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1.
Front Psychol ; 13: 739898, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35369246

RESUMO

The COVID-19 pandemic cropping up at the end of 2019 started to pose a threat to millions of people's health and life after a few weeks. Nevertheless, the COVID-19 pandemic gave rise to social and economic problems that have changed the progress steps of individuals and the whole nation. In this study, the work conditions for employees from Taiwan, Malaysia, and the Chinese mainland are explored and compared, and the relationship between support mechanisms and innovation behaviors (IB) is evaluated with a view of the social cognitive career theory. This study adopts the cross-sectional survey and purposive sampling to collect questionnaires. A total of 623 copies of a questionnaire from Taiwanese, 440 copies from Malaysians, and 513 copies from mainlanders were collected in this study to compare the three groups in developing employees' IBs. Smart-partial least squares for partial least squares structural equation modeling was applied in the structural model to conduct a verification of the hypotheses and comparative analysis in this study. According to the findings, compared with employees from the Chinese mainland, the Taiwanese and Malaysian samples show more significant paths regarding employee employability, IB, prior knowledge, perceived organizational support, self-efficacy, and job performance. Our results will offer more insights and advice concerning theories of human resource.

2.
Front Oncol ; 12: 930611, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36072793

RESUMO

Background: Biliary tract cancer (BTC) is an uncommon but highly lethal malignancy with poor clinical outcomes. To promote the development of precision medicine for BTC, uncovering its genomic profile becomes particularly important. However, studies on the genomic feature of Chinese BTC patients remain insufficient. Methods: A total of 382 Chinese patients with BTC were enrolled in this study, including 71 with intrahepatic cholangiocarcinoma (ICC), 194 with extrahepatic cholangiocarcinoma (ECC), and 117 with gallbladder carcinoma (GBC). Genetic testing was performed by utilizing the next-generation sequencing (NGS) of 499 cancer-related genes and the results were compared to those of Western BTC patients (MSKCC cohorts). Results: The most prevalent genes were TP53 (51.6%), ARID1A (25.9%), KMT2C (24.6%), NCOR1 (17%), SMAD4 (15.2%), KRAS (14.9%), KMT2D (14.9%), ATM (14.1%), and APC (13.9%) in Chinese BTC patients. TP53, SMAD4, and APC were more prevalent in GBC, ECC, and ICC, respectively. In addition, 10.5% of Chinese BTC patients harbored pathogenic or likely pathogenic (P/LP) germline alterations in 41 genes, which were mainly related to DNA damage repair (DDR). Additionally, the genomic features of Chinese and Western BTC tumors were similar, with the exception of the notable difference in the prevalence of TP53, KRAS, IDH1, KMT2C, and SMAD4. Notably, Chinese BTC patients had high prevalence (57.1%) of actionable alterations, especially for those with ECC, and half (192/382) of them had somatic DDR alterations, with the prevalence of deleterious ones being significantly higher than their Western counterparts. Twenty-three percent of patients had a higher tumor mutational burden (TMB-H, over 10 mutations/MB), and TMB was significantly higher in those with deleterious DDR alterations and/or microsatellite instability-high. The most common mutational signature in BTC patients was Signature 1, and interestingly, Signatures 1, 4, and 26 were significantly associated with higher TMB level, but not with the survival of patients who had received immunotherapy in pan-cancer. Conclusion: Our study elaborated the distinct germline and somatic genomic characteristics of Chinese BTC patients and identified clinically actionable alterations, highlighting the possibility for the development and application of precision medicine.

3.
Front Public Health ; 10: 815579, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36062135

RESUMO

Aims: To reveal the impact of eleven risk factors on stroke and provide estimates of the prevention potential. Methods: We completed a multicenter case-control study in Jiangxi, China, a middle-income area. Neuroimaging examination was performed in all cases. Controls were stroke-free adults recruited from the community in the case concentration area. Conditional logistic regression and unconditional logistic regression were used for subgroup analysis of stroke type, and other groups (sex, age and urban-rural area), respectively. Odds ratios (ORs) and their population attributable risks (PARs) were calculated, with 95% confidence intervals. Results: A total of 43,615 participants (11,735 cases and 31,880 controls) were recruited from February to September 2018, of whom we enrolled 11,729 case-control pairs. Physical inactivity [PAR 69.5% (66.9-71.9%)] and hypertension [53.4% (49.8-56.8%)] were two major risk factors for stroke, followed by high salt intake [23.9% (20.5-27.3%)], dyslipidemia [20.5% (17.1-24.0%)], meat-based diet [17.5% (14.9-20.4%)], diabetes [7.7% (5.9-9.7%)], cardiac causes [5.3% (4.0-6.7%)], alcohol intake [4.7% (0.2-10.0%)], and high homocysteine [4.3% (1.4-7.4%)]. Nine of these factors were associated with ischemic stroke, and five were associated with intracerebral hemorrhage. Collectively, eleven risk factors accounted for 59.9% of the PAR for all stroke (ischemic stroke: 61.0%; intracerebral hemorrhage: 46.5%), and were consistent across sex (men: 65.5%; women: 62.3%), age (≤55: 65.2%; >55: 63.5%), and urban-rural areas (city: 62.2%; county: 65.7%). Conclusion: The 11 risk factors associated with stroke identified will provide an important reference for evidence-based planning for stroke prevention in middle-income areas. There is an urgent need to improve awareness, management and control of behavioral and metabolic risk factors, particularly to promote physical activity and reduce blood pressure.


Assuntos
AVC Isquêmico , Acidente Vascular Cerebral , Adulto , Estudos de Casos e Controles , Hemorragia Cerebral/complicações , Hemorragia Cerebral/epidemiologia , China/epidemiologia , Feminino , Humanos , Masculino , Fatores de Risco , Acidente Vascular Cerebral/epidemiologia , Acidente Vascular Cerebral/etiologia , Acidente Vascular Cerebral/prevenção & controle
4.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 44(4): 570-578, 2022 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-36065688

RESUMO

Objective To systematically review the overall status of postoperative recurrence in patients with atypical meningiomas. Methods China National Knowledge Infrastructure,Wanfang Database,Chinese Biomedical Literature Database,VIP Database,PubMed,Embase,Web of Science,and Cochrane Library were searched for collection of the relevant literature on the recurrence of atypical meningioma from database establishment to July 2021.Two investigators independently screened the literature,extracted data,and assessed the risk of bias of the included studies,and then performed a meta-analysis by using R 5.0. Results A total of 29 studies involving 3122 patients were included in this study.The meta-analysis showed that the overall postoperative recurrence rate of atypical meningioma was 38%.The subgroup analysis showed that the tumor recurrence rate of patients ≥60 years old and<60 years old was 51% and 40%,respectively,with no significant difference.The tumor recurrence rates in male and female patients were 42% and 44%,respectively,which showed no significant difference.The recurrence rates of the patients with parasagittal meningiomas,brain tissue infiltration,Ki-67>8%,mitotic count ≥6/10 high-power fields,and tissue necrosis were 52%,47%,63%,53%,and 69%,respectively.The recurrence rate after subtotal tumor resection was as high as 58%,and the patients who received radiotherapy had higher tumor recurrence rate than that those who did not receive radiotherapy (38% vs.29%,P=0.007). Conclusions The current evidence demonstrates that atypical meningioma has a high recurrence rate after surgery.It is essential to pay more attention and take corresponding measures to improve the tumor-free survival rate of the patients.


Assuntos
Neoplasias Meníngeas , Meningioma , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia , Período Pós-Operatório , Fatores de Risco
5.
Mol Plant Pathol ; 2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36071584

RESUMO

Grapevine downy mildew is one of the most devastating diseases in grape production worldwide, but its pathogenesis remains largely unknown. A thorough understanding of the interaction between grapevine and the causal agent, Plasmopara viticola, is helpful to develop alternative disease control measures. Effector proteins that could be secreted to the interaction interface by pathogens are responsible for the susceptibility of host plants. In this study, a Crinkler effector, named PvCRN17, which is from P. viticola and showed virulent effects towards Nicotiana benthamiana previously, was further investigated. Consistently, PvCRN17 showed a virulent effect on grapevine plants. Protein-protein interaction experiments identified grapevine VAE7L1 (Vitis protein ASYMMETRIC LEAVES 1/2 ENHANCER 7-Like 1) as one target of PvCRN17. VAE7L1 was found to interact with VvCIA1 and VvAE7, thus it may function in the cytosolic iron-sulphur cluster assembly (CIA) pathway. Transient expression of VAE7L1 in Vitis riparia and N. benthamiana leaves enhanced the host resistance to oomycete pathogens. Downstream of the CIA pathway in grapevine, three iron-sulphur (Fe-S) proteins showed an enhancing effect on the disease resistance of N. benthamiana. Competitive co-immunoprecipitation assay showed PvCRN17 could compete with VvCIA1 to bind with VAE7L1 and VvAE7. Moreover, PvCRN17 and VAE7L1 were colocalized at the plasma membrane of the plant cell. To conclude, after intruding into the grapevine cell, PvCRN17 would compete with VCIA1 to bind with VAE7L1 and VAE7, demolishing the CIA Fe-S cluster transfer complex, interrupting the maturation of Fe-S proteins, to suppress Fe-S proteins-mediated defence responses.

6.
Orthop J Sports Med ; 10(8): 23259671221119225, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-36051975

RESUMO

Background: Currently, there is no consensus regarding the accuracy of magnetic resonance imaging (MRI) in the detection of acetabular chondral delamination (ACD) in patients with femoroacetabular impingement (FAI), and, correspondingly, the preoperative diagnosis of ACD remains challenging. Hypothesis: It was hypothesized that MRI would have relatively high accuracy in detecting ACD in patients with FAI. Study Design: Cohort study (diagnosis); Level of evidence, 2. Methods: We retrospectively evaluated patients who attended the sports medicine clinic of our department and underwent arthroscopic surgery for the diagnosis of FAI between January 2018 and December 2020. All patients underwent preoperative 3.0-T MRI. ACD was evaluated by 2 raters on 3.0-T MRI scans, and interrater and intrarater reliability was assessed. Sensitivity, specificity, accuracy, positive predictive value (PPV), and negative predictive value (NPV) of MRI for diagnosis of ACD were calculated, using arthroscopic surgery as the standard. Results: A total of 233 patients (mean age, 37.4 years; 99 male and 134 female) were included in this study. The presence of ACD in 101 (43.3%) patients was confirmed during hip arthroscopy. The intraobserver reliability of both of the observers in detecting ACD using 3.0-T MRI scans was almost perfect (observer 1, kappa coefficient [κ] = 0.909 [95% CI, 0.854-0.964]; observer 2, κ = 0.937 [95% CI, 0.890-0.984]), and the interobserver reliability between the observers (κ = 0.801 [95% CI, 0.723-0.879]) was substantial. The overall sensitivity, specificity, PPV, and NPV of preoperative MRI to detect ACD were 83.7%, 82%, 74.2%, and 89.1%, respectively. Conclusion: It was found that 3.0-T MRI had a relatively high sensitivity, specificity, PPV, and NPV for diagnosis of ACD in patients with FAI and could be a reliable method of diagnosing ACD preoperatively.

7.
mSystems ; : e0053422, 2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36073803

RESUMO

Caproate, an important medium-chain fatty acid, can only be synthesized by limited bacterial species by using ethanol, lactate, or certain saccharides. Caproicibacterium lactatifermentans is a promising caproate producer due to its glucose and lactate utilization capabilities. However, the global cellular responses of this bacterium to different carbon sources were not well understood. Here, C. lactatifermentans showed robust growth on glucose but more active caproate synthesis on lactate. Comparative transcriptome revealed that the genes involved in reverse ß-oxidation for caproate synthesis and V-type ATPase-dependent ATP generation were upregulated under lactate condition, while several genes responsible for biomass synthesis were upregulated under glucose condition. Based on metabolic pathway reconstructions and bioenergetics analysis, the biomass accumulation on glucose condition may be supported by sufficient supplies of ATP and metabolite intermediates via glycolysis. In contrast, the ATP yield per glucose equivalent from lactate conversion into caproate was only 20% of that from glucose. Thus, the upregulation of the reverse ß-oxidation genes may be essential for cell survival under lactate conditions. Furthermore, the remarkably decreased lactate utilization was observed after glucose acclimatization, indicating the negative modulation of lactate utilization by glucose metabolism. Based on the cotranscription of the lactate utilization repressor gene lldR with sugar-specific PTS genes and the opposite expression patterns of lldR and lactate utilization genes, a novel regulatory mechanism of glucose-repressed lactate utilization mediated via lldR was proposed. The results of this study suggested the molecular mechanism underlying differential physiologic and metabolic characteristics of C. lactatifermentans grown on glucose and lactate. IMPORTANCE Caproicibacterium lactatifermentans is a unique and robust caproate-producing bacterium in the family Oscillospiraceae due to its lactate utilization capability, whereas its close relatives such as Caproicibacterium amylolyticum, Caproiciproducens galactitolivorans, and Caproicibacter fermentans cannot utilize lactate but produce lactate as the main fermentation end product. Moreover, C. lactatifermentans can also utilize several saccharides such as glucose and maltose. Although the metabolic versatility of the bacterium makes it to be a promising industrial caproate producer, the cellular responses of C. lactatifermentans to different carbon sources were unknown. Here, the molecular mechanisms of biomass synthesis supported by glucose utilization and the cell survival supported by lactate utilization were revealed. A novel insight into the regulatory machinery in which glucose negatively regulates lactate utilization was proposed. This study provides a valuable basis to control and optimize caproate production, which will contribute to achieving a circular economy and environmental sustainability.

8.
Nanoscale ; 2022 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-36065785

RESUMO

Diabetic wound infection is a common disease that has significantly reduced people's quality of life. Although tremendous achievements have been made in clinical treatment, the crucial challenge in diabetic infected wound management stems from the detrimental diabetic wound environment and the emergence of bacterial resistance after long-term medication, which result in a reduced efficacy, an increased dosage of medication, and severe side effects. To tackle these issues, it is of great significance to develop an innovative treatment strategy for diabetic wound infection therapy. Currently, the exploitation of nanobiomaterial-based therapeutic systems for diabetic infected wounds is booming, and therapeutic systems with a stimuli-responsive performance have received extensive attention. These therapeutic systems are able to accelerate diabetic infected wound healing due to the on-demand release of therapeutic agents in diabetic infected wounds in response to stimulating factors. Based on the characteristics of diabetic infected wounds, many endogenous stimuli-responsive (e.g., glucose, enzyme, hypoxia, and acidity) therapeutic systems have been employed for the targeted treatment of infected wounds in diabetic patients. Additionally, exogenous stimulants, including light, magnetism, and temperature, are also capable of achieving on-demand drug release and activation. In this review, the characteristics of diabetic infected wounds are presented, and then exogenous/endogenous stimuli therapeutic systems for the treatment of diabetic infected wounds are summarized. Finally, the current challenges and future outlook of stimuli-responsive therapeutic systems are also discussed.

9.
Artigo em Inglês | MEDLINE | ID: mdl-36066917

RESUMO

Cyclopolymerization is a powerful method for synthesizing polyacetylenes containing four- to seven-membered rings. However, the structure of the repeat unit only consists of mono-cycloalkene due to the single cyclization of diyne monomers. Herein, we demonstrate a novel cascade cyclopolymerization to synthesize polyacetylenes containing fused bicyclic rings from triyne monomers containing bulky dendrons via sequential cascade ring-closing metathesis. These dendrons provided solubility and stability to the rigid bicyclic polyacetylene backbone. In addition, we controlled the regioselectivity of the catalyst approach by altering its structure and synthesized polymers containing fused bicyclo[4,3,0] or [4,4,0] rings with high molecular weights of up to 120 kg/mol. Interestingly, the resulting polymers showed narrower band gaps (down to 1.6 eV) than polymers with mono-cycloalkene repeat units due to the planarization of the conjugated segment resulting from the fused bicyclic structure.

10.
Sci Rep ; 12(1): 14958, 2022 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-36056127

RESUMO

Urban road DEM is not only an important basic geographic information data of the city, but also an important element to describe and express the urban topography, and it is an indispensable part of the construction of the smart digital city, urban planning and urban surface process simulation. Previous methods for constructing urban road DEMs do not sufficiently consider the actual morphological characteristics of urban roads, and morphological distortion is evident in the expression of urban roads, seriously affecting the application of urban rainfall flood simulation and urban pipe network design. In response to these problems, this study proposed a considering morphological characteristics fine (CMCF) method of urban road DEM construction, selected a typical urban road area in the Jianye District of Nanjing City in China as the study area, used the 1:500 digital line graphic as data source, hierarchized roads in accordance with different morphological characteristics from the perspective of DEM construction, constructed the corresponding DEMs, and finally merged all levels of road DEMs to produce a complete high-precision urban road DEM. Results showed that the DEM constructed using the CMCF method not only exhibited higher elevation accuracy than the urban road DEM constructed using previous methods, i.e., inverse distance weighting (IDW) and triangulated irregular network (TIN) methods, with a mean error and a root-mean-square error of 0.015 and 0.060, respectively, but it can also accurately express the spatial element composition form and road morphological characteristics of urban roads, avoiding the distorted expression of road morphological characteristics. This study can provide a reference for a new DEM construction method and data support for smart digital city construction and urban surface simulation.

11.
J Hazard Mater ; 439: 129671, 2022 10 05.
Artigo em Inglês | MEDLINE | ID: mdl-36104900

RESUMO

The extracellular electron transfer (EET) is regarded as one of the crucial factors that limit the application of the bioelectrochemical system (BES). In this study, two different solid-phase redox mediators (RMs), biochar (1.2 g/L, T-B) and humin (1.2 g/L, T-H) were used for boosting the microorganisms accessing the electrons required for 2,4,6-TCP dechlorination under weak electrical stimulation (-0.278 V vs. Standard hydrogen electrode). BES with dissolved RM anthraquinone-2,6-disulfonate (AQDS 0.5 mmol/L, T-A) was used as a comparison. The results showed that dechlorination of 2,4,6-TCP could be greatly accelerated by biochar (1.78 d-1) and humin (1.50 d-1) than AQDS (0.24 d-1) and no RM control (T-M, 0.27 d-1). Moreover, phenol became the predominant dechlorination product in T-H (78.5 %) and T-B (63.0 %) instead of 4-CP in T-M (67.1 %) and T-A (89.8 %). Pseudomonas, Sulfurospirillum, Desulfuromonas, Dehalobacter, Anaeromyxobacter, and Dechloromonas belonging to Proteobacteria or Firmicutes rather than Chloroflexi might be responsible for the dechlorination activity. Notably, different RMs tended to stimulate distinct electroactive bacteria. Pseudomonas was the most abundant microorganism in T-M (41.92 %) and T-A (17.24 %), while Rhodobacter was most prevalent in T-H (20.04 %) and Azonexus was predominant in T-B (48.48 %). This study is essential in advancing the understanding of EET in BES for microbial degradation of organohalide contaminants under weak electrical stimulation.


Assuntos
Halogenação , Ácidos Alcanossulfônicos , Biodegradação Ambiental , Carvão Vegetal , Clorofenóis , Estimulação Elétrica , Substâncias Húmicas
12.
Huan Jing Ke Xue ; 43(9): 4475-4483, 2022 Sep 08.
Artigo em Chinês | MEDLINE | ID: mdl-36096588

RESUMO

Primary biological aerosol particles (PBAP) are an important part of ambient aerosols. Both living and dead organisms not only influence human health and air quality but also play important roles in regulating certain atmospheric processes and affect the hydrological cycle and climate change. In this study, flow cytometry (FCM) was utilized in combination with the simultaneous use of permeant (SYBR Green I) and impermeant (propidium iodide, PI) nucleic acid fluorescent staining to detect and quantify the viable and dead airborne biological particles. At the same time, based on this method, the dead/viable PBAP in a Beijing urban area was detected and quantified. Moreover, the influence of environmental factors on the concentrations of primary biological aerosol particles was illuminated. The results showed that the media number concentration of dead and alive PBAP in the Beijing urban area during summer (1.03×106 m-3 and 7.43×105 m-3, respectively) were higher than those during winter (7.34×105 m-3and 6.18×105 m-3, respectively). Statistical analysis showed that there was no significant correlation between PBAP number concentration and environmental factors, i.e., meteorological conditions and air quality, showing a weak positive correlation with temperature and humidity and weak negative correlations with O3, maximum wind speed, and sunshine duration. The number concentration of PBAP was weakly correlated with the mass concentration of PM2.5 but positively correlated with that of coarse particulate matter (PM2.5-10). Both stable weather and dust transport could increase the number concentration of PBAP in Beijing.


Assuntos
Poluentes Atmosféricos , Aerossóis/análise , Poluentes Atmosféricos/análise , Pequim , Monitoramento Ambiental , Humanos , Material Particulado/análise
13.
Pharmacol Res ; 184: 106422, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-36058431

RESUMO

Targeting cardiomyocyte plasticity has emerged as a new strategy for promoting heart repair after myocardial infarction. However, the precise mechanistic network underlying heart regeneration is not completely understood. As noncoding RNAs, circular RNAs (circRNAs) play essential roles in regulating cardiac physiology and pathology. The present study aimed to investigate the potential roles of circMdc1 in cardiac repair after injury and elucidate its underlying mechanisms. Here, we identified that circMdc1 levels were upregulated in postnatal mouse hearts but downregulated in the regenerative myocardium. The expression of circMdc1 in cardiomyocytes is sensitive to oxidative stress, which was attenuated by N-acetyl-cysteine. Enforced circMdc1 expression inhibited cardiomyocyte proliferation, while circMdc1 silencing led to cardiomyocyte cell cycle re-entry. In vivo, the cardiac-specific adeno-associated virus-mediated knockdown of circMdc1 promoted cardiac regeneration and heart repair accompanied by improved heart function. Conversely, circMdc1 overexpression blunted the regenerative capacity of neonatal hearts after apex resection. Moreover, circMdc1 was able to block the translation of its host gene Mdc1 specifically by binding to PABP, affecting DNA damage and the chromosome stability of cardiomyocytes. Furthermore, overexpression of Mdc1 caused damaged mouse hearts to regenerate and repair after myocardial infarction in vivo. Oxidative stress-sensitive circMdc1 plays an important role in cardiac regeneration and heart repair after injury by regulating DNA damage and chromosome stability in cardiomyocytes by blocking the translation of the host gene Mdc1.

15.
Zhongguo Dang Dai Er Ke Za Zhi ; 24(9): 988-993, 2022.
Artigo em Chinês | MEDLINE | ID: mdl-36111716

RESUMO

OBJECTIVES: To investigate the current status of antibiotic use in very low birth weight/extremely low birth weight infants in Jiangsu Province of China, and to provide a clinical basis for the quality and improvement of antibiotic management in the neonatal intensive care unit (NICU). METHODS: A retrospective analysis was performed on the data on general conditions and antibiotic use in the very low birth weight/extremely low birth weight infants who were admitted to 15 hospitals of Jiangsu Province from January 1, 2019 to December 31, 2020. A questionnaire containing 10 measures to reduce antibiotic use was designed to investigate the implementation of these intervention measures. RESULTS: A total of 1 920 very low birth weight/extremely low birth weight infants were enrolled, among whom 1 846 (96.15%) were treated with antibiotic, and the median antibiotic use rate (AUR) was 50/100 patient-days. The AUR ranged from 24/100 to 100/100 patient-days in the 15 hospitals. After adjustment for the confounding factors including gestational age, birth weight, and neonatal critical score, the Poisson regression analysis showed that there was a significant difference in the adjusted AUR (aAUR) among the hospitals (P<0.01). The investigation results showed that among the 10 measures to reduce antibiotic use, 8 measures were implemented in less than 50% of these hospitals, and the number of intervention measures implemented was negatively correlated with aAUR (rs=-0.564, P=0.029). CONCLUSIONS: There is a high AUR among the very low birth weight/extremely low birth weight infants in the 15 hospitals of Jiangsu Province, with a significant difference among hospitals. The hospitals implementing a relatively few measures to reduce antibiotic use tend to have a high AUR. It is expected to reduce AUR in very low birth weight/extremely low birth weight infants by promoting the quality improvement of antibiotic use management in the NICU.


Assuntos
Antibacterianos , Recém-Nascido de Peso Extremamente Baixo ao Nascer , Antibacterianos/uso terapêutico , China , Hospitais , Humanos , Lactente , Recém-Nascido , Estudos Retrospectivos , Inquéritos e Questionários
16.
Artigo em Inglês | MEDLINE | ID: mdl-36123446

RESUMO

To investigate the effects of different types of land use and soil depths on the distributions of heavy metals in the soil in mining areas, heavy metals in different soil layers of five types of agricultural land in the Tongguan gold mining area were studied. The results revealed that the land use type had a greater impact than soil layers on the distribution of heavy metals in the soil. Among the five types of agricultural land examined, the risk values were only exceeded for the heavy metals lead(Pb) and mercury(Hg) in the pepper field, indicating combined pollution of Pb and Hg. Furthermore, some of the heavy metals, such as Pb, zinc(Zn), cadmium(Cd) and Hg, were highly significantly and positively correlated with each other. The pepper field should be monitored to prevent pollution from other heavy metals.

17.
J Org Chem ; 87(18): 12447-12454, 2022 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-36048432

RESUMO

A simple and efficient method for the preparation of selenyl-substituted quinoline derivatives through a CSp3-H selenylation of in situ-generated 3-acetyl quinoline has been developed. This protocol is easy to handle, scalable, and good functional group tolerant, providing a rapid method to 3-selenoacetyl quinoline and 3-diselenoacetyl quinoline derivatives.


Assuntos
Antiprotozoários , Quinolinas
18.
Cancer Cytopathol ; 2022 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-36103360

RESUMO

BACKGROUND: Sputum cell-free DNA (cfDNA) has been confirmed to be a valued surrogate sample for detection of EGFR mutations in patients with lung adenocarcinoma (LAC). Whether it is suitable for detection of mutations of multiple driver genes has not been reported. METHODS: A total of 83 patients with LAC were enrolled and their sputum and paired tumor samples were collected. A next-generation sequencing (NGS)-based 10-gene panel was used to test sputum supernatant-derived cfDNA and paired tumor DNA. The sputum sediments were used for cytological evaluation. RESULTS: The total positive rates of hotspot mutations of the 10 driver genes in sputum cfDNA and matched tissue samples were 65.1% and 77.1%, respectively. The overall detection sensitivity of variants in sputum cfDNA was 81.3% (95% confidence interval [CI], 69.2%, 89.5%) and the specificity was 100% (95% CI, 79.1%, 100%). The sensitivities of testing sputum cfDNA from patients with stage IIIB-IV was 87.0% (95% CI, 74.5%, 94.1%); the sensitivities of testing sputum cfDNA from patients with malignant sputum was 92.3% (95% CI, 78.0%, 98.0%); and the sensitivity of testing sputum cfDNA from patients with malignant sputum in stage IIIB-IV were 94.1% (95% CI, 78.9%, 99.0%). CONCLUSIONS: This study demonstrated that sputum cfDNA were successfully used for the detection of multiple driver genes by NGS. Sputum cfDNA could be a valuable surrogate clinical sample for all-in-one test of mutations to guide target therapies, especially for patients with advanced LAC and malignant sputum.

19.
Appl Microbiol Biotechnol ; 106(18): 6125-6137, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36056198

RESUMO

The combination of Escherichia coli BL21 (DE3) and the pET expression system is used extensively for the expression of various recombinant proteins (RPs). However, RP overexpression often introduces a growth burden for the host, especially in the case of toxic proteins. The key to solving this problem is to reduce the host burden associated with protein overproduction, which is often achieved by regulating the expression or activity of T7 RNAP or growth-decoupled systems. However, these strategies mainly relieve or interrupt the robbing of host resources, and do not eliminate other types of host burdens in the production process. In this study, we constructed a production system based on a dynamic equilibrium to precisely relieve the host burden and increase the RP production. The system is composed of three modules, including the overexpression of basic growth-related genes (rRNA, RNAP core enzyme, sigma factors), prediction and overexpression of key proteins using the enzyme-constrained model ec_iECBD_1354, and dynamic regulation of growth-related and key protein expression intensity based on a burden-driven promoter. Using this system, the production of many high-burden proteins, including autolysis protein and E. coli membrane proteins, was increased to varying degrees. Among them, the cytosine transporter protein (CodB) was most significantly improved, with a 4.02-fold higher production compared to the wild strain. This system can effectively reduce the optimizing costs, and is suitable for developing various types of RP expression hosts rapidly. KEY POINTS: • The basic growth-related resources can relieve the host burden from recombinant protein. • The enzyme-constrained model can accurately predict key genes to improve yield. • The expression intensity can be dynamically adjusted with changes in burden.


Assuntos
Proteínas de Escherichia coli , Escherichia coli , Proteínas de Transporte/genética , Escherichia coli/metabolismo , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Regiões Promotoras Genéticas , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo
20.
Environ Health ; 21(1): 84, 2022 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-36088422

RESUMO

BACKGROUND: We aimed to explore the association between long-term exposure to particulate matter ≤ 2.5 µm (PM2.5) and metabolic syndrome (MetS) and its components including fasting blood glucose (FBG), blood pressure, triglyceride (TG), high-density lipoprotein cholesterol (HDL-c) and waist circumference among adults and elderly in south China. METHODS: We surveyed 6628 participants in the chronic disease and risk factors surveillance conducted in 14 districts of Guangdong province in 2015. MetS was defined based on the recommendation by the Joint Interim Societies' criteria. We used the spatiotemporal land-use regression (LUR) model to estimate the two-year average exposure of ambient air pollutants (PM2.5, PM10, SO2, NO2, and O3) at individual levels. We recorded other covariates by using a structured questionnaire. Generalized linear mixed model was used for analysis. RESULTS: A 10-µg/m3 increase in the two-year mean PM2.5 exposure was associated with a higher risk of developing MetS [odd ratio (OR): 1.17, 95% confidence interval (CI): 1.01, 1.35], increased risk of fasting blood glucose level. (OR: 1.18, 95% CI: 1.02, 1.36), and hypertriglyceridemia (OR: 1.36, 95% CI: 1.18, 1.58) in the adjusted/unadjusted models (all P < 0.05). We found significant interaction between PM2.5 and the region, exercise on the high TG levels, and an interaction with the region, age, exercise and grain consumption on FBG (P interaction < 0.05). CONCLUSIONS: Long-term exposure to PM2.5 was associated with MetS, dyslipidemia and FBG impairment. Efforts should be made for environment improvement to reduce the burden of MetS-associated non-communicable disease.


Assuntos
Poluentes Atmosféricos , Síndrome Metabólica , Adulto , Idoso , Poluentes Atmosféricos/efeitos adversos , Glicemia/metabolismo , China/epidemiologia , Humanos , Síndrome Metabólica/complicações , Síndrome Metabólica/epidemiologia , Material Particulado/efeitos adversos , Triglicerídeos
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