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1.
Artigo em Inglês | MEDLINE | ID: mdl-31670436

RESUMO

A novel surfactant, 6-((1 R ,4a S )-7-isopropyl-1,4a-dimethyl-1,2,3,4,4a,9,10,10a-octahydro-phenanthrene-1-carboxamido)- N , N - dimethylhexan-1-amine oxide (abbreviated as R-6-AO) derived from dehydroabietic acid has been synthesized which behaves as a highly efficient low molecular weight hydrogelator with an extremely low critical gelation concentration (CGC) of 0.18 wt.% (4 mM). As a typical surfactant, R-6-AO not only stabilizes oil-in-water (O/W) emulsions at concentrations above its critical micelle concentration (cmc) of 0.6 mM, but also forms gel emulsions at concentrations beyond the CGC with the oil volume fraction freely adjustable between 2% and 95%. Cryo-TEM images reveal that R-6-AO molecules self-assemble into left-handed helical fibers with cross-sectional diameters of about 10 nm in pure water which can be turned to very stable hydrogels at concentrations above the CGC. Compared with traditional high internal phase emulsions, the gel emulsions stabilized by R-6-AO can be prepared with different oils such as n-dodecane, n-decane, n-octane, soybean oil, olive oil and tricaprylin, due to the tricyclic diterpene hydrophobic structure in their molecules which enable them to adopt a unique arrangement in the fibers. The intriguing properties of gel emulsions stabilized by R-6-AO are believed to be significant and helpful for the preparation of new soft materials and other applications.

2.
Artigo em Inglês | MEDLINE | ID: mdl-31694379

RESUMO

Ternary non-fullerene all-small-molecule organic solar cells (NFSM-OSCs) were developed by incorporating a non-fullerene acceptor (IDIC) and two structurally similar small molecular donors (SM and SM-Cl), where SM-Cl is a novel small molecular donor derived from the reported molecular donor SM. When doping 10% SM-Cl in SM:IDIC binary system, the power conversion efficiency (PCE) of ternary solar cell was dramatically increased from 9.39% to 10.29%. Characterization studies indicated that the two donors tend to form an alloy state, which effectively down-shifted the HOMO energy level of the donor, and thus promoting a higher open-circuit voltage. Interestingly, incorporating a third component (SM-Cl) with lower crystallinity was proven to facilitate the demixing between donors and acceptors, which was in contrary to the traditional findings of enhanced phase separation through incorporation of highly crystalline molecule. Although the morphological modulation had always been a bottleneck issue in NFSM-OSCs, the findings in this work indicated that the modulation on crystallinity deviation between donors and acceptors could be an effective method to further improve the performance of NFSM-OSCs, providing a new perspective on NFSM-OSCs.

3.
Oncogene ; 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31641208

RESUMO

Genome-wide association studies (GWASs) implicate 16q22.1 locus in risk for colorectal cancer (CRC). However, the underlying oncogenic mechanisms remain unknown. Here, through comprehensive filtration, we prioritized rs7198799, a common SNP in the second intron of the CDH1, as the putative causal variant. In addition, we found an association of CRC-risk allele C of rs7198799 with elevated transcript level of biological plausible candidate gene ZFP90 via expression quantitative trait loci analysis. Mechanistically, causal variant rs7198799 resides in an enhancer element and remotely regulate ZFP90 expression by targeting the transcription factor NFATC2. Remarkably, CRISPR/Cas9-guided single-nucleotide editing demonstrated the direct effect of rs7198799 on ZFP90 expression and CRC cellular malignant phenotype. Furthermore, ZFP90 affects several oncogenic pathways, including BMP4, and promotes carcinogenesis in patients and in animal models with ZFP90 specific genetic manipulation. Taken together, these findings reveal a risk SNP-mediated long-range regulation on the NFATC2-ZFP90-BMP4 pathway underlying the initiation of CRC.

4.
Artigo em Inglês | MEDLINE | ID: mdl-31653053

RESUMO

PURPOSE: Very few studies have examined the influential factors of survivors' feelings of happiness in the context of nuclear accidents. This paper aims to fill this gap with reference to the recovery process in Fukushima City following the 2011 Tohoku Earthquake Tsunami in Japan. METHODS: Open access data were sourced from the 2015 Social Survey on Living and Disaster Recovery (SSLDR) (N = 1439) of Fukushima citizens. Pearson's Chi-square Test and the t-test were employed to examine gender differences with regard to happiness and exploratory variables. Following this, a multiple linear regression analysis was conducted to investigate the determinants of happiness. RESULTS: The results showed that, compared to females, male respondents were unhappier and reported more property loss and less neighborhood connectedness. Individuals' mental and physical health and neighborhood connectedness were found to be significantly correlated with their happiness. However, the disaster-related variables of people's evaluation of recovery achievement, concerns around the health impacts of radiation, property loss in the disaster, and experiences of casualty, had no effects on happiness. CONCLUSION: These findings indicate that policies and countermeasures dealing with disaster recovery over the long term should continuously focus on health issues and social relationships.

5.
World J Microbiol Biotechnol ; 35(11): 165, 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31641866

RESUMO

Genome shuffling for improving the activity of alkaline pectinase in Bacillus subtilis FS105 and its molecular mechanism were investigated. The fused strain B. subtilis FS105 with the highest activity of alkaline pectinase was obtained after two rounds of genome shuffling. The activity of alkaline pectinase in B. subtilis FS105 was 499 U/ml, which was improved by 1.6 times compared to that in original strain. To elucidate its molecular mechanism, rpsL gene sequences from original and fused strains were cloned and aligned, and the space structure of their coding proteins were also analyzed and compared. The alignment of the rpsL gene sequences indicated that three bases G, G and C were respectively replaced by A, A and G in the positions 52, 408 and 409 after genome shuffling. This resulted in the substitution of two amino acid residues in ribosomal protein S12: D18N and P137A, and therefore improving the biosynthesis of alkaline pectinase. This study lays a foundation for improving the activity of alkaline pectinase by genome shuffling and understanding its molecular mechanism.

6.
ACS Appl Mater Interfaces ; 11(45): 42447-42454, 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31625386

RESUMO

Ag nanowires (NWs)/PEDOT:PSS composite was prepared by a facile solution-processing method and employed as anode interface in nonfullerene organic solar cells (OSCs). In the presence of a Ag NWs (5%, v/v%)/PEDOT:PSS interfacial layer, a high-power conversion efficiency up to 13.53% was achieved based on a PBDB-T-2Cl:IT-4F photoactive layer system, much higher than the efficiency of the controlled counterpart device with pristine PEDOT:PSS as anode modifier. Simultaneous enhancements in short-circuit current and fill factor were observed, in comparison to the case of the pristine PEDOT:PSS interface, due to the improved electrical conductivity of Ag NWs/PEDOT:PSS composites accompanied by the increased work function for a better matching with the indium tin oxide counter electrode, which facilitated increased charge transfer and reduced charge recombination at the anode/photoactive interface for improved device performance. The results clearly revealed that the Ag NWs/PEDOT:PSS composite interface is beneficial to improve the charge extraction and favor the realization of highly efficient nonfullerene OSCs.

7.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 50(3): 334-338, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31631599

RESUMO

Objective: To study the mechanism of renal injury in Lepr db/ db mice with the leptin receptor homozygous deficiency. Methods: Ten male of 28-week-old Lepr db/+ mice with leptin receptor heterozygous deficiency were selected as control group and ten male Lepr db/ db mice with leptin receptor homozygous deficiency were used in this study. After fasting for 8 hours, the body mass, fasting blood glucose (FBG) and glycosylated hemoglobulin (HbA1c) of the mice were measured. Blood of the mice was obtained from femoral artery before euthanasia. Serum creatinine (CRE), blood urea nitrogen (BUN), superoxide dismutase (SOD), glutathione (GSH) and malonaldehyde (MDA) were detected by corresponding kits, and serum interleukin-1ß (IL-1ß), monocyte chemotactic protein-1 (MCP-1) and tumor necrosis factor-α (TNF-α) were measured using enzyme-linked immunosorbent assay (ELISA) method. The kidney was taken for pathological observation. The expression levels of nuclear factor E2-related factor 2 (Nrf2) and nuclear factor kappa B (NF-κB) in renal were analyzed by Western blot. The mitochondria of renal was isolated by the corresponding kit. Meanwhile, the expression level of lipoic acid synthase (LIAS) in renal mitochondria was measured by Western blot. Results: The body mass, FPG, HbA1c, CRE and BUN levels of the Lepr db/ db mice were significantly increased in comparison with the Lepr db/+ mice ( P<0.05). Compared with the Lepr db/+ mice, the Lepr db/ db mice renal exhibited glomerular hypertrophy, thickened basement membrane and capillary wall, the mesangial matrix expansion and mesangial cell hyperplasia. Compared with the Lepr db/+ mice, the serum level of GSH in the Lepr db/ db mice was decreased significantly ( P<0.05). The levels of MDA and concentrations of MCP-1, IL-1ß and TNF-α in serum of the Lepr db/ db mice were higher than those of the Lepr db/+ mice ( P<0.05). Compared with the Lepr db/+ mice, the expression of LIAS and Nrf2 protein in the Lepr db/ db mice renal were decreased ( P<0.05), while the expression of NF-κB protein was increased ( P<0.05). Conclusion: LIAS, Nrf2 and NF-κB might play significant roles through regulation of oxidative stress and inflammation in the renal injury of Lepr db/ db mice.


Assuntos
Rim/patologia , Fator 2 Relacionado a NF-E2/metabolismo , NF-kappa B/metabolismo , Receptores para Leptina/genética , Sulfurtransferases/metabolismo , Animais , Masculino , Camundongos , Camundongos Knockout , Estresse Oxidativo
8.
Biosens Bioelectron ; 146: 111743, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31586760

RESUMO

Metal-organic frameworks (MOFs) as molecular crystalline materials have been extensively applied in various fields such as catalysis, separation, and biomedical engineering. However, the applications of MOFs materials are limited in electrochemical biosensing due to the poor conductivity, less selectivity, and lack of modification sites. By incorporating the functionalized nanoparticles into MOF structures, MOF-based composites are endowed with high electronic conductivity and strong catalytic activity, which process the advantages over single-component MOFs. With a particular focus on the electrochemical applications of MOF composites, this review summarizes the comprehensive guidelines on design of electroactive MOF composites: dopant modification of electroactive ligands, in situ synthesis of nanoparticle@MOF composites and post-modification of MOF structure. The illustrative examples of electroactive MOF composites in the last five years are highlighted in electrochemical, electrochemiluminescent, and photoelectrochemical biosensing. The prospects and challenges for future work are also included. Understanding the structure-function relationship of electroactive MOF composites benefits the design of next-generation electrochemical biosensors.

9.
BMC Cancer ; 19(1): 877, 2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31488093

RESUMO

BACKGROUND: Breast cancer is the most common malignancy in women, and neoadjuvant chemotherapy has been recommended to the patients with locally advanced breast cancer as the initial treatments. Long non-coding RNA (lncRNA) MEG3, an identified tumor suppressor, has been implicated in the development of various cancers. However, there is no data to evaluate the effect of MEG3 polymorphisms on neoadjuvant treatment in the breast cancer. METHODS: Genotyping was performed using Nanodispenser Spectro CHIP chip spotting and Mass ARRAY Compact System. Univariate and multivariate logistic regression analyses were used to analyze the associations between the MEG3 polymorphisms and the pathological complete response (pCR). The disease-free survival (DFS) was estimated by the Kaplan-Meier method, and multivariate Cox proportional hazards models were used to calculate the hazard ratios (HRs) with a 95% confidential interval (CI). RESULTS: A total of 144 patients with available pretreatment blood species were enrolled in the SHPD002 clinic trial of neoadjuvant chemotherapy for breast cancer. MEG3 rs10132552 were significantly associated with good response (Adjusted OR = 2.79, 95% CI 1.096-7.103, p = 0.031) in dominant model. Median follow-up time was 20 months. In multiple regression analysis, rs10132552 TC + CC (adjusted HR = 0.127, 95% CI 0.22-0.728, p = 0.02) and rs941576 AG + GG (adjusted HR = 0.183, 95% CI 0.041-0.807, p = 0.025) were significantly associated with good DFS. MEG3 rs7158663 (OR = 0.377, 95% CI 0.155-0.917, p = 0.032) were associated with a low risk of hemoglobin decrease in dominant models. CONCLUSIONS: LncRNA MEG3 polymorphisms were associated with the chemotherapy response and toxicity of paclitaxel and cisplatin. The result indicates that MEG3 polymorphisms can be considered as the predictive and prognostic markers for the breast cancer patients. TRIAL REGISTRATION: Retrospectively registered (ClinicalTrials. Gov identifier: NCT02221999 ); date of registration: Aug 20th, 2014.

10.
DNA Cell Biol ; 38(10): 1078-1087, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31524500

RESUMO

The globally distributed American cockroach (Periplaneta americana) is considered a pest, but it has been widely used in traditional Chinese medicine. In the past, the American cockroach's genome and transcriptomes were sequenced, but the differential expression transcripts between developmental stages were unavailable. We performed the de novo assembly and analysis of American cockroach transcriptomes from four developmental stages. Approximately 200 million high-quality paired-end reads were generated by using Illumina Hiseq 2000 sequencer. The assembly produced 291,250 transcripts with an average length of 714 bp. In addition, 38,052 microsatellites and 11,060,020 transposable elements were identified. Based on sequence homology, 53,262 transcripts were annotated. After calculating the expression levels of all the transcripts, we found that 13 transcripts were highly expressed in all the samples and at least two, p10 and actin-related protein 1, played important roles during development. A total of 7954 differentially expressed transcripts (DETs) were identified. The adult had the largest number of DETs when compared to other samples (4818), while the 3rd and 8th larva had the least number of DETs (1332). We performed gene enrichment analysis with the DETs, and some interesting results were detected in the different groups. For example, chitin is the major component of the insect exoskeleton, and the chitin-related genes in larvae and new molted samples had higher expression levels than in adults. In addition, the enrichment analysis detected many chitin-related pathways. Our study performed the first large-scale comparative transcriptomics between the developmental stages of American cockroach, which could provide useful gene expression data for future studies.


Assuntos
Genoma de Inseto , Estágios do Ciclo de Vida/genética , Redes e Vias Metabólicas/genética , Periplaneta/genética , Transcriptoma , Animais , Elementos de DNA Transponíveis , Feminino , Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Ontologia Genética , Sequenciamento de Nucleotídeos em Larga Escala , Masculino , Repetições de Microssatélites , Anotação de Sequência Molecular , Periplaneta/classificação , Periplaneta/crescimento & desenvolvimento , Periplaneta/metabolismo , Filogenia
11.
Environ Sci Technol ; 53(21): 12668-12676, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31532191

RESUMO

Capacitive deionization (CDI) has received wide attention as an emerging water treatment technology because of its low energy consumption, low cost, and high efficiency. However, the conventional carbon electrode materials for CDI have low densities, which occupy large volumes and are disadvantageous for use in limited space (e.g., in household or on offshore platforms). In order to miniaturize the CDI device, it is quite urgent to develop high volumetric adsorption capacity (VAC) electrode materials. To overcome this issue, we rationally designed and originally developed high VAC MoS2-graphene hybrid electrodes for CDI. It is interesting that MoS2-graphene hybrid electrode has a much higher NaCl VAC of 14.3 mg/cm3 with a gravimetric adsorption capacity of 19.4 mg/g. It has been demonstrated that the adsorption capacity is significantly enhanced because of the rapid ion transport of MoS2 and high electrical conductivity of graphene. In situ Raman spectra and high-angle annular dark-field scanning transmission electron microscopy tests demonstrated a favorable Faradaic reaction, which was crucial to enhancing the NaCl VAC of the MoS2-graphene hybrid electrode. This work opens a new avenue for miniaturizing future CDI devices.

12.
Can J Microbiol ; 65(12): 913-921, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31491332

RESUMO

This study reports the use of reverse transcription - loop-mediated isothermal amplification (RT-LAMP) to detect Listeria monocytogenes in meat. The assay was designed to target the iap gene of L. monocytogenes, to which four primers, recognizing six distinct iap sites, were designed. We optimized the RT-LAMP conditions and established the following optimal systems: 60 min, 63 °C, 2.0 mmol/L MgSO4, 1.0 mol/L betaine, 2.0 mmol/L dNTPs, 320 U/mL Bst DNA polymerase, 0.4 µmol/L outer primers, and 0.8 µmol/L inner primers. The RT-LAMP amplification products were identified by a visible white Mg2P2O7 precipitate or electrophoresis on a 2% agarose gel. RT-LAMP has a sensitivity of 7.3 × 101 CFU/mL, which is 2-fold higher than that of LAMP. When commercially available raw meat samples (including beef, pork, mutton, and rabbit) were analyzed simultaneously with RT-LAMP and the Chinese National Standard GB 4789.30-2016, their abilities to detect L. monocytogenes were the same. Samples containing L. monocytogenes killed by 15 psi at 121 °C for 15 min were used to confirm the specificity of RT-LAMP for live microorganisms. Thus, we used RT-LAMP to efficiently detect L. monocytogenes in meat products.

13.
Adv Mater ; 31(39): e1902210, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31411359

RESUMO

Ternary heterojunction strategies appear to be an efficient approach to improve the efficiency of organic solar cells (OSCs) through harvesting more sunlight. Ternary OSCs are fabricated by employing wide bandgap polymer donor (PM6), narrow bandgap nonfullerene acceptor (Y6), and PC71 BM as the third component to tune the light absorption and morphologies of the blend films. A record power conversion efficiency (PCE) of 16.67% (certified as 16.0%) on rigid substrate is achieved in an optimized PM6:Y6:PC71 BM blend ratio of 1:1:0.2. The introduction of PC71 BM endows the blend with enhanced absorption in the range of 300-500 nm and optimises interpenetrating morphologies to promote photogenerated charge dissociation and extraction. More importantly, a PCE of 14.06% for flexible ITO-free ternary OSCs is obtained based on this ternary heterojunction system, which is the highest PCE reported for flexible state-of-the-art OSCs. A very promising ternary heterojunction strategy to develop highly efficient rigid and flexible OSCs is presented.

14.
J Pharmacol Exp Ther ; 371(2): 360-374, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31420528

RESUMO

Nonalcoholic steatohepatitis (NASH) is the progressive stage of nonalcoholic fatty liver disease that highly increases the risk of cirrhosis and liver cancer, and there are few therapeutic options available in the clinic. Withaferin A (WA), extracted from the ayurvedic medicine Withania somnifera, has a wide range of pharmacological activities; however, little is known about its effects on NASH. To explore the role of WA in treating NASH, two well defined NASH models were used, the methionine-choline-deficient diet and the 40 kcal% high-fat diet (HFD). In both NASH models, WA treatment or control vehicle was administered to evaluate its hepatoprotective effects. As assessed by biochemical and histologic analyses, WA prevented and therapeutically improved liver injury in both models, as revealed by lower serum aminotransaminases, hepatic steatosis, liver inflammation, and fibrosis. In the HFD-induced NASH model, both elevated serum ceramides and increased hepatic oxidative stress were decreased in the WA-treated group compared with the control vehicle-treated group. To further explore whether WA has an anti-NASH effect independent of its known action in leptin signaling associated with obesity, leptin signaling-deficient ob/ob mice maintained on an HFD were used to induce NASH. WA therapeutically reduced NASH in HFD-treated leptin-deficient ob/ob mice, thus demonstrating a leptin-independent hepatoprotective effect. This study revealed that WA treatment could be an option for NASH treatment.

15.
Nat Commun ; 10(1): 3499, 2019 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-31375671

RESUMO

Long non-coding RNAs (lncRNAs) contribute to colorectal cancer (CRC). However, the role of lncRNAs in CRC metabolism, especially glucose metabolism remains largely unknown. In this study, we identify a lncRNA, GLCC1, which is significantly upregulated under glucose starvation in CRC cells, supporting cell survival and proliferation by enhancing glycolysis. Mechanistically, GLCC1 stabilizes c-Myc transcriptional factor from ubiquitination by direct interaction with HSP90 chaperon and further specifies the transcriptional modification pattern on c-Myc target genes, such as LDHA, consequently reprogram glycolytic metabolism for CRC proliferation. Clinically, GLCC1 is associated with tumorigenesis, tumor size and predicts poor prognosis. Thus, GLCC1 is mechanistically, functionally, and clinically oncogenic in colorectal cancer. Targeting GLCC1 and its pathway may be meaningful for treating patients with colorectal cancer.

16.
Am J Dent ; 32(4): 187-190, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31436939

RESUMO

PURPOSE: To evaluate the shear bond strength between denture base and artificial teeth subjected to five different modifications on the ridge surface. METHODS: 30 acrylic central anterior teeth were randomly divided into five groups (n= 6). The ridge surface of these teeth were treated with different methods: (1) No treatment applied; (2) Monomer wetting; (3) Grinding; (4) Grinding followed by sandblasting; (5) Grinding followed by monomer wetting. After the ridge surface of the teeth were treated, they were packed with denture base resin. The shear bond strength between acrylic teeth and denture base resin was performed using a universal testing machine. The data was statistically analyzed using one-way ANOVA (P< 0.05). RESULTS: The monomer wetting group showed the highest shear bond strength values, and the grinding followed by sandblasting group was the lowest, both were statistically significant compared to each other. There were no statistical differences between the other groups. CLINICAL SIGNIFICANCE: Treating the surface of the denture ridge with a monomer provided the highest shear bond strength values, and the grinding followed by sandblasting group was the lowest, statistically significant compared to each other.


Assuntos
Resinas Acrílicas , Colagem Dentária , Polímeros , Análise do Estresse Dentário , Bases de Dentadura , Teste de Materiais , Distribuição Aleatória , Resistência ao Cisalhamento , Propriedades de Superfície
18.
Hereditas ; 156: 25, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31346329

RESUMO

Background: Alzheimer's disease (AD) is known to be caused by multiple factors, meanwhile the pathogenic mechanism and development of AD associate closely with genetic factors. Existing understanding of the molecular mechanisms underlying AD remains incomplete. Methods: Gene expression data (GSE48350) derived from post-modern brain was extracted from the Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) were derived from hippocampus and entorhinal cortex regions between AD patients and healthy controls and detected via Morpheus. Functional enrichment analyses, including Gene Ontology (GO) and pathway analyses of DEGs, were performed via Cytoscape and followed by the construction of protein-protein interaction (PPI) network. Hub proteins were screened using the criteria: nodes degree≥10 (for hippocampus tissues) and ≥ 8 (for entorhinal cortex tissues). Molecular Complex Detection (MCODE) was used to filtrate the important clusters. University of California Santa Cruz (UCSC) and the database of RNA-binding protein specificities (RBPDB) were employed to identify the RNA-binding proteins of the long non-coding RNA (lncRNA). Results: 251 & 74 genes were identified as DEGs, which consisted of 56 & 16 up-regulated genes and 195 & 58 down-regulated genes in hippocampus and entorhinal cortex, respectively. Biological analyses demonstrated that the biological processes and pathways related to memory, transmembrane transport, synaptic transmission, neuron survival, drug metabolism, ion homeostasis and signal transduction were enriched in these genes. 11 genes were identified as hub genes in hippocampus and entorhinal cortex, and 3 hub genes were identified as the novel candidates involved in the pathology of AD. Furthermore, 3 lncRNAs were filtrated, whose binding proteins were closely associated with AD. Conclusions: Through GO enrichment analyses, pathway analyses and PPI analyses, we showed a comprehensive interpretation of the pathogenesis of AD at a systematic biology level, and 3 novel candidate genes and 3 lncRNAs were identified as novel and potential candidates participating in the pathology of AD. The results of this study could supply integrated insights for understanding the pathogenic mechanism underlying AD and potential novel therapeutic targets.

19.
Food Chem ; 297: 124912, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31253263

RESUMO

An anti-idiotypic nanobody-phage display-mediated immuno-polymerase chain reaction (PD-IPCR) method was developed for simultaneous quantitative detection of total aflatoxins and zearalenone in cereals. Two phages, displaying the variable domain of the heavy chain anti-idiotypic nanobody that binds aflatoxin- or zearalenone-specific monoclonal antibody (1C11 or 2D3), were used as competitors for corresponding analytes. Specific DNA sequences encoding anti-idiotypic nanobodies were used to design the primers for PCR amplification. The results indicated that detection limits for total aflatoxins and zearalenone in a sample were 0.03 and 0.09 ng mL-1, respectively. Recoveries of spiked aflatoxins and zearalenone were 80-118% and 76.7-111%, respectively. Validation results were in good agreement with the gold-standard high-performance liquid chromatography method. This report is the first to describe PD-IPCR for simultaneous quantitative detection of total aflatoxins and zearalenone in cereals.


Assuntos
Aflatoxinas/análise , Anticorpos Anti-Idiotípicos/imunologia , Imunoensaio/métodos , Zearalenona/análise , Aflatoxinas/imunologia , Anticorpos Anti-Idiotípicos/genética , Cromatografia Líquida de Alta Pressão , Limite de Detecção , Oryza/química , Oryza/metabolismo , Biblioteca de Peptídeos , Reação em Cadeia da Polimerase em Tempo Real/métodos , Anticorpos de Domínio Único/genética , Anticorpos de Domínio Único/imunologia , Zea mays/química , Zea mays/metabolismo , Zearalenona/imunologia
20.
Prep Biochem Biotechnol ; 49(7): 718-726, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31050583

RESUMO

A propanol-tolerant neutral protease was purified and characterized from Bacillus sp. ZG20 in this study. This protease was purified to homogeneity with a specific activity of 26,655 U/mg. The recovery rate and purification fold of the protease were 13.7% and 31.5, respectively. The SDS-PAGE results showed that the molecular weight of the protease was about 29 kDa. The optimal temperature and pH of the protease were 45 °C and 7.0, respectively. The protease exhibited a good thermal- and pH stability, and was tolerant to 50% propanol. Mg2+, Zn2+, K+, Na+ and Tween-80 could improve its activity. The calculated Km and Vmax values of the protease towards α-casein were 12.74 mg/mL and 28.57 µg/(min mL), respectively. This study lays a good foundation for the future use of the neutral protease from Bacillus sp. ZG20.


Assuntos
1-Propanol/metabolismo , Bacillus/enzimologia , Peptídeo Hidrolases/metabolismo , Bacillus/química , Bacillus/metabolismo , Detergentes/metabolismo , Eletroforese em Gel de Poliacrilamida , Estabilidade Enzimática , Peróxido de Hidrogênio/metabolismo , Concentração de Íons de Hidrogênio , Íons/metabolismo , Metais/metabolismo , Peptídeo Hidrolases/química , Peptídeo Hidrolases/isolamento & purificação , Solventes/metabolismo , Especificidade por Substrato , Tensoativos/metabolismo , Temperatura Ambiente
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