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1.
J Inflamm Res ; 14: 5337-5347, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34703272

RESUMO

Purpose: Clinically, it is challenging to manage diabetic patients with periodontitis. Biochemically, both involve a wide range of inflammatory/collagenolytic conditions which exacerbate each other in a "bi-directional manner." However, standard treatments for this type of periodontitis rely on reducing the bacterial burden and less on controlling hyper-inflammation/excessive-collagenolysis. Thus, there is a crucial need for new therapeutic strategies to modulate this excessive host response and to promote enhanced resolution of inflammation. The aim of the current study is to evaluate the impact of a novel chemically-modified curcumin 2.24 (CMC2.24) on host inflammatory response in diabetic rats. Methods: Type I diabetes was induced by streptozotocin injection; periodontal breakdown then results as a complication of uncontrolled hyperglycemia. Non-diabetic rats served as controls. CMC2.24, or the vehicle-alone, was administered by oral gavage daily for 3 weeks to the diabetics. Micro-CT was used to analyze morphometric changes and quantify bone loss. MMPs were analyzed by gelatin zymography. Cell function was examined by cell migration assay, and cytokines and resolvins were measured by ELISA. Results: In this severe inflammatory disease model, administration of the pleiotropic CMC2.24 was found to normalize the excessive accumulation and impaired chemotactic activity of macrophages in peritoneal exudates, significantly decrease MMP-9 and pro-inflammatory cytokines to near normal levels, and markedly increase resolvin D1 (RvD1) levels in the thioglycolate-elicited peritoneal exudates (tPE). Similar effects on MMPs and RvD1 were observed in the non-elicited resident peritoneal washes (rPW). Regarding clinical relevance, CMC2.24 significantly inhibited the loss of alveolar bone height, volume and mineral density (ie, diabetes-induced periodontitis and osteoporosis). Conclusion: In conclusion, treating hyperglycemic diabetic rats with CMC2.24 (a tri-ketonic phenylaminocarbonyl curcumin) promotes the resolution of local and systemic inflammation, reduces bone loss, in addition to suppressing collagenolytic MMPs and pro-inflammatory cytokines, suggesting a novel therapeutic strategy for treating periodontitis complicated by other chronic diseases.

2.
Am J Orthod Dentofacial Orthop ; 160(6): 805-813, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34344557

RESUMO

INTRODUCTION: In this study, we compared achieved and predicted crown movements of maxillary and mandibular first molars, canines, and central incisors in adults after 4 first premolar extraction treatment with Invisalign. METHODS: Seventeen adult patients who received 4 first premolar extraction treatment with Invisalign and completed the first series of aligners were included. Superimposition of pretreatment and actual posttreatment dental models was acquired using registrations of pretreatment and posttreatment craniofacial models on the basis of bone surfaces and registrations of craniofacial and dental models on the basis of dental crown surfaces, respectively. Superimposition of pretreatment and predicted posttreatment models was acquired from ClinCheck software. Achieved and predicted 3-dimensional crown movements of maxillary and mandibular first molars, canines, and central incisors were then compared using the paired t test. RESULTS: Relative to predicted changes, first molars achieved greater mesial displacement, mesial tipping, and buccal inclination in both the maxilla and mandible, greater intrusion in the maxilla, and greater mesial-lingual rotation and less constriction in the mandible. Canines achieved greater distal tipping in both the maxilla and mandible, less retraction in the maxilla, and greater lingual inclination and extrusion in the mandible. Central incisors achieved greater distal tipping and lingual inclination and extrusion in both the maxilla and mandible and less retraction in the maxilla. CONCLUSIONS: Tooth crown movements were not fully achieved as predicted following Invisalign treatment. Differences focused on mesial tipping, buccal inclination, mesial displacement, and intrusion of the first molars, as well as distal tipping, lingual inclination, insufficient retraction, and intrusion of the canines and central incisors.


Assuntos
Aparelhos Ortodônticos Removíveis , Coroa do Dente , Adulto , Dente Pré-Molar/cirurgia , Coroas , Humanos , Incisivo , Mandíbula , Maxila , Técnicas de Movimentação Dentária
3.
Am J Orthod Dentofacial Orthop ; 159(5): e423-e437, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33653638

RESUMO

INTRODUCTION: The objective of this research was to evaluate the correlation between 3-dimensional (3D) lip vermilion (LV) morphology and skeletal patterns as well as incisor measurements in young Chinese adults. METHODS: In all, 240 young adults were enrolled; these included 80 patients each with skeletal Class I, Class II, and Class III malocclusions, respectively. Each sagittal skeletal pattern included 40 male and 40 female subjects. Twenty-two 3D LV measurements were obtained from 3D facial scans. Skeletal and incisor measurements were evaluated on lateral cephalograms. Correlation and regression analysis were performed between soft and hard tissue measurements. RESULTS: Six of 22 LV measurements showed significant differences between male and female subjects. The 3D LV morphology showed significant differences with respect to different skeletal patterns and sex. Adults with skeletal Class III malocclusion tended to have thinner upper vermilion and fuller lower vermilion than subjects with skeletal Class II and III malocclusion. The mandibular plane angle negatively correlated with the upper-lower vermilion midsagittal curve length and surface area ratio in adults with skeletal Class I and II malocclusion, yet the vertical facial skeletal type showed no correlation in adults with skeletal Class III malocclusion. The vermilion angle, central bow angle, vermilion height, vermilion midsagittal curve length, vermilion height and width ratio, and vermilion surface area showed a significant correlation with incisor measurements. Regression analysis found that the ANB angle was an important factor affecting the upper and lower vermilion midsagittal curve length and surface area ratio. Further, the vermilion height and height and width ratio were closely correlated with the interincisal (U1/L1) angle, whereas the central bow angle was closely correlated with the maxillary incisor torque. CONCLUSIONS: Most LV morphology variables were correlated to skeletal patterns and incisor measurements. Skeletal Class III malocclusion showed significant differences in vermilion morphology. Both the sagittal and vertical skeletal pattern have effects on vermilion proportion. The incisor torque was closely correlated to vermilion shape and central bow angle and might influence the vermilion esthetics. However, the proportion of the upper and lower vermilion was mainly affected by the ANB angle.


Assuntos
Má Oclusão Classe III de Angle , Má Oclusão Classe II de Angle , Cefalometria , China , Estética Dentária , Feminino , Humanos , Lábio/diagnóstico por imagem , Masculino , Má Oclusão Classe II de Angle/diagnóstico por imagem , Má Oclusão Classe III de Angle/diagnóstico por imagem , Mandíbula/diagnóstico por imagem , Adulto Jovem
4.
Head Face Med ; 17(1): 9, 2021 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-33741006

RESUMO

BACKGROUND: 3D facial scanning has changed the way facial aesthetic is evaluated and has numerous advantages for facial analysis. The specific relationship between lip vermilion morphological changes after orthodontic extraction treatment has not been fully explained. The objective of this study was to evaluate 3D morphological changes after orthodontic extraction treatment in lip vermilion of adult females with dentoalveolar protrusion using a structured light-based scanner. METHODS: Forty-two female subjects (25.2 ± 1.9 years) were recruited as the treatment group; these patients had undergone extraction treatment and achieved better sagittal profiles. Twenty female subjects (25.5 ± 2.1 years) were enrolled in the non-treatment group; these patients did not require any orthodontic treatment. The follow up time for the treatment group was more than 24 months and for the non-treatment group was more than 12 months. 3D facial scans were captured using 3D CaMega. Six landmarks (Ls, Li, R.Chp, L.Chp, R.Ch, and L.Ch), three linear measurements (mouth height, philtrum width, and mouth width), and three area measurements (upper, lower, and total vermilion area) were measured. The spatial deviations of three volumetric measurements (upper, lower, and total vermilion) were constructed for quantitative analysis. Color-coded displacement map were constructed for visualization of the soft-tissue displacement as qualitative evaluation. RESULTS: Mouth height and philtrum width decreased (-0.93 mm and - 1.08 mm, respectively) significantly (p = 0.008 and p = 0.027, respectively), and no significant (p = 0.488) change in mouth width was observed in the treatment group. The lower and total vermilion surface areas decreased (-51.00mm2 and - 69.82mm2, respectively) significantly (p = 0.003 and p = 0.031, respectively) in the treatment group, but no statistically significant (p = 0.752) change was detected in the upper vermilion. In the treatment group, significant retractions were observed in the color-coded displacement map, and three volumetric measurements of vermilion changed significantly (p = 0.012, p = 0.001 and p = 0.004, respectively). Significant differences were found between the treatment group and the non-treatment group in the linear, area and volumetric measurements. CONCLUSIONS: This study established a method for qualitative and quantitative evaluation of the lip vermilion. Significant 3D retraction of the lip vermilion after the extraction treatment was found, with morphological variation between upper and lower vermilion.


Assuntos
Face , Lábio , Adulto , Cefalometria , Feminino , Humanos , Lábio/anatomia & histologia , Lábio/diagnóstico por imagem , Estudos Longitudinais , Estudos Retrospectivos
5.
Biomed Pharmacother ; 131: 110607, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32841898

RESUMO

Epigenetic mechanisms, such as acetylation, methylation, and succinylation, play pivotal roles in the regulation of multiple normal biological processes, including neuron regulation, hematopoiesis, bone cell maturation, and metabolism. In addition, epigenetic mechanisms are closely associated with the pathological processes of various diseases, such as metabolic diseases, autoimmune diseases and cancers. Epigenetic changes may precede genetic mutation, so research on epigenetic changes and regulation may be important for the early detection and diagnosis of disease. Histone deacetylase11 (HDAC11) is the newest member of the histone deacetylase (HDAC) family and the only class IV histone deacetylase. HDAC11 has different expression levels and biological functions in different systems of the human body and is among the top 1 to 4% of genes overexpressed in cancers, such as breast cancer, hepatocellular carcinoma and renal pelvis urothelial carcinoma. This article analyzes the role and mechanism of HDAC11 in disease, especially in tumorigenesis, in an attempt to provide new ideas for clinical and basic research.


Assuntos
Epigênese Genética/fisiologia , Inibidores de Histona Desacetilases/farmacologia , Histona Desacetilases/genética , Histona Desacetilases/metabolismo , Animais , Epigênese Genética/efeitos dos fármacos , Redes Reguladoras de Genes/efeitos dos fármacos , Redes Reguladoras de Genes/fisiologia , Hematopoese/efeitos dos fármacos , Hematopoese/fisiologia , Inibidores de Histona Desacetilases/uso terapêutico , Humanos , Neoplasias/tratamento farmacológico , Neoplasias/genética , Neoplasias/metabolismo
6.
Virol J ; 17(1): 80, 2020 06 19.
Artigo em Inglês | MEDLINE | ID: mdl-32560646

RESUMO

BACKGROUND: Convalescent plasma (CP) transfusion was reported to be effective in treating critically ill patients with COVID-19, and hydroxychloroquine could potently inhibit SARS-CoV-2 in vitro. Herein, we reported a case receiving combination therapy with CP transfusion and hydroxychloroquine for the first time. CASE PRESENTATION: Laboratory findings showed high lactic acid level (2.1 mmol/L) and C-reactive protein (CRP, 48.8 mg/L), and low white blood cell count (1.96 × 109/L) in a 65-year-old Chinese man, who was diagnosed with severe COVID-19. CP was intravenously given twice, and hydroxychloroquine was orally administrated for a week (0.2 g, three times a day). The lactic acid and C-reactive protein levels remained high (2.1 mmol/L and 73.23 mg/L, respectively), while the arterial oxyhemoglobin saturation decreased to 86% with a low oxygenation index (OI, 76 mmHg) on day 4 after CP transfusion. His temperature returned to normal and the OI ascended above 300 on day 11. Moreover, the RNA test remained positive in throat swab, and computed tomography revealed severe pulmonary lesions on day 11 after admission. CONCLUSION: These findings suggested that the effectiveness of combination therapy with CP and hydroxychloroquine may be non-optimal, and specific therapy needs to be explored.


Assuntos
Transfusão de Componentes Sanguíneos/métodos , Infecções por Coronavirus/terapia , Hidroxicloroquina/administração & dosagem , Pneumonia Viral/terapia , Administração Oral , Idoso , Anticorpos Neutralizantes/administração & dosagem , Anticorpos Antivirais/administração & dosagem , Betacoronavirus/isolamento & purificação , Proteína C-Reativa/metabolismo , COVID-19 , Infecções por Coronavirus/sangue , Infecções por Coronavirus/tratamento farmacológico , Infecções por Coronavirus/imunologia , Infecções por Coronavirus/virologia , Humanos , Imunização Passiva/métodos , Ácido Láctico/sangue , Contagem de Leucócitos , Masculino , Oxiemoglobinas , Pandemias , Pneumonia Viral/sangue , Pneumonia Viral/tratamento farmacológico , Pneumonia Viral/virologia , SARS-CoV-2 , Carga Viral
7.
Sci Rep ; 9(1): 14549, 2019 10 10.
Artigo em Inglês | MEDLINE | ID: mdl-31601925

RESUMO

We assessed the three-dimensional (3D) pattern of the physiologic drift of the remaining adjacent teeth after premolar extraction due to orthodontic reasons and the associated factors. Data were collected from 45 patients aged 17.04 ± 5.14 years who were scheduled to receive a fixed appliance after maxillary premolar extraction. Seventy-five drift models were obtained and digitalized via 3D scanning. The average physiologic drift duration was 81.66 ± 70.03 days. Angular and linear changes in the first molars, second premolars, and canines were measured using the 3D method. All the examined teeth had tipped and moved towards the extraction space, leading to space decreases. Posterior teeth primarily exhibited significant mesial tipping and displacement, without rotation or vertical changes. All canine variables changed, including distal inward rotation and extrusion. The physiologic drift tended to slow over time. Age had a limited negative effect on the mesial drift of posterior teeth, whereas crowding had a limited positive effect on canine drift. Thus, the mesial drift of molars after premolar extraction may lead to molar anchorage loss, particularly among younger patients. The pattern of the physiologic drift of maxillary canines can help relieve crowding and facilitate labially ectopic canine alignment, whereas canine drift is accelerated by more severe crowding.


Assuntos
Dente Pré-Molar/cirurgia , Imageamento Tridimensional , Extração Dentária , Migração de Dente , Adolescente , Adulto , Cefalometria , Criança , Feminino , Humanos , Masculino , Ortodontia , Análise de Regressão , Adulto Jovem
8.
Hum Fertil (Camb) ; : 1-8, 2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31607184

RESUMO

Endocrine and paracrine signals can be key regulators of ovarian physiology. The oocyte secretes growth factors which directly induce follicular development by a complex paracrine signalling process, and the transforming growth factorß (TGF-ß) superfamily has a pivotal role in this process. The bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) genes are relevant members of the TGF-ß superfamily that encode proteins secreted by the oocytes into the ovarian follicles, where they contribute to creating an environment supporting follicle selection and growth. Their main functions include regulating cellular proliferation/differentiation, follicular survival/atresia, and oocyte maturation. Recent functional studies have validated genetic factors (Progesterone receptor membrane component 1 (PGRMC1)), Fragile X mental retardation 1 (FMR1, GDF9 and BMP15) as being causative of primary ovarian insufficiency (POI), BMP15/GDF9 gene variants were found to have a high incidence on the POI phenotype. This review considers the most recent research regarding the role of BMP15 and GDF9 in the genetic control of follicular development, paying special attention to the pathogenesis of POI.

9.
Oncol Rep ; 42(4): 1343-1354, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31524236

RESUMO

Cisplatin is a common chemotherapeutic agent against ovarian cancer; however, drug resistance is a major limiting factor for its use in clinical treatment. The underlying mechanisms of cisplatin resistance in ovarian cancer have not yet been fully elucidated. Thus, this study aimed to elucidate some of the mechanisms responsible for resistance to cisplatin in ovarian cancer. The results demonstrated that the cisplatin­resistant human ovarian cancer cell lines, SKOV3/DDP and A2780/DDP, exhibited higher autophagy levels than the control ovarian cancer cell lines, SKOV3 and A2780. Moreover, autophagy inhibition by 3­methyladenine or shRNA against autophagy­related gene (ATG)5 potentiated the cytotoxicity induced by cisplatin, whereas autophagy induction by rapamycin (Rapa) increased cell survival. Exposure to cisplatin induced an upregulation in the expression of thioredoxin­related protein of 14 kDa (TRP14). Furthermore, TRP14 knockdown or overexpression decreased or increased the autophagy response and cisplatin resistance, and this effect was reversed by treatment with Rapa or ATG5 knockdown. The findings of this study also suggested that TRP14 induced autophagy and chemoresistance via the 5'AMP­activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR)/p70S6K signaling pathway. Importantly, the data from a tissue array revealed a positive association between TRP14 and Beclin1 in human ovarian cancer and marginal tissues. These findings have identified, for the first time, to the best of our knowledge, that TRP14 induces autophagy and consequently cisplatin resistance in ovarian cancer cells via the AMPK/mTOR/p70S6K signaling pathway. This in turn renders TRP14 as a potential predictor or target in ovarian cancer therapy.

10.
Korean J Orthod ; 49(4): 222-234, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31367577

RESUMO

Objective: To investigate the three-dimensional lip vermilion changes after extraction and non-extraction orthodontic treatment in female adult patients and explore the correlation between lip vermilion changes and incisor changes. Methods: Forty-seven young female adult patients were enrolled in this study (skeletal Class III patients were excluded), including 34 lip-protruding patients treated by extraction of four first premolars (18 patients requiring mini-implants for maximum anchorage control and 16 patients without mini-implants) and 13 patients requiring non-extraction treatment. Nine angles, seven distances, and the surface area of the lip vermilion were measured by using pre- and post-treatment three-dimensional facial scans. Linear and angular measurements of incisors were performed on lateral cephalograms. Results: There were no significant changes in the vermilion measurements in the non-extraction group. The vermilion angle, vermilion height, central bow angle, height/width ratio, and vermilion surface area decreased significantly after the orthodontic treatment in the extraction groups, but the upper/lower vermilion proportion remained unchanged. Significant correlations were found between the changes in incisor position and those in vermilion angles, vermilion height, and surface area. Conclusions: Extraction of the four first premolars probably produced an aesthetic improvement in lip vermilion morphology. However, the upper/lower vermilion proportion remained unchanged. The variations in the vermilion were closely related to incisor changes, especially the upper incisor inclination changes.

11.
Angle Orthod ; 89(5): 679-687, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-30920875

RESUMO

OBJECTIVES: To compare achieved and predicted tooth movements of maxillary first molars and central incisors in first premolar extraction cases treated with Invisalign. MATERIALS AND METHODS: The present study included 30 patients who received maxillary first premolar extraction treatment with Invisalign. The actual posttreatment model was registered with the pretreatment model on the palatal stable region and superimposed with the virtual posttreatment model. Achieved and predicted tooth movements of maxillary first molars and central incisors were compared using paired t-test. Linear mixed-effect model analyses were used to explore the influence of age (adolescents vs adults), attachment (G6-optimized vs 3-mm vertical, 3-mm horizontal, and 5-mm horizontal), and initial crowding on the differences between predicted and achieved tooth movement (DPATM). RESULTS: First molars achieved greater mesial tipping, mesial translation, and intrusion than predicted. Central incisors achieved less retraction and greater lingual crown torque and extrusion than predicted. Adolescents showed greater DPATM in the mesiodistal translation of first molars and labiolingual translation of central incisors and smaller DPATM in the occlusogingival translation of the first molars and crown torque of the central incisors than adults. The 3-mm vertical attachment group showed greater DPATM in the mesiodistal translation of the first molars vs the G6-optimized attachment group. Initial crowding had an inverse correlation with DPATM in angulation and mesiodistal translation of the first molars. CONCLUSIONS: First molar anchorage control and central incisor retraction were not fully achieved as predicted in first premolar extraction treatment with Invisalign. Age, attachment, and initial crowding affected the differences between predicted and achieved tooth movement.


Assuntos
Incisivo , Aparelhos Ortodônticos Removíveis , Técnicas de Movimentação Dentária , Adolescente , Adulto , Dente Pré-Molar , Humanos , Maxila , Dente Molar , Extração Dentária
12.
J Cell Physiol ; 234(8): 13894-13905, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-30656691

RESUMO

Vulvovaginal candidiasis (VVC) is a common observed infection, affecting approximately 75% of women of reproductive age. Drug resistance represents a troublesome stumbling block associated with VVC therapy. Thus the aim of the present study was to provide information regarding the selection of potential drug targets for VVC. CXCR3-, CXCR4-, or CXCR/CXCR4 double-deficient mouse models of VVC were subsequently established, with changes to the load of Candida Albicans evaluated accordingly. The biological behaviors of the vaginal epithelial cells were characterized in response to the CXCR3-, CXCR4-, or CXCR3/CXCR4 double-knockout in vivo. Our initial observations revealed that in mice with VVC, CXCR3-, CXCR4-, or CXCR3 - CXCR4 double-knockout resulted in a decreased load of C. Albicans as well as reduced levels and proportion of Th17 cells. Proinflammatory cytokine production was found to be inhibited by CXCR3-, CXCR4-, or CXCR3/CXCR4 double-knockout whereby the mRNA and protein expressions CXCR3, CXCR4, IL-17, IL-6, and TNF-α exhibited decreased levels. CXCR3-, CXCR4-, or CXCR3/CXCR4 double-knockout appeared to function as positive proliferation factors, while playing a negative role in the processes of apoptosis and the cell cycle of vaginal epithelial cells. Taken together, the key findings of the study suggested that CXCR3/CXCR4 double-knockout could act to hinder the progression of VVC, highlighting its promise as a novel therapeutic target in the treatment of VVC. CXCR3 and CXCR4 genes may regulate Th17/IL-17 immune inflammatory pathways to participate in antifungal immunity.


Assuntos
Candidíase Vulvovaginal/imunologia , Candidíase Vulvovaginal/metabolismo , Citocinas/biossíntese , Mediadores da Inflamação/metabolismo , Receptores CXCR3/deficiência , Receptores CXCR4/deficiência , Células Th17/patologia , Animais , Apoptose , Candida albicans/fisiologia , Candidíase Vulvovaginal/sangue , Candidíase Vulvovaginal/microbiologia , Ciclo Celular , Proliferação de Células , Citocinas/sangue , Células Epiteliais/metabolismo , Células Epiteliais/microbiologia , Células Epiteliais/patologia , Feminino , Camundongos Knockout , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Receptores CXCR3/sangue , Receptores CXCR3/metabolismo , Receptores CXCR4/sangue , Receptores CXCR4/metabolismo , Vagina/microbiologia , Vagina/patologia
13.
J Infect ; 77(3): 249-257, 2018 09.
Artigo em Inglês | MEDLINE | ID: mdl-29898409

RESUMO

Multiple reassortant strains of novel, highly pathogenic avian influenza A have recently emerged and spread over the world. Here we report on a 68-year-old woman in Jiangsu, China, with influenza A(H7N4) infection and associated illness, which strongly demonstrating the ability of the virus to spread from animals to humans and thus emphasizing the importance of continuous surveillance of the emerging viruses.


Assuntos
Vírus da Influenza A/classificação , Vírus da Influenza A/isolamento & purificação , Influenza Humana/diagnóstico , Influenza Humana/virologia , Idoso , Antibacterianos/administração & dosagem , Anti-Inflamatórios/administração & dosagem , Antivirais/administração & dosagem , China , Feminino , Genoma Viral , Humanos , Vírus da Influenza A/genética , Influenza Humana/patologia , Metilprednisolona/administração & dosagem , Moxifloxacina/administração & dosagem , Oseltamivir/administração & dosagem , Radiografia Torácica , Análise de Sequência de DNA , Tomografia Computadorizada por Raios X , Resultado do Tratamento
14.
PLoS One ; 13(5): e0197281, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29813088

RESUMO

OBJECTIVE: We compared tooth movement under maximum anchorage control with mini-screw implants in growing and non-growing patients. METHODS: In total, 15 adolescent (G1) and 19 adult (G2) patients with prognathic profiles were selected. All patients underwent first premolar extraction treatment with mini-screw implants for maximum anchorage control. Cone-beam computed tomography (CBCT) data were obtained immediately after implant placement (T1) and at the end of anterior tooth retraction (T2). Tooth movement and root length changes of the maxillary first molar, canine, and incisors were evaluated with three-dimensional models constructed using CBCT data obtained before and after orthodontic retraction through the superimposition of stable implants. RESULTS: Distal movement of the molar crown was observed in G2, but mesial movement was observed in G1. Mesial tipping of the first molar (1.82 ± 6.76°) was seen in G1 and distal tipping (4.44 ± 3.77°) was observed in G2. For the canines, mesial crown tipping (0.33 ± 4.99°) was noted in G1 and distal crown tipping (8.00 ± 5.57°) was observed in G2. In adults, the lingual inclinations of the lateral and central incisors were 11.91 ± 7.01° and 11.47 ± 6.70°, with 0.99 ± 1.22 mm and 1.08 ± 1.20 mm root retraction, respectively. In adolescents, the torque changes were smaller (lateral incisors, 8.25 ± 10.15°; central incisors, 9.82 ± 8.97°) and the root retractions were 0.31 ± 1.81 mm and 0.77 ± 1.59 mm, respectively. Less shortening of the central incisor roots occurred in adolescents than in adults. CONCLUSIONS: Tooth movements, such as anchor molar angular change, the canine tipping pattern, and the amount of incisor retraction, differed between adolescents and adults treated using the same anchorage with mini-screw implants, bracket prescription, and en masse retraction method. Anchorage strength of the first molars, canine movement patterns, and incisor retraction ranges are not determined by the anchorage device alone; growth and alveolar limitations also play roles.


Assuntos
Implantes Dentários , Técnicas de Movimentação Dentária , Adolescente , Criança , Tomografia Computadorizada de Feixe Cônico , Feminino , Humanos , Imageamento Tridimensional , Masculino , Procedimentos de Ancoragem Ortodôntica , Reabsorção da Raiz/diagnóstico por imagem , Reabsorção da Raiz/etiologia , Dente/diagnóstico por imagem , Técnicas de Movimentação Dentária/efeitos adversos , Adulto Jovem
15.
J Diabetes Investig ; 8(1): 34-43, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-27240324

RESUMO

AIMS/INTRODUCTION: To investigate the ability of human amniotic fluid stem cells (hAFSCs) to differentiate into insulin-producing cells. MATERIALS AND METHODS: hAFSCs were induced to differentiate into pancreatic cells by a multistep protocol. The expressions of pancreas-related genes and proteins, including pancreatic and duodenal homeobox-1, insulin, and glucose transporter 2, were detected by polymerase chain reaction and immunofluorescence. Insulin secreted from differentiated cells was tested by enzyme-linked immunosorbent assay. RESULTS: hAFSCs were successfully isolated from amniotic fluid that expressed the pluripotent markers of embryonic stem cells, such as Oct3/4, and mesenchymal stem cells, such as integrin ß-1 and ecto-5'-nucleotidase. Here, we first obtained the hAFSCs that expressed pluripotent marker stage-specific embryonic antigen 1. Real-time polymerase chain reaction analysis showed that pancreatic and duodenal homeobox-1, paired box gene 4 and paired box gene 6 were expressed in the early phase of induction, and then stably expressed in the differentiated cells. The pancreas-related genes, such as insulin, glucokinase, glucose transporter 2 and Nkx6.1, were expressed in the differentiated cells. Immunofluorescence showed that these differentiated cells co-expressed insulin, C-peptide, and pancreatic and duodenal homeobox-1. Insulin was released in response to glucose stimulation in a manner similar to that of adult human islets. CONCLUSIONS: The present study showed that hAFSCs, under selective culture conditions, could differentiate into islet-like insulin-producing cells, which might be used as a potential source for transplantation in patients with type 1 diabetes mellitus.


Assuntos
Líquido Amniótico/citologia , Diferenciação Celular , Células-Tronco Embrionárias/fisiologia , Células Secretoras de Insulina/fisiologia , Ilhotas Pancreáticas/fisiologia , Células-Tronco Mesenquimais/fisiologia , Adipogenia , Peptídeo C/metabolismo , Técnicas de Cultura de Células , Células Cultivadas , Células-Tronco Embrionárias/citologia , Humanos , Técnicas In Vitro , Células-Tronco Mesenquimais/citologia , Neurogênese
16.
Am J Orthod Dentofacial Orthop ; 150(1): 3-4, 2016 07.
Artigo em Inglês | MEDLINE | ID: mdl-27364194
17.
Int J Mol Med ; 37(5): 1310-6, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-27035617

RESUMO

Urokinase-type plasminogen activator (uPA) acts by breaking down the basement membrane and is involved in cell proliferation, migration and invasion. These actions are mediated by binding to the uPA receptor (uPAR) via its growth factor domain (GFD). The present study evaluated the effects of uPAg-KPI, a fusion protein of uPA-GFD and a kunitz protease inhibitor (KPI) domain that is present in the amyloid ß-protein precursor. Using SKOV-3 cells, an ovarian cancer cell line, we examined cell viability, migration, invasion and also protein expression. Furthermore, we examined wound healing, and migration and invasion using a Transwell assay. Our data showed that uPAg-KPI treatment reduced the viability of ovarian cancer SKOV-3 cells in both a concentration and time-dependent manner by arresting tumor cells at G1/G0 phase of the cell cycle. The IC50 of uPAg-KPI was 0.5 µg/µl after 48 h treatment. At this concentration, uPAg-KPI also inhibited tumor cell colony formation, wound closure, as well as cell migration and invasion capacity. At the protein level, western blot analysis demonstrated that uPAg-KPI exerted no significant effect on the expression of total extracellular signal-regulated kinase (ERK)1/ERK2 and AKT, whereas it suppressed levels of phosphorylated ERK1/ERK2 and AKT. Thus, we suggest that this novel uPAg-KPI fusion protein reduced cell viability, colony formation, wound healing and the invasive ability of human ovarian cancer SKOV-3 cells in vitro by regulating ERK and AKT signaling. Further studies using other cell lines will confirm these findings.


Assuntos
Aprotinina/metabolismo , Neoplasias Ovarianas/metabolismo , Proteínas Recombinantes de Fusão , Ativador de Plasminogênio Tipo Uroquinase/metabolismo , Aprotinina/genética , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Feminino , Humanos , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Neoplasias Ovarianas/patologia , Proteínas Proto-Oncogênicas c-akt/metabolismo , Proteínas Recombinantes de Fusão/farmacologia , Transdução de Sinais/efeitos dos fármacos , Ativador de Plasminogênio Tipo Uroquinase/genética
18.
Am J Orthod Dentofacial Orthop ; 149(3): 401-10, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26926028

RESUMO

This case report describes the treatment of a 25-year-old woman with a skeletal Class I pattern and moderate bimaxillary dentoalveolar protrusion. The orthodontic treatment included distal movement of her maxillary and mandibular dentitions using 1-stage miniscrews. The total active treatment time was about 12 months. Her tooth alignment and profile were significantly improved by the orthodontic treatment. The 2-year posttreatment records show a stable occlusion and satisfactory facial esthetics.


Assuntos
Parafusos Ósseos , Má Oclusão Classe I de Angle/terapia , Procedimentos de Ancoragem Ortodôntica/instrumentação , Técnicas de Movimentação Dentária/métodos , Adulto , Cefalometria/métodos , Tomografia Computadorizada de Feixe Cônico/métodos , Estética Dentária , Feminino , Seguimentos , Humanos , Incisivo/patologia , Mandíbula/patologia , Maxila/patologia , Miniaturização , Dente Molar/patologia , Técnicas de Movimentação Dentária/instrumentação , Resultado do Tratamento
19.
J Craniofac Surg ; 27(2): 405-9, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26872281

RESUMO

Sixty-nine experienced Chinese orthodontists evaluated 108 Chinese patients' facial attractiveness from set of photographs (frontal, lateral, and frontal smiling photos) taken at the end of orthodontic treatment. These 108 patients, which contained an equal number of patients with Class I, II, and III malocclusion, were randomly selected from 6 orthodontic treatment centers throughout China. Spearman rank-order correlation coefficients (rs) analyses were performed to examine agreement in ranking between all judge pairs. Pearson correlation and multivariate regression were performed to examine the correlation between cephalometric measures and end-of-treatment Photo Attractiveness Rank.96.68% judge pairs showed moderate correlated (+0.4 ≤ rs < +0.7) subjective rankings. Cephalometric measures significantly correlated with end-of-treatment Photo Attractiveness Rank included interincisal angle (r = 0.330, P < 0.05), L1/MP° (r = 0.386, P < 0.05), L1-NBmm (r = 0.451, P < 0.01), L1/NB° (r = 0.374, P < 0.05), and profile angle (r = 0.353, P < 0.05) in Class I patients with an explained variance of 32.8%, and ANB angle (r = 0.432, P < 0.01), angle of convexity (r = 0.448, P < 0.01), profile angle (r = 0.488, P < 0.01), Li to E-line (r = 0.374, P < 0.05), Li to B-line (r = 0.543, P < 0.01), and Z angle (r = 0.543, P < 0.01) in Class II patient with an explained variance of 43.3%.There was less association than expected between objective measurements on the lateral cephalograms and clinicians' rankings of facial attractiveness on clinical photography in Chinese patients. Straight-stand lower incisor was desired for facial attractiveness of Class I malocclusion; and sagittal relationship and lip prominence influence the esthetics of Class II malocclusion in Chinese population.


Assuntos
Cefalometria/métodos , Estética Dentária , Face/anatomia & histologia , Má Oclusão/terapia , Adolescente , Adulto , Criança , China , Feminino , Humanos , Masculino , Má Oclusão/diagnóstico , Sorriso , Adulto Jovem
20.
Beijing Da Xue Xue Bao Yi Xue Ban ; 47(6): 1000-4, 2015 Dec 18.
Artigo em Chinês | MEDLINE | ID: mdl-26679665

RESUMO

OBJECTIVE: To measure the resting pressure of the tongue body on the sagittal and vertical dimensions of the tongue anchorage pad (TAP), and to investigate the proper position of TAP as an anchorage. METHODS: Nineteen volunteers with individual normal occlusion (4 males and 15 females, age 23-33 years) were recruited in the study. Individualized TAP was designed and made for each subject. On the sagittal dimension, the pressure along the midline at the level of the distal of the second premolar (PM2), the first molar (M1) and the second molar (M2) were measured. On the vertical dimension, pressures on TAPs with height of -3 mm, 0 mm, and 3 mm were measured, with 0 mm standing for the reference point recorded by the tongue position record. The tongue resting pressure of the volunteers in the upright position was measured by miniature sensors (FSS1500NS, Honeywell, USA) embedded in TAP. Nonparametric analysis was applied with the significant level of 0.05. RESULTS: On the vertical dimension, the pressure obtained at the height of -3 mm, 0 mm, and 3 mm were 105.83 Pa, 167.75 Pa, and 254.25 Pa, respectively (P<0.001). On the sagittal dimension, the pressure detected at the level of PM2, M1, and M2 were 177.64 Pa, 126.72 Pa, and 109.37 Pa, respectively (P<0.001). CONCLUSION: Tongue pressure rises significantly with the increase of TAP height. On the sagittal dimension, pressure decreases along the palatal midline in an anteroposterior direction. But in the clinical practice, we should also put comfort into consideration.


Assuntos
Língua/fisiologia , Adulto , Dente Pré-Molar , Feminino , Humanos , Masculino , Dente Molar , Palato , Pressão , Dimensão Vertical , Adulto Jovem
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