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1.
Dev Comp Immunol ; 106: 103611, 2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-31953153

RESUMO

In grass carp (Ctenopharyngodon idella), septicemia is a systemic inflammatory response to bacterial infection. Once infected bacteria, a hyper-inflammatory state that could lead to septic shock and death. There is increasing evidence that microRNAs are involved in the regulation of the inflammatory response. Ctenopharyngodon idella growth arrest and DNA damage-inducible 45 ba and bb (CiGadd45ba and CiGadd45bb) are two subtypes of Gadd45b. In the present study, miR-148 was confirmed to be involved in the inflammatory response after infection with Aeromonas hydrophila. Dual-luciferase reporter assays and miRNA expression profiling confirmed that miR-148 targeted both CiGadd45ba and CiGadd45bb. Transfection with miR148 mimics and inhibitors altered the expression levels of proinflammatory genes, suggesting that miRNAs regulate the immune response in grass carp. Our results provide a theoretical basis for studying the molecular mechanism underlying the regulation of inflammation by miR-148 in grass carp.

2.
Artigo em Inglês | MEDLINE | ID: mdl-31957437

RESUMO

We describe herein a flexible and tunable random laser made from a flexible poly(dimethylsiloxane) substrate. The substrate is prepared by casting via soft lithography from a lotus leaf to produce a micropapilla surface structure similar to that of a lotus leaf. The micropapilla provides efficient multiple scattering for the photons generated in the gain medium, and random lasing emerges because photons undergo closed-loop paths by scattering from three equilaterally arranged micropapillae. Given the diverse distribution of microscale features on the soft substrate, the random laser spectrum can be tuned by as much as 26.0 nm by changing the pump position. Furthermore, the random laser can be easily tuned by about 14 nm by flexing it, which modifies the micropapilla density and thereby changes the reabsorption strength of the laser dye. The photostability of the random laser is ensured by sealing the gain medium (i.e., dye solution) in a closed system. The results provide a promising method to realize a variety of laser-based applications such as optical biosensors on chips, microscale structural alteration detectors, flexible wearable devices, and multicolor (even white) random lasers.

3.
Medicine (Baltimore) ; 99(4): e18951, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31977912

RESUMO

RATIONALE: An amniotic fluid embolism (AFE) is a rare, lethal syndrome that is commonly associated with disseminated intravascular coagulation (DIC). Anticoagulation therapy is the most important strategy to inhibit excessive activation of the coagulation cascade in patients with AFE and DIC. At present, treatment of AFE with rivaroxaban has not been reported. PATIENT CONCERNS: We report a 37-year-old woman (gravida 2, para 1) at 39 weeks' gestation with irregular contractions of the uterus was admitted to the obstetrical department. Ten minutes after the spontaneous rupture of the membranes, the patient complained of dyspnea and dysphoria and exhibited cyanosis of her lips. The patient's blood pressure decreased and heart rate increased rapidly, and 2100 mL of unclotted blood flowed from her vagina within 1 hour. Her platelet count dropped to 21 × 10/L, and the results from routine coagulation tests, and D-dimer and fibrin degradation product tests were obviously abnormal. DIAGNOSES: According to the current research consensus, AFE with DIC should be considered immediately when sudden cardiovascular collapse occurs around the time of labor and delivery, followed by the development of coagulopathy and hemorrhage. INTERVENTIONS: In addition, the variety of supportive treatments, rivaroxaban was used in anticoagulant therapy. OUTCOMES: At follow-up 30 and 60 days, there were no complaints of discomfort or abnormal laboratory assays. The patient recovered completely. LESSONS: This case highlights that rivaroxaban, as a direct inhibitor of activated factor Xa, demonstrates a good therapeutic efficacy for treating AFE with DIC.


Assuntos
Coagulação Intravascular Disseminada/tratamento farmacológico , Embolia Amniótica/tratamento farmacológico , Inibidores do Fator Xa/administração & dosagem , Rivaroxabana/administração & dosagem , Adulto , Transfusão de Eritrócitos , Feminino , Humanos , Gravidez , Resultado do Tratamento
4.
Artigo em Inglês | MEDLINE | ID: mdl-31916172

RESUMO

Risk assessment of pollutants to humans and ecosystems requires much toxicological data. However, experimental testing of compounds expends a large number of animals and is criticized for ethical reasons. The in silico method is playing an important role in filling the data gap. In this paper, the acute toxicity data of 1221 chemicals to Vibrio fischeri were collected. The global models obtained showed that there was a poor relationship between the toxicity data and the descriptors calculated based on linear and nonlinear regression analysis. This is due to the fact that the studied compounds contain not only non-reactive compounds but also reactive and specifically acting compounds with different modes of action (MOAs). MOAs are fundamental for the development of mechanistically based QSAR models and toxicity prediction. To investigate MOAs and develop MOA-based prediction models, the compounds were classified into baseline, less inert, reactive, and specifically acting compounds based on the modified Verhaar's classification scheme. Satisfactory models were established by multivariate linear regression (MLR) and support vector machine (SVM) analysis not only for baseline and less inert chemicals, but also for reactive and specifically acting compounds. Compared with linear models obtained by the MLR method, the nonlinear models obtained by the SVM method had better performance. The cross validation proved that all of the models were robust except for those for reactive chemicals with nN (number of nitrogen atoms) = 0 and n(C=O) (number of carbonyl groups) > 0 (Q2ext < 0.5). The application domains and outliers are discussed for those MOA-based models. The models developed in this paper are significantly helpful not only because the application domains for baseline and less inert compounds have been expended, but also the toxicity of reactive and specifically acting compounds can be successfully predicted. This work will promote understanding of toxic mechanisms and toxicity prediction for the chemicals with structural diversity, especially for reactive and specifically acting compounds.

5.
J Chin Med Assoc ; 83(1): 60-66, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31904741

RESUMO

BACKGROUND: This study aimed to investigate the expression of Th9 and Th22 cells in rats with cerebral palsy (CP) after human umbilical cord-derived mesenchymal stem cell (hUC-MSC) transplantation. METHODS: First, hUC-MSCs were isolated from fresh umbilical cords and identified. Rats were divided into the normal group, CP group, and hUC-MSC transplantation group. The Morris water maze and balance beam tests were performed to evaluate the neurobehavioral ability of the rats. The levels of TNF-α, IL-6, IL-9, and IL-22 in rat brain tissues were detected by ELISA. Th9 and Th22 proportions in brain tissues were detected by flow cytometric analysis. The mRNA levels of IL-9, IL-22, PU.1, and AHR in brain tissues were determined by qRT-PCR. RESULTS: hUC-MSC transplantation enhanced the neurobehavioral ability of CP rats. Furthermore, Th9 and Th22 proportions were decreased in brain tissues from CP rats after hUC-MSC transplantation. The levels of proinflammatory cytokines (TNF-α and IL-6), Th9-related IL-9 and PU.1, and Th22-related IL-22 and AHR were markedly higher in brain tissues from CP rats than in brain tissues from control rats, but their levels were significantly decreased after hUC-MSC transplantation. CONCLUSION: Our data indicate that Th9 and Th22 proportions are decreased in CP rats after hUC-MSC transplantation.

6.
J Mol Neurosci ; 70(1): 1-8, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31428919

RESUMO

This study presents two Chinese siblings with a rare neurodevelopmental disorder (NDD) caused by biallelic INTS1 mutations and investigates the clinical features of this disease by means of in silico analysis. Two siblings, an 11-year-old brother and a 5-year-old sister, visited our hospital due to physical retardation and profound intellectual disability. Whole-exome sequencing (WES) was performed for the girl, and Sanger sequencing was used to validate the identified variants. Phenotype correlation analysis and in silico genetic interaction network analysis were performed to investigate genes that could lead to diseases similar to the rare disease in the patients. Growth retardation, distinct intellectual disability, hypertelorism, mild cataract, uneven teeth, abnormal palmar and plantar creases, and dubious genitalia were noted in the sister. No neurological features related to neuropathy were found. The brother showed features and growth delay similar to his sister. Heterozygous novel variants of c.1645A>G,p.Met549Val and c.5881C>T,p.Gln1961* in INTS1 were considered a candidate etiology. Sanger sequencing demonstrated that the variants were inherited from the grandfather and (maternal) grandmother. Phenotype correlation analysis revealed that CTDP1 mutation-induced congenital cataracts-facial dysmorphism-neuropathy (CCFDN) mostly overlapped with the performance of our patients. In silico analysis of the genetic interaction network showed that INTS1 is highly associated with INTS8 and CTDP1. Our study further validated that biallelic INTS1 mutations could bring about the onset of a novel neurodevelopmental disorder.

7.
Metab Brain Dis ; 35(1): 83-93, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31440984

RESUMO

Post-stroke fatigue (PSF) is a common symptom after stroke and interferes with the rehabilitation. There are limited pharmacological therapies for managing PSF. Astragalus membranaceus (Huangqi) is a frequently used Chinese herbal medicine (CHM) in the treatment of fatigue in China. The aim of this review was to summarize the efficacy of adjuvant therapy with CHM Huangqi (CHM-HQ) in managing fatigue after stroke. We searched the databases in both English and Chinese for randomized controlled trials (RCTs) on CHM-HQ for PSF till November 2016. The Cochrane risk of bias tool was used to assess the quality of included trials, and the Review Manager 5.3 software was used to conduct the data analysis. Sixteen RCTs with a total of 1222 participants were included. The evidence was poor in quality with unclear or high risks of bias. Compared to routine intervention, treatment with CHM-HQ decreased the fatigue severity based on the assessment of the Fatigue Severity Scale, Fugl-Meyer and Visual Analogue Scale, and improved the quality of life as measured by the Stroke Specific Quality of Life scale, the Barthel index, and the modified Barthel index, while the adverse effects were mild. In conclusions, adjuvant therapy with CHM-HQ may benefit in managing fatigue and quality of life in stroke patients. However, stronger evidence is needed for a promising conclusion and more rigorous designs of RCTs are merited in the future.

8.
Fish Shellfish Immunol ; 97: 493-499, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31838144

RESUMO

Ctenopharyngodon idellagrowth arrest and DNA damage-inducible 45aa (CiGadd45aa) is a member of the Gadd45 family of immune-related proteins in grass carp. There is increasing evidence that microRNAs (miRNAs) are involved in the regulation of inflammatory responses and apoptosis. However, little is known about the regulatory effects of miRNAs on CiGadd45aa expression in grass carp. In the present study, CiGadd45aa was identified as a target gene of miR-731 based on miRNA expression profiling and dual-luciferase reporter assay. Our study revealed that miR-731 targets CiGadd45aa and regulates the expression of proinflammatory factors, thereby regulating immune response in grass carp. In addition, CiGadd45aa and miR-731 were both found induced apoptosis. Hence, our findings provide a theoretical basis for exploring the molecular mechanism by which miR-731 regulates inflammation and apoptosis in grass carp.

9.
Ecotoxicol Environ Saf ; 188: 109898, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31711775

RESUMO

Gamma-aminobutyric acid (GABA) plays a critical role in regulation of gonadotropin-releasing hormone (GnRH) through GABAA receptor (GABAAR). Nitric oxide (NO) production has correlation with GABA and regulates GnRH secretion. This study was performed to examine the mechanisms by which manganese (Mn) accelerate puberty onset involves GABAAR/NO pathway in the preoptic area-anterior hypothalamus (POA-AH) in immature female rats. First, female rats received daily dose of MnCl2 0 (saline), 2.5, 5 and 10 mg/kg b.w by oral gavage during postnatal day (PND) 21-32. Animals administered with 10 mg/kg MnCl2 exhibited earlier puberty onset age and advanced ovary and uterus development than these in saline-treatment group. Furthermore, we found that decrease of GABAAR result in elevated production of nitric oxide synthase1 (NOS1), NO and GnRH in the POA-AH. Second, we recorded the neuronal spikes alternation after perfusion with GABAAR inhibitor bicuculline (BIC), GABAAR agonist isoguvacine (isog), and MnCl2 from the POA-AH in acute brain slices of PND21 rats. Spontaneous firing revealed a powerful GABAAR-mediated action on immature POA-AH and confirm that MnCl2 has a significant effect on GABAAR. Third, we revealed that decrease in NOS1 and NO production by treatment with isog-alone or isog+MnCl2 contribute to the decrease of GnRH in the POA-AH and a delayed puberty onset age compared to treatment with MnCl2-alone. Together, these results suggested that excessive exposure to MnCl2 stimulates NO production through decreased GABAAR in the POA-AH to advance puberty onset in immature female rats.

10.
Talanta ; 208: 120361, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31816810

RESUMO

Routine microbial identification by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has been achieved based on the spectra of ribosomal proteins with molecular masses between 2000 and 20000Da. It is a rapid, cost-effective, and simple method to characterize different species of microorganisms. But for some subspecies of molds, there are high similarities between their spectra in 2000-20000Da, it makes them indistinguishable in this mass range. Based on the specialized metabolite production, there are obvious differences between the high resolution spectra of the same samples in 600-2000Da. It allows the rapid discrimination of these microbial subspecies. The ability of the method to discriminate microbial subspecies was demonstrated by characterizing three different Aspergillus niger strains. Furthermore, this approach has been applied to discriminate two different Acremonium alternatum strains which were collected from mildew plants. It demonstrated the applicability of the method to the actual samples. The high resolution MS in the range of 600-2000Da was presented as a complementary approach for the routine method in 2000-20000Da. The molds could be identified into species-level group by the spectra between 2000 and 20000Da and the strains within each group could be further discriminated based on differences in metabolites. The spectra between 2000 and 20000Da and the spectra between 600 and 2000Da were obtained from the same samples, which extracted with the same method. There is no need of additional pre-processing to obtain the high resolution spectra. It potentially provides a powerful tool for the fast and accurate identification of microbial subspecies.

11.
Immunity ; 52(1): 109-122.e6, 2020 01 14.
Artigo em Inglês | MEDLINE | ID: mdl-31882361

RESUMO

Recent work suggests that cholesterol metabolism impacts innate immune responses against infection. However, the key enzymes or the natural products and mechanisms involved are not well elucidated. Here, we have shown that upon DNA and RNA viral infection, macrophages reduced 7-dehydrocholesterol reductase (DHCR7) expression. DHCR7 deficiency or treatment with the natural product 7-dehydrocholesterol (7-DHC) could specifically promote phosphorylation of IRF3 (not TBK1) and enhance type I interferon (IFN-I) production in macrophages. We further elucidated that viral infection or 7-DHC treatment enhanced AKT3 expression and activation. AKT3 directly bound and phosphorylated IRF3 at Ser385, together with TBK1-induced phosphorylation of IRF3 Ser386, to achieve IRF3 dimerization. Deletion of DHCR7 and the DHCR7 inhibitors including AY9944 and the chemotherapy drug tamoxifen promoted clearance of Zika virus and multiple viruses in vitro or in vivo. Taken together, we propose that the DHCR7 inhibitors and 7-DHC are potential therapeutics against emerging or highly pathogenic viruses.


Assuntos
Desidrocolesteróis/metabolismo , Fator Regulador 3 de Interferon/metabolismo , Interferon Tipo I/biossíntese , Macrófagos/imunologia , Oxirredutases atuantes sobre Doadores de Grupo CH-CH/metabolismo , Estomatite Vesicular/imunologia , Células A549 , Animais , Linhagem Celular , Colesterol/metabolismo , Ativação Enzimática/imunologia , Células HEK293 , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Oxirredutases atuantes sobre Doadores de Grupo CH-CH/antagonistas & inibidores , Oxirredutases atuantes sobre Doadores de Grupo CH-CH/genética , Fosfatidilinositol 3-Quinases/metabolismo , Fosforilação , Proteínas Proto-Oncogênicas c-akt/metabolismo , Células RAW 264.7 , Interferência de RNA , RNA Interferente Pequeno/genética , Vírus da Estomatite Vesicular Indiana/imunologia
12.
Artigo em Inglês | MEDLINE | ID: mdl-31794883

RESUMO

c-Jun N-terminal kinases (JNKs) and p38s are central components of signal transduction pathways, which are stimulated mainly by environmental stress and inflammatory cytokines. Manipulation of JNK and p38 dependent immune responses either boosts or subdues immune responses to infectious diseases or inflammatory disorders. In this study, we analyzed the whole-genome database of the grass carp and identified 4 JNK and 6 p38 genes. JNK and p38 genes of grass carp were distributed in 7 out of 24 chromosomes. All JNK and p38 proteins contained characteristic dual-phosphorylation site. The JNKs contain a specific dual-phosphorylation consensus ((Thr-Pro-Tyr) that is different from that of the p38 proteins (Thr-Gly-Tyr). Deduced gene secondary structure analyses as well as the syntenic analyses further supported their annotation and orthologs. Results of tissue distribution detection revealed that JNK and p38 genes exhibited lower expression in health grass carp. The mRNA expression levels of JNK and p38 genes were significantly up-regulated in tissues and CIK cells after bacterial infection, indicating their potential roles in bacterial-regulated immune responses. These findings in our study will facilitate the further evolutionary characterization of JNK and p38 genes in teleost species and provide a theoretical basis for their functional study.

13.
Fish Shellfish Immunol ; 98: 34-44, 2019 Dec 25.
Artigo em Inglês | MEDLINE | ID: mdl-31883472

RESUMO

Ctenopharyngodon idella growth arrest and DNA damage-inducible 45 ab (CiGadd45ab) is a subtype of the Gadd45a gene of the Gadd45 family in grass carp. There is increasing evidence that microRNAs (miRNAs) are involved in the regulation of inflammatory and apoptotic responses. However, little is known about the regulatory effects of miRNAs on CiGadd45ab expression. In the present study, CiGadd45ab was identified as a target gene of miR-23a-3p and miR-23a-5p, based on miRNA expression profiling and a dual-luciferase reporter assay. In addition, miR-23a-3p and miR-23a-5p were both confirmed to be involved in the inflammatory response following infection with Aeromonas hydrophila by targeting CiGadd45ab. Transfection with miR-23a-3p and miR-23a-5p mimics and inhibitor altered proinflammatory gene expression and apoptosis rate, thereby suggesting that miRNAs regulate immune response and anti-apoptosis by targeting CiGadd45ab in grass carp. Our results provide a theoretical basis for exploring the molecular mechanisms by which miR-23a-3p and miR-23a-5p target CiGadd45ab to regulate inflammation and apoptosis against bacterial infection in grass carp.

14.
J Clin Invest ; 129(12): 5343-5356, 2019 Dec 02.
Artigo em Inglês | MEDLINE | ID: mdl-31682236

RESUMO

Both miRNAs and A-to-I RNA editing, a widespread nucleotide modification mechanism, have recently emerged as key players in cancer pathophysiology. However, the functional impact of RNA editing of miRNAs in cancer remains largely unexplored. Here, we focused on an ADAR2-catalyzed RNA editing site within the miR-379-5p seed region. This site was under-edited in tumors relative to normal tissues, with a high editing level being correlated with better patient survival times across cancer types. We demonstrated that in contrast to wild-type miRNA, edited miR-379-5p inhibited cell proliferation and promoted apoptosis in diverse tumor contexts in vitro, which was due to the ability of edited but not wild-type miR-379-5p to target CD97. Importantly, through nanoliposomal delivery, edited miR-379-5p mimics significantly inhibited tumor growth and extended survival of mice. Our study indicates a role of RNA editing in diversifying miRNA function during cancer progression and highlights the translational potential of edited miRNAs as a new class of cancer therapeutics.

15.
BMC Cancer ; 19(1): 1061, 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31703584

RESUMO

BACKGROUND: To reveal roles of reactive oxygen species (ROS) status in chemotherapy resistance and to develop a ROS scoring system for prognosis prediction in ovarian cancer. METHODS: We tested the sensitizing effects of ROS elevating drugs to cisplatin (cDDP) in ovarian cancer both in vitro and in vivo. A ROS scoring system was developed using The Cancer Genome Atlas (TCGA) database of ovarian cancer. The associations between ROS scores and overall survival (OS) were analyzed in TCGA, Tothill dataset, and our in-house dataset (TJ dataset). RESULTS: ROS-inducing drugs increased cisplatin-induced ovarian cancer cell injury in vitro and in vivo. ROS scoring system was established using 25 ROS-related genes. Patients were divided into low (scores 0-12) and high (scores 13-25) score groups. Improved patient survival was associated with higher scores (TCGA dataset hazard ratio (HR) = 0.43, P < 0.001; Tothill dataset HR = 0.65, P = 0.022; TJ dataset HR = 0.40, P = 0.003). The score was also significantly associated with OS in multiple datasets (TCGA dataset r2 = 0.574, P = 0.032; Thothill dataset r2 = 0.266, P = 0.049; TJ dataset r2 = 0.632, P = 0.001) and with cisplatin sensitivity in ovarian cancer cell lines (r2 = 0.799, P = 0.016) when used as a continuous variable. The scoring system showed better prognostic performance than other clinical factors by receiver operating characteristic (ROC) curves (TCGA dataset area under the curve (AUC) = 0.71 v.s. 0.65, Tothill dataset AUC = 0.73 v.s. 0.67, TJ dataset AUC = 0.74 v.s. 0.66). CONCLUSIONS: ROS status is associated with chemotherapy resistance. ROS score system might be a prognostic biomarker in predicting the survival benefit from ovarian cancer patients.

16.
Oncogene ; 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31705064

RESUMO

Ovarian cancer selective metastasizes to the omentum contributing to the poor prognosis associated with ovarian cancer. However, the mechanism underlining this propensity and therapeutic approaches to counter this process has not been fully elucidated. Here, we show that MCP-1 produced by omental adipocytes binding to its cognate receptor CCR-2 on ovarian cancer cells facilitates migration and omental metastasis by activating the PI3K/AKT/mTOR pathway and its downstream effectors HIF-1α and VEGF-A in cell lines, xenografts, and transgenic murine models. MCP-1 antibody significantly decreased tumor burden and increased survival of mice in vivo. Interestingly, metformin decreased omental metastasis at least partially by inhibiting MCP-1 secretion from adipocytes independent of direct effects on cancer cells. Together this suggests a novel target of MCP-1/CCR-2 axis that could benefit ovarian cancer patients.

17.
Curr Med Sci ; 39(5): 816-819, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31612401

RESUMO

Sometimes endometrial polyps, submucosal myomas, and endometrial cancer show similar findings under ultrasonography. The aim of this study was to assess the antidiastole value of blood flow parameters using three-dimensional (3D) power Doppler ultrasonography angiography (PDA) between endometrial cancer and uterine parenchyma lumps. The data of the blood flow indices in 3D-PDA including the vascularization index (VI), flow index (FI), and vascularization flow index (VFI) in 40 patients with endometrial cancer and 41 patients with uterine parenchyma lumps (endometrial polyps and submucosal myomas) were retrospectively analysed and compared utilizing Virtual Organ Computer-aided AnaLysis (VOCAL) software. The results showed that all the blood flow parameters (VI, FI, VFI) were significantly higher in women with endometrial cancer than in those with uterine parenchyma lumps (P<0.001). The area under the curve of ROC of VI, FI, and VFI was 0.98, 0.84, and 0.97, respectively. Thus, the best predictor of endometrial carcinoma was VI with a sensitivity of 97.0% and a specificity of 91.0%. The optimal cutoff value of VI was 4.06%. Our data demonstrated that all of the blood flow signal parameters (including VI, FI, and VFI) in 3D power Doppler ultrasonography had significant antidiastole values between endometrial cancer and uterine parenchyma lumps to assist clinicians in properly diagnosing patients.

18.
Minerva Anestesiol ; 85(12): 1273-1280, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31486620

RESUMO

BACKGROUND: The efficacy of an ultrasound guided transmuscular quadratus lumborum block (QLB) for perioperative analgesia of the upper and lower abdomen remain debatable. The purpose of this study was to compare the cutaneous sensory blocked area (CSBA) between QLB blocks performed at the L2 vs. L4 levels. METHODS: Twenty-two healthy volunteers were randomized 1:1 to receive an ultrasound guided right transmuscular QLB at the L2 level (group QL2) or L4 level (group QL4). A cold stimulus was applied for testing of the CSBA at 30 minutes after the blockade was performed. The CSBA was mapped and then calculated. Three hours after the QLB, a cold stimulus was applied once every hour until sensation returned normal and the effective block duration for each volunteer was determined and recorded. RESULTS: The maximum cephalad dermatome level reached was T7 in group QL2 vs. T11 in group QL4, respectively. Caudally, both groups reached the L2 dermatome level. The QL2 block primarily affected dermatomes T9 to L1, while the QL4 block affected T11 to L1. The total CSBA was larger in QL2 group than that in QL4 group (748 [171] cm2 vs. 501 [186] cm2, P=0.004). The effective duration of the QLB was significantly longer in group QL2 than in group QL4 (18.5 [2.0]h vs. 14.1 [4.7]h, P=0.012). The number of affected dermatomes assessed by cold test was significantly larger for the volunteers in groups QL2 (4.6 [0.81] vs. 2.1 [0.30], P<0.001). CONCLUSIONS: Ultrasound guided transmuscular QLB injection of 0.375% 20 mL ropivacaine at the L2 level produced a widespread cutaneous sensory blockade and a prolonged sensory block to cold sensation compared with the L4 level.

19.
Fish Shellfish Immunol ; 94: 258-263, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31513913

RESUMO

Grass carp septicemia is a systemic inflammatory response that develops following a bacterial infection. The hyperinflammatory state develops could lead to septic shock and lethality. There is increasing evidence that microRNAs are involved in the regulation of the inflammatory response. In the present study, miR-21 was confirmed to be involved in the inflammatory response following infection with Aeromonas hydrophila and LPS stimulation. Both jnk and ccr7 were identified as target gene of miR-21 by overexpression, inhibition, and dual luciferase reporter assays experiments. Meanwhile, miR-21 targets the jnk and ccr7 to modulate downstream pro-inflammatory factors tnf-α, il-1ß, il-6, and il-12. Our results provide a theoretical basis for exploring the molecular mechanism of grass carp miR-21 regulating inflammation.

20.
Fish Shellfish Immunol ; 94: 220-229, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31494279

RESUMO

Myeloid differentiation factor 88 (MyD88) is an important transduction protein in the Toll-like receptor signaling pathway. In this study, we identified the cDNA of the MpMyD88 gene in black carp. We found that MpMyD88 was widely distributed in the tissues tested and showed significant immune responses both in vitro and in vivo after stimulation with bacterial and pathogen-associated molecular patterns. After MpMyD88 overexpression/silencing, proinflame-matory cytokines (TNF-α, IFN-α, IL-6, and IL-8) also showed significant up-regulation/down-regulation. Moreover, we found that the antibacterial ability of cells over-expressing MpMyD88 was significantly stronger than that of control cells, while that of silenced MpMyD88 was significantly lower than that in control cells. Besides, we found that the overexpression of MpMyD88 significantly increased the activity of NF-κB. These results indicate that MpMyD88 plays an important role in the innate immune response.

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