Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 7 de 7
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Eur Rev Med Pharmacol Sci ; 24(1): 267-273, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31957840

RESUMO

OBJECTIVE: The aim of this study was to investigate the effects of microRNA-5195-3p (miR-5195-3p) on the proliferation of glioma cells and to explore its related mechanisms. PATIENTS AND METHODS: The expression level of miR-5195-3p in glioma tissues and cell lines was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Online prediction websites (TargetScan and miRanda) were used to screen the potential targets of miR-5195-3p. Luciferase reporter gene assay and Western blot were performed to confirm the targets of miR-5195-3p. Furthermore, the effects of miR-5195-3p on cell proliferation were detected by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide), colony formation assay, and flow cytometry, respectively. RESULTS: MiR-5195-3p was lowly expressed in both glioma tissues and cells. Baculoviral IAP repeat-containing 2 (BIRC2) was identified as a direct target of miR-5195-3p. Over-expression of miR-5195-3p in glioma cells significantly decreased the protein expression of BIRC2. Besides, the proliferative capacity and colony formation ability were significantly inhibited after transfection of miR-5195-3p in vitro. In addition, flow cytometry indicated that an evident G1 phase arrest occurred in miR-5195-3p over-expressed group. CONCLUSIONS: In this work, we emphasized the suppressor function of miR-5195-3p in the proliferation of glioma cells. Furthermore, our findings provided an experimental basis for the research and treatment of glioma.

2.
Zhonghua Liu Xing Bing Xue Za Zhi ; 40(4): 422-426, 2019 Apr 10.
Artigo em Chinês | MEDLINE | ID: mdl-31006202

RESUMO

Objective: To characterize the relations between the practice of parenting and associated factors on children (0-5 years old) in urban areas of China, in order to provide evidence for promoting the early development of children and to provide positive guidance and service programs on parenting. Methods: A total of 4 515 parents from 15 cities (14 provinces) were surveyed with a self-administered questionnaire. Parenting and Family Adjustment Scales (PAFAS) was used, including parameters as: consistency and coercive parenting, positive encouragement, parent-child relationship and parental emotion adjustment, family relationship and parental teamwork aspects, etc. Both single factor analysis and multiple linear regression were used to examine the associations between parenting practice, individual, parental and family factors. Results: The mean score of PAFAS was 21.00 (15.00-28.00), associated with factors as children's age, only-child family, premature delivery, father's education level, confidence on parenting, problems regarding the parental mood, annual family income, family structure and behavior on seeking professional help, etc. Results showed that there were big differences on the practice of parenting in China and influenced by variety of factors. Conclusions: The general situation of parenting was well, in the urban areas of China. The practice of parenting was associated with a series of individual, parental and family factors. Programs on improving the parenting skills and promoting the early development of children, should be highlighted.


Assuntos
Educação Infantil , Relações Pais-Filho , Poder Familiar , Criança , Pré-Escolar , China , Humanos , Lactente , Recém-Nascido , Masculino , Pais , População Urbana
3.
Braz J Med Biol Res ; 51(3): 1-7, 2018 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-29513878

RESUMO

Breast cancer is the most common cause of cancer among women in most countries (WHO). Ovarian hormone disorder is thought to be associated with breast tumorigenesis. The present study investigated the effects of estrogen and progesterone administration on cell proliferation and underlying mechanisms in breast cancer MCF-7 cells. It was found that a single administration of estradiol (E2) or progesterone increased MCF-7 cell viability in a dose-dependent manner and promoted cell cycle progression by increasing the percentage of cells in the G2/M phase. A combination of E2 and progesterone led to a stronger effect than single treatment. Moreover, cyclin G1 was up-regulated by E2 and/or progesterone in MCF-7 cells. After knockdown of cyclin G1 in MCF-7 cells using a specific shRNA, estradiol- and progesterone-mediated cell viability and clonogenic ability were significantly limited. Additionally, estradiol- and progesterone-promoted cell accumulation in the G2/M phase was reversed after knockdown of cyclin G1. These data indicated that estrogen and progesterone promoted breast cancer cell proliferation by inducing the expression of cyclin G1. Our data indicated that novel therapeutics against cyclin G1 are promising for the treatment of estrogen- and progesterone-mediated breast cancer progression.


Assuntos
Neoplasias da Mama/patologia , Proliferação de Células/efeitos dos fármacos , Ciclina G1/metabolismo , Estrogênios/farmacologia , Progesterona/farmacologia , Western Blotting , Neoplasias da Mama/metabolismo , Sobrevivência Celular , Feminino , Humanos , Células MCF-7/efeitos dos fármacos , Reação em Cadeia da Polimerase em Tempo Real
4.
Bull Exp Biol Med ; 160(5): 715-7, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27021080

RESUMO

We developed wound coating based on natural biopolymer chitosan with additional components (ceruloplasmin, L-asparaginic acid, and glycerol). Experiments on albino male rats demonstrated its regeneratory, antioxidant, and antibacterial effects on wounds involving all layers of the skin. Due to chemical composition and buffer component, the biodegraded wound coating optimizes all phases of the wound process, accelerates by 22-28% the reparative regeneration, and leads to anatomic and functional restoration of injured sites. High absorption capacity recommends its use in the treatment of wounds with profuse exudation.


Assuntos
Antibacterianos/uso terapêutico , Antioxidantes/uso terapêutico , Quitosana/uso terapêutico , Regeneração Tecidual Guiada/métodos , Pele/lesões , Cicatrização/efeitos dos fármacos , Animais , Ceruloplasmina/uso terapêutico , Glicerol/uso terapêutico , Masculino , Ratos
5.
Ross Fiziol Zh Im I M Sechenova ; 102(3): 351-61, 2016 Mar.
Artigo em Russo | MEDLINE | ID: mdl-30188669

RESUMO

Instrumental and laboratory monitoring of bone tissue adaptive remodeling processes after total hip replacement conducted for primary osteoarthrosis patients (32 males and 48 females) (coxarthrosis stage III, functional insufficiency stages II and III) with long-lasting joint damage, significant pain syndrome and osteodeficit, indicated osteodeficit intensification compared to the baseline in the implant surrounding areas. These patients showed an increasing cytokine imbalance in the blood serum postsurgically with the primary activation of proinflammatory (osteoresorbing) element and secondary activation of anti-inflammatory element. Structural and functional characteristics of bone tissue in the area adjacent to the implant and cytokine balance indexes in the blood serum did not return to the baseline even 12-15 months postsurgically.


Assuntos
Artroplastia de Quadril/métodos , Remodelação Óssea , Osteoartrite/cirurgia , Complicações Pós-Operatórias/sangue , Adulto , Idoso , Artroplastia de Quadril/efeitos adversos , Interface Osso-Implante/patologia , Citocinas/sangue , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias/patologia
6.
Folia Biol (Praha) ; 57(3): 87-95, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21888831

RESUMO

It has been shown that drug resistance is extremely common in hepatocellular carcinoma (HCC) and is one of the major problems in HCC chemotherapy. However, the detailed mechanisms remain largely unknown. We have previously shown that endoplasmic reticulum (ER) stress is involved in the tumorigenesis of HCC. Here, we demonstrated that the unfolded protein response (UPR) inhibits cisplatin-induced HCC cell apoptosis. In HCC cells, cisplatin treatment triggers the UPR, which subsequently inhibits cisplatin-induced apoptosis. Importantly, mild ER stress precondition suppresses the sensitivity of HCC cells to cisplatin-induced apoptosis through autophagy regulation. Furthermore, heat-shock protein 27 (Hsp27) is involved in the cytoprotective role of the UPR in cisplatin-induced apoptosis. We also demonstrated that Hsp27 inhibits cisplatin- induced HCC cell death through autophagy activation. Taken together, our results indicate that the UPR inhibits cisplatin-induced apoptosis in HCC cells, at least in part, by Hsp27-mediated autophagy activation.


Assuntos
Apoptose/efeitos dos fármacos , Autofagia/efeitos dos fármacos , Autofagia/fisiologia , Carcinoma Hepatocelular/patologia , Cisplatino/farmacologia , Resposta a Proteínas não Dobradas/fisiologia , Antineoplásicos/farmacologia , Apoptose/fisiologia , Linhagem Celular Tumoral , Cisplatino/metabolismo , Ditiotreitol/farmacologia , Retículo Endoplasmático/efeitos dos fármacos , Retículo Endoplasmático/fisiologia , Proteínas de Choque Térmico HSP27/genética , Proteínas de Choque Térmico HSP27/metabolismo , Resposta ao Choque Térmico , Humanos , Neoplasias Hepáticas/patologia , Tunicamicina/farmacologia
7.
Folia Biol (Praha) ; 56(2): 37-46, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20492754

RESUMO

The potential pro-survival role of phosphatidylinositol 3-kinase (PI3K)/Akt during endoplasmic reticulum stress has been well-characterized. However, the detailed mechanisms remain largely unknown. Here, we showed that PI3K/Akt inhibition promoted endoplasmic reticulum stress-induced apoptosis in a glucose-regulated protein 78 (GRP78)-dependent manner. During endoplasmic reticulum stress, high levels of Akt phosphorylation were sustained for at least 18 h in HEK293 cells. Importantly, PI3K/Akt enhanced GRP78 accumulation through increasing its stability following endoplasmic reticulum stress. Furthermore, Akt1, but not Akt2 or Akt3, was involved in GRP78 stability regulation. These results suggest that PI3K/Akt inhibits endoplasmic reticulum stress-induced apoptosis in HEK293 cells, at least in part, by promoting GRP78 protein stability.


Assuntos
Apoptose/fisiologia , Retículo Endoplasmático/metabolismo , Proteínas de Choque Térmico/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Estresse Fisiológico , Animais , Apoptose/efeitos dos fármacos , Linhagem Celular , Ditiotreitol/farmacologia , Retículo Endoplasmático/efeitos dos fármacos , Inibidores Enzimáticos/farmacologia , Proteínas de Choque Térmico/genética , Humanos , Camundongos , Células NIH 3T3 , Fosfatidilinositol 3-Quinases/genética , Proteínas Proto-Oncogênicas c-akt/genética , Tapsigargina/farmacologia , Fator de Transcrição CHOP/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA