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1.
Int Immunopharmacol ; 84: 106504, 2020 Apr 13.
Artigo em Inglês | MEDLINE | ID: mdl-32304994

RESUMO

AIM: To accumulate evidence that indicated the key role played by virus-triggered inflammation in the 2019-novel coronavirus disease (COVID-19) which emerged in Wuhan City and rapidly spread throughout China. METHODS: Age, neutrophil(NEU)-to-lymphocyte (LYM) ratio (NLR), lymphocyte-to-monocyte (MON) ratio, platelet-to-lymphocyte ratio (PLR), and C-reactive protein (CRP) of 93 patients with laboratory confirmed COVID-19 were investigated and compared. The receiver operating characteristic curve was applied to determine the thresholds for five bio-markers, and their prognostic values were assessed via the Kaplan-Meier curve and multivariate COX regression models. RESULTS: The median age was 46.4 years old, and 37cases were females. A total of 27.8% of patients had been to Wuhan, and 73.1% had contacted with people from Wuhan. Fever (83.8%) and cough (70.9%) were the two most common symptoms. Elevated NLR and age were significantly associated with illness severity. The binary logistic analysis identified elevated NLR (hazard risk [HR] 2.46, 95% confidence interval [CI] 1.98-4.57) and age (HR 2.52, 95% CI 1.65-4.83) as independent factors for poor clinical outcome of COVID-19. NLR exhibited the largest area under the curve at 0.841, with the highest specificity (63.6%) and sensitivity (88%). CONCLUSIONS: Elevated age and NLR can be considered independent biomarkers for indicating poor clinical outcomes.

2.
Org Lett ; 22(10): 3739-3743, 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-32186890

RESUMO

A novel 6/6/5/6 tetracyclic polyketide named chartspiroton (1) was isolated from a medicinal plant endophytic Streptomyces in Dendrobium officinale. The complete structure assignment with absolute stereochemistry was elucidated through spectroscopic data, computational calculations, and single-crystal X-ray diffraction. Chartspiroton features an unprecedented naphthoquinone derivative spiro-fused with a benzofuran lactone moiety. A plausible polyketide biosynthetic pathway for 1 suggested intriguing oxidative rearrangement steps to form the five-membered lactone ring.

3.
J Agric Food Chem ; 68(6): 1588-1595, 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-31994388

RESUMO

The discovery of new, safe, and effective pesticides is one of the main means for modern crop protection and parasitic disease control. During the search for new insecticidal secondary metabolites from endophytes in Stemona sessilifolia (a traditional Chinese medicine with a long history as an insecticide), 10 new insecticidal endostemonines A-J (1-10) were identified from an endophytic Streptomyces sp. BS-1. Their structures were determined by comprehensive spectroscopic analysis. Endostemonines A-J represent the first reported naturally occurring pyrrole-2-carboxylic ester derivatives, which consisted of different fatty acid chains at the C-2 of pyrrole ring were produced by traditional Chinese medicine endophytic microbes. All new tested compounds exhibited strong lethal activity against Aphis gossypii (LC50 value range of 3.55-32.00 mg/L after 72 h). This research highlighted the discovery of pesticide natural products from insecticidal medicinal plant endophytes for the first time, paving a new pathway for the development of pest control.


Assuntos
Endófitos/química , Compostos Heterocíclicos com 3 Anéis/metabolismo , Inseticidas/metabolismo , Stemonaceae/microbiologia , Streptomyces/química , Streptomyces/metabolismo , Animais , Afídeos/efeitos dos fármacos , Endófitos/metabolismo , Compostos Heterocíclicos com 3 Anéis/química , Compostos Heterocíclicos com 3 Anéis/toxicidade , Inseticidas/química , Inseticidas/toxicidade , Metabolismo Secundário
4.
Macromol Biosci ; 20(2): e1900303, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31867845

RESUMO

Self-healing hydrogels as wound dressings still face challenges in infection prevention, especially in the dressing of mass wounds, due to their inflexibility and the slow formation of the protective film on the wound. Therefore, designing a spray-filming (rapid-forming) hydrogel that can serve as a bacterial barrier is of particular significance in the development of wound dressings. Here, a self-healing hydrogel based on adipic acid dihydrazide-modified gelatin (Gel-ADH) and monoaldehyde-modified sodium alginate(SA-mCHO) is prepared. Using dynamic, Schiff base bonds, the hydrogels exhibit excellent self-healing properties. Moreover, the gelation time of SA-mCHO/Gel-ADH (SG) hydrogels is shortened to 2-21 s, resulting in rapid filming by spraying the two precursor solutions. In addition, the rapid spray-filming ability might offer sufficient flexibility and rapidity for dealing with mass and irregular wounds. Notably, the bacterial barrier experiments show that the SG hydrogel films could form an effective barrier to Staphylococcus aureus and Candida albicans for 12 h. Therefore, SG hydrogels could be used in wound dressings and they show great promise in applications associated with mass and irregular traumas.

5.
Colloids Surf B Biointerfaces ; : 110641, 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31759778

RESUMO

Rapid hemostasis is crucial to saving the lives of traumatic patients in emergency medical treatment. To improve the hemostatic performance of the gelatin microspheres (GMs) in vivo and in vitro, in the study, porous gelatin microspheres (PGMs) were prepared through water-in-oil emulsion method combined with vacuum freeze-drying after cross-linking with glutaraldehyde and prefreeze in liquid nitrogen. Owing to the porous structure and rough surface, the obtained PGMs were effective to induce red blood cells aggregation and promote fibrin generation, which led to improved hemostatic potential than GMs in blood coagulation time, whole blood clotting rate, and the hemostatic efficiency of rabbit liver wound and ear vein cut models tests. The potent hemostatic effect of the PGMs could be attributed to the synergistic effects of the physiological blood coagulation activated by negatively charged surface and physical barriers reinforced by fibrin. Moreover, PGMs with high water absorption rate and porous structure showed better hemostatic performance than commercial hemostatic powder (chitosan hemostasis power and Yunnan Baiyao) in vitro and in vivo. Cytotoxicity tests with bone marrow mesenchymal stem cells of murine showed that PGMs with excellent cytocompatibility were conducive to cell proliferation. Therefore, it could be concluded that PGMs were more suitable for rapid hemostasis than GMs and had a great potential for hemostatic applications.

6.
Opt Express ; 27(13): 18980-18987, 2019 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-31252832

RESUMO

Highly confined electromagnetic fields play a significant role in modern nano-optics, among which surface plasmon polaritons (SPPs) are outstanding because of their subwavelength and enhancement nature. While many state-of-the-art methods have been proposed to uncover the field distribution of SPPs, it still faces challenge to map the weak transverse field component (the field tangential to the interface) of SPPs with high contrast and precision. We propose a direct imaging technique, which employs a dielectric-nanoparticle-on-metal-film (DNP-MF) structure as a near-field probe, to overcome this difficulty. The angular distribution of the scattering radiation from the structure is strongly polarization dependent. By extracting the scattering signals that are mainly induced by the horizontal polarization, the imaging of the weak plasmonic transverse field with high precision can be achieved. The mappings of SPPs distributions excited by various vector beams were performed in experiment, which accord excellent with theory. This technique provides a new approach for near-field imaging with high contrast and reliability, which is expected to be valuable for studying the vectorial features of SPPs such as transverse spin, spin-orbit interactions, etc.

7.
Opt Express ; 27(6): 9250-9257, 2019 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-31052732

RESUMO

Structured illumination microscopy (SIM) is a powerful super-resolved imaging technique which enables to perform fast and in vivo imaging of bio-samples. In order to achieve a better resolution of a SIM system, evanescent waves with larger in-plane wave-vector are preferred for SIM, among which the total internal reflection (TIRF-SIM) and the plasmonic SIM (pSIM) configurations are widely studied. Here, we demonstrated a metal-dielectric waveguide (MDW) based SIM system - termed as MDW-SIM, which can achieve a good compromise between TIRF-SIM and pSIM. The MDW can support a low-loss waveguide mode at an aqueous environment, with an evanescent tail existing above the water/dielectric interface for SIM. A proof-of-concept imaging experiment was performed on fluorescent beads, where a spatial resolution of 86nm was achieved at a 473nm illumination wavelength and a 1.45 numerical aperture objective lens. The proposed MDW-SIM has a great potential for the bio-imaging applications.

8.
Gynecol Endocrinol ; 35(9): 777-781, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30982355

RESUMO

Objective: To investigate the MRI manifestations of congenital vaginal atresia, analyze its imaging features, and improve the understanding of the disease. Methods: MRI findings and clinical data of 12 patients with congenital vaginal atresia confirmed by hysteroscopy and laparoscopic surgery were retrospectively analyzed. Vaginal atresia was classified according to vaginal dysplasia in AFS female genital malformation classification system. Results: In this study, 12 cases of congenital vaginal atresia were diagnosed by combined preoperative MRI with operative diagnosis. Among them, 10 patients all had type-I congenital vaginal atresia, and their uterus and cervix were normal (1 patient had ectopic renal malformation combined with left ovarian endometriosis cyst and 1 patient with uterine empyema). The other two cases were diagnosed congenital vaginal atresia type II (1 case merged with residual uterus, 1 case with cervical dysplasia). MRI mainly manifested as dilatation and hemorrhage in the uterine cavity, cervical canal and vaginal upper segment. T1WI showed high signal, T2WI showed slightly lower and slightly higher signal. The dilated vagina was above the perineal level. Conclusion: MRI features of congenital vaginal atresia have certain characteristics. MRI cannot only accurately assess the type of vaginal dysplasia and its associated complications, but also make objective evaluation and diagnosis, so it can be used as the best effective preoperative image evaluation.


Assuntos
Imagem por Ressonância Magnética , Útero/anormalidades , Útero/diagnóstico por imagem , Doenças Vaginais/congênito , Doenças Vaginais/diagnóstico , Adolescente , Insuficiência Adrenal/diagnóstico , Adulto , Criança , Pré-Escolar , Anormalidades Congênitas/diagnóstico , Diagnóstico Diferencial , Feminino , Retardo do Crescimento Fetal/diagnóstico , Humanos , Lactente , Recém-Nascido , Osteocondrodisplasias/diagnóstico , Estudos Retrospectivos , Anormalidades Urogenitais/diagnóstico , Vagina/anormalidades , Adulto Jovem
9.
J Cell Biochem ; 2018 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-30317665

RESUMO

Previous studies have revealed that long noncoding RNA (lncRNA) and microRNA play a crucial role in autism, which is a childhood neurodevelopmental disorder with complicated genetic origins. Hence, the study concerns whether lncRNA C21orf121/bone morphogenetic proteins 2 (BMP2)/miR-140-5p gene network affects directed differentiation of stem cells from human exfoliated deciduous teeth (SHED) to neuronal cells in rats with autism. Autism models were successfully established. The neuron cells that differentiated from SHED cell were identified. The expression of lncRNA C21orf121, miR-140-5p, BMP2, Nestin, ßIII-tubulin, and microtubule-associated protein 2 (MAP2) and the expression of neuron-specific enolase (NSE) were examined. Besides, the gap junction (GJ) function of SHED, the intracellular free Ca 2+ concentration, and the social behavior and repetitive stereotyped movements of rats in autism were detected. The target relationship between lncRNA C21orf121 and miR-140-5p and that between miR-140-5p and BMP2 were also verified. Firstly, we successfully isolated SHED and identified the differentiated neurons of SHED. Besides, the expression of BMP2, MAP2, Nestin, ßIII-tubulin, NSE positive rate, GJ function, and intracellular free Ca 2+ concentration were increased with the upregulation of C21orf121 and downregulation of miR-140-5p, and accumulated time of repetitive stereotyped movements decreased and the frequency of social behavior increased. The results indicate that lncRNA C21orf121 as a competing endogenous RNA competes with BMP2 binding to miR-140-5p, thereby promoting SHED to differentiate into neuronal cells via upregulating BMP2 expression.

10.
Clin Interv Aging ; 13: 1313-1320, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30087557

RESUMO

Objective: Elderly people with mild cognitive impairment (MCI) are at high risk for dementia. This study compared the effects of standard cognitive training with a creative expression (CrExp) program. Methods: Adult patients with MCI aged 60 years and older (N=93) were randomly assigned to either CrExp therapy (n=48) or a control group who received standard cognitive training (n=45) for 16 weeks. The Montreal Cognitive Assessment, Chinese Version of the Auditory Verbal Learning Test, Chinese Version of the Category Verbal Fluency Test, Digit Span Test, Trail Making Test, Chinese Version of Activities of Daily Living scale, and Memory Satisfaction Questionnaire were used to measure cognitive functioning and daily living abilities. Assessments were administered at baseline, postintervention, and 6 months follow-up. Results: At postintervention, patients receiving CrExp therapy scored significantly higher than patients receiving standard cognitive training, in general cognitive functioning, memory, executive function, functional status, and everyday living ability. The improvements in cognitive functioning were maintained at the 6 month follow-up. Conclusion: CrExp therapy has greater positive effects on cognitive functions and daily living ability than standard cognitive training. This unique therapy may serve as a cost-effective adjunct to standard interventions for older adults with mild cognitive impairment.


Assuntos
Doença de Alzheimer/prevenção & controle , Terapia pela Arte/métodos , Disfunção Cognitiva/terapia , Atividades Cotidianas , Idoso , Idoso de 80 Anos ou mais , Cognição , Função Executiva , Feminino , Humanos , Masculino , Memória
11.
Rev. bras. farmacogn ; 28(2): 235-238, Mar.-Apr. 2018. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-1042261

RESUMO

ABSTRACT Twelve known compounds, including eight alkaloids, three lignans and one gossypol derivative, were isolated from the branches of Polyalthia rumphii (Blume ex Hensch.) Merr., Annonaceae. The chemical structures were determined by spectroscopic methods and comparison with literature data. All the isolates were evaluated the cytotoxicity against three human cancer cell lines: Hela, MCF-7 and A549, the results showed that partial of isolates displayed weak cytotoxicities with the IC50 values ranging from 25 to 40 µg/ml.

12.
Nord J Psychiatry ; 72(3): 179-183, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29216786

RESUMO

PURPOSE: Autism spectrum disorder (ASD) is a group of developmental brain disorders caused by genetic and environmental factors. The objective of this study was to investigate whether single nucleotide polymorphisms (SNPs) in genes related to immune function were associated with ASD in Chinese Han children. MATERIALS AND METHODS: A total of 201 children with ASD and 200 age- and gender-matched healthy controls were recruited from September 2012 to June 2106. A TaqMan probe-based approach was used to genotype SNPs corresponding to rs28532698 and rs4301112 in CD157, rs855867 in AIM2, and rs2237126 in JARID2. Case-control and case-only studies were performed to determine the contribution of SNPs to the predisposition of disease and its severity, respectively. RESULTS: Our results revealed that the genotypes and allele frequencies of these SNPs were not significantly associated with childhood ASD and its severity in this population. CONCLUSIONS: Results of our study suggest that these SNPs are not predictors of childhood ASD in the Chinese Han population. The discrepant results suggest the predictor roles of SNPs have to be determined in different ethnic populations due to genetic heterogeneity of ASD.


Assuntos
ADP-Ribosil Ciclase/genética , Antígenos CD/genética , Grupo com Ancestrais do Continente Asiático/genética , Transtorno do Espectro Autista/genética , Proteínas de Ligação a DNA/genética , Complexo Repressor Polycomb 2/genética , Polimorfismo de Nucleotídeo Único/genética , Adolescente , Grupo com Ancestrais do Continente Asiático/etnologia , Transtorno do Espectro Autista/diagnóstico , Transtorno do Espectro Autista/etnologia , Estudos de Casos e Controles , Criança , Pré-Escolar , Grupos Étnicos/genética , Feminino , Proteínas Ligadas por GPI/genética , Frequência do Gene , Predisposição Genética para Doença/etnologia , Predisposição Genética para Doença/genética , Genótipo , Humanos , Masculino
13.
J Mol Neurosci ; 62(2): 262-267, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28536923

RESUMO

Genetic variants have been implicated in the development of autism spectrum disorder (ASD). Recent studies suggest that solute carriers (SLCs) may play a role in the etiology of ASD. This purpose of this study was to determine the association between single nucleotide polymorphisms (SNPs) in SLC19A1 and SLC25A12 genes with childhood ASD in a Chinese Han population. A total of 201 autistic children and 200 age- and gender-matched healthy controls were recruited. A TaqMan probe-based real-time PCR approach was used to determine genotypes of SNPs corresponding to rs1023159 and rs1051266 in SLC19A1, and rs2056202 and rs2292813 in SLC25A12. Our results showed that both the T/T genotype of rs1051266 (odds ratio (OR) = 1.85, 95% confidence interval (CI) = 1.06-3.23, P = 0.0301) and the T allele (OR = 1.77, 95% CI = 1.07-2.90, P = 0.0249) of rs2292813 were significantly associated with an increased risk of childhood ASD. In addition, the G-C haplotype of rs1023159-rs1051266 in SCL19A1 (OR = 0.71, 95% CI = 0.51-0.98, P = 0.0389) and C-C haplotype of rs2056202-rs2292813 in SLC25A12 (OR = 0.58, 95% CI = 0.35-0.96, P = 0.0325) were associated with decreased risks of childhood ASD. There was no significant association between genotypes and allele frequencies with the severity of the disease. Our study suggests that these genetic variants of SLC19A1 and SLC25A12 may be associated with risks for childhood ASD.


Assuntos
Transtorno do Espectro Autista/genética , Polimorfismo de Nucleotídeo Único , Proteína Carregadora de Folato Reduzido/genética , Proteína Desacopladora 3/genética , Adolescente , Estudos de Casos e Controles , Criança , Pré-Escolar , China , Feminino , Frequência do Gene , Haplótipos , Humanos , Masculino
14.
Am J Transl Res ; 8(11): 4881-4891, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27904688

RESUMO

OBJECTIVE: To probe killing effect of busulfan to prostate cancer cell without androgen and the influence of androgen receptor phosphatization and analyze its molecular mechanism. METHODS: prostate cancer cell line 22RV1, LAPC4 and LNCaP treated with busulfan under androgen-free condition underwent CCK-8 examination to probe killing ability of the medicine. Flow cytometry was used to check the influence of busulfan on apoptosis rate of prostate cancer cell line LAPC4. Expression level of androgen receptor (AR), Src and Ack1 and change in phosphatization of AR after busulfan treatment were measured by RT-PCR and Western blotting. Finally, influence o proliferation ability and apoptosis of LAPC4 were measured using EGF-busulfan co-processing. RESULTS: Significant dose-dependency was observed as killing ability rises with higher busulfan concentration (p<0.05). Significant improvement in prostate cancer cell inhibition ability of busulfan was also observed with prolonging of time (p<0.05). Then we discovered, as indicated by flow cytometry, that busulfan inhibits prostate cancer cell LAPC4 proliferation by strengthening its apoptosis (p<0.05), which showed significant dose- and time-dependency. Detection of AR expression and phosphatization level showed no significant influence on mRNA and protein expression level of AR made by busulfan. However, decline of phosphatization level at AR Y534 site was positively related to busulfan treatment time. Busulfan was found to be inhibitory to Src kinase induced by EGF and level of resulting AR phosphatization in our further probe into the mechanism of busulfan influence on phosphatization level at AR Y534 site. Nude mice experiment indicated that busulfan was inhibitory to protein expression of AR downstream target gene prostate specific antigen (PSA) and human tissue kallikrein2 (hk-2), thus inhibited in vivo tumorigenic ability of prostate cancer cells. CONCLUSION: Busulfan was significantly inhibitory to prostate cancer cell proliferation by inhibiting phosphatization of Src kinase at AR Y534 site.

15.
J Child Neurol ; 31(5): 560-4, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26337060

RESUMO

Genetic factors play an important role in childhood autism. This study is to determine the association of single-nucleotide polymorphisms in dopa decarboxylase (DDC) and dopamine receptor-1 (DRD1) genes with childhood autism, in a Chinese Han population. A total of 211 autistic children and 250 age- and gender-matched healthy controls were recruited. The severity of disease was determined by Children Autism Rating Scale scores. TaqMan Probe by real-time polymerase chain reaction was used to determine genotypes and allele frequencies of single-nucleotide polymorphism rs6592961 in DDC and rs251937 in DRD1. Case-control and case-only studies were respectively performed, to determine the contribution of both single-nucleotide polymorphisms to the predisposition of disease and its severity. Our results showed that there was no significant association of the genotypes and allele frequencies of both single-nucleotide polymorphisms concerning childhood autism and its severity. More studies with larger samples are needed to corroborate their predicting roles.


Assuntos
Transtorno Autístico/genética , Dopa Descarboxilase/genética , Polimorfismo de Nucleotídeo Único/genética , Receptores de Dopamina D1/genética , Grupo com Ancestrais do Continente Asiático/etnologia , Grupo com Ancestrais do Continente Asiático/genética , Transtorno Autístico/etnologia , Criança , Pré-Escolar , Feminino , Frequência do Gene , Estudos de Associação Genética , Genótipo , Humanos , Masculino
16.
Clin Chem Lab Med ; 54(2): 345-51, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26351925

RESUMO

BACKGROUND: The aim of this study was to determine whether neutrophil CD64 (nCD64) combined with procalcitonin (PCT), C-reactive protein (CRP) and white blood cell count (WBC) can increase the sensitivity and accuracy of neonatal sepsis diagnosis. METHODS: The serum levels of nCD64, CRP, PCT and WBC were detected in 60 patients with neonatal sepsis and 60 patients with non-sepsis. Sensitivity, specificity, positive and negative predictive values, receiver operating characteristic (ROC) area under the curve (AUC), and logistic regression analysis were performed to evaluate the diagnostic value of these markers on neonatal sepsis. RESULTS: Serum levels of nCD64, PCT, CRP and WBC were higher in the sepsis group than non-sepsis group (p<0.001). The sensitivities of nCD64, PCT, CRP and WBC at the recommended cut-off level for all infants were 79.5%, 68.2%, 38.6% and 52.3%, respectively. The best combination was nCD64 and PCT, which obtained sensitivity of 90.9%, largest AUC of 0.922, and a negative predictive value of 89.2%. However by using an optimal cut-off value, the sensitivities of all four biomarkers for the diagnosis of neonatal sepsis were increased to 95.5%. Except for WBC, the birth weight and gestational age had no effects on the diagnostic value of these serum biomarkers. CONCLUSIONS: nCD64 and PCT are better diagnostic biomarkers for early diagnosis of neonatal sepsis as compared to CRP. With the help of optimal cut-off value based on ROC curve and logistic regression analysis, the combination of these biomarkers could improve the sensitivity for the diagnosis of suspected late-onset neonatal sepsis based on common serum biomarkers.


Assuntos
Proteína C-Reativa/análise , Calcitonina/sangue , Neutrófilos/metabolismo , Precursores de Proteínas/sangue , Receptores de IgG/sangue , Sepse/diagnóstico , Área Sob a Curva , Biomarcadores/sangue , Peso ao Nascer , Peptídeo Relacionado com Gene de Calcitonina , Estudos de Casos e Controles , Diagnóstico Precoce , Feminino , Idade Gestacional , Humanos , Lactente , Recém-Nascido , Contagem de Leucócitos , Modelos Logísticos , Masculino , Razão de Chances , Curva ROC , Estudos Retrospectivos , Sepse/sangue
17.
Opt Express ; 23(23): 29738-45, 2015 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-26698456

RESUMO

The hydrodynamic theory is a powerful tool to study the nonlocal effects in metallic nanostructures that are too small to obey classical electrodynamics while still too large to be handled with a full quantum-mechanical theory. The existing hydrodynamic model can give accurate quantitative predictions for the plasmonic resonance shifts in metallic nanoplasmonics, yet is not able to predict the spectral width which is usually taken as a pre-set value instead. By taking account the fact that due to electron density spill-out from a surface, the Coulomb interaction screening is less efficient close the surface thus leads to a higher electron-electron scattering rate in this paper, we study how the electron-density-related damping rate induced by such Coulomb interaction will affect the plasmonic spectral broadening. We perform the simulation on a Na nanowire, which shows that the absorption spectra width is wider when the size of the nanowire becomes smaller. This result is consistent well with the reported experiment. Therefore, our theoretical model extends the existing hydrodynamic model and can provide much more quantum insight about nonlocal effects in metallic nanostructures.

18.
Int J Nanomedicine ; 10: 6303-16, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26504382

RESUMO

OBJECTIVE: To evaluate the compatibility of novel nano-calcium-deficient hydroxyapatite/poly-amino acid (n-CDHA/PAA) complex biomaterials with muscle and bone tissue in an in vivo model. METHODS: Thirty-two New Zealand white rabbits were used in this study. Biomaterials were surgically implanted into each rabbit in the back erector spinae and in tibia with induced defect. Polyethylene was implanted into rabbits in the control group and n-CDHA/PAA into those of the experimental group. Animals were examined at four different points in time: 2 weeks, 4 weeks, 12 weeks, and 24 weeks after surgery. They were euthanized after embolization. Back erector spinae muscles with the surgical implants were examined after hematoxylin and eosin (HE) staining at these points in time. Tibia bones with the surgical implants were examined by X-ray and scanning electron microscopy (SEM) at these points in time to evaluate the interface of the bone with the implanted biomaterials. Bone tissues were sectioned and subjected to HE, Masson, and toluidine blue staining. RESULTS: HE staining of back erector spinae muscles at 4 weeks, 12 weeks, and 24 weeks after implantation of either n-CDHA/PAA or polyethylene showed disappearance of inflammation and normal arrangement in the peripheral tissue of implant biomaterials; no abnormal staining was observed. At 2 weeks after implantation, X-ray imaging of bone tissue samples in both experimental and control groups showed that the peripheral tissues of the implanted biomaterials were continuous and lacked bone osteolysis, absorption, necrosis, or osteomyelitis. The connection between implanted biomaterials and bone tissue was tight. The results of HE, Masson, toluidine blue staining and SEM confirmed that the implanted biomaterials were closely connected to the bone defect and that no rejection had taken place. The n-CDHA/PAA biomaterials induced differentiation of a large number of chondrocytes. New bone trabecula began to form at 4 weeks after implanting n-CDHA/PAA biomaterials, and lamellar bone gradually formed at 12 weeks and 24 weeks after implantation. Routine blood and kidney function tests showed no significant changes at 2 weeks and 24 weeks after implantation of both biomaterials. CONCLUSION: n-CDHA/PAA composites showed good compatibility in in vivo model. In this study, n-CDHA/PAA were found to be safe, nontoxic, and biologically active in bone repair.


Assuntos
Aminoácidos/química , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Durapatita/química , Teste de Materiais , Nanoestruturas/química , Polimerização , Animais , Cálcio/química , Diferenciação Celular/efeitos dos fármacos , Condrócitos/efeitos dos fármacos , Condrócitos/ultraestrutura , Masculino , Microscopia Eletrônica de Varredura , Músculos/citologia , Músculos/efeitos dos fármacos , Polietilenos/química , Próteses e Implantes , Coelhos , Tíbia/citologia , Tíbia/efeitos dos fármacos
19.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 27(8): 658-61, 2015 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-26255014

RESUMO

OBJECTIVE: To explore the role of parameters of organ function during heat stroke ( HS ) on the prognosis, and to form the treatment strategy through an analysis of parameters of organ function during HS. METHODS: A retrospective study was conducted. Thirty-seven patients with HS ( HS group ) and 54 patients with mild-to-moderate stroke ( stroke group ) admitted to Zhejiang Xiaoshan Hospital from 2011 to 2014 were enrolled. The experimental results of organs function indicators for patients were recorded including: (1) cardiac markers: troponin I ( TnI ); (2) myocardium zymogram: creatine kinase ( CK ), MB isoenzyme of creatine kinase ( CK-MB ), lactate dehydrogenase ( LDH ), and aspartate aminotransferase ( AST ); (3) renal function indexes: blood urea nitrogen ( BUN ), uric acid ( UA ), and serum creatinine ( SCr ); (4) electrolyte: serum K(+), Na(+), and Cl(-); (5)coagulation function: prothrombin time ( PT ), international normalized ratio ( INR ), activated partial thromboplastin time ( APTT ), thrombin time ( TT ), fibrinogen ( FIB ), and D-dimer; (6) blood gas analysis: pH value, arterial partial pressure of carbon dioxide ( PaCO(2)), base excess ( BE ), standard bicarbonate ( SB ), and actual bicarbonate ( AB ); (7) routine blood test: blood platelet count ( PLT ); (8) hepatic function: alanine aminotransferase ( ALT ). Abnormal rates of laboratory parameters of 37 HS patients were statistically analyzed. Various laboratory parameters of organs function as well as the initial value and extreme value ( maximum or minimum value ) during treatment of CK and PLT in HS patients were compared between two groups. RESULTS: The abnormal rates of 37 HS patients were more than 70%, including incipient value of TnI, CK, LDH, AST, serum Na(+), ALT, D-dimer, PaCO(2), AB, maximum value of CK, and minimum value of PLT, the abnormal rates being 73.0%, 70.3%, 81.1%, 78.4%, 78.4%, 70.3%, 70.3%, 70.3%, 75.7%, 81.1%, 75.7%, respectively. The abnormal rates of other parameters were less than 70%. There were significant differences in incipient value of TnI, CK, LDH, AST, serum K(+), serum Na(+), D-dimer, and PLT between HS group and mild-to-moderate stroke group [ TnI ( µg/L ): 0.087 ( 0.026, 0.306 ) vs. 0.007 ( 0.004, 0.110 ), Z = -7.017, P = 0.000; CK ( U/L ): 392.30 ( 287.60, 524.10 ) vs. 137.10 ( 106.33, 607.80 ), Z = -7.930, P = 0.000; LDH ( U/L ): 317.98±122.74 vs. 207.85±57.71, t = 1.678, P = 0.000; AST ( U/L ): 94.90 ( 52.80, 155.80 ) vs. 26.10 ( 18.13, 317.40 ), Z = -6.157, P = 0.000; serum K(+) ( mmol/L ): 3.46±0.65 vs. 3.86±0.57, t = 1.662, P = 0.001; serum Na(+) ( mmol/L ): 129.75±7.34 vs. 138.79±4.26, t = 1.674, P = 0.000; D-dimer ( mg/L ): 2.53 ( 0.63, 6.00 ) vs. 0.30 ( 0.21, 9.71 ), Z = -5.084, P = 0.000; PLT ( ×10(9)/L ): 144.62±86.14 vs. 219.48±64.76, t = 1.669, P = 0.000 ]. There were also statistically significant differences in the initial value and extreme value of CK and PLT between HS group and mild-to-moderate stroke group [ CK ( U/L ): 392.30 ( 287.60, 524.10 ) vs. 721.50 ( 546.30, 964.10 ), Z = -6.351, P = 0.000; PLT ( ×10(9)/L ): 132.40±82.55 vs. 68.24±44.62, t = 1.688, P = 0.000 ]. CONCLUSIONS: HS can impair several organs and systems, having complications, and it is a heavy insult for body. Increasing of CK and decreasing of PLT has some value to assess illness changes. It is helpful of laboratory results for doctors to estimate complications on time.


Assuntos
Biomarcadores/análise , Técnicas de Laboratório Clínico/métodos , Golpe de Calor/diagnóstico , Golpe de Calor/terapia , Alanina Transaminase/análise , Testes de Coagulação Sanguínea , Gasometria , Nitrogênio da Ureia Sanguínea , Creatina Quinase Forma MB/análise , Eletrólitos/análise , Produtos de Degradação da Fibrina e do Fibrinogênio/análise , Humanos , Testes de Função Renal , Tempo de Tromboplastina Parcial , Contagem de Plaquetas , Prognóstico , Tempo de Protrombina , Estudos Retrospectivos , Troponina I
20.
Am J Med Genet B Neuropsychiatr Genet ; 168B(4): 236-46, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25921325

RESUMO

The solute carrier family 25 (aspartate/glutamate carrier), member 12 gene (SLC25A12) has been strongly posed as a candidate gene for autism spectrum disorder (ASD) given its important role in mitochondrial function and adenosine triphosphate (ATP) synthesis. Evidence is mounting for the association between SLC25A12 variants (rs2056202 and rs2292813) and ASD risk, but the results are inconsistent. To clarify the effect of these two variants on ASD, a meta-analysis integrating case-control and transmission disequilibrium test (TDT) studies was performed. The PubMed, Embase, Cochrane Library, Web of Science, Chinese BioMedical Literature (CBM), Wanfang, and Chinese National Knowledge Infrastructure (CNKI) databases were systematically searched to identify relevant studies published up to May 2014. Odds ratios (ORs) and 95% confidence intervals (95%CIs) were calculated to assess the strength of association. A total of 775 cases, 922 controls, and 1289 families available from 8 studies concerning rs2056202, and 465 cases, 450 controls, and 1516 families available from 7 studies concerning rs2292813 were finally included. In the overall meta-analysis, the rs2056202 T allele and rs2292813 T allele were both significantly associated with a decreased risk of ASD (rs2056202: OR = 0.809, P = 0.001, 95%CI: 0.713-0.917, I(2) = 0.0%, and P(heterogeneity) = 0.526; rs2292813: OR = 0.752, P < 0.001, 95%CI: 0.649-0.871, I(2) = 0.0%, P(heterogeneity) = 0.486). Besides, subjects with T-T haplotype of rs2056202-rs2292813 had a significantly reduced risk of ASD (OR = 0.672, P < 0.001, 95%CI: 0.564-0.801, I(2) = 0.0%, P(heterogeneity) = 0.631). Sensitivity analysis, cumulative meta-analysis, and publication bias diagnostics confirmed the reliability and stability of our results. Our meta-analysis suggests that rs2056202 and rs2292813 in SLC25A12 may contribute significantly to ASD risk.


Assuntos
Transtorno do Espectro Autista/genética , Estudos de Associação Genética , Predisposição Genética para Doença , Proteínas de Transporte da Membrana Mitocondrial/genética , Polimorfismo de Nucleotídeo Único/genética , Heterogeneidade Genética , Haplótipos/genética , Humanos , Viés de Publicação , Fatores de Risco
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