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1.
Food Microbiol ; 101: 103891, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34579851

RESUMO

In recent years, more attention has been paid to the application of cold plasma (CP) in eliminating foodborne pathogenic bacteria. This work investigated CP effects on inactivation kinetics and cell envelopes of Listeria monocytogenes (L. monocytogenes) and Salmonella Enteritidis (S. Enteritidis). Bacterial suspensions were treated with dielectric barrier discharge atmospheric CP at 75 kV for different treatment time. Three regression models were tested for estimating inactivation kinetics. Reactive species generated in plasma, the appearance and integrity of bacterial cells, the activity and secondary structure of enzymes in the cell envelope, and molecular docking, were measured for evaluating the envelope damages. Results indicated that Log-linear model was suitable for L. monocytogenes and the Weibull model was suitable for S. Enteritidis. S. Enteritidis was more sensitive to short-lived reactive species (such as OH radicals) in plasma than L. monocytogenes, and the cell envelope of S. Enteritidis was more severely damaged (the increased membrane permeability and leakage of intracellular substances) after plasma treatment. Interestingly, compared with S. Enteritidis, the decrease in the activity of enzymes existing in the cell envelope of L. monocytogenes did not contribute significantly to the death of bacteria. Molecular docking further suggested that the decrease in the enzyme activity might be due to the modification of the enzyme, by the interaction between reactive species in plasma (H2O2) and amino acid residues of the enzyme through the hydrogen bond.

2.
Front Psychol ; 12: 704614, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34744866

RESUMO

Previous studies have found that promoting multiple identities can improve children's creative performance (divergent thinking). The present study employed a priming paradigm to design two experiments and investigate whether promoting a sense of multiple identities in middle school students could enhance their divergent thinking, a key component of creativity. In Experiment 1, 77 junior high school students were divided into multiple identities and physical trait condition groups. They were instructed to think about a child with multiple identities or physical traits. The results showed that there were no differences in divergent thinking (DT) scores between the two groups. In Experiment 2, we modified the priming method by asking participants to think about and write a description of the various identities or physical traits and employed a subjective top-scoring method to make up for shortcomings in the traditional scoring method when applied to originality. The results still showed no significant difference in scores between the identity and physical trait groups. Thus, the results of this study contradict those of previous research, which found that the identity group demonstrated significantly higher scores on a creativity test than did those in the physical trait group. Several potential factors affect this outcome, but it seems that priming to enhance divergent thinking is not particularly effective. Thus, the social priming effect should be pursued with caution regarding both replicability and generalizability.

3.
Front Neurosci ; 15: 698877, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34690669

RESUMO

Deception is a complex and cognitively draining dyadic process that simultaneously involves cognitive and emotional processes, both of which demand/capture attentional resources. However, few studies have investigated the allocation of attentional resources between cognitive and emotional processes during deception. The current study presented facial expressions of different valences to 36 participants. While an electroencephalogram was recording, they were asked to make either truthful or deceptive gender judgments according to preceding cues. The results showed that deceptive responses induced smaller P300 amplitudes than did truthful responses. Task-irrelevant negative emotional information (TiN) elicited larger P300 amplitudes than did task-irrelevant positive emotional information (TiP). Furthermore, the results showed that TiN elicited larger LPP amplitudes than did TiP in deceptive responses, but not in truthful ones. The results suggested that attentional resources were directed away to deception-related cognitive processes and TiN, but not TiP, was consistently able to compete for and obtain attentional resources during deception. The results indicated that TiN could disrupt with deception and may facilitate deception detection.

4.
Comput Intell Neurosci ; 2021: 8387382, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34475949

RESUMO

Image style transfer can realize the mutual transfer between different styles of images and is an essential application for big data systems. The use of neural network-based image data mining technology can effectively mine the useful information in the image and improve the utilization rate of information. However, when using the deep learning method to transform the image style, the content information is often lost. To address this problem, this paper introduces L1 loss on the basis of the VGG-19 network to reduce the difference between image style and content and adds perceptual loss to calculate the semantic information of the feature map to improve the model's perceptual ability. Experiments show that the proposal in this paper improves the ability of style transfer, while maintaining image content information. The stylization of the improved model can better meet people's requirements for stylization, and the evaluation indexes of structural similarity, cosine similarity, and mutual information value have increased by 0.323%, 0.094%, and 3.591%, respectively.


Assuntos
Sistemas de Dados , Redes Neurais de Computação , Humanos , Semântica
5.
Nanoscale ; 13(37): 15937-15951, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34523658

RESUMO

Vascular dysfunction and bacterial infection are key factors for the non-healing of diabetic ulcers. Growth factors and antibiotics seem to effectively target both issues. However, the short half-life and high cost of growth factors and the antibiotics resistance of bacteria greatly limit their further widespread applications. Novel strategies or agents with both angiogenic and antibacterial activities are urgently desirable. Copper peroxide (CuO2) nanodots were reported to be decomposed into Cu2+ and H2O2 under mild acid conditions (pH 5.5). Considering that both decomposed products are acknowledged antibacterial agents (Cu2+, H2O2) and angiogenesis activator (Cu2+), we believe that CuO2 nanodots are suitable for diabetic ulcer treatment because the pathological environment of infected chronic wounds is mildly acidic with pH 5.5-5.6. As expected, in vitro experiments showed that CuO2 nanodots possessed excellent bactericidal properties against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and even methicillin-resistant Staphylococcus aureus (MRSA). CuO2 nanodots induced the high expression of hypoxia-inducible factor (HIF-1α) and vascular endothelial growth factor (VEGF) in human umbilical vein endothelial cells (HUVECs), subsequently promoting the cell migration and tube formation for angiogenesis. In particular, CuO2 nanodots exhibited good dispersibility and sprayable behavior in water. In vivo experiments demonstrated that the spayed CuO2 nanodots in the wound area could effectively combat MRSA, reduce inflammation, promote angiogenesis, and consequently accelerate wound healing. Moreover, the sprayed CuO2 nanodots in the wound sites caused negligible system toxicity. This study provides proof-of-principle evidence for applying the sprayed CuO2 nanodots for infected diabetic ulcer treatment.


Assuntos
Diabetes Mellitus , Staphylococcus aureus Resistente à Meticilina , Antibacterianos/farmacologia , Bactérias , Cobre , Células Endoteliais da Veia Umbilical Humana , Humanos , Peróxido de Hidrogênio , Peróxidos , Úlcera , Fator A de Crescimento do Endotélio Vascular , Cicatrização
6.
Math Biosci Eng ; 18(5): 6652-6671, 2021 08 06.
Artigo em Inglês | MEDLINE | ID: mdl-34517550

RESUMO

The image super-resolution reconstruction method can improve the image quality in the Internet of Things (IoT). It improves the data transmission efficiency, and is of great significance to data transmission encryption. Aiming at the problem of low image quality in image super-resolution using neural networks, a self-attention-based image reconstruction method is proposed for secure data transmission in IoT environment. The network model is improved, and the residual network structure and sub-pixel convolution are used to extract the feature of the image. The self-attention module is used extract detailed information in the image. Using generative confrontation method and image feature perception method to improve the image reconstruction effect. The experimental results on the public data set show that the improved network model improves the quality of the reconstructed image and can effectively restore the details of the image.


Assuntos
Processamento de Imagem Assistida por Computador , Internet das Coisas , Redes Neurais de Computação
7.
Front Psychol ; 12: 684357, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34408705

RESUMO

Previous research has found that when gaze direction matches the underlying behavioral intent communicated by the expression of a specific emotion, it enhances or facilitates the perception of that emotion; this is called the shared signal hypothesis (SSH). Specifically, a direct gaze shares an approach-orientated signal with the emotions of anger and joy, whereas an averted gaze shares an avoidance-orientated signal with fear and sadness. In this research, we attempted to verify the SSH by using different materials on Asian participants. In Experiment 1 we employed photos of models exhibiting direct and averted gazes for rating tasks, in order to study the effects of gaze direction on participants' perception of emotion. In Experiment 2 we utilized smiling faces in a similar investigation. The results show that for neutral and smiling faces, a direct gaze (relative to a gaze of avoidance) increased the likelihood of a subject perceiving a happy mood; a gaze of avoidance increased the likelihood that anger and fear would be perceived. The effect of gaze direction on emotional expression perception was verified, but a "facilitating-impairing" pattern was not. The difference between our work and previous research may be attributable to the materials employed (which were more ecological), as well as the participants, who were from a different culture.

8.
Front Genet ; 12: 707299, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34349789

RESUMO

Esophageal cancer (EC) is the seventh most common tumor in the world, ranking the sixth leading cause of cancer death, with a 5-year survival rate of 15-25%. Therefore, reliable prognostic biomarkers are needed to effectively predict the prognosis of EC. In this study, the gene profile information of the EC cohort served as a training set, which was derived from TCGA and Immport databases. GO and KEGG enrichment analysis was performed on the differential genes in normal and tumor groups of EC. The immune genes in differentially expressed genes (DEGs) were further obtained for univariate and multivariate Cox and Lasso regression analysis, and 6 independent immune genes (S100A3, STC2, HSPA6, CCL25, GPER1, and OSM) associated with prognosis were obtained to establish an immune risk score signature (IRSS). The signature was validated using head and neck cancers (HNSC) and gastric cancer (GC)in upper gastrointestinal malignancies as validation sets. The Kaplan-Meier results showed that the prognosis of the high-risk group was significantly favorable than that of the low-risk group in both the training set (P < 0.001; HR = 3.68, 95% CI = 2.14-6.35) and the validation set (P = 0.010; HR = 1.43, 95% CI = 1.09-1.88). A nomogram combining multiple clinical information and IRSS was more effective than a single independent prognostic factor in predicting outcome. This study explored the potential link between immunity and EC, and established and validated prognostic biomarkers that can effectively predict the prognosis of EC, HNSC and GC based on six immune genes.

9.
Artigo em Inglês | MEDLINE | ID: mdl-34409847

RESUMO

AIMS: Hyperhomocysteinemia (HHcy) has been considered as a risk factor for cardiovascular disease (CVD), Alzheimer's disease (AD), nonalcoholic fatty liver (NAFL) and many other pathological conditions. Vitamin B6, Vitamin B12, and folate have been used to treat HHcy in clinic. However, at present clinical therapies of HHcy display unsatisfactory effects. Here, we would like to explore a new mechanism involved in Hcy metabolic disorders and a novel target for HHcy treatment. The key enzymes involved in Hcy metabolism deserve more insightful investigation. Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme regulating the intracellular Hcy metabolism. Until now, the effect of post-translational modification on bioactivity of MTHFR still remains unclear. This study aimed to explore the relationship between MTHFR S-sulfhydration and its bioactivity, and identify the contribution of elevated Hcy level on MTHFR bioactivity. RESULTS: By both in vivo and in vitro studies, we observed the following results: (1) The bioactivity of MTHFR was positively associated with its S-sulfhydration level; (2) MTHFR was modified at Cys32, Cys130, Cys131, Cys193, and Cys306 by S-sulfhydration under physiological conditions; (3) Hydrogen sulfide (H2S) deficiency caused the decrease of MTHFR S-sulfhydration level and bioactivity in HHcy, which resulted in further aggravation of HHcy; (4) H2S donors reversed the decreased bioactivity of MTHFR in HHcy, thus reduced the excessive Hcy level. Innovation and Conclusion: Our study suggested that H2S could improve MTHFR bioactivity by S-sulfhydration, which might provide a candidate therapeutic strategy for HHcy.

10.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 29(4): 1123-1128, 2021 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-34362491

RESUMO

OBJECTIVE: To observe the effects of down-regulation of long non-coding RNA HOX antisense intergenic RNA myeloid 1 (LncRNA-HOTAIRM1) to the proliferation and apoptosis of Jurkat in human leukemia T lymphocytes, and explore its mechanism. METHODS: Jurkat cells were cultured in vitro and randomly divided into control group, HOTAIRM1 siRNA-NC group and HOTAIRM1 siRNA group; the expressions of LncRNA-HOTAIRM1 mRNA, KIT receptor tyrosine kinase (KIT) mRNA and serine threonine kinase (AKT) mRNA in Jurkat cells were detected by real-time fluorescence quantification (RT-qPCR); the proliferation of Jurkat cells in each groups was detected by CCK-8 method; the apoptosis of Jurkat cells in each groups was detected by Annexin V-FITC/PI double staining; the expressions of KIT, AKT, p-KIT, p-AKT, B-lymphoma-2 gene (BCL-2) and Caspase-3 were detected by Western blot. RESULTS: Compared with the cells in the control group and HOTAIRM1 siRNA-NC group, the expression level of LncRNA-HOTAIRM1 mRNA, cell survival rate, expression levels of KIT mRNA, AKT mRNA, p-KIT, p-AKT and BCL-2 proteins in Jurkat cells in HOTAIRM1 siRNA group were significantly lower (P<0.05), while the expression level of Cleared Caspase-3 protein and Jurkat cell apoptosis rate were significantly higher (P<0.05). CONCLUSION: LncRNA-HOTAIRM1 may inhibit Jurkat cell proliferation and induce apoptosis through KIT/AKT signaling pathway.


Assuntos
RNA Longo não Codificante , Apoptose , Proliferação de Células , Regulação para Baixo , Humanos , Células Jurkat , Proteínas Proto-Oncogênicas c-akt/metabolismo , RNA Longo não Codificante/genética
11.
Cancer Cell Int ; 21(1): 362, 2021 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-34238288

RESUMO

BACKGROUND: To rummage autophagy-related prognostic, diagnostic, and therapeutic biomarkers in cervical cancer (CC). METHODS: The RNA-sequence and clinical information were from the TCGA and GTEx databases. We operated Cox regression to determine signatures related to overall survival (OS) and recurrence-free survival (RFS) respectively. The diagnostic and therapeutic effectiveness of prognostic biomarkers were further explored. RESULTS: We identified nine (VAMP7, MTMR14, ATG4D, KLHL24, TP73, NAMPT, CD46, HGS, ATG4C) and three risk signatures (SERPINA1, HSPB8, SUPT20H) with prognostic values for OS and RFS respectively. Six risk signatures (ATG4C, ATG4D, CD46, TP73, SERPINA1, HSPB8) were selected for qPCR. We screened five prognostic signatures(ATG4C, CD46, HSPB8, MTMR14, NAMPT) with diagnostic function through the GEO database. Correlation between our models and treatment targets certificated the prognostic score provided a reference for precision medicine. CONCLUSIONS: We constructed OS and RFS prognostic models in CC. Autophagy-related risk signatures might serve as diagnostic and therapeutic biomarkers.

12.
AoB Plants ; 13(4): plab033, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34234936

RESUMO

Soil heterogeneity significantly affects plant dynamics such as plant growth and biomass. Most studies developed soil heterogeneity in two dimensions, i.e. either horizontally or vertically. However, soil heterogeneity in natural ecosystems varies both horizontally and vertically, i.e. in three dimensions. Previous studies on plant biomass and biomass allocation rarely considered the joint effects of soil heterogeneity and species composition. Thus, to investigate such joint effects on plant biomass and biomass allocation, a controlled experiment was conducted, where three levels of soil heterogeneity and seven types of species compositions were applied. Such soil heterogeneity was developed by filling nutrient-rich and nutrient-poor substrates in an alternative pattern in pots with different patch sizes (small, medium or large), and species compositions was achieved by applying three plant species (i.e. Festuca elata, Bromus inermis, Elymus breviaristatus) in all possible combinations (growing either in monoculture or in mixtures). Results showed that patch size significantly impacted plant biomass and biomass allocation, which differed among plant species. Specially, at the pot scale, with increasing patch size, shoot biomass decreased, while root biomass and R:S ratio increased, and total biomass tended to show a unimodal pattern, where the medium patch supported higher total biomass. Moreover, at the substrate scale, more shoot biomass and total biomass were found in nutrient-rich substrate. Furthermore, at the community scale, two of the three target plant species growing in monoculture had more shoot biomass than those growing together with other species. Thus, our results indicate soil heterogeneity significantly affected plant biomass and biomass allocation, which differ among plant species, though more research is needed on the generalization on biomass allocation. We propose that soil heterogeneity should be considered more explicitly in studies with more species in long-term experiments.

13.
Nanomaterials (Basel) ; 11(6)2021 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-34205616

RESUMO

d-cysteine (d-cys) has been demonstrated to possess an extraordinary antibacterial activity because of its unique steric configuration. However, inefficient antibacterial properties seriously hinder its wide applications. Here, cysteine-functionalized gold nanoparticles (d-/l-Au NPs) were prepared by loading d-/l-cysteine on the surface of gold nanoparticles for the effective inhibition of Escherichia coli (E. coli) in vitro and in vivo, and the effects on the intestinal microflora in mice were explored during the treatment of E. coli infection in the gut. We found that the antibacterial activity of d-/l-Au NPs was more than 2-3 times higher than pure d-cysteine, l-cysteine and Au NPs. Compared with l-Au NPs, d-Au NPs showed the stronger antibacterial activity, which was related to its unique steric configuration. Chiral Au NPs showed stronger destructive effects on cell membrane compared to other groups, which further leads to the leakage of the cytoplasm and bacterial cell death. The in vivo antibacterial experiment illustrated that d-Au NPs displayed impressive antibacterial activity in the treatment of E. coli-infected mice comparable to kanamycin, whereas they could not affect the balance of intestinal microflora. This work is of great significance in the development of an effective chiral antibacterial agent.

14.
Exp Brain Res ; 239(8): 2561-2567, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34185099

RESUMO

Remote ischemic conditioning (RIC), as an emerging protective method, might be used clinically to prevent ischemia-reperfusion injury (IRI) in ischemic stroke. In this study, we aim to investigate whether RIC performed either during brain ischemia or after reperfusion has a protective effect and further explore the mechanistic basis for the protective effects of RIC against IRI in an aged rat model. We investigated brain IRI in 16-18 months old SD rats. Animals underwent: (i) sham laparotomy, (ii) brain IRI, (iii) brain IRI + RIC during ischemia (IRI + RIperC), or (iv) brain IRI + RIC after reperfusion (IRI + RIpostC). RIC consists of three cycles of 10 min of hind limb ischemia followed by 10 min reperfusion. After 24 h of reperfusion, the infarct size, neurological deficit scores and brain oedema were assessed in all groups. The levels of IL-1ß, IL-6, TNF-α were measured by ELISA. The mRNA and protein expressions of TLR4, MyD88, TRAF6 and NF-κB were detected by RT-PCR and western blot. Both RIperC and RIpostC treatment attenuated the IRI-induced neuronal injury, reflected by reductions in the infarct size, neurological deficit scores and brain oedema. RIperC and RIpostC also can decrease the concentration of IL-1ß, IL-6, TNF-α in IRI. From the results of RT-PCR and western blot, we found that RIC decreased the mRNA and protein expression of TLR4, MyD88, TRAF6 and NF-κB compared to that in the IRI group. The present study suggested that RIC protected aged rats against IRI, and this protective effect might be mediated by inhibiting the TLR-4/MyD88/TRAF-6/NF-κB signaling pathway.


Assuntos
Lesões Encefálicas , Traumatismo por Reperfusão , Animais , Isquemia , Fator 88 de Diferenciação Mieloide/metabolismo , Ratos , Ratos Sprague-Dawley , Transdução de Sinais , Receptor 4 Toll-Like/genética , Receptor 4 Toll-Like/metabolismo
15.
Biochem Biophys Res Commun ; 562: 69-75, 2021 07 12.
Artigo em Inglês | MEDLINE | ID: mdl-34038755

RESUMO

XBP1 is a basic leucine zipper (bZIP) transcription factor and a key mediator of the endoplasmic reticulum (ER) stress-activated unfolded protein response (UPR). XBP1-mediated transcription facilitates cell adaptation to ER stress and also promotes tumor progression, while suppressing anti-tumor immunity. Here we report a novel XBP1 variant, namely XBP1 variant 1 (XBP1v1, Xv1 for short), that is specifically required for survival of cancer cells. Xv1 contains a cryptic first exon that is conserved only in humans and great apes. Comparing to XBP1, Xv1 encodes a protein with a different N-terminal sequence containing 25 amino acids. Analysis of RNAseq database reveals that Xv1 is broadly expressed across cancer types but almost none in normal tissues. Elevated Xv1 expression is associated with poor survival of patients with several types of cancer. Knockdown of Xv1 induces death of multiple cancer cell lines but has little effect on non-cancerous cells in vitro. Moreover, knockdown of Xv1 also inhibits growth of a xenograft breast tumor in mice. Together, our results indicate that Xv1 is essential for survival of cancer cells.


Assuntos
Variação Genética , Neoplasias/genética , Neoplasias/patologia , Proteína 1 de Ligação a X-Box/genética , Animais , Linhagem Celular Tumoral , Sobrevivência Celular/genética , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Camundongos Nus , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ensaios Antitumorais Modelo de Xenoenxerto
16.
Epigenomics ; 13(11): 891-907, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33955785

RESUMO

Aim: To explore tumor immune microenvironment and identify immune prognostic-related circRNAs in cervical cancer. Materials & methods: RNA-seq in combination with bioinformatics were performed to establish a prognostic risk model and a circRNAs-miRNAs-CXCL8 network. Results: High-risk group correlated with poor survival outcome, and had lower PD-1 immunogenicity. Additionally, CXCL8 could distinguish normal tissue, low- and high-risk tumor tissues, the expression of which showed an increasing trend among the three groups. RNA-seq and bioinformatics indicated that circRNAs like hsa_circ_0025721 might upregulate CXCL8 through sponging miRNAs including hsa-miR-4428. Conclusion: We constructed an immune risk model related with CD8 T cells to predict the cervical cancer patients' prognosis and explored the abnormal expression mechanism of CXCL8 through the ceRNA mechanism.

17.
Compr Rev Food Sci Food Saf ; 20(3): 2626-2659, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33876887

RESUMO

Decontamination of meat is commonly practiced to get rid of or decrease the microbial presence on the meat surface. Dielectric barrier discharge cold atmospheric plasma (DBD-CAP) as innovative technology is a food microbial inactivation technique considered in high regard by food scientists and engineers in present times. However, cold atmospheric plasma application is at the experimental stage, due to lack of sufficient information on its mode of action in inactivating microbes, food shelf-life extensibility, whereas, the nutritional value of food is preserved. In this review, we have appraised recent work on DBD-CAP concerning the decontamination treatment of meat products, highlighting the processing value results on the efficacy of the DBD-CAP microbial inactivation technique. Also, the paper will review the configurations, proposed mechanisms, and chemistry of DBD-CAP. Satisfactory microbial inactivation was observed. In terms of DBD-CAP application on sensory evaluation, inferences from reviewed literature showed that DBD has no significant effect on meat color and tenderness, whereas in contrast, TBARS values of fresh and processed meat are affected. DBD seems economically efficient and environmentally sustainable.


Assuntos
Produtos da Carne , Gases em Plasma , Armazenamento de Alimentos , Carne , Viabilidade Microbiana
18.
Acta Neurol Belg ; 2021 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-33683634

RESUMO

To evaluate the spontaneous neuronal activities and the changes of brain functional network in patients with vestibular migraine (VM). Three groups including18 patients with VM, 21 patients with migraine without aura (MWoA) and 21 healthy controls (HCs) underwent the scanning of the resting-state fMRI. Covariance analysis and bonferroni multiple comparisons were used to obtain brain regions with significant differences in amplitude of low-frequency fluctuation (ALFF) values. Furthermore, the brain regions with the most significant differences of ALFF values were recognized as a region of interest (ROI) and functional connectivity (FC) analysis was performed in these regions. (1) ALFF: Compared with HCs, patients with VM showed significantly lower ALFF in the right putamen (P < 0.05), and significantly higher ALFF in the right lingual gyrus (P < 0.05). In addition, compared with MWoA patients, patients with VM showed significantly higher ALFF in the right lingual gyrus (P < 0.05). (2) Compared with HCs, VM patients showed significantly higher FC among the cerebellum, the left dorsolateral superior frontal gyrus and the right putamen (P < 0.05) but significantly lower FC among the left median cingulate, paracingulate gyri and the right putamen (P < 0.05). Compared with MWoA patients, VM patients showed significantly higher FC between the cerebellum and the right putamen (P < 0.05) but significantly lower FC among the left median cingulate, paracingulate gyri and the right putamen (P < 0.05). There are functional abnormalities in nociceptive, vestibular and visual cortex regions in patients with VM during the interictal period.

19.
Food Chem ; 351: 129278, 2021 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-33631612

RESUMO

The poor gel strength and microbial infection of conventional chicken myofibrillar protein (CMP) gels have severely limited the application. Here, plasma activated water (PAW) instead of normal water was used to prepare CMP gels. PAW prepared by treating deionized water with plasma jet was incubated with CMPs and followed by heating to prepare CMP gels. Effects of PAW on CMP gels were assessed in terms of basic physicochemical properties, network structure, and antibacterial activity. The results showed that PAW treatment accelerated the aggregation of CMPs and increased the strength and water holding capacity of CMP gels. Due to the presence of NO and NO2 free radicals in PAW, the prepared CMP gels were endowed with antibacterial activity against Salmonella Enteritidis and Staphylococcus aureus. The new method of PAW-induced CMP gels will have the prospect of improving the quality of gels and extending the shelf life of chicken gel products.


Assuntos
Antibacterianos/química , Antibacterianos/farmacologia , Galinhas , Proteínas Musculares/química , Proteínas Musculares/farmacologia , Gases em Plasma/química , Água/química , Animais , Géis
20.
Meat Sci ; 176: 108456, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33621829

RESUMO

The objective of this work was to investigate the changes in color, metmyoglobin reducing activity (MRA), and lipid oxidation (TBARS) and their relationships in beef patties treated by dielectric barrier discharge cold plasma (DBD-CP) at different voltages and frequencies during storage at 4 °C. DBD-CP treatment, including treatment voltages and frequencies, caused the decrease of a⁎ value and MRA (P < 0.05), promoted the accumulation of metmyoglobin (P < 0.05), and significantly increased TBARS values (P < 0.05). It was the first time revealed that the effect of treatment voltage on the quality of beef patty was greater than that of frequency. In addition, there were significant and strong positive correlations between a⁎ value and MRA and negative correlations between a⁎ value, metmyoglobin (MMb)% and TBARS. Taken together, Data demonstrated that DBD-CP treatment can significantly affect meat redness, Mb contents, MRA, and lipid oxidation in ground beef during storage, and accelerate discoloration and lipid oxidation of meat.


Assuntos
Cor , Conservação de Alimentos/métodos , Produtos da Carne/análise , Metamioglobina/metabolismo , Gases em Plasma , Animais , Bovinos , Manipulação de Alimentos/métodos , Armazenamento de Alimentos , Substâncias Reativas com Ácido Tiobarbitúrico/análise
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