Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 232
Filtrar
1.
RSC Adv ; 13(3): 1606-1616, 2023 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-36688063

RESUMO

Xanthine oxidase (XO) is a key target for gout treatment. Great efforts have been made towards the discovery and development of new XO inhibitors. Aureusidin (AUR), a natural compound, emerges as the second reported XO inhibitor with an aurone skeleton with an IC50 value of 7.617 ± 0.401 µM in vitro. The inhibitory mechanism of AUR against XO was explored through enzyme kinetic studies, multi-spectroscopic methods, computer simulation techniques, and ADME prediction. The results showed that AUR acts as a rapid reversible and mixed-type XO inhibitor and its binding to XO was driven by hydrogen bonding and hydrophobic interaction. Moreover, AUR presented a strong fluorescence quenching effect through a static quenching process and induced a conformation change of XO. Its binding pattern with XO was revealed through molecular docking, and its affinity toward XO was enhanced through interactions with key amino acid residues in the active pocket of XO. Further, AUR demonstrated good stability and pharmacokinetic behavior properties in molecular dynamics simulation and ADME prediction. In short, the current work clarified in depth the inhibitory mechanism of AUR on XO firstly and then provided fresh insights into its further development as a natural potent XO inhibitor with aurone skeleton.

2.
Angew Chem Int Ed Engl ; : e202216340, 2023 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-36591914

RESUMO

Side-chain tailoring is a promising method to optimize the performance of organic solar cells (OSCs). However, asymmetric alkyl chain-based small molecular acceptors (SMAs) are still difficult to afford. Herein, we adopted a novel asymmetric n-nonyl/undecyl substitution strategy and synthesized two A-D1 A'D2 -A double asymmetric isomeric SMAs with asymmetric selenophene-based central core for OSCs. Crystallographic analysis indicates that AYT9Se11-Cl forms a more compact and order intermolecular packing compared to AYT11Se9-Cl, which contributed to higher electron mobility in neat AYT9Se11-Cl film. Moreover, the PM6 : AYT9Se11-Cl blend film shows a better morphology with appropriate phase separation and distinct face-on orientation than PM6 : AYT11Se9-Cl. The OSCs with PM6 : AYT9Se11-Cl obtain a superior PCE of 18.12 % compared to PM6 : AYT11Se9-Cl (17.52 %), which is the best efficiency for the selenium-incorporated SMAs in binary BHJ OSCs. Our findings elucidate that the promising double asymmetric strategy with isomeric alkyl chains precisely modulates the crystal packing and enhances the photovoltaic efficiency of selenophene-incorporated SMAs.

3.
Clin Chim Acta ; 540: 117227, 2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36640930

RESUMO

BACKGROUND: Early stratification of disease progression remains one of the major challenges towards the post-coronavirus disease 2019 (COVID-19) era. The clinical relevance of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid load is debated due to the heterogeneity in patients' underlying health conditions. We determined the prognostic value of nasopharyngeal viral load dynamic conversion for COVID-19. METHODS: The cycling threshold (Ct) values of 28,937 nasopharyngeal SARS-CoV-2 RT-PCRs were retrospectively collected from 3,364 COVID-19 patients during hospitalization and coordinated to the onset of disease progression. The ROC curve was utilized to determine the predictive performance of the rate of Ct value alteration between two consecutive RT-PCR runs within 48 h (ΔCt%) for disease transformation across patients with different COVID-19 severity and immune backgrounds, and further validated with 1,860 SARS-CoV-2 RT-PCR results from an independent validation cohort of 262 patients. For the 67 patients with severe COVID-19, Kaplan-Meier analysis was performed to evaluate the difference in survival between patients stratified by the magnitude of Ct value alteration between the late and early stages of hospitalization. RESULTS: The kinetics of viral nucleic acid conversion diversified across COVID-19 patients with different clinical characteristics and disease severities. The ΔCt% is a clinical characteristic- and host immune status-independent indicator for COVID-19 progression prediction (AUC = 0.79, 95 % CI = 0.76 to 0.81), which outperformed the canonical blood test markers, including c-reactive protein (AUC = 0.57, 95 % CI = 0.53 to 0.61), serum amyloid A (AUC = 0.61, 95 % CI = 0.54 to 0.68), lactate dehydrogenase (AUC = 0.61, 95 % CI = 0.56 to 0.67), d-dimer (AUC = 0.56, 95 % CI = 0.46 to 0.66), and lymphocyte count (AUC = 0.62, 95 % CI = 0.58 to 0.66). Patients with persistent high SARS-CoV-2 viral load (an increase of mean Ct value < 50 %) during the first 3 days of hospitalization demonstrated a significantly unfavorable survival (HR = 0.16, 95 % CI = 0.04 to 0.65, P = 2.41 × 10-3). CONCLUSIONS: Viral nucleic acid dynamics of SARS-CoV-2 eliminates the inter-patient variance of basic health conditions and therefore, can serve as a prognostic marker for COVID-19.

4.
J Affect Disord ; 322: 63-75, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36372121

RESUMO

BACKGROUND: The mechanism by which synaptic plasticity mediates the occurrence of depression is unknown. Low-density lipoprotein receptor-related protein 1 (LRP1) affects axon growth and neurogenesis in the brain, but its role in depressive-like behaviors is poorly understood. METHODS: Adeno-associated virus-mediated small interfering RNA was injected into the bilateral hippocampus 14 days before chronic unpredicted mild stress (CUMS). Behavior performance was assessed for depressive-like behaviors. Western blot was conducted to detect levels of LRP1, neurogenesis-related proteins, synaptic markers, microtubule system molecules and Akt/GSK-3ß signaling-related proteins. Immunohistochemical staining was performed for LRP1 protein, immunofluorescence staining was conducted to determine the Sox2 protein, Nissl's staining and transmission electron microscope staining were used to observe hippocampal morphological features. RESULTS: The expression of hippocampal LRP1 was positively correlated with depressive-like behaviors. Treatment with iAAV-LRP1 exerted protective effects on depressive-like behaviors. LRP1 Knockdown relieved the inhibition of synaptic plasticity induced by CUMS. Expression of Sox2, GluR2 and SYP was significantly increased in iAAV-LRP1 CUMS rats. LRP1 knockdown reduced the p-tau (Ser262 and Thr404) and Acet-tubule levels in depressed rats. Finally, we found that LRP1 knockdown activated the PI3K/Akt pathway and inhibited GSK-3ß signal transduction. LIMITATIONS: More neurogenesis markers would be considered, and stereotactic injection into hippocampal DG region could be performed to investigate the effects of LRP1. CONCLUSIONS: These findings indicated that hippocampal LRP1 deficiency in stressed rats plays an important protective role in depressive-like behavior by increasing synaptic plasticity mediated by microtubule dynamic and activating Akt/GSK-3ß signaling pathway. Therefore, LRP1 may represent a potential therapeutic target for depression.


Assuntos
Fosfatidilinositol 3-Quinases , Proteínas Proto-Oncogênicas c-akt , Animais , Ratos , Glicogênio Sintase Quinase 3 beta/metabolismo , Hipocampo/metabolismo , Microtúbulos/metabolismo , Plasticidade Neuronal/fisiologia , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Estresse Psicológico/complicações , Estresse Psicológico/metabolismo
5.
Bioorg Chem ; 131: 106320, 2023 02.
Artigo em Inglês | MEDLINE | ID: mdl-36527991

RESUMO

Xanthine oxidase (XO) is a crucial target for the treatment of hyperuricemia and gout. A series of derivatives based on natural 3,4-dihydroxychalcone, obtained from Carthamus tinctorious and Licorice, were designed and synthesized. Nine derivatives (9a-e, 10b,c, and 15a,b) exhibited apparent XO inhibitory activity in vitro (IC50 values varied from 0.121 to 7.086 µM), 15b presented the most potent inhibitory activity (IC50 = 0.121 µM), which was 27.47-fold higher than that of allopurinol (IC50 = 3.324 µM). The SAR analysis indicated that introducing hydroxyl groups at 3'/4'/5'-position on ring A was more beneficial to the inhibition of XO than at 2'/6'-position; the removal of 3­hydroxyl group on ring B could weaken the inhibitory potency of hydroxychalcones on XO, but it was beneficial to the XO inhibitory potency of methoxychalcones. Molecule modeling studies afforded insights into the binding mode of 15b with XO and supported the findings of SAR analysis. Additionally, kinetics studies demonstrated that 15b presented a reversible and competitive XO inhibitor, which spontaneously combined with XO through hydrophobic force, and finally changed the secondary conformation of XO. Furthermore, the acute hyperuricemia model was employed to investigate the hypouricemic effect of 15b, which could effectively reduce the serum uric acid levels of rats at an oral dose of 10 mg/kg. ADMET prediction suggested that compound 15b possessed good pharmacokinetic properties. Briefly, compound 15b emerges as an interesting XO inhibitor for the treatment of hyperuricemia and gout with beneficial effects on serum uric acid levels regulating. Meanwhile, the XO inhibitors with chalcone skeleton will deserve further attention and discussion.


Assuntos
Chalcona , Chalconas , Gota , Hiperuricemia , Ratos , Animais , Relação Estrutura-Atividade , Ácido Úrico , Chalconas/farmacologia , Chalconas/uso terapêutico , Xantina Oxidase , Chalcona/farmacologia , Chalcona/uso terapêutico , Hiperuricemia/tratamento farmacológico , Hiperuricemia/metabolismo , Inibidores Enzimáticos/química , Gota/tratamento farmacológico
6.
BMC Sports Sci Med Rehabil ; 14(1): 208, 2022 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-36494746

RESUMO

BACKGROUND: Due to the COVID-19 pandemic, the 2020 season Chinese Super League (CSL) was held in neutral venues, this study aims to analyse the impact of removing home advantage (HA) in CSL. METHOD: 240 games of the CSL 2019 season (home and away double round-robin system) and 160 games of the 2020 season (in neutral venues) were analysed. 27 technical and tactical performance indicators were involved as dependent variables. A multiple linear regression model was established to analyse the influence of removing HA on the performance indicators. RESULTS: After moving from home stadium to neutral venue in 2020 season, goal, shot, shot on target, shot from outside box, shot from inside box, shot on target from inside box, corner kick, key pass, cross, breakthrough, tackle decreased significantly (p < 0.05), while yellow card and foul increased steeply (p < 0.05). Comparing with playing away match, in neutral venue, free kicks and pass accuracy enhanced radically (p < 0.05), while tackle, clearance and block shot dropped noticeably (p < 0.05). CONCLUSION: When removing HA and playing in the neutral venue, teams' performance dropped significantly. This study confirmed the positive impact of HA on the teams' performance and may help elite football teams make proper playing strategies regarding different match locations.

7.
J Adv Res ; 2022 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-36496174

RESUMO

INTRODUCTION: Flavor is a major contributor to consumer preference. Despite being effective at extending the fruit's commercial life, cold storage also results in a significant loss of flavor volatiles. To date, there has been few studies on the metabolic dynamics and the mechanism underlying the regulatory networks that modulate flavor loss during cold storage for fruit. METHODS: The volatile contents were detected by Gas Chromatography-Mass Spectrometer (GC-MS). Weighted gene co-expression network analysis (WGCNA) was used to identify structure genes and transcription factors (TFs). DNA methylation was analyzed by whole-genome methylation sequencing during cold storage. RESULTS: We generated a temporal map, over hourly to weekly timescales, for the effects of chilling on flavor volatiles by combining metabolome, transcriptome, and DNA methylome in peach fruit. Based on the big data analysis, we developed a regulatory network for volatile formation and found that a decrease in volatiles during cold storage was significantly correlated with a decrease in the expression of synthesis genes. Moreover, TFs associated with these structure genes were identified. Expression of genes involved in ethylene biosynthesis was reduced while cold tolerance pathway was activated in response to low temperature. Functions of those genes were confirmed through transgenic experiments and across peach cultivars, suggesting our dataset is a useful tool for elucidating regulatory factors that have not yet been clarified in relation to flavor and cold tolerance. Genome wide DNA methylation was induced by chilling and peaked at 7 d followed by a decline during 28 d cold storage. Reduction of gene expression was accompanied by major changes in the methylation status of their promoters, including PpACS1, PpAAT1, PpTPS3 and PpMADS2. CONCLUSION: Our study revealed the mechanism for chilling-induced flavor loss of peach fruit through time-course transcriptome and DNA methylome analysis.

8.
J Psychiatr Res ; 156: 698-704, 2022 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-36410308

RESUMO

Air pollution is a risk factor for increased hospital admissions due to mental disorders, while green spaces have been linked with better mental health. We linked daily hospital admission records from Wuhan's 74 municipal hospitals from 2017 to 2019 with modeled annual average NO2 concentrations and added data on the residential surrounding green spaces with 250 m and 500 m buffers based on the normalized difference vegetation index (NDVI) using a land use regression model (LUR). The conditional logistic regression model was used to estimate the acute effect of short-term NO2 exposure, and stratification analyses were applied to explore the modification effect of long-term NO2 exposure and green spaces by estimating the odds ratios in the single- and dual-environmental factor groups. A total of 42,705 hospital admissions for mental disorders were identified. Short-term exposure to NO2 was associated with an increased risk of hospital admission for mental disorders. A 10 µg/m3 increase in NO2 (lag01 day) was associated with an increase in hospital admissions of 2.86% (95% CI, 2.05-3.68) for the total mental disorders. Compared with patients in the "low-NDVI/low-NO2" group (ER = 2.27%, 95% CI, 0.27-4.31), patients in the "high-NDVI/low-NO2" group (ER = 1.93%, -0.10-3.99) showed a lower and insignificant increase in hospitalizations for the total mental disorders, while greenness had a slight moderating effect in the high-level long-term NO2 exposure areas. This study suggested that green spaces may moderate the acute effect of NO2 exposure for mental disorder hospitalizations, especially in low-level long-term NO2 exposure areas.

9.
BMC Neurosci ; 23(1): 67, 2022 11 19.
Artigo em Inglês | MEDLINE | ID: mdl-36401162

RESUMO

BACKGROUND: This study aimed to investigate whether CXCL1/CXCR2 mediates intestinal injury or white matter injury by delivering inflammatory mediators through the gut-brain regulation axis. METHODS: Neonatal SD rats, regardless of sex, were administered 3% dextran sulfate sodium via intragastric administration at different time points to construct necrotizing enterocolitis (NEC) models. Meanwhile, hypoxia and ischemia were induced in 3 day-old SD rats to construct hypoxic-ischemic brain injury (HIBI) and NEC + HIBI models, without gender discrimination. Hematoxylin-eosin staining was used to observe pathological changes in neonatal rat intestinal and brain tissues. Western blotting detected CXCL1 and CXCR2 expression in NEC, HIBI, and NEC + HIBI rat intestinal and brain tissues. RESULTS: Compared with normal rats, pathological damage to periventricular white matter was observed in the NEC group. In addition to the increased mortality, the histopathological scores also indicated significant increases in brain and intestinal tissue damage in both HIBI and NEC + HIBI rats. Western blotting results suggested that CXCL1 and CXCR2 expression levels were upregulated to varying degrees in the intestinal and brain tissues of NEC, HIBI, and NEC + HIBI neonatal rats compared to that in the normal group. Compared with the HIBI group, the expression of CXCL1 and CXCR2 continued to increase in NEC + HIBI rats at different time points. CONCLUSIONS: CXCL1/CXCR2 may be involved in white matter injury in neonatal rats by delivering intestinal inflammatory mediators through the gut-brain axis.


Assuntos
Lesões Encefálicas , Enterocolite Necrosante , Hipóxia-Isquemia Encefálica , Substância Branca , Animais , Ratos , Animais Recém-Nascidos , Ratos Sprague-Dawley , Eixo Encéfalo-Intestino , Modelos Animais de Doenças , Hipóxia-Isquemia Encefálica/metabolismo , Enterocolite Necrosante/metabolismo , Quimiocina CXCL1/metabolismo
10.
Allergol Immunopathol (Madr) ; 50(6): 115-121, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36335454

RESUMO

BACKGROUND: Psoriasis is considered as an inflammatory skin disease accompanied by dyslipidemia comorbidity. B-cell leukemia-3 (Bcl-3) belongs to IκB (inhibitor of nuclear factor kappa B [NF-κB]) family, and regulates inflammatory response through associating with NF-κB. The role of Bcl-3 in psoriasis was investigated in this study. METHODS: Apolipoprotein E (ApoE)-deficient mice were treated with imiquimod to induce psoriasis and dyslipidemia. Mice were injected intradermally in the back with lentiviral particles encoding Bcl-3 small hairpin RNA (shRNA). Hematoxylin and eosin were used to detect pathological characteristics. The blood lipid levels were determined by automatic biochemical analyzer, and inflammation was assessed by enzyme-linked-immunosorbent serologic assay and real-time quantitative reverse transcription polymerase chain reaction. RESULTS: Bcl-3 was elevated in imiquimod-induced ApoE-deficient mice. Injection with lentiviral particles encoding Bcl-3 shRNA reduced Psoriasis area and severity index (PASI) score in ApoE-deficient psoriatic mice. Knockdown of Bcl-3 also ameliorated imiquimod-induced psoriasiform skin lesions in ApoE-deficient mice. Moreover, loss of Bcl-3 enhanced expression of loricrin, an epidermal barrier protein, reduced expression of proliferating cell nuclear antigen (PCNA) and lectin-like oxidized LDL (oxLDL) receptor-1 (LOX-1) in imiquimod-induced ApoE-deficient mice. The enhanced levels of blood lipid in ApoE-deficient mice were attenuated by silencing of Bcl-3 with increase of high-density lipoprotein, and reduction of total cholesterol, triglycerides, and low-density lipoprotein cholesterol. Knockdown of Bcl-3 attenuated imiquimod-induced decrease of transforming growth factor beta (TGF-ß), and increase of Interleukin (IL)-17A, IL-23, IL-6, and tumor necrosis factor-α (TNF-α) in ApoE-deficient mice. Protein expression of phospho-Akt (p-Akt) and p-GSK3ß in ApoE-deficient psoriatic mice was decreased by silencing of Bcl-3. CONCLUSION: Loss of Bcl-3 exerted anti-inflammatory effect on psoriasis and dyslipidemia comorbidity through inactivation of Akt/GSK3ß pathway.


Assuntos
Dislipidemias , Leucemia de Células B , Psoríase , Camundongos , Animais , Imiquimode/efeitos adversos , Imiquimode/metabolismo , NF-kappa B/metabolismo , Proteínas Proto-Oncogênicas c-akt/metabolismo , Glicogênio Sintase Quinase 3 beta/metabolismo , Glicogênio Sintase Quinase 3 beta/farmacologia , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/efeitos adversos , RNA Interferente Pequeno/metabolismo , Psoríase/tratamento farmacológico , Psoríase/metabolismo , Comorbidade , Colesterol , Leucemia de Células B/metabolismo , Leucemia de Células B/patologia , Apolipoproteínas E/efeitos adversos , Apolipoproteínas E/metabolismo , Camundongos Endogâmicos BALB C , Modelos Animais de Doenças , Pele/patologia
11.
Horm Metab Res ; 2022 Nov 29.
Artigo em Inglês | MEDLINE | ID: mdl-36446568

RESUMO

The purpose of this study is to explore the predictive value of cytokine levels in the first trimester of pregnancy on abnormal liver function of pregnant women with hepatitis B in the third trimester of pregnancy. A total of 111 pregnant women with HBV infection at 12 weeks gestation participated in the study. The levels of IL-6, IL-8, TNF-α in peripheral blood of the patients and liver function indexes were detected. Subsequently, the pregnant women were followed up, and the liver function was detected at 36 weeks of gestation. According to liver function indexes, patients were divided into normal liver function group and abnormal liver function group to determine the correlation between cytokines in early pregnancy and abnormal liver function in late pregnancy. Kaplan-Meier survival curve and multivariate Cox analysis were used to evaluate the predictive value of cytokines for liver dysfunction. At 12 weeks of gestation, cytokine levels in the normal liver function group were significantly lower than that in the abnormal liver function group. Kaplan-Meier survival analysis showed that the increased IL-6 level was associated with abnormal liver function in late pregnancy. Multivariate Cox regression analysis revealed that IL-6 level was an independent predictor of abnormal liver function in patients with normal liver function in the late pregnancy. The high expression level of cytokine IL-6 at 12 weeks of pregnancy has noteworthy predictive significance for the abnormal liver function of hepatitis B pregnant women in third trimester of pregnancy.

12.
J Insect Sci ; 22(5)2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-36268784

RESUMO

Arma custos (Fabricius) (Hemiptera: Pentatomidae) is a natural predator that can control various agricultural and forestry pests. This study aimed to clarify the effects of temperature on the growth, reproduction, and population of the predator and to simulate its population growth. Using the age-stage, two-sex life table method, 18°C, 22°C, 26°C, 30°C, and 34°C were selected as the temperature conditions. A. custos can complete its life cycle at 18°C-30°C, and the developmental duration of each A. custos stage, adult pre-oviposition period, total pre-oviposition period, and the mean generation time (T) were shortened with the increase in temperature. The pre-adult mortality was significantly reduced at 26°C and 30°C. In addition, the fecundity of a single female and the gross reproductive rate were the highest at 30°C. Significant differences were observed in the intrinsic rate of increase (r) and the finite rate of increase (λ) under different temperature conditions, and both reached the maximum at 30°C. Results showed that adult A. custos raised at 26°C had a longer lifespan and the fecundity was higher at 30°C in comparison with the other temperatures. This study is the first to report the life cycle of A. custos at different temperatures, and the results can provide a scientific theoretical basis for the indoor artificial reproduction, outdoor release, and colonization of A. custos.


Assuntos
Hemípteros , Heterópteros , Feminino , Animais , Temperatura , Crescimento Demográfico , Reprodução , Fertilidade , Estágios do Ciclo de Vida
13.
Opt Express ; 30(20): 36603-36621, 2022 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-36258585

RESUMO

In order to meet the needs of multi-spectral radiation temperature measurement under high temperature background, this paper studies the problems of reflected radiation interference and spectral emissivity difficult to obtain in high temperature and intense reflection environment. First, using discrete triangular surface elements and radiation angle coefficients, an analysis model of high temperature background reflected radiation is constructed to describe the variation characteristics of high temperature background reflected radiation. Secondly, the least squares support vector machine (LSSVM) is optimized by particle swarm optimization (PSO) algorithm, and an emissivity model identification algorithm based on Alpha spectrum-Levenberg Marquarelt (LM) algorithm is proposed, which has stronger applicability and accuracy than existing emissivity model identification methods. Finally, the high temperature background radiation and the emissivity model are combined to construct and solve the multi-spectral target equation, so as to realize the reflected radiation error correction and radiation temperature measurement under the high temperature and intense reflection background. The simulation and experimental comparison with the existing methods show that the temperature measurement error of the radiation temperature measurement method proposed in this paper is below 9.5K, which can effectively correct the reflected radiation error and further improve the temperature measurement accuracy.

14.
Front Psychol ; 13: 947108, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36092077

RESUMO

Under the background of green development, multidimensional R&D investment and institutional quality have injected strong power into green innovation. Based on China's provincial panel data from 2009 to 2018, this study examines the threshold effect of R&D and R&D personnel input on China's green innovation capability from three perspectives, namely, political institutional quality, economic institutional quality, and legal institutional quality. The core study results show that the influence of R&D on China's green innovation capability has an obvious double-threshold effect based on institutional quality. This study expands the research on the influencing factors of green innovation and the influence effect of multidimensional R&D investment and provides a theoretical basis for regional green innovation management. In addition, the research results of this study provide a reference for accurately formulating regional green innovation capability promotion strategies.

15.
Front Psychol ; 13: 933134, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36092100

RESUMO

This study examines the impact of digital financing on the degree of financing constraints and discusses the mediating effect of investor confidence. The data are based on companies listed on the Shanghai Stock Exchange and the Shenzhen Stock Exchange from 2010 to 2019. To investigate the impact of digital financing on the financing constraints of companies in different situations, the heterogeneity of internal control and equity characteristics of different organizations is analyzed. The results using fixed-effects models show that (i) the change in digital finance has a significant negative impact on the level of corporate financing constraints; (ii) investor confidence plays a mediating role between digital finance and financing constraints; and (iii) the level of internal control impacts the relationship between the digital finance and the corporate financing constraints. Specifically, for the organizations with better internal control, there is a significant negative relationship between digital finance and corporate financing constraints while for organizations with poor internal control, digital finance has no significant influence on the extent of financing constraints; and (iv) digital finance of private organizations is significantly negatively correlated with the extent of financing constraints, while for government organizations, a negative relationship is not evident.

16.
Int J Mol Sci ; 23(18)2022 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-36142414

RESUMO

Calmodulin-binding transcription activator (CAMTA) is a transcription factor family containing calmodulin (CaM) binding sites and is involved in plant development. Although CAMTAs in Arabidopsis have been extensively investigated, the functions of CAMTAs remain largely unclear in peaches. In this study, we identified five peach CAMTAs which contained conserved CG-1 box, ANK repeats, CaM binding domain (CaMBD) and IQ motifs. Overexpression in tobacco showed that PpCAMTA1/2/3 were located in the nucleus, while PpCAMTA4 and PpCAMTA5 were located in the plasma membrane. Increased expression levels were observed for PpCAMTA1 and PpCAMTA3 during peach fruit ripening. Expression of PpCAMTA1 was induced by cold treatment and was inhibited by ultraviolet B irradiation (UV-B). Driven by AtCAMTA3 promoter, PpCAMTA1/2/3 were overexpressed in Arabidopsis mutant. Here, we characterized peach PpCAMTA1, representing an ortholog of AtCAMTA3.&nbsp;PpCAMTA1 expression in Arabidopsis complements the developmental deficiencies of the camta2,3 mutant, and restored the plant size to the wild type level. Moreover, overexpressing PpCAMTA1 in camta2,3 mutant inhibited salicylic acid (SA) biosynthesis and expression of SA-related genes, resulting in a susceptibility phenotype to Pst DC3000. Taken together, our results provide new insights for CAMTAs in peach fruit and indicate that PpCAMTA1 is associated with response to stresses during development.


Assuntos
Arabidopsis , Prunus persica , Arabidopsis/metabolismo , Calmodulina/metabolismo , Expressão Ectópica do Gene , Frutas/genética , Frutas/metabolismo , Regulação da Expressão Gênica de Plantas , Desenvolvimento Vegetal , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Prunus persica/genética , Prunus persica/metabolismo , Ácido Salicílico/metabolismo , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo
17.
ACS Appl Mater Interfaces ; 14(38): 43877-43885, 2022 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-36101984

RESUMO

The detection of molecules from highly diluted solutions with a limited amount is vital for precancer diagnosis, food safety, and forensic analysis. The sensitivity and convenience of detection techniques are the primary concerns. In this study, a hybrid superhydrophobic/-philic (SH/SHL) microporous platform is designed and fabricated by a femtosecond laser to improve surface-enhanced Raman scattering (SERS) performances. Relying on the micropores fabricated at the center of SHL patterns, sediments distributed at the central regions are avoided, leading to the further enrichment of the target molecules. The engineered micropores with high identification further improve the speed of Raman tests, and the fabricated SERS substrate shows an advantage in outdoor handheld detection and automated inspection applications. The optimized SERS sensor is sufficient for attomolar-level detection (10-17 M) of rhodamine 6G using analyte volumes of just 5 µL, corresponding to an enhancement factor of 5.19 × 1013. Meanwhile, a relative standard deviation of 7.48% at 10-10 M shows the excellent uniformity of this proposed SERS platform. This work further pushes forward the practical applications of SERS technology in ultratrace molecular detections.

18.
J Pharm Biomed Anal ; 220: 114984, 2022 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-35994945

RESUMO

A simple, rapid, sensitive and specific LC-MS/MS method was developed and validated for the quantitative determination of doxylamine in human plasma, using isotope doxylamine-d5 as internal standard (IS). The detection was conducted on a QTRAP 5500 tandem mass spectrometer coupled with electrospray ionization (ESI) source in positive ion mode. Quantification was achieved by positive electrospray ionization containing multiple reaction monitoring (MRM) transitions of m/z 271.0→182.0 for doxylamine and m/z 276.2→187.3 for IS. The mobile phase A was methanol, and mobile phase B was 20 mM ammonium acetate (0.2 % formic acid) in water, using a gradient elution procedure at a flow rate of 0.6 mL/min. The method was validated with a sensitivity of 0.500 ng/mL and a linear concentration range of 0.500-200 ng/mL. The inter-batch precision (%CV) was less than 5.4 %, and the accuracy deviation (%RE) ranged from - 10.6 % to 3.7 %; the inter-batch precision (%CV) was less than 6.6 %, and the accuracy deviation (%RE) was ranged from - 2.7 % to 0.1 %. The selectivity, sensitivity, extraction recovery, matrix effect, carryover, dilution reliability, stability and other characteristics were within the acceptable range. This validated method was successfully applied to a bioequivalence study that orally administered 25 mg of doxylamine succinate tablets in 60 healthy Chinese volunteers.


Assuntos
Doxilamina/sangue , Doxilamina/farmacocinética , Antagonistas dos Receptores Histamínicos H1/sangue , Antagonistas dos Receptores Histamínicos H1/farmacocinética , Espectrometria de Massas em Tandem/métodos , Administração Oral , China , Cromatografia Líquida/métodos , Doxilamina/administração & dosagem , Voluntários Saudáveis , Antagonistas dos Receptores Histamínicos H1/administração & dosagem , Humanos , Metanol , Reprodutibilidade dos Testes , Espectrometria de Massas por Ionização por Electrospray , Comprimidos , Equivalência Terapêutica
19.
Theor Appl Genet ; 135(10): 3455-3468, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35963933

RESUMO

KEY MESSAGE: The causal gene, CaHY5 of a chemical induced green-hypocotyl mutant was identified by molecular mapping. CaHY5 regulates anthocyanin accumulation by directly binding to the promoter of genes in anthocyanin pathway. Morphological markers at seedling stage are useful indicators for F1 hybrid seeds screening. Pepper is a worldwide vegetable with diverse uses, and F1 hybrids are popular in the pepper industry. Hypocotyl color is a useful marker to identify F1 hybrid seeds. However, most pepper accessions have purple hypocotyl caused by anthocyanin accumulation, while green hypocotyl pepper accessions are rare. In this study, we identified a green hypocotyl mutant (e1898) from a pepper ethylmethanesulfonate (EMS) mutant library. By combining bulked segregant RNA-seq (BSR), genome resequencing and recombinant analysis, it was found that CaHY5 is the causal gene of this mutant. Virus-induced gene silencing (VIGS) of CaHY5 resulted in the decrease of anthocyanin accumulation in pepper hypocotyls. RNA-seq data showed that many genes related to anthocyanin biosynthesis and transport decreased significantly in the mutant. Yeast one-hybrid (Y1H) assays showed that CaHY5 can bind to the promoter of CaF3H, CaF3'5'H, CaDFR, CaANS and CaGST, which are important genes in anthocyanin biosynthesis or transport. Our results indicate that CaHY5 directly regulates anthocyanin biosynthesis and transport, thus governing anthocyanin accumulation in pepper hypocotyl. The mutant and gene identified in this work shall be valuable in the purity control of hybrid pepper seeds.


Assuntos
Antocianinas , Capsicum , Capsicum/genética , Regulação da Expressão Gênica de Plantas , Hipocótilo/genética , Hipocótilo/metabolismo , Mutação , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo
20.
Anim Nutr ; 11: 15-24, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36016965

RESUMO

To study the effects of dietary energy level on the meat quality of different muscles in finishing pigs, 400 Xiangcun Black pigs (BW = 79.55 ± 4.77 kg) were randomly assigned to 5 treatments with varied calculated digestive energy (DE) at 3,050, 3,100, 3,150, 3,200 and 3,250 kcal/kg, respectively. Each treatment had 8 replicates with 10 pigs per replicate. Meat quality, amino acid and fatty acid composition were tested in this study. No differences in average daily gain, average daily feed intake or feed-to-gain ratio (P > 0.05) were observed among dietary treatments. Glycogen concentrations of longissimus dorsi (LD) muscle in DE3150 was higher than those in other groups (P < 0.05). The crude fat concentration of biceps femoris (BF) muscle in DE3250 tended to be higher than that in DE3150 and DE3100 groups (P < 0.05). Pigs in DE3250 and DE3200 had higher fiber density and smaller cross-sectional area of BF muscle than those in DE3150 (P < 0.05). Pigs in DE3150 had the highest Cu concentration in LD muscle compared with those in DE3200, DE3250 (P < 0.05). The C16:1 proportion of LD muscle was lower (P < 0.01) and C20:1 was higher (P < 0.05) in DE3050 than that in the other dietary treatments. The C18:3n6 and C20:3n6 proportions of BF muscle in DE3150 were higher than those in DE 3050, DE3200 and DE3250 (P < 0.05). For LD muscle, mRNA expressions of type I and IIa MyHC in group DE3150 were higher than other treatments (P < 0.01). The LD muscle in DE3150 expressed higher PPARd than in other groups (P < 0.01). Pigs in DE3100 expressed higher FOX1 than in DE3200 and DE3250 (P < 0.05). Sterol-regulatory element binding proteins (SREBPa) mRNA expression decreased linearly when dietary energy level increased in BF muscle (P < 0.01). In conclusion, a 200 kcal/kg decrease in digestible energy for 4 consecutive weeks did not affect growth performance of Xiangcun Black pigs. Furthermore, LD and BF muscle respond differently to dietary energy level, and meat quality was improved by the medium energy level during the finishing phase.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...