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1.
J Sep Sci ; 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-32227667

RESUMO

In this paper, four types of middle-pressure chromatogram isolated gels are evaluated for adsorption/desorption characteristics of ginsenosides from Panax ginseng. Among them, SP207SS and SP2MGS were selected for dynamic investigations based on their static adsorption/desorption capacity of total ginsenoside. Their adsorption kinetics was better explained by pseudo-second-order model and isotherms were preferably fitted to Langmuir model. Dynamic breakthrough experiments indicated an optimum sample loading speed of 4BV/h for either SP207SS or SP2MGS. Desorption speed was determined to be 2BV/h according to desorption amount of total ginsenoside in their effluents. Eight ginsenosides were identified and quantified by HPLC-TQ-MS in total ginsenoside extract and different fractions during stepwise dynamic elution. For SP207SS, 27.62% of loaded ginsenosides was detected in 40% ethanol fraction, while 59.12% of them were found in 60% ethanol fraction. As on SP2MGS, the number went to 53.71% and 44.43% respectively. Recovery rate of ginsenosides were calculated to 78.65% for SP207SS and 89.53% for SP2MGS. Intriguingly, content of Rg1 and Re in 40% ethanol fraction from SP207SS became 20.1 and 18.6 times higher than that in total ginsenoside extract by one step elution, which could be leveraged for the facile enrichment of these two ginsenosides from natural sources. This article is protected by copyright. All rights reserved.

2.
J Gen Virol ; 2020 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-32149597

RESUMO

Pepper mild mottle virus (PMMoV) causes serious economic losses in pepper production in China. In a survey for viral diseases on pepper, two PMMoV isolates (named PMMoV-ZJ1 and PMMoV-ZJ2) were identified with different symptoms in Zhejiang province. Sequence alignment analysis suggested there were only four amino acid differences between the isolates: Val262Gly, Ile629Met and Ala1164Thr in the replicase, and Asp20Asn in the coat protein. Infectious cDNA clones of both isolates were constructed and shown to cause distinctive symptoms. Chlorosis symptoms appeared only on PMMoV-ZJ2-infected plants and the Asp20Asn substitution in the CP was shown to be responsible. Confocal assays revealed that the subcellular localization pattern of the two CPs was different, CP20Asp was mainly located at the cell periphery, whereas most CP20Asn located in the chloroplast. Thus, a single amino acid in the CP determined the chlorosis symptom, accompanied by an altered subcellular localization.

3.
Chem Commun (Camb) ; 2020 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-32167512

RESUMO

Herein, we highlighted fluorine-anion-modification as a useful method for enhancing OER catalytic activity. Fluorine-anion-doped Ni foam exhibits an almost 10-fold enhancement in OER catalytic activity. Moreover, fluorine-anion-modification could be used as a general method to greatly increase the OER activity of NiAl LDHs. The strongly electronegative F anion is favorable to build weak metal-fluorine bonds, which easily break to form active species of nickel oxides/hydroxides, enhancing OER catalytic performance.

4.
Artigo em Inglês | MEDLINE | ID: mdl-32159381

RESUMO

Objective: This study aimed at investigating the specific roles of laminarin from seaweed (Laminaria japonica) in hepatocellular carcinoma (HCC) and its potential mechanisms related to senescence marker protein-30 (SMP-30). Materials and Methods: Human HCC cell lines, including Bel-7404 and HepG2, were incubated with different concentrations of laminarin (0, 5, 15, 25, 35, and 45 mg/mL). The cell viability and apoptosis rates were detected by WST-8 cell proliferation assay and flow cytometry, respectively. Hepa 1-6 tumor-bearing mice were injected with different concentrations of laminarin (400, 800, and 1200 mg/kg·d), and tumor volume and weight were measured. The expression of SMP-30 was detected in laminarin-treated Bel-7404 and HepG2 HCC cells and LO2 normal liver cells by quantitative real-time PCR and Western blotting. Results: The treatment with laminarin (48 h) significantly decreased the viability and increased the apoptosis rates of Bel-7404 and HepG2 cells in a dose-dependent manner. The injection of laminarin also significantly decreased the tumor volumes (beginning on the 10th day) and tumor weights (30 d post-injection) of mice in a dose-dependent manner. In addition, the treatment with laminarin (35 mg/mL for 48 h) significantly upregulated SMP-30 in Bel-7404 and HepG2 cells but not in LO2 cells. Conclusion: Laminarin inhibited the proliferation of Bel-7404 and HepG2 cells and inhibited the growth of tumors in Hepa 1-6 tumor-bearing mice by upregulating SMP-30.

5.
Oncol Lett ; 19(4): 3123-3136, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32218863

RESUMO

Non-coding RNAs (ncRNAs) regulate numerous genes and influence the progression of various human diseases, including cancer. The role of regulatory ncRNAs implicated in nasopharyngeal carcinoma (NPC), as well as their target genes, remains unclear. The present study aimed to investigate specific long non-coding (lnc)RNAs, circular RNAs (circRNAs) and mRNAs associated with the molecular pathogenesis of NPC, and to predict the underlying target genes of specific lncRNAs and circRNAs. The expression levels of lncRNAs, circRNAs and mRNAs in NPC and chronic nasopharyngitis tissues were detected and analyzed using microarray and bioinformatics techniques. A total of 2.80% lncRNAs (425 upregulated and 431 downregulated) were significantly differentially expressed (DE) between the two tissue types. Additionally, 0.96% circRNAs (18 upregulated and 13 downregulated) were significantly DE, while 2.94% mRNAs (426 upregulated and 341 downregulated) were significantly DE between the two tissue types. In total, 420 NPC-associated nearby encoding genes (196 up- and 224 downregulated) of the DE lncRNAs were identified. Overlap analysis identified 23 DE circRNAs and their corresponding target genes, with 37 microRNAs and 50 mRNAs, from which 14 interaction networks were constructed. Subsequent pathway analysis revealed 221 DE target genes corresponding to 31 key signaling pathways associated with NPC, 14 of which may represent hub genes associated with NPC pathophysiology. Thus, certain lncRNAs, circRNAs and mRNAs are aberrantly expressed in NPC tissues, and partially specific lncRNAs, circRNAs and their target genes may influence the tumorigenesis and progression of NPC. Target prediction and regulatory network identification may help to determine the pathogenic mechanisms of NPC.

6.
EBioMedicine ; 53: 102689, 2020 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-32114396

RESUMO

BACKGROUND: How the oncoprotein epidermal growth factor receptor (EGFR) evades proteolytic degradation and accumulates in non-small cell lung cancer (NSCLC) remains unclear, and ubiquitin pathway genes (UPGs) that are critical to NSCLC needs to be systematically identified. METHODS: A total of 696 UPGs (including E1, E2, E3, and deubiquitinases) were silenced by small interfering RNA (siRNA) library in NSCLC cells, the candidates were verified, and their significance was evaluated in patients with NSCLC. The effects of a candidate gene on EGFR were investigated in vitro and in vivo. FINDINGS: We report 31 candidates that are required for cell proliferation, with the E2 ubiquitin conjugase CDC34 as the most significant one. CDC34 is elevated in tumor tissues in 76 of 114 (66.7%) NSCLCs and inversely associated with prognosis, is higher in smoker patients than nonsmoker patients, and is induced by tobacco carcinogens in normal human lung epithelial cells. Forced expression of CDC34 promotes, whereas knockdown of CDC34 inhibits, NSCLC cell proliferation in vitro and in vivo. CDC34 competes with c-Cbl to bind Y1045 to inhibit polyubiquitination and degradation of EGFR. In EGFR-L858R and EGFR-T790M/Del (exon 19)-driven lung tumor growth in mouse models, knockdown of CDC34 significantly inhibits tumor formation. INTERPRETATION: These results demonstrate that an E2 enzyme is capable of competing with E3 ligase to stabilize substrates, and CDC34 represents an attractive therapeutic target for NSCLCs. FUNDING: National Key Research and Development Program of China, National Natural Science Foundation of China, and the CAMS Innovation Fund for Medical Sciences.

7.
Chem Commun (Camb) ; 2020 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-32211647

RESUMO

A halide perovskite based photocatalyst has been demonstrated for the first time to simultaneously achieve efficient photocatalytic CO2 reduction and methanol oxidation, exhibiting an exciting yield of 1835 µmol g-1 for photocatalytic CO2-to-CO conversion. Moreover, almost stoichiometric value-added formic acid can be produced from methanol oxidation.

8.
Talanta ; 212: 120769, 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-32113539

RESUMO

Quantitative detection of phospholipids at the single cell level remains in challenge. Herein, the TiO2-coated Fe3O4 nanoparticles were synthesized to selectively enrich trace phospholipids from single cell, which were then eluted using 1.5% ammonia/methanol (w/w) for sensitive detection by electrospray ionization mass spectrometry. Under the optimal experimental conditions, eighteen phospholipids in single cell samples were detected and identified by MS/MS experiments. The limit-of-detections (LODs) were 0.012 µg/L for phosphatidylcholine (PC, 34:1) and 0.014 µg/L for phosphatidylcholine (PC, 36:2) in PBS matrix, with the linear range of 0.05-50 µg/L (R2 ≥ 0.999). The recovery rates of 94.90-104.00% were obtained, with the relative standard deviations (RSDs ≤ 6.90%). Quantitative determination of PC in real unicellular samples was also achieved, with the concentration of 1.82-2.11 µg/L for PC(34:1) and 1.25-1.65 µg/L for PC(36:2) in six types of single cell, opening up possibilities for quantitative analysis of trace compounds in complex bio-samples. A set of 6 types of tumor cells were analyzed and further differentiated by the partial least squares-discriminant analysis (PLS-DA). Conclusively, a facile method for the direct quantification of phospholipids in single cell samples has been developed, showing potential applications for advanced investigation of phosphorylated substance at the single cell level.

9.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 28(1): 40-50, 2020 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-32027251

RESUMO

OBJECTIVE: To investigate the correlation of single nucleotide polymorphisms (SNP) in arachidonate 5-lipoxygenase gene (ALOX5) rs2029253, rs2228064 and rs2228065 sites, 5-lipoxygenase activating protein gene (ALOX5AP) rs10507391, rs4769874 sites with the risk for genesis of adult myeloid leukemia. METHODS: By the approval from the hospital ethics committee and the informed consent of participants. 150 patients with myeloid leukemia (ML) as ML group and 134 healthy people as the control group were selected. The genomic DNA was extracted from the samples. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) combined with directly sequencing, PCR-amplified products were applied to test the polymorphism of 5 sites in ALOX5 and ALOX5AP gene. RESULTS: A allele frequencies of ALOX5 gene rs2029253 site in the ML group and the control group were 43.0% and 34.3%, respectively. And the G allele frequencies in the ML group and the control group were 57.0% and 65.7%, respectively. The genotype distributions of AA, AG and GG in ALOX5 gene rs2029253 site in the ML group were 32.2%, 21.5% and 46.3% respectively. That in the control group were 15.7%, 37.3% and 47.0% respectively. The genotype AA and A allele frequency of ALOX5 gene rs2029253 site were linked with the increased risk of myeloid leukemia (OR=2.26, 95% CI: 1.43-4.56, P<0.05; OR=1.44, 95% CI: 1.02-2.03, P<0.05). And the genotype AG and allele G reduced the susceptibility to myeloid leukemia (OR=0.46, 95% CI: 0.27-0.78, P<0.01; OR=0.69, 95% CI: 0.50-0.98, P<0.05), however, the polymorphisms of ALOX5 gene rs2228064 and rs2228065 site not correlated with the risk of myeloid leukemia (P>0.05). The A allele frequency of ALOX5AP gene rs10507391 site in the ML group and the control group were 30.7% and 36.2% respectirely. The genotype distribution rates of AA, AT and TT in ALOX5AP gene rs10507391 site in the ML group was 1.3%, 58.7% and 40.0% respectively, that in the control group were 9.7%, 53.0% and 37.3% respectively. The genotype AA of ALOX5AP gene rs10507391 site correlated with the decreased risk of myeloid leukemia (OR=0.13, 95% CI: 0.03-0.57, P<0.05), but the polymorphism of ALOX5AP gene rs4769874 site not correlated with the risk of myeloid leukemia (P>0.05). CONCLUSION: The genotype AA, AG and allele A, G of ALOX5 rs2029253, as well as ALOX5AP rs10507391 may be correlate with the susceptibility to myeloid leukemia.


Assuntos
Proteínas Ativadoras de 5-Lipoxigenase/genética , Araquidonato 5-Lipoxigenase/genética , Leucemia Mieloide , Adulto , Estudos de Casos e Controles , Frequência do Gene , Predisposição Genética para Doença , Genótipo , Humanos , Leucemia Mieloide/genética , Polimorfismo de Nucleotídeo Único , Fatores de Risco
10.
Int J Biol Macromol ; 149: 572-580, 2020 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-32006581

RESUMO

Spent coffee grounds (SCG) are a promising raw material for galactomannan (GalM) production based upon its enrichment in galactomannan polysaccharides. In this work, SCG was pretreated by autohydrolysis for maximumly improving GalM extractability by endo-mannanase. The GalM in the prehydolyzate (GalM-PH) and enzymatic hydrolyzate (GalM-EH) were obtained by ethanol precipitation and characterized. Under the optimized autohydrolysis conditions, 50.1% of GalM in pretreated SCG was converted into free GalM in enzymatic hydrolyzate. Compositional analysis results revealed that GalM-PH was comprised of 81.7% galactomannan, higher than that of GalM-EH (76.4%). The molecular weight of GalM-PH and GalM-EH were 44.5 kDa and 28.0 kDa, respectively. Antioxidant assays indicated that both GalM-EH and GalM-PH could scavenge 2,2-diphenyl-1-picryl-hydrazyl radicals and hydroxyl radicals. Immunological and prebiotics analysis showed all GalM preparations exhibited pronounced activities for proliferating the probiotics and proliferating the Macrophages cell for NO production, in which the GalM-EH outperformed the GalM-PH. These results imply that the GalM extracted from SCG are the bioactive substances that can be used as antioxidant, prebiotics, and immunostimulants.

12.
Bioorg Med Chem ; 28(7): 115358, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32081628

RESUMO

PET imaging of α-synuclein (α-syn) deposition in the brain will be an effective tool for earlier diagnosis of Parkinson's disease (PD) due to α-syn aggregation is the widely accepted biomarker for PD. However, the necessary PET radiotracer for imaging is clinically unavailable until now. The lead compound discovery is the first key step for the study. Herein, we initially established an efficient biologically evaluation system well in highthroughput based on SPR technology, and identified a novel class of N, N-dibenzylcinnamamide (DBC) compounds as α-syn ligands through the assay. These compounds were proved to have high affinities against α-syn aggregates (KD < 10 nM), which well met the requirement of binding activity for the PET probe. These DBC compounds were firstly reported as α-syn ligands herein and the preliminary obtained structure has been further modified into F-labeled ones. Among them, a high-affinity tracer (5-41) with 1.03 nM (KD) has been acquired, indicating its potential as a new lead compound for developing PET radiotracer.

13.
Bioorg Med Chem Lett ; 30(6): 126982, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-32001137

RESUMO

This work explored a novel type of potential multi-targeting antimicrobial three-component sulfanilamide hybrids in combination of pyrimidine and azoles. The hybridized target molecules were characterized by 1H NMR, 13C NMR and HRMS spectra. Some of the developed target compounds exerted promising antimicrobial activity in comparison with the reference drugs norfloxacin and fluconazole. Noticeably, sulfanilamide hybrid 5c with pyrimidine and indole could effectively inhibit the growth of E. faecalis with MIC value of 1 µg/mL. The active molecule 5c showed low cell toxicity and did not obviously trigger the development of resistance towards the tested bacteria strains. Mechanism exploration indicated that compound 5c could not only exert efficient membrane permeability, but also intercalate into DNA of resistant E. faecalis to form 5c-DNA supramolecular complex, which might be responsible for its antimicrobial action. The further investigation showed that this molecule could be effectively transported by human serum albumins through hydrogen bonds and van der Waals force.

14.
Chin Med J (Engl) ; 133(5): 542-551, 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-32053571

RESUMO

BACKGROUND: The eosinophilic chronic obstructive pulmonary disease (COPD) is known to be more sensitive to corticosteroid. The sputum microbiome has been shown to affect COPD prognosis, but its role in acute exacerbations of eosinophilic COPD is unclear. This study aimed to investigate the dynamic changes of the airway microbiome in patients with acute exacerbations of eosinophilic COPD. METHODS: Fifty-seven patients with acute exacerbations of COPD from the First Affiliated Hospital of Guangxi Medical University between June 2017 and June 2018 were divided into two groups. Patients with eosinophils ≥300 cells/µL in the peripheral venous blood were assigned to the eosinophilic group (Eos) and the rest to the non-eosinophilic group (Noneos). All patients received similar treatment including inhaled budesonide according to the guidelines. The induced sputum microbiome was analyzed on the 1st and 7th day of treatment using the 16S ribosomal RNA (rRNA) method. The levels of interleukin (IL)-6 and IL-8 were measured in the plasma and the sensitivity to corticosteroids was determined in isolated peripheral blood mononuclear cells. Quantitative data were compared between the two groups using the independent samples t test or Mann-Whitney U test. Categorical data were evaluated using Chi-squared test or Fisher's exact test. RESULTS: Twenty-six patients were classified into Eos group and 31 patients were classified into Noneos group. Prior to treatment, the alpha diversity (Shannon index) (2.65 ±â€Š0.63 vs. 2.56 ±â€Š0.54, t = 0.328, P = 0.747) and the structure of the sputum microbiome were similar in the Eos group and the Noneos group. After 7 days of treatment, alpha diversity increased in both groups, while the microbiome richness (Ace index) was significantly lower in the Eos group (561.87 ±â€Š109.13 vs. 767.88 ±â€Š148.48, t = -3.535, P = 0.002). At the same time, IL-6 (12.09 ±â€Š2.85 pg/mL vs. 15.54 ±â€Š2.45 pg/mL, t = -4.913, P < 0.001) and IL-8 (63.64 ±â€Š21.69 pg/mL vs. 78.97 ±â€Š17.13 pg/mL, t = -2.981, P = 0.004) decreased more significantly in the Eos group, and the percentages of inhibition of IL-8 at dexamethasone concentrations 10 to 10 mol/L were significantly higher in the Eos group than those in the Noneos group (all P < 0.05). CONCLUSIONS: The induced sputum microbiome richness decreased more significantly following treatment in the Eos patients compared to the Noneos patients. The lower plasma inflammatory factor levels and the higher percentage of inhibition of IL-8 might be due to higher corticosteroid sensitivity in Eos patients.

15.
Reprod Sci ; 27(1): 325-333, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32046389

RESUMO

Anti-Müllerian hormone (AMH) downregulates the level of stem cell factor (SCF) via the cAMP/PKA signaling pathway in human granulosa cells (GCs). Little information is available on the molecular mechanism underlying the interaction. This study is aimed at determining whether AMH regulates expression of SCF via the cAMP-PKA-CREB signaling pathway in human GCs. In the present study, we verified the binding of cAMP-response element-binding protein (CREB) to promoter of SCF in human GCs. Furthermore, the effect of CREB was tested on the SCF promoter, and the site of CREB binding to SCF promoter was identified using truncations as well as assays of SCF-promoted mutation and CREB mutation. To investigate the correlation among AMH, SCF promoter, and CREB, pGL-Basic-SCF+CREB was transfected into overexpressed AMH GCs (AMH-high GCs), low expressed AMH GCs (AMH-low GCs), and normal GCs (GCs), respectively. Finally, immunofluorescence, double immunostaining, and Western blot were carried out in AMH-high and AMH-low GCs to confirm the AMH-mediated regulation of SCF expression by inhibiting the phosphorylation of CREB (pCREB) in GCs. Results indicated CREB interacted with SCF promoter and significantly enhanced the transcription level of SCF. The CREB binding site was localized at 318-321 bp of SCF gene promote. AMH inhibits the expression of SCF by phosphorylation of CREB via the PKA signaling pathway in GCs. These findings provide an in-depth understanding of the molecular mechanism underlying AMH suppressing the follicle growth, which would aid in the development of a novel therapy.

16.
J Mater Chem B ; 2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-31932835

RESUMO

This work reports an enzyme-free glucose sensor based on nickel nanostructures electrodeposited on a fluorine-doped tin oxide (FTO) electrode modified with a silica nanochannel membrane (SNM). The SNM consists of a high density of nanochannels vertically oriented to the electrode surface, which can spatially confine the electrodeposition of nickel nanostructures and protect them to make Ni@SNM/FTO electrodes. In alkaline media, nickel could be converted to nickel oxyhydroxide that displayed catalytic activity toward the anodic oxidation of glucose. The electrodes could thus function as enzyme-free sensors for glucose detection. Under optimal conditions, the sensors exhibited an excellent analytical performance, with an analytical sensitivity as high as 62.3 µA mM-1 cm-2, a wide detection range from 10 µM to 12 mM and a low detection limit of 0.44 µM. Furthermore, given nickel nanostructures were embedded inside the nanochannels of the SNM (with a diameter of 2-3 nm), the sensor possessed anti-fouling ability and outstanding current stability, thus allowing the direct detection of glucose in dilute blood samples.

17.
Phys Rev Lett ; 124(1): 014801, 2020 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-31976698

RESUMO

Generation of circularly polarized (CP) and linearly polarized (LP) γ rays via the single-shot interaction of an ultraintense laser pulse with a spin-polarized counterpropagating ultrarelativistic electron beam has been investigated in nonlinear Compton scattering in the quantum radiation-dominated regime. For the process simulation, a Monte Carlo method is developed which employs the electron-spin-resolved probabilities for polarized photon emissions. We show efficient ways for the transfer of the electron polarization to the high-energy photon polarization. In particular, multi-GeV CP (LP) γ rays with polarization of up to about 95% can be generated by a longitudinally (transversely) spin-polarized electron beam, with a photon flux meeting the requirements of recent proposals for the vacuum birefringence measurement in ultrastrong laser fields. Such high-energy, high-brilliance, high-polarization γ rays are also beneficial for other applications in high-energy physics, and laboratory astrophysics.

18.
Artigo em Inglês | MEDLINE | ID: mdl-31985528

RESUMO

PURPOSE: A negative relationship between intraocular vascular endothelial growth factor-A (VEGFA) and axial length was found, which may help explain why myopia with long axial length was a protective factor for development of diabetic retinopathy (DR). The aim of this study is to further assess the relationship between the aqueous humor levels of interlukin (IL)-8, IL-10, VEGFA, vascular adhesion molecule-1 (VCAM-1), basic fibroblast growth factor, VEGFB, and placental growth factor (PLGF) and axial length in eyes with DR. DESIGN: Retrospective, single-center, unmasked study. METHODS: Patients with age-related cataract and with/without DR who visited the Department of Ophthalmology at the Affiliated Hospital of Inner Mongolia Medical University were enrolled. The level of IL-8, IL-10, VEGFA, VCAM-1, and basic fibroblast growth factor were measured by cytometric bead array, and VEGFB and PLGF were measured by enzyme-linked immunosorbent assay. Axial length was measured by biometry. RESULTS: Totally 65 eyes of 65 patients were enrolled, including 14 patients with nonproliferative diabetic retinopathy, 16 patients with proliferative diabetic retinopathy (PDR), and 35 patients with age-related cataract as control. In the nonproliferative diabetic retinopathy group, the aqueous level of PLGF was negatively correlated with axial length (r = -0.576, P = 0.031), whereas the aqueous levels of IL-10 (r = 0.533, P = 0.049) and VCAM-1 (r = 0.566, P = 0.035) were positively correlated with axial length. In the proliferative diabetic retinopathy group, all cytokines did not significantly correlate with axial length. CONCLUSIONS: Among patients with diabetic retinopathy, we further found that aqueous levels of PLGF were negatively correlated with axial length, whereas VCAM-1 and IL-10 were positively correlated with axial length. These findings may suggest that these cytokines play a role in the development of DR, and further explain the relationship between the axial length and DR.

19.
Cell Cycle ; 19(3): 317-325, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31918603

RESUMO

We carried out this study to unravel the function of Litchi Seed Aqueous Extracts (LSAE) on biological functions of breast cancer (BC) cells. MTT assay was adopted to measure proliferation of BC cells (MCF7, BT474 and MDA-MB-231) and normal mammary cells (MCF10A) under different time points (24, 48 and 72 h) and different concentrations (50, 100, 200 and 400 µg/mL). MCF-7 cells were selected for subsequent experiments and were grouped into blank group, negative control (NC) group, low-, medium- and high-dose LSAE (L-LSAE, M-LSAE, H-LSAE) groups. Cell viability, invasion, migration and apoptosis were measured by functional assays. Low dosage of LSAE (50 and 100 µg/mL) enhanced proliferation of MCF10A cells, while high dosage of LSAE (200 and 400 µg/mL) suppressed proliferation of MCF10A cells. The proliferation inhibition rate in BT474 and MDA-MB-231cells was increased relative to that in MCF7 cells. MCF-7 cells in the L-LSAE, M-LSAE and H-LSAE groups were rounded and epithelial-like, in which cell survival rate, epithelial-mesenchymal transition (EMT), invasion and migration abilities were reduced versus the blank and NC groups. The tendency in the H-LSAE group was substantially obvious than those in the L-LSAE and M-LSAE groups (both P < 0.05). We found that LSAE is able to inhibit EMT, invasion and migration in BC cells based on concentration and time.

20.
Plant Physiol ; 182(1): 204-214, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31694901

RESUMO

MicroRNAs (miRNAs) are small noncoding RNAs of ∼21 nt in length, which have regulatory roles in many biological processes. In animals, proper functioning of the circadian clock, which is closely linked to the fitness of almost all living organisms, is regulated by miRNAs. However, to date, there have been no reports of the roles of miRNA in regulation of the plant circadian rhythm. Here, we report a natural variant of miR397 that lengthens the circadian period and controls flowering time in Arabidopsis (Arabidopsis thaliana). Highly conserved among angiosperms, the miRNA miR397 has two members in Arabidopsis: miR397a and miR397b. However, only miR397b significantly delayed flowering. Our results suggest that miR397b controls flowering by targeting CASEIN KINASE II SUBUNIT BETA3 (CKB3), in turn modulating the circadian period of CIRCADIAN CLOCK ASSOCIATED1 (CCA1). We further demonstrated that CCA1 directly bound to the promoter of MIR397B and suppressed its expression, forming a miR397b-CKB3-CCA1 circadian regulation feedback circuit. Evolutionary analysis revealed that miR397b is a newly evolved genetic variant in Arabidopsis, and the miR397b targeting mode may have a role in enhancing plant fitness. Our results provide evidence for miRNA-mediated circadian regulation in plants and suggest the existence of a feedback loop to manipulate plant flowering through the regulation of circadian rhythm.

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