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1.
J Hazard Mater ; 398: 122997, 2020 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-32512460

RESUMO

As a natural radionuclide, uranium (U) has obvious phytotoxicity, the purpose of this study is to unravel the response mechanism of U on photosynthetic and respiratory metabolism in plants. Therefore, 14-day-old Vicia faba seedlings were exposed to 0-25 µM U during 72 h. U effects on growth parameters, physiological parameters of plants, and potential phytotoxicity mechanism were investigated by physiological analysis, and metabolome and transcriptome data. U significantly inhibited photosynthesis and respiration of plants. In metabolome analysis, 53 metabolites related to carbohydrate metabolism were identified (13 up-regulated, 12 down-regulated). In transcriptome analysis, U significantly inhibited the expression of photoreactive electron transport chain (up: 0; down: 31), Calvin cycle (up: 0; down: 12) and photorespiration pathway genes (up: 0; down: 8). U significantly inhibited the expression of cellular energy metabolic pathways genes (e.g., glycolysis, TCA cycle, and oxidative phosphorylation pathways) (up 8, down 18). We concluded that U inhibited the expression of genes involved in the photosynthetic metabolic pathway, which caused the decrease of photosynthetic rate. Meanwhile, U inhibited the expression of the electron transport chain genes in the mitochondrial oxidative phosphorylation pathway, which leads to the abnormal energy supply of cells and the inhibition of root respiration rate.

2.
Ying Yong Sheng Tai Xue Bao ; 31(5): 1753-1762, 2020 May.
Artigo em Chinês | MEDLINE | ID: mdl-32530255

RESUMO

Amino sugars (AS) are one of the important biochemical components in the natural organic matter pool. Clarifying the sources and transformations of AS would facilitate our understan-ding of the microbial regulation of organic matter. As an emerging technology, compound-specific isotope analysis of amino sugars (CSIA-AS) provides more detailed dynamic information of indivi-dual AS in natural environment. Here, we systematically summarized the determination methods of CSIA-AS and gave an overview on innovative applications in the cycling of AS. CSIA-AS can be performed by gas chromatography-isotope ratio mass spectrometry (GC-IRMS) and ion chromatography-isotope ratio mass spectrometry (IC-IRMS). Each method has its own advantages and disadvantages, but reliable results can be achieved after calibration. The mean residence time of AS is relatively low in soil organic matter, and the bacterial-derived muramic acid possesses a higher minera-lization rate than glucosamine, galactosamine, and mannosamine. The source and metabolic transformation of AS are affected by the substrate, which is related to the specific response of microbial community to different carbon and nitrogen sources. The promotion of CSIA-AS technology requires further optimization of method and integration with other approaches such as microbial screening to decipher the source, transformation, fate and regulatory mechanisms of organic matter.


Assuntos
Amino Açúcares , Carbono , Isótopos de Carbono , Isótopos de Nitrogênio , Solo
3.
Sensors (Basel) ; 20(12)2020 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-32545653

RESUMO

The rapid development of urbanization has increased traffic pressure and made the identification of urban functional regions a popular research topic. Some studies have used point of interest (POI) data and smart card data (SCD) to conduct subway station classifications; however, the unity of both the model and the dataset limits the prediction results. This paper not only uses SCD and POI data, but also adds Online to Offline (OTO) e-commerce platform data, an application that provides customers with information about different businesses, like the location, the score, the comments, and so on. In this paper, these data are combined to and used to analyze each subway station, considering the diversity of data, and obtain a passenger flow feature map of different stations, the number of different types of POIs within 800 m, and the situation of surrounding OTO stores. This paper proposes a two-stage framework, to identify the functional region of subway stations. In the passenger flow stage, the SCD feature is extracted and converted to a feature map, and a ResNet model is used to get the output of stage 1. In the built environment stage, the POI and OTO features are extracted, and a deep neural network with stacked autoencoders (SAE-DNN) model is used to get the output of stage 2. Finally, the outputs of the two stages are connected and a SoftMax function is used to make the final identification of functional region. We performed experimental testing, and our experimental results show that the framework exhibits good performance and has a certain reference value in the planning of subway stations and their surroundings, contributing to the construction of smart cities.

4.
Chemosphere ; 255: 126983, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32402867

RESUMO

The instability and rapid consumption of H2O2 limit the application of UV/H2O2 in water treatment. Recently, calcium peroxide (CaO2) has been demonstrated as an effective source of H2O2. However, the performance and mechanism of UV/CaO2 are still unknown. Herein, UV/CaO2 and UV/H2O2 were compared for degradation of aniline. The removal efficiency of aniline by UV/CaO2 was slightly lower than that by UV/H2O2, which could be attributed to the light scavenger by CaO2 suspended particles. HO‧ was identified to participate in aniline degradation in both UV/CaO2 and UV/H2O2, while O2-· was only involved in UV/CaO2. The efficiency of aniline degradation in UV/CaO2 was affected by the released H2O2 in the system. The release and decomposition rate of H2O2 in UV/CaO2 system were influenced by the CaO2 dosage and reaction pH, but slightly related with water matrix. Excessive CaO2 would scavenge aniline degradation through the released H2O2 to react with HO‧. Acidic condition would enhance the concentration of H2O2 in UV/CaO2 and promote the degradation of aniline. Cl- showed slight and almost no effect on aniline degradation in UV/CaO2 and UV/H2O2 systems, respectively, while HCO3- scavenged aniline degradation in UV/CaO2. NO3- inhibited aniline degradation in both UV/CaO2 and UV/H2O2. Compared to UV/H2O2, UV/CaO2 shows the similar efficiency on organics removal but conquers the limitations in UV/H2O2, which is a promising alternative choice in water treatment.

6.
Zootaxa ; 4722(2): zootaxa.4722.2.5, 2020 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-32230633

RESUMO

Morphological and molecular analyses have determined that there is a new species of Tardigrada found in China. Diphascon wuyingensis sp. nov., has smooth cuticle, pharyngeal apophyses, three rod-shaped macroplacoids (increasing in length from first to third, with the second macroplacoid clearly longer than the first) and lacks microplacoids and septulum. The new species has a very small drop-shaped formation and small claws of the Hypsibius type, but no pseudolunules or other cuticular thickenings. Three individual specimens and a group of four specimens were used for DNA isolation and 18S rRNA and COI sequencing; the p-distances to another three Diphascon species used for comparison varied in ranges of 8.8-10.2% (18S rRNA) and 24.2-26.7% (COI).


Assuntos
Tardígrados , Animais , China , Faringe
7.
Eur J Radiol ; 126: 108962, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32244066

RESUMO

PURPOSE: To evaluate the clinical benefits and complications of vesselplasty using the Mesh-Hold™ bone-filling container in the treatment of vertebral osteolytic fractures. METHODS: This was a retrospective study of patients with vertebral osteolytic pathological fractures treated by vesselplasty at Sichuan Cancer Hospital between 09/2014 and 01/2018. VAS1 (Visual analog scale) scores and ODI2 (Oswestry disability index) were recorded routinely 1 day preoperative, at 1 day, 1 month, 3 months, 6 months, and 1 year postoperation, and at the last follow-up. V13 (The of bone cement injection volume) and V24 (vertebral body osteolytic volume) were evaluated, and the R5 (ratio) of bone cement filling was obtained according to the V1/V2. RESULTS: Sixty-three patients were included (105 segments with osteolytic fractures). The amount of bone cement for each vertebra was 2.4-5.2 ml (3.1 ± 0.7 ml). The ratio (R) of bone cement filling was not related to pain relief or functional recovery (all P > 0.05).The VAS scores and ODI at different time points after surgery were decreased compared with before surgery (all P < 0.05). The bone cement leakage rate was 16.2 % (17/105). The follow-up was 4-30 months (mean of 13 ± 6 months). Thirty patients had died by the last follow-up, all from their cancer. CONCLUSIONS: The Mesh-Hold™ bone-filling container in the treatment of vertebral fractures induced by osteolytic metastases could reduce pain, improve function, and reduce the bone cement leakage rate in the process of vesselplasty.

8.
Viral Immunol ; 2020 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-32315577

RESUMO

As a zoonotic disease, ovine contagious pustular dermatitis (Orf) is a serious threat to sheep as well as humans. Orf virus (ORFV) interferon resistance protein (VIR) is the principal virulence protein that encodes a dsRNA-binding protein to inhibit host antiviral response. p53 is one of the key proteins of the host antiviral innate immunity. It not only enhances type I interferon secretion but also induces apoptosis in infected cells, and plays a crucial role in the immune response against various viral infections. However, it remains to be elucidated what role p53 plays in ORFV replication and whether ORFV's own protein VIR regulates p53 expression to promote self-replication. In this study, we showed that p53 has an antiviral effect on ORFV and can inhibit ORFV replication. In addition, ORFV nonstructural protein VIR interacts with p53 and degrades p53, which inhibits p53-mediated positive regulation of downstream antiviral genes. This study provides new insight into the immune evasion mediated by ORFV and identifies VIR as an antagonistic factor for ORFV to evade the antiviral response.

9.
Ecotoxicol Environ Saf ; 197: 110620, 2020 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-32311615

RESUMO

The aim of this study was to evaluate the ecotoxic effect of high concentration cesium (Cs) exposure on plant root growth and its toxicological mechanism. The radicle of broad bean (Vicia faba) was selected as experimental material. The cytotoxic and genotoxic effects of plants exposed to different Cs levels (0.19-1.5 mM) for 48 h were evaluated using scanning electron microscopy (SEM), X-ray fluorescence (XRF) analysis, single-cell gel electrophoresis (SCGE) and random amplified polymorphic DNA (RAPD) assays. The results showed that radicle elongation decreased clearly after 48 h of exposure treatment with different concentrations of Cs solution. The root cell structure was obviously damaged in the Cs treatment groups (0.19-1.5 mM). At a Cs concentration of 1.5 mM, the percentages of viable non-apoptotic cells, viable apoptotic cells, non-viable apoptotic cells, and non-viable cells were 40.09%, 20.67%, 28.73%, and 10.52%, respectively. SCGE showed DNA damage in radicle cells 48 h after Cs exposure. Compared with the control group, the percentage of tail DNA in Cs exposed group (0.38-1.5 mM) increased by 0.56-1.12 times (P < 0.05). RAPD results showed that the genomic stability of V. faba radicles decreased by 4.44%-15.56%. This study confirmed that high concentration Cs exposure had cytotoxicity and genotoxicity effects on plants.

10.
Clin Chim Acta ; 508: 33-42, 2020 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-32348785

RESUMO

Intervertebral disc degeneration (IDD) is one of the main causes of low back pain, which seriously reduces the quality of life of patients and places a heavy economic burden on their families. Cellular senescence is considered to be an important factor leading to IDD, and inflammatory response, oxidative stress, and mitochondrial dysfunction are closely related to intervertebral disc (IVD) senescence. Therefore, inhibition of the inflammatory response and oxidative stress, along with maintaining mitochondrial function, may be useful in treating IDD. The sirtuins are a family of evolutionarily conserved nicotinamide adenine dinucleotide (NAD+)-dependent histone deacetylases, which are the major molecules mediating life extension or delay of aging-related diseases. The sirtuin protein family consist of seven members (SIRT1 - 7), which are mainly involved in various aging-related diseases by regulating inflammation, oxidative stress, and mitochondrial function. Among them, SIRT1, SIRT2, SIRT3, and SIRT6 are closely related to IDD. In addition, some activators of sirtuin proteins, such as resveratrol, melatonin, magnolol, 1,4-dihydropyridine (DHP), SRT1720, and nicotinamide mononucleotide (NMN), have been evaluated in preclinical studies for their effects in preventing IDD. This review described the biological functions of sirtuins and the important roles of SIRT1, SIRT2, SIRT3, and SIRT6 in IDD by regulating oxidative stress, inflammatory response, and mitochondrial function. In addition, we introduce the status of some sirtuin activators in IDD preclinical studies. This review will provide a background for further clarification of the molecular mechanism underlying IDD and the development of potential therapeutic drugs.

11.
Virus Res ; 281: 197907, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32113834

RESUMO

MALAT1, a long non-coding RNA, is highly expressed in cervical cancer cells and plays an important role in the development of cervical cancer. However, the mechanism for the excessive expression of MALAT1 in cervical cancer remains unclear. High-risk HPVs are causative agents of cervical cancer and the IL-6/STAT3 signaling is closely correlated with the development of various cancers including cervical cancer. In this study, the roles of HPV18 E6/E7 and IL-6/STAT3 in the regulation of MALAT1 transcription in cervical cancer cells were investigated. It was found that HPV18 E6/E7 activated the IL-6/STAT3 signaling and, in reciprocal, IL-6/STAT3 strengthened HPV18 E6/E7 expression in HeLa cells. Both HPV18 E6/E7 and IL-6/STAT3 were involved in MALAT1 expression and they worked synergistically in the upregulation of MALAT1 gene. With luciferase reporter assays, a STAT3-binding sequence in the enhancer region of MALAT1 gene was demonstrated to be crucial for the IL-6- or STAT3-induced MALAT1 promoter activation. Taken together, our data suggest that IL-6/STAT3 mediates the HPV18 E6/E7 stimulated upregulation of MALAT1 gene in cervical cancer HeLa cells.

12.
Medicine (Baltimore) ; 99(9): e19247, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32118730

RESUMO

RATIONALE: The capsular warning syndrome (CWS) is a rare and special type of transient ischemic attacks (TIAs) syndrome. The pathophysiology of CWS is very complicate, and intracranial atherosclerotic stenosis (ICAS) is rare cause. Moreover, the effective and standard therapy has not yet been established. PATIENT CONCERNS: A 47-year-old man experienced repeated and exacerbated TIAs of right hemiparesis and dysarthria. Fourteen hours after the first episode of TIAs, he developed more severe right hemiparesis and dysarthria, the National Institute of Health Stroke Scale (NIHSS) score was 12 points, and did not recover in a long time. DIAGNOSIS: The computed tomography (CT) angiography displayed high stenosis in the M1 segment of the left middle cerebral artery. The patient was diagnosed as CWS with ICAS. INTERVENTIONS: Loading dose of clopidogrel and aspirin were started but were ineffective, then we used recombinant tissue plasminogen (r-tPA) for thrombolysis therapy after repeat CT scan that showed small acute infarcts in the right putamen and no bleeding. OUTCOMES: The patient was successfully treated by r-tPA intravenous thrombolysis after loading dose of dual-anti-platelet. He recovered rapidly, and the NIHSS score was 0 point, modified Rankin Scale score was 0 point, and Barthel Index score was 100 points at 3-month follow-up. LESSONS: r-tPA combined with loading dose of dual antiplatelet appears safe and effective in carefully selected CWS patients with ICAS. The collection of similar cases and further randomized controlled trial research would be desirable.


Assuntos
Fibrinolíticos/uso terapêutico , Arteriosclerose Intracraniana/tratamento farmacológico , Ataque Isquêmico Transitório/tratamento farmacológico , Aspirina/administração & dosagem , Aspirina/uso terapêutico , Clopidogrel/administração & dosagem , Clopidogrel/uso terapêutico , Angiografia por Tomografia Computadorizada , Diagnóstico Diferencial , Fibrinolíticos/administração & dosagem , Humanos , Arteriosclerose Intracraniana/complicações , Arteriosclerose Intracraniana/diagnóstico por imagem , Ataque Isquêmico Transitório/complicações , Ataque Isquêmico Transitório/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Artéria Cerebral Média/diagnóstico por imagem , Síndrome , Ativador de Plasminogênio Tecidual/administração & dosagem , Ativador de Plasminogênio Tecidual/uso terapêutico , Tomografia Computadorizada por Raios X
13.
J Biomed Mater Res A ; 108(6): 1351-1368, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32090432

RESUMO

The prosthetic mesh, which is widely used in tension-free hernioplasty, often result in avascular stiff fibrotic scar or mesh shrinkage, causing chronic pain and infection. Here, we developed an autologous bionic tissue (ABT), which was composed of autologous bone marrow-derived mesenchymal stem cells (MSCs), poly (lactic-co-glycolic acid) (PLGA) porous scaffolds, and extracellular matrix (ECM) produced by MSCs for inguinal hernioplasty. In ABT, MSCs produced a variety of ECM composites, such as structural proteins (insoluble collagen, elastin) that provided mechanical properties, macromolecules (hyaluronic acid, glycosaminoglycan) as water and cytokines reservoir, and cell-engaging proteins (fibronectin, laminin). The above ECM composites reached the highest level in 21 days. ECM degradation related cytokines (MMP-9 and its inhibitor TIMP-1) reached the highest level on the 14th day. ECM increased the mechanical properties, elasticity, and flexibility of PLGA. Compared with the PLGA, ABT greatly inhibited inflammatory factors and promoted anti-inflammatory factors (p < 0.05), and gradually reduced the M1/M2 ratio in vivo (p < 0.05). After implantation, the thickness of tissue regeneration (p < 0.05), the number of capillaries or mature vessels (p < 0.05), the mechanical properties of ABT (p < 0.05) were greater than PLGA. MSCs and ECM could reduce the inflammation caused by PLGA, and prevent PLGA from earlier degradation and facilitate host cellular infiltration, thus ABT could greatly promote tissue regeneration in hernia repairs.

14.
Bioresour Technol ; 302: 122881, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32014732

RESUMO

In this study, a reliable approach using ammonia nitrogen was proposed to increase lactate production during semi-continuous food waste (FW) fermentation under mesophilic conditions. Both free ammonia nitrogen (FAN) and ammonium ion (NH4+-N) were present in mesophilic reactors, with a wide FAN/NH4+-N ratio variation due to the intermittent pH control. The investigation of responsible mechanisms revealed that the increased production yield of LA was associated with the acceleration of solubilization, hydrolysis, glycolysis and acidification. The presence of FAN and NH4+-N in proper concentrations increased lactate production by 2.4 folds and recovered lactate production to 24.5 g COD/L from low rate control reactor (9.6 g COD/L) under mesophilic conditions. Furthermore, the microorganisms responsible for LA accumulation (Bavariicoccus, Enterococcus, Bifidobacterium and Corynebacterium) were selectively enriched, and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways associated with carbohydrate transport and LA production were enhanced in nitrogen fed reactors.


Assuntos
Amônia , Eliminação de Resíduos , Reatores Biológicos , Fermentação , Alimentos , Ácido Láctico
15.
Funct Plant Biol ; 47(4): 318-326, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32054564

RESUMO

Organic acids secreted from the roots of plants play important roles in nutrient acquisition and metal detoxification; however, the precise underlying mechanisms of these processes remain poorly understood. In the present study we examined the content of organic acids exuded from roots and the effects of these organic acids on the activation of slowly available potassium (K) at different K levels, including normal K supply and K-deficient conditions. In addition, the study system also comprised a high-K tobacco variety (ND202) and two common ones (K326 and NC89). Our results showed that high-K varieties exhibited significantly higher contents of organic acids in its root exudates and available K in both rhizosphere and non-rhizosphere soils than the other varieties. This research also suggested that a cyclic process in which soil was acidified after being complexed by organic acids was involved in the release of slowly available K, and that this process primarily depended on the soil pH at high organic acids concentrations, but the complexation of organic ligands became dominant at low concentrations. In conclusion, tobacco roots secrete organic acids to increase available K content and improve the utilisation rate of soil K. High-K varieties probably enhance slowly available K activation by secreting relatively high amounts of organic acids, thus leading to more available K in soil for absorption by plants.

16.
ACS Sens ; 5(2): 327-337, 2020 02 28.
Artigo em Inglês | MEDLINE | ID: mdl-31989811

RESUMO

The monitoring of biopharmaceutical critical quality attributes in-process, at both the process development and manufacturing stages, is necessary for the implementation of process analytical technology and quality-by-design principles. Among these attributes, it is important to monitor and control protein aggregation during the manufacturing of biological therapeutics to prevent adverse immunogenic responses and minimize negative impacts on drug deliverability. In this work, we explore hydrogel-encapsulated, label-free fluorescent nanosensors for the characterization of protein aggregation. A mathematical model is used to describe the diffusion and binding of a series of stressed pharmaceutical samples to such sensors, describing their dynamic response. We use mathematical modeling to map the influence of hydrogel properties on the separation performance, given the composition of UV-stressed IgG1 samples. Using this modified model, the compositions of light-stressed IgG1 samples were fit to experimental data and correlated with size-exclusion chromatography data. The results demonstrate the ability to detect the presence of high-molecular-weight protein species at a concentration as low as 1%. This work represents a significant step toward the development and deployment of rapid process analytical technologies for biopharmaceutical characterization.

17.
Bioresour Technol ; 300: 122709, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31901771

RESUMO

Bio-valorization of organic waste streams, such as food waste and waste activated sludge, to lactic acid (LA) has recently drawn much attention. It offers an opportunity for resource recovery, alleviates environmental issues and potentially turns a profit. In this study, both stable and high LA yield (0.72 ± 0.15 g/g total chemical oxygen demand) and productivity rate (0.53 g/L•h) were obtained through repeated batch fermentation. Moreover, stable solubilization and increase in the critical hydrolase activities were achieved. Depletions of ammonia and phosphorus were correlated with the LA production. The relative abundance of the key LA bacteria genera (i.e., Alkaliphilus, Dysgonomonas, Enterococcus and Bifidobacterium) stabilized in the repeated batch reactor at a higher level (44.5 ± 2.53%) in comparison with the batch reactor (26.2 ± 4.74%). This work show a practical way for the sustainable valorization of organic wastes to LA by applying the repeated batch mode during biological treatment.


Assuntos
Eliminação de Resíduos , Esgotos , Reatores Biológicos , Fermentação , Alimentos , Ácido Láctico
18.
J Hazard Mater ; 386: 121437, 2020 03 15.
Artigo em Inglês | MEDLINE | ID: mdl-31899027

RESUMO

Uranium (U) is a nonessential element that is readily adsorbed and retained in plant roots, causing root damage plants, rather than being translocated to other parts of the plant. The phytotoxicity mechanism of U is poorly understood. In this study, Vicia faba, a model plant for toxicological research, was selected as experimental material to investigate the phytotoxicity mechanism of U. In this study, the effects of U on the growth and development, methonome, transcriptome and mineral nutrient metabolism of V. faba were studied under different U treatments (0-25 µM) by integrating metabolomics, transcriptomic, and mineral nutrient metabolism analysis techniques. The results showed that U accumulation in roots and aboveground parts reached 164.34-927.90 µg/pot, and 0.028-0.119 µg/pot, respectively. U was mainly accumulated in the cell wall of roots, which damaged the root microstructure and inhibited root growth and development. In terms of mineral nutrient metabolism, U treatment (0-25 µM) led to changes in mineral metabolic profiles of seedlings. In total, 612 different metabolites were identified in nontargeted metabolomics, including 309 significantly upregulated metabolites and 303 significantly downregulated metabolites. Using RNA-seq, 4974 differentially expressed genes (DEGs) were identified under the high-concentration U treatment (25 µM), including 1654 genes significantly upregulated genes and 3320 genes significantly downregulated genes. Metabolic pathway analysis showed that a high concentration of U led to an imbalance of mineral nutrient metabolism in plants and changes in the metabolism and transcriptome pathway of plants, including alterations in the function of plasmodesmata and auxin signal transduction pathway. The latter finding may potentially explain the toxic effect of U on plant roots.

19.
Chem Commun (Camb) ; 56(10): 1521-1524, 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-31922153

RESUMO

Tremendous advancements in proteolysis targeting chimera (PROTAC) technology have been made in recent years. However, whether a covalent inhibitor-based PROTAC can be developed remains controversial. Here, we successfully developed chimeric degraders based on covalent inhibitors to degrade BTK and BLK kinases, demonstrating that covalent inhibitor-based PROTACs are viable and useful tools.


Assuntos
Tirosina Quinase da Agamaglobulinemia/metabolismo , Técnicas Biossensoriais/métodos , Inibidores de Proteínas Quinases/química , Quinases da Família src/metabolismo , Tirosina Quinase da Agamaglobulinemia/antagonistas & inibidores , Humanos , Células K562 , Proteólise , Ubiquitina-Proteína Ligases/metabolismo , Quinases da Família src/antagonistas & inibidores
20.
Acta Neurol Belg ; 120(1): 37-42, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29623601

RESUMO

The objective of this study is to investigate effects of minimally invasive approaches on outcome of chronic subdural hematoma (CSDH) by novel YL-1 puncture needle and burr-hole methods. A retrospective analysis was performed in 158 hospitalized CSDH patients from January, 2013 to December, 2017 in Kunshan Hospital of Traditional Chinese Medicine. Patients' gender, age, history of trauma, volume of hematoma, hematoma location, application of urokinase, surgical approach, the operation time, hospitalized time, and CT scans 3 months after discharge were recorded. Prognostic indicators including symptom relief and post-hospital neuro-imaging findings were extracted to evaluate surgical efficacy. Statistical methods were conducted to evaluate surgical efficacy. Both YL-1 puncture needle and burr-hole surgeries had a satisfying follow-up (93.67%). There was non-significant group difference in follow-up results (p > 0.05). While YL-1 needle group needs less operation time ((p < 0.001) and hospitalized time (p < 0.001), gender (p = 0.144), age (p = 0.394), history of head trauma (p = 0.445), volume of hematoma (p = 0.068), hematoma location (p = 0.281), and application of urokinase (p = 0.545) were shown non-significantly associated with these two minimally invasive approaches. Volume of hematoma was significantly associated with follow-up outcomes (p = 0.016). Novel YL-1 puncture needle and classic burr-hole craniotomy are both proved to be safe and effective minimally invasive surgeries, which can provide an early intervention and minimally invasive strategy for neurosurgeons.

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