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1.
Org Lett ; 2020 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-32186888

RESUMO

A visible-light-driven photoredox-catalyzed multicomponent reaction of 2-vinylanilines, sulfonyl chlorides, and sulfur ylides is described. This protocol features redox-neutral mild conditions, a broad substrate scope, and good functional group tolerance, providing access to various sulfonated 2,3-disubstituted indolines. The product can be transformed to a diverse range of functionalized indoles by a selective aromatization/nucleophilic substitution process. Mechanistic investigations suggest that both sulfonyl chlorides and sulfur ylides serve as radical sources, and the reaction proceeds through a sequential radical addition/addition/thermal SN2-substitution process.

2.
J Pharm Biomed Anal ; 180: 113045, 2020 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-31887668

RESUMO

Nintedanib is a promising tyrosine kinase inhibitor for clinically treating idiopathic pulmonary fibrosis (IPF). Some clinical cases reported that nintedanib treatment can cause hepatotoxicity and myocardial toxicity. U. S. FDA warns the potential drug-drug interaction when it is co-administrated with other drugs. In order to understand the potential toxicity of nintedanib and avoid drug-drug interaction, the metabolism of nintedanib was systematically investigated in human liver microsomes and mice using metabolomics approach, and the toxicity of metabolites was predicted by ADMET lab. Nineteen metabolites were detected in vivo and in vitro metabolism, and 8 of them were undescribed. Calculated partition coefficients (Clog P) were used to distinguish the isomers of nintedanib metabolites in this study. The major metabolic pathways of nintedanib majorly included hydroxylation, demethylation, glucuronidation, and acetylation reactions. The ADMET prediction indicated that nintedanib was a substrate of the cytochrome P450 3A4 (CYP3A4) and P-glycoprotein (P-gp). And nintedanib and most of its metabolites might possess potential hepatotoxicity and cardiotoxicity. This study provided a global view of nintedanib metabolism, which could be used to understand the mechanism of adverse effects related to nintedanib and its potential drug-drug interaction.

3.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 27(4): 1118-1122, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31418366

RESUMO

OBJECTIVE: To explore the significance of lymphocyte to monocyte ratio (LMR) in the disease progress of primary gastrointestinal diffuse large B-cell lymphoma (PGI-DLBCL). METHODS: The clinical data of 43 patients diagnosed as PGI-DLBCL in our hospital from January 2011 to December 2015 were collected, and the disease progress was followed up. RESULTS: According to the ROC curve, the threshold value of LMR for 2 years PFS (%) of PGI-DLBCL patients was 2.6. Unvariate analysis showed that LMR (P<0.05), large enclosed mass lesion (P<0.01) and IPI (P<0.05) were prognostic factors affecting PFS, the COX regression model multivariate analysis showed that LMR<2.6 [ (risk ratio (RR)=3.083, 95%CI 1.828-8.313, P<0.01], and large enclosed mass lesions (RR=2.718, 95%CI 1.339-6.424, P<0.05) were the independent adverse prognostic factor for two years PFS. CONCLUSION: Both LMR<2.6 and large enclosed mass lesions relate with the progress of PGI-DLBCL.


Assuntos
Linfoma Difuso de Grandes Células B , Monócitos , Humanos , Contagem de Leucócitos , Linfócitos , Prognóstico , Estudos Retrospectivos
4.
J Microbiol Immunol Infect ; 52(5): 710-719, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31427111

RESUMO

BACKGROUND: The regimen containing tenofovir disoproxil fumarate (TDF)+lamivudine or emtricitabine + efavirenz remains the recommended first-line antiretroviral therapy (ART) by the WHO. Limited studies, however, have been conducted on the incidence of renal impairment among Chinese patients with long-term exposure to TDF-containing ART regimens. METHODS: We retrospectively analyzed 269 eligible patients who had no comorbidities and received TDF-containing ART from July 2014 to April 2015. TDF-related renal impairment was defined as a decrease of eGFR by >25% from baseline or eGFR <90 ml/min/1.73 m2. Decreased renal function was defined as a decrease of eGFR by > 10 mL/min/1.73 m2 from baseline. RESULTS: 97.0% of study patients were male (median age 29, eGFR 124.0 ml/min/1.73 m2). After 168-week of ART, renal impairment occurred in 7 patients (2.7%). The incidence of decreased renal function was significantly higher at Week 168 compared with that observed at Week 12 (24.8% vs 3.7%, p < 0.001). In generalized estimating equation analysis, patients receiving ART for 144-week (aOR4.1, 95%CI 2.0-8.4) and 168-week (aOR8.4, 95%CI 4.2-16.4) were more likely to develop decreased renal function compared with those receiving ART for 12-week, so were the patients with a weight <58 kg (aOR2.3, 95%CI 1.2-4.3) and 58-66 kg (aOR2.0, 95%CI 1.0-3.8) compared to those with a weight ≥67 kg. At 168-week, 41.0% of 100 patients examined had elevated urine ß2-microglobulin levels, which were negatively correlated with eGFR (r = -0.22, p = 0.02). CONCLUSIONS: TDF-related renal impairment remained rare in HIV-positive Chinese patients with a median age of 29 years who had no comorbidities. A lower weight and duration of ART were associated with decreased renal function.


Assuntos
Fármacos Anti-HIV/efeitos adversos , Infecções por HIV/tratamento farmacológico , Insuficiência Renal/induzido quimicamente , Tenofovir/efeitos adversos , Adulto , Grupo com Ancestrais do Continente Asiático , Feminino , Taxa de Filtração Glomerular/efeitos dos fármacos , Infecções por HIV/complicações , Hepatite B Crônica/etiologia , Humanos , Rim/efeitos dos fármacos , Túbulos Renais Proximais/efeitos dos fármacos , Masculino , Pessoa de Meia-Idade , Proteinúria , Insuficiência Renal/etiologia , Estudos Retrospectivos , Adulto Jovem
5.
Trials ; 20(1): 343, 2019 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-31182140

RESUMO

BACKGROUND: Spleen qi deficiency (SQD), a syndrome based on traditional Chinese medicine (TCM) theory, is common in patients after radical gastrectomy. SQD manifests with chronic gastrointestinal disorders and systemic symptoms and is challenging to manage. Hou Gu Mi Xi (HGMX) is a dietary TCM formula for SQD. This study aims to evaluate the efficacy and safety of HGMX in patients with SQD who have undergone radical gastrectomy for gastric cancer. METHODS AND DESIGN: This study is a multicenter, randomized, double-blind, placebo-controlled trial. One hundred thirty patients with SQD who have undergone radical gastrectomy for gastric cancer will be assigned to receive either HGMX or placebo for 2 years. The main outcome will be changes in SQD symptoms assessed by the Spleen Qi Deficiency Symptoms Grading and Quantifying Scale. The secondary outcomes will be changes in quality of life assessed by the Short Form 36 scale, performance status as assessed by the Eastern Cooperative Oncology Group Performance Status scale, body weight, and body mass index. Progression-free survival will also be assessed as a secondary outcome. Adverse events (AEs), severe AEs, and study withdrawal due to AEs will be recorded to evaluate the safety of HGMX. DISCUSSION: The results of this trial will provide initial evidence for the use of HGMX as an alternative and complementary intervention to manage chronic postoperative complications in patients who have undergone radical gastrectomy for gastric cancer. TRIAL REGISTRATION: ClinicalTrials.gov, NCT03025152 . Registered on 17 January 2017.


Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Gastrectomia/efeitos adversos , Medicina Tradicional Chinesa , Complicações Pós-Operatórias/tratamento farmacológico , Qi , Neoplasias Gástricas/cirurgia , Adulto , Idoso , Método Duplo-Cego , Medicamentos de Ervas Chinesas/efeitos adversos , Humanos , Pessoa de Meia-Idade , Estudos Multicêntricos como Assunto , Ensaios Clínicos Controlados Aleatórios como Assunto
6.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 27(3): 976-982, 2019 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-31204964

RESUMO

OBJECTIVE: To analyze the incidence of hemorrhagic cystitis (HC) after allogeneic hematopoietic stem cell transplantation and the factors affecting HC, so as to provide clinical evidence for further treatment of HC. METHODS: The HC of 113 patients after allogeneic hematopoietic stem cell transplantation in Affiliated Hospital of Xuzhou Medical University between the years 2014-2016 was analyzed respectively. All cases of HC were divided into HC group and non-HC(control) group. The follow-up time: from preeonditionig day to 180 d after transplantation. The 10 clinical parameters were selected for univariate analysis with COX regression analysis: sex, age (<25 years and 25 years), primary disease, conditioning regimen with anti-thymoglobulin(ATG), sex-mismatch in recipients, haploidential HSCT, cytomegalovirus (CMV) viremia, EB viremia, graft-versus-host disease (GVHD), and primary disease relapse, the factors significant at the 0.1 level in univariate analysis should be further evaluated by multivariate analysis using a COX regression analysis. The difference was significant at P<0.05 in multivariate analysis. RESULTS: The HC occured in 31 of 113 patients (27.4%), with 5 cases of grade I (5.5%), 19 of grade II (16.8%), 5 of grade III (4.4%), and 2 of grade IV (1.8%). The median time of HC onset was 37 days (26-70 d) after transplantation. The median duration of HC was 14 days (5-55d). Univariate analysis showed that conditioning with anti-thymoglobulin (ATG) (RR=6.170, 95%CI: 1.875-20.306, P<0.01), CMV viremia (RR=7.633, 95%CI:2.318-25.133) (P<0.01), haploidentical HSCT (RR=0.307, 95%CI:0.137-0.686, P<0.01), GVHD (RR=1.891, 95%CI:0.918-3.898, P>0.05) were the risk factors for recovery from HC. The multivatiate analysis of above-mentioned risk factors with statistical significance showed that only CMV viremia (RR=4.770, 95%CI: 1.394-16.326, P<0.05) was the indentified risk factor affecting the recovery from HC. CONCLUSION: Monitoring CMV viremia and antivirotic treatment are effective measurs to prevent the occurrence of HC and promote the recovery from HC.


Assuntos
Cistite , Doença Enxerto-Hospedeiro , Transplante de Células-Tronco Hematopoéticas , Humanos , Análise Multivariada , Estudos Retrospectivos , Fatores de Risco
7.
Angew Chem Int Ed Engl ; 58(2): 378-380, 2019 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-30480861

RESUMO

Neutral eosin Y-derived photoexcited states have been found to serve as photoacids and direct hydrogen atom transfer (HAT) catalysts in the activation of glycals and C-H bonds, respectively. These studies pave the way for further use of eosin Y in photochemical synthesis.

8.
Chemistry ; 24(63): 16895-16901, 2018 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-30126062

RESUMO

A new synergistic multicatalytic activation mode of eosin Y has been discovered by exploiting the redox potential of its ground state and excited state. This catalytic strategy proves to be an enabling tool for visible-light-driven sequential benzylic C-H amination and oxidation of o-benzyl-N-methoxyl-benzamides when using Selectfluor as a hydrogen atom transfer (HAT) reagent and O2 as oxidant. Efficient synthesis of a range of diversely functionalized 3-hydroxyisoindolinones can thus be achieved with good yields and selectivity at mild reaction conditions. Preliminary mechanistic studies and DFT calculations suggest that eosin Y works as a redox catalyst and photosensitizer.

9.
Org Lett ; 19(19): 5208-5211, 2017 10 06.
Artigo em Inglês | MEDLINE | ID: mdl-28898097

RESUMO

A ligand-free, palladium-catalyzed aminoarylation reaction of the unactivated alkenes in ß,γ-unsaturated hydrazones is described. This protocol enables efficient and simultaneous formation of C(sp3)-N and C(sp3)-C(sp2) bonds under mild conditions, providing a practical and general approach to various diversely substituted dihydropyrazoles in generally good yields, without the use of any stoichiometric external oxidant.

10.
J Pharm Biomed Anal ; 145: 725-733, 2017 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-28806569

RESUMO

Dehydrodiisoeugenol (DDIE), a representative and major benzofuran-type neolignan in Myristica fragrans Houtt., shows anti-inflammatory and anti-bacterial actions. In order to better understand its pharmacological properties, xenobiotic metabolomics was used to determine the metabolic map of DDIE and its influence on endogenous metabolites. Total thirteen metabolites of DDIE were identified through in vivo and in vitro metabolism, and seven of them were reported for the first time in the present study. The identity of DDIE metabolites was achieved by comparison of the MS/MS fragmentation pattern with DDIE using ultra-performance chromatography electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI- QTOFMS). Demethylation and ring-opening reaction were the major metabolic pathways for in vivo metabolism of DDIE. Recombinant cytochrome P450s (CYPs) screening revealed that CYP1A1 is a primary enzyme contributing to the formation of metabolites D1-D4. More importantly, the levels of two endogenous metabolites 2,8-dihydroxyquinoline and its glucuronide were significantly elevated in mouse urine after DDIE exposure, which explains in part its modulatory effects on gut microbiota. Taken together, these data contribute to the understanding of the disposition and pharmacological activities of DDIE in vivo.


Assuntos
Metabolômica , Animais , Cromatografia Líquida de Alta Pressão , Eugenol/análogos & derivados , Camundongos , Espectrometria de Massas por Ionização por Electrospray , Espectrometria de Massas em Tandem
11.
Org Lett ; 19(13): 3620-3623, 2017 07 07.
Artigo em Inglês | MEDLINE | ID: mdl-28640626

RESUMO

A novel photocatalytic hydrazonyl radical-mediated radical cyclization/allylation cascade reaction of ß,γ-unsaturated hydrazones is developed using allyl sulfones and Morita-Baylis-Hillman adduct as allyl sources, which provides an efficient and practical access to various diversely functionalized dihydropyrazoles and tetrahydropyridazines. The reaction is enabled by controllable generation of hydrazonyl radicals via an oxidative deprotonation electron transfer strategy and selective trapping of the resultant C-centered radicals under visible light irradiation.

12.
Chemistry ; 22(40): 14141-6, 2016 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-27258972

RESUMO

A cooperative TEMPO and photoredox catalytic strategy was applied for the first time to the direct conversion of N-H and O-H bonds into N- and O-centred radicals, enabling a general and selective oxidative radical oxyamination and dioxygenation of various ß,γ-unsaturated hydrazones and oximes. In the reaction, O2 was employed not only as a terminal oxidant but also as the oxygen source. This protocol provided efficient access to the synthesis of various synthetically and biologically important pyrazoline, pyridazine and isoxazoline derivatives under metal-free and mild reaction conditions. Mechanistic studies revealed that the cooperative organophotocatalytic system functions through two single-electron-transfer (SET) processes.

13.
Plast Reconstr Surg ; 137(3): 977-9, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26910682

RESUMO

The purpose of this study was to search for an enhanced blood supply in the distal edge of the skin at the mastoid area in total auricular reconstruction. The authors modified the Nagata method by reserving a subcutaneous fascia pedicle (diameter, 3 to 5 mm) at the intersection of the point 11 to 13 mm from the residual ear tragus (or tragus projection) and 8 to 10 mm from the lowest point of the residual ear lobe. Compared with the traditional Nagata method group, the modified Nagata method group that retained the fascia pedicle of the mastoid skin flap had higher rates of excellent and good flaps (p < 0.05). Auricular reconstruction with the modified Nagata method, retaining the fascia pedicle of the mastoid skin flap, had a reduced incidence of skin flap necrosis.


Assuntos
Microtia Congênita/cirurgia , Pavilhão Auricular/cirurgia , Procedimentos Cirúrgicos Reconstrutivos/métodos , Retalhos Cirúrgicos/transplante , Adolescente , Grupo com Ancestrais do Continente Asiático/estatística & dados numéricos , Criança , Estudos de Coortes , Microtia Congênita/diagnóstico , Microtia Congênita/etnologia , Estética , Fáscia/transplante , Feminino , Seguimentos , Humanos , Masculino , Processo Mastoide/cirurgia , Duração da Cirurgia , Estudos Retrospectivos , Transplante de Pele , Retalhos Cirúrgicos/irrigação sanguínea , Taiwan , Resultado do Tratamento , Cicatrização/fisiologia
14.
Nanoscale ; 7(46): 19484-92, 2015 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-26531170

RESUMO

In this article, a fluorescence-CT dual-mode nanoprobe is successfully synthesized by making use of distearoylphosphatidylethanolamine-poly(ethylene glycol)-folate (DSPE-PEG2000-FA) and other amphiphilic molecules to coat silver sulfide (Ag2S) quantum dots (QDs) and iodinated oil simultaneously. In vitro experiments show that the fluorescence wavelength of the nanoprobe is 1170 nm in the near infrared-II region. Its size is 139.6 nm, it has good dispersibility, and it has low cellular toxicity at concentrations up to 25 µg mL(-1) Ag. In vivo experiments revealed that the probe has a rather long circulation time (blood half-life of 5.7 hours), and the tissue histopathological tests show that it is not obviously harmful to major organs' normal function. Biochemical analysis (glutamic pyruvic transaminase and glutamic oxaloacetic transaminase levels) and blood analysis (white blood cell, red blood cell, hemoglobin and blood platelet counts) reveal that it has little influence on blood within 15 days of administration. When injected into HeLa xenograft nude mice by the tail vein, the probe elicited intensely enhanced fluorescence and X-ray computed tomography (CT) signals in the tumors after 24 hours, and the structure, size and position of tumor tissue were shown clearly. In a word, the probe has good tumor targeting capabilities, and it has significant value in fluorescence-CT dual-mode imaging in vivo.


Assuntos
Iodo , Neoplasias Experimentais , Óleos , Imagem Óptica/métodos , Pontos Quânticos , Compostos de Prata , Tomografia Computadorizada por Raios X/métodos , Animais , Feminino , Células HeLa , Humanos , Iodo/farmacocinética , Iodo/farmacologia , Células MCF-7 , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Neoplasias Experimentais/sangue , Neoplasias Experimentais/patologia , Óleos/farmacocinética , Óleos/farmacologia , Compostos de Prata/farmacocinética , Compostos de Prata/farmacologia
15.
ACS Appl Mater Interfaces ; 7(31): 17287-97, 2015 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-26189815

RESUMO

We developed a simple and efficient method to synthesize a novel probe for both computed tomography (CT) and fluorescence imaging. Gold nanospheres were coated with positively charged mesoporous silica (Au@mSiO2-TTA) using a one-pot method to cohydrolyze quaternary ammonium silane and tetraethyl orthosilicate. Subsequently, IR-783, a negatively charged and water-soluble near-infrared fluorescent dye, was electrostatically adsorbed into the silica shell. Transmission electron microscopy imaging, X-ray powder diffraction, and energy dispersive X-ray spectroscopy indicated that Au@mSiO2-TTA had a clear core-shell structure, was monodisperse, had a large surface area (530 m2/g), and had a uniform pore size (2.2 nm). The mesoporous structure could effectively load fluorescent dye. After loading, the zeta potential of the nanoparticle dropped from 48 mV to 30 mV, and after additional modification with polyvinylpyrrolidone, it further reduced to 6 mV. Probe fluorescence was stable over time, and the probe was an effective CT contrast agent and as a near-infrared fluorescent probe. The half-life of the probe in the blood was 1.5 h, and the probe was mainly distributed in the spleen and liver 4 h after injection. Tissue sections showed that major organs were normal and without visible morphological changes, 6 days post injection, indicating the biocompatibility of the probe.


Assuntos
Corantes Fluorescentes/química , Ouro/química , Nanosferas/química , Dióxido de Silício/química , Tomografia Computadorizada por Raios X , Animais , Linhagem Celular , Meios de Contraste/química , Meios de Contraste/farmacocinética , Meia-Vida , Raios Infravermelhos , Camundongos , Camundongos Nus , Porosidade , Povidona/química , Espectrometria por Raios X , Espectroscopia de Luz Próxima ao Infravermelho , Distribuição Tecidual
16.
Dalton Trans ; 44(25): 11314-20, 2015 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-26008798

RESUMO

In this study, silica coated Au nanospheres (Au@SiO2) were prepared by a reverse microemulsion method; subsequently, a layer of fluorescent quantum dots (QDs) were adsorbed onto it and then it was coated with silica again. After modifying with PVP, the composite silica coated gold nanosphere and quantum dots nanoparticle (Au@SiO2-QDs/SiO2-PVP) was obtained. This composite structure contained Au and QDs, and it could be used for contrast-enhanced X-ray CT imaging and fluorescence imaging. Characterization showed that the composite nanoparticle had good dispersity, a high fluorescence intensity and a good effect of X-ray absorption, and it was suitable for using as a bimodal imaging probe.


Assuntos
Corantes Fluorescentes/química , Ouro/química , Nanopartículas/química , Pontos Quânticos/química , Dióxido de Silício/química , Animais , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Meios de Contraste/química , Meios de Contraste/toxicidade , Diatrizoato de Meglumina/química , Diatrizoato de Meglumina/toxicidade , Emulsões , Corantes Fluorescentes/toxicidade , Ouro/toxicidade , Camundongos , Nanopartículas/toxicidade , Nanopartículas/ultraestrutura , Imagem Óptica , Povidona/química , Pontos Quânticos/toxicidade , Dióxido de Silício/toxicidade , Espectroscopia de Infravermelho com Transformada de Fourier , Tomografia Computadorizada por Raios X
17.
Org Biomol Chem ; 13(11): 3457-61, 2015 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-25666380

RESUMO

A PhI(OAc)2-promoted radical cyclization of ß,γ-unsaturated hydrazones and oximes has been developed for an efficient synthesis of various valuable pyrazoline and isoxazoline derivatives with satisfactory yields (up to 96%) under mild conditions.


Assuntos
Acetatos/química , Alcenos/química , Iodobenzenos/química , Isoxazóis/síntese química , Pirazóis/síntese química , Isoxazóis/química , Estrutura Molecular , Pirazóis/química
18.
BMC Infect Dis ; 15: 34, 2015 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-25636581

RESUMO

BACKGROUND: After more than 10 years without a case of wild poliovirus (WPV) in China, an outbreak occurred in 2011 in Xinjiang Uyghur Autonomous Region. METHODS: Acute flaccid paralysis (AFP) case surveillance was strengthened with epidemiological investigations and specimen collection and serological surveys were conducted among hospitalized patients. RESULTS: There were 21 WPV cases and 23 clinical compatible polio cases reported. WPV was isolated from 14 contacts of AFP cases and 13 in the healthy population. Incidence of WPV and clinical compatible polio cases were both highest among children <1 years, however, 24/44 (54.5%) polio cases were reported among adults aged 15-39 years. CONCLUSIONS: High coverage of routine immunization should be maintained among children until WPV transmission is globally eradicated. Expansion of AFP case surveillance and use of serologic surveys to estimate population immunity should be conducted rapidly to guide preparedness and response planning for future WPV outbreaks.


Assuntos
Surtos de Doenças , Poliomielite/epidemiologia , Adolescente , Adulto , Distribuição por Idade , Criança , Pré-Escolar , China/epidemiologia , Busca de Comunicante , Surtos de Doenças/prevenção & controle , Feminino , Humanos , Incidência , Lactente , Recém-Nascido , Masculino , Poliomielite/diagnóstico , Poliomielite/prevenção & controle , Vigilância em Saúde Pública , Estudos Retrospectivos , Adulto Jovem
19.
BMC Infect Dis ; 15: 18, 2015 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-25595618

RESUMO

BACKGROUND: This report describes emergency response following an imported vaccine derived poliovirus (VDPV) case from Myanmar to Yunnan Province, China and the cross-border collaboration between China and Myanmar. Immediately after confirmation of the VDPV case, China disseminated related information to Myanmar with the assistance of the World Health Organization. METHODS: A series of epidemiological investigations were conducted, both in China and Myanmar, including retrospective searches of acute flaccid paralysis (AFP) cases, oral poliovirus vaccine (OPV) coverage assessment, and investigation of contacts and healthy children. RESULTS: All children <2 years of age had not been vaccinated in the village where the VDPV case had lived in the past 2 years. Moreover, most areas were not covered for routine immunization in this township due to vaccine shortages and lack of operational funds for the past 2 years. CONCLUSIONS: Cross-border collaboration may have prevented a potential outbreak of VDPV in Myanmar. It is necessary to reinforce cross-border collaboration with neighboring countries in order to maximize the leverage of limited resources.


Assuntos
Surtos de Doenças/prevenção & controle , Poliomielite/prevenção & controle , Vacina Antipólio Oral/provisão & distribução , Poliovirus/imunologia , Criança , Pré-Escolar , China/epidemiologia , Comportamento Cooperativo , Emigração e Imigração , Feminino , Humanos , Lactente , Masculino , Mianmar/epidemiologia , Estudos Retrospectivos , Vacinação , Organização Mundial da Saúde
20.
Phytother Res ; 29(2): 220-7, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25312586

RESUMO

Radix Angelicae Dahuricae is the dried root of Angelicae Dahurica (Fisch.ex Hoffm.)Benth.et Hook.f. var.formosana (Boiss.) Shan et Yuan (Fam.Umbelliferae). The total coumarins (Cou) and volatile oil (VO) were main active components that drived from Radix Angelicae Dahuricae. Our previous studies have shown that Cou and VO could increase intestinal absorption for transmucosal drug delivery with unknown mechanism. The aim of this study was to investigate the molecular mechanism of Radix Angelicae Dahuricae for improving drug intestinal transport. Caco-2 cell model was used to study the effect of Radix Angelicae Dahurica on transepithelial electrical resistance. Western blot was used to study its effect on the expression of the actin and ZO-1, tight junction proteins. The effect of Radix Angelicae Dahurica on the expression of P-gp protein was investigated using flow cytometry. VO (0.036-2.88 µL/mL) and Cou (0.027-0.54 mg/mL) caused a reversible, time- and dose-dependent decrease in transepithelial electrical resistance. VO and/or Cou could inhibit the expression of the tight junction protein, ZO-1 and actin. VO and/or Cou also could inhibit the expression of P-gp. These data suggested that Radix Angelicae Dahurica increased cell permeability by affecting the expression of actin, ZO-1 or P-gp, opening the tight junction or inhibiting the efflux induced by P-gp.


Assuntos
Angelica/química , Medicamentos de Ervas Chinesas/farmacologia , Flavonoides/farmacocinética , Absorção Intestinal/efeitos dos fármacos , Isoflavonas/farmacocinética , Membro 1 da Subfamília B de Cassetes de Ligação de ATP/metabolismo , Actinas/metabolismo , Transporte Biológico/efeitos dos fármacos , Células CACO-2 , Cumarínicos/farmacologia , Humanos , Mucosa Intestinal/metabolismo , Óleos Voláteis/farmacologia , Raízes de Plantas/química , Junções Íntimas/efeitos dos fármacos , Proteína da Zônula de Oclusão-1/metabolismo
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