor. Therefore, this article reviews the current mitigation policies and the National Ambient Air Quality Standard (NAAQS) for ozone from an epidemiological perspective and proposes recommendations for researchers and policy makers on the basis of epidemiological evidence. We review current nationwide ozone control measures for mitigating ozone pollution from four dimensions: the integration of ozone and particulate matter control, ozone precursors control, ozone control in different seasons, and regional cooperation on the prevention of ozone pollution. In addition, we present environmental and epidemiological evidence and propose recommendations and discuss relevant ozone metrics and the criteria values of the NAAQS. We finally conclude that the disease burden attributable to ozone exposure in China may be underestimated and that the epidemiological research regarding the health effects of integrating ozone and particulate matter control is insufficient. Furthermore, atmospheric volatile organic compounds are severely detrimental to health, and related control policies are urgently required in China. We recommend a greater focus on winter ozone pollution and conclude that the health benefits of regional cooperation on ozone control and prevention are salient. We argue that daily average ozone concentration may be a more biologically relevant ozone metric than those currently used by the NAAQS, and accumulating epidemiological evidence supports revision of the standards. This review provides new insight for ozone mitigation policies and related epidemiological studies in China.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Ozônio , Poluentes Atmosféricos/análise , Poluição do Ar/análise , Poluição do Ar/prevenção & controle , China , Monitoramento Ambiental , Humanos , Ozônio/análise , Material Particulado/análise
11.
Planta Med ; 2021 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-34388833

RESUMO

When testing botanical ingredients of herbal medicines and dietary supplements, the complexity of botanical matrixes often requires the use of orthogonal methods to establish identification procedures suitable for quality control purposes. Genomic-based botanical identification methods are evolving and emerging as useful quality control tools to complement traditional morphological and chemical identification methods. Species-specific polymerase chain reaction methods are being evaluated for botanical quality control and as a cost-effective approach to identify and discriminate between closely related botanical species. This paper describes orthogonal identification of Panax ginseng, P. quinquefolius, and P. notoginseng materials in commerce as an example of the development and validation of a set of species-specific polymerase chain reaction methods to establish botanical identity in ginseng roots. This work also explored the possibility of extending the application of species-specific polymerase chain reaction methods to provide species identity information for processed materials, such as steamed roots and hydroalcoholic extracts, and showed success with this approach. Finally, the paper provides recommendations for an out-of-specification investigation of samples that may pass some of the orthogonal tests and fail others.

12.
Neuroimage ; 240: 118381, 2021 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-34252528

RESUMO

Diffusion weighted imaging (DWI) is a widely recognized neuroimaging technique to evaluate the microstructure of brain white matter. The objective of this study is to establish an improved automated DWI marker for estimating white matter integrity and investigating ageing related cognitive decline. The concept of Wasserstein distance was introduced to help establish a new measure: difference in distribution functions (DDF), which captures the difference of reshaping one's mean diffusivity (MD) distribution to a reference MD distribution. This new DWI measure was developed using a population-based cohort (n=19,369) from the UK Biobank. Validation was conducted using the data drawn from two independent cohorts: the Sydney Memory and Ageing Study, a community-dwelling sample (n=402), and the Renji Cerebral Small Vessel Disease Cohort Study (RCCS), which consisted of cerebral small vessel disease (CSVD) patients (n=171) and cognitively normal controls (NC) (n=43). DDF was associated with age across all three samples and better explained the variance of changes than other established DWI measures, such as fractional anisotropy, mean diffusivity and peak width of skeletonized mean diffusivity (PSMD). Significant correlations between DDF and cognition were found in the UK Biobank cohort and the MAS cohort. Binary logistic analysis and receiver operator characteristic curve analysis of RCCS demonstrated that DDF had higher sensitivity in distinguishing CSVD patients from NC than the other DWI measures. To demonstrate the flexibility of DDF, we calculated regional DDF which also showed significant correlation with age and cognition. DDF can be used as a marker for monitoring the white matter microstructural changes and ageing related cognitive decline in the elderly.


Assuntos
Envelhecimento/fisiologia , Bases de Dados Factuais , Imagem de Difusão por Ressonância Magnética/métodos , Substância Branca/diagnóstico por imagem , Substância Branca/fisiologia , Idoso , Idoso de 80 Anos ou mais , Cognição/fisiologia , Estudos de Coortes , Estudos Transversais , Bases de Dados Factuais/tendências , Imagem de Difusão por Ressonância Magnética/tendências , Feminino , Humanos , Masculino , Reino Unido/epidemiologia
13.
Front Neurol ; 12: 687959, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34322083

RESUMO

Objectives: We aim to investigate whether multi-dimensional diffusion tensor imaging (DTI) measures can sensitively identify different cognitive status of cerebral small vessel disease (CSVD) and to explore the underlying pattern of white matter disruption in CSVD. Methods: Two hundred and two participants were recruited, composed of 99 CSVD patients with mild cognitive impairment (VaMCI) and 60 with no cognitive impairment (NCI) and 43 healthy subjects as normal controls (NC). Full domain neuropsychological tests and diffusion-weighted imaging were performed on each subject. DTI metrics such as fractional anisotropy (FA), mean diffusivity (MD), the skeletonized mean diffusivity (PSMD), and structural brain network measures including network strength, global efficiency (EGlobal), and local efficiency (ELocal) were calculated. Region of interest (ROI) analysis of 42 white matter tracts was performed to examine the regional anatomical white matter disruption for each group. Results: Significant differences of multiple cognitive test scores across all cognitive domains especially processing and executive function existed among the three groups. DTI measures (FA, MD, and PSMD) showed significant group difference with the cognitive status changing. FA and EGlobal showed significant correlation with processing speed, executive function, and memory. ROI analysis found that white matter integrity impairment occurred from the preclinical stage of vascular cognitive impairment (VCI) due to CSVD. These lesions in the NCI group mainly involved some longitudinal fibers such as right superior longitudinal fasciculus (SLF-R), right superior fronto-occipital fasciculus (SFO-R), and right uncinate fasciculus (UNC-R), which might be more vulnerable to the cerebrovascular aging and disease process. Conclusions: DTI measures are sensitive neuroimaging markers in detecting the early cognitive impairment and able to differentiate the different cognitive status due to CSVD. Subtle changes of some vulnerable white matter tracts may be observed from the preclinical stage of VCI and have a local to general spreading pattern during the disease progression.

14.
J Phys Chem Lett ; 12(28): 6543-6550, 2021 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-34242024

RESUMO

Supercritical carbon dioxide (SC CO2) has shown great potential in fabrication of two-dimensional (2D) amorphous nanomaterials with excellent electric and optical properties, while the amorphization mechanism led by SC CO2 is still unclear. In this work, by investigating the amorphization kinetics of MoO3-x nanomaterials in SC CO2, we find two amorphization mechanisms dependent on the SC CO2 pressure. At lower pressure, forming oxygen vacancies is the dominant effect, while at higher pressure, atomic rearrangement is the controlling factor. Furthermore, we demonstrate that amorphization directly affects the optical performance of MoO3-x nanosheets because of the change in coordination, which further indicates the atomic rearrangement during the amorphization process. Therefore, this work reveals the amorphization mechanism led by SC CO2 and builds a link between amorphization and optical performance; it also provides new inspiration for fabrication of amorphous nanomaterials with tunable optical and photocatalytic performance.

15.
Chem Commun (Camb) ; 57(57): 7011-7014, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-34165122

RESUMO

Developing suitable supports to maximize the atomic utilization efficiency of platinum group metals is of great significance to hydrogen evolution from water splitting. Herein, we report a fully exposed Pt cluster supported on an S-vacancy rich MoS2-x support (Pt/Sv-MoS2-x) by a facile impregnation method. Pt/Sv-MoS2-x exhibits an outstanding electrochemical HER performance with a low overpotential of 26.6 mV at a current density of 10 mA cm-2, a small Tafel slope of 34.8 mV dec-1 and good durability. Most importantly, the mass activity of Pt is an order of magnitude more active than that of commercial Pt/C at an overpotential of 0.08 V. We attribute this exceptional HER catalytic performance to the fact that platinum and Sv-MoS2-x act in synergy to accelerate the reaction kinetics.

16.
Adv Sci (Weinh) ; 8(14): e2100075, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34021718

RESUMO

With the increasing demand for detection accuracy and sensitivity, dual-band polarimetric image sensor has attracted considerable attention due to better object recognition by processing signals from diverse wavebands. However, the widespread use of polarimetric sensors is still limited by high noise, narrow photoresponse range, and low linearly dichroic ratio. Recently, the low-dimensional materials with intrinsic in-plane anisotropy structure exhibit the great potential to realize direct polarized photodetection. Here, strong anisotropy of 1D layered bismuth sulfide (Bi2 S3 ) is demonstrated experimentally and theoretically. The Bi2 S3 photodetector exhibits excellent device performance, which enables high photoresponsivity (32 A W-1 ), Ion /Ioff ratio (1.08 × 104 ), robust linearly dichroic ratio (1.9), and Hooge parameter (2.0 × 10-5 at 1 Hz) which refer to lower noise than most reported low-dimensional materials-based devices. Impressively, such Bi2 S3 nanowire exhibits a good broadband photoresponse, ranging from ultraviolet (360 nm) to short-wave infrared (1064 nm). Direct polarimetric imaging is implemented at the wavelengths of 532 and 808 nm. With these remarkable features, the 1D Bi2 S3 nanowires show great potential for direct dual-band polarimetric image sensors without using any external optical polarizer.

17.
Rice (N Y) ; 14(1): 43, 2021 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-34014427

RESUMO

BACKGROUND: Grain size and weight are important target traits determining grain yield and quality in rice. Wild rice species possess substantial elite genes that can be served as an important resource for genetic improvement of rice. In this study, we identify and validate a novel QTL on chromosome 7 affecting the grain size and weight using introgression lines from cross of Oryza sativa and Oryza minuta. RESULTS: An introgression line 'IL188' has been achieved from a wild species Oryza minuta (2n = 48, BBCC, W303) into O. sativa japonica Nipponbare. The F2 and F2:3 populations derived from a cross between IL188 and Nipponbare were used to map QTLs for five grain size traits, including grain length (GL), grain width (GW), grain length to width ratio (LWR), grain thickness (GT) and thousand grain weight (TGW). A total of 12 QTLs for the five grain traits were identified on chromosomes 1, 2, 3, 6, 7, and 8. The QTL-qGL7 controlling GL on chromosome 7 was detected stably in the F2 and F2:3 populations, and explained 15.09-16.30% of the phenotypic variance. To validate the effect of qGL7, eight residual heterozygous line (RHL) populations were developed through selfing four F2:3 and four F2:4 plants with different heterozygous segments for the target region. By further developing SSR and Indel markers in the target interval, qGL7 was delimited to a ~ 261 kb region between Indel marker Y7-12 and SSR marker Y7-38, which also showed significant effects on grain width and thousand grain weight. Comparing with the reference genome of Nipponbare, stop or frameshift mutations in the exon of the three putative genes LOC_Os07g36830, LOC_Os07g36900 and LOC_Os07g36910 encoding F-box domain-containing proteins may be the candidate genes for qGL7. Scanning electron microscopy analysis of the glume's epidermal cells showed that the cell length and width of NIL-qGL7IL188 was higher than NIL-qGL7Nip, indicating that qGL7 increases grain size and weight by regulating cell expansion. CONCLUSIONS: In this study, we detected 12 QTLs regulating grain size and weight using an introgression line from a cross between Oryza sativa and Oryza minuta. Of these loci, we confirmed and delimited the qGL7 to a ~ 261 kb region. Three putative genes, LOC_Os07g36830, LOC_Os07g36900 and LOC_Os07g36910 encoding F-box domain-containing proteins may be the candidate genes for qGL7. These results provide a basis for map-based cloning of the qGL7 gene and useful information for marker assisted selection in rice grain quality improvement.

18.
J Hum Hypertens ; 2021 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-33976343

RESUMO

The associations between high-density lipoprotein cholesterol (HDL-C) and blood pressure (BP) or hypertension are inconsistent in previous studies. This study aimed to assess these associations in a large cohort of Chinese adults and across different age groups. This cross-sectional association study included 22,081 Chinese adults. Associations of HDL-C with BP and hypertension were analyzed using linear or logistic regression, with or without adjustment for confounding factors. HDL-C was inversely associated with BP and hypertension. These associations were still apparent after adjustment for age, sex, fasting plasma glucose, and low-density lipoprotein cholesterol. Sub-analyses revealed: (1) in the whole cohort and females alone, HDL-C was inversely associated with BP and hypertension in young and middle-aged but not older participants; (2) in males alone, HDL-C was not associated with systolic BP or hypertension. However, HDL-C was either inversely, or not, or positively associated with BP in young, middle-aged, and older males, respectively. After further adjustment for body mass index (BMI), the negative associations of HDL-C with BP and hypertension in the whole cohort became positive ones, and the positive associations only presented in males. These findings suggest that further adjustment for BMI changes inverse associations of HDL-cholesterol with BP and hypertension to positive associations in a cohort of Chinese adults.

19.
J Pharm Biomed Anal ; 199: 114063, 2021 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-33862504

RESUMO

The demand and sales of dietary supplements derived from maca (Lepidium meyenii) have skyrocketed in the last decade and a variety of related nutritional and healthcare products have mushroomed into a business with market prominence. However, the lack of standard testing protocols for quality control could jeopardize the immediate benefits of these products for public health. We describe herein the development of analytical procedures for the determination of glucosinolates (GLs), the biologically active ingredients in maca. Because of the high polarity and instability caused by enzymatic hydrolysis, GLs in maca have been exclusively analyzed using desulfated GLs. This indirect analysis requires additional sample preparation steps, which is labor-intensive, and may lose the original GLs and introduce artificial compounds. Furthermore, the reported GL profiles of maca are inconsistent and incomplete, some GLs may be structurally misidentified. In this context, we focused on direct analysis of intact GLs in maca without the enzymatic desulfation. Four GLs (sinalbin, glucolepigramin, glucolimnanthin, and glucotropaeolin) were identified as the major GLs in maca root powder. An HPLC method based on ion pair chromatography was developed to determine individual and total GLs; chromatographic separations were achieved on a Luna column (C18, 4.6 × 100 mm,3 mm) using 0.1 % TFA in water and in methanol as mobile phase in a gradient elution mode. The developed procedures were validated within the calibration range of 10-500 µg/mL. Inter- and intra-day precision were shown to be lower than 3% at all concentrations levels with recovery between 100.2 % and 103.3 %. The procedures were applied to a total of 42 maca root powder products from 11 manufacturers. Sample analysis revealed a consistent correlation of glucotropaeolin: glucolimnanthin (1: 0.19) across all products with a correlation coefficient of 0.994. The correlation in combination with total GL contents for each product could be used for authentication and GL content determination. Incorporation of the developed procedures into USP monographs will strengthen the public standards for maca powder dietary supplements.


Assuntos
Lepidium , Suplementos Nutricionais , Glucosinolatos , Extratos Vegetais , Pós
20.
Arch Dermatol Res ; 2021 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-33774726

RESUMO

The aim of this review was to evaluate the efficacy and safety of tetracyclines for treatment of pemphigoid. We searched PubMed, EMBASE, Ovid, Web of Science, and the Cochrane Library for studies involving pemphigoid patients treated with tetracyclines published in English before 29 February 2020. References of included studies were also screened to widen the scope of the literature search. Data regarding predefined clinical outcomes of 341 patients from 77 studies were extracted and analyzed. A meta-analysis was conducted on the basis of 4 studies including 2 randomized controlled trials and 2 comparative studies. The patients had a mean age of 74.60 ± 13.18 years, 45.4% were males, and 54.6% were females. There were 185 patients with mild-to-moderate and 143 patients with severe disease. The average initial doses were 1.62 ± 0.39 g/day for tetracycline, 0.20 ± 0.01 g/day for doxycycline, and 0.11 ± 0.05 g/day for minocycline. The average time on tetracyclines was 3.74 ± 5.99 months, and 261 (81.3%) patients reported partial or complete remission. Relapses occurred in 72 (28.3%) cases. Adverse effects were experienced by 130 (41.9%) patients. The pooled ORs for short-term effectiveness, relapse, adverse effects, and 1-year survival in patients treated with oral tetracyclines vs. systemic corticosteroids were 0.40 (95% CI, 0.22-0.76), 0.69 (95% CI, 0.44-1.10), 0.47 (95% CI, 0.27-0.82) and 2.02 (95% CI, 1.16-3.50), respectively. Compared to doxycycline and minocycline, tetracycline was significantly associated with better treatment outcomes and fewer adverse effects (p < 0.05). This review revealed tetracyclines' efficacy and safety in pemphigoid treatment and may offer support for clinical use of tetracyclines in pemphigoid.

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