Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 38
Filtrar
Filtros adicionais











Intervalo de ano
1.
Artigo em Inglês | MEDLINE | ID: mdl-31269513

RESUMO

PURPOSE: SUDOSCAN, a new non-invasive, quick, sensitive and quantitative technique, has been developed to detect diabetic peripheral neuropathy, and the latter is believed to be correlated with impaired ß-cell function. The purpose of the present study was to investigate the associations between ß-cell function indices and sudomotor function in Chinese type 2 diabetes. METHODS: A total of 266 Chinese patients with type 2 diabetes were enrolled. Sudomotor function was assessed using electrochemical skin conductance of hands and feet. Pancreatic ß-cell function was determined by homeostasis model assessment of ß-cell function index, early-phase ß-cell function indices and total ß-cell function indices. Pearson correlation analysis and multiple linear stepwise regression analysis were carried out to explore the associations between ß-cell function indices and sudomotor function. RESULTS: Patients with lower early-phase ß-cell function had lower electrochemical skin conductance levels of hands and feet and higher asymmetry ratio of hands and feet. Both Pearson correlation analysis and multiple linear stepwise regression analysis showed significantly positive relationships between early-phase ß-cell function and electrochemical skin conductance levels of hands and feet, after controlling for potential confounders (P<0.05). CONCLUSIONS: Impaired early-phase ß-cell function was positively associated with sudomotor dysfunction in Chinese patients with type 2 diabetes. We speculated that impaired early-phase ß-cell function may be associated with the incidence of sudomotor dysfunction in patients with T2DM.

2.
Medicine (Baltimore) ; 98(24): e16066, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31192969

RESUMO

To identify the value of predictors of poor prognosis of elderly patients with rectal cancer who underwent surgery, we investigated the relations between albumin to globulin ratio (AGR) and clinicopathological findings.We conducted a retrospective cohort study of clinicopathological characteristics (general status, pathological features of tumors, preoperative laboratory data, disease free, and overall survival) for elderly patients with stage I-III rectal cancer. The AGR is calculated as albumin/(total protein - albumin).According to the optimal cut-off point of AGR (1.43), the enrolled patients were divided into low AGR (n = 83) and high AGR (n = 136) groups. Meanwhile, age, hemoglobin, tumor size, and differentiation degree were the independent risk factors of low preoperative AGR value. Compared to patients with high AGR, those with low AGR were related to worse disease-free survival (DFS) (P = .0008) and overall survival (OS) (P = .0003). Moreover, in multivariate analysis, low AGR and poor TNM stage were the independent predictor of poor DFS and OS. Finally, the nomograms illustrated the effect of prognostic factors on DFS and OS.Preoperative AGR has a significant prognostic value and was identified as an independent predictor of DFS and OS in elderly rectal cancer patients.


Assuntos
Neoplasias Retais/sangue , Neoplasias Retais/cirurgia , Albumina Sérica/análise , Soroglobulinas/análise , Idoso , Idoso de 80 Anos ou mais , Biomarcadores Tumorais/sangue , Feminino , Seguimentos , Humanos , Masculino , Estadiamento de Neoplasias , Período Pré-Operatório , Prognóstico , Neoplasias Retais/mortalidade , Neoplasias Retais/patologia , Estudos Retrospectivos , Análise de Sobrevida , Carga Tumoral
3.
Medicine (Baltimore) ; 98(24): e16092, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31192972

RESUMO

BACKGROUND: Few studies have focused on octogenarian patients with gastric cancer (GC) who have undergone gastrectomy. This meta-analysis of published studies was performed to assess the safety of treating octogenarian GC patients with surgery. METHODS: Databases, including PubMed, Embase, Web of Science, and Cochrane Library were searched until January 2019. The incidence of preoperative comorbidities, postoperative complications, and mortality was assessed using odds ratios (ORs) with corresponding 95% confidence intervals (CIs). Further, the hazard ratios (HRs) with 95% CIs were applied for survival outcomes. RESULTS: A total of 18,179 patients with GC in 21 studies were included. Our results demonstrated that octogenarian patients were associated with a higher burden of comorbidities (OR = 2.79; 95% CI: 2.37, 3.28; P = .00), high incidences of overall postoperative complications (OR = 1.48; 95% CI: 1.22, 1.81; P = .00), medical postoperative complications (OR = 2.58; 95% CI: 1.91, 3.49; P = .00), in-hospital mortality (OR = 3.24; 95% CI: 2.43, 4.31; P = .00) and poor overall survival (HR = 1.96; 95% CI: 1.65, 2.27; P = .00). CONCLUSIONS: Considering the high burden of comorbidities, high incidences of postoperative complications and mortality, surgery for extremely elderly patients with GC requires deliberation. Individualized treatment is recommended for such patients.


Assuntos
Gastrectomia , Neoplasias Gástricas/cirurgia , Idoso de 80 Anos ou mais , Gastrectomia/efeitos adversos , Humanos , Segurança do Paciente , Complicações Pós-Operatórias/epidemiologia , Neoplasias Gástricas/epidemiologia
4.
Medicine (Baltimore) ; 98(19): e15663, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31083268

RESUMO

BACKGROUND: Recently, the incidence of proximal early gastric cancer (EGC) has been rising rapidly. Prevalent surgical methods are proximal gastrectomy (PG) and total gastrectomy (TG); however, which method is superior remains controversial. We conducted a systematic review and meta-analysis of original articles to compare the short- and long-term clinical outcomes of PG with TG for proximal EGC. METHODS: Databases, including PubMed, Embase, Web of Science, and Cochrane Library were searched up to October 2018. The Newcastle-Ottawa scale was utilized to conduct quality assessments, and publication bias was evaluated using Egger test. STATA version 14.0 was used to perform the meta-analysis. RESULTS: A total of 2036 patients with proximal EGC in 18 studies were included in the meta-analysis. The results showed that PG was potentially superior to TG regarding operation time, intraoperative blood loss volume, and long-term nutritional status. Overall survival between the PG and TG groups was not significantly different. PG was associated with a high incidence of 2 kinds of postoperative complications: anastomotic stenosis and reflux esophagitis. However, the incidence of these complications associated with esophagojejunostomy with double-tract reconstruction (DTR) was comparable with that of TG. CONCLUSIONS: PG has several advantages over TG for the treatment of proximal EGC, including surgical outcomes and long-term nutritional status. However, anastomotic stenosis and reflux esophagitis frequently occurred in patients undergoing PG. Esophagojejunostomy with DTR could offer a solution to reducing the incidence of these complications.


Assuntos
Gastrectomia/métodos , Neoplasias Gástricas/cirurgia , Humanos
5.
Eur J Pharmacol ; 852: 189-197, 2019 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-30876979

RESUMO

The bromodomain and extra-terminal domain (BET) protein BRD4 is emerging as a potential target for cancer therapy. However, BRD4 roles in regulating the stemness of gastric cancer cells are unclear. Here, we demonstrated that BRD4 expression was significantly increased in gastric cancer tissues, cell spheroids, and BRD4 knockdown attenuated the stemness of gastric cancer cells characterized as the decrease of stemness markers expression, capacity of cells spheroids formation and ALDH1 activity. Importantly, BRD4 expression was negatively correlated with overall survival, first progression survival and post progression survival of gastric cancer patients. Mechanistic investigations revealed that miR-216a-3p was the most remarkably upregulated miRNA in response to BRD4 knockdown and Wnt/ß-catenin signaling was necessary for BRD4-mediated promotion on the stemness of gastric cancer cells. Additionally, BRD4 directly bound to the promoter and promoted the methylation level of MIR216A promoter, thus decreasing miR-216a-3p level. Notably, Wnt3a was identified as the direct target of miR-216a-3p in gastric cancer cells. Therefore, our results defined a BRD4/miR-216a-3p/Wnt/ß-catenin pathway in regulating the stemness of gastric cancer cells.


Assuntos
MicroRNAs/genética , Células-Tronco Neoplásicas/patologia , Proteínas Nucleares/metabolismo , Neoplasias Gástricas/genética , Neoplasias Gástricas/patologia , Fatores de Transcrição/metabolismo , Via de Sinalização Wnt/genética , Sequência de Bases , Linhagem Celular Tumoral , Progressão da Doença , Técnicas de Silenciamento de Genes , Humanos , Proteínas Nucleares/deficiência , Proteínas Nucleares/genética , Fatores de Transcrição/deficiência , Fatores de Transcrição/genética
6.
J Sep Sci ; 42(5): 1088-1104, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30663861

RESUMO

Dendrobium huoshanense, a unique species in the genus Orchidaceae, is only found in China and is known as "mihu". Due to the lack of quality control, the use of D. huoshanense in the herbal market has been limited. In this study, methods based on thin-layer chromatography, high-performance liquid chromatography and high-performance liquid chromatography coupled with electrospray ionization multi-stage tandem mass spectrometry were used to identify the flavonoids in D. huoshanense and distinguish this species from other Dendrobium species. Using thin-layer chromatography, a characteristic band was observed for D. huoshanense, and this band was absent from the thin-layer chromatography plates of other Dendrobium species. Then, using high-performance liquid chromatography, nine peaks of flavonoids were observed in the chromatograms of ten batches of D. huoshanense. Ultimately, 22 flavonoids in D. huoshanense were identified by multi-stage tandem mass spectrometry, and 11 of these compounds are being reported from D. huoshanense for the first time. In addition, two compounds both with molecular weights of 710, were identified as being unique to D. huoshanense; one of these compounds, apigenin-6-C-α-L-rhamnosyl-(1→2)-ß-D-glucoside-8-C-α-L-arabinoside, was proven to be responsible for the characteristic thin-layer chromatography band of D. huoshanense. These analysis methods can be applied for the identification and quality control of D. Huoshanense.


Assuntos
Dendrobium/química , Flavonoides/análise , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Delgada , Dendrobium/classificação , Especificidade da Espécie , Espectrometria de Massas por Ionização por Electrospray , Espectrometria de Massas em Tandem
7.
Medicine (Baltimore) ; 98(4): e14235, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30681607

RESUMO

RATIONALE: Extramedullary plasmacytomas (EMP) are tumors composed by a monoclonal population of plasma cells that arise in extraosseus tissues, occupying <5% of all plasma cell neoplasms. Gastrointestinal solitary extramedullary plasmacytoma is rare, just comprises about 5% of all EMPs. The most common site is small intestine. The gastric incidence is much rare and especially the plasmablastic plasmacytoma in the stomach. PATIENT CONCERNS: A 65-year-old man had an epigastric discomfort and mass for about 2 months. Gastroscopy revealed a malignant tumor in the gastric body. Abdomen computed tomography (CT) showed that the gastric cavity was filled, and the irregular soft tissue shadow was seen in the greater curvature, and the enhancement was obvious. To get more tissue, we conducted stomach puncture biopsy. Pathology showed small-round cell malignant tumors. And immunohistochemical examinations revealed that the tumor tend to be a plasma cell tumor. DIAGNOSIS: Gastric plasma cell tumor. INTERVENTIONS: Distal gastrectomy was performed to treat the tumor. OUTCOMES: In addition to ascites caused by hypoproteinemia, there were no postoperative complications. Postoperative pathologic report showed plasmablastic plasmacytoma. Histopathologic examination of the specimen revealed plasmablastic plasmacytoma originating in the stomach with transmural extension, but without lymph node metastasis. The patient is regularly followed up at a postoperative clinic and is doing well, and at present there is no plan for adjuvant treatment. LESSONS: Surgical resection is good option for gastrointestinal EMP.


Assuntos
Plasmocitoma/patologia , Neoplasias Gástricas/patologia , Idoso , Humanos , Imunocompetência , Masculino , Plasmocitoma/imunologia , Neoplasias Gástricas/imunologia
8.
Medicine (Baltimore) ; 98(2): e14027, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30633195

RESUMO

BACKGROUND: Hepatic function is closely associated with prognosis in patients with hepatocellular cancer (HCC). In this study, a meta-analysis of the published studies was performed to assess the prognostic value of ALBI grade in HCC patients. METHODS: Databases, including PubMed, EMbase, Web of Science, and Cochrane Library were retrieved up to August 2018. The primary outcome was OS and secondary outcome was DFS, the prognostic impact of which was assessed by using hazard ratio (HRs) with corresponding 95% confidence intervals (CIs). The enrolled studies were analyzed by using STATA version 12.0 software. RESULTS: A total of 22,911 patients with HCC in 32 studies were included. Our results demonstrated that high pretreatment ALBI is associated with poor OS (HR = 1.719, 95%CI: 1.666-1.771, P = .000, univariate results; HR = 1.602, 95%CI: 1.470-1.735, P = .000, multivariate results) and poor DFS (HR = 1.411, 95%CI: 1.262-1.561, P = .000, univariate results; HR = 1.264, 95%CI: 1.042-1.485, P = .000, multivariate results). Meanwhile, when the analysis was stratified into subgroups, such as treatment methods, sample size, geographic area, and ALBI grade, the significant correlation in ALBI and poor long-term survival was not altered. CONCLUSION: High pretreatment ALBI is closely associated with poor prognosis in HCC, and High ALBI should be treated as an ideal predictor during hepatocellular therapy.


Assuntos
Bilirrubina/sangue , Carcinoma Hepatocelular/sangue , Neoplasias Hepáticas/sangue , Albumina Sérica , Biomarcadores Tumorais/sangue , Humanos , Prognóstico
9.
J Antibiot (Tokyo) ; 72(3): 164-168, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30560938

RESUMO

Two novel quinomycins I (1) and J (3) were discovered by UPLC-MS, then the two novel compounds and five known quinomycins A(2), B(4), E(5), C(6) and monosulfoxide quinomycin (7) were isolated from the culture broth of Streptomyces sp. HCCB11876. The structures of these compounds were elucidated through MS and NMR spectroscopic analysis. Compounds 1-7 showed significant antibacterial and cytotoxic activities. The structure-activity relationship indicated that sulfoxide group in N-methylcysteine of quinomycins (1, 3 and 7) would significantly decrease the antibacterial and cytotoxic activities. Moreover, the antibacterial and cytotoxic activities were decreased with the increase of carbon chain in amino-acid residues.


Assuntos
Antibacterianos/isolamento & purificação , Antineoplásicos/isolamento & purificação , Equinomicina/análogos & derivados , Streptomyces/metabolismo , Antibacterianos/química , Antibacterianos/farmacologia , Antineoplásicos/química , Antineoplásicos/farmacologia , Cromatografia Líquida de Alta Pressão , Meios de Cultura/química , Equinomicina/química , Equinomicina/isolamento & purificação , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Estrutura Molecular , Streptomyces/crescimento & desenvolvimento , Relação Estrutura-Atividade
10.
Biomed Pharmacother ; 108: 338-346, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30227327

RESUMO

This work aims to explore the roles and mechanisms of long non coding RNA (lncRNA) THOR in regulating the stemness of gastric cancer cells. RNA-sequencing combined with quantitative real-time PCR (qRT-PCR) indicated that lncRNA THOR level was significantly upregulated in gastric cancer tissues compared with that in normal adjacent tissues. Knockdown of THOR attenuated the stemnness of gastric cancer cells, evident by the decrease of stemness markers expression and capacity of cells spheroid formation. Further RNA-sequencing combined with qRT-PCR and western blot analysis demonstrated that expression of transcriptional factor SOX9 was remarkably decreased in gastric cancer cells with THOR stable knockdown. Additionally, RNA immunoprecipitation (RIP) combined with luciferase reporter assay revealed that THOR directly bound to SOX9 3' untranslated region (3'UTR), but not its 5'UTR or coding area. Notably, overexpression of SOX9 rescued THOR knockdown-mediated inhibition on the stemness of gastric cancer cells. Thus, our results suggest that THOR could potentiate the stemness of gastric cancer cells via directly binding to SOX9 3'UTR.

11.
Recent Pat Anticancer Drug Discov ; 13(4): 445-454, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29708076

RESUMO

BACKGROUND: Marine environment constitutes an almost infinite resource for novel anticancer drug discovery. The biodiversity of marine organisms provides a rich source for the discovery and development of novel anticancer peptides in the treatment of human cancer. Marine peptides represent a new opportunity to obtain lead compounds in biomedical field, particularly for cancer therapy. OBJECTIVE: Providing an insight of the recent progress of patented marine peptides and presenting information about the structures and mechanistic mode of anticancer activities of these marine peptides. METHODS: We reviewed recent progress on the patented anticancer peptides from marine organisms according to their targets on different signal pathways. This work focuses on relevant recent patents (2010-2018) that entail the anticancer activity with associated mechanism and related molecular diversity of marine peptides. The related cellular signaling pathways for novel peptides that induce apoptosis and affect tubulin-microtubule equilibrium, angiogenesis and kinase activity that are related to the anticancer and related pharmacological properties are also discussed. RESULTS: The recent patents (2010-2018) of marine peptides with anticancer activity were reviewed, and the anticancer activity of marine peptides with associated mechanism and related molecular diversity of marine peptides were also discussed. CONCLUSION: Marine peptides possess chemical diversity and display potent anticancer activity via targeting different signal pathways. Some of the marine peptides are promising to be developed as novel anticancer agents.

12.
Nanomedicine ; 14(3): 651-659, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29331387

RESUMO

Tumor-associated macrophages (TAMs) and angiogenesis are increasingly considered as the pivotal factors that affect tumor progress. Herein, we developed the paclitaxel (PTX)-loaded nanoparticles (NP/PTX) and decorated it with an innovative peptide YI (YINP/PTX) for simultaneously targeting delivery of drug to TAMs and angiogenesis. We demonstrated that the modification of YI peptide significantly enhanced the internalization of nanoparticles by cells and accumulation of nanoparticles in tumor tissues, but down regulated the distribution of them in normal tissues especially the liver. We also made a confirmation that the YI peptide decorated nanoparticles had an excellent co-localization with TAMs and angiogenesis in vivo. Finally, in the HT-26 colorectal tumor-bearing mice, a pharmacodynamic evaluation was performed and results showed that the YINP/PTX was more effective than other PTX formulations in anti-tumor growth. These results together suggested that the prepared nanoparticles are promising in targeting delivery of chemotherapeutics to tumor microenvironment for enhancing tumor therapy effect.

13.
Mini Rev Med Chem ; 18(12): 1035-1046, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29243575

RESUMO

BACKGROUND: Alzheimer's disease (AD) is one of the most devastating diseases worldwide. The current drugs for AD can only ameliorate the symptoms rather than reverse or prevent the progress of AD. On the other hand, blood-brain barrier (BBB), as a natural barrier, significantly impedes drug delivery from the blood circulation into the brain. Nanomedicine can be a safe, effective and promising approach to treat AD. OBJECTIVE: This review summarizes the recent nanomedicine research in this area, including the use of liposomes and nanoparticles (NPs), to provide new approach for targeted treatment of AD. METHOD: Collecting and referring to the related literature in recent 10 years, via searching MeSH Terms "Alzheimer's disease; nanomedicine; nanoparticle; amyloid ß peptide; tau protein; autophagy". RESULTS: Nanomedicines show superiority over conventional anti-AD drugs as a potential weapon against AD by the five proposed mechanisms: many unfavorable pharmaceutical properties of conventional anti-AD drugs maybe greatly overcome by nanomedicine; nanomedicines trigger efficient production of high-titer anti-Aß antibodies following controlled release of antigens by them; some apolipoprotein- based nanomedicines could preferably bind to Aß and increase the elimination of Aß nanomedicine-induced autophagy could be facilitated to increase the elimination of Aß nanomedicineinduced inhibition of tau aggregation. CONCLUSION: Therefore, nanomedicine-mediated drug therapy is promising in the treatment of AD.


Assuntos
Doença de Alzheimer/tratamento farmacológico , Nanomedicina , Doença de Alzheimer/metabolismo , Peptídeos beta-Amiloides/antagonistas & inibidores , Peptídeos beta-Amiloides/metabolismo , Animais , Humanos , Lipossomos/química , Nanopartículas/química , Agregados Proteicos/efeitos dos fármacos
14.
ACS Appl Mater Interfaces ; 9(46): 40614-40627, 2017 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-29095595

RESUMO

Matrix metalloproteinases (MMPs), mostly abundant in the tumor extracellular matrix (ECM), tumor cells, and tumor vasculatures, are closely correlated with tumor progression and metastasis. In this case, making use of MMPs was supposed to achieve site-specific drug delivery and a satisfactory tumor treatment effect. Herein, we rationally developed a novel tumor microenvironment and tumor cell dual-targeting nanoparticle by integrating a chemotherapeutic-loaded drug-loaded carrier and a versatile polypeptide-LinTT1-PVGLIG-TAT (LPT) which is composed of a multitargeting peptide-LinTT1 and a cell-penetrating peptide-TAT. The functionalized nanoparticles exhibited a superior affinity to A549 lung-cancer cells and microenvironment including angiogenesis and tumor-associated macrophages (TAMs) in our study. In addition, cellular experiments demonstrated that the cell-penetrating ability of TAT was significantly shielded by the addition of LinTT1 to the fourth lysine of the TAT via an MMP cleavable linker PVGLIG and could be recovered under the catalysis of MMPs. This design was supposed to efficiently decrease the toxicological risk to normal tissues induced by the unselectivity of TAT. The finally treatment effect investigation showed that tumor-bearing mice treated with LPT-modified nanoparticles achieved an enhanced efficacy for inhibiting tumor growth and the longest survival time as compared to other groups. Collectively, this study provides a novel robust nanoplatform which could simultaneously target the tumor microenvironment and tumor cell drug delivery for increasing the efficacy of cancer therapy.

15.
Gene Expr Patterns ; 25-26: 46-58, 2017 11.
Artigo em Inglês | MEDLINE | ID: mdl-28583421

RESUMO

Cumulus cells (CCs) are considered as an important source to predict oocyte quality. Despite numerous candidate genes in CCs have been identified for embryonic developmental competence, the results are inconsistent. The next generation RNA-sequencing was used to investigate the transcriptomic differences in CCs from in vitro matured oocytes did or did not develop to blastocyst stage following in vitro fertilization (IVF). In our study, the corresponding mouse oocytes were traced using a single-cell tracking system, and CCs were pooled into groups based on the embryonic developmental outcomes. In vivo matured oocytes with blastocyst development were set as a reference group. The transcriptomic differences in mouse CCs from in vitro maturated oocytes with or without blastocyst formation were tested by RNA-sequencing. Real-time PCR was used to verify the expression levels of those candidate genes. A total of 103 transcripts were significantly up-regulated, and 97 down-regulated, in the CCs with the oocytes developed to blastocyst stage. The bioinformatics study showed that those genes were involved in tube morphogenesis, cell-cell signaling and cell projection formation. Nine genes were selected from the most significantly changed transcripts after comparison with the reference group: Arrb1, Atp2c1, Cdh5, Cntnap1, Mkln1, Lgr4, Rhobtb1, Smc2 and Six2, as the candidate target genes. They were associated with the regulation of G-protein coupled receptors, Wnt and MAPK signaling, actin filaments and cell adhesion. Real-time PCR verified the up-regulation of all 9 genes, and significantly increased of Rhobtb1, Mkln1, Smc2, Arrb1, Atp2c1, Cdh5 and Lgr4. Based on RNA-sequencing, we found the changes in gene transcription of mouse CCs that were critical for the communication between CCs and oocytes. The results could provide novel insights on non-invasively predicting the oocyte quality and improving developmental competence.


Assuntos
Blastocisto/metabolismo , Rastreamento de Células/métodos , Células do Cúmulo/citologia , Perfilação da Expressão Gênica/métodos , Técnicas de Maturação in Vitro de Oócitos/métodos , Animais , Células do Cúmulo/química , Feminino , Regulação da Expressão Gênica no Desenvolvimento , Redes Reguladoras de Genes , Sequenciamento de Nucleotídeos em Larga Escala , Camundongos , Análise de Sequência de RNA , Transdução de Sinais , Análise de Célula Única
16.
J Periodontol ; 88(6): e97-e108, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28394188

RESUMO

BACKGROUND: Postoperative weight loss (POWL) is expected to occur in combined models of obesity and periodontitis. This study explores the confounding effects of POWL on the impact of ligation-induced periodontitis on glucose and lipid metabolism in obese animals. METHODS: Combined mouse models of diet-induced obesity (DIO) and ligation-induced periodontitis (5- or 10-day ligation) were studied. Fasting serum glucose (FSG), fasting insulin (Fins), and lipids including triglyceride (TG), total cholesterol (TC), and low- and high-density lipoprotein cholesterol (HDLC), were detected via biochemistry and enzyme-linked immunosorbent assay. POWL and homeostasis model assessment of insulin resistance (HOMA-IR) were calculated. Analysis of covariance was performed to identify confounding effects of POWL. RESULTS: The obesity, periodontitis, and 10-day groups exhibited greater POWL than corresponding controls (P <0.01). Without considering POWL, conflicting results were found, including: 1) contradictory changes in HDLC caused by obesity or periodontitis; and 2) unequal levels of FSG, TC, and HDLC between days 5 and 10 in the sham-ligation controls. Moreover, upregulating effects of periodontitis were found only on TG in the DIO mice, whereas those on Fins, HOMA-IR, and HDLC were statistically veiled. After the confounding effects of POWL were filtered, periodontitis promoted increased levels of not only TG but also Fins, HOMA-IR, and HDLC in the DIO mice (P <0.05). CONCLUSIONS: When analyzing the interrelationship between obesity and periodontitis, the confounding effects of an imbalanced POWL should be considered. Otherwise, impact of periodontitis on metabolic dysregulation in obese animals may be underestimated.


Assuntos
Obesidade/complicações , Obesidade/metabolismo , Periodontite/complicações , Periodontite/metabolismo , Perda de Peso , Perda do Osso Alveolar , Animais , Glicemia/análise , Colesterol/sangue , HDL-Colesterol , LDL-Colesterol/sangue , Citocinas/sangue , Dieta , Modelos Animais de Doenças , Jejum , Feminino , Glucose/metabolismo , Insulina/sangue , Resistência à Insulina , Metabolismo dos Lipídeos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Obesos , Obesidade/sangue , Periodontite/sangue , Periodontite/patologia , Triglicerídeos/sangue
17.
Methods Mol Biol ; 1568: 33-44, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28421487

RESUMO

Current freezing technology, especially the vitrification method, has markedly improved oocyte survival rate after warming, and the pregnancy rate is comparable to that achieved with fresh oocytes. However, most groups report using oocytes matured in vivo for vitrification. Although immature oocytes can be vitrified successfully, clinical outcomes do not reach that of vitrification of matured oocytes. The current literature suggests that oocytes should be vitrified at mature metaphase II (M-II) stage following IVM rather than at the immature germinal vesicle (GV) stage, because the potential for oocyte maturation is reduced when vitrification is performed on immature oocytes at the GV stage.


Assuntos
Criopreservação/métodos , Oócitos/citologia , Animais , Diferenciação Celular , Humanos , Oogênese , Vitrificação
18.
J Colloid Interface Sci ; 497: 333-342, 2017 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-28301829

RESUMO

Two N3-substituted imidazoles 1,2-dimethylimidazole and 1-butyl-2-methylimidazole were chosen to functionalize poly(aryl ether ketone), respectively. The generated imidazolium cations could electrostatically react with sulfonate ions of the sulfonated poly(ether ether ketone) forming the ionic crosslinking structure of the membranes. The changes in crosslinking degree and the alkyl chain-length on N3 site of the imidazoliums could highly affect the properties of the anion exchange membranes (AEMs). The AEMs functionalized by 1-butyl-2-methylimidazole exhibited superior properties compared to those functionalized by 1,2-dimethylimidazole according to the tolerance tests of the AEMs towards hot alkaline solutions. After exposed to 1M KOH at 80°C for 200h, the 1-butyl-2-methylimidazole modified AEMs maintained the ion exchange capacity of above 85%, the conductivity of about 70%, and the tensile stress at break of around 80%, respectively. The hydrophile-lipophile balance of the polymer membranes was calculated and proposed to better understand the correlation between structures and properties of the AEMs. The degradation of the imidazolium functional groups of the AEMs under the attack of hydroxide ions was evidenced by FT-IR analysis.

19.
PLoS One ; 12(2): e0171452, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28182716

RESUMO

Lysozyme-like proteins (LYZLs) belong to the c-type lysozyme/α-lactalbumin family and are selectively expressed in the mammalian male reproductive tract. Two members, human sperm lysozyme-like protein (SLLP) -1 and mouse LYZL4, have been reported to contribute to fertilization but show no bacteriolytic activity. Here, we focused on the possible contribution of LYZL6 to immunity and fertilization. In humans, LYZL6 was selectively expressed by the testis and epididymis and became concentrated on spermatozoa. Native LYZL6 isolated from sperm extracts exhibited bacteriolytic activity against Micrococcus lysodeikticus. Recombinant LYZL6 (rLYZL6) reached its peak activity at pH 5.6 and 15 mM of Na+, and could inhibit the growth of Gram-positive, but not Gram-negative bacteria. Nevertheless, the bacteriolytic activity of rLYZL6 proved to be much lower than that of human lysozyme under physiological conditions. Immunodetection with a specific antiserum localized the LYZL6 protein on the postacrosomal membrane of mature spermatozoa. Immunoneutralization of LYZL6 significantly decreased the numbers of human spermatozoa fused with zona-free hamster eggs in a dose-dependent manner in vitro. Thus, we report here for the first time that LYZL6, an acidic, bacteriolytic and human sperm-related protein, is likely important for fertilization but not for the innate immunity of the male reproductive tract.


Assuntos
Fertilização/genética , Muramidase/fisiologia , Espermatozoides/metabolismo , Sequência de Aminoácidos , Animais , Bacteriólise/genética , Cricetinae , Feminino , Humanos , Masculino , Camundongos , Muramidase/química , Muramidase/genética , Coelhos , Interações Espermatozoide-Óvulo/genética , Testículo/metabolismo
20.
Zhonghua Nan Ke Xue ; 23(1): 3-10, 2017 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-29658230

RESUMO

Objective: To prepare a polyclonal antibody against human lysozyme-like protein 4 (LYZL4) expressed in the prokaryotic system and identify the distribution of LYZL4 in the testis. METHODS: The full-length cDNA of LYZL4 was cloned into the pET32a plasmid and the expression of the recombinant LYZL4 (rLYZL4) was induced by IPTG. The rLYZL4 was purified by Ni-NTA and chitin affinity chromatography respectively and its bactericidal activity was observed by bilayer agar plate diffusion assay. The purified rLYZL4 was used as an immunogen to generate the polyclonal antibody, followed by examination of the antibody titer by ELISA and its specificity by Western blot. The distribution of LYZL4 in human tissue, sperm and seminal plasma was identified and its subcellular localization in the testis was determined by immunohistochemistry. RESULTS: rLYZL4 was expressed efficiently in the prokaryotic system and exhibited no bacteriolytic activity against M. lysodeikticus and E. coli. The anti-rLYZL4 polyclonal antibody could bind the recombinant protein with a high sensitivity and specificity. LYZL4 was identified in the testis, epididymis and sperm protein extracts and localized in the acrosomal region of round and elongating spermatids. CONCLUSIONS: An anti-rLYZL4 polyclonal antibody was successfully prepared using the prokaryotic expression system. LYZL4 was detected in the acrosomal region of round and elongating spermatids, suggesting an association with the structure and function of the acrosome.


Assuntos
Anticorpos/análise , Muramidase/imunologia , Testículo/imunologia , Acrossomo/imunologia , Animais , Western Blotting , DNA Complementar , Ensaio de Imunoadsorção Enzimática , Epididimo/imunologia , Escherichia coli , Humanos , Imuno-Histoquímica , Masculino , Muramidase/genética , Plasmídeos , Proteínas Recombinantes/genética , Sêmen/imunologia , Espermatozoides/imunologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA