Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 36
Filtrar
1.
Curr Med Res Opin ; 37(6): 917-927, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-33729889

RESUMO

BACKGROUND: To develop a sensitive and clinically applicable risk assessment tool identifying coronavirus disease 2019 (COVID-19) patients with a high risk of mortality at hospital admission. This model would assist frontline clinicians in optimizing medical treatment with limited resources. METHODS: 6415 patients from seven hospitals in Wuhan city were assigned to the training and testing cohorts. A total of 6351 patients from another three hospitals in Wuhan, 2169 patients from outside of Wuhan, and 553 patients from Milan, Italy were assigned to three independent validation cohorts. A total of 64 candidate clinical variables at hospital admission were analyzed by random forest and least absolute shrinkage and selection operator (LASSO) analyses. RESULTS: Eight factors, namely, Oxygen saturation, blood Urea nitrogen, Respiratory rate, admission before the date the national Maximum number of daily new cases was reached, Age, Procalcitonin, C-reactive protein (CRP), and absolute Neutrophil counts, were identified as having significant associations with mortality in COVID-19 patients. A composite score based on these eight risk factors, termed the OURMAPCN-score, predicted the risk of mortality among the COVID-19 patients, with a C-statistic of 0.92 (95% confidence interval [CI] 0.90-0.93). The hazard ratio for all-cause mortality between patients with OURMAPCN-score >11 compared with those with scores ≤ 11 was 18.18 (95% CI 13.93-23.71; p < .0001). The predictive performance, specificity, and sensitivity of the score were validated in three independent cohorts. CONCLUSIONS: The OURMAPCN score is a risk assessment tool to determine the mortality rate in COVID-19 patients based on a limited number of baseline parameters. This tool can assist physicians in optimizing the clinical management of COVID-19 patients with limited hospital resources.


Assuntos
COVID-19 , Medição de Risco/métodos , COVID-19/epidemiologia , COVID-19/mortalidade , China , Hospitalização/estatística & dados numéricos , Humanos , Itália , Fatores de Risco
2.
Med (N Y) ; 2(1): 38-48.e2, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33043313

RESUMO

Background: The coronavirus disease 2019 (COVID-19) is a recently emerged respiratory infectious disease with kidney injury as a part of the clinical complications. However, the dynamic change of kidney function and its association with COVID-19 prognosis are largely unknown. Methods: In this multicenter retrospective cohort study, we analyzed clinical characteristics, medical history, laboratory tests, and treatment data of 12,413 COVID-19 patients. The patient cohort was stratified according to the severity of the outcome into three groups: non-severe, severe, and death. Findings: The prevalence of elevated blood urea nitrogen (BUN), elevated serum creatinine (Scr), and decreased blood uric acid (BUA) at admission was 6.29%, 5.22%, and 11.66%, respectively. The trajectories showed the elevation in BUN and Scr levels, as well as a reduction in BUA level for 28 days after admission in death cases. Increased all-cause mortality risk was associated with elevated baseline levels of BUN and Scr and decreased levels of BUA. Conclusions: The dynamic changes of the three kidney function markers were associated with different severity and poor prognosis of COVID-19 patients. BUN showed a close association with and high potential for predicting adverse outcomes in COVID-19 patients for severity stratification and triage. Funding: This study was supported by grants from the National Key R&D Program of China (2016YFF0101504), the National Science Foundation of China (81630011, 81970364, 81970070, 81970011, 81870171, and 81700356), the Major Research Plan of the National Natural Science Foundation of China (91639304), the Hubei Science and Technology Support Project (2019BFC582, 2018BEC473, and 2017BEC001), and the Medical Flight Plan of Wuhan University.

4.
Hepatology ; 72(2): 389-398, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32359177

RESUMO

BACKGROUND AND AIMS: Coronavirus disease 2019 (COVID-19) is a new infectious disease. To reveal the hepatic injury related to this disease and its clinical significance, we conducted a multicenter retrospective cohort study that included 5,771 adult patients with COVID-19 pneumonia in Hubei Province. APPROACH AND RESULTS: We reported the distributional and temporal patterns of liver injury indicators in these patients and determined their associated factors and death risk. Longitudinal liver function tests were retrospectively analyzed and correlated with the risk factors and death. Liver injury dynamic patterns differed in alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total bilirubin (TBIL). AST elevated first, followed by ALT, in severe patients. ALP modestly increased during hospitalization and largely remained in the normal range. The fluctuation in TBIL levels was mild in the non-severe and the severe groups. AST abnormality was associated with the highest mortality risk compared with the other indicators of liver injury during hospitalization. Common factors associated with elevated liver injury indicators were lymphocyte count decrease, neutrophil count increase, and male gender. CONCLUSION: The dynamic patterns of liver injury indicators and their potential risk factors may provide an important explanation for the COVID-19-associated liver injury. Because elevated liver injury indicators, particularly AST, are strongly associated with the mortality risk, our study indicates that these parameters should be monitored during hospitalization.


Assuntos
Betacoronavirus , Infecções por Coronavirus/mortalidade , Infecções por Coronavirus/fisiopatologia , Fígado/fisiopatologia , Pneumonia Viral/mortalidade , Pneumonia Viral/fisiopatologia , Adulto , Idoso , Alanina Transaminase/sangue , Fosfatase Alcalina/sangue , Aspartato Aminotransferases/sangue , Bilirrubina/sangue , Biomarcadores , COVID-19 , Feminino , Humanos , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Pandemias , Estudos Retrospectivos , SARS-CoV-2
5.
J Mol Recognit ; 33(3): e2816, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31945234

RESUMO

Cefixime is a third generation orally administered cephalosporin that is frequently used as a broad spectrum antibiotic against various gram-negative and gram-positive bacteria. In this study, a simple and sensitive fluorescent sensor for the determination of the cefixime and ctDNA was established based on the CdTe:Zn2+ quantum dots (QDs). The fluorescence of CdTe:Zn2+ QDs can be effectively quenched by cefixime in virtue of the surface binding of cefixime on CdTe:Zn2+ QDs and the subsequent photoinduced electron transfer process from CdTe:Zn2+ QDs to cefixime, in particular, the high sensitivity of QDs fluorescence emission to cefixime at the micromole per liter level, which render the cefixime-CdTe:Zn2+ QDs system into fluorescence "OFF" status, then turn on in the presence of ctDNA. Furthermore, the Fourier transform infrared (FTIR) spectra of characteristic bands of C-N and N-H groups of cefixime endow evidence for the interaction of cefixime with CdTe:Zn2+ QDs. The relative electrochemical behavior of the affinity of CdTe:Zn2+ QDs for cefixime and ctDNA reveals the potential molecular binding mechanism.


Assuntos
Técnicas Biossensoriais , Compostos de Cádmio/química , Cefixima/isolamento & purificação , DNA Tumoral Circulante/isolamento & purificação , Telúrio/química , Cefixima/sangue , Cefixima/química , DNA Tumoral Circulante/sangue , DNA Tumoral Circulante/química , Transporte de Elétrons/efeitos dos fármacos , Fluorescência , Humanos , Pontos Quânticos/química , Espectrometria de Fluorescência , Espectroscopia de Infravermelho com Transformada de Fourier
6.
Luminescence ; 33(1): 209-218, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-28976065

RESUMO

Cephalosporins belong the largest class of antibiotics used in the treatment of a wide range of infectious diseases caused by susceptible organisms. In the present study, we chose two typical antibiotics cefalexin/cefixime based on their structure, and investigated the interaction of cephalexin/cefixime with bovine serum albumin (BSA) using UV-vis absorption spectra, fluorescence spectroscopy, circular dichroism (CD) spectroscopy and molecular modeling approaches. Spectroscopic experiments revealed the formation of a BSA - cefalexin/cefixime complex. The binding parameters calculated using a modified Stern - Volmer method and the Scatchard method reached 103 -104  L·mol-1 . Thermodynamic parameter studies revealed that binding characteristics by negative enthalpy and positive entropy changes, and electrostatic interactions play a major role. Site marker competitive displacement experiments and molecular modeling approaches demonstrated that cefalexin and cefixime bind with appropriate affinity to site I (subdomain IIA) of BSA. Furthermore, synchronous fluorescence spectra, CD spectra and molecular modeling results indicated that the secondary structure of BSA was changed in the presence of cefalexin and cefixime. Additionally, the effects of metal ions on the BSA - cefalexin/cefixime system were also assessed.


Assuntos
Cefixima/química , Cefalexina/química , Soroalbumina Bovina/química , Animais , Bovinos , Dicroísmo Circular , Modelos Moleculares , Conformação Molecular , Estrutura Molecular , Espectrometria de Fluorescência , Espectrofotometria Ultravioleta , Termodinâmica
7.
J Mol Recognit ; 31(5): e2691, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-29210128

RESUMO

Tremendous research efforts have been dedicated to fabricating high-quality Zn-doped CdTe quantum dots (QDs) for any potential biomedical applications. In particular, the correlation of issues regarding how QDs interact with DNA is of greatest importance. Herein, a pH-responsive study of the interactions between CdTe:Zn2+ quantum dots with 4 different sizes and calf thymus DNA (ctDNA) was conducted using multispectroscopic techniques and electrochemical investigation. Fluorescence studies revealed that this interaction process is predominantly a static process and groove binding was the main binding mode for CdTe:Zn2+ QDs to ctDNA. The calculated negative values of enthalpy (-45.06 kJ mol-1 ) and entropy (-133.62 J mol-1  K-1 ) with temperature changes indicated that the hydrogen bonds and van der Waals interactions played major roles in the reaction. Furthermore, circular dichroism spectroscopy and Fourier transform infrared spectrometry analyses indicate that the normal conformation of ctDNA is discombobulated by CdTe:Zn2+ QDs. In addition, the electrochemical behavior of the affinity of CdTe:Zn2+ QDs for ctDNA agreed well with the results obtained from fluorescence experiments. This study might be meaningful for understanding the molecular binding mechanism of QDs for DNA and provides a basis for QD-labeled systems.


Assuntos
Compostos de Cádmio/síntese química , DNA/química , Telúrio/química , Zinco/química , Compostos de Cádmio/química , Técnicas Eletroquímicas , Ligação de Hidrogênio , Tamanho da Partícula , Pontos Quânticos , Espectrometria de Fluorescência , Espectrofotometria Ultravioleta
8.
J Sep Sci ; 37(23): 3555-63, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25216109

RESUMO

With a given free-flow electrophoresis device, reasonable conditions (electric field strength, carrier buffer conductivity, and flow rate) are crucial for an optimized separation. However, there has been no experimental study on how to choose reasonable general conditions for a free-flow electrophoresis device with a thermoelectric cooler in view of Joule heat generation. Herein, comparative experiments were carried out to propose the selection procedure of general conditions in this study. The experimental results demonstrated that appropriate conditions were (i) <67 V/cm electric field strength; (ii) lower than 1.3 mS/cm carrier buffer conductivity (Tris-HCl: 20 mM Tris was titrated by HCl to pH 8.0); and (iii) higher than 3.6 mL/min carrier buffer flow rate. Furthermore, under inappropriate conditions (e.g. 400 V voltage and 40 mM Tris-HCl carrier buffer), the free-flow electrophoresis separation would be destroyed by bubbles caused by more Joule heating. Additionally, a series of applications under the appropriate conditions were performed with samples of model dyes, proteins (bovine serum albumin, myoglobin, and cytochrome c), and cells (Escherichia coli, Streptococcus thermophilus, and Saccharomyces cerevisiae). The separation results showed that under the appropriate conditions, separation efficiency was obviously better than that in the previous experiments with randomly or empirically selected conditions.


Assuntos
Eletroforese/instrumentação , Animais , Bactérias/química , Bovinos , Citocromos c/química , Citocromos c/isolamento & purificação , Eletroforese/métodos , Humanos , Mioglobina/química , Mioglobina/isolamento & purificação , Soroalbumina Bovina/química , Soroalbumina Bovina/isolamento & purificação
9.
J Sep Sci ; 37(11): 1359-63, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24648284

RESUMO

Uneven flow in free-flow electrophoresis (FFE) with a gravity-induced fraction collector caused by air bubbles in outlets and/or imbalance of the surface tension of collecting tubes would result in a poor separation. To solve these issues, this work describes a novel collector for FFE. The collector is composed of a self-balance unit, multisoft pipe flow controller, fraction collector, and vacuum pump. A negative pressure induced continuous air flow rapidly flowed through the self-balance unit, taking the background electrolyte and samples into the fraction collector. The developed collector has the following advantages: (i) supplying a stable and harmonious hydrodynamic environment in the separation chamber for FFE separation, (ii) effectively preventing background electrolyte and sample flow-back at the outlet of the chamber and improving the resolution, (iii) increasing the preparative scale of the separation, and (iv) simplifying the operation. In addition, the cost of the FFE device was reduced without using a multichannel peristaltic pump for sample collection. Finally, comparative FFE experiments on dyes, proteins, and cells were carried out. It is evident that the new developed collector could overcome the problems inherent in the previous gravity-induced self-balance collector.


Assuntos
Eletroforese/instrumentação , Corantes/análise , Eletroforese/métodos , Hidrodinâmica , Pressão , Proteínas/análise
10.
J Chromatogr A ; 1321: 119-26, 2013 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-24246174

RESUMO

Complex assembly, inconvenient operations, poor control of Joule heating and leakage of solution are still fundamental issues greatly hindering application of free-flow electrophoresis (FFE) for preparative purpose in bio-separation. To address these issues, a novel FFE device was developed based on our previous work. Firstly, a new mechanical structure was designed for compact assembly of separation chamber, fast removal of air bubble, and good anti-leakage performance. Secondly, a highly efficient thermoelectric cooling system was used for dispersing Joule heating for the first time. The systemic experiments revealed the three merits: (i) 3min assembly without any liquid leakage, 80 times faster than pervious FFE device designed by us or commercial device (4h); (ii) 5s removing of air bubble in chamber, 1000-fold faster than a normal one (2h or more) and (iii) good control of Joule heating by the cooling system. These merits endowed the device high stable thermo- and hydro-dynamic flow for long-term separation even under high electric field of 63V/cm. Finally, the developed device was used for up to 8h continuous separation of 5mg/mL fuchsin acid and purification of three model proteins of phycocyanin, myoglobin and cytochrome C, demonstrating the applicability of FFE. The developed FFE device has evident significance to the studies on stem cell, cell or organelle proteomics, and protein complex as well as micro- or nano-particles.


Assuntos
Temperatura Baixa , Eletroforese em Gel de Poliacrilamida/instrumentação , Desenho de Equipamento
11.
Artigo em Chinês | MEDLINE | ID: mdl-22675937

RESUMO

OBJECTIVE: To study the differentiation of Th1/Th2 on the levels of transcription factors and cytokines production in patients with laryngeal squamous cell carcinoma in peripheral blood mononuclear cells (PBMC). In addition, the relation of p53 and T-bet, GATA3 expression was discussed for understanding the role of T-bet and GATA3 in metastasis. METHOD: The gene expression of Th1/Th2 type transcription factors T-bet, GATA3 and cytokines IFN-gamma, IL-4 were determined by RT-PCR and realtime RT-PCR from 49 patients with laryngeal squamous cell carcinoma and 30 normal controls. The expression of p53 was analyzed by immunohistochemistry. RESULT: Predominant expression of Th2 type cytokines was detected in 49 laryngeal squamous cell carcinoma patients. The expression rates of T-bet, GATA3, IFN-gamma and IL-4 was 42.86% (21/49), 71.43% (35/49), 26.53% (13/49), 63.27% (33/49) respectively. The expression rates of T-bet and IFN-gamma in patients were lower than in control group (P < 0.05), but the results of GATA3 and IL-4 were opposite (P < 0.05). The similar results were obtained by realtime PCR. The expression of p53 in patients was accompanied with lower expression of T-betmRNA and higher expression of GATA3mRNA. CONCLUSION: There is predominant expression of Th2 type transcription factors and cytokines in PBMC of laryngeal squamous cell carcinoma patients. T-bet and GATA3 can be used as reference indicators for the metastasis of laryngeal squamous cell carcinoma.


Assuntos
Carcinoma de Células Escamosas/metabolismo , Neoplasias Laríngeas/metabolismo , Células Th1/metabolismo , Células Th2/metabolismo , Adulto , Idoso , Carcinoma de Células Escamosas/patologia , Estudos de Casos e Controles , Feminino , Fator de Transcrição GATA3/metabolismo , Humanos , Interferon gama/metabolismo , Interleucina-4/metabolismo , Neoplasias Laríngeas/patologia , Leucócitos Mononucleares/metabolismo , Masculino , Pessoa de Meia-Idade , RNA Mensageiro/genética , Proteínas com Domínio T/metabolismo , Proteína Supressora de Tumor p53/metabolismo
12.
Artigo em Chinês | MEDLINE | ID: mdl-22010335

RESUMO

OBJECTIVE: To investigate the utilization of functional surgery in benign parotid tumor of superficial lobe. METHOD: Conventional parotidectomy were modified in the following aspects: incision, preservation of great auricular nerve, local facial nerve dissection, partial superficial parotidectomy and repairing of sunken deformity. The post-operative recurrence incidence, functional outcomes and complications of 21 patients with benign parotid tumor of superficial lobe undergoing functional surgery were analyzed retrospectively. RESULT: No recurrence of tumor was found in follow-ups. No obvious scar and sunken deformity was found. The area with abnormal sensory around the auricle was smaller compared with those whose great auricular nerve had not been preserved. Post-operative transient facial paralysis was observed in 4 cases and all recovered in 3 months. One case complained about Frey's syndrome. Salivary leakage was found on 1 case. CONCLUSION: Compared with conventional surgery, functional surgery for benign parotid tumor of superficial lobe may improve cosmetic, sensory, salivary function, facial nerve function and quality of life without raising post-operative recurrence.


Assuntos
Glândula Parótida/cirurgia , Neoplasias Parotídeas/cirurgia , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias/epidemiologia , Estudos Retrospectivos , Adulto Jovem
13.
Artigo em Chinês | MEDLINE | ID: mdl-20518286

RESUMO

OBJECTIVE: To evaluate the utilization of functional neck dissection for treatment of recurrent branchial cleft anomalies. METHOD: Fifteen patients with recurrent branchial cleft anomalies (fistula and cyst) undergoing functional neck dissection were retrospectively analyzed. RESULT: Complications included 2 incisions secondary healing, 1 postoperative choking persisting for 1 months and 1 Horner's syndrome. There was no recurrence after a follow up from 2 months to 6 years,except 2 cases were lost to follow-up. CONCLUSION: Functional neck dissection is an effective and safe surgical management for recurrent second and third branchial cleft anomalies(fistula and cyst).


Assuntos
Região Branquial/anormalidades , Região Branquial/cirurgia , Esvaziamento Cervical/métodos , Adolescente , Adulto , Criança , Feminino , Humanos , Masculino , Pescoço/cirurgia , Recidiva , Estudos Retrospectivos , Adulto Jovem
14.
Artigo em Chinês | MEDLINE | ID: mdl-20120865

RESUMO

OBJECTIVE: In order to prevent misdiagnosis and degrade death rate, we explored the early diagnosis and correct treatment methods of traumatic pseudoaneurysm of internal carotid artery in sphenoid sinus. METHOD: The clinic data of 6 cases who had traumatic pseudoaneurysm of internal carotid artery in sphenoid sinus and were admitted in our hospital were analyzed retrospectively. Large numbers of literature about the disease were reviewed. Some early diagnosis methods and optimal treatment schemes were proposed. RESULT: All patients had a history of cranium trauma and recurrent attacks nasal hemorrhage. There are two patients with a complication of sight loss in single eye. In the six cases, one case was treated with unilateral common carotid artery ligation, three cases were treated with aneurysm and internal carotid artery embolism by using sacculus proprius which is able to shedding, one case was treated with internal carotid artery embolism by using tiny circlip ring. These five patients were cured with no severe complication. One patient death of hemorrhage. His nasal cavity was tamponade repeatedly because repeat nasal hemorrhage. However, he had never treated with internal carotid artery embolism. CONCLUSION: The patients who have traumatic pseudoaneurysm of internal carotid artery usually die of unmanageable hemorrhage. The patients may be cured if they could obtain an early diagnosis and correct treatment.


Assuntos
Artéria Carótida Interna , Fístula Carotidocavernosa/diagnóstico , Fístula Carotidocavernosa/terapia , Adulto , Fístula Carotidocavernosa/etiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Seio Esfenoidal , Adulto Jovem
15.
Artigo em Chinês | MEDLINE | ID: mdl-18800677

RESUMO

OBJECTIVE: To investigate the expression of COX-2 in both molecule and protein levels in laryngeal squamous cell carcinoma and the relationship between its intensity and histological grade, clinical stage and lymphoma metastasis. METHOD: By using emi-quantify RT-PCR and Immunohistochemistry, the expression of the COX-2 mRNA and COX-2 protein in laryngeal squamous cell carcinoma and polyp of vocal cord were examined. RESULT: Compared with the polyp of vocal cord tissue, the expression level of COX-2 mRNA and COX-2 protein in human laryngeal squamous cell carcinoma were significantly increased (P < 0.01). The intensity of COX-2 mRNA and protein expression increased as the laryngeal squamous cell carcinoma progresses developed. CONCLUSION: COX-2 is high expressed in human laryngeal squamous cell carcinoma, and it may play an significant role in the growth, invasion and metastasis of human laryngeal squamous cell carcinoma. A combination of emi-quantify RT-PCR and immunohistochemistry is sensitive and specific for laryngeal neoplasms detection.


Assuntos
Carcinoma de Células Escamosas/metabolismo , Ciclo-Oxigenase 2/metabolismo , Neoplasias Laríngeas/metabolismo , Adulto , Idoso , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/patologia , Ciclo-Oxigenase 2/genética , Feminino , Humanos , Neoplasias Laríngeas/genética , Neoplasias Laríngeas/patologia , Masculino , Pessoa de Meia-Idade , RNA Mensageiro/genética
16.
Artigo em Chinês | MEDLINE | ID: mdl-18727519

RESUMO

OBJECTIVE: To study the expressions and clinical significance of integrin beta1 and integrin-linked kinase(ILK) in laryngeal carcinoma (LSCC). METHOD: The mRNA and protein levels of integrinbeta1 and integrin-linked kinase in 43 specimens of laryngeal carcinoma, its surrounding tissue and 18 specimens of vocal cord polyp were detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry SABC method. RESULT: The mRNA levels of integrin beta1 and ILK protein were significantly higher in laryngeal carcinoma than that in its surrounding tissue and vocal cord polyp (P < 0.05). There was significant correlation between the levels of integrin beta1 expression and the status of cervical lymph node, either between the level of ILK expression and cervical lymph node status (P < 0.05). There was significant correlation between the levels of integrin beta1 expression and T-category, either between the level of ILK expression and T-category (P < 0.05). But pathological grades was not significantly related with the level of integrin beta1 or ILK expression (P > 0.05). CONCLUSION: The expressions of integrin beta1 and ILK are increased in LSCC. Integrin beta1 and ILK may contribute significantly to invasion and metastasis of LSCC.


Assuntos
Carcinoma de Células Escamosas/metabolismo , Integrina beta1/metabolismo , Neoplasias Laríngeas/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo , Adulto , Idoso , Carcinoma de Células Escamosas/patologia , Feminino , Humanos , Neoplasias Laríngeas/patologia , Linfonodos/patologia , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias
17.
Artigo em Chinês | MEDLINE | ID: mdl-19297850

RESUMO

OBJECTIVE: To study the expressions and clinical significance of integrin beta1 and focal adhesion kinase (FAK) in laryngeal carcinoma (LSCC). METHOD: The mRNA and protein levels of integrin beta1 and focal adhesion kinase and the surrounding tissue of laryngeal carcinoma in 48 specimens and 20 specimens of vocal cord polyp were detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry SABC method. RESULT: The mRNA levels and positive rates of integrin beta1 and FAK protein were significantly higher in laryngeal carcinoma than that in the surrounding tissue and vocal cord polyp (P < 0.05). The expression levels of integrin beta1 and FAK were significantly higher in the group with cervical lymph node metastasis than those without (P < 0.05), and they were significantly higher in the tissue of stage of T3 and T4 than those of T1 and T2 (P < 0.05). But pathological grades was not significantly related with the expression levels of integrin beta1 or FAK (P > 0.05). CONCLUSION: The expression levels of integrin beta1 and FAK were increased in LSCC, and they may contribute significantly to invasion and metastasis of LSCC.


Assuntos
Carcinoma de Células Escamosas/patologia , Quinase 1 de Adesão Focal/metabolismo , Integrina beta1/metabolismo , Neoplasias Laríngeas/patologia , Adulto , Idoso , Carcinoma de Células Escamosas/metabolismo , Feminino , Humanos , Neoplasias Laríngeas/metabolismo , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias
18.
Artigo em Chinês | MEDLINE | ID: mdl-17580716

RESUMO

OBJECTIVE: To investigate the optimal surgical approach for resecting infratemporal fossa-pterygomaxillary fossa-parapharyngeal space tumor. The aim of this study is to enhance therapeutic effect and reduce complications and sequelae. METHOD: Sixty-six patients with infratemporal fossa-pterygomaxillary fossa-parapharyngeal space tumor were analyzed retrospectively from 1998-2004, including complains, symptoms, physical signs; histodiagnosis, image examinations and surgical approaches. RESULT: No recurrence was found in fifty benign tumor cases after 2 to 5 years, while in 16 cases with malignant tumor, four patients died in one year, 8 cases died in 2 to 4 years, and only 4 patients' survival time exceeded 4 years. CONCLUSION: Extended maxillectomy is suitable for resecting primary carcinoma of maxillary sinus invading pterygomaxillary fossa, infratemporal fossa and /or parapharyngeal space tumor. also midface degloving approach is suitable for nasal primary cavity, nasal sinuses, nasopharynx and/or pterygomaxillary fossa tumor and localized malignant tumor. Trans-cervical combining mandibular split swing approach is suitable for parapharyngeal space tumor invading pterygomaxillary fossa and/or infratemporal fossa tumor. Trans-cervical jaw combining mandibulotomy is suitable for resecting parapharyngeal space, infratemporal fossa tumor for invading lateral skull base and pterygomaxillary fossa tumor.


Assuntos
Craniotomia/métodos , Neoplasias da Base do Crânio/cirurgia , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Procedimentos Cirúrgicos Otorrinolaringológicos/métodos , Estudos Retrospectivos
19.
Lin Chuang Er Bi Yan Hou Ke Za Zhi ; 20(18): 820-1, 2006 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-17144487

RESUMO

OBJECTIVE: The aim of this study was to discuss the diagnosis and treatment of special airway foreign body. METHOD: Clinical data of 69 patients with airway foreign body were analyzed,of which 45 patients underwent rigid bronchoscopy while another 24 patients underwent tracheotomy. RESULT: The operations succeeded in 43 of the 45 patients who underwent rigid bronchoscopy, while the other 2 patients died of respiratory failure because of the foreign body slipping off at the level of the glottis. All of the 24 other patients were cured, in which continuous saturation of blood oxygen below 90% occurred in 8 patients and turned normal after positive airway pressure in intensive care unit (ICU). The total cure rate reached 97.1%. CONCLUSION: Most of special foreign body in the airway can be taken out through tracheotomy under general anesthesia. If the foreign body is too big and irregularly, or the patients is too young or have the pulmonary atelectasis, it is much safer to remove foreign body by tracheotomy.


Assuntos
Corpos Estranhos/diagnóstico , Corpos Estranhos/cirurgia , Sistema Respiratório , Adolescente , Adulto , Idoso , Broncoscopia , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Traqueotomia , Adulto Jovem
20.
Lin Chuang Er Bi Yan Hou Ke Za Zhi ; 20(19): 885-7, 2006 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-17168116

RESUMO

OBJECTIVE: To investigate the diagnostic value of CT and MRI in tumors of parapharyngeal space (PPS). METHOD: The data of one hundred and thirty-eight patients with PPS tumor treated in our department during the period from January, 1988 to January, 2005 were collected and reviewed. Eighty-three men and 55 women were enrolled, not including patients with metastasis tumors in PPS or nasopharyngeal carcinomas invading PPS. The age varies from 7 to 73 yrs(average 44.7 yrs). Among the patients, 12 were operated for the second time because of recurrence. All the patients were examined by CT or/and MRI. RESULT: CT and MRI could provide the position, size, confine of the tumor and its relationship with cervical blood vessels well, which were important to operation schemes. CONCLUSION: CT and MRI before operation are valuable to the treatment of PPS tumors.


Assuntos
Neoplasias Nasofaríngeas/diagnóstico , Adolescente , Adulto , Idoso , Criança , Fáscia , Feminino , Humanos , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Neoplasias Nasofaríngeas/diagnóstico por imagem , Faringe , Tomografia Computadorizada por Raios X , Adulto Jovem
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...