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2.
FASEB J ; 2020 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-32562311

RESUMO

Enhancer of zeste homolog 2 (EZH2), a well-known methyltransferase, mediates histone H3 lysine 27 trimethylation (H3K27me3) and plays a vital role in ophthalmological disease. However, its role in corneal neovascularization (CoNV) remains unclear. In vitro and in vivo models were assessed in hypoxia-stimulated angiogenesis and in a mouse model of alkali burn-induced CoNV. Human umbilical vein endothelial cells (HUVECs) were cultured under hypoxic conditions and different reoxygenation times to identify the molecular mechanisms involved in this process. In this study, we found that EZH2 was positively related to corneal alkali burn-induced injury. Inhibition of EZH2 with 3-Deazaneplanocin A (DZNeP) alleviated corneal injury, including oxidative stress and neovascularization in vivo. Similarly, inhibition of EZH2 with either DZNeP or small interfering RNA (siRNA) exerted an inhibitory effect on hypoxia/reoxygenation (H/R)-induced oxidative stress and angiogenesis in HUVECs. Moreover, our study revealed that ablation of reactive oxygen species (ROS) with N-acetyl-cysteine suppressed angiogenesis in HUVECs exposed to H/R stimulation. Furthermore, Forkhead-box protein O3a (FoxO3a), which was positively associated with ROS production and angiogenesis, was elevated during H/R. This effect could be reversed through the suppression of the transcription activity of EZH2 with DZNeP or siRNA. In addition, the PI3K/Akt pathway, which is the upstream of FoxO3a, was activated in both DZNeP-treated mice and EZH2-inhibited HUVECs. Collectively, our results demonstrated that the inhibition of EZH2 alleviated corneal angiogenesis by inhibiting FoxO3a-dependent ROS production through the PI3K/Akt signaling pathway. These findings indicate that EZH2 may be a valuable therapeutic target for CoNV.

3.
Clin Exp Rheumatol ; 2020 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-32573423

RESUMO

OBJECTIVES: Although relapsing polychondritis (RP) is considered as an immune-mediated systemic disease, the levels of peripheral lymphocyte subpopulations are rarely studied in patients with RP. In this study, we focused on changes of peripheral CD4+T cell subsets in patients with RP. METHODS: Absolute numbers and percentages of CD4+T cell subsets including helper T(Th)1, Th2, Th17 cells and regulatory T (Treg) cells in peripheral blood (PB) from 19 RP patients, healthy controls and RA patients respectively were assessed by flow cytometry combined a microbead-based single-platform method. We compared the CD4+T cell levels in all RP patients and healthy controls. In addition, we analysed the difference of the absolute number and percentage of Treg cells between RP and RA patients. RESULTS: Compared with healthy controls, all RP patients had significantly both lower absolute number and proportion of Treg cells (absolute number, 45.10/µl vs. 22.48/µl, p<0.001; proportion, 5.19% vs. 3.78%, p<0.001) no matter whether they had received treatment or not. Similarly, the absolute number of Th2 cells in all RP patients was decreased (10.19/µl vs. 7.44/µl, p=0.030). However, there were no significant differences in percentages and absolute numbers of Th1 and Th17 cells between RP patients and healthy controls. The above results led to increased ratios of Th1/Treg (3.68 vs. 2.06, p=0.020), Th2/Treg (0.29 vs. 0.21, p=0.037) and Th17/Treg (0.25 vs. 0.14, p<0.001) in RP patients, and untreated RP patients were mainly characterised by the imbalance of Th17/Treg (0.25 vs. 0.14, p<0.01). There was no significant difference in Treg cells between RP and RA patients (p>0.05). CONCLUSIONS: Our data suggest that the reduction of Treg cells and its imbalance with Th cells play an important role in the pathogenesis of RP.

4.
Exp Dermatol ; 2020 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-32580253

RESUMO

Reactive oxygen species (ROS) have already been demonstrated to impede the migratory ability in non-melanocytic cell lines by depleting mitochondrial ATP production. Therefore, understanding the mitochondrial metabolic response to migration in the presence of ROS should be a key to understanding repigmentation in vitiligo. This study aimed to investigate the energy mechanism associated with the ROS-mediated attenuation of melanocyte migration. After melanocytes were pretreated with H2 O2 , their ATP production, migratory ability, ultrastructural changes and Mitochondrial Permeability Potential were analyzed. The results showed that, in parallel with the decreased ATP production, the migratory ability of melanocytes was significantly inhibited by oxidative stress. Supplementation with exogenous ATP reversed the suppressed ATP-dependent migration of melanocytes. Melanocytes were then stressed with H2 O2 and Agilent Whole Human Genome microarray analysis identified 763 up-regulated mRNAs and 1117 down-regulated mRNAs. Among them, 11 of the encoded proteins were involved in mitochondrial ATP production and their expression levels were verified. The decreased expression of NADH dehydrogenase 2(ND2) , cytochrome c oxidase 1(COX1) and cytochrome c oxidase 2(COX3) was shown to be involved in the depletion of mitochondrial ATP production, which was coupled with the impaired migratory potential. These results indicate that the migration of melanocytes relies heavily on an inexhaustible supply of ATP from mitochondria.

5.
Food Chem ; 331: 127293, 2020 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-32554311

RESUMO

Wild rice (Zizania spp.), an important aquatic cereal grain in North America and East Asia, has attracted interest worldwide because of its antioxidant activities and health-promoting effects. Wild rice is high in protein, minerals, and vitamins but is low in fat. The phytochemical content (phytosterols, γ-oryzanol, γ-aminobutyric acid, phenolic acids, and flavonoids) of wild rice warrants its development as a functional food. Phenolic acids, flavonoids, and other phytochemicals from Zizania plants have pronounced antioxidant properties, which are associated with prevention of chronic diseases. The health-promoting effects of Zizania plants include alleviation of insulin resistance and lipotoxicity, atherosclerosis prevention, and anti-inflammatory, anti-allergic, anti-hypertensive, and immunomodulatory effects. Here, we provide an overview of Zizania research up to April 2020, focusing on the nutritional constituents, phytochemicals, antioxidant activities, and health-promoting effects of Zizania plants. This review has important implications for further investigations and applications of Zizania plants in medicine and as functional foods.

6.
Ann Noninvasive Electrocardiol ; : e12773, 2020 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-32564491

RESUMO

BACKGROUND: Atrial fibrillation (AF) is known to be the most common arrhythmia, and the successful rate of long-term ablation can vary comparatively. Therefore, a clinical scoring system to predict rhythm outcome remains a critical unmet need. The electrocardiographic (ECG) risk score which is named Morphology-Voltage-P-wave duration (MVP) score was reported to be useful for predicting new-onset AF. The goal of the current study was to investigate whether the MVP score was a useful scheme in the prediction of rhythm outcome following pulmonary vein isolation (PVI) in paroxysmal atrial fibrillation (PAF). METHODS: We retrospectively analyzed baseline characteristics, risk scores, and rates of AF recurrence 12 months postablation in the medical records of 207 consecutive patients with PAF undergoing PVI in General Hospital of Ningxia medical University from 2010 to 2018. RESULTS: Two hundred and seven patients (71 females, median age 58.7 years) with symptomatic PAF underwent PVI. From the cohort, 32.3% (67) had a recurrence of AF within 1 year of the PVI. The area of the MVP score under the curve in the receiver operating characteristics (ROC) analysis was 0.789 (95% CI 0.730-0.840, p < .001). A score cut-off value of >3 showed the best predictive ability for AF recurrence within 1 year after PVI, with sensitivity (53.03%) and specificity (89.87%). CONCLUSIONS: The results of our study suggest that the easy-to-measure ECG MVP score can be used to predict recurrence of PAF after PVI.

7.
Ther Deliv ; 2020 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-32522140

RESUMO

Glaucoma is one of the leading causes of visual loss in industrialized world. Local lowering intra-ocular pressure drops are the first-line treatment for glaucoma. Penetrating and nonpenetrating surgery is considered when topical lowering intra-ocular pressure drops fail. Recently, the iStent® device has been introduced in the management of glaucoma nonresponding sufficiently to local treatment. In this review, we present the results of the trials concerning the efficacy and safety of iStent for the treatment of open angle glaucoma.

8.
Chem Soc Rev ; 49(12): 3764-3782, 2020 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-32459227

RESUMO

Heterogeneous catalysts are vital to unlock superior efficiency, atom economy, and environmental friendliness in chemical conversions, with the size and speciation of the contained metals often playing a decisive role in the activity, selectivity and stability. This tutorial review analyses the impact of these catalyst parameters on the valorisation of biomass through hydrogenation and hydrodeoxygenation, oxidation, reforming and acid-catalysed reactions, spanning a broad spectrum of substrates including sugars and platform compounds obtained from (hemi)cellulose and lignin derivatives. It outlines multiple examples of classical structure sensitivity on nanoparticle-based materials with significant implications for the product distribution. It also shows how the recently emphasised application of metals in the form of ultrasmall nanoparticles (<2 nm), clusters and single atoms, while fulfilling superior metal utilisation and robustness, opens the door to unprecedented electronic and geometric properties. The latter can lead to facilitated activation of reactants as well as boosted selectivity control and synergy between distinct active sites in multifunctional catalysts. Based on the analysis conducted, guidelines for the selection of metals for diverse applications are put forward in terms of chemical identity and structure, and aspects that should be explored in greater depth for further improving the exploitation of metals in this research field and beyond are highlighted.

9.
Mol Med Rep ; 22(1): 165-174, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32319638

RESUMO

Hyperglycemia contributes to the excessive proliferation and migration of vascular smooth muscle cells (VSMC), which are closely associated with atherosclerosis. MicroRNAs (miRNAs/miRs) constitute a novel class of gene regulators, which have important roles in various pathological conditions. The aim of the present study was to identify miRNAs involved in the high glucose (HG)­induced VSMC phenotype switch, and to investigate the underlying mechanism. miRNA sequencing and reverse transcription­quantitative PCR results indicated that inhibition of miR­125a expression increased the migration and proliferation of VSMCs following HG exposure, whereas the overexpression of miR­125a abrogated this effect. Furthermore, dual­luciferase reporter assay results identified that 3­hydroxy­3-methyglutaryl­coA reductase (HMGCR), one of the key enzymes in the mevalonate signaling pathway, is a target of miR­125a. Moreover, HMGCR knockdown, similarly to miR­125a overexpression, suppressed HG­induced VSMC proliferation and migration. These results were consistent with those from the miRNA target prediction programs. Using a rat model of streptozotocin­induced diabetes mellitus, it was demonstrated that miR­125a expression was gradually downregulated, and that the expressions of key enzymes in the mevalonate signaling pathway in the aortic media were dysregulated after several weeks. In addition, it was found that HG­induced excessive activation of the mevalonate signaling pathway in VSMCs was suppressed following transfection with a miR­125a mimic. Therefore, the present results suggest that decreased miR­125a expression contributed to HG­induced VSMC proliferation and migration via the upregulation of HMGCR expression. Thus, miR­125a­mediated regulation of the mevalonate signaling pathway may be associated with atherosclerosis.

10.
Chin J Dent Res ; 23(1): 11-25, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32232225

RESUMO

Salivary adenoid cystic carcinoma (ACC) and pleomorphic adenoma (PA) are the most common types of salivary gland tumours; the former is malignant and the latter is benign but with features of a border tumour. Proteoglycans (PGs) produced by neoplastic myoepithelial cells are ubiquitous in both types of tumours. However, normal myoepithelial cells of salivary glands do not have the ability to secrete PGs. When the synthesis of PGs is blocked, the pulmonary metastasis and perineural growth of salivary ACC as well as the implanting growth of salivary PA are inhibited, highlighting the important functions of PGs in the tumourigenesis and development of these two tumours. In this review, we summarise literature from the past 40 years, including more recent findings from our laboratory, to clarify the pivotal roles of PGs produced by neoplastic myoepithelial cells in both the histogenesis and biological behaviours of ACC and PA.


Assuntos
Adenoma Pleomorfo , Carcinoma Adenoide Cístico , Carcinogênese , Humanos , Proteoglicanas , Glândulas Salivares
11.
Food Chem ; 318: 126483, 2020 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-32126468

RESUMO

In this study, the antioxidant activity of germinating Chinese wild rice was found to decline initially, after which it increased. The largest difference in antioxidant activity was observed between the 36-h (G36) and the 120-h germination (G120) stage. We further assessed the dynamic changes in metabolites, phenolic acids, flavonoids, and phenolic biosynthetic genes in germinating Chinese wild rice. Ultra-high performance liquid chromatography-triple quadrupole mass spectrometry revealed that 315 metabolites were up-regulated and 28 were down-regulated between G36 and G120. Levels of p-hydroxybenzoic acid, p-hydroxybenzaldehyde, vanillin, p-coumaric acid, ferulic acid, and epigallocatechin increased significantly during germination. Gene expression of four phenylalanine ammonia-lyases, one 4-coumarate-CoA ligase, one cinnamoyl-CoA reductase, two cinnamyl alcohol dehydrogenases, one chalcone synthase, and one chalcone isomerase was significantly higher at G120 than at G36 and promoted phenolics accumulation. This study elucidated the biochemical mechanisms involved in antioxidant activity and phenolic profile changes during Chinese wild rice germination.

12.
Artigo em Inglês | MEDLINE | ID: mdl-32180390

RESUMO

Solid oxide fuel cells (SOFCs) offer opportunities for the application as both power sources and chemical reactors. Yet, it remains a grand challenge to simultaneously achieve high efficiency of transforming higher hydrocarbons to value-added products and of generating electricity. To address it, we here present an ingenious approach of nanoengineering the triple-phase boundary of an SOFC anode, featuring abundant Co7W6@WOx core-shell nanoparticles dispersed on the surface of black La0.4Sr0.6TiO3. We also developed a cofeeding strategy, which is centered on concurrently feeding the SOFC anode with H2 and chemical feedstock. Such combined optimizations enable effective (electro)catalytic dehydrogenation of n-butane to butenes and 1,3-butadiene. The C4 alkene yield is higher than 50% while the peak power density of the SOFC reached 212 mW/cm2 at 650 °C. In addition, coke formation is largely suppressed and little CO/CO2 is produced in this process. While this work shows new possibility of chemical-electricity coupling in SOFCs, it might also open bona fide avenues toward the electrocatalytic synthesis of chemicals at higher temperatures.

13.
Cells ; 9(3)2020 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-32155989

RESUMO

Due to the complicated pathogenesis of Alzheimer's disease (AD), the development of multitargeted agents to simultaneously interfere with multiple pathological processes of AD is a potential choice. Glycogen synthase kinase-3ß (GSK-3ß) plays a vital role in the AD pathological process. In this study, we discovered a novel 1H-pyrrolo[2,3-b]pyridine derivative B10 as a GSK-3ß inhibitor that features with a quinolin-8-ol moiety to target the metal dyshomeostasis of AD. B10 potently inhibited GSK-3ß with an IC50 of 66 ± 2.5 nM. At the concentration of 20 µM, B10 increased ß-catenin abundance (ß-catenin/GAPDH: 0.83 ± 0.086 vs. 0.30 ± 0.016), phosphorylated GSK-3ß at Ser9 (p-GSK-3ß/GAPDH: 0.53 ± 0.045 vs. 0.35 ± 0.012), and decreased the phosphorylated tau level (p-tau/GAPDH: 0.33 ± 0.065 vs. 0.83 ± 0.061) in SH-SY5Y cells. Unlike other GSK-3ß inhibitors, B10 had a direct effect on Aß by inhibiting Aß1-42 aggregation and promoting the Aß1-42 aggregate disassociation. It selectively chelated with Cu2+, Zn2+, Fe3+, and Al3+, and targeted AD metal dyshomeostasis. Moreover, B10 effectively increased the mRNA expression of the recognized neurogenesis markers, GAP43, N-myc, and MAP-2, and promoted the differentiated neuronal neurite outgrowth, possibly through the GSK-3ß and ß-catenin signal pathways. Therefore, B10 is a potent and unique GSK-3ß inhibitor that has a direct on Aß and serves as a multifunctional anti-AD agent for further investigations.

14.
Microb Pathog ; 143: 104107, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32120003

RESUMO

Ustilago esculenta, a smut fungus, can induce the formation of culm galls in Zizania latifolia, a vegetable consumed in many Asian countries. Specifically, the mycelia-teliospore (M-T) strain of U. esculenta induces the Jiaobai (JB) type of gall, while the teliospore (T) strain induces the Huijiao (HJ) type. The underlying molecular mechanism responsible for the formation of the two distinct types of gall remains unclear. Our results showed that most differentially expressed genes relevant to effector proteins were up-regulated in the T strain compared to those in the M-T strain during gall formation, and the expression of teliospore formation-related genes was higher in the T strain than the M-T strain. Melanin biosynthesis was also clearly induced in the T strain. The T strain exhibited stronger pathogenicity and greater teliospore production than the M-T strain. We evaluated the implications of the gene regulatory networks in the development of these two type of culm gall in Z. latifolia infected with U. esculenta and suggested potential targets for genetic manipulation to modify the gall type for this crop.

15.
Proc Natl Acad Sci U S A ; 117(14): 7719-7728, 2020 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-32213582

RESUMO

Chitin is the most abundant renewable nitrogenous material on earth and is accessible to humans in the form of crustacean shell waste. Such waste has been severely underutilized, resulting in both resource wastage and disposal issues. Upcycling chitin-containing waste into value-added products is an attractive solution. However, the direct conversion of crustacean shell waste-derived chitin into a wide spectrum of nitrogen-containing chemicals (NCCs) is challenging via conventional catalytic processes. To address this challenge, in this study, we developed an integrated biorefinery process to upgrade shell waste-derived chitin into two aromatic NCCs that currently cannot be synthesized from chitin via any chemical process (tyrosine and l-DOPA). The process involves a pretreatment of chitin-containing shell waste and an enzymatic/fermentative bioprocess using metabolically engineered Escherichia coli The pretreatment step achieved an almost 100% recovery and partial depolymerization of chitin from shrimp shell waste (SSW), thereby offering water-soluble chitin hydrolysates for the downstream microbial process under mild conditions. The engineered E. coli strains produced 0.91 g/L tyrosine or 0.41 g/L l-DOPA from 22.5 g/L unpurified SSW-derived chitin hydrolysates, demonstrating the feasibility of upcycling renewable chitin-containing waste into value-added NCCs via this integrated biorefinery, which bypassed the Haber-Bosch process in providing a nitrogen source.

16.
Eye (Lond) ; 2020 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-32099079

RESUMO

BACKGROUND: This study reports the long-term visual and treatment outcomes in a whole-population, orthoptic-delivered pre-school visual screening (PSVS) programme in Scotland and further examines their associations with socioeconomic backgrounds and home circumstances. METHODS: Retrospective case review was conducted on 430 children who failed PSVS. Outcome measures included best corrected visual acuity (BCVA), severity of amblyopia (mild, moderate and severe), binocular vision (BV) (normal, poor and none), ophthalmic diagnosis and treatment modalities. Parameters at discharge were compared to those at baseline and were measured against the Scottish index of multiple deprivation (SIMD) and Health plan indicator (HPI), which are indices of deprivation and status of home circumstances. RESULTS: The proportion of children with amblyopia reduced from 92.3% (373/404) at baseline to 29.1% (106/364) at discharge (p < 0.001). Eighty percent (291/364) had good BV at discharge compared to 29.2% (118/404) at baseline (p < 0.001). Children from more socioeconomically deprived areas (OR 2.19, 95% CI 1.01-4.30, p = 0.003) or adverse family backgrounds (OR 3.94, 95% CI 1.99-7.74, p = 0.002) were more likely to attend poorly and/or become lost to follow-up. Children from worse home circumstances were five times more likely to have residual amblyopia (OR 5.37, 95% CI 3.29-10.07, p < 0.001) and three times more likely to have poor/no BV (OR 3.41, 95% CI 2.49-4.66, p < 0.001) than those from better home circumstances. CONCLUSIONS: Orthoptic-delivered PSVS is successful at screening and managing amblyopia. Children from homes requiring social care input are less likely to attend and are more likely to have poorer visual outcomes.

17.
Carbohydr Polym ; 234: 115892, 2020 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-32070512

RESUMO

The limited reactivity of starch towards maleic anhydride (MA) affords maleate with a low degree of MA substitutions (CC and COOH groups). In this study, we investigated the relationship between the starch structure, controlled by its amylose (AM)/amylopectin (AP) ratio, and the DS of starch maleates using C4[mim]Cl as the recyclable media, and catalyst. The results indicated that starches with varying AM/AP ratio produced maleates with comparable CC groups (DSNMR = 0.06-0.07). Following dissolution, the high amylose (DStitration = 1.17, yield = 69.2 %) and regular starches (DStitration = 1.17; yield = 59.3 %) produced high DStitration maleates (COOH groups) at MA/AGU ratio of 12:1 (80 °C, 10 min). Comparatively, DStitration value of waxy starch maleates (DStitration = 0.88, yield = 59.3 %) was lower than AM-based starches, possibly due to the crosslinking tendency of AP branches consisting of carboxylic end-groups. Interestingly, DStitration value for EHCS (1.17) ranged between its bulk (DSNMR: 0.06) and surface distribution of MA (DSSXPS 1.7); therefore, we considered it reliable for future reference.

18.
Development ; 147(6)2020 Mar 16.
Artigo em Inglês | MEDLINE | ID: mdl-32098764

RESUMO

Neocortex development during embryonic stages requires the precise control of mRNA metabolism. Human antigen R (HuR) is a well-studied mRNA-binding protein that regulates mRNA metabolism, and it is highly expressed in the neocortex during developmental stages. Deletion of HuR does not impair neural progenitor cell proliferation or differentiation, but it disturbs the laminar structure of the neocortex. We report that HuR is expressed in postmitotic projection neurons during mouse brain development. Specifically, depletion of HuR in these neurons led to a mislocalization of CDP+ neurons in deeper layers of the cortex. Time-lapse microscopy showed that HuR was required for the promotion of cell motility in migrating neurons. PCR array identified profilin 1 (Pfn1) mRNA as a major binding partner of HuR in neurons. HuR positively mediated the stability of Pfn1 mRNA and influenced actin polymerization. Overexpression of Pfn1 successfully rescued the migration defects of HuR-deleted neurons. Our data reveal a post-transcriptional mechanism that maintains actin dynamics during neuronal migration.

19.
Int J Nanomedicine ; 15: 633-645, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32099356

RESUMO

Background: With the increased application of Silver nanoparticles (AgNP), its potential concerns to the health of human beings remain to be defined. This study aims to explore the harmful effects of AgNP on lung tissue in animals and to examine the mechanisms of protection achieved by sodium selenite. Methods: Sprague-Dawley(SD) rats were exposed to AgNP (200 µL,1mg/mL) through a single intratracheal instillation. Sodium selenite (0.2mg/kg) was i.p. injected. Malondialdehyde (MDA) and glutathione (GSH) were measured using a spectrophotometer. Histological outcomes and ultrastructural changes were assessed by hematoxylin and eosin (HE) staining and electronic microscopy. Caspases and mitochondrial fission and fusion markers were measured by Western blotting. Results: The histopathologic findings showed that AgNP significantly increased the thickness of alveolar septa, accumulation of macrophage, and the formation of pulmonary bullae and pulmonary consolidation. Ultrastructural studies showed localization of AgNP inside the mitochondria, hyperplasia and vacuolation of type I and type II alveolar cells, lysis of osmiophilic lamellar bodies, and swollen of the mitochondria. AgNP elevated MDA and reduced GSH levels. AgNP activated caspases-3, increased mitochondrial fission markers Dynamin-related protein 1 (Drp1) and phospho-Drp1(p-Drp1), and decreased fusion proteins optic atrophy 1 (Opa1) and mitofusins 2 (Mfn2). Treatment with sodium selenite for 7 days corrected the AgNP-caused alterations in morphological, ultrastructural, oxidative stress, caspase-3 activation and mitochondrial dynamic imbalance. Conclusion: We conclude that the exposure of AgNP causes lung tissue damage by enhances oxidative stress, activates caspases-3, and triggers mitochondrial dynamic imbalance towards fission. Sodium selenite effectively detoxifies the AgNP-induced damage to the lung tissue by preventing the above alterations.


Assuntos
Pulmão/efeitos dos fármacos , Nanopartículas Metálicas/efeitos adversos , Dinâmica Mitocondrial/efeitos dos fármacos , Prata/efeitos adversos , Selenito de Sódio/farmacologia , Animais , Caspase 3/metabolismo , Morte Celular/efeitos dos fármacos , Dinaminas/metabolismo , GTP Fosfo-Hidrolases/metabolismo , Glutationa/metabolismo , Pulmão/metabolismo , Pulmão/patologia , Masculino , Malondialdeído/metabolismo , Nanopartículas Metálicas/química , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Mitocôndrias/ultraestrutura , Proteínas Mitocondriais/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Substâncias Protetoras/farmacologia , Ratos Sprague-Dawley , Prata/química
20.
Chem Commun (Camb) ; 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31909400

RESUMO

Phosphate functionalized carbon nanomaterials have attracted significant attention because of their potential applications in energy storage applications. Herein we report a facile one-pot method to prepare water dispersible phosphate functionalized reduced graphene oxide and demonstrate the potential of the novel materials for energy storage applications. The synthesis method shows promise to promote a wider adoption of reduced graphene oxide for high performance applications.

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