Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 2.058
Filtrar
1.
Artigo em Inglês | MEDLINE | ID: mdl-33821442

RESUMO

Microwave-assisted catalytic oxidation technology has become an effective technology for rapid removal of organic pollutants in wastewater. In this research, the removal of methyl orange (MO) from aqueous solution by CuFe2O4 loaded on carbon nanotubes (CuFe2O4/CNTs) under microwave irradiation was studied. The effects of different loadings (1:2, 1:4, 1:8) of CuFe2O4 on the dielectric loss, magnetic loss, dielectric loss factor, magnetic loss factor, and reflection loss of composite materials were studied. The results showed that the microwave adsorption performance was improved by loading CuFe2O4 on CNTs. These different composites were further characterized by SEM, FTIR, and XRD techniques. In addition, this article also studied the effects of different microwave irradiation time, pH, and ionic factors on the degradation of MO. In particular, the mechanism of MO degradation by composite materials under different pH conditions was also studied in detail. The results showed that the removal rate reaches 97% with 5 min under the best conditions, and the composite material had good anti-interference performance. This study may provide a new option to degrade organic dye in wastewater treating.

2.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 29(2): 322-327, 2021 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-33812394

RESUMO

OBJECTIVE: To construct an acute myeloid leukemia cell line stably expressing CD123-CLL1 so as to provide an "in vitro" model for studying the role of CD123 and CLL-1 in leukemia and the treatment targeting CD123 and CLL-1. METHODS: The recombinant plasmid of lentivirus was constructed by synthesizing CD123 and CLL-1 sequences and PCR homologous recombination. The lentivirus vector was packaged by three-plasmid packaging system. After collecting the supernatant of lentivirus, the virus titer was determined by quantitative PCR. K562 leukemia cells were collected and transtected with virus supernatant. Leukemia cell line stably expressing the target gene were screened by purinomycin. The expression levels of CD123 and CLL-1 were detected by RT-PCR and flow cytometry. RESULTS: The lentiviral vector was successfully constructed, and identified by agarose gel electrophoresis and gene sequencing, then the virus titer of the supernatant was up to 5.81×108 after quantitative PCR assay. The K562 leukemia cell line obtained positive expression cells after being infected by puromycin. The high expression of CD123 and CLL-1 was confirmed by RT-PCR, while the significantly high expression of CD123 and CLL-1 was confirmed by flow cytometry. CONCLUSION: Lentiviral vector expressing CD123-CLL1 has been successfully constructed, and K562 leukemia cell line stably expressing CD123 and CLL-1 has been successfully obtained.


Assuntos
Subunidade alfa de Receptor de Interleucina-3 , Leucemia Linfocítica Crônica de Células B , Linhagem Celular Tumoral , Vetores Genéticos , Humanos , Células K562 , Lentivirus/genética , Leucemia Linfocítica Crônica de Células B/genética , Plasmídeos , Transfecção
3.
Sensors (Basel) ; 21(5)2021 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-33802421

RESUMO

The vast amounts of mobile communication data collected by mobile operators can provide important insights regarding epidemic transmission or traffic patterns. By analyzing historical data and extracting user location information, various methods can be used to predict the mobility of mobile users. However, existing prediction algorithms are mainly based on the historical data of all users at an aggregated level and ignore the heterogeneity of individual behavior patterns. To improve prediction accuracy, this paper proposes a weighted Markov prediction model based on mobile user classification. The trajectory information of a user is extracted first by analyzing real mobile communication data, where the complexity of a user's trajectory is measured using the mobile trajectory entropy. Second, classification criteria are proposed based on different user behavior patterns, and all users are classified with machine learning algorithms. Finally, according to the characteristics of each user classification, the step threshold and the weighting coefficients of the weighted Markov prediction model are optimized, and mobility prediction is performed for each user classification. Our results show that the optimized weighting coefficients can improve the performance of the weighted Markov prediction model.

4.
Aging (Albany NY) ; 13(6): 8248-8275, 2021 Mar 03.
Artigo em Inglês | MEDLINE | ID: mdl-33686952

RESUMO

This study aimed to identify effective targets for carcinogenesis of primary myelofibrosis (PMF), as well as to screen ideal lead compounds with potential inhibition effect on Janus kinase 2 to contribute to the medication design and development. Gene expression profiles of GSE26049, GSE53482, GSE61629 were obtained from the Gene Expression Omnibus database. The differentially expressed genes were identified, and functional enrichment analyses such as Gene Ontology, protein-protein interaction network etc., were performed step by step. Subsequently, highly-precise computational techniques were conducted to identify potential inhibitors of JAK2. A series of structural biology methods including virtual screening, ADMET (absorption, distribution, metabolism, excretion, and toxicity) prediction, molecule docking, molecular dynamics simulation etc., were implemented to discover novel natural compounds. Results elucidated that PMF patients had abnormal LCN2, JAK2, MMP8, CAMP, DEFA4, LTF, MPO, HBD, STAT4, EBF1 mRNA expression compared to normal patients. Functional enrichment analysis revealed that these genes were mainly enriched in erythrocyte differentiation, neutrophil degranulation and killing cells of other organisms. Two novel natural compounds, ZINC000013513540 and ZINC000004099068 were found binding to JAK2 with favorable interaction energy together with high binding affinity. They were predicted with non-Ames mutagenicity, low-rodent carcinogenicity, less developmental toxicity potential as well as non-toxicity with liver. Molecular dynamics simulation demonstrated that these two complexes: ZINC000013513540-JAK2 and ZINC000004099068-JAK2 could exist stably under natural circumstances. In conclusion, this study revealed hub genes in the carcinogenesis of PMF. ZINC000013513540 and ZINC000004099068 were promising drugs in dealing with PMF. This study may also accelerate exploration of new drugs.

5.
Environ Sci Pollut Res Int ; 28(14): 17044-17067, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33655478

RESUMO

Electronic cigarettes (e-cigarettes), since they do not require tobacco combustion, have traditionally been considered less harmful than conventional cigarettes (c-cigarettes). In recent years, however, researchers have found many toxic compounds in the aerosols of e-cigarettes, and numerous studies have shown that e-cigarettes can adversely affect the human epigenome. In this review, we provide an update on recent findings regarding epigenetic outcomes of e-cigarette aerosols. Moreover, we discussed the effects of several typical e-cigarette ingredients (nicotine, tobacco-specific nitrosamines, volatile organic compounds, carbonyl compounds, and toxic metals) on DNA methylation, histone modifications, and noncoding RNA expression. These epigenetic effects could explain some of the diseases caused by e-cigarettes. It also reminds the public that like c-cigarettes, inhaling e-cigarette aerosols could also be accompanied with potential epigenotoxicity on the human body.


Assuntos
Sistemas Eletrônicos de Liberação de Nicotina , Nitrosaminas , Produtos do Tabaco , Aerossóis , Humanos , Nicotina
6.
Phytomedicine ; 84: 153521, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33667838

RESUMO

BACKGROUND: Baeckein E (BF-2) was isolated from the aerial parts of Baeckea frutescens L., which has a long history of use in traditional medicine in Southeast Asia to treat inflammatory disease. PURPOSE: BF-2 was identified to have inhibitory activity on nucleotide oligomerization domain (NOD)-like receptor protein-3 inflammasome (NLRP3) activation. This study aimed to investigate the related signaling cascade of BF-2 in both lipopolysaccharides (LPS)/ATP induced pyroptosis in J774A.1 macrophages and its application in a mouse model of gout induced by monosodium urate crystal (MSU). METHODS: The effect of BF-2 on NLRP3 inflammasome activation and gouty arthritis was studied in J774A.1 macrophages and male C57BL/6 mice. The J774A.1 macrophages were primed with LPS and stained by propidium iodide (PI) for cell pyroptosis detection. A gout mouse model was established by subcutaneous injection of MSU crystals into the hind paw of C57BL/6 mice. Mice were then randomly divided into different groups. The concentrations of IL-1ß and IL-18 in both J774A.1 macrophage and gout mouse model were analyzed by ELISA. The NLRP3 inflammasome related protein expression was detected by western blot analysis. The inhibitory effects of BF-2 on NLRP3 inflammasome assembly were analyzed by immunoprecipitation assay. The roles of BF-2 in mitochondrial damage were imaged by Mito Tracker Green and Mito Tracker Red probes. The inhibitory effects of BF-2 on ROS production were imaged by DCF (2',7'-dichlorofluorescein diacetate) probe. RESULTS: The results demonstrated BF-2 could significantly suppress the cell pyroptosis and IL-1ß secretion in macrophages. Furthermore, BF-2 significantly inhibited NLRP3 inflammasome activation and reduced ankle swelling in the gout mouse model. In detail, it alleviated mitochondrial damage mediated oxidative stress and inhibited the assembly of NLRP3 inflammasome by affecting the binding of pro-Caspase 1 and ASC. Moreover, BF-2 blocked NLRP3 activation by inhibiting the MAPK/NF-κB signaling pathways. CONCLUSIONS: Results demonstrated BF-2 inhibited NLRP3 inflammasome activation in both LPS primed macrophages and mouse model of gout through blocking MAPK/NF-κB signaling pathway and mitochondrial damage mediated oxidative stress. This study strongly suggests BF-2 could be a promising drug candidate against inflammatory diseases associated with NLRP3 inflammasome activation.

7.
J Proteomics ; 240: 104190, 2021 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-33766670

RESUMO

Chronic stressors represented risk factors for the etiology or exacerbation of several gastrointestinal diseases. The goal of the present study was to examine whether chronic restraint stress (CRS) could initiate and aggravate colonic inflammation, integrity damage and metabolic disturbance of rats. Firstly, increased inflammatory cytokines (interferon-γ (IFN-γ), tumor necrosis factor-α(TNF-α) and interleukin-10(IL-10)) and decreased tight junction (TJ) proteins (occludin and zonula occludins-1 (ZO-1)) in rat colon were observed. Secondly, untargeted metabolomics based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass (UPLC-Q-TOF/MS) revealed that TRP metabolism was the most prominently affected. Thirdly, quantification of TRP and its metabolites via prominence ultrafast liquid chromatography coupled with a QTRAP 5500 mass (UFLC-QTRAP-5500/MS) showed that TRP, kynurenine (KYN), kynurenic acid (KA) and 3-hydroxykynurenine (3-HK) were significantly increased. At the same time, 5-hydroxytryptamine (5-HT) was unchanged and 5-hydroxyindolacetic acid (5-HIAA) was significantly decreased in the colon of CRS rats. Besides, TRP metabolic enzyme changes were with the same trends as the corresponding metabolites. Thus, our data showed that CRS could initiate colonic inflammation, integrity damage and colonic metabolism disturbance, especially TRP-KYN metabolism pathway of rats, which may provide an experimental background for future research on stress-related gastrointestinal dysfunction. SIGNIFICANCE: Chronic exposure to psychological stress could induce metabolic imbalance of the body, and stressful life events were intimately correlated with frequent relapses in patients with intestinal disorders. The present study showed that chronic restraint stress (CRS) could initiate and aggravate colonic inflammation, integrity damage and metabolic disturbance, especially tryptophan-kynurenine metabolism of rats. Tryptophan-kynurenine pathway may be involved in the initiation and development of diseases induced by chronic stress. This research may shed light on future research on stress-related gastrointestinal dysfunction.

8.
Toxins (Basel) ; 13(2)2021 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-33671260

RESUMO

The objective of this study was to evaluate the efficacy of mycotoxin binders in reducing the adverse effects of co-occurring dietary aflatoxin B1 (AFB1), deoxynivalenol (DON) and ochratoxin A (OTA) on laying hens. Three hundred and sixty 26-week-old Roman laying hens were randomly allocated into four experimental groups with 10 replicates of nine birds each. The four groups received either a basal diet (BD; Control), a BD supplemented with 0.15 mg/kg AFB1 + 1.5 mg/kg DON + 0.12 mg/kg OTA (Toxins), a BD + Toxins with Toxo-HP binder (Toxins + HP), or a BD + Toxins with TOXO XL binder (Toxins + XL) for 12 weeks. Compared to the control, dietary supplementation of mycotoxins decreased (P < 0.10) total feed intake, total egg weight, and egg-laying rate, but increased feed/egg ratio by 2.5-6.1% and mortality during various experimental periods. These alterations induced by mycotoxins were alleviated by supplementation with both TOXO HP and XL binders (P < 0.10). Furthermore, dietary mycotoxins reduced (P < 0.05) eggshell strength by 12.3% and caused an accumulation of 249 µg/kg of DON in eggs at week 12, while dietary supplementation with TOXO HP or XL mitigated DON-induced changes on eggshell strength and prevented accumulation of DON in eggs (P < 0.05). Moreover, dietary mycotoxins increased relative liver weight, but decreased spleen and proventriculus relative weights by 11.6-22.4% (P < 0.05). Mycotoxin exposure also increased alanine aminotransferase activity and reduced immunoglobulin (Ig) A, IgM, and IgG concentrations in serum by 9.2-26.1% (P < 0.05). Additionally, mycotoxin exposure induced histopathological damage and reduced villus height, villus height/crypt depth, and crypt depth in duodenum, jejunum and (or) ileum (P < 0.05). Notably, most of these histological changes were mitigated by supplementation with both TOXO HP and XL (P < 0.05). In conclusion, the present study demonstrated that the mycotoxin binders TOXO HP and XL can help to mitigate the combined effects of AFB1, DON, and OTA on laying hen performance, egg quality, and health.

9.
Huan Jing Ke Xue ; 42(2): 941-951, 2021 Feb 08.
Artigo em Chinês | MEDLINE | ID: mdl-33742890

RESUMO

In order to study the characteristics and factors influencing Cd accumulation in surface soils and crops in karst areas, and to provide a theoretical basis for safe land use, 360 surface soil samples, 7 deep soil samples, and 85 rice samples were collected from central Qianjiang District, Chongqing. The samples and 73 corn samples (corresponding to root-zone soil samples), were analysed to determine the content of Cd, TFe2 O3, Mn, organic matter (Corg), Se, and pH. Based on geostatistical analyses, the spatial distribution and Cd enrichment of the surface soils were determined and a safety evaluation for the soil and crops was carried out. The results showed that the spatial distribution of Cd in the surface soil was uneven, with the surface layer showing significant enrichment. This pattern was controlled by the soil parent material and human activities. The enrichment of surface layer was mainly affected by iron manganese oxides and organic matter (Corg). Soil Cd was mainly found at 'non-polluted' and 'lightly polluted' levels, although some areas present strong ecological risks. The main contaminated area occurs in association with Permian strata, demonstrating a geological control on soil Cd pollution. Slight-to-severe Cd pollution was identified in bulk crops; the recommended daily consumption limit for rice is 0.87 kg·d-1 and corn is 1.53 kg·d-1. The bioavailability of Cd is affected by soil pH and Se content. Under acidic conditions, Cd bioavailability is high, and crops in areas with high soil Se are safer. It is recommended that crops with low Cd accumulation are planted in the Permian outcrop area of Shuitian Township, or alternatively, soil pH should be adjusted to control the risk of Cd pollution and ensure safe land use. In addition, planting crops in areas with high soil Se content is preferable.

10.
Med Sci Monit ; 27: e929389, 2021 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-33714972

RESUMO

BACKGROUND Accumulated evidence has suggested that hydrogen sulfide (H2S) has a role in bone formation and bone tissue regeneration. However, it is unknown whether the H2S content is associated with bone mineral density (BMD) in patients with osteopenia/osteoporosis. MATERIAL AND METHODS In the present study, we aimed to explore the changes of serum H2S in osteopenia and osteoporosis patients. We analyzed femur expression of cystathionine ß synthase (CBS), cystathionine γ lyase (CSE), and 3-mercaptopyruvate sulfurtransferase (3-MST), which are key enzymes for generating H2S. RESULTS Sixteen (16%) patients had osteopenia, 9 (9%) had osteoporosis, and 75 (75%) had normal BMD. In comparison with patients with normal BMD (controls), the serum levels of H2S were unexpectedly increased in patients with osteopenia and osteoporosis. This increase was much higher in patients with osteoporosis than in those with osteopenia. Serum H2S levels were negatively correlated with femoral BMD, but not lumbar BMD. Interestingly, the expression of CBS and CSE were downregulated in femur tissues in patients with osteoporosis, whereas the expression of 3-MST remained unchanged. Serum phosphorus levels, alkaline phosphatase, hemoglobin, and triglycerides were found to be closely associated with CBS and CSE scores in femur tissues. CONCLUSIONS Serum H2S levels and femur CBS and CSE expression may be involved in osteoporosis pathogenesis.

11.
Genome Biol Evol ; 2021 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-33713114

RESUMO

One of the central goals in molecular evolutionary biology is to determine the sources of variation in the rate of sequence evolution among proteins. Gene expression level is widely accepted as the primary determinant of protein evolutionary rate, because it scales with the extent of selective constraints imposed on a protein, leading to the well-known negative correlation between expression level and protein evolutionary rate (the E-R anticorrelation). Selective constraints have been hypothesized to entail the maintenance of protein function, the avoidance of cytotoxicity caused by protein misfolding or nonspecific protein-protein interactions, or both. However, empirical tests evaluating the relative importance of these hypotheses remain scarce, likely due to the non-trivial difficulties in distinguishing the effect of a deleterious mutation on a protein's function vs. its cytotoxicity. We realized that examining the sequence evolution of viral proteins could overcome this hurdle. It is because purifying selection against mutations in a viral protein that result in cytotoxicity per se is likely relaxed, while purifying selection against mutations that impair viral protein function persists. Multiple analyses of SARS-CoV-2 and nine other virus species revealed a complete absence of any E-R anticorrelation. As a control, the E-R anticorrelation does exist in human endogenous retroviruses where purifying selection against cytotoxicity is present. Taken together, these observations do not support the maintenance of protein function as the main constraint on protein sequence evolution in cellular organisms.

12.
Sci Rep ; 11(1): 5802, 2021 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-33707599

RESUMO

Philadelphia chromosome-like (Ph-like) acute lymphoblastic leukaemia (ALL), a high-risk subtype characterised by genomic alterations that activate cytokine receptor and kinase signalling, is associated with inferior outcomes in most childhood ALL clinical trials. Half of the patients with Ph-like ALL have kinase rearrangements or fusions. We examined the frequency and spectrum of these fusions using a retrospective cohort of 212 newly diagnosed patients with childhood B-cell ALL. Samples without known chromosomal alterations were subject to multiplex reverse transcription polymerase chain reaction to identify known Ph-like kinase fusions. Immunoglobulin heavy chain locus (IGH) capture and kinase capture were applied to samples without known kinase fusions. We detected known kinase fusions in five of 212 patients, comprising EBF1-PDGFRB, ETV6-ABL1, ZC3HAV1-ABL2, EPOR-IGH, and CNTRL-ABL1. Two patients with P2RY8-CRLF2 were identified. Patients with non-Ph kinase fusions had inferior 5-year event-free survival and overall survival compared with patients with other common genetic alterations. The prevalence of non-Ph kinase fusions in our Taiwanese cohort was lower than that reported in Caucasian populations. Future clinical trials with tyrosine kinase inhibitors may be indicated in Taiwan because of the inferior outcomes for B-cell ALL with kinase fusions.

13.
Trials ; 22(1): 218, 2021 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-33736696

RESUMO

BACKGROUND: Cytomegalovirus retinitis (CMVR) is an important opportunistic infection (OI) occurring mainly in patients with acquired immunodeficiency syndrome (AIDS) and has the potential to cause severe visual impairment and blindness among AIDS patients. Subsequent to the adoption and implementation of widespread antiretroviral therapy (ART), the prognosis of AIDS-associated CMVR has been substantially improved. Nevertheless, the equivocal clinical evidence as regards the optimal timing for ART initiation in patients with an established CMVR diagnosis is required. We therefore designed the present study in order to investigate the optimal timing for ART initiation in AIDS/CMVR patients. METHODS: This will be a prospective, randomized controlled trial to be performed at 17 hospitals in mainland China. A total of 300 participants with CMVR will be randomly assigned to an early ART initiation group (ART initiation within 2 weeks after anti-CMV therapy), or a deferred ART initiation group (initiation of ART more than 2 weeks after anti-CMV therapy) at a 1:1 ratio. All participants will receive 48 weeks of follow-up after anti-CMV therapy initiation. Our primary outcome will be the incidence of visual loss (to a visual acuity worse than 20/40 or 20/200) in the two groups during the 48-week follow-up period. Secondary outcomes will include changes in HIV virological suppression and serum CD4+ T-cell counts, the incidence of mortality, retinitis progression (movement of the peripheral border of a CMV lesion ≥ ½ disc diameter, or occurrence of a new CMV lesion), retinal detachment, immune recovery uveitis (IRU), and other OIs and adverse events between the two study groups during the 48 weeks of follow-up. DISCUSSION: The study aims to investigate the optimal timing for ART initiation in AIDS/CMVR patients. We hope to be able to extract robust clinical evidence for use in optimal AIDS/CMVR management should our trial be successful. TRIAL REGISTRATION: This research was registered as one of the twelve clinical trials under the name of a general project "A study for precision diagnosing and treatment strategies in difficult-to-treat AIDS cases and HIV-infected patients with highly fatal or highly disabling opportunistic infections", ChiCTR1900021195. Registered on 1 February 2019, http://www.chictr.org.cn/showproj.aspx?proj=35362 .

14.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 35(3): 295-302, 2021 Mar 15.
Artigo em Chinês | MEDLINE | ID: mdl-33719236

RESUMO

Objective: To explore the effects of concentrated growth factor (CGF) combined with mineralized collagen (MC) materials on the adhesion, proliferation, and differentiation of bone marrow mesenchymal stem cells (BMSCs) and their osteogenic effects in vivo, and to provide a theoretical basis for the combined application of CGF and MC materials in bone defect regeneration and repair. Methods: CGF was prepared from venous blood of healthy volunteers, and then CGF extracts (CGFe) were prepared. In vitro experiment: human BMSCs (hBMSCs) were divided into 4 groups. Groups A, B, and C were cultured with α-MEM medium [containing 10% fetal bovine serum (FBS) and 1% double antibody] containing 2%, 5%, and 10%CGFe, respectively; group D was cultured with α-MEM medium (containing 10%FBS and 1% double antibody) without CGFe. Scanning electron microscopy was used to observe the effect of CGFe on cell adhesion. Cell counting kit 8 (CCK-8) was used to detect the effect of CGFe on cell proliferation. After osteogenic induction, alkaline phosphatase (ALP) activity was detected and Western blot was performed to detect osteopontin (OPN) expression. In vivo experiment: Eighteen New Zealand big-eared rabbits were used to prepare circular bone defect models on the left and right mandibles, and implant CGF gel (prepared from autologous venous blood)+MC material (volume ratio 1∶1, experimental group) and simple MC material (control group), respectively. At 4, 8, and 12 weeks after operation, 6 rabbits were sacrificed respectively to obtain materials, and Micro-CT scanning was performed to observe the formation of new bone and material degradation in vivo. Results: In vitro experiments: Scanning electron microscopy showed that the cells of groups A, B, and C spread better on MC materials than group D, with more pseudopodia. CCK-8 method showed that different concentrations of CGFe could promote cell proliferation, and the absorbance ( A) value of cells cultured for 2, 3, 5, and 7 days was in the order of group C>group B>group A>group D, the differences were significant ( P<0.05). ALP activity test showed that its activity was proportional to the osteogenic induction time and CGFe concentration ( P<0.05). Western blot analysis of osteogenic induction culture for 14 days showed that the relative expression of OPN protein in groups A, B, and C was significantly higher than that in group D, and the higher the CGFe concentration, the higher the relative expression of OPN protein ( P<0.05). In vivo experiment: Micro-CT observation showed that the new bone formation and material degradation of the experimental group were better than those of the control group at 4, 8, and 12 weeks after operation. Quantitative detection showed that the volume of new bone volume, new bone volume fraction, trabeculae number, and trabecular thickness of the experimental group were significantly higher than those of the control group at each time point, the residual material volume, residual material volume fraction, and trabecular separation were significantly lower than those of the control group, all showing significant differences ( P<0.05). Conclusion: CGF can effectively promote the adhesion, proliferation, and osteogenic differentiation of BMSCs on MC materials, and 10%CGFe has the most significant effect. The combined application of CGF and MC material can significantly promote bone formation in vivo.


Assuntos
Células-Tronco Mesenquimais , Osteogênese , Animais , Células da Medula Óssea , Diferenciação Celular , Proliferação de Células , Células Cultivadas , Colágeno , Peptídeos e Proteínas de Sinalização Intercelular , Coelhos
15.
Clin Chim Acta ; 517: 9-14, 2021 Feb 16.
Artigo em Inglês | MEDLINE | ID: mdl-33607069

RESUMO

BACKGROUND: Nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) plays an important role in the formation of oxidative stress in brain tissues. We intended to investigate relationship between serum NOX4 concentrations and severity, delayed cerebral ischemia (DCI) plus prognosis after aneurysmal subarachnoid hemorrhage (aSAH). METHODS: Serum NOX4 concentrations were gauged in a total of 165 aSAH patients. The World Federation of Neurological Surgeons (WFNS) scale and modified Fisher grading scale were recorded for assessing hemorrhagic severity. Relations of serum NOX4 concentrations to DCI and 90-day poor outcome (Glasgow outcome scale score of 1-3) were determined using multivariate analysis. RESULTS: Serum NOX4 concentrations were substantially higher in patients with 90-day poor outcome or DCI than in other remainders. Serum NOX4 concentrations of patients were intimately correlated with WFNS scores and modified Fisher scores. Serum NOX4 appeared as an independent predictor for DCI and 90-day poor outcome after aSAH. Under ROC curve analysis, serum NOX4 concentrations possessed significantly high predictive capability for DCI and 90-day poor outcome following hemorrhagic stroke. CONCLUSIONS: Serum NOX4 concentrations, in close correlation with hemorrhagic severity, were independently associated with DCI and poor clinical outcome after hemorrhagic stroke, substantializing serum NOX4 as a promising prognostic biomarker for aSAH.

16.
Sci Total Environ ; 772: 145522, 2021 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-33571779

RESUMO

With the continuous development of industrialization, a growing number of refractory organic pollutants are released into the environment. These contaminants could cause serious risks to the human health and wildlife, therefore their degradation and mineralization is very critical and urgent. Recently sulfate radical-based advanced oxidation technology has been widely applied to organic pollutants treatment due to its high efficiency and eco-friendly nature. This review comprehensively summarizes different methods for persulfate (PS) and peroxymonosulfate (PMS) activation including ultraviolet light, ultrasonic, electrochemical, heat, radiation and alkali. The reactive oxygen species identification and mechanisms of PS/PMS activation by different approaches are discussed. In addition, this paper summarized the toxicity of degradation intermediates through bioassays and Ecological Structure Activity Relationships (ECOSAR) program prediction and the formation of toxic bromated disinfection byproducts (Br-DBPs) and carcinogenic bromate (BrO3-) in the presence of Br-. The detoxification and mineralization of target pollutants induced by different reactive oxygen species are also analyzed. Finally, perspectives of potential future research and applications on sulfate radical-based advanced oxidation technology in the treatment of organic pollutants are proposed.


Assuntos
Poluentes Ambientais , Poluentes Químicos da Água , Humanos , Oxirredução , Sulfatos/toxicidade , Poluentes Químicos da Água/análise
17.
Vision Res ; 182: 20-26, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33578075

RESUMO

In the present study, we explored the perceptual span of skilled Uighur readers during their natural reading of sentences. The Uighur script is based on Arabic letters and it runs horizontally from right to left, offering a test to understand the effect of text direction. We utilized the gaze contingent moving window paradigm, in which legible text was provided only within a window that moved in synchrony with readers' eyes while all other letters were masked. The size of the window was manipulated systematically to determine the smallest size that allowed readers to show normal reading behaviors. Comparisons of window conditions with the baseline condition showed that the Uighur readers reached asymptotic performance in reading speed and gaze duration when windows revealed at least five letters to the right and twelve letters to the left of the currently fixated one. The present study is the first to document the size of the perceptual span in a horizontally leftwards running script. Cross-script comparisons with prior findings suggest that the size of the perceptual span for a certain writing system is likely influenced by its reading direction and visual complexity.

18.
Anal Chem ; 93(7): 3502-3509, 2021 02 23.
Artigo em Inglês | MEDLINE | ID: mdl-33544570

RESUMO

Visualizing and modulating the mitophagy process is essential for understanding the role of mitophagy in cellular homeostasis, physiology, and pathology. To overcome the sensing limitation of available mitophagy probes to only lysosome fusion or degradation, a molecular logic gate probe showing multiple fluorescence responses to different mitophagy stages was proposed in this study to sense the oxidative stress-induced mitophagy via a dual-channel mode. This new fluorescent molecular logic gate probe, Mito-PN, was composed by integrating a peroxynitrite-responsive 1,8-naphthalimide with an acidity-activatable rhodamine spirolactam and possesses the mitochondria-targeting capability due to its triphenylphosphonium group. This probe is able to sense both the mitophagy initiation triggered by peroxynitrite and lysosome fusion at different fluorescence wavelengths. It can be rapidly activated by mitochondrial peroxynitrite to turn on the green fluorescence of naphthalimide, and subsequent lysosome/mitophagosome fusion activates the probe with protons to generate red fluorescence. Moreover, our preliminary results demonstrate that the fluorescence response of Mito-PN to peroxynitrite-induced mitophagy can be discriminated from the mitophagy stimulated by carbonyl cyanide m-chlorophenyl hydrazone, which further proves the specific mitophagy tracking ability of Mito-PN. Overall, this research offers a potentially powerful tool for studying the role played by peroxynitrite in mitophagy and provides a versatile strategy for monitoring oxidative stress-related pathological processes.

19.
Biomed Pharmacother ; 137: 111320, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33578232

RESUMO

In China, Baitouweng Tang (BTWT) is a commonly prescribed remedy for the treatment of ulcerative colitis (UC). Herein, the present study aims to assess the anti-colitis activity of BTWT and its underlying mechanisms in UC BALB/c mice. Induction of UC in BALB/c mice was carried out by adding 3.5% DSS in the drinking water of underlined mice. After UC induction, the mice were administrated with BTWT for 7 days. Clinical symptoms were assessed, followed by analyzing the bile acids (BAs) in serum, liver, colon, bile, and feces of UC mice through UPLC-MS/MS. The modified 16S rDNA high-throughput sequencing was carried out to examine the gut microbiota of feces. BTWT significantly improved the clinical symptoms such as and histological injury and colon shortening in UC induced mice. Furthermore, BTWT remarkably ameliorated colonic inflammatory response. After BTWT treatment, the increased concentrations of UDCA, HDCA, αMCA, ßMCA, CA, and GLCA in UC were decreased, and the levels of some BAs, especially CA, αMCA, and ßMCA were normalized. Moreover, the relative species abundance and gut microbiota diversity in the BTWT-exposed groups were found to be considerably elevated than those in the DSS-treated group. BTWT increased the relative abundance of Firmicutes, Proteobacteria, Actinobacteria, Tenericutes, and TM7, which were statistically lower in the fecal microbiota of UC mice. The relative abundance of Bacteroidetes was found to be elevated in the DSS group and normalized after BTWT treatment. BTWT increased the expression of FXR and TGR5 in the liver. BTWT administration improved DSS-induced mice signs by increasing the TGR5 and FXR expression levels. This result was achieved by the regulation of the BAs and gut microbiota.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...