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1.
J Comp Pathol ; 187: 27-39, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34503652

RESUMO

Akabane, Aino and Peaton viruses are closely related arthropod-borne viruses in the genus Orthobunyavirus of the family Peribunyaviridae that can cause congenital abnormalities in cattle, sheep and goats. East Asian Akabane virus strains are subdivided into genogroups Ⅰ and Ⅱ, and the former can also cause non-suppurative encephalomyelitis in post-natal animals. Specific detection of the infecting virus in tissues is essential for accurate diagnosis. Immunohistochemistry (IHC) has been used to identify viral antigen but cannot always detect specific viruses due to potential cross-reactivity of the primary antisera. We compared in-situ hybridization (ISH), based on the use of cocktail probe sets targeted at the RNA of each virus, with IHC for the detection of the specific viruses in tissues of suckling rats inoculated intracerebrally with Akabane (KM-1 or OBE-1 strains), Aino or Peaton viruses at 3 or 7 days of age. Most inoculated rats developed severe neurological signs and histopathological brain lesions including necrosis, spongy degeneration and non-suppurative inflammation. A rabbit polyclonal antiserum immunolabelled antigen of all three viruses within the lesions, whereas ISH specifically detected RNA of each individual virus. The distribution of viral RNA was comparable to that of viral antigens, but tended to be more widespread, especially in immature nervous tissue. Viral antigen and RNA were detected in skeletal muscle and heart of the rats infected with the KM-1 strain of Akabane virus but not with any of the other viruses. This study demonstrates the value of ISH detection of these viruses in a rat model and may prove useful for clarification of the pathogenesis of post-natal arbovirus infection.

2.
Artigo em Inglês | MEDLINE | ID: mdl-34463150

RESUMO

The circulation of arboviruses in livestock ruminants has often gone unrecognized owing to the fact that a significant percentage of arboviruses probably induce subclinical infections and/or negligible symptoms in infected animals. To determine the current situation of arbovirus circulation in the Yaeyama Islands, attempts to isolate viruses from bovine blood samples collected between 2014 and 2019 have been made. In total, 308 blood samples were collected during the study period, and 43 of them induced cytopathic effects (CPEs) in cell cultures. The identification of the CPE agents was performed by reported RT-PCR assays and a high-throughput analysis with a next-generation sequencing platform. The obtained viruses consisted of an orthobunyavirus (Peaton virus), Culicoides-borne orbiviruses (bluetongue virus serotypes 12 and 16, epizootic hemorrhagic disease virus [EHDV] serotypes 5, 6, and 7, D'Aguilar virus, and Bunyip Creek virus), and potential mosquito-borne orbiviruses (Yunnan orbivirus, Guangxi orbivirus, and Yonaguni orbivirus). Most of the orbiviruses were recovered from washed blood cells with mosquito cell cultures, suggesting that this combination was more efficient than other combinations such as plasma/blood cells and hamster cell lines. This marked the first time that the isolation of EHDV serotypes 5 and 6 and three potential mosquito-borne orbiviruses was recorded in Japan, showing a greater variety of orbiviruses on the islands than previously known. Genetic analysis of the isolated orbiviruses suggested that the Yaeyama Islands and its neighboring regions were epidemiologically related. Some of the viruses, especially the potential mosquito-borne orbiviruses, were isolated during several consecutive years, indicating their establishment on the islands.

3.
J Vet Med Sci ; 83(9): 1378-1388, 2021 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-34248104

RESUMO

Epizootic hemorrhagic disease (EHD) is an arthropod-borne disease of wild and domestic ruminants caused by the EHD virus (EHDV). To date, seven EHDV serotypes have been identified. In Japan, strain Ibaraki of EHDV serotype 2 has caused outbreaks of Ibaraki disease in cattle. In addition, EHDV serotype 7 (EHDV-7) has caused large-scale EHD epizootics. In mid-September 2016, eight cattle at a breeding farm in Fukuoka Prefecture, Japan developed fever. Since EHDV-7 was detected in sentinel cattle in western Japan in 2016, we suspected that the cause of this fever might be an EHDV-7 infection. In this study, we tested cattle for EHDV-7 and some other viruses. Consequently, EHDV was isolated from washed blood cells collected from three of the eight cattle, and genetic analysis of genome segment 2 revealed that this isolate was EHDV-7. Moreover, all affected cattle tested positive for anti-EHDV-7 neutralizing antibodies. Our results suggest that the fever was caused by EHDV-7 infection. In addition, we modified a conventional reverse transcription polymerase chain reaction assay for the specific detection of EHDV. This modified assay could detect various strains of EHDV isolated in Japan, Australia, and North America. Furthermore, the assay permitted the detection of EHDV-7 in blood cells collected from seven of the eight cattle. We believe that this modified assay will be a useful tool for the diagnosis of EHD.


Assuntos
Doenças dos Bovinos , Vírus da Doença Hemorrágica Epizoótica , Infecções por Reoviridae , Animais , Bovinos , Doenças dos Bovinos/diagnóstico , Doenças dos Bovinos/epidemiologia , Vírus da Doença Hemorrágica Epizoótica/genética , Japão/epidemiologia , Infecções por Reoviridae/diagnóstico , Infecções por Reoviridae/epidemiologia , Infecções por Reoviridae/veterinária , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Transcrição Reversa , Sorogrupo
4.
Arch Virol ; 166(4): 1151-1156, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33547486

RESUMO

Tibet orbivirus (TIBOV) was initially isolated in Tibet in 2009 and subsequently in Guangdong, Hunan, and Yunnan, China. We document the first isolation of TIBOV outside of China: two TIBOV isolates from Culicoides collected in 2009 and 2010 in Kagoshima, Japan. Their complete genome sequences were also determined. Our results suggest that the two virus isolates are of novel serotypes, evident by variability within genome segment 2 encoding VP2. These new putative TIBOV serotypes will help with future virus surveillance and with the evaluation of its potential to cause disease in domestic ruminants.


Assuntos
Genoma Viral/genética , Orbivirus/genética , Orbivirus/isolamento & purificação , Animais , Ceratopogonidae/virologia , Genômica , Japão , Orbivirus/classificação , Filogenia , RNA Viral/genética , Homologia de Sequência , Sorogrupo , Proteínas Virais/genética
5.
Arch Virol ; 165(12): 2903-2908, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32894348

RESUMO

A novel orbivirus (genus Orbivirus, family Reoviridae), designated Yonaguni orbivirus (YONOV), was isolated from bovine blood collected on a subtropical island of Japan in 2015. The YONOV genome (20,054 nucleotides in total) has a coding arrangement similar to those of mosquito-borne orbiviruses. YONOV has a close genetic relationship to mosquito-borne orbiviruses, especially to Mobuck virus (MBV), which was isolated in North America. However, YONOV and MBV share less than 74% nucleotide sequence identity in the major subcore protein (T2) coding sequence, which satisfies the criterion for species demarcation. It is still uncertain whether YONOV should be assigned to a novel species in the genus Orbivirus.


Assuntos
Genoma Viral , Orbivirus/classificação , Orbivirus/isolamento & purificação , Filogenia , Infecções por Reoviridae/veterinária , Proteínas Virais/genética , Animais , Bovinos/virologia , Culicidae/virologia , Japão , Fases de Leitura Aberta , Infecções por Reoviridae/virologia , Análise de Sequência de DNA
6.
Microbiol Resour Announc ; 9(39)2020 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-32972940

RESUMO

Akabane virus (AKAV) (genus Orthobunyavirus, family Peribunyaviridae) is an arthropod-borne virus that causes congenital abnormalities in ruminants. Here, we report the complete genome sequences of two AKAV strains causing nonsuppurative encephalomyelitis in cattle by postnatal infection in Japan.

7.
Front Vet Sci ; 7: 168, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32318588

RESUMO

Epizootic congenital abnormalities caused by Akabane, Aino, and Chuzan viruses have damaged the reproduction of domestic ruminants in East Asia for many years. In the past, large outbreaks of febrile illness related to bovine ephemeral fever and Ibaraki viruses severely affected the cattle industry in that region. In recent years, vaccines against these viruses have reduced the occurrence of diseases, although the viruses are still circulating and have occasionally caused sporadic and small-scaled epidemics. Over a long-term monitoring period, many arboviruses other than the above-mentioned viruses have been isolated from cattle and Culicoides biting midges in Japan. Several novel arboviruses that may infect ruminants (e.g., mosquito- and tick-borne arboviruses) were recently reported in mainland China based on extensive surveillance. It is noteworthy that some are suspected of being associated with cattle diseases. Malformed calves exposed to an intrauterine infection with orthobunyaviruses (e.g., Peaton and Shamonda viruses) have been observed. Epizootic hemorrhagic disease virus serotype 6 caused a sudden outbreak of hemorrhagic disease in cattle in Japan. Unfortunately, the pathogenicity of many other viruses in ruminants has been uncertain, although these viruses potentially affect livestock production. As global transportation grows, the risk of an accidental incursion of arboviruses is likely to increase in previously non-endemic areas. Global warming will also certainly affect the distribution and active period of vectors, and thus the range of virus spreads will expand to higher-latitude regions. To prevent anticipated damages to the livestock industry, the monitoring system for arboviral circulation and incursion should be strengthened; moreover, the sharing of information and preventive strategies will be essential in East Asia.

8.
J Vet Med Sci ; 81(10): 1496-1503, 2019 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-31447461

RESUMO

A surveillance of Culicoides biting midges with light suction traps was conducted in the northern region of Honshu, main island of Japan, during the summers and autumns of 2009 and 2010. A total of 106 trap collections across 37 cattle farms were investigated for the structure and distribution of Culicoides species. Forty-thousand and one hundred forty-nine specimens of Culicoides biting midges were identified at the species level, and ≥19 species were included in the specimens. Culicoides oxystoma, which is a known major vector of Akabane virus (AKAV), appeared not to have expanded in northern Honshu during the surveillance. Of the potential AKAV vectors suggested by a previous laboratory experiment, C. tainanus and C. punctatus widely infested cowsheds across northern Honshu. The AKAV circulation was confirmed by serological surveillance of sentinel cattle in northern Honshu during the summer and autumn of 2010 and, consequently, >200 calves affected by the virus were identified as of spring 2011. Our surveillance demonstrated that C. tainanus and C. punctatus were widely spread and often dominated at cattle farms in/around the seroconverted regions, and our results thus suggest that these species played a critical role in the AKAV transmission in 2010. Because the distribution ranges of C. tainanus and C. punctatus cover almost all of mainland Japan, a potential risk of AKAV transmission might be expected even in areas outside the range of C. oxystoma.


Assuntos
Doenças dos Bovinos/transmissão , Doenças dos Bovinos/virologia , Ceratopogonidae/virologia , Insetos Vetores/virologia , Orthobunyavirus , Animais , Bovinos , Surtos de Doenças , Japão/epidemiologia , Testes Sorológicos
9.
Vet Ital ; 55(1): 81-89, 2019 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-30951185

RESUMO

Viruses of the Simbu serogroup are arboviruses that are known to cause outbreaks of abortion, stillbirth and congenitally deformed neonates. This study presents the results of antibody screening of Simbu serogroup viruses in heifers born in Israel after October 2013, and in adult milking cows born before May 2012. Thirteen dairy cattle farms in five regions, and one sheep flock, entered this study. Serum samples that were found to be positive by ELISA were further tested by specific virus- neutralization test against a panel of Simbu serogroup viruses including Akabane, Aino, Sathuperi, Shamonda, and Peaton viruses. Antibody detection in lactating adult cows revealed that several viruses were circulating in Israel between 2008-2014. Moreover, during autumn 2014 the heifers became serum-positive after being exposed to more than one Simbu serogroup virus concurrently. The results of this study shed new light on Simbu virus infections in Israel, and may contribute to the epidemiology of the Simbu serogroup around the Mediterranean Basin in general.


Assuntos
Infecções por Bunyaviridae/veterinária , Doenças dos Bovinos/virologia , Vírus Simbu/fisiologia , Animais , Infecções por Bunyaviridae/epidemiologia , Infecções por Bunyaviridae/virologia , Bovinos , Doenças dos Bovinos/epidemiologia , Feminino , Israel/epidemiologia , Sorogrupo , Ovinos , Doenças dos Ovinos/epidemiologia , Doenças dos Ovinos/virologia , Vírus Simbu/classificação , Vírus Simbu/genética
10.
J Med Entomol ; 56(2): 533-539, 2019 02 25.
Artigo em Inglês | MEDLINE | ID: mdl-30418597

RESUMO

To test their virus susceptibility and capacity as transmission vectors, Japanese Culicoides species were artificially fed a mixture of Akabane virus (AKAV) and bovine blood, and virus recovery was attempted from infected midges 9-11 d post-exposure. Culicoides tainanus, C. punctatus, C. humeralis, C. jacobsoni, C. oxystoma, and C. asiana were found to be orally susceptible to AKAV. Virus titers in single infected midges of C. tainanus, C. oxystoma, C. punctatus, and C. jacobsoni ranged from 100.75 to 104.0 TCID50 (tissue culture infectious dose). The titers in the infected C. oxystoma were significantly higher than those in the other infected species. Viral RNA was detected from both midges testing positive and those testing negative for infectious virus particles, but the viral RNA copies in the infectious virus-negative midges were significantly lower than those in the infectious virus-positive midges. Lower viral amplification, limited dissemination or both caused by tissue barriers might occur in infected midges from which infectious viruses were undetectable. A fully disseminated infection was developed in orally infected C. oxystoma and C. tainanus. This finding indicates their capacity to transmit AKAV, assuming that salivary gland barriers have limited effects on viral entry to and replication in salivary gland tissue. This result also suggests that the other orally susceptible species are potentially competent for AKAV transmission and would be considered active vectors of its spread.


Assuntos
Ceratopogonidae/virologia , Insetos Vetores/virologia , Orthobunyavirus/fisiologia , Animais , Comportamento Alimentar
11.
Vet Med Sci ; 5(1): 87-92, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30394673

RESUMO

Simbu serogroup are arbo- viruses which are mainly transmitted by Culicoides. Two members of the Simbu serogroup, Akabane and Shuni viruses, have been isolated from congenitally malformed ruminants in Israel. A recent serosurvey revealed that Israeli ruminants have been exposed to several additional Simbu viruses, including Shamonda and Sathuperi that seems to be circulating in Israel. In April 2017, an apparently healthy one-month-old male calf was transferred to the Kimron Veterinary Institute. A few days later, the calf was reported to be slow to respond to its surroundings and was not able to feed on its own. Blindness was observed upon clinical examination. RNA of the small, medium and large segments of Simbu serogroup viruses were amplified and sequenced from the testis tissues and from the Cerebrospinal fluid (CSF). During post-mortem examination, hydranencephaly was defined. Phylogenetic analysis of all three segments of Simbu serogroup viruses showed that the sequences detected in the Israeli calf were virtually identical to Peaton virus (PEAV). PEAV was also detected in two pools of Culicoides imicola trapped at two different locations in Israel. This is the first genomic detection of PEAV outside Australia and Japan. These results are of epidemiological significance, as they demonstrate that PEAV is circulating in Israel and affects cattle. Consequently, these results are also of relevance to a potential spread of Simbu serogroup viruses into Europe.


Assuntos
Infecções por Bunyaviridae/veterinária , Hidranencefalia/veterinária , Vírus Simbu/isolamento & purificação , Animais , Encéfalo/patologia , Encéfalo/virologia , Infecções por Bunyaviridae/epidemiologia , Infecções por Bunyaviridae/virologia , Bovinos , Genoma Viral , Hidranencefalia/patologia , Hidranencefalia/virologia , Masculino , Vírus Simbu/genética
12.
J Vet Diagn Invest ; 30(6): 855-861, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30204057

RESUMO

Peaton virus (PEAV; family Peribunyaviridae, genus Orthobunyavirus) appears to be capable of producing congenital malformations in ruminants; however, its pathogenicity remains unknown given its relatively low incidence. We evaluated the relationship between congenital abnormalities of calves and PEAV infection by serologic, epidemiologic, pathologic, and virologic investigations using specimens from 31 malformed calves in the years 1996-2016 in Japan. Antibody testing was carried out for known teratogenic viruses, including Akabane, Aino, Chuzan, and bovine viral diarrhea viruses, in the precolostral sera of these abnormal calves, but all results were negative. However, all 31 malformed calves were positive for antibodies against PEAV. A PEAV-specific gene was amplified from central nervous system tissues from a stillborn calf delivered in April 2007, and its nucleotide sequence was identical with that of PEAV isolated from healthy sentinel cattle in September 2006. These findings indicate that PEAV can cause bovine congenital anomalies.


Assuntos
Infecções por Bunyaviridae/veterinária , Doenças dos Bovinos/patologia , Bovinos/anormalidades , Anormalidades Congênitas/veterinária , Orthobunyavirus/fisiologia , Animais , Anticorpos Antivirais/sangue , Infecções por Bunyaviridae/patologia , Infecções por Bunyaviridae/virologia , Doenças dos Bovinos/virologia , Anormalidades Congênitas/patologia , Anormalidades Congênitas/virologia , Japão
13.
Virus Genes ; 54(5): 729-732, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30069670

RESUMO

Two virus strains, tentatively designated as ON-6/P/05 and ON-7/E/05, were isolated from blood samples of healthy cattle in the Yaeyama Islands, located in the southwestern-most region of Japan, in 2005. Ultrastructural observations of infected baby hamster (BHK-21) cells revealed that the viruses had features consistent with those of orbivirus. As with other orbiviruses, the viral genome consists of 10 double-stranded RNA segments. The full genome sequence of ON-6/P/05 was determined and shared high nucleotide and amino acid identities (90.07-98.22% nucleotide identity; 96.16-99.72% amino acid identity) with that of Sathuvachari virus (SVIV), a member of the species Sathuvachari virus of the genus Orbivirus, originally isolated from starlings collected in southern India in 1963. The sequence of segment two of ON-7/E/05 was identical to that of ON-6/P/05. The isolation of SVIV from cattle also indicated that the virus has a wider host range than previously thought. The potential pathogenicity of SVIV in domestic animals should be considered in future disease surveillance within its distribution range.


Assuntos
Bovinos/virologia , Genoma Viral , Orbivirus/genética , Orbivirus/isolamento & purificação , Animais , Linhagem Celular , Cricetinae , Feminino , Japão , Tipagem Molecular , Orbivirus/classificação , Especificidade da Espécie
14.
Emerg Infect Dis ; 24(5): 902-905, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-29664367

RESUMO

During October-December 2015, an epizootic hemorrhagic disease outbreak occurred in cattle in Japan. Forty-six animals displayed fever, anorexia, cessation of rumination, salivation, and dysphagia. Virologic, serologic, and pathologic investigations revealed the causative agent was epizootic hemorrhagic disease virus serotype 6. Further virus characterization is needed to determine virus pathogenicity.


Assuntos
Doenças dos Bovinos/virologia , Vírus da Doença Hemorrágica Epizoótica/isolamento & purificação , Infecções por Reoviridae/veterinária , Animais , Bovinos , Doenças dos Bovinos/epidemiologia , Surtos de Doenças , Vírus da Doença Hemorrágica Epizoótica/genética , Japão/epidemiologia , Filogenia , Infecções por Reoviridae/epidemiologia , Infecções por Reoviridae/virologia
15.
Trop Anim Health Prod ; 50(1): 209-215, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28932950

RESUMO

Akabane virus, the pathogen-causing Akabane disease, is an arthropod-borne virus (arbovirus) transmitted by the Culicoides biting midge. A nationwide serological surveillance program for bovine arboviral diseases, including Akabane disease, has been established in Japan to monitor the circulation of arboviruses by targeting sentinel calves. Okinawa, which is located in the southwestern-most region of Japan, is a high-risk area for incursion of arboviruses. The aim of the present study was to identify the meteorological factors related to farm-level seroconversion of Akabane virus by analyzing the serological surveillance data for sentinel calves collected in Okinawa between 2007 and 2015. Rainfall in winter, spring, and autumn was positively associated with seroconversion. Adequate rainfall seems to keep the soil in a suitably moist state for growth and survival of biting midges. Maximum temperature in winter was also positively associated with seroconversion in sentinel calves. The warmer temperatures in winter may provide conditions suitable for shortening the larval development cycle and increase production of adult midges. Our findings indicate that meteorological factors such as temperature and rainfall may be important factors that produce circumstances conducive to effective transmission of the virus between vectors and the host. The results of this study provide a better understanding of the circulation of arboviruses and offer suggestions for developing better surveillance and measures to prevent arboviral disease.


Assuntos
Infecções por Arbovirus/veterinária , Doenças dos Bovinos/epidemiologia , Tempo (Meteorologia) , Animais , Infecções por Arbovirus/epidemiologia , Infecções por Arbovirus/transmissão , Arbovírus , Bovinos , Doenças dos Bovinos/transmissão , Ceratopogonidae/virologia , Insetos Vetores/virologia , Japão/epidemiologia , Fatores de Risco , Estações do Ano , Soroconversão
16.
Infect Genet Evol ; 53: 38-46, 2017 09.
Artigo em Inglês | MEDLINE | ID: mdl-28506840

RESUMO

We characterized genome segments 2, 3 and 6 (Seg-2, Seg-3 and Seg-6) of 11 Japanese strains of epizootic hemorrhagic disease (EHD) virus (EHDV) isolated in 1985-2013. The Japanese strains were divisible into two groups based on phylogenetic analyses of the nucleotide sequences of Seg-2 and Seg-6. In both of the phylogenetic trees based on Seg-2 and Seg-6, seven of the 11 Japanese strains were grouped together with EHDV-2 and EHDV-7 strains, and the other four Japanese strains were grouped with EHDV-1 strains. The phylogenetic analysis of Seg-2 among EHDV strains identified 10 of the 11 Japanese strains as EHDV-1, EHDV-2 or EHDV-7. The other Japanese strain, ON-4/B/98, isolated from an asymptomatic cow in 1998 was in the same group as the EHDV-2 and EHDV-7 strains in the phylogenetic trees based on Seg-2 and Seg-6, but the results suggested that the strain belongs to another serotype. We thus conducted a serum neutralization test to identify that serotype by using anti-EHDV-2 and anti-EHDV-7 rabbit sera. We observed that the ON-4/B/98 strain was not sufficiently neutralized by any of the antisera, which suggests that the strain could be assigned into a new serotype, tentatively named 'EHDV-10.' Sequences of Seg-3 were also determined, and all of the Japanese strains were grouped together with Australian strains, suggesting that the Japanese strains are a part of EHDV distributed in the Asia-Pacific region. The data obtained herein would be beneficial for the diagnosis and prevention of EHD in Japan and neighboring countries.


Assuntos
Doenças dos Bovinos/epidemiologia , Genoma Viral , Vírus da Doença Hemorrágica Epizoótica/genética , Filogenia , RNA Viral/genética , Infecções por Reoviridae/veterinária , Animais , Bovinos , Doenças dos Bovinos/virologia , Genótipo , Vírus da Doença Hemorrágica Epizoótica/classificação , Vírus da Doença Hemorrágica Epizoótica/imunologia , Vírus da Doença Hemorrágica Epizoótica/isolamento & purificação , Soros Imunes , Japão/epidemiologia , Tipagem Molecular , Testes de Neutralização , Filogeografia , Coelhos , Infecções por Reoviridae/epidemiologia , Infecções por Reoviridae/virologia , Sorogrupo
18.
J Vet Med Sci ; 79(5): 904-911, 2017 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-28392506

RESUMO

In September and October 2015, suspected cases of bovine ephemeral fever (BEF) were reported in the mainland region of Kagoshima Prefecture and on Tanegashima Island. The genome of the BEF virus (BEFV) was detected in the diseased cows and the cows that had recovered. The serum obtained from the affected cows contained high titers of BEFV-neutralizing antibody. In total, 18 affected cows were demonstrated to be infected with BEFV during the outbreak. Our findings showed evidence that BEF occurred in mainland Japan after a 23-year absence. Phylogenetic analysis based on the surface glycoprotein (G) gene revealed that BEFVs detected in the affected cows were genetically distinct from previous Japanese BEFVs, but were close to BEFVs circulating in Taiwan and mainland China in recent years. Amino acid substitution in the neutralizing epitope domains of the G protein was limited between the detected viruses and the vaccine strain (YHL isolate), and high titers of the neutralizing antibody against the YHL isolate were induced in the infected cattle during the disease occurrences. Therefore, current BEF vaccines probably elicit protective immunity against the BEFVs detected in 2015, although their effectiveness should be assessed. Since the BEFV vaccination rates are estimated to be low, a BEF outbreak should be considered a possibility in mainland Japan.


Assuntos
Febre Efêmera/diagnóstico , Animais , Anticorpos Neutralizantes/sangue , Anticorpos Antivirais/sangue , Bovinos , Surtos de Doenças/prevenção & controle , Surtos de Doenças/veterinária , Febre Efêmera/epidemiologia , Febre Efêmera/prevenção & controle , Febre Efêmera/virologia , Vírus da Febre Efêmera Bovina/classificação , Vírus da Febre Efêmera Bovina/genética , Vírus da Febre Efêmera Bovina/isolamento & purificação , Feminino , Insetos Vetores/virologia , Japão/epidemiologia , Filogenia , Vacinas Virais/administração & dosagem
19.
J Vet Med Sci ; 79(4): 774-779, 2017 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-28302930

RESUMO

We isolated an arbovirus from bovine blood in Indonesia. The arbovirus was obtained from the plasma of a cow showing no clinical symptoms in West Java in February 2014, and was identified as Akabane virus (AKAV) by AKAV-specific RT-PCR and subsequent sequence analysis. Phylogenetic analysis based on partial S segment indicated the AKAV isolate, WJ-1SA/P/2014, was most closely related with two isolates from Israel and Turkey reported in 2001 and 2015, respectively, and that WJ-1SA/P/2014 isolate belongs to AKAV genogroup Ib. This is the first isolation of AKAV from Indonesia.


Assuntos
Arbovírus/genética , Bovinos/virologia , Orthobunyavirus/genética , Animais , Arbovírus/classificação , Arbovírus/isolamento & purificação , Células Cultivadas , Indonésia , Orthobunyavirus/classificação , Orthobunyavirus/isolamento & purificação
20.
J Vet Med Sci ; 79(2): 277-281, 2017 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-27980290

RESUMO

A rare complex dysraphic malformation, comprising segmental spinal dysgenesis with caudal agenesis, was found in a Holstein calf that was unable to stand and was slightly short at the lumbosacral spine with taillessness. The thoracolumbar and sacrococcygeal regions of the midline axial segments showed severe deformities. In the spinal cord, the thoracolumbar region showed severe constriction with myelodysplastic changes, and the sacrococcygeal region showed dorsoventral separation with connection to a neural mass. In the spine, vertebral anomalies according to the degree of the segmentation error were confirmed. The cervical and thoracic segments also showed milder dysraphic changes. These changes suggest a multisegmental causal insult impairing the early embryonic notochord. This represents the first bovine case definitively confirmed morphologically.


Assuntos
Animais Recém-Nascidos/anormalidades , Bovinos/anormalidades , Coluna Vertebral/anormalidades , Animais , Vértebras Cervicais/anormalidades , Masculino , Medula Espinal/anormalidades , Vértebras Torácicas/anormalidades
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