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1.
Neuropsychologia ; : 107255, 2019 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-31715196

RESUMO

Episodic future thinking (EFT), the mental ability of projecting oneself into the future to pre-experience an event, has strong adaptive value for allowing people to consider potential consequences before taking actions. EFT includes two important components: the ability to construct detailed and vivid scenes of future events to achieve a goal (measured here by the EFT-sensory perceptual qualities scale) and the ability to subjectively experience "mental time travel" in which the person feels oneself to be in the future (measured here by the EFT-Autonoetic consciousness scale). However, little is known about the neuroanatomical structures of EFT. To shed light on this question, we employed voxel-based morphometry (VBM) to investigate the neural substrates underlying EFT. In Sample 1 (135 participants), EFT-sensory perceptual qualities was positively correlated with the gray matter (GM) volume of the hippocampus and putamen. EFT-Autonoetic consciousness was positively correlated with GM volume of the insula and amygdala, and negatively correlated with GM volumes of the medial frontal gyrus. The verification results from Sample 2 (59 participants) found that EFT-sensory perceptual qualities can be predicted by the GM volumes of the hippocampus and putamen, and EFT-Autonoetic consciousness can be predicted by the GM volumes of insula and amygdala. The present findings suggest that the hippocampus, putamen, and amygdala and insula are key regions for scene construction, goal-directed processing, and emotion respectively, and play important roles in the underlying structural neural substrates of EFT.

2.
Artigo em Inglês | MEDLINE | ID: mdl-31720816

RESUMO

PURPOSE: A randomized phase III trial demonstrated that gemcitabine plus cisplatin (GP) prolonged progression-free survival and overall survival compared with fluorouracil plus cisplatin (FP) as first-line chemotherapy in patients with metastatic nasopharyngeal carcinoma (NPC). The cost-effectiveness analysis was designed to identify the economic option for metastatic NPC from a Chinese societal perspective. METHODS: We established a Markov model that involved three health states representing the stages of disease to simulate therapy. Survival data of clinical outcomes were derived from the trial and adjusted to quality-adjusted life years (QALYs). Transition probabilities and health utilities were obtained from the clinical trial and published literatures. The cost-effective strategy was estimated for these treatments using a willing-to-pay (WTP) threshold. A one-way sensitivity analysis was conducted to study the influences of parameters. RESULTS: GP treatment group produced a gain of 0.37 QALYs with an incremental cost of $2520.80, yielding an incremental cost-effectiveness ratio (ICER) of $6812.97 per QALY, compared with FP treatment ($15,530.96 versus $13,010.16). The ICER was lower than the accepted WTP threshold, which was 3 times gross domestic product per capita of China ($25,749 per QALY). CONCLUSION: GP regimen is more cost-effective compared with FP regimen as the first-line treatment for Chinese patients with metastatic NPC.

3.
Pediatr Surg Int ; 2019 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-31701303

RESUMO

PURPOSE: To investigate the clinicopathololgic characteristics and the predicting value of preoperative imaging and tumor markers in children with ovarian masses. METHODS: Patients admitted in Shanghai children's hospital with ovary neoplasms between 2010.01 and 2015.12 were retrospectively analyzed. The medical records including age at operation, presentation of symptoms and signs, tumor marker, imaging, pathology, tumor diameter and surgical choice were reviewed. All data were analyzed using SPSS 17.0 RESULTS: A total of 139 patients were included, among which 116 were benign neoplasms and 23 malignant tumors. There was significance difference relation with the tumor diameter, character, torsion and tumor markers, but not the age, position, calculi, and symptoms. The risk factors include tumor diameters ≥ 10 cm, the odds ratio (OR) was 11, 95% confidence interval (CI) was 3-36, solid/complex tumor (OR 6, 95% CI 2, 14) and positive in tumor markers (OR 84, 95% CI 20, 345). Among the patients with benign neoplasms, 77 of them had laparoscopic ovarian cystectomy while 23 patients with malignant tumors had salpingo-oophorectomy and omentum resection. CONCLUSION: Preoperative imaging and tumor markers could help identifying the malignant ovarian masses in children. If tumor diameter ≥ 10 cm, solid/complex in imaging and tumor markers abnormal, a radical resection is mandatory; otherwise, an ovarian-sparing surgery is recommended.

4.
Chem Commun (Camb) ; 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31754670

RESUMO

A Lewis acid-catalyzed insertion of unsymmetrical alkynes into electron-deficient alkenes was developed for the first time, and used to produce 34 hitherto unreported pentacyclic benzo[5,6]chromeno[2,3-b]indoles with generally good yields and complete stereoselectivity. A Yb(OTf)3-catalyzed reaction between o-alkynylnaphthols and 3-methyleneindolin-2-ones proceeded efficiently, and provided a simple and convergent protocol for alkyne difunctionalization via oxidant-free C-C double bond breaking/rearrangement. Mechanistic details of this domino process were derived by conducting systematic theoretical calculations.

5.
J Asthma ; : 1-9, 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31738608

RESUMO

Objective: We sought to evaluate the accuracy of periostin for the diagnosis of asthma through a systematic review and meta-analysis.Methods: we searched the electronic databases (PubMed, EMBASE, Web of science) to find relevant studies through December 31, 2018. Studies selecting, data extraction and quality assessment were conducted by two researchers independently according to inclusion and exclusion criteria. We used bivariate mixed effects model to conduct the meta-analysis.Results: A total of 9 studies including 1448 subjects with asthma were analyzed. The pooled sensitivity, specificity, diagnostic odds ratio (DOR) were 0.68 (95% CI, 0.42-0.86), 0.87 (95% CI, 0.76-0.93) and 13.78 (95% CI, 4.64-40.93) respectively. The area under the summary receiver operating curves (SROC) was 0.87 (95% CI, 0.84-0.90). The Spearman correlation coefficient indicated that no threshold effect existed in the included studies. The study design had statistical significance for sensitivity results (p < 0.001) through regression analysis. Heterogeneity might be caused by non-threshold effect.Conclusion: Periostin has moderate accuracy in the diagnosis of asthma.

6.
Anal Chem ; 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31763820

RESUMO

Bacterial magnetic particles (BMPs) are an attractive carrier material for immunoassays because of its nanoscale size, dispersal ability, and membrane-bound structure. Anti-tetrabromobisphenol-A (TBBPA) nanobodies (Nbs) in the form of monovalence (Nb1), bivalence (Nb2) and trivalence (Nb3) were biotinylated and immobilized onto streptavidin (SA)-derivatized BMPs to construct the complex of BMP-SA-Biotin-Nb1, -Nb2 and -Nb3, respectively. An increasing order of binding capability of BMP-SA-Biotin-Nb1, -Nb2 and -Nb3 to TBBPA was observed. These complexes showed high resilience to temperature (90 °C), methanol (100%), high pH (12) and strong ionic strength (1.37 M NaCl). A BMP-SA-Biotin-Nb3-based enzyme linked immunosorbent assay (ELISA) for TBBPA dissolved in methanol was developed, showing a half-maximum inhibition concentration (IC50) of 0.42 ng mL-1. TBBPA residues in landfill leachate, sewage and sludge samples determined by this assay were in a range of

7.
J Gastrointest Surg ; 2019 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-31768829

RESUMO

A wide array of pathologic abnormalities can cause hepatic calcifications, which are relatively uncommon and may result from both benign and malignant tumors, as well as inflammatory and infectious conditions. A pattern recognition approach is important to follow when faced with characterization of a calcified hepatic lesion. Radiologists should be aware of morphologic imaging features of calcified liver lesions to help differentiate benign from malignant lesions. Liver biopsy should be offered when the diagnosis is doubtful.

8.
Org Lett ; 2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31769294

RESUMO

A novel photoredox catalysis for multiple functionalization of two different types of unactivated alkenes in a single operation was reported through a conceptually new mode of annulation-alkynyl migration. A wide array of cyclopentane carboxylates were synthesized in a highly selective and functional-group-compatible manner via C-centered radical induced cascade 5-exo-trig/5-exo-dig bicyclization and C-C bond cleavage process. This methodology might open a new entry for designing annulation-functional group migration to create structurally applicable cyclic ring systems.

9.
Mol Ther Nucleic Acids ; 18: 787-800, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31734559

RESUMO

Exosomes, carriers to transfer endogenous molecules, derived from bone marrow-derived mesenchymal stem cells (BMSCs) have been reported to play a role in the progression of bladder cancer. Here we aimed to test the functional mechanism of microRNA-9-3p (miR-9-3p)-containing exosomes derived from BMSCs in bladder cancer. BMSCs were cocultured with bladder cancer cells, and exosomes secreted from BMSCs were identified. Next, the expression of miR-9-3p and endothelial cell-specific molecule 1 (ESM1) in bladder cancer tissues and cells was determined. Then effects of miR-9-3p and ESM1 via BMSC-derived exosomes on bladder cancer cell viability, migration, invasion, and apoptosis were determined by loss- and gain-of-function experiments and on in vivo tumor growth, and metastasis was assessed in nude mice. miR-9-3p expression was decreased and ESM1 was increased in bladder cancer. BMSCs inhibited bladder cancer cell viability, migration, and invasion, and induced apoptosis, whereas the addition of exosome secretion inhibitor GW4869 achieved the opposite effects. Moreover, exosomal miR-9-3p upregulation or ESM1 silencing suppressed bladder cancer cell viability, migration, and invasion; induced cell apoptosis; and inhibited in vivo tumor growth and metastasis. Taken together, BMSC-derived exosomal miR-9-3p suppressed the progression of bladder cancer through ESM1 downregulation, offering a potential novel therapeutic target for bladder cancer therapy.

10.
ISME J ; 2019 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-31595051

RESUMO

Deep-sea hydrothermal vents and methane seeps are often densely populated by animals that host chemosynthetic symbiotic bacteria, but the molecular mechanisms of such host-symbiont relationship remain largely unclear. We characterized the symbiont genome of the seep-living siboglinid Paraescarpia echinospica and compared seven siboglinid-symbiont genomes. Our comparative analyses indicate that seep-living siboglinid endosymbionts have more virulence traits for establishing infections and modulating host-bacterium interaction than the vent-dwelling species, and have a high potential to resist environmental hazards. Metatranscriptome and metaproteome analyses of the Paraescarpia holobiont reveal that the symbiont is highly versatile in its energy use and efficient in carbon fixation. There is close cooperation within the holobiont in production and supply of nutrients, and the symbiont may be able to obtain nutrients from host cells using virulence factors. Moreover, the symbiont is speculated to have evolved strategies to mediate host protective immunity, resulting in weak expression of host innate immunity genes in the trophosome. Overall, our results reveal the interdependence of the tubeworm holobiont through mutual nutrient supply, a pathogen-type regulatory mechanism, and host-symbiont cooperation in energy utilization and nutrient production, which is a key adaptation allowing the tubeworm to thrive in deep-sea chemosynthetic environments.

11.
Artigo em Inglês | MEDLINE | ID: mdl-31617845

RESUMO

A Gram-stain-negative, non-motile, rod-shaped, oxidase-positive, red-pigmented bacterium, strain N3T, was isolated from Fuxian lake, a freshwater lake in Yunnan Province, PR China. Strain N3T was facultatively anaerobic, heterotrophic and negative for catalase. Optimal growth occurred at 30 °C (range 4-45 °C), pH 7.0-8.0 (range 6.5-9.5) and in the presence of 0-3 % (w/v) NaCl (range 0-3 %). The results of phylogenetic analysis based on 16S rRNA gene sequencing revealed that strain N3T was close to the type strains of Algoriphagus aquaeductus, Algoriphagus shivajiensis and Algoriphagus alkaliphilus with sequence similarities of 97.4, 97.3 and 97.2 % respectively. The G+C content of the genomic DNA was 43.9 mol%. The quinone system contained menaquinone MK-7 as the sole component. The major fatty acids were iso-C15 : 0, summed feature 9 (10-methyl C16 : 0 and/or iso-C17 : 1 ω9c), summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω 7c) and iso-C16 : 0. Major polar lipids were phosphatidylcholine, phosphatidylethanolamine, an unidentified glycolipid, one unidentified phospholipid, two unidentified aminolipids and four unidentified lipids. On the basis of physiological, chemotaxonomic and molecular properties as well as phylogenetic distinctiveness, strain N3T should be placed into the genus Algoriphagus as a novel species, for which the name Algoriphagus lacus sp. nov. is proposed. The type strain is N3T (=KCTC 62622T=MCCC 1H00308T).

12.
Curr Gene Ther ; 19(3): 172-196, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31566126

RESUMO

Long-term research on various types of RNAs has led to further understanding of diverse mechanisms, which eventually resulted in the rapid development of RNA-based therapeutics as powerful tools in clinical disease treatment. Some of the developing RNA drugs obey the antisense mechanisms including antisense oligonucleotides, small interfering RNAs, microRNAs, small activating RNAs, and ribozymes. These types of RNAs could be utilized to inhibit/activate gene expression or change splicing to provide functional proteins. In the meantime, some others based on different mechanisms like modified messenger RNAs could replace the dysfunctional endogenous genes to manage some genetic diseases, and aptamers with special three-dimensional structures could bind to specific targets in a high-affinity manner. In addition, the recent most popular CRISPR-Cas technology, consisting of a crucial single guide RNA, could edit DNA directly to generate therapeutic effects. The desired results from recent clinical trials indicated the great potential of RNA-based drugs in the treatment of various diseases, but further studies on improving delivery materials and RNA modifications are required for the novel RNA-based drugs to translate to the clinic. This review focused on the advances and clinical studies of current RNA-based therapeutics, analyzed their challenges and prospects.

13.
Adv Mater ; : e1903787, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31566259

RESUMO

Recent advances in nanofabrication technologies have spurred many breakthroughs in the field of photonic metamaterials that provide efficient ways of manipulating light-matter interaction at subwavelength scales. As one of the most important applications, photonic metamaterials can be used to implement novel optical absorbers. First the morphology engineering of various photonic metamaterial absorbers is discussed, which is highly associated with impendence matching conditions and resonance modes of the absorbers, thus directly determines their absorption efficiency, operational bandwidth, incident angle, and polarization dependence. Then, the recent achievements of various interdisciplinary applications based on photonic metamaterial absorbers, including structural color generation, ultrasensitive optical sensing, solar steam generation, and highly responsive photodetection, are reviewed. This report is expected to provide an overview and vision for the future development of photonic metamaterial absorbers and their applications in novel nanophotonic systems.

14.
Cereb Cortex ; 2019 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-31595961

RESUMO

The collection of eye gaze information during functional magnetic resonance imaging (fMRI) is important for monitoring variations in attention and task compliance, particularly for naturalistic viewing paradigms (e.g., movies). However, the complexity and setup requirements of current in-scanner eye tracking solutions can preclude many researchers from accessing such information. Predictive eye estimation regression (PEER) is a previously developed support vector regression-based method for retrospectively estimating eye gaze from the fMRI signal in the eye's orbit using a 1.5-min calibration scan. Here, we provide confirmatory validation of the PEER method's ability to infer eye gaze on a TR-by-TR basis during movie viewing, using simultaneously acquired eye tracking data in five individuals (median angular deviation < 2°). Then, we examine variations in the predictive validity of PEER models across individuals in a subset of data (n = 448) from the Child Mind Institute Healthy Brain Network Biobank, identifying head motion as a primary determinant. Finally, we accurately classify which of the two movies is being watched based on the predicted eye gaze patterns (area under the curve = 0.90 ± 0.02) and map the neural correlates of eye movements derived from PEER. PEER is a freely available and easy-to-use tool for determining eye fixations during naturalistic viewing.

15.
Respir Res ; 20(1): 223, 2019 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-31627757

RESUMO

BACKGROUND: Primary drug-resistant tuberculosis (DR-TB) has contributed to a significant health and economic burden on a global scale, especially in China. we sought to estimate epidemiological characteristics of primary DR-TB in China from 2004 to 2018. METHODS: Eleven thousand four hundred sixty-seven newly diagnosed and 1981 retreated TB cases with drug susceptibility data were included. Chi-Square test for trends, linear regression, a joinpoint regression model and temporal trend in proportions of the different resistance patterns were carried out. RESULTS: The proportion of primary DR-TB and mono-resistant TB (MR-TB) in China had reduced by more than 12% since 2004, and were 21.38%, 13.35% in 2018 respectively. Among primary DR-TB cases (2173,18.95%), the percentage of multiresistant TB (MDR-TB, from 5.41 to 17.46%), male (from 77.03 to 84.13%), cavity (from 13.51 to 43.92%), rifampicin(RFP)-resistant TB (from 8.11 to 26.98%), streptomycin(SM)-resistant TB (from 50.00 to 71.43%) increased significantly (P < 0.05). On the contrary, the proportion of female, non-cavity, isoniazide(INH)-resistant TB (from 55.41 to 48.15%) and MR-TB (from 82.43 to 62.43%) decreased significant (P < 0.05). The primary drug resistance rate among female, cavity, smoking, drinking, 15 to 44 year-old TB subgroups increased by 0.16, 6.24, 20.95, 158.85, 31.49%, respectively. The percentage of primary DR-TB, RFP-resistant TB dropped significantly during 2004-2007 in Joinpoint regression model. CONCLUSION: The total rate of drug resistance among new TB cases showed a downward trend in Shandong, China, from 2004 to 2018. Primary drug resistance patterns were shifting from female, non-cavity, INH-resistant TB, and MR-TB groups to male, cavity, RFP/SM-resistant TB, and MDR-TB groups. Considering the rising drug resistance rate among some special population, future control of primary DR-TB in China may require an increased focus on female, cavity, smoking, drinking, or 15 to 44 year-old TB subgroups.

16.
Sensors (Basel) ; 19(21)2019 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-31652974

RESUMO

Underwater acoustic technology is an important means of detecting the ocean. Due to the complex influence of the marine environment, there is a lot of noise and baseline drift in the signals collected by hydrophones. In order to solve this problem, this paper proposes a denoising and baseline drift removal algorithm for MEMS vector hydrophone based on whale-optimized variational mode decomposition (VMD) and correlation coefficient (CC). Firstly, the power spectrum entropy (PSE), which reflects the variation characteristics of the signal frequency is selected as the fitness function of the whale-optimization algorithm to find the parameters (K,α) of the VMD. It is easier to find the global optimal solution of the parameters by combining the whale-optimization algorithm. Then, using the VMD algorithm after obtaining the parameters, the original signal is decomposed to obtain the intrinsic mode functions (IMFs), and calculating the correlation coefficients (CCs) between the IMFs and the original signal. Finally, the CC threshold is used to remove the noise IMFs, and the rest of the useful IMFs are reconstructed to complete the denoising and baseline drift removal process of the original signals. In the simulation experiments, the algorithm proposed in this paper shows better performance by comparing conventional digital signal-processing methods and the related algorithms proposed recently. Applied in the experiments of a MEMS hydrophone, the effectiveness of the proposed algorithm is also verified. This algorithm can provide new ideas for signal denoising and baseline drift removal.

17.
Biol Lett ; 15(10): 20190504, 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31640530

RESUMO

Pectinodontid limpets are important members of deep-sea hot vents and cold seeps as can be seen by their conspicuous presence in both extant and extinct systems. They have traditionally been classified into different genera and species based on shell and radula characteristics; the reliability of these characters has been questioned but not tested thoroughly. Here, for the first time in taxa endemic to deep-sea chemosynthetic ecosystems, we combine substrate translocation with molecular data to assess the plasticity and variability of key phenotypic characters. Molecular data revealed that several 'species' of extant vent/seep pectinodontids actually represent intergrading morphotypes of a single, highly plastic, evolutionary lineage, with each morphological trait being possibly influenced differently by environmental and genetic factors. Our results challenge previous interpretations of paleoecology at fossil chemosynthetic ecosystems and highlight the importance of modern analogues in understanding fossil systems.

18.
Bioengineered ; 10(1): 459-468, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31601152

RESUMO

Biomarkers for predicting chemotherapy response are important for treatment of colorectal cancer (CRC) patients.SREBP1is involved in cancer cell chemoresistance, but the biological consequences of this activity in CRC are poorly understood. We set up biochemical and cell biology analyzes to analyze SREBP1 expression and chemoresistance. We found that SREBP1 was overexpressed in chemoresistant CRC samples, and that SREBP1 overexpression was correlated with poorer patient survival. Targeting SREBP1 increased chemosensitivity to gemcitabine (Gem) in CRC cells. Additionally, SREBP1 overexpression increased chemoresistance to Gem in CRC cells. SREBP1 overexpression downregulated caspase-7 and decreased CRC cell sensitivity to Gem. Low SREBP1 expression was correlated with high caspase-7 expression in CRC patient samples. Low caspase-7 expression was correlated with poor patient survival. Our findings indicated that upregulation of caspase-7 caused by downregulation of SREBP1 may be a novel prognostic biomarker, and may represent a new therapeutic target in CRC.

19.
Artigo em Inglês | MEDLINE | ID: mdl-31647444

RESUMO

To reveal how gene regulatory networks change over cancer development, multiple time-varying differential networks between adjacent cancer stages should be estimated simultaneously. Since the network rewiring may be driven by the perturbation of certain individual genes, there may be some hub nodes shared by these differential networks. Although several methods have been developed to estimate differential networks from gene expression data, most of them are designed for estimating a single differential network, which neglect the similarities between different differential networks. In this study, we propose a new Gaussian graphical model-based method to jointly estimate multiple time-varying differential networks for identifying network rewiring over cancer development. A D-trace loss is used to determine the differential networks. A tree-structured group Lasso penalty is designed to identify the common hub nodes shared by different differential networks and the specific hub nodes unique to individual differential networks. Simulation experiment results demonstrate that our method outperforms other state-of-the-art methods. We also apply our method to The Cancer Genome Atlas data to explore gene network rewiring over different breast cancer stages. Hub nodes in the estimated differential networks rediscover well known genes associated with the development and progression of breast cancer.

20.
Fish Shellfish Immunol ; 94: 327-335, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31491528

RESUMO

The lamprey is a primitive jawless vertebrate that occupies a critical phylogenetic position, and its larval stage represents the major portion of its life cycle [1]. Lamprey larvae have been proven to be an important model organism for studying numerous biological problems, such as the immune system, due to their unique biological features [2]. In addition, early-stage larvae have never been obtained from the wild [3]; therefore, it is necessary to establish artificial breeding of lampreys in the laboratory. However, during early development, the larvae exhibit susceptibility to saprolegniasis, and the immune responses of lamprey larvae to this infection remain poorly understood. Here, we established a model of fungal infection in lamprey larvae and then used RNA sequencing to investigate the transcript profiles of lamprey larvae and their immune responses to Saprolegnia ferax. Among the profiled molecules, genes involved in pathogen recognition, inflammation, phagocytosis, lysosomal degradation, soluble humoral effectors, and lymphocyte development were significantly upregulated. The results were validated by analysis of several genes by quantitative real-time PCR and whole-mount in situ hybridization. Finally, we performed a Western blot for VLRs in infected and uninfected lampreys. This work not only provides an animal model for studying fungal infection but also suggests a molecular basis for developing defensive strategies to manage Saprolegnia ferax infection.

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