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1.
Talanta ; 235: 122814, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34517670

RESUMO

Simple and sensitive detection of telomerase activity is of vital importance for both early diagnosis and therapy of malignant tumors. Inspired by DNA-biobarcode amplification reported by Chad A. Mirkin, we developed a facile DNA-biobarcode-like SERS-based copper-mediated signal amplification strategy for sensitive detection of telomerase activity. In this strategy, a duplex DNA constructed by hybridization of a copper oxide nanoparticle (CuO NP)-labeled reporting sequence (RS) with the telomerase primer sequence (TS) is ingeniously designed, and anchored on the magnetic bead (MB) to build the CuO NPs-encoded magnetic bead (MB-CuO NPs) detection probe. Upon selective sensing of telomerase, telomerase elongation reaction and structure change of TS products make the CuO NP-RS displace and separate from MB. The separated CuO NPs are dissolved into a mass of Cu2+, which prompt monodisperse dopamine-functionalized AgNPs (D-AgNPs) signal probe into aggregation, resulting in color changes and significantly enhancing of SERS signal. The SERS signal increases with the increase of Cu2+, which is directly proportional to the telomerase. Benefiting from the transformation of CuO NP to Cu2+ with a high amplification effect, this strategy could realize the telomerase activity measurement down to 3 HeLa cells and a dynamic range of 10-10000 cells. It shows a significant improvement of sensitivity without need for other enzymes and elaborate design, which escapes from the complicated manipulations and design in polymerase chain reaction (PCR) and DNA amplification techniques. Moreover, with this strategy, telomerase activities of different cell lines and telomerase inhibitors screening were successfully performed. Significantly, it can also be utilized for visual detection of telomerase, which validates the potential on-site application and its application as point-of-care testing (POCT) for efficient monitoring. Given the high-performance for telomerase analysis, the strategy has a promising application in biological detection and clinical diagnosis, as well as point-of-care tests.


Assuntos
Técnicas Biossensoriais , Telomerase , Cobre , DNA , Células HeLa , Humanos , Técnicas de Amplificação de Ácido Nucleico , Telomerase/genética , Telomerase/metabolismo
2.
Analyst ; 146(15): 4858-4864, 2021 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-34231571

RESUMO

DBP, one of the phthalic acid esters (PAEs), is known as an endocrine disruptor and is toxic to humans in abnormal concentrations. Here, a high-density and ordered SERS substrate based on the self-assembly of triangular Ag nanoplate (TAgNP) arrays is developed for DBP detection. Benefiting from the ordered arrangement and sharp corners of TAgNPS, the arrays can provide sufficient and uniform hotspots for reproducible and highly active SERS effects. Using Rhodamine 6G (R6G) as a reporter molecule, the SERS enhancement factor (EF) of the TAgNP arrays was found to be as high as 1.2 × 107 and the relative standard deviation was 6.56%. As a trial for practical applications, the TAgNP array substrates were used for the detection of dibutyl phthalate (DBP) in edible oils. In this assay, edible oil samples were added to hexane as an organic phase for the formation of the TAgNP arrays, which caused DBP to be loaded at hotspots. DBP in edible oils could be identified at concentrations as low as 10-7 M. This SERS substrate based on the TAgNP arrays has great potential applications in the high sensitivity and reproducible detection of contaminants in food.


Assuntos
Dibutilftalato , Disruptores Endócrinos , Óleos Vegetais , Prata
3.
Chin J Traumatol ; 24(4): 214-220, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33865671

RESUMO

PURPOSE: Previous studies usually examine the associations between psychological distresses and quality of life (QOL) with a variable-centred approach, while little is known about the effect of the individual variance in time-varying changes of psychological distresses on QOL. Therefore, this study aimed to examine whether individual variance in psychological distresses during the early phases post-earthquake would develop different QOL's levels among adolescent survivors 10-year after the Wenchuan earthquake. METHODS: Data were extracted from the Wenchuan Earthquake Adolescent Health Cohort Study. The current study included 744 adolescent survivors who effectively completed surveys at 6 months, 24 months, and 10 years after the earthquake. Self-report questionnaires were administered to collect information on socio-demographic characteristics, earthquake exposure, life events, anxiety symptoms, depressive symptoms, posttraumatic stress symptoms (PTSS), and QOL. Data were analysed using hierarchical multiple regression. RESULTS: Trajectories of psychological distresses were classified as follow: resistance (anxiety 40.73%; depression 54.70%; PTSS 74.46%), recovery (anxiety 17.20%; depression 9.27%; PTSS 10.35%), delayed dysfunction (anxiety 10.35%; depression 18.15%; PTSS 6.18%), and chronicity (anxiety 31.72%; depression 17.88%; PTSS 9.01%). After controlling covariates, hierarchical multiple regression only revealed that the anxiety trajectory with delayed dysfunction remained significantly predictive for four domains of QOL (physical health, psychological health, social relationships, and environment). CONCLUSION: The current study highlights the importance of focusing on the variations in trajectories of anxiety symptoms among disaster survivors and providing individualized mental health services to improve survivors' QOL.

4.
J Agric Food Chem ; 69(32): 9012-9024, 2021 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-33683118

RESUMO

Echium oil has great nutritional value as a result of its high content of α-linolenic acid (ALA, 18:3ω-3) and stearidonic acid (SDA, 18:4ω-3). However, the comprehensive lipid profiling and exact structural characterization of bioactive polyunsaturated lipids in echium oil have not yet been obtained. In this study, we developed a novel pseudotargeted lipidomics strategy for comprehensive profiling and lipid structural elucidation of polyunsaturated lipid-rich echium oil. Our approach integrated untargeted lipidomics analysis with a targeted lipidomics strategy based on Paternò-Büchi (PB)-tandem mass spectrometry (MS/MS) using 2-acetylpyridine (2-AP) as the reaction reagent, allowing for high-coverage lipid profiling and simultaneous determination of C═C locations in triacylglycerols (TGs), diacylglycerols (DGs), free fatty acids (FFAs), and sterol esters (SEs) in echium oil. A total of 209 lipid species were profiled, among which 162 unsaturated lipids were identified with C═C location assignment and 42 groups of ω-3 and ω-6 C═C location isomers were discovered. In addition, relative isomer ratios of certain groups of lipid C═C location isomers were revealed. This pseudotargeted lipidomics strategy described in this study is expected to provide new insight into structural characterization of distinctive bioactive lipids in food.


Assuntos
Echium , Ácidos Graxos Ômega-3 , Isomerismo , Lipidômica , Lipídeos , Espectrometria de Massas em Tandem
5.
J Agric Food Chem ; 69(32): 9111-9123, 2021 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-33427466

RESUMO

Flax lignans (SDG) and sinapic acid (SA) both have the function of antioxidation and anti-inflammation. However, previous studies have focused mainly on biochemical measurements, gene expression analysis, and clinical assessments. There are limited studies that systematically reveal the underlying mechanism of the anti-inflammation effect of SDG or SA from the lipidomic point of view. Herein, the integrated lipidomic profiling platform was used for the analysis of free fatty acids (FFAs), phospholipids (PLs), triacylglycerols (TAGs), and oxylipins in high-fat (HF)-diet-fed mice after SDG or SA administration. Dietary supplementation of SDG or SA downregulated the levels of total TAGs and FFAs in the ApoE-/- mice model. Furthermore, 28 potential lipids were screened out and considered as key evaluation factors to understand the anti-inflammation function and mechanism of SDG and SA. The results indicated that the anti-inflammatory effect of SDG and SA was principally exerted via regulation of lipid homeostasis.


Assuntos
Linho , Lignanas , Animais , Anti-Inflamatórios , Butileno Glicóis , Ácidos Cumáricos , Dieta Hiperlipídica/efeitos adversos , Glucosídeos , Lipidômica , Camundongos
6.
J Agric Food Chem ; 68(50): 15048-15060, 2020 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-33267575

RESUMO

Dietary supplementation with omega-3 polyunsaturated fatty acids (n-3 PUFAs) for laying hens enriches eggs with these essential fatty acids. However, the enrichment patterns and changes to intact lipids in egg yolk have not been sufficiently revealed. Herein, egg yolk lipids from hens fed with diets supplemented with flaxseed, Schizochytrium sp. residue, or their mixture were comprehensively analyzed using ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). A total of 335 individual lipid species covering 23 (sub)classes were identified and quantified. Distinct n-3 PUFA-lipid profiles were revealed among different groups. Dietary α-linolenic acid (ALA) was mainly deposited in the TAG fraction, whereas synthesized or preformed docosahexaenoic acid (DHA) predominantly existed in the glycerophospholipid form. Furthermore, different lipid species were identified and related lipid pathways after dietary supplementation were analyzed. Collectively, these findings provide us with new knowledge for production, nutritional evaluation, authentication, and application of n-3 PUFA-enriched eggs.


Assuntos
Ração Animal/análise , Galinhas/metabolismo , Gema de Ovo/química , Ácidos Graxos Ômega-3/metabolismo , Animais , Dieta/veterinária , Gema de Ovo/metabolismo , Feminino , Óleo de Semente do Linho/análise , Óleo de Semente do Linho/metabolismo , Lipidômica
7.
Anal Chem ; 92(9): 6446-6455, 2020 05 05.
Artigo em Inglês | MEDLINE | ID: mdl-32250592

RESUMO

Free fatty acids (FFAs) are key intermediates of lipid metabolism that have a crucial role in many critical biological processes. The specific locations of carbon-carbon double bonds (C═C) in FFAs are often associated with distinct biological functions. Despite the rapid development of analytical techniques, identification of C═C locations in FFAs with more than three C═C bonds in complex biological matrices remains a challenge. Herein, we describe a double derivatization strategy, coupled with shotgun-mass spectrometry (MS), for unambiguous and sensitive determination of a high-coverage C═C bond (from 1 to 6) locations of FFAs. Our approach is based on combination of acetone labeling of C═C bonds and N,N-diethyl-1,2-ethanediamine (DEEA) labeling of carboxyl groups within FFAs. Acetone labeling of C═C bonds via photochemical reaction provides diagnostic ions, specific to C═C locations, and DEEA labeling of carboxyl groups significantly enhances MS response of diagnostic ions, by invoking a readily protonated tertiary amine group on FFA analytes. By exploiting this double derivatization strategy, the assignment of C═C locations of FFAs with more than three C═C bonds was achieved with high sensitivity (limit of quantitation (LOQ) 0.1-1.5 nmol/L). In contrast, such assignments were not possible by acetone labeling alone, because of the low sensitivity of diagnostic ions in negative ionization mode of MS. The applicability of our method was demonstrated by profiling of FFAs, including unsaturated FFA C═C positional isomers, in liver samples from mice with nonalcoholic fatty liver disease (NAFLD) and their lean controls. The study showed that the high-specificity and high-sensitivity method developed here is promising for accurate identification and quantitation of a wide array of FFAs in biological samples.

8.
Chem Commun (Camb) ; 56(31): 4288-4291, 2020 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-32182314

RESUMO

The tough challenges for the sensitive and accurate detection of circulating miRNAs (cmiRNAs) in the peripheral blood are low abundance, and high interference from environmental or background factors or other molecules. Here, we developed a double-signal mode based on metal-organic framework coupled cascaded nucleic acid circuits for the accurate and sensitive detection of serum circulating miRNAs.


Assuntos
Técnicas Biossensoriais/métodos , MicroRNA Circulante/sangue , Sondas de DNA/química , DNA/química , Estruturas Metalorgânicas/química , MicroRNA Circulante/genética , DNA/genética , Sondas de DNA/genética , Corantes Fluorescentes/química , Glucose Oxidase/química , Humanos , Peróxido de Hidrogênio/química , Limite de Detecção , MicroRNAs/sangue , MicroRNAs/genética , Hibridização de Ácido Nucleico , Oxirredução , Fenazinas/química , Fenilenodiaminas/química , Espectrometria de Fluorescência/métodos
9.
Food Chem ; 319: 126547, 2020 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-32182541

RESUMO

In this research, a novel ultrasound-assisted extraction (USAE) method using a one-phase solvent (isopropanol, IPA) combined with liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QToF-MS) was developed for exhaustive profiling of egg yolk lipids, which are considered to be essential nutrients that have important functions for human health. Compared with IPA-vortex extraction method, the optimized IPA-USAE method shows better performance in terms of extraction time, lipid losses, lipid recoveries and the number of lipids identified. With this established simple, high throughput and reliable analytical approach, untargeted comprehensive lipid profiling of four kinds of egg yolks was achieved, thus providing a powerful tool for nutritional research of egg lipids. Considering that the lipids contained in egg yolk are quite abundant in both quantity and species, the proposed IPA-USAE combined with LC-ESI-QToF-MS is also potentially applicable to comprehensive extraction and profiling of lipids in other biological samples.


Assuntos
Gema de Ovo/química , Lipídeos/química , 2-Propanol/química , Cromatografia Líquida de Alta Pressão , Solventes/química , Espectrometria de Massas por Ionização por Electrospray , Ultrassom
10.
Nanomaterials (Basel) ; 10(2)2020 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-32093184

RESUMO

Among the transition metal oxides, the Mn3O4 nanostructure possesses high theoretical specific capacity and lower operating voltage. However, the low electrical conductivity of Mn3O4 decreases its specific capacity and restricts its application in the energy conversion and energy storage. In this work, well-shaped, octahedron-like Mn3O4 nanocrystals were prepared by one-step hydrothermal reduction method. Field emission scanning electron microscope, energy dispersive spectrometer, X-ray diffractometer, X-ray photoelectron spectrometer, high resolution transmission electron microscopy, and Fourier transformation infrared spectrometer were applied to characterize the morphology, the structure, and the composition of formed product. The growth mechanism of Mn3O4 nano-octahedron was studied. Cyclic voltammograms, galvanostatic charge-discharge, electrochemical impedance spectroscopy, and rate performance were used to study the electrochemical properties of obtained samples. The experimental results indicate that the component of initial reactants can influence the morphology and composition of the formed manganese oxide. At the current density of 1.0 A g-1, the discharge specific capacity of as-prepared Mn3O4 nano-octahedrons maintains at about 450 mAh g-1 after 300 cycles. This work proves that the formed Mn3O4 nano-octahedrons possess an excellent reversibility and display promising electrochemical properties for the preparation of lithium-ion batteries.

11.
J Psychosom Res ; 129: 109870, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31862630

RESUMO

OBJECTIVE: The multi-wave longitudinal study was to explore the underlying mechanisms between sleep disturbance and depressive symptoms for up to 9.5 months among student nurses and to examine whether emotional exhaustion and sleep-related worry could be the mediators. METHODS: 856 student nurses aged 15-24 years (mean age = 17.8 years; all female) were recruited from a nursing school. Participants reported demographic variables (age, sibling, location, health condition, family monthly income and parents' marital quality) at baseline (Time1), Body Mass Index, weekly exercise, sleep disturbance and depressive symptoms at 3-month into internship (Time2), emotional exhaustion and sleep-related worry at 6-month into internship (Time3), and depressive symptoms at 9.5-month follow-up (Time4). Mediation analyses with bootstrapping were conducted to investigate the mediating role of emotional exhaustion and sleep-related worry in the relationship between sleep disturbance and depressive symptoms. RESULTS: Adjusted analyses suggested that the direct effect of sleep disturbance on depressive symptoms was not significant (ß = 0.026, SE = 0.069, p = .71). Mediation analyses revealed two significant indirect effects between Time2 sleep disturbance and Time4 depressive symptoms with the mediating effect of emotional exhaustion (ß = 0.019, SE = 0.009, BC95%CI [0.0036,0.0405]) and sleep-related worry (ß = 0.016, SE = 0.008, BC95%CI [0.0026,0.0337]) respectively. When testing serial multiple mediation, the specific indirect effect of sleep disturbance on depressive symptoms through both emotional exhaustion and sleep-related worry was significant (ß = 0.005, SE = 0.003, BC95%CI [0.0004,0.0123]). CONCLUSIONS: Emotional exhaustion and sleep-related worry were positively associated with sleep disturbance and depressive symptoms.


Assuntos
Depressão/psicologia , Enfermeiras e Enfermeiros/psicologia , Transtornos do Sono-Vigília/etiologia , Estudantes/psicologia , Adolescente , Adulto , Feminino , Humanos , Estudos Longitudinais , Transtornos do Sono-Vigília/psicologia , Adulto Jovem
12.
Mikrochim Acta ; 186(8): 562, 2019 07 23.
Artigo em Inglês | MEDLINE | ID: mdl-31338602

RESUMO

A simple, rapid and ultrasensitive surface-enhanced Raman scattering (SERS) assay was developed for detection of Cu2+. It is based on the use of polyvinylpyrrolidine-functionalized silver nanoparticles (AgNPs). Detection is enabled because of the presence of N and O atoms in PVP which have specific coordination capability for Cu2+. Coordination is accompanied by the appearance of the change in the intensity ratio of the 845 cm-1 and 899 cm-1 peaks (I845/I899) that is associated with pyrrolidinone ring torsion in PVP. The intensity of the band increases linearly in the 0.01 to 2 µM Cu2+ concentration range, and the limit of detection is as low as 3 nM. The assay potentially is a promising tool for monitoring of Cu2+ in environmental water. Graphical abstract Schematic representation of a method for determination of Cu2+ using polyvinylpyrrolidone-functionalized AgNPs as a SERS nanoprobe. Cu2+ can coordinate with PVP through the N and O atoms. This effect induces AgNP aggregation and affects the electronic structure of PVP. The changes of the Raman spectra are proportional to the Cu2+ concentration.

13.
Food Chem ; 285: 194-203, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-30797335

RESUMO

Microwave pretreatment of rapeseeds prior to cold-pressing is a simple and desirable method for producing high quality oils. In this study, a rapid and sensitive lipid profiling platform employing an accurate quantification strategy was established based on direct infusion electrospray ionization tandem mass spectrometry. Using this developed platform, we further investigated the effect of microwave pretreatment of rapeseeds on the contents of important lipids such as triglycerides (TAGs), phospholipids (PLs), and free fatty acids (FFAs) in 15 different rapeseed oils. Our results demonstrated that no significant changes of total FAs and TAGs contents were observed after microwave pretreatment, while FFA contents increased and PLs contents significantly increased up to 40 folds. The potential mechanism of lipid changes was also discussed. The established lipidomics profiling platform can provide reliable lipids profiling data and help to better understand the potential mechanism of microwave pretreatment in oil processing.


Assuntos
Lipídeos/análise , Óleo de Brassica napus/análise , Espectrometria de Massas por Ionização por Electrospray/métodos , Brassica rapa/química , Brassica rapa/metabolismo , Cromatografia Gasosa , Ácidos Graxos não Esterificados/análise , Micro-Ondas , Fosfolipídeos/análise , Óleos Vegetais/análise , Óleos Vegetais/química , Óleo de Brassica napus/química , Temperatura , Triglicerídeos/análise
14.
J Inorg Biochem ; 189: 58-68, 2018 12.
Artigo em Inglês | MEDLINE | ID: mdl-30243119

RESUMO

Oxidative stress accompanies various diseases associated with chronic inflammation. In this work, H2O2 and H2O2-Fe3O4 magnetic nanoparticles were used as two reactive oxygen species to study the oxidative stress for the structure and polymerization behaviour of fibrinogen molecules. The alterations of secondary structure and component of fibrinogen molecule were characterized by circular dichroism spectra, ultraviolet-visible spectra and fluorescence spectra, the viscoelasticity of fibrinogen solution was studied by dynamic light scattering microrheology. Based on the molecular dynamics simulations and fluorescence properties, the possible oxidative stress sites were analyzed and confirmed by Tb3+ probe. The hydrophobicity/philicity and electrostatic net charges present on the exterior part of the fibrinogen molecules were measured with zeta potential. The height and image analysis obtained from atomic force microscope indicated that oxidative stress of fibrinogen molecules could influence the equilateral junctions of protofibrils and the different cross-linking patterns between the α- and γ-chains, result in the decrease of the fibre size, form a higher proportion of branching and a denser aggregation state. This study will provide insights into the misfolding and fibril formation of disease-associated fibrinogen, facilitate an increased understanding of how oxidative stress in vivo affects the formation and polymerization of fibrin, and support efforts for the improved treatment of patients suffering from the thrombotic disease.


Assuntos
Fibrina/química , Fibrinogênio/química , Nanopartículas de Magnetita/química , Humanos , Microscopia de Força Atômica , Simulação de Dinâmica Molecular , Oxirredução , Estresse Oxidativo
15.
Food Funct ; 9(10): 5103-5114, 2018 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-30207361

RESUMO

Atherosclerosis is a chronic disease associated with oxidative stress and inflammatory activation and is the main underlying trigger for cardiovascular disease. There are many cardiovascular health products in the market; in order to evaluate the effect of these products, in this paper, a novel lipid profiling platform was established using the shot-gun mass spectrum method for the analysis of free fatty acid and phospholipids, and the high performance liquid chromatography coupled with mass spectroscopy method for the analysis of lipid mediators and triacylglycerol, respectively, in serum from male apolipoprotein E-knock out mice after different diet interventions. Changes in the four lipids above, and pathways and regulation of lipid metabolism in mice from different groups were further investigated. The result showed that all cardiovascular health products showed some certain potential to alleviate atherogenesis and ameliorate inflammation; among them, lemon apple cider vinegar drink and seal oil could significantly decrease triacylglycerol in mouse serum. The establishment of this lipidome profiling platform helps to better understand the metabolism regulation and intervention mechanism of different cardiovascular health products in chronic diseases, such as atherogenesis. This platform could be applied to other cardiovascular health products and provide reliable lipid profiling data for their potential effect.


Assuntos
Apolipoproteínas E/deficiência , Aterosclerose/dietoterapia , Metabolismo dos Lipídeos , Lipídeos/sangue , Animais , Apolipoproteínas E/genética , Aterosclerose/sangue , Aterosclerose/genética , Aterosclerose/metabolismo , Dieta , Modelos Animais de Doenças , Humanos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Estresse Oxidativo
16.
Electrophoresis ; 39(13): 1558-1568, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-29572876

RESUMO

Vegetable oils and animal fats are dietary source of lipids that play critical and multiple roles in biological function. Triacylglycerols (TAGs) are the principal component of oils and fats with significant difference in profile among different oils and fats. TAG profiling is essential for nutritional evaluation, quality control and assurance of safety in oils and fats. However, analysis of TAGs is a challenging task because of the complicated composition of TAGs and their similar physicochemical properties in oils and fats. The rapid development of mass spectrometry (MS) technology in recent years makes it possible to analyze the composition, content and structure of TAGs in the study of the physical, chemical and nutritional properties of oils, fats and related products. This review described the research advancement based on MS for profiling of TAGs in oil, fat and their applications in food. The application of MS, including direct infusion strategies, and its combination with chromatography, gas chromatography-MS (GC-MS) and liquid chromatography-MS (LC-MS), in the analysis of TAGs were reviewed. The advantages and disadvantages of these analytical methods with relevant applications for TAGs analysis in food were also described.


Assuntos
Gorduras/química , Óleos Vegetais/química , Triglicerídeos/análise , Cromatografia , Análise de Alimentos/métodos , Qualidade dos Alimentos , Humanos , Espectrometria de Massas , Análise de Componente Principal
17.
Pak J Pharm Sci ; 29(1 Suppl): 321-3, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-27005498

RESUMO

UNLABELLED: This study was to research the incidence of infants with rotavirus enteritis combined with lactose intolerance and the clinical effect of low lactose milk powder for infantile rotavirus enteritis with lactose intolerance. The control groups were 126 cases of infants with diarrhea randomly collected from our hospital at the same period, which their rotavirus detection was negative. The observation group was 185 cases of infants with rotavirus, which was tested to be positive. Through the urine galactose determination, 62 cases of the control group were positive and 124 cases of the observation group were positive. Then 124 cases of infants with rotavirus combined with lactose intolerance were randomly divided into two groups. 60 cases in the control group were given rehydration, correction of acidosis, oral smecta, Intestinal probiotics and other conventional treatment, then continued to the original feeding method. While, 64 cases in the treatment group, on the basis of routine treatment, applied the low lactose milk feeding. To observe the total effective rate for the two groups. The incidence of lactose intolerance in children with rotavirus enteritis (67.03%) was significantly higher than that of children with diarrhea (49.2%), which was tested to be negative. And the difference was statistically significant (p<0.5). In the aspect of reducing the frequency of diarrhea, and diarrhea stool forming time, the treatment group has the obvious superiority. The total effective rate was 95.4% for treatment group, which was higher than that in the control group (76.7%), the difference was statistically significant (P<0.05). CONCLUSION: Infants with rotavirus enteritis was easier to merge with lactose intolerance. The low lactose milk powder could improve the therapeutic effectively and could reduce the duration of disease, and restored to normal diet for 2 weeks feeding time.


Assuntos
Enterite/dietoterapia , Enterite/epidemiologia , Intolerância à Lactose/dietoterapia , Intolerância à Lactose/epidemiologia , Infecções por Rotavirus/dietoterapia , Infecções por Rotavirus/epidemiologia , Acidose Láctica/dietoterapia , China/epidemiologia , Laticínios , Diarreia/epidemiologia , Diarreia/etiologia , Enterite/etiologia , Feminino , Humanos , Incidência , Lactente , Recém-Nascido , Intolerância à Lactose/complicações , Masculino , Probióticos/uso terapêutico , Infecções por Rotavirus/complicações , Resultado do Tratamento
18.
Pak J Pharm Sci ; 29(1 Suppl): 361-5, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-27005503

RESUMO

2124 neonates were monitored from February 2013 to August 2014, among which 1119 were admitted from outpatient department (outpatient group), 782 were transferred from other departments (other department group), and 223 were from other hospitals (other hospital group). Through it we explore the distribution of multidrug resistant organism in neonates, which were admitted to the hospital through various ways, and therefore analyze the risk factors of nosocomial infection to avoid cross infection of multi drug resistant organism in neonatology department. The results showed that 105 strains of multi drug resistant bacteria were detected in the neonatal department. Among them, there were 57 strains from the outpatient group, 27 from the other department group, and 21 from the other hospital group. Neonates with the hospitalization time of more than 14 days and low birth weight infants (1500 g) were the high-risk groups of drug-resistant strains in nosocomial infection. So the infection in neonates from other departments or hospitals should be strengthen, especially the prevention and control in neonates with the hospitalization time than 14 days and low birth weight infants (1500 g) in order to reduce the occurrence of multiple drug-resistant strains cross infection.


Assuntos
Infecção Hospitalar/microbiologia , Infecção Hospitalar/prevenção & controle , Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos , Bactérias/efeitos dos fármacos , Peso ao Nascer , Humanos , Recém-Nascido de Baixo Peso , Recém-Nascido , Tempo de Internação , Fatores de Risco
19.
Zhongguo Zhen Jiu ; 36(10): 1027-1030, 2016 Oct 12.
Artigo em Chinês | MEDLINE | ID: mdl-29231520

RESUMO

OBJECTIVE: To explore the efficacy of local acupuncture therapy on post-stroke pseudo-bulbar palsy and the clinical advantageous protocol of local acupuncture therapy. METHODS: Eighty patients of post-stroke pseudo-bulbar palsy were randomized into a quick needle insertion group and a routine acupuncture group, 40 cases in each one. The western medicine, such as thrombolysis, lipid regulation, antiplatelet aggregation, antihypertension and hypoglycemic therapy method was all used in the two groups. On the basis of the treatment of western medicine, in the quick needle insertion group, the perpendicular needle insertion was used at Aqiang point, about 8 to 12 mm in depth. When the emptiness feeling presented under the needle, the needle went slowly for 2 mm more depth till cough occurred, and removed afterward. The treatment was given once every day, and totally 20 treatments were required. In the routine acupuncture group, Lianquan (CV 23) was stimulated. The needle was inserted toward the tongue root, about 40 mm in depth. The needle was rotated till the patient felt soreness and distention at the tongue root, and then retained for 30 min. The treatment was given once a day, and totally 20 treatments were required. The water swallow test score and clinical efficacy were evaluated before and after treatment. RESULTS: The curative rate was 80.0% (32/40) in the quick needle insertion group, better than 55.0% (22/40) in the routine acupuncture group (P<0.05). The total effective rate was 97.5% (39/40) in the quick needle insertion group and was 90.0% (36/40) in the routine acupuncture group, indicating no significant difference in comparison (P>0.05). The water swallow test scores decreased after treatment as compared with those before treatment in the two groups (both P<0.01), and the water swallow test scores after treatment of the two groups had no significant difference (P>0.05). CONCLUSIONS: Acupuncture at local point is effective for post-stroke pseudo-bulbar palsy.The curative rate of quick needle insertion at Aqiang point is better than routine acupuncture at Lianquan (CV 23).


Assuntos
Pontos de Acupuntura , Paralisia Bulbar Progressiva/terapia , Acidente Vascular Cerebral/complicações , Terapia por Acupuntura/métodos , Paralisia Bulbar Progressiva/etiologia , Transtornos de Deglutição/terapia , Humanos , Terapia Trombolítica/métodos , Resultado do Tratamento
20.
Analyst ; 140(22): 7637-44, 2015 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-26421322

RESUMO

A fluorescence magnetic biosensor for the DNA methyltransferase activity was developed based on the cooperative amplification by combining the magnetic nanoparticles synergistic exonuclease III (Exo III)-assisted circular exponential amplification and a supramolecular structure ZnPPIX/G-quadruplex. First, a duplex DNA probe, which was constructed by the hybridization of a quadruplex-forming oligomer with a molecular beacon, was assembled on the magnetic nanoparticles (MNPs) as a reporter. A hairpin probe (HP)-containing sequence of GATC was used as the methylation substrate of DNA adenine methyltransferase (DAM). Once HP was methylated by DAM, it could be recognized and cleaved by Dpn I, which allows the release of a single-stranded DNA. The DNA (tDNA1) then hybridizes to the MNP probe, which then triggers the exonuclease III-mediated target exponential recycling reaction. Simultaneously, numerous quadruplex forming oligomers are liberated and folded into the G-quadruplex-ZnPPIX complexes with the help of zinc(ii)-protoporphyrin IX(ZnPPIX) on the MNP surface to give a remarkable fluorescence response. In the developed sensor, a small amount of target DAM can be converted to a large number of stable DNA triggers, leading to remarkable amplification of the target. Moreover, using MNPs as a vector of the sensor may reduce the interference from the real samples, which increases the anti-interference of the sensing system. Based on this unique amplification strategy, a very low detection limit down to 2.0 × 10(-4) U mL(-1) was obtained. Furthermore, the sensor could be used to evaluate the DAM activity in different growth stages of E. coli cells and screen Dam MTase inhibitors. Therefore, the strategy proposed here provides a promising platform for monitoring the activity and inhibition of DNA MTases and has great potential to be applied further in early clinical diagnostics and medical research.


Assuntos
Metilases de Modificação do DNA/metabolismo , Ensaios Enzimáticos/métodos , Escherichia coli/enzimologia , Exodesoxirribonucleases/metabolismo , Corantes Fluorescentes/química , Nanopartículas de Magnetita/química , Metilases de Modificação do DNA/análise , Escherichia coli/química , Escherichia coli/metabolismo , Quadruplex G , Espectrometria de Fluorescência/métodos
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