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1.
Cell Death Dis ; 11(1): 76, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-32001670

RESUMO

Only a few types of inflammasomes have been described in central nervous system cells. Among these, the absent in melanoma 2 (AIM2) inflammasome is primarily found in neurons, is highly specific and can be activated only by double-stranded DNA. Although it has been demonstrated that the AIM2 inflammasome is activated by poly(deoxyadenylic-deoxythymidylic) acid sodium salt and leads to pyroptotic neuronal cell death, the role of AIM2 inflammasome-mediated pyroptosis in early brain injury (EBI) after subarachnoid haemorrhage (SAH) has rarely been studied. Thus, we designed this study to explore the mechanism of gasdermin D(GSDMD)-induced pyroptosis mediated by the AIM2 inflammasome in EBI after SAH. The level of AIM2 from the cerebrospinal fluid (CSF) of patients with SAH was detected. The pathway of AIM2 inflammasome-mediated pyroptosis, the AIM2/Caspase-1/GSDMD pathway, was explored after experimental SAH in vivo and in primary cortical neurons stimulated by oxyhaemoglobin (oxyHb) in vitro. Then, we evaluated GSDMD-induced pyroptosis mediated by the AIM2 inflammasome in AIM2 and caspase-1- deficient mice and primary cortical neurons generated through lentivirus (LV) knockdown. Compared with that of the control samples, the AIM2 level in the CSF of the patients with SAH was significantly increased. Pyroptosis-associated proteins mediated by the AIM2 inflammasome were significantly increased in vivo and in vitro following experimentally induced SAH. After AIM2 and caspase-1 were knocked down by an LV, GSDMD-induced pyroptosis mediated by the AIM2 inflammasome was alleviated in EBI after SAH. Intriguingly, when caspase-1 was knocked down, apoptosis was significantly suppressed via impeding the activation of caspase-3. GSDMD-induced pyroptosis mediated by the AIM2 inflammasome may be involved in EBI following SAH. The inhibition of AIM2 inflammasome activation caused by knocking down AIM2 and caspase-1 alleviates GSDMD-induced pyroptosis in EBI after SAH.

2.
Clin Rheumatol ; 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31950440

RESUMO

The first name of the co-author of the above article was presented incorrect in the published version. The author name "Miangliang Qiu" should read "Mingliang Qiu" as mentioned above.

3.
J Surg Res ; 245: 321-329, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31421380

RESUMO

In the adult rodents' brain, CD24 expression is restricted to immature neurons located in the neurogenesis areas. Our previous studies have confirmed that CD24 expression could be markedly elevated in the cerebral cortex after traumatic brain injury (TBI) both in humans and in mice. Although there is a close relationship between CD24 and neurogenesis, it remains unknown about the specific role of CD24 in neurogenesis areas after TBI. Here, the expression of CD24 was detected in the ipsilateral hippocampus by the Western blotting and real-time quantitative polymerase chain reaction. RNA interference was applied to investigate the effects of CD24 on post-traumatic neurogenesis. Brain sections were labeled with CD24 and doublecortin (DCX) via immunofluorescence. The Morris water maze test was used to assess cognitive functions. The results indicated that both mRNA and protein levels of CD24 were markedly elevated in the hippocampus after TBI. Meanwhile, TBI could cause a decrease of DCX-positive cells in the dentate gyrus of the hippocampus. Downregulation of CD24 significantly inhibited the phosphorylation of Src homology region 2-containing protein tyrosine phosphatase 2 in the ipsilateral hippocampus. Meanwhile, inhibition of CD24 could reduce the number of DCX-positive cells in the dentate gyrus area and impair cognitive functions of the TBI mice. These data suggested that hippocampal expression of CD24 might positively regulate neurogenesis and improve cognitive functions after TBI.


Assuntos
Lesões Encefálicas Traumáticas/fisiopatologia , Antígeno CD24/metabolismo , Cognição/fisiologia , Hipocampo/fisiopatologia , Neurogênese/fisiologia , Animais , Antígeno CD24/genética , Modelos Animais de Doenças , Regulação para Baixo , Humanos , Masculino , Aprendizagem em Labirinto , Camundongos , Neurônios/fisiologia , RNA Interferente Pequeno/metabolismo , Recuperação de Função Fisiológica , Regulação para Cima
4.
Clin Rheumatol ; 2019 Dec 26.
Artigo em Inglês | MEDLINE | ID: mdl-31879859

RESUMO

OBJECTIVE: miR-150-5p has been implicated in the regulation and onset of immune diseases. We investigated the effects of miR-150-5p on the functions of RA synovial fibroblasts (RASFs). METHOD: The binding site between suppressor of cytokine signaling 1 (SOCS1) and miR-150-5p was analyzed using European Bioinformatics Institute database, and the 3' UTR of SOCS1 mRNA, including the binding site, was amplified and ligated to the 3'-end of LUC2 gene in the pmirGL0 dual-luciferase vector. The pmirGL0 vector and corresponding mimics were subsequently co-transfected into 293T cells to compare the relative fluorescence intensity of LUC2 between the miR-150-5p mimics and the negative control (NC) mimics groups. Further, the RASF cell line MH7A was transfected with miR-150-5p or NC mimics and subjected to flow cytometric analysis, cell counting kit-8 assay, western blot analysis, qPCR, and enzyme-linked immunosorbent (ELISA) assay 48 h after transfection. RESULTS: miR-150-5p mimics resulted in a lower cell apoptotic rate and proportion of cells in the S phase. Using a dual-luciferase reporter gene assay, we then found that SOCS1 is a potential target of miR-150-5p. Compared with NC mimics, miR-150-5p mimics significantly decreased the protein and mRNA expression levels of SOCS1. ELISA assay showed that miR-150-5p mimics increased interleukin-6 level in the cell culture medium but did not influence tumor necrosis factor-alpha levels. CONCLUSIONS: Overall, the growth-promoting effect of miR-150-5p on MH7A cells may be attributed to the miR-150-5p-induced degradation of SOCS1 mRNA, suggesting a potential therapeutic target for RA.Key Points• SOCS1 is a potential target of miR-150-5p.• miR-150-5p promoted the growth of RASF cell line MH7A.• miR-150-5p increased the secretion of IL-6 but did not significantly affect TNF-α levels in MH7A cells.

5.
Artigo em Inglês | MEDLINE | ID: mdl-31740120

RESUMO

Yeast is one of the most widely used microbial species in the field of microbiology, and it is crucial that rapid and accurate monitoring of its process. Therefore, this study presents a method using Raman spectroscopy for quantitative analysis of yeast fermentation process. First, a ProSP-Micro2000K Raman measuring system used to obtain the Raman spectra of eight batches of yeast samples during fermentation, and the spectra obtained were pretreated using Savitzky-Golay (SG) smoothing filter and standard normal variate (SNV). Then, two variable selection methods, which were competitive adaptive reweighted sampling (CARS) and variable combination population analysis (VCPA), were compared to search the preprocessed Raman spectroscopy characteristic wavenumber. Finally, support vector machine (SVM) was employed to construct a quantitative monitoring model of yeast fermentation process based on variables from the selected characteristic wavenumbers. The results revealed that the VCPA-SVM model showed the best prediction result with 14 selected characteristic wavelength variables. The coefficient of determination (RP2) of the optimal model was 0.979, while the root mean square error of prediction (RMSEP) was 0.108 in the validation set. The overall results demonstrate that the Raman spectroscopy integrated with chemometric approaches could be utilized as a rapid method to monitor the process of yeast cultivations.

6.
Food Res Int ; 126: 108605, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31732085

RESUMO

Aroma is an important index to evaluate the quality and grade of black tea. This work innovatively proposed the sensory evaluation of black tea aroma quality based on an olfactory visual sensor system. Firstly, the olfactory visualization system, which can visually represent the aroma quality of black tea, was assembled using a lab-made color sensitive sensor array including eleven porphyrins and one pH indicator for data acquisition and color components extraction. Then, the color components from different color sensitive spots were optimized using the particle swarm optimization (PSO) algorithm. Finally, the back propagation neural network (BPNN) model was developed using the optimized characteristic color components for the sensory evaluation of black tea aroma quality. Results demonstrated that the BPNN models, which were developed using three color components from FTPPFeCl (component G), MTPPTE (component B) and BTB (component B), can get better results based on comprehensive consideration of the generalization performance of the model and the fabrication cost of the sensor. In the validation set, the average of correlation coefficient (RP) value was 0.8843 and the variance was 0.0362. The average of root mean square error of prediction (RMSEP) was 0.3811 and the variance was 0.0525. The overall results sufficiently reveal that the optimized sensor array has promising applications for the sensory evaluation of black tea products in the process of practical production.

7.
Polymers (Basel) ; 11(10)2019 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-31614825

RESUMO

The morphology of the active layer plays a crucial role in determining device performance and stability for organic solar cells. All-polymer solar cells (All-PSCs), showing robust and stable morphologies, have been proven to give better thermal stability than their fullerene counterparts. However, outstanding thermal stability is not always the case for polymer blends, and the limiting factors responsible for the poor thermal stability in some All-PSCs, and how to obtain higher efficiency without losing stability, still remain unclear. By studying the morphology of poly [2,3-bis (3-octyloxyphenyl) quinoxaline-5,8-diyl-alt-thiophene-2,5-diyl](TQ1)/poly[4,8-bis[5-(2-ethylhexyl)-2-thienyl]benzo[1,2-b:4,5-b']dithiophene-alt-(4-(2-ethylhexyl)-3-fluorothieno[3,4-b]thiophene-)-2-carboxylate-2-6-diyl]] (PCE10)/PNDI-T10 blend systems, we found that the rearranged molecular packing structure and phase separation were mainly responsible for the poor thermal stability in devices containing PCE10. The TQ1/PNDI-T10 devices exhibited an improved PCE with a decreased π-π stacking distance after thermal annealing; PCE10/PNDI-T10 devices showed a better pristine PCE, however, thermal annealing induced the increased π-π stacking distance and thus inferior hole conductivity, leading to a decreased PCE. Thus, a maximum PCE could be achieved in a TQ1/PCE10/PNDI-T10 (1/1/1) ternary system after thermal annealing resulting from their favorable molecular interaction and the trade-off of molecular packing structure variations between TQ1 and PCE10. This indicates that a route to efficient and thermal stable All-PSCs can be achieved in a ternary blend by using material with excellent pristine efficiency, combined with another material showing improved efficiency under thermal annealing.

8.
BMC Cancer ; 19(1): 949, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31615459

RESUMO

BACKGROUND: In the quest for new anti-cancer drugs, the drug discovery process has shifted to screening of active ingredients in traditional eastern medicine. Matrine is an active alkaloid isolated from plants of the Sophora genus used in traditional Chinese herbal medicine that exhibits a wide spectrum of biological properties and has a potential as an anti-proliferative agent. In this study, we investigated the anticancer property of MASM, ([(6aS, 10S, 11aR, 11bR, 11cS)210-Methylamino-dodecahydro-3a, 7a-diaza-benzo (de)anthracene-8-thione]), a potent derivative of matrine. METHODS: Four epithelial cancer cell lines representing the dominant cancers, namely: A549 (non-small-cell lung cancer cell line), MCF-7 and MDA-MB-231 (breast cancer cell lines), and Hela (cervical cancer cell line) were employed, and the mechanistic underpinning of MASM-induced apoptosis was investigated using flow cytometry, western blot and immunofluorescence. RESULTS: MASM, induced apoptosis via caspase 3 dependent and independent pathways, and autophagy in all the four cancer cell lines, but post-EMT (epithelial mesenchymal transition) cells showed greater sensitivity to MASM. Scavenging reactive oxygen species using N-acetylcysteine rescued all cancer cell lines from apoptosis and autophagy. Mechanistic analysis revealed that MASM induced autophagy involves inhibition of Akt signaling and the activation of Erk and p38 signaling, and inhibition of autophagy further enhanced the apoptosis induced by MASM. CONCLUSIONS: These results indicate that MASM possesses potency against cancer cells and modulating autophagy during MASM administration could be used to further enhance its therapeutic effects.

9.
Front Neurosci ; 13: 989, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31619957

RESUMO

Astaxanthin (ATX) is a carotenoid that exerts strong anti-oxidant and anti-inflammatory property deriving from its highly unsaturated molecular structures. However, the low stability and solubility of ATX results in poor bioavailability, which markedly hampers its application as therapeutic agent in clinic advancement. This study investigated a promising way of transferrin conjugated to poly (ethylene glycol) (PEG)-encapsulated ATX nanoparticles (ATX-NPs) on targeted delivery and evaluated the possible mechanism underlying neuroprotection capability. As a result, the ATX integrated into nanocarrier presented both well water-dispersible and biocompatible, primely conquering its limitations. More than that, the transferrin-containing ATX-NPs exhibited enhanced cellular uptake efficiency than that of ATX-NPs without transferrin conjugated in primary cortical neurons. Additionally, compared to free ATX, transferrin-containing ATX-NPs with lower ATX concentration showed powerful neuroprotective effects on OxyHb-induced neuronal damage. Taken together, the improved bioavailability and enhanced neuroprotective effects enabled ATX-NPs as favorable candidates for targeted delivery and absorption of ATX. We believe that these in vitro findings will provide insights for advancement of subarachnoid hemorrhage therapy.

10.
Cancer Med ; 8(16): 6904-6914, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31568654

RESUMO

BACKGROUND: To examine the microbial profiles in parenchyma tissues in bladder cancer. METHODS: Tissue samples of cancerous bladder mucosa were collected from patients diagnosed with bladder cancer (22 carcinoma tissues and 12 adjacent normal tissues). The V3-V4 region of the bacterial 16S rRNA gene was PCR amplified, followed by sequencing on an Illumina MiSeq platform. Bioinformatics analysis for microbial classification and functional assessment was performed to assess bladder microbiome diversity and variations. RESULTS: The predominant phylum in both tissues was Proteobacteria. The cancerous tissues exhibited lower species richness and diversity. Beta diversity significantly differed between the cancerous and normal tissues. Lower relative abundances of the microbial genera Lactobacillus, Prevotella_9, as well as Ruminococcaceae were observed, whereas those of Cupriavidus spp., an unknown genus of family Brucellaceae, and Acinetobacter, Anoxybacillus, Escherichia-Shigella, Geobacillus, Pelomonas, Ralstonia, and Sphingomonas were higher in the cancerous tissues. These findings indicate that these genera may be potentially utilized as biomarkers for bladder cancer. PICRUSt analysis revealed that several pathways involved in the metabolism of harmful chemical compounds were enriched in the cancer tissues, thereby providing evidence that environmental factors are strongly associated with bladder cancer etiology. CONCLUSION: This is the first study that has described and analyzed the dysbiotic motifs of urinary microbiota in the parenchymatous tissues of bladder cancer via 16S rRNA gene sequencing. Our results suggest that changes in the bladder microbiome may serve as biomarkers for bladder cancer, possibly assisting in disease screening and monitoring.

11.
J Cell Mol Med ; 23(11): 7664-7672, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31490024

RESUMO

MicroRNA (miRNA) critically controls gene expression in many biological processes, including lung growth and pulmonary surfactant biosynthesis. The present study was conducted to investigate whether miR-20a-5p had such regulatory functions on alveolar type II (AT-II) cells. To accomplish this, miR-20a-5p-overexpressed and miR-20a-5p-inhibited adenoviral vectors were constructed and transfected into cultured AT-II cells that were isolated from rat foetal lungs of 19 days' gestation. Transfection efficiency was confirmed by observing the fluorescence of green fluorescent protein (GFP) carried by the viral vector, whereas miR-20a-5p levels were verified by real-time PCR. The CCK-8 assay was used to compare the proliferation ability of AT-II cells that had over- or underexpressed miR-20a-5p. The expression of surfactant-associated proteins (SPs) and phosphatase and tensin homolog (PTEN) was measured by real-time PCR and Western blotting. In AT-II cells, transfection resulted in over- or under-regulation of miR-20a-5p. While overexpression of miR-20a-5p promoted pulmonary surfactant gene expression, its underexpression inhibited it. Consistent with its role in negatively regulating the pulmonary surfactant gene, an opposite pattern was observed for miR-20a-5p regulation of PTEN. As a result, when miR-20a-5p was rendered overexpressed, PTEN was down-regulated. By contrast, when miR-20a-5p was underexpressed, PTEN was up-regulated. Neither overexpression nor underexpression of miR-20a-5p altered the cell proliferation. miR-20a-5p plays no role in proliferation of foetal AT-II cells but is a critical regulator of surfactant gene expression. The latter appears to be achieved through a regulatory process that implicates expression of PTEN.

12.
Iran J Immunol ; 16(2): 170-181, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31182691

RESUMO

BACKGROUND: Realgar, an arsenic tetrasulfide compound, is a highly recognized traditional Chinese medicinal prescription that has been widely used to treat various diseases such as inflammatory diseases. However, there are still some problems in the clinical treatment of Realgar, such as large oral dose and high potential toxicity. OBJECTIVE: To evaluate effects of Realgar nanoparticles on lupus nephritis (LN) in vivo in MRL/lpr mice. METHODS: Ten-week mice were orally administered every day for eight consecutive weeks except the mice of normal model groups. The serum levels of anti-ds-DNA antibody IgG, IgM, IFN-γ, Creatinine (Cr), and blood urea nitrogen (BUN) were determined, and 24-hour urine protein was also measured. Renal inflammatory pathology analysis was assessed by hematoxylin-eosin (H&E) staining. The expression of phosphorylated signal transducer and activator of transcription 1 (p-STAT 1) and Janus Kinase 1 (JAK 1) in kidney tissue was determined by direct reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC). RESULTS: The mice treated with Realgar nanoparticle in the high dose-treated (Realgar HD, 0.03 g/kg/d) group exhibited significantly reduced serum levels of anti-dsDNA (p<0.01), IgG (p<0.01), IgM (p<0.01), BUN (p<0.01), Cr (p<0.01), and inflammatory cytokine IFN-γ (p<0.01) as well as proteinuria (p<0.01) compared to the untreated model MRL/lpr mice. Additionally, high doses of Realgar nanoparticles significantly suppressed the phosphorylations of STAT 1(p<0.01) and the renal pathological changes. CONCLUSIONS: The study indicates that Realgar nanoparticles may be a potential agent to treat LN, and the down-regulated p-STAT1 expression suggests that it may be one of the LN treatment targets for Realgar nanoparticles.


Assuntos
Arsenicais/uso terapêutico , Rim/patologia , Nefrite Lúpica/terapia , Nanopartículas/uso terapêutico , Fator de Transcrição STAT1/metabolismo , Sulfetos/uso terapêutico , Animais , Arsenicais/química , Citocinas/metabolismo , Modelos Animais de Doenças , Feminino , Humanos , Mediadores da Inflamação/metabolismo , Janus Quinase 1/metabolismo , Medicina Tradicional Chinesa , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos MRL lpr , Nanopartículas/química , Transdução de Sinais , Sulfetos/química
13.
World Neurosurg ; 130: 1-6, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31254713

RESUMO

BACKGROUND: Cases of multiple intracranial tumors are common; however, cases of multiple intraspinal tumors are rare. Except for cases of neurofibromatosis, it is very rare for tumors of different pathological types to exist concurrently at the same spinal level. Only 9 cases have been reported to date, with meningioma found with schwannoma in 7 cases and with neurofibroma in 2 cases. CASE DESCRIPTION: We have reported another rare case in which neurofibroma and meningioma were identified within a single dumbbell-shaped tumor at the same cervical level without neurofibromatosis. The preoperative magnetic resonance imaging findings indicated a single extra- and intradural extramedullary dumbbell-shaped neurogenic tumor on the left ventral side of the cervical spine. Intraoperatively, we found that the mass consisted of 2 pathologically different tumors. The results of surgical resection were mostly satisfactory. CONCLUSIONS: To the best of our knowledge, the present case is the first reported case of intradural neurofibroma (not meningioma) and extradural meningioma growing mixed together at the same spinal level without neurofibromatosis. The precise mechanism underlying the formation of the tumor is unknown, and multidirectional differentiation of a common progenitor cell is one possibility. Intra- and extradural exploration and component biopsies are useful for treatment planning, especially when the magnetic resonance imaging is not sufficiently sensitive for the diagnosis of coexisting tumor types.


Assuntos
Vértebras Cervicais/diagnóstico por imagem , Neoplasias Meníngeas/diagnóstico por imagem , Meningioma/diagnóstico por imagem , Neurofibroma/diagnóstico por imagem , Neurofibromatoses , Neoplasias da Coluna Vertebral/diagnóstico por imagem , Vértebras Cervicais/cirurgia , Feminino , Humanos , Neoplasias Meníngeas/complicações , Neoplasias Meníngeas/cirurgia , Meningioma/complicações , Meningioma/cirurgia , Pessoa de Meia-Idade , Neurofibroma/complicações , Neurofibroma/cirurgia , Neoplasias da Coluna Vertebral/complicações , Neoplasias da Coluna Vertebral/cirurgia
14.
Nat Commun ; 10(1): 2818, 2019 Jun 27.
Artigo em Inglês | MEDLINE | ID: mdl-31249295

RESUMO

Metal halide perovskites are emerging as promising semiconductors for cost-effective and high-performance light-emitting diodes (LEDs). Previous investigations have focused on the optimisation of the emissive perovskite layer, for example, through quantum confinement to enhance the radiative recombination or through defect passivation to decrease non-radiative recombination. However, an in-depth understanding of how the buried charge transport layers affect the perovskite crystallisation, though of critical importance, is currently missing for perovskite LEDs. Here, we reveal synergistic effect of precursor stoichiometry and interfacial reactions for perovskite LEDs, and establish useful guidelines for rational device optimization. We reveal that efficient deprotonation of the undesirable organic cations by a metal oxide interlayer with a high isoelectric point is critical to promote the transition of intermediate phases to highly emissive perovskite films. Combining our findings with effective defect passivation of the active layer, we achieve high-efficiency perovskite LEDs with a maximum external quantum efficiency of 19.6%.

15.
PeerJ ; 7: e7103, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31205825

RESUMO

Background: Autophagy is an important mechanism to maintain homeostasis in cells. It has been linked with ageing and many currently incurable diseases, including heart disease, cancer, myopathies, neurodegeneration, and diabetes. Autophagy research is very important for identifying better treatments. This study aimed to explore the hotspots of autophagy research published from different countries, organizations, and authors. Methods: Between 1962 and 2018, articles published about autophagy were identified in the Web of Science database. The total and annual number of articles, citations, impact factor, Hirsch (H)-index, number of article citations, productive authors, and involved journals were collected for quantitative and qualitative comparisons. Results: From 1962 to 2018, 18,811 autophagy-related articles written in English were published. Most were from China (6,731). The United States dominated in citation frequency (391,030) and h-index (264). Among related journals, Autophagy published the most articles (1,388), followed by Plos One (585) and Oncotarget (392). Daniel Klionsky was the most productive author, with 171 publications. The article "LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing" was cited most frequently. The top-ranked keyword was "degradation" of macroautophagy. Conclusions: Publication of articles about autophagy has increased notably from 1962 to 2018, and has increased annually. The general quality of publications from China is still in need of improvement. Autophagy research has shifted gradually from basic studies to clinical studies in recent years.

16.
Sensors (Basel) ; 19(9)2019 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-31052151

RESUMO

The odor information produced in Saccharomyces cerevisiae culture is one of the important characteristics of yeast growth status. This work innovatively presents the quantitative monitoring of cell concentration during the yeast culture process using a homemade color sensor. First, a color sensor array, which could visually represent the odor changes produced during the yeast culture process, was developed using eleven porphyrins and one pH indicator. Second, odor information of the culture substrate was obtained during the process using the homemade color sensor. Next, color components, which came from different color sensitive spots, were extracted first and then optimized using the ant colony optimization (ACO) algorithm. Finally, the back propagation neural network (BPNN) model was developed using the optimized feature color components for quantitative monitoring of cell concentration. Results demonstrated that BPNN models, which were developed using two color components from FTPPFeCl (component B) and MTPPTE (component B), can obtain better results on the basis of both the comprehensive consideration of the model performance and the economic benefit. In the validation set, the average of determination coefficient R P 2 was 0.8837 and the variance was 0.0725, while the average of root mean square error of prediction (RMSEP) was 1.0033 and the variance was 0.1452. The overall results sufficiently demonstrate that the optimized sensor array can satisfy the monitoring accuracy and stability of the cell concentration in the process of yeast culture.


Assuntos
Técnicas Biossensoriais , Técnicas de Cultura de Células , Rastreamento de Células , Saccharomyces cerevisiae/isolamento & purificação , Algoritmos , Cor , Concentração de Íons de Hidrogênio , Porfirinas/química , Saccharomyces cerevisiae/crescimento & desenvolvimento
17.
PeerJ ; 7: e6825, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31086745

RESUMO

Objectives: Rheumatology-related diseases remain a significant burden worldwide. However, little is known about the comparative status of rheumatology research between Mainland China (MC) and the world's leading countries. The aim of this study is to compare the quantity and quality of research output in the field of rheumatology that were written by researchers from MC, the USA, the UK, the Netherlands and France. Methods: Between 2007 and 2017, all articles published in 30 rheumatology journals were identified via Science Citation Index Expanded database. The number of total and annual articles, article types (randomized controlled trials (RCTs), reviews, case reports, clinical trials and meta-analysis), impact factor (IF), citations, h-index and articles in the high-impact journals were collected for quantity and quality comparisons. The correlation of socioeconomic factors and annual publications was also analyzed. Results: From 2007 to 2017, there were 53,439 articles published in rheumatology journals, of which researchers from the USA published 13,391 articles, followed by the UK, the Netherlands, France and MC with 6,179, 4,310, 4,066 and 2,898 articles, respectively. Publications from MC represented the ninth, but the number is growing rapidly. For total and average citations, MC still lags behind the other four countries in the study. Similar trends were observed in average IF, h-index and articles in the high-impact journals. In terms of article types, the USA occupies the dominant place, except for meta-analysis. The annual numbers of articles from MC and the USA were positively correlated with gross domestic product (p < 0.05). Conclusions: The USA has played predominant role in rheumatology research for the last 11 years. The annual number of published articles from MC has increased notably from 2007 to 2017. Although MC has made progress in the number of published articles over the past decade, it still lags far behind the highly developed countries in most bibliometric indicators. Thus, the general quality of publications from MC needs further improvement.

18.
Hum Gene Ther ; 30(9): 1117-1132, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31126191

RESUMO

In an effort to develop a new therapy for cancer and to improve antiprogrammed death inhibitor-1 (anti-PD-1) and anticytotoxic T lymphocyte-associated protein (anti-CTLA-4) responses, we have created a telomerase reverse transcriptase promoter-regulated oncolytic adenovirus rAd.sT containing a soluble transforming growth factor receptor II fused with human IgG Fc fragment (sTGFßRIIFc) gene. Infection of breast and renal tumor cells with rAd.sT produced sTGFßRIIFc protein with dose-dependent cytotoxicity. In immunocompetent mouse 4T1 breast tumor model, intratumoral delivery of rAd.sT inhibited both tumor growth and lung metastases. rAd.sT downregulated the expression of several transforming growth factor ß (TGFß) target genes involved in tumor growth and metastases, inhibited Th2 cytokine expression, and induced Th1 cytokines and chemokines, and granzyme B and perforin expression. rAd.sT treatment also increased the percentage of CD8+ T lymphocytes, promoted the generation of CD4+ T memory cells, reduced regulatory T lymphocytes (Tregs), and reduced bone marrow-derived suppressor cells. Importantly, rAd.sT treatment increased the percentage of CD4+ T lymphocytes, and promoted differentiation and maturation of antigen-presenting dendritic cells in the spleen. In the immunocompetent mouse Renca renal tumor model, similar therapeutic effects and immune activation results were observed. In the 4T1 mammary tumor model, rAd.sT improved the inhibition of tumor growth and lung and liver metastases by anti-PD-1 and anti-CTLA-4 antibodies. Analysis of the human breast and kidney tumors showed that a significant number of tumor tissues expressed high levels of TGFß and TGFß-inducible genes. Therefore, rAd.sT could be a potential enhancer of anti-PD-1 and anti-CTLA-4 therapy for treating breast and kidney cancers.

19.
Food Funct ; 10(4): 1940-1947, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30874277

RESUMO

Vitexin, a bioactive compound isolated from hawthorn leaf extracts, has been reported to exhibit many biological activities, such as anticancer, antioxidation, and adipogenesis inhibition activities. The current study explored the effects of vitexin on high fat diet (HFD)-induced obesity/adipogenesis in male C57BL/6J mice and 3T3-L1 adipocytes, as well as the underlying mechanisms thereof. Vitexin significantly mitigated HFD-induced body weight gain and adiposity. Vitexin also partially normalized serum, hepatic lipid contents, and decreased adipocyte size induced by the HFD. Consistently, there were significant effects of vitexin on important regulators of lipid metabolism, including AMP-activated protein kinase-α (AMPKα), CAATT element binding protein-α (C/EBPα), and fatty acid synthase (FAS) in white adipose tissue. Moreover, vitexin significantly inhibited fat accumulation in 3T3-L1 adipocytes, and this was totally abolished by compound C (an AMPKα inhibitor). These results suggest that vitexin may prevent HFD-induced obesity/adipogenesis via the AMPKα mediated pathway.


Assuntos
Proteínas Quinases Ativadas por AMP/metabolismo , Fármacos Antiobesidade/administração & dosagem , Apigenina/administração & dosagem , Obesidade/tratamento farmacológico , Células 3T3-L1 , Proteínas Quinases Ativadas por AMP/genética , Adipócitos/efeitos dos fármacos , Adipócitos/metabolismo , Animais , Proteína alfa Estimuladora de Ligação a CCAAT/genética , Proteína alfa Estimuladora de Ligação a CCAAT/metabolismo , Dieta Hiperlipídica/efeitos adversos , Ácido Graxo Sintases/genética , Ácido Graxo Sintases/metabolismo , Humanos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Obesidade/etiologia , Obesidade/genética , Obesidade/metabolismo , Transdução de Sinais/efeitos dos fármacos
20.
Mol Med Rep ; 19(4): 3237-3246, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30816502

RESUMO

Ankylosing spondylitis (AS) is an autoimmune condition characterized by chronic inflammation and abnormal ossification as the primary features of the disease. The aim of the present study was to investigate the role of C­X­C chemokine receptor type 4 (CXCR4) in ossification from patients with AS. CXCR4 expression was assessed by western blot analysis and immunohistochemistry analysis of tissues obtained from patients with AS and controls. Fibroblasts were isolated, cultured and incubated with AMD 3100 and stromal cell­derived factor­1 to inhibit and promote CXCR4 levels, respectively. CXCR4 was upregulated in hip synovial tissues from patients with AS compared with that observed in controls. AS fibroblasts exhibited increased proliferation and growth rates. Inhibition of CXCR4 increased the phosphorylation of ß­catenin and downregulated the expression of ß­catenin, v­myc avian myelocytomatosis viral oncogene homolog, cyclin D1 and osteocalcin. Alizarin red staining demonstrated a decrease in biomineralization activity following the inhibition of CXCR4. These data support the hypothesis that inhibiting CXCR4 in patients with AS may suppress the ossification of fibroblasts.


Assuntos
Diferenciação Celular/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Osteogênese/efeitos dos fármacos , Receptores CXCR4/antagonistas & inibidores , Via de Sinalização Wnt/efeitos dos fármacos , Adulto , Idoso , Biomarcadores , Diferenciação Celular/genética , Proliferação de Células , Feminino , Fibroblastos/citologia , Expressão Gênica , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Osteogênese/genética , Receptores CXCR4/genética , Receptores CXCR4/metabolismo
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