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1.
J Clin Sleep Med ; 2020 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-33231167

RESUMO

STUDY OBJECTIVES: Freezing of gait (FOG) severely impairs life quality of Parkinson disease (PD) patients. It remains unclear about the relationship between sleep disturbance and FOG in PD. In this study, we aimed to investigate the relationship between sleep disturbance and FOG in PD. METHODS: First, we assessed clinical characteristics of freezers and nonfreezers in PD patients. Next, we assessed clinical characteristics of PD patients with different PDSS1 score. Finally, we prospectively followed a cohort of nonfreezers at baseline clinical visit for a maximum of 18 months, and performed a Cox regression analysis to further investigate the relationship between PDSS1 score and FOG in PD. RESULTS: A total of 163 participants with PD were included into the baseline analysis. The freezers had significantly worse sleep compared with the nonfreezers. The proportion of freezers in the patients with low PDSS1 score (PDSS1<6) was significantly higher than that in the patients with high PDSS1 score (PDSS1≥6). A total of 52 nonfreezers were prospectively followed. During a maximum of 18-month follow-up, FOG incidence (73%) in the PDSS1<6 group was significantly higher than that (24%) in the PDSS1≥6 group (p =.008). Low PDSS1 score (HR=4.23, 95% CI 1.64-10.92, p =.003) and high L-dopa equivalent daily dose (HR=4.18, 95% CI 1.62-10.75, p =.003) were significantly associated with an increased hazard of FOG. CONCLUSIONS: Our study indicated that low PDSS1 score may be a risk factor for the development of FOG and provided important insights into potential targets for the prevention/delay of FOG in PD.

2.
J Neuroinflammation ; 17(1): 342, 2020 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-33198771

RESUMO

BACKGROUND: Smad3 signaling is indicated to regulate microglia activity. Parkinson's disease (PD) neurodegeneration is shown to be associated with aging and neuroinflammation. However, it remains unclear about the relationship among Smad3 signaling, aging, neuroinflammation, and PD. METHODS: Rats were treated with SIS3 (a specific inhibitor of Smad3, intranigal injection) and/or lipopolysaccharide (intraperitoneal injection). We investigated the effect of SIS3 and lipopolysaccharide and their mechanism of action on motor behavior and nigrostriatal dopaminergic system in the rats. Furthermore, we explored the effect of SIS3 and LPS and their potential signaling mechanism of action on inflammatory response by using primary microglial cultures. Finally, we investigated the relationship among aging, Smad3 signaling, and neuroinflammation using animals of different ages. RESULTS: Both SIS3 and lipopolysaccharide induced significant behavior deficits and nigrostriatal dopaminergic neurodegeneration in the rats compared with the vehicle-treated (control) rats. Significantly increased behavior deficits and nigrostriatal dopaminergic neurodegeneration were observed in the rats co-treated with SIS3 and lipopolysaccharide compared with the rats treated with vehicle, SIS3, or lipopolysaccharide. Furthermore, both SIS3 and lipopolysaccharide induced significant microglia activation and proinflammatory factor (IL-1ß, IL-6, iNOS, and ROS) level increase in the SN of rats compared with the control rats. Significantly enhanced microglial inflammatory response was observed in the rats co-treated with SIS3 and lipopolysaccharide compared with the other three groups. For our in vitro study, both SIS3 and lipopolysaccharide induced significant proinflammatory factor level increase in primary microglia cultures compared with the control cultures. Significantly increased inflammatory response was observed in the cultures co-treated with SIS3 and lipopolysaccharide compared with the other three groups. MAPK (ERK/p38) contributed to microglial inflammatory response induced by co-treatment with SIS3 and lipopolysaccharide. Interestingly, there was decrease in Smad3 and pSmad3 expression (protein) and enhancement of neuroinflammation in the mouse SN with aging. Proinflammatory factor levels were significantly inversely correlated with Smad3 and pSmad3 expression. CONCLUSION: Our study strongly indicates the involvement of SN Smad3 signaling deficiency in aging and PD neurodegeneration and provides a novel molecular mechanism underlying the participation of aging in PD and helps to elucidate the mechanisms for the combined effect of multiple factors in PD.

3.
Huan Jing Ke Xue ; 41(10): 4547-4554, 2020 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-33124386

RESUMO

The emission of nitrogen and phosphorus via non-point source pollution from a small watershed has become the main pollution source of river waters, while climatic conditions and human activities directly affect the changes in rainfall-runoff and types of land use that are closely related to nitrogen and phosphorus pollution. In this study, we explore the runoff loss, nitrogen and phosphors concentration, and nitrogen and phosphorus emission in Huajiaogou small watershed on the upper reaches of Yangtze River. The rainfall, runoff, and temporal changes of nitrogen and phosphorus were analyzed using the continuous position monitoring data. The results showed that:① the runoff volumes were 10.05×105 m3 and 3.34×105 m3 from July 1st to September 30th, accounting for 76.58% and 56.51% in 2012 and 2013, respectively, and they were positively correlated to rainfall. The peak concentrations of ammonia nitrogen (NH4+-N) from April 1st to June 30th were 11.51 mg ·L-1 and 4.44 mg ·L-1in 2012 and 2013, respectively. ② The NH4+-N emission risk period was from July 1st to September 30th, accounting for 78.45% and 62.24% in 2012 and 2013, respectively. The peak concentration and emission risk period of total nitrogen (TN) and nitrate nitrogen (NO3--N) were from July 1st to September 30th, and NO3--N was the main form of the total nitrogen emission. The peak concentration of NO3--N was 6.06 mg ·L-1 and 11.43 mg ·L-1in 2012 and 2013, respectively, and the loss in NO3--N from July 1st to September 30th accounted for 88.74% and 65.55% in 2012 and 2013, respectively. ③The emission risk period of total phosphorus (TP), dissolved total phosphorus (DTP), and particulate phosphorus (PP) was also from July 1st to September 30th, and the particulate phosphorus was the main form of the total phosphorus emission. The particulate phosphorus emission from July 1st to September 30th accounted for 36% and 68% in 2012 and 2013, respectively, and the ration of particle phosphorus to total phosphorus was easily affected by rainfall.


Assuntos
Fósforo , Poluentes Químicos da Água , China , Monitoramento Ambiental , Nitrogênio/análise , Fósforo/análise , Rios , Poluentes Químicos da Água/análise
4.
Acta Odontol Scand ; 78(4): 265-274, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32285744

RESUMO

Objective: Power toothbrushes is considered an effective tool for maintaining oral health; however, its efficacy as compared to manual toothbrushes is still not completely clarified. This article aims to evaluate the efficacy of power toothbrushes compared with the manual toothbrushes in terms of plaque, gingivitis and bleeding reduction.Methods: An electronic search was performed on PUBMED, Web of Science, Wiley and Research Gate. Studies comparing the effectiveness of plaque, gingivitis and bleeding reduction between power and manual toothbrushes were included. Results and effect sizes analysis are presented as standard mean difference (SMD), and subgroup analysis stratified by mode of action of the power toothbrush was performed. Study quality and risk of bias were assessed using the Cochrane assessment tool.Results: A total of 21 randomized clinical studies were included. Power toothbrushes were significantly more effective in reducing plaque index (26 trials: SMD = 0.86, 95% CI: 0.58 to 1.14, I2 = 91.5%, p < .0001), gingival index (14 trials: SMD = 0.47, 95% CI: 0.12 to 0.82, I2 = 88.7%, p < .0001), and bleeding index (11 trials: SMD = 0.92, 95% CI: 0.43 to 1.40, I2 = 91.8%, p < .0001) compared with the manual toothbrushes, except that there was no significant differences between the oscillating-rotating toothbrushes and manual toothbrushes regarding gingivitis reduction (7 trials: SMD = 0.07, 95% CI: -0.20 to 0.33, I2 = 57.2%, p = .03).Conclusions: Power toothbrushes is more effective in reducing dental plaque, gingivitis and bleeding compared with the manual toothbrush.


Assuntos
Placa Dentária/prevenção & controle , Gengivite/prevenção & controle , Saúde Bucal , Escovação Dentária/instrumentação , Dispositivos para o Cuidado Bucal Domiciliar , Índice de Placa Dentária , Desenho de Equipamento , Humanos , Ensaios Clínicos Controlados Aleatórios como Assunto , Método Simples-Cego , Escovação Dentária/métodos
5.
PLoS One ; 15(3): e0230229, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32168331

RESUMO

Poractant alfa and Calsurf are two natural surfactants widely used in China for the treatment of neonatal respiratory distress syndrome, which are extracted from porcine and calf lungs, respectively. The purpose of this experimental study was to compare their in vitro characteristics and in vivo effects in the improvement of pulmonary function and protection of lung injury. The biophysical properties, ultrastructure, and lipid composition of both surfactant preparations were respectively analysed in vitro by means of Langmuir-Blodgett trough (LBT), atomic force microscopy (AFM), and liquid-chromatography mass-spectrometry (LC-MS). Then, as core pharmacological activity, both head-to-head (100 and 200 mg/kg for both surfactants) and licensed dose comparisons (70 mg/kg Calsurf vs. 200 mg/kg Poractant alfa) between the two surfactants were conducted as prophylaxis in preterm rabbits with primary surfactant deficiency, assessing survival time and rate and dynamic compliance of the respiratory system (Cdyn). Intrapulmonary surfactant pools, morphometric volume density as alveolar expansion (Vv), and lung injury scores were determined post mortem. AFM and LC-MS analysis revealed qualitative differences in the ultrastructure as well as in the lipid composition of both preparations. Calsurf showed a longer plateau region of the LBT isotherm and lower film compressibility. In vivo, both surfactant preparations improved Cdyn at any dose, although maximum benefits in terms of Vv and intrapulmonary surfactant pools were seen with the 200 mg/kg dose in both surfactants. The group of animals treated with 200 mg/kg of Poractant alfa showed a prolonged survival time and rate compared to untreated but ventilated controls, and significantly ameliorated lung injury compared to Calsurf at any dose, including 200 mg/kg. The overall outcomes suggest the pulmonary effects to be dose dependent for both preparations. The group of animals treated with 200 mg/kg of Poractant alfa showed a significant reduction of mortality. Compared to Calsurf, Poractant alfa exerted better effects if licensed doses were compared, which requires further investigation.


Assuntos
Produtos Biológicos/farmacologia , Pulmão/efeitos dos fármacos , Fosfolipídeos/farmacologia , Surfactantes Pulmonares/farmacologia , Animais , China , Humanos , Recém-Nascido , Recém-Nascido Prematuro , Coelhos , Respiração Artificial/métodos , Síndrome do Desconforto Respiratório do Recém-Nascido/tratamento farmacológico , Suínos
6.
BMC Plant Biol ; 19(1): 522, 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31775626

RESUMO

BACKGROUND: The WRKYs are a major family of plant transcription factors that play roles in the responses to biotic and abiotic stresses; however, a comprehensive study of the WRKY family in roses (Rosa sp.) has not previously been performed. RESULTS: In the present study, we performed a genome-wide analysis of the WRKY genes in the rose (Rosa chinensis), including their phylogenetic relationships, gene structure, chromosomal locations, and collinearity. Using a phylogenetic analysis, we divided the 56 RcWRKY genes into three subgroups. The RcWRKYs were unevenly distributed across all seven rose chromosomes, and a study of their collinearity suggested that genome duplication may have played a major role in RcWRKY gene duplication. A Ka/Ks analysis indicated that they mainly underwent purifying selection. Botrytis cinerea infection induced the expression of 19 RcWRKYs, most of which had undergone gene duplication during evolution. These RcWRKYs may regulate rose resistance against B. cinerea. Based on our phylogenetic and expression analyses, RcWRKY41 was identified as a candidate regulatory gene in the response to B. cinerea infection, which was confirmed using virus-induced gene silencing. CONCLUSIONS: This study provides useful information to facilitate the further study of the function of the rose WRKY gene family.


Assuntos
Doenças das Plantas/genética , Proteínas de Plantas/genética , Rosa/genética , Fatores de Transcrição/genética , Botrytis , Mapeamento Cromossômico , Cromossomos de Plantas , Resistência à Doença/genética , Duplicação Gênica , Perfilação da Expressão Gênica , Genes de Plantas , Estudo de Associação Genômica Ampla , Filogenia , Doenças das Plantas/microbiologia , Proteínas de Plantas/fisiologia , Rosa/microbiologia , Sintenia , Fatores de Transcrição/fisiologia
7.
World J Gastroenterol ; 25(21): 2650-2664, 2019 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-31210716

RESUMO

BACKGROUND: The available prediction models for clinically relevant postoperative pancreatic fistula (CR-POPF) do not incorporate both preoperative and intraoperative variables. AIM: To construct a new risk scoring system for CR-POPF that includes both preoperative and intraoperative factors. METHODS: This was a retrospective study of patients who underwent pancreaticoduodenectomy (PD) or pylorus-preserving PD (PPPD) between January 2011 and December 2016 at the First Affiliated Hospital of Soochow University. Patients were divided into a study (01/2011 to 12/2014) or validation (01/2015 to 12/2016) group according to the time of admission. POPF severity was classified into three grades: Biochemical leak (grade A) and CR-POPF (grades B and C). Logistic regression was used to create a predictive scoring system. RESULTS: Preoperative serum albumin ≥ 35 g/L [P = 0.032, odds ratio (OR) = 0.92, 95% confidence interval (CI): 0.85-0.99], hard pancreatic texture (P = 0.004, OR = 0.25, 95%CI: 0.10-0.64), pancreatic duct diameter ≥ 3 mm (P = 0.029, OR = 0.50, 95%CI: 0.27-0.93), and intraoperative blood loss ≥ 500 mL (P = 0.006, OR = 1.002, 95%CI: 1.001-1.003) were independently associated with CR-POPF. We established a 10-point risk scoring system to predict CR-POPF. The area under the curve was 0.821 (95%CI: 0.736-0.905) and the cut-off value was 3.5. Including drain amylase levels improved the predictive power of the model. CONCLUSION: This study established a 10-point scoring system to predict CR-POPF after PD/PPPD using preoperative and intraoperative parameters. Ultimately, this system could be used to distinguish between high- and low-risk populations in order to facilitate timely interventions after PD.


Assuntos
Fístula Pancreática/diagnóstico , Pancreaticoduodenectomia/efeitos adversos , Complicações Pós-Operatórias/diagnóstico , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Período Intraoperatório , Masculino , Pessoa de Meia-Idade , Fístula Pancreática/etiologia , Pancreaticoduodenectomia/métodos , Complicações Pós-Operatórias/etiologia , Período Pré-Operatório , Prognóstico , Curva ROC , Estudos Retrospectivos , Medição de Risco/métodos , Índice de Gravidade de Doença , Adulto Jovem
8.
J Matern Fetal Neonatal Med ; : 1-9, 2019 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-31146607

RESUMO

Objectives: We investigated the incidence and outcome of clinical and histological chorioamnionitis (CA) and the associated risk factors in an emerging provincial perinatal center in 2014-2015. Study design: Based on the suspected and proven clinical infection in the third trimester of pregnancy, placenta and accessories from singleton deliveries were routinely examined histologically. The incidence of CA and associated fetal and neonatal outcome were compared by the total deliveries. Results: Of the 14,166 deliveries in 12 months, infection and inflammation were found in 373 out of 2372 (15.7%) placentas subjected to histological examination and diagnosed as CA (2.6%). These cases were divided into four groups as histological CA only (HCA, n = 335), clinical and histological CA (HCC, n = 20), clinical CA only (CCA, n = 18) and non-CA control (CON, n = 1999). Thus, an incidence of histological CA was 2.5% (355/14 166) in this birth population. Compared to CON, HCA group was more likely to have premature rupture of membrane, antenatal antibiotic use, gestational age <34 weeks, fetal distress, positive bacterial culture from vaginal secretions, and early-onset sepsis in newborns (all p ≤ .01), which were associated with perinatal risk factors of CA. In the very preterm subgroups, more stillbirths and death at delivery and neonatal intensive care unit admissions (p < .001) were found in the CA group. Conclusions: The results suggested that histological CA was associated with early-onset sepsis and combined perinatal comorbidities which are of more diagnostic importance than clinical only CA. The placental histological examination should be routinely performed in very preterm births to better assess perinatal and neonatal outcome.

9.
Pediatr Res ; 86(2): 208-215, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31086290

RESUMO

BACKGROUND: The pathogenesis of neonatal group B Streptococcus (GBS) lung infection may be associated with surfactant dysfunction or deficiency. This study aimed to investigate the efficacy of surfactants on early postnatal GBS infection in ventilated newborn rabbit lungs. METHODS: A near-term newborn rabbit model was established by intratracheal GBS instillation immediately at birth, followed by mechanical ventilation. At postnatal 1 h, a porcine surfactant was given intratracheally at 100 or 200 mg/kg. After 6 h, animals were euthanized, and lung and blood samples were collected for bacterial counting. Lung histopathology and messenger RNA (mRNA) expression of inflammatory mediators, surfactant proteins, and growth factors in lung tissue were assessed. RESULTS: The surfactants significantly suppressed (by >50%) pulmonary bacterial proliferation and systemic translocation, alleviated lung inflammatory injury, and improved alveolar expansion by morphometry, in favor of high-dose surfactants. Though the survival rate and lung mechanics were not improved, the surfactants significantly suppressed mRNA expression of proinflammatory mediators, while that for surfactant proteins and growth factors was differentially expressed, compared to the control and GBS infection groups. CONCLUSION: Exogenous surfactants may provide a therapeutic alternative for neonatal lung infection by suppressing pulmonary GBS proliferation and translocation into systemic circulation, alleviating inflammatory injury and regulating growth factor expression.


Assuntos
Pulmão/efeitos dos fármacos , Surfactantes Pulmonares/uso terapêutico , Respiração Artificial , Infecções Estreptocócicas/tratamento farmacológico , Streptococcus agalactiae , Animais , Animais Recém-Nascidos , Líquido da Lavagem Broncoalveolar , Hemólise , Inflamação , Pulmão/fisiopatologia , Lesão Pulmonar/tratamento farmacológico , RNA Mensageiro/metabolismo , Coelhos , Suínos
10.
Nutr Neurosci ; : 1-12, 2019 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-31050314

RESUMO

OBJECTIVES: We aim to investigate the joint effect of iron (enhanced neonatal iron intake), 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and biochanin A (BA, oral administration) and possible mechanisms for action on behavioral and neurochemical indicators in the mice. METHODS: Rotarod test, pole test and swim test were used to evaluate animal behavior. The neurochemical analysis was conducted by HPLC-ECD. Oxidative stress was determined in this study. Further mechanism was investigated through in vitro experiments. RESULTS: Iron and MPTP co-administration significantly induced behavioral deficits and decreased striatal dopamine content in the male and female mice. The co-administration of iron and MPTP also significantly induced redox imbalance in the substantia nigra (SN) of mice. Furthermore, BA significantly improved behavioral deficits and increased striatal dopamine content in the mice co-treated with iron and MPTP. BA also significantly improved redox imbalance in the SN of mice co-administered with iron and MPTP. Finally, we showed that iron and 1-Methyl-4-phenylpyridinium (MPP+) co-treatment significantly increased superoxide production in microglial cultures by inducing p38 mitogen-activated protein kinase (MAPK) activation. BA also significantly decreased superoxide production and p38 MAPK phosphorylation in the cultures co-treated with iron and MPP+. CONCLUSION: Iron and MPTP co-treatment may result in worsened behavioral and neurochemical deficits and aggravated redox imbalance through inducing microglial p38 MAPK activation. BA may improve behavioral and neurochemical deficits and redox imbalance through repressing microglial p38 MAPK activation.

11.
Proteomics Clin Appl ; 13(5): e1800195, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31025496

RESUMO

PURPOSE: Due to a lack of early diagnostic markers, pancreatic cancer (PC) remains a lethal disease. Proteomic approaches are now being applied to identify novel PC biomarkers. EXPERIMENTAL DESIGN: In this study, iTRAQ and LC-MS/MS are used to perform comparative analyses of serum from PC patients and healthy controls (HC), to identify specific serum biomarkers for PC. Serum levels of candidate proteins are determined using ELISA. RESULTS: Among 869 proteins identified, 55 are potential biomarkers; Vitamin K-dependent protein Z (PROZ) and tumor necrosis factor receptor superfamily member 6b (TNFRSF6B) are selected for further analysis. Serum levels of PROZ and TNFRSF6B are significantly higher in PC patients than in HC or pancreatic benign controls (BC) (p < 0.01). The AUCs range from 0.816 to 0.971 for PROZ, TNFRSF6B, and carbohydrate antigen 19-9, either individually or in combination, in PC versus HC+BC, and from 0.711 to 0.932 in PC Stage I versus HC+BC. CONCLUSIONS AND CLINICAL RELEVANCE: It is demonstrated that PROZ and TNFRSF6B are novel serum biomarkers for detecting early stage PC, and for distinguishing PC from pancreatic benign tumor and healthy individuals. Additional large cohort studies are needed to develop PROZ and TNFRSF6B as clinical PC biomarkers.


Assuntos
Biomarcadores Tumorais/metabolismo , Detecção Precoce de Câncer , Neoplasias Pancreáticas/diagnóstico , Neoplasias Pancreáticas/metabolismo , Proteômica , Espectrometria de Massas em Tandem , Adulto , Idoso , Feminino , Ontologia Genética , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem
12.
Materials (Basel) ; 11(9)2018 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-30189616

RESUMO

Plasma electrolytic oxidation (PEO) coatings were produced on AZ31B magnesium alloys in alkaline electrolytes with the addition of various concentrations of Al2O3 sols. Effects of Al2O3 sol concentrations on the microstructure, phase composition, corrosion resistance and hardness of PEO coatings were evaluated by scanning electron microscopy (SEM), X-ray diffraction (XRD), microhardness testing and potentiodynamic polarization measurements, respectively. It was revealed that the Al2O3 sol mostly participated in the formation of the ceramic coating and transferred into the MgAl2O4 phase. With the increase of the Al2O3 sol concentration in the range of 0⁻6 vol%, the coating performance in terms of the microstructure, diffraction peak intensity of the MgAl2O4 phase, corrosion resistance and microhardness was improved. Further increase of Al2O3 sol addition did not generate better results. This indicated that 6 vol% might be the proper Al2O3 sol concentration for the formation of PEO coatings.

13.
Int J Med Microbiol ; 307(6): 321-328, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28610835

RESUMO

Biofilms are difficult to eradicate due to their resistance to antibiotics and host immune cells. Ultrasound microbubbles have emerged as a new treatment modality with the underlying mechanisms largely unknown. In this study, we exposed 24-h-old Staphylococcus epidermidis biofilms established in OptiCell™ chambers to ultrasound in combination with microbubbles, and investigated the activities of vancomycin and neutrophils against S. epidermidis biofilms after treatment. The antibiofilm mechanims of ultrasound microbubbles were explored in terms of bacterial permeability and biofilm-associated gene expression. After treatment of ultrasound (1MHz, 0.5W/cm2, 50% duty cycle) combined with microbubbles in the concentration of 1% and 4% (v/v) for 5min, bacterial permeability to extracellular fluorescent dyes was enhanced and the expression of icaA was down-regulated while that of agrB and RNAIII up-regulated. Post-treatment biofilms were more sensitive to vancomycin by demonstrating reduced biomass than those exposed to vancomycin alone (P<0.05). The phagocytosis, oxidative burst activity as well as chemotaxis of neutrophils in response to biofilms were also significantly increased. The bioeffect of ultrasound combined with microbubbles was generally more significant than that of ultrasound alone, and dependent on microbubble concentration. This study demonstrated that ultrasound combined with microbubbles could enhance the activities of antibiotics and neutrophils against biofilms possibly via mechanical and biochemical mechanisms, and may provide an efficient and non-invasive antibiofilm alternative apart from chemical and biological approaches.


Assuntos
Antibacterianos/farmacologia , Biofilmes/efeitos dos fármacos , Microbolhas/uso terapêutico , Sonicação/métodos , Infecções Estafilocócicas/terapia , Staphylococcus epidermidis/efeitos dos fármacos , Animais , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Permeabilidade da Membrana Celular/efeitos dos fármacos , Quimiotaxia/efeitos dos fármacos , Farmacorresistência Bacteriana/efeitos dos fármacos , Corantes Fluorescentes/farmacologia , Humanos , Neutrófilos , Fagocitose/efeitos dos fármacos , RNA Bacteriano/genética , RNA Bacteriano/metabolismo , Coelhos , Explosão Respiratória/efeitos dos fármacos , Vancomicina/farmacologia
14.
Reprod Sci ; 24(5): 783-789, 2017 05.
Artigo em Inglês | MEDLINE | ID: mdl-27678097

RESUMO

The aim of the present study was to determine a predictive model for early-onset preeclampsia with fetal growth restriction (FGR) to be used at 11+0 to 13+6 gestational weeks, by combining the maternal serum level of pregnancy-associated plasma protein-A (PAPP-A), placental growth factor (PLGF), placental protein 13 (PP13), soluble endoglin (sEng), mean arterial pressure (MAP), and uterine artery Doppler. This was a retrospective cohort study of 4453 pregnant women. Uterine artery Doppler examination was conducted in the first trimester. Maternal serum PAPP-A, PLGF, PP13, and sEng were measured. Mean arterial pressure was obtained. Women were classified as with/without early-onset preeclampsia, and women with preeclampsia were classified as with/without FGR. Receiver operating characteristic analysis was performed to determine the value of the model. There were 30 and 32 pregnant women with early-onset preeclampsia with and without FGR. The diagnosis rate of early-onset preeclampsia with FGR was 67.4% using the predictive model when the false positive rate was set at 5% and 73.2% when the false positive rate was 10%. The predictive model (MAP, uterine artery Doppler measurements, and serum biomarkers) had some predictive value for the early diagnosis (11+0 to 13+6 gestational weeks) of early-onset preeclampsia with FGR.


Assuntos
Retardo do Crescimento Fetal/diagnóstico , Pré-Eclâmpsia/diagnóstico , Adulto , Pressão Arterial , Biomarcadores/metabolismo , Endoglina/sangue , Feminino , Retardo do Crescimento Fetal/sangue , Retardo do Crescimento Fetal/fisiopatologia , Galectinas/sangue , Idade Gestacional , Humanos , Pré-Eclâmpsia/sangue , Pré-Eclâmpsia/fisiopatologia , Gravidez , Proteínas da Gravidez/sangue , Proteína Plasmática A Associada à Gravidez/metabolismo , Curva ROC , Estudos Retrospectivos , Ultrassonografia Doppler , Artéria Uterina/diagnóstico por imagem
15.
Pancreas ; 42(8): 1255-61, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-24152952

RESUMO

OBJECTIVES: Early, efficient, and accurate evaluation for organ failure is an important step for improving outcome in severe acute pancreatitis (SAP). We aim to develop a method that can early, efficiently, and accurately evaluate the in-hospital organ failure in patients with SAP. METHODS: Using multivariate logistic regression analysis, the associative factors for in-hospital organ failure were evaluated retrospectively from conventional data obtained from 393 patients with SAP from 2000 to 2012. In classification and regression tree analysis, a new clinical scoring system was developed for the evaluation of in-hospital organ failure in SAP. We also compared the accuracy of our new scoring system with multiple organ dysfunction score and Acute Physiology and Chronic Health Examination II score by the receiver operating characteristic curve. RESULTS: Laboratory results revealed serum calcium level greater than or equal to 1.84 mmol/L, serum creatinine level greater than or equal to 110 µmol/L, age greater than or equal to 72 years, activated partial thromboplastin time less than or equal to 30.95 seconds, and Balthazar computed tomography score greater than or equal to 7 (CCAAB) score system, each contributed 1 point for the prediction of organ failure. The area under the curve of the CCAAB score system was similar to multiple organ dysfunction scores and Acute Physiology and Chronic Health Examination II scores. CONCLUSIONS: The new scoring system CCAAB is an efficient and accurate method for the early evaluation of patients with SAP for in-hospital organ failure.


Assuntos
Medicina Baseada em Evidências/métodos , Pacientes Internados/estatística & dados numéricos , Insuficiência de Múltiplos Órgãos/diagnóstico , Pancreatite/complicações , Doença Aguda , Adulto , Idoso , Idoso de 80 Anos ou mais , Cálcio/sangue , Creatina/sangue , Diagnóstico Precoce , Feminino , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Insuficiência de Múltiplos Órgãos/sangue , Insuficiência de Múltiplos Órgãos/complicações , Análise Multivariada , Pancreatite/patologia , Prognóstico , Curva ROC , Estudos Retrospectivos , Índice de Gravidade de Doença , Tomografia Computadorizada por Raios X , Adulto Jovem
16.
Ultrasonics ; 49(2): 276-80, 2009 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19010508

RESUMO

The influence of the pore shapes on the band structures in phononic crystals with periodic distributed void pores are investigated in this paper. By using finite difference time domain (FDTD) scheme, the dispersion properties of the in-plane x-y mode waves in the materials with triangular, circular or square pores are discussed respectively. The influence of the pore shapes and the porosity on the band gap structures is analyzed. The results show that for x-y mode waves, the stop bands are easily formed in the materials with triangular pores, but hard for square ones. Moreover, a critical porosity exists for the formation of the absolute band gaps. Along with the increase of the porosity, the width of the absolute band gap is increased, but the centre frequency is dropped.

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