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1.
Zhonghua Zhong Liu Za Zhi ; 41(8): 633-637, 2019 Aug 23.
Artigo em Chinês | MEDLINE | ID: mdl-31434457

RESUMO

Objective: To investigate whether elevated levels of C-reactive protein (CRP) and neutrophil (NE) in the blood is associated with an increased risk of lung cancer incidence. Methods: From 2006 to 2007, all employees and retirees from Kailuan (Group) Limited liability Corporation were included in this Kailuan Cohort study. The last follow-up date was December 2015. Data on new cases of lung cancer were collected, and multivariable Cox proportional hazards regression models were used to the relationship between baseline CRP and NE at baseline and risk of lung cancer. Results: A total of 92 735 participants were enrolled in this study. During the follow-up, 850 new cases of lung cancer were identified. All subjects were divided into four groups according to the combination level of CRP and NE at baseline: CRP≤3 mg/L and NE≤4×10(9)/L(Group A), CRP≤3 mg/L and NE>4×10(9)/L(Group B), CRP>3 mg/L and NE≤4×10(9)/L(Group C), CRP>3 mg/L and NE>4×10(9)/L(Group D). The cumulative incidence of lung cancer were 950/100 000, 1 030/100 000, 1 081/100 000 and 1 596/100 000 in these four groups, respectively (P<0.001). Multivariate Cox proportional risk model showed that participants from Group D had an significantly increased 72% risks of lung cancer when compared to Group A (95% CI: 1.40~2.12, P<0.001). Stratified analyses gender showed that males in Group D had higher risk of lung cancer when compared with participants in Group A (HR=1.73, 95% CI: 1.40~2.15, P<0.001). Conclusion: Elevated levels of CRP and NE might increase the risk of lung cancer.


Assuntos
Neoplasias Pulmonares/epidemiologia , Proteína C-Reativa/metabolismo , Feminino , Humanos , Contagem de Leucócitos , Neoplasias Pulmonares/sangue , Masculino , Neutrófilos , Modelos de Riscos Proporcionais , Estudos Prospectivos , Fatores de Risco
2.
Zhonghua Yi Xue Za Zhi ; 97(40): 3158-3161, 2017 Oct 31.
Artigo em Chinês | MEDLINE | ID: mdl-29081162

RESUMO

Objective: To investigate whether elevated levels of high sensitivity C-Reactive Protein (hsCRP) and neutrophil (NE) at baseline are associated with an increased risk of colorectal cancer in Kailuan male cohort. Methods: Since May 2006, males from Kailuan cohort were included in this study. Information on demographics, medical history, anthropometry, hsCRP and NE were collectedat baseline for all subjects. Multivariable Cox proportional hazards regression models were used to calculate hazard ratios (HR) of association between baseline hsCRP and NE and colorectal cancer risk. Results: By December 31, 2015, a total of 73 869 participants were enrolled in this study. During the follow-up, 336 incident colorectal cancer cases were identified. All participants were divided into three groups according to the level of hsCRP (<1 mg/L, 1-3 mg/L and >3 mg/L). The cumulative incidence of colorectal cancer were 456/10(5,) 510/10(5) and 746/10(5) in these 3 groups, respectively (χ(2)=10.79, P=0.005). Compared with participants with lower hsCRP levels (<1 mg/L), individuals with the highest hsCRP (>3 mg/L) levels had significant increased risks of colorectal cancer (HR=1.38, 95%CI: 1.05-1.81, P=0.020)after adjusting for age, gender, smoking, drinking, BMI, diabetes and income. Furthermore, subjects were divided into two groups according to the level of NE (≤ 4.08×10(9)/L and > 4.08×10(9)/L). Multivariable Cox proportional hazards regression models indicated that there is no statistical significance of association between NE and colorectal cancer. Conclusions: Elevated levels of hsCRP at baseline might increase the risk of colorectal cancer in males.


Assuntos
Biomarcadores Tumorais/análise , Proteína C-Reativa/análise , Neoplasias Colorretais/diagnóstico , Neoplasias do Colo , Humanos , Incidência , Masculino , Modelos de Riscos Proporcionais , Estudos Prospectivos , Fatores de Risco
3.
Undersea Hyperb Med ; 42(5): 383-8, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26591977

RESUMO

We are living in an environment full of gases, and any change in the concentration of a component of the air or contaminants (usually toxic) in the air may significantly threaten human health. Thus, to investigate the influence of gases in animal models it is helpful to elucidate the pathogenesis of gas-related injury. Although there are devices used for gas exposure in animals, there are still limitations in the establishment of these animal models, such as the change in gas concentration during the refreshing of water, food and litter, and the contamination of toxic gases released by animals. Herein, we freshly prepared a chamber for normobaric gas exposure. During the exposure in this chamber, the refreshing of water, food and litter does not require opening of the chamber. The chamber gases are continuously circulated and filtered, and the gas concentration remains very stable. To validate the feasibility of this chamber, rats were exposed to pure oxygen as an example. Results showed that rats with hyperoxia-induced lung injury simulated by pure oxygen exposure displayed the representative characteristics as observed in humans: shortness of breath, lung edema, alveolar septal rupture, infiltration of inflammatory cells, oxidative and inflammatory injury. This suggests that it is feasible to establish animal models using this chamber for the investigation of gas toxicity.


Assuntos
Câmaras de Exposição Atmosférica , Modelos Animais de Doenças , Hiperóxia/complicações , Lesão Pulmonar/complicações , Oxigênio , Amônia/análise , Animais , Dióxido de Carbono/análise , Monóxido de Carbono/análise , Dispneia/etiologia , Ambiente Controlado , Desenho de Equipamento , Estudos de Viabilidade , Glutationa/análise , Sulfeto de Hidrogênio/análise , Lesão Pulmonar/induzido quimicamente , Malondialdeído/análise , Estresse Oxidativo , Alvéolos Pulmonares/lesões , Edema Pulmonar/etiologia , Ratos , Ruptura/etiologia
4.
Br J Radiol ; 88(1051): 20140590, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25939819

RESUMO

OBJECTIVE: To compare the differences between contrast-enhanced (CE) fluorine-18 fludeoxyglucose ((18)F-FDG) positron emission tomography (PET)/CT and CECT in target volume delineation and radiotherapy (RT) dose distribution, and to evaluate the sparing of organs at risk (OARs) in the treatment plan of locally advanced pancreatic cancer (LAPC). METHODS: 21 consecutive patients with LAPC with histologically or cytologically confirmed adenocarcinoma underwent both non-CECT and (18)F-FDG scans; 11 of whom also underwent CECT scans. Intensity-modulated RT plans (prescribed dose, 54 Gy) were constructed to cover the corresponding gross tumour volume (GTV). The differences among GTVCT, GTVPET, GTVPET-CT and OARs in these different image sets as well as the uniformity of target dose were analysed. RESULTS: The mean non-CE GTVCT, GTVPET and GTVPET-CT were 76.9 ± 47.8, 47.0 ± 40.2 and 44.5 ± 34.7 cm(3) (mean ± standard deviation), respectively. The non-CE GTVPET-CT was significantly smaller than the non-CE GTVCT (p < 0.001). The CE GTVPET-CT was significantly smaller than the CE GTVCT (p = 0.033). For both the non-CE GTVCT and the CE GTVCT, the intestine V40 (the percentage of the intestine volume irradiated by 40 Gy), intestine V50, intestine Dmax (the mean maximum dose), cord Dmax, left kidney V30, right kidney V30, left kidney Dmean (the mean dose), right kidney Dmean and liver V30 were 5.90%, 2.52%, 5500 cGy, 2194 cGy, 3.40%, 0.68%, 747 cGy, 550 cGy and 5.37%, respectively. There are significant differences between the non-CE CT and the non-CE PET-CT in intestine Dmax (p = 0.023) and right kidney Dmean (p = 0.029). CONCLUSION: Co-registration of (18)F-FDG PET with CECT may improve the accuracy of GTV delineation in LAPC and might reduce the adverse effect of irradiation. ADVANCES IN KNOWLEDGE: Individual adaptation of RT based on functional CE (18)F-FDG PET/CT imaging is possible and highly promising in LAPC.


Assuntos
Adenocarcinoma/radioterapia , Fluordesoxiglucose F18 , Neoplasias Pancreáticas/radioterapia , Tomografia por Emissão de Pósitrons/métodos , Compostos Radiofarmacêuticos , Tomografia Computadorizada por Raios X/métodos , Adenocarcinoma/patologia , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica , Órgãos em Risco , Neoplasias Pancreáticas/patologia , Neoplasias Peritoneais/secundário , Dosagem Radioterapêutica , Planejamento da Radioterapia Assistida por Computador , Carga Tumoral
5.
Eur J Surg Oncol ; 41(4): 527-33, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25690648

RESUMO

OBJECTIVE: Use of ultrasonic surgical instrument is gaining popularity for dissection and coagulation in open surgery. However, there is still no consensus on the efficacy and safety of its use compared with conventional surgical technique in open gastrectomy for gastric cancer. The aim of this meta-analysis was to evaluate the role and surgical outcomes of ultrasonic dissection (UD) compared with conventional electrocautery (EC). METHODS: A systematic literature search was performed to identify all studies comparing UD and EC in gastric cancer surgery. Intraoperative and postoperative outcomes were compared using weighted mean differences (WMDs) and odds ratios (ORs). RESULTS: Five studies were included in this meta-analysis, comprising 489 patients. Meta-analysis results showed that compared with EC, UD was associated with significantly shorter operation time (P = 0.03), less intraoperative blood loss (P = 0.002), lower morbidity (P = 0.02), and reduced postoperative hospital stay (P = 0.03). However, there was no significant difference between the two surgical techniques with regards to postoperative abdominal drainage (P = 0.17), and total cost in hospital (P = 0.59). CONCLUSIONS: Compared to EC, the use of UD during open gastrectomy can provide several improved outcomes for operation time, intraoperative blood loss, overall morbidity, and postoperative hospital stay. It appears that UD can be used instead of conventional EC in open gastric cancer surgery, although more larger trials with long follow-up should be performed.


Assuntos
Dissecação/métodos , Eletrocoagulação , Gastrectomia/métodos , Neoplasias Gástricas/cirurgia , Procedimentos Cirúrgicos Ultrassônicos , Perda Sanguínea Cirúrgica , Dissecação/efeitos adversos , Eletrocoagulação/efeitos adversos , Gastrectomia/efeitos adversos , Humanos , Tempo de Internação , Duração da Cirurgia , Ensaios Clínicos Controlados Aleatórios como Assunto , Procedimentos Cirúrgicos Ultrassônicos/efeitos adversos
6.
Undersea Hyperb Med ; 41(1): 27-31, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24649714

RESUMO

Iron is essential to life due to its unusual flexibility in serving as both an electron donor and acceptor. However, free iron can damage tissues by catalyzing the conversion of hydrogen peroxide to free-radical ions that attack lipids, proteins and DNA. Hyperoxia-induced lung injury (HILI) occurs when breathing elevated partial pressure of oxygen (usually > 0.5 atmospheres absolute) for extended periods. A few studies have shown that iron and proteins related to iron metabolism are closely related to HILI, and iron chelation may exert protective effects on HILI. As a rate-limiting enzyme in the degradation of heme, heme oxygenases (HOs) play a crucial role in the iron metabolism. Although some studies have been conducted to investigate the role of HOs in the pathogenesis of HILI, findings still conflict, and HOs of different isoforms may function differently in the pathogenesis of HILI. On the available findings, there might be a beneficial threshold of HO-1 expression in HILI. More studies are required to confirm the above findings and to provide evidence for the clinical treatment of HILI by iron chelation.


Assuntos
Heme Oxigenase (Desciclizante)/metabolismo , Hiperóxia/complicações , Ferro/efeitos adversos , Lesão Pulmonar/etiologia , Animais , Humanos , Hiperóxia/metabolismo , Ferro/metabolismo , Quelantes de Ferro/administração & dosagem , Lesão Pulmonar/metabolismo , Lesão Pulmonar/prevenção & controle , Ratos
7.
Osteoarthritis Cartilage ; 21(11): 1700-6, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23942062

RESUMO

OBJECTIVE: Knee osteoarthritis (KOA) is a common disease that is characterized by the degeneration of joint cartilage in the knee. Genetic factors have been implicated in KOA. Recently, several genetic studies have suggested that susceptibility to KOA is affected by the number of aspartic acid (D) residues in the amino-terminal of the asporin protein, but evidence remains conflicting. Therefore, the objective of the present meta-analysis was to investigate whether or not the D-repeat polymorphism is associated with susceptibility to KOA. METHODS: A systematic search of all relevant studies published through Dec 2012 was conducted using the MEDLINE, EMBASE, OVID, and ScienceDirect. Allelic counts were evaluated for the D14 and D13 alleles respectively. The included studies were only assessed in the analysis of the following allele model: D14 allele vs others alleles combined, D13 allele vs others alleles combined, and D14 allele vs D13 allele. RESULTS: Seven studies (eight comparisons) with 5515 total participants (2334 KOA patients and 3181 controls), which involved four Caucasian and four Asian populations, were eligible for inclusion. Meta-analysis was conducted for genotype D14 vs others combined, D13 vs others combined, and D14 vs D13. In the stratification based on ethnicity, studies were divided into Caucasian and Asian populations. We did not detect positive association between KOA and the D14 allele in Asian populations (OR = 1.527, 95% CI: 0.879-2.653) and in Caucasian populations (OR = 1.053, 95% CI: 0.905-1.225). There was also no positive association between susceptibility to KOA and D13 allele in Asian populations (OR = 0.950, 95% CI: 0.732-1.233) and in Caucasian populations (OR = 0.866, 95% CI: 0.723-1.037). CONCLUSION: The present results suggest that the D-repeat of asporin gene (ASPN) may not be a major susceptibility locus in the Caucasian and Asian populations with KOA. Because of the limitations of the present meta-analysis, accurate conclusions could not be drawn based on the current evidence, and further studies with large sample size are required.


Assuntos
Ácido Aspártico/genética , Proteínas da Matriz Extracelular/genética , Osteoartrite do Joelho/genética , Polimorfismo Genético , Grupo com Ancestrais do Continente Asiático/genética , Grupo com Ancestrais do Continente Europeu/genética , Frequência do Gene , Predisposição Genética para Doença , Humanos , Osteoartrite do Joelho/etnologia
8.
Int J Immunopathol Pharmacol ; 26(2): 371-81, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23755752

RESUMO

Asthma is an inflammatory disease of the airways, and the current treatment in managing asthma is the control of inflammation. Notch signaling pathway has been linked to T-cell imbalance. The present study aimed to explore the histone modifications of Notch1 promoter in normal and asthmatic lung CD4+ T cells. Chromatin immunoprecipitation analysis showed that the acetylation levels of total H3, H4, site-specific H3K9, H3K14, H3K27, H3K18, H4K16, and the trimethylation levels of H3K4, H3K79 of Notch1 gene promoter were increased significantly in asthmatic lung CD4+ T cells compared to the control group, which correlated with increased P300, PCAF activity and decreased HDAC1, HDAC2 activity. After intervention of garcinol, a potent inhibitor of histone acetyltransferases, in asthmatic lung CD4+ T cells, HAT activity decreased significantly and the increased Notch1 and hes-1 expression was reversed. The total H3ac, H4ac, site-specific H3K9ac, H3K14ac, H3K27ac, H3K18ac, H4K16ac and H3K79me3 levels of Notch1 gene promoter decreased significantly, and the H3K4me3, H3K9me3, H4K20me3 levels had no significant difference. We further investigated the suppressive effects of GAR on asthmatic parameters. Results showed that the levels of IL-4, IL-5 and IL-13 were significantly reduced and a small reverse trend was found in the level of IFN-g after GAR treatment. Furthermore, the expression of NF-κB and AP-1 reduced significantly. These results suggest that asthma is associated with changes in the epigenetic status of Notch1 promoter, including abnormal histone acetylation and methylation, and GAR may have applications in the treatment of asthma.


Assuntos
Asma/metabolismo , Linfócitos T CD4-Positivos/metabolismo , Diferenciação Celular , Histonas/metabolismo , Pulmão/metabolismo , Regiões Promotoras Genéticas , Receptor Notch1/genética , Acetilação , Animais , Antiasmáticos/farmacologia , Asma/induzido quimicamente , Asma/genética , Asma/imunologia , Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Linfócitos T CD4-Positivos/efeitos dos fármacos , Linfócitos T CD4-Positivos/imunologia , Diferenciação Celular/efeitos dos fármacos , Células Cultivadas , Citocinas/metabolismo , Modelos Animais de Doenças , Epigênese Genética , Histona Desacetilase 1/metabolismo , Histona Desacetilase 2/metabolismo , Inibidores de Histona Desacetilases/farmacologia , Proteínas de Homeodomínio/metabolismo , Mediadores da Inflamação/metabolismo , Pulmão/efeitos dos fármacos , Pulmão/imunologia , Masculino , Metilação , NF-kappa B/metabolismo , Ovalbumina , Regiões Promotoras Genéticas/efeitos dos fármacos , Ratos , Ratos Wistar , Transdução de Sinais , Terpenos/farmacologia , Fator de Transcrição AP-1/metabolismo , Fatores de Transcrição HES-1 , Fatores de Transcrição de p300-CBP/metabolismo
11.
Dis Esophagus ; 25(7): 638-44, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22236447

RESUMO

In the light of increasing evidence supporting cancer stem cells (CSCs) theory, the expression of two stem cell markers, CD133 and adenosine triphosphate-binding cassette superfamily G member 2 (ABCG2), in esophageal squamous cell carcinoma (ESCC) was investigated, and their prognostic values were evaluated. Paraffin-embedded tissue sections of 110 ESCC patients were investigated using Immunohistochemistry. The association of CD133 and ABCG2 expression with clinicopathologic characteristics was analyzed by χ(2) test. Survival analysis was carried out using Kaplan-Meier method and Cox proportional hazards model. CD133 and ABCG2 expression were detected in 27.3% and 15.5% of ESCC patients, respectively. The presence of CD133-positive cancer cells was associated with tumor cell differentiation (P= 0.008) but not significantly related to the survival of ESCC patients (P= 0.085). ABCG2 expression was not associated with clinicopathologic characteristics but was a significant prognostic factor for adverse overall survival of ESCC patients (P= 0.005). The median overall survival time for ESCC patients with and without ABCG2 expression were 21.8 and >49.3 months, respectively. A combined analysis of CD133 and ABCG2 expression did not show that ESCC patients with coexpression of these two markers had a worse prognosis than those with only ABCG2 expression (P= 0.934). Moreover, ABCG2 expression was revealed to be an independent prognostic factor along with tumor node metastasis stage in multivariate analysis (hazard ratio of ABCG2, 3.38; 95% confidence interval, 1.61∼7.09; P= 0.001). By survival analysis based on tumor node metastasis stage of ESCC, the association between ABCG2 expression and the patients' prognosis was found significant in the group of relatively early stage (P= 0.005) and marginally significant in the group of relatively late stage (P= 0.058). This is the first time to report the presence of CD133-positive cancer cells in ESCC but not supporting its prognostic value and validity as a CSC marker for ESCC. ABCG2 expression was found to correlate with the survival of ESCC patients, especially those at relatively early stage, suggesting that ABCG2-positive cancer cells may represent a pool of CSCs in ESCC, and relatively early-stage patients with ABCG2 expression may deserve more intensive or targeted therapy.


Assuntos
Transportadores de Cassetes de Ligação de ATP/metabolismo , Antígenos CD/metabolismo , Carcinoma de Células Escamosas/diagnóstico , Neoplasias Esofágicas/diagnóstico , Glicoproteínas/metabolismo , Proteínas de Neoplasias/metabolismo , Células-Tronco Neoplásicas/metabolismo , Peptídeos/metabolismo , Antígeno AC133 , Membro 2 da Subfamília G de Transportadores de Cassetes de Ligação de ATP , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma de Células Escamosas/metabolismo , Neoplasias Esofágicas/metabolismo , Carcinoma de Células Escamosas do Esôfago , Feminino , Humanos , Imuno-Histoquímica , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Prognóstico , Estudos Retrospectivos
12.
Eur J Clin Microbiol Infect Dis ; 31(6): 1233-42, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22042558

RESUMO

Multidrug-resistant tuberculosis (MDR-TB) has become a lethal global threat. Insights into the immune regulation of MDR-TB are urgently needed for the development of new treatments; however, the T cell response to an MDR-TB infection in human remains unclear. In the present study, the proportion of Th1 and Th2 cell subsets and the level of related T cell subset cytokines in peripheral blood were investigated. We detected that an MDR-TB infection resulted in suppressed Th1 and Th2 cell activation, which was more remarkable in patients with MDR-TB than that in drug-sensitive tuberculosis (DS-TB) sufferers when compared to healthy controls (HCs). In addition, MDR-TB infection down-regulated the expression of IFN-γ, IL-2, and IL-10, and up-regulated IL-4, IL-6, and TNF-α expression. Our data suggest that the disturbance between protective and pathogenic effects induced by the immunosuppression of Th1- and Th2-type responses is a substantial characteristic of MDR-TB infections.


Assuntos
Células Th1/imunologia , Células Th2/imunologia , Tuberculose Resistente a Múltiplos Medicamentos/imunologia , Adolescente , Adulto , Idoso , Sangue/imunologia , Citocinas/metabolismo , Feminino , Humanos , Tolerância Imunológica , Masculino , Pessoa de Meia-Idade , Subpopulações de Linfócitos T/imunologia , Adulto Jovem
13.
Int J Immunopathol Pharmacol ; 25(4): 989-1001, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23298489

RESUMO

Yes-associated protein 1 (YAP1), a nuclear effector of the Hippo pathway, plays an important role in tumorigenesis and progression of multiple cancers. The present study aimed to investigate the clinical significance of YAP1 and receptor tyrosine kinase Axl expression in human lung adenocarcinomas (LAC). We further explored possible molecular mechanisms mediated by YAP1 in LAC and gastric adenocarcinoma (GAC) cells. Forty-nine cases of human LAC and normal lung tissue (NLT) were collected. The expression of YAP1 and Axl was assessed by immunohistochemical assay through tissue microarray procedure and the clinicopathologic characteristics of all patients were analyzed. Using a loss of function approach, we investigated the effects of small hairpin RNA (shRNA)-mediated knockdown of YAP1 on the expression of Axl, proliferating cell nuclear antigen (PCNA) and matrix metalloproteinase-9 (MMP-9), and the proliferative activities and invasive potential in LAC A549 and GAC SGC-7901 cell lines. As a result, the expression of YAP1 and Axl was found in LAC tissues with higher strong reactivity rate compared to the NLT (87.8 percent vs.60.8 percent, P=0.000; 77.6 percent vs 0.0 percent, P=0.000), but they did not associate with the age, gender, tumor size, TNM staging or lymph node metastases of LAC patients (each P>0.05). Spearman rank correlation analysis showed a positive correlation between YAP1 and Axl expression. Furthermore, knockdown of YAP in vitro markedly down-regulated the expression of Axl, PCNA and MMP-9, and inhibited the proliferation and invasion of LAC and GAC cells. Taken together, YAP1 and Axl are highly expressed in LAC compared to the NLT, and knockdown of YAP1 may inhibit the proliferation and invasion of adenocarcinoma cells through downregulation of the Axl pathway, representing a potential therapeutic target for the treatment of cancer.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/fisiologia , Adenocarcinoma/patologia , Neoplasias Pulmonares/patologia , Fosfoproteínas/fisiologia , Proteínas Proto-Oncogênicas/fisiologia , Receptores Proteína Tirosina Quinases/fisiologia , Proteínas Adaptadoras de Transdução de Sinal/análise , Adenocarcinoma de Pulmão , Adulto , Idoso , Linhagem Celular Tumoral , Proliferação de Células , Ativação Enzimática , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica , Metástase Neoplásica , Fosfoproteínas/análise , Antígeno Nuclear de Célula em Proliferação/análise , Proteínas Proto-Oncogênicas/análise , Receptores Proteína Tirosina Quinases/análise , Fatores de Transcrição
14.
J Colloid Interface Sci ; 351(1): 210-6, 2010 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-20709329

RESUMO

Quantum-sized ZnO particles supported on sepiolite (ZnO/sepiolite) was prepared by sol-gel method using the sepiolite of acid activation as carrier, zinc acetate dihydrate (Zn(CH(3)COO)(2).2H(2)O) and lithium hydroxide monohydrate (LiOH.H(2)O) as raw material. The size of ZnO which supported on fibrous sepiolite is about 5 nm when calcined at 200 degrees C. The behavior of ZnO/sepiolite composites in the degradation of C.I. Reactive Blue 4 was investigated. Under the optimal preparation conditions, when the content of ZnO was about 70 wt.% in the nanocomposites, the photocatalytic property of the ZnO/sepiolite was excellent. The degradation rate of 20 mg/L C.I. Reactive Blue 4 could get to 98% in 120 min at room temperature when the concentration of catalyst was 0.2 g/L. The photocatalytic decomposition of organic pollutants accords with a pseudo first-order kinetic. The pH and H(2)O(2) influence the degradation rate of the ZnO/sepiolite. The experiment indicated that after the catalyst had been used 5 times repeatedly, the degradation rate had been still above 80%.


Assuntos
Silicatos de Magnésio/química , Teoria Quântica , Triazinas/química , Óxido de Zinco/química , Catálise , Estrutura Molecular , Tamanho da Partícula , Fotoquímica , Soluções , Propriedades de Superfície , Água/química
15.
Undersea Hyperb Med ; 36(3): 155-60, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19860137

RESUMO

Contradictory reports exist regarding the effects of hyperbaric oxygen (HBO2) on immune functions. We hypothesized that the intensity of exposure is a key factor, which leads to the different effects of HBO2 on immunity. In this study, we determined the function of rat splenic lymphocytes after in vitro exposure to different pressure-durations of HBO2. The proliferation rate and total cytotoxic T lymphocyte (CTL) activity stimulated by concanavalin A were enhanced by exposure to low (100 kPa for 60 or 90 minutes and 150 kPa for 30 or 60 minutes) HBO2 pressure-durations, but were inhibited by exposure to higher pressure-durations. We conclude that HBO2 exerts dual effects on lymphocyte function, as appropriate exposure promotes and excessive exposure inhibits the functions.


Assuntos
Proliferação de Células/efeitos dos fármacos , Oxigenação Hiperbárica , Oxigênio/farmacologia , Baço/citologia , Linfócitos T Citotóxicos/efeitos dos fármacos , Animais , Imunidade Celular/efeitos dos fármacos , Masculino , Ratos , Ratos Sprague-Dawley , Linfócitos T Citotóxicos/fisiologia , Fatores de Tempo
16.
Transplant Proc ; 41(5): 1749-55, 2009 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-19545721

RESUMO

OBJECTIVE: We evaluated the risk factors for biliary complications and surgical procedures for duct-to-duct reconstructions in adult living donor liver transplantation (LDLT). PATIENTS AND METHODS: From February 2005 to March 2008, we performed 100 cases of adult LDLT with duct-to-duct biliary reconstruction, using 64 right lobe grafts, 33 left lobe grafts, and 3 right lateral grafts. We employed 4 types of duct-to-duct procedures: all interrupted 6-0 Prolene suture (group 1, n = 9); continuous posterior and interrupted anterior wall 6-0 Prolene suture (group 2, n = 49); all continuous 7-0 Prolene suture (group 3, n = 26); and all continuous 7-0 Prolene suture with external stent (group 4, n = 16). Biliary complications were defined as an anastomosis stricture or a leakage. RESULTS: Thirty-four patients experienced biliary complications during the follow-up period (median, 27 months). The incidence of stricture was 27% and that of leakage, 8%. There were no perioperative, intraoperative, or anatomic risk factors for biliary complications, except the type of duct-to-duct procedure. Group 1 and 2 patients showed higher incidences of biliary strictures than groups 3 and 4 (43.1% vs 4.7%; P = .00). Group 3 patients experienced a higher incidence of bile leakage than the other groups (23.1% vs 2.7%; P = .004). CONCLUSIONS: The type of biliary reconstruction is a factor affecting biliary complications following duct-to-duct anastomosis in LDLT. Duct-to-duct biliary anastomosis with 7-0 monofilament suture and a small external stent is a feasible procedure in LDLT that significantly reduces the incidence of biliary complications.


Assuntos
Anastomose Cirúrgica/métodos , Ductos Biliares/cirurgia , Vesícula Biliar/cirurgia , Transplante de Fígado/efeitos adversos , Doadores Vivos , Complicações Pós-Operatórias/epidemiologia , Procedimentos Cirúrgicos Reconstrutivos/métodos , Adulto , Idoso , Ductos Biliares/patologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Seleção de Pacientes , Estudos Retrospectivos , Fatores de Risco
17.
Mater Res Bull ; 43(8-9): 2172-2178, 2008 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-19657381

RESUMO

Quantum-sized ZnO was prepared using sol-gel method with Zinc acetate dehydrate (Zn(CH(3)COO)(2).2H(2)O) and lithium hydroxide monohydrate(LiOH.H(2)O) as raw material. The ZnO particles annealed at different temperature were characterized by means of X-ray diffraction (XRD), Infrared absorption spectroscopy (IR) and UV-Vis spectroscopy. The degradation rate of reactive brilliant blue X-BR in aqueous solution was used to evaluate the photocatalytic performance of the quantum-sized ZnO. The experimental results indicated that the photocatalytic property of the ZnO was excellent. The photocatalytic efficiency of quantum-sized ZnO was significantly influenced by the calcining heat. When calcined at 300(o)C, its size is 6.78 nm and the photocatalytic performance is the best. The degradation rate of reactive brilliant blue X-BR could exceed 90% in 15 min at 35(o)C, when the concentration of the quantum-sized ZnO was 0.35 mg/L.

18.
J Gastroenterol Hepatol ; 10(2): 169-73, 1995.
Artigo em Inglês | MEDLINE | ID: mdl-7787163

RESUMO

A study was performed to evaluate radio-isotopic imaging using technetium-99m hexakis 2-methoxyisobutyl isonitrile administered per rectum to assess portal collateral circulation. The heart-liver ratios (H/L; mean +/- standard deviation) in 15 controls, 13 cases of histologically confirmed viral hepatitis and 57 cirrhosis patients were 0.27 +/- 0.11, 0.43 +/- 0.14 and 1.00 +/- 0.28, respectively (P < 0.001). Among the cirrhosis patients those with the Child-Pugh classification A, B and C had H/L of 0.56 +/- 0.14, 1.00 +/- 0.20 and 1.19 +/- 0.26, respectively (P < 0.001). A high value of H/L was associated with a high risk of hepatic encephalopathy (1.25 +/- 0.17, P < 0.01) and oesophageal varices (1.02 +/- 0.20, P < 0.01). There were associations between H/L and serum bilirubin (P < 0.01), albumin (P < 0.05) and prothrombin time (P < 0.05). The results also showed a good correlation between H/L and portal vein pressure measured during operation in 13 patients (P < 0.001, r = 0.87). The regression equation: y = 6.77 + 32.5 H/L, allowed portal vein pressure to be estimated. The prognostic value of the test was supported by the fact that good correlations were observed between the H/L ratio and widely accepted prognostic classification (Child-Pugh). It is suggested that this new method could be a reliable non-invasive way to give an indication of the degree of portasystemic shunting to evaluate the prognosis and to follow up the effects of medications for reducing portal hypertension in patients with cirrhotic portal hypertension.


Assuntos
Hipertensão Portal/diagnóstico por imagem , Cirrose Hepática/complicações , Tecnécio Tc 99m Sestamibi , Administração Retal , Adulto , Idoso , Circulação Colateral , Varizes Esofágicas e Gástricas/complicações , Feminino , Coração/diagnóstico por imagem , Encefalopatia Hepática/complicações , Hepatite Viral Humana/complicações , Humanos , Hipertensão Portal/complicações , Hipertensão Portal/fisiopatologia , Fígado/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Sistema Porta/fisiopatologia , Cintilografia , Tecnécio Tc 99m Sestamibi/administração & dosagem , Pressão Venosa
19.
Zhonghua Wai Ke Za Zhi ; 32(7): 395-7, 1994 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-7842972

RESUMO

A new method for evaluating portal systemic collaterals with administering 99mTc-MIBI per rectum was described in 80 cases. The heart to liver uptake ratio (H/L) in 15 healthy subjects was 0.267 +/- 0.1, slightly increased (0.455 +/- 0.13) in 8 patients with viral hepatitis and significantly increased in 68 cirrhotics. There was a good correlation between H/L ratio and the Child-Pugh classification with the ratio in Child A group of 0.57 +/- 0.12, in Child B group of 1.0 +/- 0.19, and in Child C group of 1.19 +/- 0.25 respectively (P < 0.01). For the diagnosis of portal hypertension, tentatively taking the ratio of > or = 0.62 as positive the sensitivity was 96%, specificity 89%, and accuracy 94%. The authors believe that 99mTc-MIBI imaging is a noninvasive method very useful in evaluating porta-systemic shunts.


Assuntos
Hipertensão Portal/diagnóstico por imagem , Tecnécio Tc 99m Sestamibi , Câmaras gama , Coração/diagnóstico por imagem , Humanos , Fígado/diagnóstico por imagem , Cirrose Hepática/diagnóstico por imagem , Cintilografia
20.
Anaesth Intensive Care ; 14(2): 158-62, 1986 May.
Artigo em Inglês | MEDLINE | ID: mdl-3740390

RESUMO

Hypertension after cardiac surgery is common and requires accurate control by carefully regulated infusions of drugs such as sodium nitroprusside. A microprocessor-based controller has been designed to close the loop between the blood pressure response and the infusion rate of a hypotensive drug. This system has been refined by computer simulation of the blood pressure response to sodium nitroprusside and by experience gained in using the controller in eleven patients in the early recovery period after cardiac surgery. The controller was able automatically to maintain blood pressure within 10% of a specified value. Provision of sophisticated safety features in automatic drug infusion controllers is essential for patient protection.


Assuntos
Computadores , Ferricianetos/administração & dosagem , Hipertensão/tratamento farmacológico , Microcomputadores , Monitorização Fisiológica/instrumentação , Nitroprussiato/administração & dosagem , Procedimentos Cirúrgicos Cardíacos , Humanos , Hipertensão/etiologia , Complicações Pós-Operatórias
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