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1.
Cereb Cortex ; 2020 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-32219328

RESUMO

Synaptic plasticity is the neural basis of physiological processes involved in learning and memory. Tripartite motif-containing 32 (TRIM32) has been found to play many important roles in the brain such as neural stem cell proliferation, neurogenesis, inhibition of nerve proliferation, and apoptosis. TRIM32 has been linked to several nervous system diseases including autism spectrum disorder, depression, anxiety, and Alzheimer's disease. However, the role of TRIM32 in regulating the mechanism of synaptic plasticity is still unknown. Our electrophysiological studies using hippocampal slices revealed that long-term potentiation of CA1 synapses was impaired in TRIM32 deficient (KO) mice. Further research found that dendritic spines density, AMPA receptors, and synaptic plasticity-related proteins were also reduced. NMDA receptors were upregulated whereas GABA receptors were downregulated in TRIM32 deficient mice, explaining the imbalance in excitatory and inhibitory neurotransmission. This caused overexcitation leading to decreased neuronal numbers in the hippocampus and cortex. In summary, this study provides this maiden evidence on the synaptic plasticity changes of TRIM32 deficiency in the brain and proposes that TRIM32 relates the notch signaling pathway and its related mechanisms contribute to this deficit.

2.
ChemSusChem ; 2020 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-32118355

RESUMO

The valorization of lignin to replace phenol is significant in the production of phenolic resins. However, a great challenge is to produce lignin-based resin (LR) with a suitable viscosity and high substitution rate of lignin to phenol. In this study, LRs were produced using hardwood technical lignin derived from the pulping industry. Structural analysis of the LRs indicated that the unsubstituted para and ortho carbon atoms of the aromatic ring influenced the curing temperature and activation energy of the resins. The curing kinetics and thermal decomposition study implied that urea and methylene groups in cured LRs were significant factors that affected the thermal stability negatively. The prepared LRs showed desirable features if used as adhesives to make plywood. This is the first approach in which a substitution rate of up to 65 % is achieved for low-reactive-site hardwood lignin, which provides a solution to the challenge of the simultaneous realization of the high addition of lignin and the adaptive viscosity of resins.

3.
J Asian Nat Prod Res ; : 1-15, 2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-32024387

RESUMO

We described the design, synthesis and antimicrobial activities of novel pleuromutilin derivatives with substituted piperazine substrate. Minimum inhibitory concentration (MIC) was used to evaluate the activity of the derivatives against six bacteria in vitro, and compound 8 was potent against Staphylococcus aureus and Staphylococcus epidermidis with the MIC value of 0.0625 µg/ml. 10a and 10 b showed similar activity to positive control drugs (tiamulin, erythromycin) against S. aureus with the MIC value of 0.125 µg/ml. The binding mode of compound 8 and tiamulin to the ribosome pocket showed the correlation between binding parameters and the antibacterial activity, and more bonds and stronger combination could effectively enhance the activity of compounds.

4.
Plant Dis ; 104(4): 1231-1238, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32065563

RESUMO

Powdery mildew, which is caused by Blumeria graminis f. sp. tritici (Bgt), is a disease of wheat worldwide. Xiaobaidong is a Chinese wheat landrace, which still maintains good resistance against powdery mildew. To obtain more genetic markers closely linked to the powdery mildew resistance gene mlxbd and narrow the candidate region for its isolation, new simple sequence repeats and cross intron-spanning markers were designed based on the genome sequence of Triticum aestivum cultivar Chinese Spring chromosome 7BL. The flanking markers 7BLSSR49 and WGGC5746 were found to be tightly linked to mlxbd at genetic distances of 0.4 cM and 0.3 cM, respectively. The resistance locus was mapped to a 63.40 kb and 0.29 Mb region of the Chinese Spring genome and Zavitan genome, respectively. The linked markers of mlxbd could be used as diagnostic markers for mlxbd. The linked molecular markers and delineated genomic region in the sequenced Chinese Spring genome will assist the future map-based cloning of mlxbd.

5.
Insect Biochem Mol Biol ; 120: 103337, 2020 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-32109588

RESUMO

As the counterparts of noradrenaline and adrenaline in vertebrates, octopamine (OA) regulates multiple physiological and behavioral processes in invertebrate. OA mediates its effects via binding to specific octopamine receptors (OARs). Functional and pharmacological characterization of OARs have been reported in several insects. However, little work was documented in hemipteran insects. We cloned a ß-adrenergic-like OAR (NcOA2B2) from Nephotettix cincticeps. NcOA2B2 shares high similarity with members of the OA2B2 receptor class. Transcript level of NcOA2B2 varied in various tissues and was highly expressed in the leg. After heterologous expression in CHO-K1 cells, NcOA2B2 was dose-dependently activated by OA (EC50 = 2.56 nM) and tyramine (TA) (EC50 = 149 nM). Besides putative octopaminergic agonists, dopaminergic agonists and amitraz and DPMF potently activated NcOA2B2 in a dose-dependent manner. Receptor activity was blocked by potential antagonists and was most efficiently antagonized by asenapine. Phentolamine showed both antagonist and agonist effects on NcOA2B2. Our results offer the important information about molecular and pharmacological characterization of an OAR from N. cincticeps that will provide the basis for forthcoming studies on its roles in physiological processes and behaviors, and facilitate the design of novel insecticides for pest control.

6.
J Dairy Res ; 87(1): 103-109, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32008586

RESUMO

We compared the electrical conductivity from two different aggregates of whey protein concentrates (WPC) film: conventional amorphous aggregation at natural pH (pH 6.5) and amyloid fibrils at a low pH (pH 2.0) far away from the isoelectric point. The two types of film fabricated by these solutions with different aggregate structures showed large variations in electrical conductivity and other properties. The WPC fibril film (pH 2.0) exhibited higher electrical conductivity than that of the conventional WPC film (pH 6.5), improved mechanical properties and oil resistance, due to varying morphology, higher surface hydrophobicity and more (absolute value) surface charge of film-forming solutions. The evidence from this study suggests that fibrilized WPC with high-ordered and ß-sheets-rich structures fabricated high electrical conductivity film, which broadens the potential application of fibrils as functional bio-nanomaterials.

7.
Carbohydr Polym ; 233: 115860, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32059911

RESUMO

A novel multiple active sites cellulose-based adsorbent (MCC/TEPAA-BCTTC) with a high density of multiple active adsorption sites (N, O, S) was prepared by using epichlorohydrin cross-linking microcrystalline cellulose (MCC) with tetraethylenepentamine (TEPA), followed by grafting with bis(carboxymethyl) trithiocarbonate (BCTTC). It was shown that the removal rates of MCC/TEPAA-BCTTC for Pb(II) (1 mg/L), Cu(II) (3 mg/L) and Cr(VI) (1 mg/L) reached 100 %, 98 % and 99 %, respectively, and the remaining concentration after adsorption reached the United States Environmental Protection Agency (US EPA) standards for Pb(II) and Cu(II) and the China integrated wastewater discharge standard for Cr(VI). These results indicate that the high removal rate of MCC/TEPAA-BCTTC for removing anionic and cationic heavy metal ions in low-concentration mixed heavy metal ions environments was mainly due to the high density of multiple adsorption sites that act via multiple cooperative mechanisms.

8.
Nanoscale ; 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-32016265

RESUMO

Organic phase change materials (OPCMs) play a great role in energy management owing to their large phase change enthalpy, but their intrinsic low thermal conductivity (TC) and bad encapsulation severely restrict their applications. To overcome these problems, we developed a novel but feasible method to fabricate a graphene nanoplatelet (GNP) aerogel with compact and oriented stacking in-plane walls and many through-plane bridges via melamine foam (MF) and cellulose nanofiber (CNF) co-mediated assembly of GNPs. After impregnating paraffin wax (PW), the composite PCMs exhibit a high TC of 1.42 W m-1 K-1 at only a GNP content of 4.1 wt%, increasing by 407% compared with pure PW, and simultaneously nearly no reduction of the phase change enthalpy of PW. Meanwhile, this kind of composite PCM can not only show excellent light-to-thermal and electric-to-thermal transition ability, but also be applied in delay switch devices with satisfactory results.

9.
Dalton Trans ; 2020 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-32073112

RESUMO

A low-valent magnesium(i) complex [(XylNacnac)Mg]2 was employed as a highly efficient precatalyst for the hydroboration of a variety of cyclic and linear organic carbonates, polycarbonates, CO2 and esters with HBpin under mild conditions. The resultant boronates can be used for the preparation of the corresponding value-added diols, triols or alcohols through hydrolysis.

10.
Nat Commun ; 11(1): 323, 2020 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-31949152

RESUMO

The two-dimensional (2D) Ruddlesden-Popper organic-inorganic halide perovskites such as (2D)-phenethylammonium lead iodide (2D-PEPI) have layered structure that resembles multiple quantum wells (MQW). The heavy atoms in 2D-PEPI contribute a large spin-orbit coupling that influences the electronic band structure. Upon breaking the inversion symmetry, a spin splitting ('Rashba splitting') occurs in the electronic bands. We have studied the spin splitting in 2D-PEPI single crystals using the circular photogalvanic effect (CPGE). We confirm the existence of Rashba splitting at the electronic band extrema of 35±10 meV, and identify the main inversion symmetry breaking direction perpendicular to the MQW planes. The CPGE action spectrum above the bandgap reveals spin-polarized photocurrent generated by ultrafast relaxation of excited photocarriers separated in momentum space. Whereas the helicity dependent photocurrent with below-gap excitation is due to spin-galvanic effect of the ionized spin-polarized excitons, where spin polarization occurs in the spin-split bands due to asymmetric spin-flip.

11.
Org Biomol Chem ; 18(4): 660-665, 2020 01 28.
Artigo em Inglês | MEDLINE | ID: mdl-31894798

RESUMO

We have developed a mild and direct dimerization of dihydropyrrolo[2,1-a]isoquinolines through FeCl3-mediated oxidative homocoupling. A series of dimeric dihydropyrroloisoquinoline derivatives were obtained in acceptable to excellent yields (up to >99%). Attempts to expand this dimerization system to the synthesis of dimeric pyrroles failed and chlorination products were obtained in most cases instead of dimeric pyrroles.

12.
Int Immunopharmacol ; 79: 106175, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31918060

RESUMO

Acute lung injury (ALI) is one of the severe complications in patients with traumatic brain injury (TBI), contributing to the high mortality. Ghrelin has protective effects against various inflammatory diseases, but the effects of Ghrelin on TBI-induced ALI and its mechanisms remain unknown. In this study, Ghrelin administration was performed on the mice with TBI, then histological change in cortex and lung tissues, lung vascular permeability and macrophage number in bronchoalveolar lavage fluid (BALF) were examined, respectively. Simultaneously, the alterations of proinflammatory factors and pyroptosis-related proteins in lung tissues were detected. As a result, TBI-induced ALI was ameliorated after Ghrelin treatment, which was demonstrated by improved histology, reduced lung vascular permeability, and peripheral macrophage number. Furthermore, Ghrelin decreased the mRNA levels of proinflammatory factors (IL-1ß, IL-6, TNF-α and IL-18), the protein levels of pyroptosis-related proteins (NLRP3, Caspase1-P20, HMGB1 and Gasdermin D), and the phosphorylation levels of NF-κB in lung tissues. These results showed that Ghrelin attenuating TBI-induced ALI might be via ameliorating inflammasome-induced pyroptosis by blocking NF-κB signal, which are important for the prevention and treatment of TBI-induced ALI.

13.
Orthop Surg ; 12(1): 277-285, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31916393

RESUMO

OBJECTIVE: To investigate whether miR-141 and the sex determination region of Y chromosome box 11 (SOX11) play roles in steroid-induced avascular necrosis of the femoral head (SANFH), and to explore whether miR-141 could target SOX11 to influence the proliferation of bone marrow mesenchymal stem cells (BMSC). METHODS: Bone marrow mesenchymal stem cells (BMSC) were isolated and cultured from 4-week-old Sprague Dawley rats. A flow cytometry assay was performed to identify BMSC. BMSC were divided into two groups: a control group and a dexamethasone (DEX) group. BMSC were transfected by miR-141 mimic, miR-141 inhibitor, and SOX11. Real-time polymerase chain reaction (PCR) assay was performed to investigate the mRNA expression of miR-141 and SOX11. The results were used to determine the effect of transfection and to verify the expression in each group and the association between miR-141 and SOX11. Luciferase reporter assay revealed the targeted binding site between miR-141 and the 3'-untranslated region of SOX11 mRNA. MTT assays were performed to investigate the proliferation of BMSC in the miR-141 mimic, miR-141 inhibitor, and SOX11 groups. RESULT: The results of the flow cytometry assay suggested that cells were positive for CD29 and CD90 while negative for CD45. This meant that the isolated and cultured cells were not hematopoietic stem cells. In addition, cell transfection was successful based on the expression of miR-141 and SOX11. According to the results of real-time PCR assay, the mRNA expression of miR-141 in SANFH was upregulated (4.117 ± 0.042 vs 1 ± 0.027, P < 0.001), while SOX11 was downregulated (0.611 ± 0.055 vs 1 ± 0.027, P < 0.001) compared with the control group. Based on the results of the luciferase experiment, MiR-141 could directly target the expression of SOX11. Inhibition of miR-141 could upregulate the expression of SOX11 (2.623 ± 0.220 vs 1 ± 0.095, P < 0.001) according to the results of a real-time PCR assay. MiR-141 inhibited the proliferation of BMSC (0.618 ± 0.092 vs 1.004 ± 0.082, P < 0.001), while suppression of miR-141 increased the proliferation of BMSC (0.960 ± 0.095 vs 0.742 ± 0.091, P < 0.001). Furthermore, according to the results of the MTT assay, SOX11 promoted the proliferation of BMSC (1.064 ± 0.093 vs 0.747 ± 0.090, P < 0.001). CONCLUSION: MiR-141 inhibited the proliferation of BMSC in SANFH by targeting SOX11. Inhibition of miR-141 upregulated the expression of SOX11 and promoted the proliferation of BMSC. MiR-141 and SOX11 could be new targets for investigating the mechanism of SANFH.

14.
Hum Gene Ther ; 2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-31992084

RESUMO

The pro-renin receptor (PRR) is an important novel component of the renin-angiotensin (Ang) system that has multiple functions, which are not yet completely understood. In this study, we aimed to explore the effect of PRR on the formation of Ang II-induced abdominal aortic aneurysm (AAA) in apolipoprotein E-knockout mice. We used Ang II (1.44 mg/kg/day) infusion to induce AAA followed by a treatment of saline, telmisartan, no treatment, Ad-EGFP, Ad-PRR, or Ad-PRR plus telmisartan. The incidence of AAA was 35%, 60%, 65%, 90%, and 55% in the Telmisartan, Vehicle, Ad-EGFP, Ad-PRR, and Ad-PRR+Telmisartan groups, respectively. Compared with the Vehicle and Ad-EGFP groups, PRR overexpression markedly increased macrophage infiltration; levels of proinflammatory cytokines, including monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-α (TNF-α); the expression and activity of MMP2 and MMP9; NOX2 and NOX4 protein and mRNA expression; nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity; extracellular-signal-regulated kinase (ERK) and P38MAPK expression; but decreased smooth muscle cells content in AAA. However, telmisartan reversed the adverse effects of PRR. In addition, ERK inhibitor PD98059 eliminated the acceleration of Ang II-induced AAA formation by PRR, and coadministration of telmisartan and PD98059 further abolished the adverse effects of PRR on Ang II-induced AAA formation. Thus, PRR plays an important role in the pathological development of AAA via both Ang II-dependent and Ang II-independent activation of ERK pathways. These results suggest that inhibition of PRR activation may be a promising approach to the treatment of AAA.

15.
Vet Microbiol ; 240: 108542, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31902499

RESUMO

Influenza A virus (IAV) and bacteria co-infection can influence the host clinical conditions. Both H9N2 IAV and Pseudomonas aeruginosa (P. aeruginosa) are potential pathogens of respiratory diseases in mink. In this study, to clarify the effects of H9N2 IAV and P. aeruginosa co-infections on hemorrhagic pneumonia in mink, we carried out to establish the mink models of the two-pathogen co-infections in different orders. Compared with the single infections with H9N2 IAV or P. aeruginosa, the mink co-infected with H9N2 IAV and P. aeruginosa showed severe respiratory diseases, and exacerbated histopathological lesions and more obvious apoptosis in the lung tissues. H9N2 IAV shedding and viral loads in the lungs of the mink co-infected with H9N2 IAV and P. aeruginosa were higher than those in the mink with single H9N2 IAV infection. Furthermore, the clearance of P. aeruginosa in the co-infected mink lungs was delayed. In addition, the anti-H9N2 antibody titers in mink with P. aeruginosa co-infection following H9N2 IAV infection were significantly higher than those of the other groups. This implied that H9N2 IAV and P. aeruginosa co-infection contributed to the development of hemorrhagic pneumonia in mink, and that P. aeruginosa should play a major role in the disease. The exact interaction mechanism among H9N2 IAV, P. aeruginosa and the host needs to be further investigated.

16.
Bioresour Technol ; 297: 122471, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31787511

RESUMO

Hereon, tobacco stalk was deconstructed by lyophilization, ball-milling, ultrasound-assisted alkali extraction, hydrothermal pretreatment (HTP), and alkali presoaking, respectively, followed by dilute alkali cooking to both improve its enzymatic digestibility and isolate the hemicellulosic streams. It was found that a maximum cellulose saccharification rate of 93.5% was achieved from the integrated substrate by ball-milling and dilute alkali cooking, which was 4.4-fold higher than that from the raw material. Interestingly, in this case, 76.9% of hemicelluloses were simultaneously recovered during the integrated treatment. Structural determination indicated that the hemicelluloses released from tobacco stalk by dilute alkali cooking were mixed polysaccharides, and the (1 â†’ 4)-linked ß-D-Xylp backbone branched with L-Araf units at O-2/O-3 and 4-O-Me-α-D-GlcpA units at O-2 of the xylose residues was the main structure. In comparison, ultrasound-assisted alkali extraction, ball-milling, and HTP favored the extraction of hemicelluloses with less branched structure and lower molecular weights in the following alkali cooking.


Assuntos
Celulose , Tabaco , Hidrólise , Peso Molecular , Polissacarídeos
17.
J Cell Biochem ; 121(1): 385-393, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31222822

RESUMO

In this study, we aimed to study the effect of miR-33b in regulating sensitivity to daunorubicin (DNR) in acute myelocytic leukemia (AML). We used quantitative real-time polymerase chain reaction and Cell Counting Kit-8 assay to detect the level of miR-33b and cell viability. Cell apoptosis and the expression of eIF5A-2 and MCL-1 protein were detected by flow cytometry analysis and Western Blot analysis, respectively. MiR-33b mimic increased sensitivity of AML cells against DNR, while miR-33b inhibitor had the opposite effect. Furthermore, the results showed that the eIF5A-2 gene was a direct target of miR-33b, and miR-33b regulated eIF5A-2 mRNA and protein expression. Silencing of eIF5A-2 by RNA interference increased the sensitivity of AML cells against DNR. We also found that MCL-1 contributed to the regulation of DNR sensitivity, which was dependent on downregulation of eIF5A-2. Finally, knockdown of eIF5A-2 eliminated the effects of miRNA-33b mimic or inhibitor on DNR sensitivity. These findings indicate that miR-33b maybe as a new therapeutic target in AML cells.

18.
Shanghai Kou Qiang Yi Xue ; 28(4): 378-383, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31792477

RESUMO

PURPOSE: To prepare a soft lining material with high elastic on the basis of excellent characteristics of Eucommia ulmoides gum, and explore its basic physical performance and cytotoxity. METHODS: Basic formula was used based on the existing formula of elastic Eucommia ulmoides gum and medical rubber, then hardness, tensile strength and elongation at break were investigated to determine the final formula of the material, named Eucommia ulmoides gum composite soft denture liner. L929 mouse fibroblasts cultured in vitro were used to detect the toxic effects of 50% of leaching solution released from three kinds of denture soft lining materials by MTT assay. Statistical analysis was performed using SPSS16.0 software package. RESULTS: The kinds and blending proportion of rubber matrix had significant impact on mechanical properties of Eucommia ulmoides gum composite soft denture liner, the addition of Eucommia ulmoides gum significantly increased the hardness, tensile strength and elongation at break of the butadiene rubber. MTT assay showed Eucommia ulmoides gum composite soft denture liner did not have cytotoxicity on L929 mouse fibroblasts. The toxicity levels of 5 groups of Eucommia ulmoides gum composite soft denture liner in the 2nd, 4th, 7th day were maintained in the first stage, only 70:30 group reached the second stage on the 7th day. The cell relative appreciation rate of Silagum and 5 groups of Eucommia ulmoides gum composite soft denture liner in 2nd, 4th, 7th day were significantly higher than that in the self-curing acrylic soft lining material (P<0.05). CONCLUSIONS: Eucommia ulmoides gum and butadiene rubber blend ratio of 70:30, adding appropriate amount of zinc oxide, magnesium, stearic acid, sublimed sulfur, CZ, antioxidants, can get optimum mechanical properties. Eucommia ulmoides gum composite soft denture liner has no effect on L929 fibroblasts proliferation, indicating good biocompatibility.


Assuntos
Reembasadores de Dentadura , Eucommiaceae , Animais , Materiais Dentários , Bases de Dentadura , Dureza , Teste de Materiais , Camundongos , Resistência à Tração
19.
Am J Transl Res ; 11(11): 6989-6999, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31814902

RESUMO

Recent evidence has shown that long noncoding RNAs (lncRNAs) play major roles in tumorigenesis and cancer progression. The cancer genome atlas program (TCGA) database was used to screen colon adenocarcinoma (COAD)-related differentially expressed lncRNAs, which revealed that lncRNA ELFN1-AS1 was highly expressed in COAD. This study aimed to explore the regulatory role of ELFN1-AS1 in COAD and construct a gene delivery system based on extracellular vesicles (EVs). We found that ELFN1-AS1 levels were obviously increased in COAD patients and COAD tumor cells. Knockdown of ELFN1-AS1 expression by siRNA inhibited COAD cell proliferation and migration. Moreover, silencing ELFN1-AS1 significantly reduced the activation of extracellular signal-regulated protein kinase (Erk), up-regulated the protein expression of E-cadherin and down-regulated vimentin. In addition, we treated human umbilical cord mesenchymal stem cells (hUCMSCs) with siRNA-ELFN1-AS1 and found that EVs from siRNA-ELFN1-AS1-treated hUCMSCs could inhibit COAD cell proliferation and migration in vitro. These findings suggested that ELFN1-AS1 could promote the progression of COAD and that hUCMSC-EVs might be an attractive vehicle for the clinical administration of lncRNA-specific siRNAs in patients with COAD.

20.
Parasit Vectors ; 12(1): 592, 2019 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-31852494

RESUMO

BACKGROUND: Eimeria tenella is a highly pathogenic coccidian that causes avian coccidiosis. Both nitromezuril (NZL) and ethanamizuril (EZL) are novel triazine compounds with high anticoccidial activity, but the mechanisms of their action are still unclear. This study explored the response of E. tenella to NZL and EZL by the study of changes in protein composition of the second-generation merozoites. METHODS: Label-free quantification (LFQ) proteomics of the second-generation merozoites of E. tenella following NZL and EZL treatment were studied by LC-MS/MS to explore the mechanisms of action. The identified proteins were annotated and analyzed by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and protein-protein interaction (PPI) networks analysis. RESULTS: A total of 1430 proteins were identified by LC-MS/MS, of which 375 were considered as differential proteins in response to drug treatment (DPs). There were 26 only found in the NZL treatment group (N-group), 63 exclusive to the EZL treatment group (E-group), and 80 proteins were present in both drug groups. In addition, among the DPs, the abundant proteins with significantly altered expression in response to drug treatment (SDPs) were found compared with the C-group, of which 49 were upregulated and 51 were downregulated in the N-group, and 66 upregulated and 79 downregulated in the E-group. Many upregulated proteins after drug treatment were involved in transcription and protein metabolism, and surface antigen proteins (SAGs) were among the largest proportion of the downregulated SDPs. Results showed the top two enriched GO terms and the top one enriched pathway treated with EZL and NZL were related, which indicated that these two compounds had similar modes of action. CONCLUSIONS: LFQ proteomic analysis is a feasible method for screening drug-related proteins. Drug treatment affected transcription and protein metabolism, and SAGs were also affected significantly. This study provided new insights into the effects of triazine anticoccidials against E. tenella.

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