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1.
Dev Comp Immunol ; 102: 103477, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31470020

RESUMO

Two continuous macrophage cell lines (LCM07 and LCM10) were established for the first time from the head kidney of the marine fish large yellow croaker (Larimichthys crocea). To date, both cell lines have been subcultured for more than 100 passages in 12 months. Notably, the LCM07 and LCM10 cells have distinct morphology and immune function. LCM07 cells showed strong contact inhibition in crowded conditions, while this was not observed in the LCM10 cells because they could grow in an overlapping manner. Correspondingly, LCM10 cells were slenderer than LCM07 cells. LCM07 cells had stronger phagocytic ability than LCM10 cells, while LCM10 cells had stronger respiratory burst activity after incubation with lipopolysaccharide (LPS) and phorbol ester (PMA). LCM07 cells had stronger Escherichia coli killing ability than LCM10 cells. The mRNA of macrophage markers, namely that of CD11b, CD114, CD68, CD86, CD209, and CD163, were all expressed in primary macrophages as well as the two cell lines. The mRNA expression levels of selected inflammatory cytokines, namely interleukin (IL)-1ß, IL-8, and tumor necrosis factor (TNF)α, were all upregulated after incubation with LPS. LPS also regulated key components of the mitogen-activated protein kinase (MAPK) signaling pathway, i.e., p38, ERK (extracellular signal-regulated kinase), and JNK (Jun N-terminal kinase) and their phosphorylated forms. Arachidonic acid (ARA) downregulated the LPS-induced upregulation of IL-1ß, IL-8, and TNFα, revealing that LCM07 and LCM10 cells are useful for studying nutritional immunity. In conclusion, two distinct macrophage cell lines have been established for the first time from the head kidney of marine fish, which could be useful for studying immunity and nutritional immunity.

2.
Mater Sci Eng C Mater Biol Appl ; 107: 110254, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31761216

RESUMO

Polymorphous light eruption (PLE) is one of the acquired idiopathic photodermatosis mainly induced by immoderate UV radiation. In order to realize UV protection and medicine administration simultaneously for polymorphous light eruption protection and therapy, Acetyl-11-keto-ß-boswellic acid (AKBA) loaded Zinc Oxide (ZnO) nanoparticles of which drug release behavior is UV-controlled has been successfully synthesized. Such nanoparticles can not only reflect UV but also transfer the energy to release AKBA which presents an excellent antioxidant and anti-inflammatory effects. In addition, they are biocompatible to HaCaT cells. As a result, they have a great potential in combining UV protection and medicine administration simultaneously for PLE protection and therapy.

3.
Talanta ; 206: 120183, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31514879

RESUMO

Mobility capillary electrophoresis (MCE) was developed previously in our group, which has the capabilities of ion separation and biomolecule hydrodynamic radius analysis. The coupling of MCE with mass spectrometry (MS) would greatly improve complex sample identification capability as well as system detection sensitivity. In the present study, a simple and robust ionization source, named as straight nano-electrospray ionization (nanoESI) source was developed, which was applied to couple MCE with MS. A stainless-steel needle attached directly at the end of an MCE capillary was used as the nanoESI emitter, and the connection between this emitter to the liquid flow in the MCE separation channel was established through a liquid bridge. After optimization, this straight nanoESI source enhanced the ion signal intensity by ~10 times when compared with a commercial nanoESI source. The MCE-straight nanoESI-MS system was also characterized in terms of mixture separation and peptide hydrodynamic radius measurements. Compared to our previous work when a UV detector was used in a commercial Lumex CE system (model Capel 105 M, St. Petersburg, Russia), peptides with much lower concentrations could be analyzed (from ~1 mg/mL to ~20 µg/mL) in terms of radius measurement.

4.
J Cell Biochem ; 121(1): 632-641, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31452251

RESUMO

Large intergenic noncoding RNA regulator of reprogramming (Linc-RoR) was first identified as a regulator to increase the emergence of induced pluripotent stem cells through reprogramming differentiated cells and is abnormal expression in a variety of malignant tumors. However, the function of Linc-RoR in pancreatic cancer progression needs further clarification. The data from this study demonstrated that Linc-RoR knockdown suppressed cell proliferative capacity and colony formation, while Linc-RoR overexpression promoted these behaviors. In particular, Linc-RoR overexpression promoted the level of mesenchymal markers, inhibited the expression of epithelial markers, as well as enhanced pancreatic cancer cells migration and invasion, whereas Linc-RoR knockdown inhibited the expression of mesenchymal markers, promoted the expression of epithelial markers, as well as weakened pancreatic cancer cells migration and invasion. Further study revealed that Linc-RoR knockdown brought about a significant fall in YAP phosphorylation and a rise in total YAP, while Linc-RoR overexpression produced the opposite results. Specifically, Linc-RoR promoted YAP in the cytoplasm into the nucleus. Taken together, we conjectured that Linc-RoR promoted proliferation, migration, and invasion of pancreatic cancer cells by activating the Hippo/YAP pathway. YAP might be an underlying target of Linc-RoR and mediate epithelial-mesenchymal transition (EMT) in pancreatic cancer (PC); thus, Linc-RoR might be a very meaningful biomarker for PC.

5.
J Ethnopharmacol ; 246: 112243, 2020 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-31541722

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Ginkgo biloba leaves and Panax ginseng are Chinese medicine commonly used in combination for cerebral disease. AIM OF THE STUDY: To investigate the effect of standard extract of Ginkgo biloba leaves (EGb) on facilitating brain uptake of ginsenoside and its underlying mechanisms. MATERIALS AND METHODS: The increasing uptake of ginsenosides in the brain of rats by EGb were detected by LC-MS/MS analysis. Evans blue and FITC-dextran leakage were determined to evaluate blood-brain barrier (BBB) permeability in vivo. Transendothelial electrical resistance (TEER) and Na-F penetration rate were measured with a co-culture of the human cerebral microvascular endothelial cell line (hCMEC/D3) and human normal glial cell line (HEB) in vitro BBB model. WB were used to analyzed the expression of BBB tight junctions (TJs) related protein (ZO-1, Occludin, Claudin-3, p-ERM, and p-MLC), ultrastructure of TJs was determined by transmission electron microscope. RESULTS: LC-MS/MS analysis demonstrated that EGb could improve brain uptake of ginsenoside Rg1, Re, Rd and Rb1. In vivo study showed that, BBB permeability was significantly increased after EGb administration, evidenced by the markedly increased penetration of FITC-dextran and Evans Blue into the mice brain parenchyma. In the in vitro BBB model, reduced TEER and increased Na-F penetration rate was observed in EGb group, which was associated with alteration of TJs ultrastructure. Furthermore, the expression of p-ERM and p-MLC in hCMEC/D3 as well as mice brain microvessels were significantly upregulated, but no significant change on the expression of TJs proteins (ZO-1, Occludin and Claudin-3). Moreover, the effect of EGb on in vitro BBB permeability and ERM, MLC phosphorylation was counteracted by DPCPX, an A1 adenosine receptor (A1R) antagonist. CONCLUSIONS: EGb might induce ERM/MLC phosphorylation and increase the cell-cell junction gaps to cause a reversible increase of the BBB permeability via A1R signaling pathway. Our results may contribute to better use of EGb in the treatment of brain diseases.

6.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 37(5): 563-567, 2019 Oct 01.
Artigo em Chinês | MEDLINE | ID: mdl-31721509

RESUMO

This case presents vertical root fracture with vital pulp in mandibular right first molar. Examinations of the history, clinical tests, laser Doppler flowmetry, and radiographs revealed that the tooth showed positive response to electric pulp testing and was normal compared with the healthy control tooth. This study aimed to use a novel vital preserving surgical technique (microapical surgery and nanometer bioactive materials) to make an effective therapeutic decision for the vital tooth with vertical root fracture.


Assuntos
Fraturas dos Dentes , Polpa Dentária , Humanos , Dente Molar , Raiz Dentária
7.
Int J Biol Macromol ; 2019 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-31726147

RESUMO

Gleditsia triacanthos polysaccharide, known as galactomannan, has not been exploited as a new functional material even though it possesses industrial potential in food and biomedicine. Galactomannans were recovered from the endosperm of seeds (15 weeks to 25 weeks after flowering) for deposition and maturation analysis. These galactomannans were characterized by using Nuclear magnetic resonance (NMR), X-ray diffraction (XRD), and monosaccharide composition analysis (particularly the mannose to galactose ratio) and molecular weight, solubility, and rheological measurements. The ratio of the three parts in mature seeds was as follows: endosperm (36.67%), hull (34.41%), and embryo (28.92%). The M/G ratio increased from 2.53 to 3.24 between 15 and 23 weeks and then decreased to 3.16 in 25 weeks, consistent with the trends of rheology and solubility. The molecular weight (1.28 × 106 g/mol) and intrinsic viscosity (882.53 mL/g) reached the maximum at 23 weeks and then decreased. Additionally, NMR and XRD showed that the M/G ratio did not change the basic chemical structure but caused slight changes in crystallinity. The purpose of the study was to reveal the changes in galactomannan structure, rheology, and solubility during G. triacanthos galactomannan deposition and maturation to facilitate exploration of its potential industrial applications.

8.
BMJ Open ; 9(11): e032462, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31727663

RESUMO

OBJECTIVE: This study aimed to assess household preparedness for emergency events and its determinants in China. DESIGN: A cross-sectional questionnaire survey was conducted on 3541 households in China in 2015. PARTICIPANTS: Households were selected using a stratified cluster sampling strategy, representing central, eastern, western and southern regions of China. The designed questionnaires were administered through face-to-face interviews. OUTCOME MEASURES: Household emergency preparedness was measured with 14 indicators, tapping into the supply of nine emergency necessities (food and water, extra batteries, battery-powered radio, battery-operated torch, first-aid kit, gas mask, fire extinguisher, escape ropes, whistle), coverage of accident insurance, knowledge of local emergency response systems (emergency numbers, exit routes and shelters) and availability of a household evacuation plan. If an individual acted on 9 of the 14 indicators, they were deemed well prepared. Logistic regression models were established to identify predictors of well preparedness based on 3541 returned questionnaires containing no missing values. RESULTS: Only 9.9% of households were well prepared for emergencies: 53.6% did not know what to do and 31.6% did not want to think about it. A higher level of preparedness was found in the respondents who have attained higher education (adjusted OR=0.826 compared with the higher level), participated in emergency training activities (adjusted OR=2.299), had better emergency knowledge (adjusted OR=2.043), reported less fate-submissiveness (adjusted OR=1.385) and more self-reliance (adjusted OR=1.349), prior exposure to emergency events (adjusted OR=1.280) and held more positive attitudes towards preparedness (adjusted OR=1.286). CONCLUSION: Household preparedness for emergency events is poor in China. Lack of motivation, negative attitude to preparedness and knowledge shortfall are major but remediable barriers for household preparedness.

9.
J Colloid Interface Sci ; 560: 186-197, 2019 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-31670016

RESUMO

Non-precious efficient bifunctional catalysts towards oxygen reduction/evolution reactions (ORR/OER) are highly desired to enable the widespread application of rechargeable Zn-air batteries (r-ZABs). Herein, Prussian blue analogues (PBA) anchored on CdS nanorods (CdS NRs) pre-coated with polydopamine (PDA) are utilized as precursors to prepare ultrafine Co4S3 nanoparticles supported on N, S-codoped CNTs (Co4S3@N,S-CNT), where CdS NRs are served as sulfur sources and hard templates. After pyrolysis, the resulting Co4S3@N,S-CNT-800 shows a high specific surface area of 142.4 m2 g-1, together with merely 0.780 V difference between the OER potential at 10 mA cm-2 and the ORR potential at 3 mA cm-2. The Co4S3@N,S-CNT-800 based air cathode displays a higher discharge capacity of 787 mAh gZn-1 at 10 mA cm-2, a higher output power density of 154 mW cm-2, better working stability, as well as a lower charge-discharge voltage gap than the Pt/C + RuO2 based air electrode at various working current density. The remarkable oxygen reversible catalytic activities are mainly attributed to the presence of a thin layer of mesoporous carbon on partial sections of the open-end N,S-CNTs, which not only shortens the mass diffusion length but also prevents N,S-CNTs from excessively bundling to maximize the exposure of Co4S3 nanocrystallites and graphitized carbon skeletons with N or S heteroatoms.

10.
Radiother Oncol ; 144: 13-22, 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31710939

RESUMO

PURPOSE: This study examines outcome heterogeneity and potential to refine the TNM-8 cN-classification using radiologic extranodal extension (rENE) in a contemporary HPV-positive (HPV+) oropharyngeal carcinoma (OPC) cohort. METHODS: All HPV+ OPC treated with definitive IMRT from 2010-2015 were included. Pre-treatment CT/MR of cN+ cases were reviewed by a head-neck radiologist for rENE. Overall survival (OS) and disease-free survival (DFS) were compared between rENE-positive (rENE+) vs rENE-negative (rENE-). Multivariable analysis (MVA) for OS confirmed the prognostic value of rENE. Refined cN-classifications for new TNM staging proposals were evaluated against TNM-8 using established criteria. RESULTS: A total of 517 cN+ (rENE+: 97; rENE-: 420) and 41 cN0 cases were identified. The rENE+ proportion increased with rising N-category (N1/N2/N3: 11%/19%/84%, p < 0.001). Median follow-up was 5.1 years. Compared to rENE-, rENE+ patients had a lower 5-year OS (56% vs 85%) and DFS (46% vs 83%) overall, and in N1 (OS: 57% vs 89%; DFS: 51% vs 87%) and N2 subsets (OS: 45% and 76%; DFS: 33% vs 74%) (all p < 0.001). MVA confirmed the prognostic value of rENE for OS (HR = 3.86, p < 0.001) and DFS (HR = 3.89, p < 0.001). We proposed two new cN-classifications: Schema1 reclassified any N_rENE+ as New_N3; Schema2 reclassified N1_rENE+ as New_N2 and N2_rENE+ as New_N3. Stage incorporating either Schema1 (ranked 1st) or Schema2 (ranked 2nd) cN-categories outperformed TNM-8. CONCLUSION: This study confirms that rENE is prognostically important and facilitates understanding of known outcome heterogeneity within TNM-8 in HPV+ OPC patients. rENE is a promising parameter to refine the TNM-8 cN-classifications.

11.
Int J Biol Macromol ; 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31730955

RESUMO

Similarities and differences of assembly for ovalbumin (OVA) and two kinds of sodium alginate (SA1 and SA2) varying in charge densities (λSA1: λSA2≈2:1) were investigated. The assembly processes of OVA/SA mixtures were characterized by phase diagram, particle size, and microstructure. Two differences between OVA/SA1 and OVA/SA2 mixtures in the phase diagram were distinctly observed. First, due to the higher charge density of SA1, the strong interaction between OVA and SA1 caused only pHφ1 to be recorded. A higher linear charge density of SA1 narrowed the pHφ1-pHφ2 range at ratios of 2:1 and 1:1. Second, OVA/SA1 complexes formed a coacervate with a relatively strong resistance to ion-induced shielding effects. This maintained the smaller size (tighter structure) with a larger number of complexes in the coacervate without 250 mM NaCl. The regulating polysaccharides with different charge densities could control the soluble region of complexes and endow various size or morphology of the coacervate assembled by proteins and polysaccharides.

12.
J Chem Phys ; 151(18): 184305, 2019 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-31731862

RESUMO

Using electron spectroscopy, we investigated the nanoplasma formation process generated in xenon clusters by intense soft x-ray free electron laser (FEL) pulses. We found clear FEL intensity dependence of electron spectra. Multistep ionization and subsequent ionization frustration features are evident for the low FEL-intensity region, and the thermal electron emission emerges at the high FEL intensity. The present FEL intensity dependence of the electron spectra is well addressed by the frustration parameter introduced by Arbeiter and Fennel [New J. Phys. 13, 053022 (2011)].

13.
Food Res Int ; 126: 108591, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31732077

RESUMO

Anthocyanins exist likely as complexes with flavones/metal ions in some plants. However, few studies indicate the complexation effects on bioactivities, i.e. antioxidant activities. The aim of this study was to evaluate effects of complexes of cyanidin-3-diglucoside-5-glucoside (CY3D5G) with rutin/Mg(II) on intrinsic antioxidant enzymes and mRNA expressions. All complexes exhibited cellular antioxidant activity in RAW264.7 cells, whereas half maximal effective concentration (EC50) values of CY3D5G complexes were significantly lower than its alone. Combination indexes of CY3D5G-rutin, CY3D5G-Mg(II), and CY3D5G-rutin-Mg(II) complexes were < 1 (p < .05), indicating of synergistic effects. Additionally, CY3D5G-Mg(II) rather than others significantly enhanced total antioxidant capacity, superoxide dismutase activity, and glutathione peroxidase activity while reducing malonic dialdehyde contents in H2O2-induced RAW264.7 cell model (p < 0.05), which were further confirmed with the up-regulated mRNA expression of nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1), and the down-regulated p21 (p < 0.05). Our results demonstrated that CY3D5G and its complexes might enhance cellular antioxidant activities via the Nrf2/HO-1 signaling pathway. Additionally, a synergistic antioxidant effect of CY3D5G in the present of rutin/ Mg(II) as regular food components was evident, suggesting anthocyanin might interact with dietary components such as Mg(II) or rutin in food matrix or biological systems and exhibit a stronger antioxidant activity than it alone. The Mg (II) that exhibited no antioxidant activities alone shows potential as a co-adjuvant for the anthocyanin antioxidant activity.

14.
BMC Musculoskelet Disord ; 20(1): 549, 2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31739778

RESUMO

BACKGROUND: Injuries in the musculoskeletal system, such as tendon and ligament ruptures, are challenging to manage and often require surgical reconstructions with limited long-term success. Thus, characterizations of these tissues are urgently needed to better understand cellular mechanisms that regulate tissue homeostasis and healing. Explant culturing systems allow for ex vivo analysis of tissues in an environment that mimics the native microenvironment in vivo. METHODS: Collaborative efforts within our institution facilitated the establishment of a novel explant culturing system. Tissue specimens cultured in single wells, with individual applied loading and/or biological environment, allowed characterization of tissue cultured under a variety of biological loading conditions. Quantitative PCR analysis for selected gene markers was our primary outcome. RESULTS: Data were stratified for analysis by either culture environment or loading condition. Our gene expression results show that specimens clustered by culture condition may differ in molecular markers related to ECM production (e.g., Col1a1, Adamts4) and/or organization (e.g., Tnc, Dnc). In contrast, loading condition did significantly alter the median gene expression levels of tissues in comparison to unloaded control samples, although gene expression values related to ECM degradation (e.g., Mmp1, Mmp10) were altered in tendons cultured under tension in the device. CONCLUSION: Our study demonstrates promising utility of a novel explant culturing system for further characterization of musculoskeletal tissues such as native tendons and ligaments, as well as pathologic fibrotic tissues resulting from arthrofibrosis or Dupuytren's disease.

15.
Sci Total Environ ; : 134876, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31740062

RESUMO

The bacterial communities on microplastics in marine and freshwater environments have been described by many studies. However, the migration and transportation processes of bacterial communities on microplastics in estuarine areas remain unclear. In this study, the bacterial communities on three substrates (microplastics, surface water and sediment) in estuarine areas (the Haihe Estuary (HHE) in Bohai Bay, China) were investigated based on 16S rRNA sequencing. The mean OTUs of the three substrates - water, microplastics and sediments - were 1091, 2213 and 3419, respectively. The partitioning of the OTUs among the three substrates indicated that the microplastics could be messengers facilitating the bacterial transportation between water and sediment. According to nMDS and relative abundance analyses, it was found that the microplastics enriched the particular bacteria (e.g., Halobacteriaceae and Pseudoalteromonadaceae) and weakened the influence of environmental variation. In addition, taxonomic and metabolic-pathway analyses indicated that the abundance of potentially pathogenic bacteria (e.g., Pseudomonas and Bacillus) on microplastics was significantly higher than that in the ambient environment. Meanwhile, the microplastic polymer types had little effect on the abundance and structure of the bacterial communities. Compared with surface water and sediments, microplastics could be a good habitat for bacterial communities and could lead to potential ecological risks because of the high stability, pathogenicity and stress tolerance of the bacterial communities on microplastics.

16.
Braz J Microbiol ; 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31667800

RESUMO

NADPH oxidases are enzymes that have been reported to generate reactive oxygen species (ROS) in animals, plants and many multicellular fungi in response to environmental stresses. Six genes of the NADPH oxidase complex components, including vvnoxa, vvnoxb, vvnoxr, vvbema, vvrac1 and vvcdc24, were identified based on the complete genomic sequence of the edible fungus Volvariella volvacea. The number of vvnoxa, vvrac1, vvbema and vvcdc24 transcripts fluctuated with ageing, and the gene expression patterns of vvnoxa, vvrac1 and vvbema were significantly positively correlated. However, the expression of vvnoxb and vvnoxr showed no significant difference during ageing. In hyphae subjected to mechanical injury stress, both O2- and H2O2 concentrations were increased. The expression of vvnoxa, vvrac1, vvbema and vvcdc24 was substantially upregulated, but vvnoxb and vvnoxr showed no response to mechanical injury stress at the transcriptional level. Additionally, the transcription of vvnoxa, vvrac1, vvbema and vvcdc24 could be repressed when the intracellular ROS were eliminated by diphenyleneiodonium (DPI) chloride and reduced glutathione (GSH) treatments. These results indicated a positive feedback loop involving NADPH oxidase and intracellular ROS, which might be the reason for the oxidative burst during injury stress.

17.
Chaos ; 29(10): 103150, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31675845

RESUMO

Recently, a large number of studies have concentrated on aging transition, but they have so far been restricted to coupled integer-order oscillators. Here, we report the first study of aging transition in mixed active and inactive fractional-order oscillators. It has been demonstrated that while the heterogeneity is caused by the distance parameter, both the coupling strength and the fractional-order derivative can modulate the critical ratio at which aging transition occurs. In addition, a small fractional-order derivative may ruin the ability of oscillation and, thus, reduce the critical ratio in globally coupled fractional-order Stuart-Landau oscillators. Remarkably, the larger the natural frequency is the more easily the aging transition occurs in coupled fractional-order oscillators. Further studies have shown that, being diverse from an integer-order Stuart-Landau oscillator, the natural frequency may induce a Hopf bifurcation in a fractional-order Stuart-Landau oscillator, accordingly, introducing a new heterogeneity in the coupled fractional-order Stuart-Landau oscillators. Therein, a counterintuitive phenomenon has been found that the critical ratio depends unmonotonously on the coupling strength, which implies that the coupled fractional-order Stuart-Landau oscillators possess the weakest robustness of oscillation at a certain level of coupling strength.

18.
Artigo em Inglês | MEDLINE | ID: mdl-31671669

RESUMO

Water resources utilization, social economy development, and ecological environment protection are key factors in regional sustainable development. Scientific evaluation of regional coordinated development status and diagnosis of regional uncoordinated development constraints will improve the management level of decision-makers. At present, most developing countries have the problem of unbalanced regional development caused by the one-sided pursuit of a certain system. Taking 14 prefecture-level cities in Hunan Province as cases, this paper analyzed the spatial and temporal distribution characteristics of the carrying capacity level of the water resources system, the development level of the social economy system and the protection level of the ecological environment system in each evaluation unit based on entropy weight method and order parameter analysis. Based on the theory of coordinated development, a calculation model of a coordinated development degree was constructed, and the corresponding evaluation criteria were formulated. The spatial and temporal distribution characteristics of a coordinated development degree in each research unit were analyzed and evaluated. The results showed that the average coordinated development degree of Hunan Province from 2004 to 2016 evolved from "Light disorder recession" to "Nearly disorder recession", then to "Reluctance coordinated development". Restricted by different systems, the coordinated development degree in each research unit presented spatial and temporal differences. According to different development stages and the characteristics of different regions, corresponding development strategies can be formulated to provide the guidance for coordinated the development of regions.

19.
Cancer Res Treat ; 2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31671936

RESUMO

Purpose: To investigate the prognostic impact of EBV-miR-BHRF1-1 with chronic lymphocytic leukemia (CLL) as well as role of EBV-miR-BHRF1-1 in p53 gene. Materials and Methods: Quantitative reverse transcription-PCR (qRT-PCR) and western blotting (WB) were used to quantify EBV-miR-BHRF1-1 and p53 expression in cultured CLL. Results: p53 aberration was associated with higher expression level of Epstein-Barr virus (EBV)-microRNA (miRNA, miR)-BHRF1-1 (p<0.001) which was also an independent prognostic marker for overall survival (OS) (p=0.028; HR 5.335 [1.193, 23.846]) in 97 newly-diagnosed CLL patients after adjusted with CLL-international prognostic index (CLL-IPI). We identified EBV-miR-BHRF1-1 as a viral miRNA regulator of p53. EBV-miR-BHRF1-1 repressed luciferase reporter activity by specific interaction with the seed region within the p53 3' untranslated region. Discordance of p53 messenger RNA and protein expression was associated with high EBV-miR-BHRF1-1 levels in CLL patients and cell lines. EBV-miR-BHRF1-1 inhibition upregulated p53 protein expression, induced cell cycle arrest and apoptosis and decreased cell proliferation in cell lines. EBV-miR-BHRF1-1 mimics downregulated p53 protein expression, decreased cell cycle arrest and apoptosis, and induced cell proliferation in cell lines. Conclusion: This study supported a role of EBV-miR-BHRF1-1 in p53 regulation in vitro. Our results support the potential of EBV-miR-BHRF1-1 as a therapeutic target in EBV-associated CLL with p53 gene aberration.

20.
Opt Lett ; 44(21): 5354-5357, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31675006

RESUMO

Flower-like silver vanadium oxide (SVO) micropatterns were realized by femtosecond laser in situ writing from its precursor. Self-assembled petals irradiated by a femtosecond laser were observed standing on the substrate along the scanned routine assisted by the formation of silver seeds and plasmonic-mediated effects. By controlling the concentration of ammonium monovanadate and the laser exposure time, a different thickness of petals was manipulated from ∼100 nm to micrometers. The SVO products were confirmed Ag4V2O7, AgVO3, and part of Ag3VO4 by x-ray diffraction (XRD) measurement. Photon-driven self-assembly for in situ fabrication of microstructures looks to be an effective and facile technique for SVO and other functional compounds.

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