Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 94
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Artigo em Inglês | MEDLINE | ID: mdl-34628047

RESUMO

As a common aquatic pathogen, Vibrio parahaemolyticus can cause a variety of diseases of shrimp, especially acute hepatopancreatic necrosis disease (AHPND), which leads to great losses to the aquaculture industry around the world. However, the molecular mechanism of V. parahaemolyticus infection is still unclear. Neocaridina denticulate sinensis is a kind of small ornamental shrimp that is popular in aquarium trade, and due to its tenacious vitality, rapid growth, high reproductive capacity, it is very suitable to be developed as an animal model for basic research on decapod crustaceans. Thus, in this paper, transcriptomes of N. denticulate sinensis hepatopancreas with or without V. parahaemolyticus injection were explored. The results showed that a total of 23,624 genes with the N50 of 2705 bp were obtained. Comparative transcriptomic analysis revealed 21,464 differentially expressed genes between the V. parahaemolyticus infected and non-infected group, of which, 11,127 genes were up-regulated and 10,337 genes were down-regulated. Functional enrichment analysis suggested that many DEGs enriched in immune related pathways, including MAPK signaling pathway, Phosphatidylinositol signaling system, Chemokine signaling pathway, Phagosome and Jak-STAT signaling pathway and so on. Eight genes were selected randomly for qRT-PCR to verify the transcriptome sequencing results and the results showed the expression of these genes were consistent with the transcriptome results. Our work provides a unique and important dataset that contributes to the understanding of the molecular mechanisms of the immune response to V. parahaemolyticus infection and may further provide the basis for the prevention and resolution of bacterial diseases.

2.
Small ; 17(47): e2103877, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34636168

RESUMO

DNA origami has been widely used as a modular platform for condensation of functional molecules to assemble optical, electronic, and biological components. However, the heterogeneous condensation with greater diversities in chemical composition templated with DNA origami is still challenging. Herein, a programmable deposition method is developed to precisely condense silver-silica nanohybrids on DNA origami templates. First, the site-specific metallization of Ag is achieved by thiol group-initiated silver reduction at the designed areas of DNA origami. Next, cysteamine is used to selectively modify the condensed Ag surface with positively charged amino groups for creating an electronically different environment for site-specific placement of silica by a modified Stöber method. Using these strategies, customized patterning of both silver and silica on tubular and rectangular DNA origami nanostructures is successfully achieved with nanoscale spatial resolution. These findings will greatly facilitate the development of DNA nanotechnology-based bottom-up nanofabrication.

3.
Front Oncol ; 11: 690777, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34381715

RESUMO

To estimate whether adjuvant radiotherapy is necessary for patients with stage IA1-IIA1 cervical cancer after laparoscopic hysterectomy, 221 patients were retrospectively analyzed. Sixty-two of them were treated with laparoscopic hysterectomy and adjuvant radiotherapy (group A), 115 underwent open surgery (group B) and 44 received laparoscopic hysterectomy alone (group C). Results showed that the 3-year local recurrence-free survival (LRFS) rates of group A, B and C were 98.4%, 97.4% and 86.4%, respectively. The LRFS rates of group A and B surpassed C (A vs. B, p=0.634; A vs. C, p=0.011; B vs. C, p=0.006). The inter-group differences of 3-year overall survival (OS) and distant metastasis free survival (DMFS) were not statistically significant. In subgroup analysis of stage IB disease, the 3-year LRFS rates of group A, B and C were 100%, 98.8% and 83.1%, the 3-year OS rates of group A, B and C were 100%, 98.9% and 91.5%, respectively. The 3-year LRFS and OS rates of group A and B were significantly superior to group C (p<0.05). Our findings suggest that adjuvant radiotherapy can reduce the risk of recurrence for women with early-stage cervical cancer after laparoscopic hysterectomy and bring survival benefits for patients with stage IB disease.

4.
Ultrasound Med Biol ; 47(11): 3253-3262, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34400032

RESUMO

The aim of this study was to evaluate blood perfusion of the renal cortex during ischemia-reperfusion (I/R) injury using quantitative contrast-enhanced ultrasound (CEUS) parameters. In this experiment, 24 rabbits were randomly divided into four groups (N = 6): sham-operated group, 24-h post-operation for I/R injury group (24-h I/R), 3-d post-operation for I/R injury group (3-d I/R) and 5 d post-operation for I/R injury group (5 d I/R). All quantitative CEUS parameters were monitored and included the gradient from the start frame to the peak frame (Grad), area under the curve (Area), time-to-peak (TTP), difference between B(intercept intensity at t=0) and A(the intensity attenuation t= 0) and arrival time (AT). Subsequently, we analyzed the changes in these parameters, as well as the correlation between changes in CEUS parameters and pathological parameters. AT and TTP values peaked 3 d after I/R surgery, which correlated with the most significant pathological changes at the same time point. These parameters (such as AT, TTP and Grad) may be useful in dynamically monitoring the severity of tissue damage at the early stage of I/R injury.

5.
Cancer Lett ; 517: 105, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34274168

RESUMO

This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal). This article has been retracted at the request of the Editor-in-Chief. Upon investigation, it was discovered that 4 out of 8 images contain fabrication. Fig. 6D, E, 8E and 7F have used the same microscopic image for different experimental settings. The Figure represented in 8E is the correct image placement. The scientific community takes a very strong view on this matter and apologies are offered to readers of the journal that this was not detected during the submission process.

6.
Am J Med Genet A ; 185(10): 3074-3082, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34047005

RESUMO

PSTPIP1-associated myeloid-related proteinemia inflammatory (PAMI) syndrome caused by mutations in PSTPIP1 is a rare inflammatory disorder that can be easily misdiagnosed. It is characterized by anemia, arthritis, cutaneous inflammation, recurrent infections, growth failure, hepatosplenomegaly, lymphadenopathy, hyperzincemia/hypercalprotectinemia, neutropenia, thrombocytopenia, and elevated inflammatory indicators. This study describes the cases of two pediatric female patients with long-standing recurrent arthralgia in different parts of the extremities and severe anemia, respectively, who were misdiagnosed and treated for aseptic necrosis of the femoral head and severe autoimmune hemolytic anemia, respectively. High-throughput sequencing analysis revealed a de novo heterozygous missense mutation (c.748G > A, p. Glu250Lys) in exon 11 of PSTPIP1 (NM_003978.5) in both patients, which supported a diagnosis of PAMI. The patients were treated with prednisone and etanercept, which improved their symptoms, but neutropenia remained unchanged. These cases highlight the importance of genetic assessment for the accurate diagnosis of PAMI and to ensure adequate and timely treatment of these patients.

7.
Animals (Basel) ; 11(4)2021 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-33920798

RESUMO

The objective of this study was to evaluate the efficacy of supplementing a corn-wheat-soybean meal-based diet with a multicarbohydrase and phytase complex (MCPC) on growth performance, apparent total tract digestibility (ATTD) of nutrients, carcass traits, and meat quality in growing-finishing pigs. A total of 300 pigs (Duroc × Large White × Landrace; body weight = 25.3 ± 0.7 kg) were randomly allotted to three groups with 10 replicates of 10 pigs each. Pigs from three groups were fed positive control (PC) or negative control (NC), without or with MCPC diets, respectively. The MCPC supplied at least 1800, 1244, 6600, and 1000 units of xylanase, ß-glucanase, α-arabinofuranosidase, and phytase per kilogram of diet, respectively. The NC diet was the PC diet but reduced in net energy (NE), digestible amino acids (dig. AA), digestible P (dig. P), and Ca by 74 kcal/kg, 7.0%, 0.134, and 0.119 percentage points, respectively. The diets were fed in 4 growth phases based on body weight (BW): phase 1: 25-50 kg, phase 2: 50-75 kg, phase 3: 75-100 kg, and phase 4: 100-135 kg. Compared to the PC, the NC diet decreased (p < 0.05) body weight gain, feed intake, and(or) feed to gain ratio during the growing/finishing phases 1, 2, 3, and 4. It also reduced (p < 0.05) the ATTD of crude protein, crude fat, P, and Ca of pigs. MCPC supplementation improved (p < 0.05) the body weight gain, feed intake, and(or) feed to gain ratio in phases 2, 3, and 4 and the ATTD of crude protein, crude fat, ash, P, and Ca for the NC diet. Additionally, dietary treatment had no effects on carcass traits and meat quality with the exception that the loin eye area in the NC plus MCPC diet was higher (p < 0.05) than the NC diet. In conclusion, the addition of MCPC to a corn-soybean meal-wheat-based diet reduced in energy and nutrients improved the growth performance and nutrient digestibility but had little effect on carcass traits and meat quality in growing-finishing pigs.

8.
PeerJ ; 9: e10932, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33643717

RESUMO

The AT-hook motif nuclear-localized (AHL) family is a plant transcription factor family, which plays an important role in growth and development and stress responses. We identified and analyzed 37 AHL genes in poplar (Populus trichocarpa). Phylogenetic analysis classified the PtrAHL members into three subfamilies based on their conserved domain. All PtrAHL paralogous pairs evolved under purifying selection. The promoter analysis revealed the presence of stress-related and phytohormone-related cis-elements of the PtrAHL genes. Our analysis of the tissue-specific expression pattern of PtrAHL genes indicated their significance in tissue and organ development. Network-based prediction suggested that PtrAHL genes may interact with histone deacetylases (HDAC) and participate in the development of organs, such as roots. Drought negatively impacts plant growth and development. ABA is produced under osmotic stress condition, and it takes an important part in the stress response and tolerance of plants. Real-time quantitative PCR (qRT-PCR) showed that PtrAHL genes were induced by drought stress and ABA treatment. These insights into the expression of PtrAHL genes under stress provide a basis for PtrAHL gene functional analysis. Our study will help develop new breeding strategies to improve drought tolerance in poplar.

9.
Chempluschem ; 86(2): 284-290, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33605561

RESUMO

While the protein assemblies have been found widely existing and playing significant roles in biological systems, their imitation and re-construction is further boosting more applications in biomedical research, such as enzymatic reaction regulation, sensing, and biomedicine. DNA nanotechnology provides a programmable strategy for the fabrication of nanostructures with unprecedented accuracy on the nanoscale. By linking the DNA nanotechnology with proteins of different functions, the precise construction of DNA-guided protein assemblies can be achieved for various biomedical applications. This minireview summarizes the recent advances in the programmable protein assemblies on DNA nanoplatforms and discusses the outlook of DNA-guided protein assemblies in the biomedical research.


Assuntos
Pesquisa Biomédica , DNA/química , Proteínas/síntese química , Animais , Humanos , Nanoestruturas , Proteínas/química
10.
Front Immunol ; 11: 558712, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33101273

RESUMO

Sepsis/endotoxemia activates the NLRP3 inflammasome of macrophages leading to the maturation and release of IL-1ß, an important mediator of the inflammatory response. Reactive oxygen species have been implicated in NLRP3 inflammasome activation. Further, our preliminary studies indicated that LPS challenge of cardiac fibroblasts could phosphorylate protein kinase R (PKR) on threonine 451 and increase message for pro-IL-1 ß. Thus, the major aim of the present study was to address the role of PKR and the oxidant, peroxynitrite, in the two-tiered function of the NLRP3 inflammasome (priming and activation). Materials and Methods: Isolated murine fibroblasts were primed with LPS (1 µg/ml) for 6 h and subsequently activated by an ATP (3 mM) challenge for 30 min to induce optimum functioning of the inflammasome. Increased levels of NLRP3 and pro-IL-1ß protein (Western) were used as readouts for inflammasome priming, while activation of caspase 1 (p20) (Western) and secretion of IL-1ß (ELISA) were indicative of inflammasome activation. Results: Inhibition of PKR (PKR inhibitor or siRNA) prior to priming with LPS prevented the LPS-induced increase in NLRP3 and pro-IL-1ß expression. Further, inhibition of PKR after priming, but before activation, did not affect NLRP3 or pro-IL-1ß protein levels, but markedly reduced the activation of caspase 1 and secretion of mature IL-1ß. In a similar fashion, a peroxynitrite decomposition catalyst (Fe-TPPS) prevented both the priming and activation of the NLRP3 inflammasome. Finally, pretreatment of the fibroblasts with Fe-TPPS prevented the LPS-induced PKR phosphorylation (T451). Conclusion: Our results indicate that peroxynitrite-/PKR pathway modulates priming and activation of NLRP3 inflammasome in LPS/ATP challenged cardiac fibroblasts.


Assuntos
Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Inflamassomos/metabolismo , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Ácido Peroxinitroso/farmacologia , eIF-2 Quinase/metabolismo , Animais , Camundongos , Modelos Biológicos , Fosforilação , Transdução de Sinais/efeitos dos fármacos
11.
Huan Jing Ke Xue ; 41(10): 4382-4391, 2020 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-33124370

RESUMO

Based on the online monitoring data of gaseous pollutants and components in PM2.5 from Chengdu super observatory of atmospheric environment, the meteorological factors and component characteristics of three haze pollution process in Chengdu from 2019 to 2020 were analyzed. The CMB model was adopted to simulate the sources and variation trends of PM2.5 pollution during the study period, and the causes of each pollution process were analyzed. The results showed that all the three pollution processes occurred under adverse meteorological conditions, where the relative humidity and temperature continued to rise and the wind speed and boundary layer height continued to decrease. The average daily relative humidity was greater than 70%, average daily temperature was greater than 8℃, average daily wind speed was less than 0.8 m ·s-1, and average daily boundary layer height was less than 650 m. During the three events of pollution, the main components were NO3-, OC, NH4+, and SO42-. Among them, the mass concentration and proportion of NO3- increased by 1.47-2.09 and 0.22-0.35 times, respectively, during the pollution period as compared to those during the clean period. NO3- was a key component of PM2.5 pollution during winter in Chengdu. During the three pollution processes, the mean values of SOR and NOR were 0.40 and 0.27, respectively, and the secondary transformation degree of SO2 and NOx was high. The conversion of SO2 to SO42- was mainly dominated by heterogeneous oxidation at night, and the conversion of NOx to NO3- was dominated by heterogeneous hydrolysis. The characteristics of the three processes were slightly different. Process Ⅰ showed evident secondary nitrate-dominated characteristics. During the period of rising PM2.5 concentration in process Ⅱ, it was mainly affected by coal emissions, but during the periods of high PM2.5 concentration, it was mainly affected by NO3-. Process Ⅲ was also a nitrate-dominated process, but emissions of fossil fuel combustion had increased during certain polluted periods. Secondary nitrate, secondary sulfate, motor vehicles, and coal combustion were the main pollution sources during the study period. The PM2.5 concentration was positively correlated with the contribution of secondary nitrate and negatively correlated with the contribution of dust source.


Assuntos
Poluentes Atmosféricos , Poluição do Ar , Aerossóis/análise , Poluentes Atmosféricos/análise , Poluição do Ar/análise , China , Monitoramento Ambiental , Material Particulado/análise , Estações do Ano
12.
AMB Express ; 10(1): 160, 2020 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-32880769

RESUMO

Cytotoxicity of CuO nanoparticles (NPs) are an impediment in utilizing them as an effective nanocarriers of chemotherapeutic drugs for targeted drug delivery in nasopharyngeal cancer. In our current study, we have designed a two-step synthesis and coating of CuO NPs with different concentrations of PLGA (polylactide-co-glycolide) to reduce the cytotoxicity. This was further conjugated with folic acid to enhance targeting to specific tissue. The multiple drugs loaded in the NPs were two potent anticancer drugs doxorubicin and docetaxel. A complete characterization studies including micrographic analysis, zeta potential measurements, polydispersity index, Fourier transform infrared spectroscopy (FTIR), encapsulation and loading efficiencies, stability and in vitro release studies were done. Cytoxicity studies were done with MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, acridine orange/ethidium bromide and DAPI (4, 6-diamidino-2-phenylindole, dihydrochloride) staining procedures. Impediametric studies were also carried out to reinforce the reduction in cytotoxicity. Finally the cellular uptake of the NPs was seen. It was evident from the results that the multiple drugs loaded CuO NPs formed with PLGA coating were uniform, non-agglomerated in size ranging from 180 to 195 nm. The FTIR revealed no major changes in drug peaks. Encapsulation and loading efficiencies showed sufficient amount of drug being loaded into the NPs. The drug loaded NPs showed no change in size or zeta potential even after a period of 30 days. The cytotoxicity studies revealed significant reduction in toxicity after coating the surface treated with PLGA as evident from the microscopic analysis of cells. Hence the current study may be prioritized and further in vivo/in vitro studies may be carried out.

13.
Rev Sci Instrum ; 91(6): 064701, 2020 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-32611026

RESUMO

In this paper, a single-well model based on the piecewise function and classical bistable stochastic resonance (CBSR) is proposed. The steady state probability density of particles and mean first passage time in the model are calculated. The output characteristics and performance of the proposed model are analyzed through numerical simulation. On the basis of CBSR and the proposed model, an adaptive system is established (ACSSR) to generate the highest gain of signal-to-noise ratio (SNRg). Finally, the effectiveness of ACSSR in weak signal detection is verified with both simulated and experimental input signals. The results indicate that the ACSSR could detect the defect signal correctly and improve the SNRg.

14.
Angew Chem Int Ed Engl ; 59(40): 17697-17704, 2020 09 28.
Artigo em Inglês | MEDLINE | ID: mdl-32573062

RESUMO

Artificial nanorobots that can recognize molecular triggers and respond with programable operations provide an inspiring proof-of-principle for personalized theragnostic applications. We have constructed an intelligent DNA nanorobot for autonomous blood anticoagulation in human plasma. The DNA nanorobot comprises a barrel-shaped DNA nanostructure as the framework and molecular reaction cascades embedded as the computing core. This nanorobot can intelligently sense the concentration of thrombin in the local environment and trigger an autonomous anticoagulation when excess thrombin is present. The triggering concentration of thrombin at which the nanorobot responds can be tuned arbitrarily to avoid possible side effects induced by excess thrombin. This makes the nanorobot useful for autonomous anticoagulation in various medical scenarios and inspires a more efficient and safer strategy for future personalized medicine.


Assuntos
Anticoagulantes/química , DNA/química , Nanoestruturas/química , Trombina/química , Anticoagulantes/metabolismo , Sítios de Ligação , DNA de Cadeia Simples/química , Transferência Ressonante de Energia de Fluorescência , Humanos , Simulação de Acoplamento Molecular , Trombina/metabolismo
15.
Nat Nanotechnol ; 15(8): 709-715, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32451504

RESUMO

Early and precise cancer diagnosis substantially improves patient survival. Recent work has revealed that the levels of multiple microRNAs in serum are informative as biomarkers for the diagnosis of cancers. Here, we designed a DNA molecular computation platform for the analysis of miRNA profiles in clinical serum samples. A computational classifier is first trained in silico using miRNA profiles from The Cancer Genome Atlas. This is followed by a computationally powerful but simple molecular implementation scheme using DNA, as well as an effective in situ amplification and transformation method for miRNA enrichment in serum without perturbing the original variety and quantity information. We successfully achieved rapid and accurate cancer diagnosis using clinical serum samples from 22 healthy people (8) and people with lung cancer (14) with an accuracy of 86.4%. We envision that this DNA computational platform will inspire more clinical applications towards inexpensive, non-invasive and rapid disease screening, classification and progress monitoring.


Assuntos
Computadores Moleculares , Detecção Precoce de Câncer/métodos , Perfilação da Expressão Gênica/métodos , Neoplasias Pulmonares/diagnóstico , Biomarcadores Tumorais/sangue , Biomarcadores Tumorais/genética , Biologia Computacional , Humanos , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/metabolismo , MicroRNAs/sangue , MicroRNAs/genética
16.
Mar Pollut Bull ; 154: 111049, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32174499

RESUMO

The Bohai Sea is well-known as a source of atmospheric methane (CH4). However, the main regulate factors of the spatiotemporal distribution of CH4 and its sea-to-air flux remain largely unknown. In this study, the observed CH4 concentration ranged from 4.8 to 32.7 nmol/L and 3.1 to 15.2 nmol/L in August and November of 2014, respectively. The main factors that influence the distribution of CH4 and its sea-to-air flux were stratification, solubility, and current structure for the mid-west depression basins, the permanent well-mixed seawater column and CH4 source strength for the centre shallow ridge zone, and the upwelling for the east depression basin, respectively. Meanwhile, wind also plays an important role in sea-to-air CH4 flux in the study area except the centre shallow ridge zone. Upwelling made the east depression basin the most intensive source of CH4, with a flux of 2 to 4 times higher than the other sub-regions.


Assuntos
Metano , Água do Mar , China , Estações do Ano , Vento
17.
Chemosphere ; 251: 126412, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32171132

RESUMO

To date, the impact of air-sea exchange on spatial distribution of atmospheric methane (CH4 hereafter) remains less understood in the coastal areas of China. Here we measured the dissolved and atmospheric CH4 in the Dalian Bay and adjacent area in July and August 2014, respectively. Results showed that the study area was a net significant source of atmospheric CH4, with a mean sea-to-air CH4 flux of 170.6 ± 149.5 µmol/(m2·day). We optimized a method to accurately quantify the elevated atmospheric CH4 mole fraction (△CH4 hereafter) caused by air-sea exchange. The calculated △CH4 in the study area ranged from 15.4 to 102.1 nmol/mol, 1.5-10.2 nmol/mol, and 0.03-0.22 nmol/mol at the mixing height of 1, 10, and 471 m, respectively. The △CH4 mole fractions caused by air-sea exchange were positive with sea-to-air CH4 flux and in situ observed atmospheric CH4 mole fraction, while negative with altitude. Under the standard conditions, we defined 50.8 µmol/(m2·day) as the criteria value of sea-to-air CH4 flux which could result in a detectable elevation atmospheric CH4 mole fraction at a height of 10 m.


Assuntos
Poluentes Atmosféricos/análise , Monitoramento Ambiental/métodos , Metano/análise , Baías/química , China , Estações do Ano
18.
Top Curr Chem (Cham) ; 378(2): 31, 2020 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-32146596

RESUMO

DNA nanotechnology, based on sequence-specific DNA recognition, could allow programmed self-assembly of sophisticated nanostructures with molecular precision. Extension of this technique to the preparation of broader types of nanomaterials would significantly improve nanofabrication technique to lower nanometer scale and even achieve single molecule operation. Using such exquisite DNA nanostructures as templates, chemical synthesis of polymer and inorganic nanomaterials could also be programmed with unprecedented accuracy and flexibility. This review summarizes recent advances in the synthesis and assembly of polymer and inorganic nanomaterials using DNA nanostructures as templates, and discusses the current challenges and future outlook of DNA templated nanotechnology.


Assuntos
DNA/química , Compostos Inorgânicos/química , Nanoestruturas/química , Nanotecnologia/métodos , Polímeros/química , Animais , Lipossomos/química , Polímeros/síntese química
19.
J Med Virol ; 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-31994744

RESUMO

Sequential immunization with antigens from different strains of HIV-1, influenza viruses or dengue viruses induced cross-neutralizing antibodies and enhanced the antibody responses against previous antigens. The characteristics of neutralizing antibodies induced by sequential immunization with different types of human papillomavirus (HPV) L1 virus-like particles (L1VLPs) are unclear. In this study, mice were primed with one or two types (HPV-16 or HPV16/18) of L1VLPs, then boosted sequentially with HPV6/18/45/11/31/58 or HPV6/45/11/31/58 L1VLPs, and sera were analyzed with HPV pseudovirus-based neutralization assay. The results showed that neutralizing activities against earlier immunized vaccine types were enhanced gradually by subsequent immunizations, and low levels of neutralizing activities against nonvaccine types (HPV33/35/52/59/68) were also observed. After absorbing the immune sera with vaccine-type (HPV16/18/45) L1VLPs, neutralizing activities against tested priming and boosting types (HPV16/18/58) decreased significantly, and that against nonvaccine type (HPV-33) was also partially eliminated. Moreover, neutralizing activities against vaccine types (HPV16/58) were significantly reduced after absorbing with nonvaccine-type VLPs (HPV33/52). These data suggest that cross-neutralizing epitopes exist among different HPV L1VLPs. The cross-neutralizing activities against nonvaccine types and the enhanced neutralizing activities against earlier immunized vaccine types may result from sequential boosting with these cross-neutralizing epitopes. These observations support early vaccination with more types of L1VLPs derived from HPVs that cause a serious threat to the population.

20.
BMC Gastroenterol ; 19(1): 154, 2019 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-31455284

RESUMO

BACKGROUND: Systemic lupus erythematosus is an autoimmune disease which can affect multiple organs, resulting in significant mortality and morbidity. Lupus enteritis is one of the rare complications of SLE, defined as vasculitis of the intestinal tract, with supportive biopsy findings and/or image. However, lupus enteritis is seldom confirmed on histology or image and the changes of intestinal mucosa are nonspecific. Crohn's disease is a chronic inflammatory disorder of the gastrointestinal tract which affects any part of the gastrointestinal tract. The diagnosis of CD is confirmed by clinical evaluation and a combination of endoscopic, histology, radiology, and/or biochemical investigations. CASE PRESENTATION: Here we report a rare case of a 71-years-old Chinese male has been diagnosed with lupus enteritis which similar to CD in the aspects of endoscopic, histology, and radiology. So far, there are no relevant cases reported. CONCLUSIONS: The endoscopic appearance of lupus enteritis is nonspecific, on the basis of our case, the features of lupus enteritis can be described as spacious, clean and no moss ulcers which discontinuous involved all gastrointestinal tract.


Assuntos
Doença de Crohn/diagnóstico , Endoscopia do Sistema Digestório/métodos , Enterite , Trato Gastrointestinal , Lúpus Eritematoso Sistêmico , Idoso , Anticorpos Antinucleares/sangue , Anticorpos Antifosfolipídeos/sangue , Diagnóstico Diferencial , Enterite/diagnóstico , Enterite/etiologia , Enterite/fisiopatologia , Trato Gastrointestinal/diagnóstico por imagem , Trato Gastrointestinal/patologia , Humanos , Lúpus Eritematoso Sistêmico/sangue , Lúpus Eritematoso Sistêmico/diagnóstico , Lúpus Eritematoso Sistêmico/fisiopatologia , Lúpus Eritematoso Sistêmico/terapia , Masculino , Tomografia Computadorizada por Raios X/métodos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...