Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 2.582
Filtrar
1.
Diabetes Metab Res Rev ; : e3496, 2021 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-34537998

RESUMO

AIMS: We prospectively evaluated the association of circulating retinol-binding protein 4 (RBP4) levels in early pregnancy and risk of incident gestational diabetes mellitus (GDM) in pregnant women. METHODS: A nested case-control study was conducted among 332 women who developed GDM and 664 matched controls based on the Tongji-Shuangliu Birth Cohort. GDM was diagnosed during 24-28 weeks of gestation according to the International Association of Diabetes and Pregnancy Study Group criteria. Serum RBP4 levels in early pregnancy (6-15 weeks of gestation) were determined by ELISA assay. Multivariable conditional logistic regression models were used to analyze the association and generated the odds ratio (OR) and 95% confidence interval (CI). EMBASE and PubMed were searched up to November 30, 2020 to identify studies investigating the association between blood RBP4 levels in early pregnancy and incident GDM. RESULTS: In the multivariable model with adjustment of potential risk factors, the OR comparing the extreme quartiles of serum RBP4 levels was 2.26 (95% CI: 1.34, 3.81; P for trend <0.001), and each SD (SD) increment of RBP4 was associated with 1.39-fold (95% CI: 1.15, 1.69) higher risk of GDM. The results were confirmed in a meta-analysis that included additional four studies with an overall OR of 1.47 (95% CI: 1.18, 1.83) per 1-SD increment of RBP4. CONCLUSIONS: Serum RBP4 levels in early pregnancy, independent of metabolic risk factors, are positively associated with the risk of GDM in pregnant women. Our findings may provide new insights into the mechanisms underlying the etiology of GDM. This article is protected by copyright. All rights reserved.

2.
EMBO Mol Med ; : e11668, 2021 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-34542937

RESUMO

Loss of ß-cell number and function is a hallmark of diabetes. ß-cell preservation is emerging as a promising strategy to treat and reverse diabetes. Here, we first found that Pdia4 was primarily expressed in ß-cells. This expression was up-regulated in ß-cells and blood of mice in response to excess nutrients. Ablation of Pdia4 alleviated diabetes as shown by reduced islet destruction, blood glucose and HbA1c, reactive oxygen species (ROS), and increased insulin secretion in diabetic mice. Strikingly, this ablation alone or in combination with food reduction could fully reverse diabetes. Conversely, overexpression of Pdia4 had the opposite pathophysiological outcomes in the mice. In addition, Pdia4 positively regulated ß-cell death, dysfunction, and ROS production. Mechanistic studies demonstrated that Pdia4 increased ROS content in ß-cells via its action on the pathway of Ndufs3 and p22phox . Finally, we found that 2-ß-D-glucopyranosyloxy1-hydroxytrideca 5,7,9,11-tetrayne (GHTT), a Pdia4 inhibitor, suppressed diabetic development in diabetic mice. These findings characterize Pdia4 as a crucial regulator of ß-cell pathogenesis and diabetes, suggesting Pdia4 is a novel therapeutic and diagnostic target of diabetes.

3.
Transl Psychiatry ; 11(1): 454, 2021 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-34480014

RESUMO

Ketamine has been demonstrated to be a rapid-onset and long-lasting antidepressant, but its underlying molecular mechanisms remain unclear. Recent studies have emerged microRNAs as important modulators for depression treatment. In this study, we report that miR-98-5p is downregulated in the prefrontal cortex and hippocampus of mice subjected to chronic social stress, while overexpressing it by its agonist alleviates depression-like behaviors. More importantly, we demonstrate that miR-98-5p is upregulated by ketamine administration, while inhibition of it by its antagonist blocks the antidepressant effect of ketamine. Our data implicate a novel molecular mechanism underlying the antidepressant effect of ketamine, and that therapeutic strategies targeting miR-98-5p could exert beneficial effects for depression treatment.

4.
Zhongguo Zhong Yao Za Zhi ; 46(12): 3144-3149, 2021 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-34467706

RESUMO

The effect of intestinal flora changes on the pharmacokinetics of astragaloside Ⅳ in rats with type 2 diabetes mellitus was explored in this study. The rat model in preliminary experiment was established by high-sugar and high-fat diet combined with the intraperitoneal injection of low-dose streptozotocin(STZ). Rats were divided into model group, astragaloside Ⅳ group, berberine group and combination group(five rats in each group). After two weeks of gavage, the rats' feces was taken for 16 S rRNA sequencing of intestinal flora. Pharmacokinetic experiments were performed on astragaloside Ⅳ in the four groups one day after the preliminary experiment. Plasma samples were precipitated in methanol with ginsenoside Rb_1 as an internal standard, and the plasma concentrations of astragaloside Ⅳ at different time points were determined by UPLC-MS/MS. The chromatographic separation was performed on a Waters Acquity UPLC BEH-C_(18) column(2.1 mm×100 mm, 1.7 µm) via gradient elution. The mobile phase was acetonitrile(A) and 5 mmol·L~(-1) ammonium formate solution with 0.2% formic acid(B). The flow rate was 0.4 mL·min~(-1), the injection volume 5 µL and the column temperature 40 ℃. The mass spectrometry was carried out with electrospray ionization source(ESI) in multiple reaction monitoring and positive ion modes. The specificity, linearity range, accuracy, precision, stability and dilution effect of the method all met the requirements for the determination of astragaloside Ⅳ in plasma. Plasma concentration-time curves were plotted and relevant pharmacokinetic parameters were calculated by DAS 3.2.8. The results showed that the concentration of absorbed astragaloside Ⅳ increased within 0-3.95 h and began to decline since 3.95 h. After 36 h, the metabolism was complete. The area under the plasma concentration-time curve(AUC_(0-t)) and the peak concentration(C_(max)) of astragaloside Ⅳ were increased in the three administration groups compared with the model group, but without significant difference, which suggested that the pharmacokinetic characteristics of saponin components would not necessarily change after the drug-induced alteration of intestinal flora.


Assuntos
Diabetes Mellitus Tipo 2 , Microbioma Gastrointestinal , Saponinas , Animais , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Ratos , Ratos Sprague-Dawley , Reprodutibilidade dos Testes , Espectrometria de Massas em Tandem , Triterpenos
5.
Nanoscale ; 2021 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-34516594

RESUMO

Extensive studies on lead halide perovskites have shown that these materials are excellent candidates as gain mediums. Recently, many efforts have been made to incorporate perovskite lasers in integrated optical circuits. Possible solutions would be to utilize standard lithography with an etching/lift-off process or a direct laser etching technique. However, due to the fragile nature of the lead halide perovskites which gives rise to significant material deterioration during the lithography and etching processes, realizing a small-size, low-roughness, and single-mode laser remains a challenge. Here, a lithographic in-mold patterning method realized by nanocrystal concentration control and a multi-step filling-drying process is proposed to demonstrate CsPbBr3 nanocrystals distributed-Bragg-reflector (DBR) waveguide lasers. This method realizes the patterning of the CsPbBr3 nanocrystal laser cavity and DBR grating without lift-off and etching processes, and the smallest fabricated structures are obtained in a few hundred nanometers. The single-mode lasing is demonstrated at room temperature with a threshold of 23.5 µJ cm-2. The smallest full width at half maximum FWHM of the laser output is 0.4 nm. Due to the fabrication process and the DBR laser geometry, the lasers can be fabricated in a compact array, which is important for incorporating perovskite-based lasers in complex optoelectronic circuits.

6.
Med Sci Monit ; 27: e930457, 2021 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-34489390

RESUMO

BACKGROUND We aimed to evaluate the value of prophylactic extended-field intensity-modulated radiation therapy (IMRT) in the treatment of locally advanced cervical cancer with multiple pelvic lymph node metastases (≥2) and negative common iliac and paraaortic lymph nodes. MATERIAL AND METHODS Thirty-four patient with newly diagnosed cervical cancer (IB1-IVA) and multiple pelvic lymph node metastases (≥2) confirmed by computed tomography and magnetic resonance imaging were randomly divided into an extended-field group (17 patients) and a pelvic-field group (17 patients). In the extended-field group, we added the drainage area of paraaortic lymph nodes on the pelvic field. The pelvic field was administered Dt 45.0 to 50.4 Gy, while the drainage area of paraaortic lymph nodes was administered Dt 40.0 to 45.0 Gy. Both groups were given Irl92 intracavitary radiotherapy after 3 weeks of external irradiation. The total dose of point A was 25.0 to 30.0 Gy, fractional 6.0 to 7.0 Gy. All patients had concurrent platinum-based chemotherapy once weekly until the end of radiotherapy. RESULTS No paraaortic lymph node metastasis was found in the extended-field group (P=0.0184), and disease-free survival (DFS) was prolonged (P=0.0286). Adverse effects in patients with III-IV degree myelosuppression were increased in the extended-field group (P=0.0324). However, all patients recovered after symptomatic treatment. CONCLUSIONS Prophylactic extended-field IMRT with chemotherapy reduced the metastasis rate of paraaortic lymph nodes and prolonged the DFS in patients with locally advanced cervical cancer and multiple pelvic lymph node metastases (≥2), while the toxic adverse effects were tolerated.

7.
J Colloid Interface Sci ; 607(Pt 1): 61-67, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34492354

RESUMO

Rechargeable alkaline nickel-zinc (Ni-Zn) batteries are attracting increased attention owing to their exceptional inherent safety and high specific capacity. Unfortunately, the limited power and cycling performances of these Ni-Zn batteries are still challenging. Herein, bimetal nickel-cobalt sulfide/ reduced graphene oxide (NiCo-S/RGO) composites with tunable compositions are synthesized by rational designing precursor and subsequent sulfidation treatment. NiCo-S is evenly anchored on RGO surface, resulting in increased number of electrochemical active sites, accelerated electrolyte ion diffusion, and enhanced electrical conductivity. Particularly, by tuning the Ni and Co composition ratios in NiCo-S, NiCo-S/RGO with a Ni to Co ratio of 2:1 (NiCo-S-2/RGO) shows a specific capacity of 145.7 mA h g-1 at 1 A g-1 and long-life cycling retention of 84.7% after 1000 cycles, and the above performances are superior than the controlled samples with other Ni to Co ratios. Furthermore, the as-assembled alkaline zinc batteries of NiCo-S-2/RGO//Zn deliver an impressive specific energy of 333.2 W h kg-1, showing great potential in practical applications. This experiment hopefully provides new idea for construction of high-performance electrodes of aqueous rechargeable batteries.

8.
J Plant Physiol ; 266: 153510, 2021 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-34521019

RESUMO

Source sink balance is one of the major determinants of carbon partitioning in plants. However, its effects on photosynthesis in fruit trees are largely unknown. In this work, the effects of low sink demand on net photosynthetic rate (Pn) and chlorophyll fluorescence after fruit removal (-fruit) in peach (Prunus persica (L.) Batsch cv. 'Zaojiubao') trees were investigated. The stepwise energy flow through photosystem II (PSII) at the reaction center (RC) was analyzed with quantitative analyses of fluorescence transient, also called JIP-test. We found that Pn was significantly lower and closely correlated to the leaf stomatal conductance (Gs) of -fruit trees than that of fruit retained (+fruit) trees. Leaf temperature (Tleaf) of -fruit trees was remarkably higher than that of +fruit trees. Day-time-period assays of chlorophyll (Chl) fluorescence revealed that, in the leaves of -fruit trees, the fluorescence parameters, such as NPQ (non-photochemical quenching coefficient) and ΦD0 (maximum quantum yield of non-photochemical de-excitation), decreased in the morning and recovered to the normal level in the afternoon, whereas other parameters, such as ΦE0 (quantum yield for electron transport at t = 0), Ψ0 (probability that a trapped exciton moves an electron to QA pool), F0 (minimum fluorescence, when all PSII RCs are open) and Wk (relative variable fluorescence at 300 µs of the chlorophyll fluorescence transient), did not. These results suggest that OEC complex and QA pool were irreversibly affected by low sink demand, whereas light harvest antenna and PSII potential efficiency retained a strong ability to recover.

9.
Cell Rep ; 36(11): 109690, 2021 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-34525358

RESUMO

Recombinant bacterial colonization plays an indispensable role in disease prevention, alleviation, and treatment. Successful application mainly depends on whether bacteria can efficiently spatiotemporally colonize the host gut. However, a primary limitation of existing methods is the lack of precise spatiotemporal regulation, resulting in uncontrolled methods that are less effective. Herein, we design upconversion microgels (UCMs) to convert near-infrared light (NIR) into blue light to activate recombinant light-responsive bacteria (Lresb) in vivo, where autocrine "functional cellular glues" made of adhesive proteins assist Lresb inefficiently colonizing the gut. The programmable engineering platform is further developed for the controlled and effective colonization of Escherichia coli Nissle 1917 (EcN) in the gut. The colonizing bacteria effectively alleviate DSS-induced colitis in mice. We anticipate that this approach could facilitate the clinical application of engineered microbial therapeutics to accurately and effectively regulate host health.

10.
Am J Clin Nutr ; 2021 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-34477820

RESUMO

BACKGROUND: Lipid metabolism plays an important role in the pathogenesis of diabetes. There is little evidence regarding the prospective association of the maternal lipidome with gestational diabetes mellitus (GDM), especially in Chinese populations. OBJECTIVES: We aimed to identify novel lipid species associated with GDM risk in Chinese women, and assess the incremental predictive capacity of the lipids for GDM. METHODS: We conducted a nested case-control study using the Tongji-Shuangliu Birth Cohort with 336 GDM cases and 672 controls, 1:2 matched on age and week of gestation. Maternal blood samples were collected at 6-15 wk, and lipidomes were profiled by targeted ultra-HPLC-tandem MS. GDM was diagnosed by oral-glucose-tolerance test at 24-28 wk. The least absolute shrinkage and selection operator is a regression analysis method that was used to select novel biomarkers. Multivariable conditional logistic regression was used to estimate the associations. RESULTS: Of 366 detected lipids, 10 were selected and found to be significantly associated with GDM independently of confounders: there were positive associations with phosphatidylinositol 40:6, alkylphosphatidylcholine 36:1, phosphatidylethanolamine plasmalogen 38:6, diacylglyceride 18:0/18:1, and alkylphosphatidylethanolamine 40:5 (adjusted ORs per 1 log-SD increment range: 1.34-2.86), whereas there were inverse associations with sphingomyelin 34:1, dihexosyl ceramide 24:0, mono hexosyl ceramide 18:0, dihexosyl ceramide 24:1, and phosphatidylcholine 40:7 (adjusted ORs range: 0.48-0.68). Addition of these novel lipids to the classical GDM prediction model resulted in a significant improvement in the C-statistic (discriminatory power of the model) to 0.801 (95% CI: 0.772, 0.829). For every 1-point increase in the lipid risk score of the 10 lipids, the OR of GDM was 1.66 (95% CI: 1.50, 1.85). Mediation analysis suggested the associations between specific lipid species and GDM were partially explained by glycemic and insulin-related indicators. CONCLUSIONS: Specific plasma lipid biomarkers in early pregnancy were associated with GDM in Chinese women, and significantly improved the prediction for GDM.

11.
Artigo em Inglês | MEDLINE | ID: mdl-34444598

RESUMO

This preliminary study examined the effects of a stretching intervention after training and its duration (15 vs. 30 min) on participants' shank circumference (SC) reduction and subjective discomfort score. Ten male volleyball players underwent a routine 3 h training. A two-way analysis of variance revealed that the stretching intervention had significant effects on SC reduction (p < 0.01) and subjective discomfort scores (p < 0.001). Stretching after training could help eliminate shank strain, and a slighter discomfort in shanks when stretching was also seen (score, 20.1/100). An independent-samples t test revealed a significantly higher SC reduction (p < 0.01) with 30 min of stretching (5.6 mm) than with 15 min of stretching (2.7 mm); both stretching durations reduced SC significantly more than the no-stretching condition did. The findings of this study can serve as a reference for volleyball players to alleviate shank strain after daily routine training.


Assuntos
Voleibol , Humanos , Perna (Membro) , Masculino
12.
Front Immunol ; 12: 690783, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34335595

RESUMO

Type 1 diabetes (T1D) is characterized by the unresolved autoimmune inflammation and islet ß cell destruction. The islet resident antigen-presenting cells (APCs) including dendritic cells and macrophages uptake and process the ß cell-derived antigens to prime the autoreactive diabetogenic T cells. Upon activation, those autoreactive T cells produce copious amount of IFN-γ, TNF-α and IL-1ß to induce ß cell stress and death. Autoimmune attack and ß cell damage intertwine together to push forward this self-destructive program, leading to T1D onset. However, ß cells are far beyond a passive participant during the course of T1D development. Herein in this review, we summarized how ß cells are actively involved in the initiation of autoimmune responses in T1D setting. Specifically, ß cells produce modified neoantigens under stressed condition, which is coupled with upregulated expression of MHC I/II and co-stimulatory molecules as well as other immune modules, that are essential properties normally exhibited by the professional APCs. At the cellular level, this subset of APC-like ß cells dynamically interacts with plasmacytoid dendritic cells (pDCs) and manifests potency to activate autoreactive CD4 and CD8 T cells, by which ß cells initiate early autoimmune responses predisposing to T1D development. Overall, the antigen-presenting function of ß cells helps to explain the tissue specificity of T1D and highlights the active roles of structural cells played in the pathogenesis of various immune related disorders.

13.
Artigo em Inglês | MEDLINE | ID: mdl-34341834

RESUMO

BACKGROUND: Increasing evidence has shown that the occurrence and development of various human diseases are closely related to the gut microbiota. We compared the gut microbial communities of human subjects with congenital insensitivity to pain with anhidrosis (CIPA) and healthy controls (HCs) to assess whether fecal microbiota transplantation (FMT) into germ-free mice and mice in acute pain influenced the behaviors of the host. METHODS: We utilized 16 s rRNA analysis to compare the gut microbial communities of CIPA subjects and HCs and assessed whether FMT into germ-free mice and mice in acute pain influenced the behaviors of the host. RESULTS: In a 16 s RNA analysis, the CIPA group had significant decreases in the relative abundance of 11 bacteria, whereas 7 bacteria were significantly increased. In further animal experiments, the transplantation of fecal samples from CIPA patients to healthy mice significantly increased their scores on both the mechanical withdrawal test and the tail flick test; in an acute plantar incision model, scores were also significantly increased on the mechanical withdrawal test at 4 and 5 days after the operation. Moreover, pseudo-germ-free mice receiving fecal bacteria from patients with CIPA took significantly longer to escape and had a significantly longer path length on training days 1, 2, and 5 and also had fewer platform crossings and spent less time in the target quadrant in the probe trial. CONCLUSIONS: Our results suggest that the gut microbiota in CIPA subjects plays a key role in behaviors. Therapeutic strategies for improving the gut microbiota might alleviate CIPA symptoms.

14.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 29(4): 1119-1122, 2021 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-34362490

RESUMO

OBJECTIVE: To investigate the auxiliary diagnostic value of serum adenosine deaminase (ADA) in acute leukemia (AL) at clinical test. METHODS: 123 AL patients hospitalized in Zhejiang hospital from November 2018 to March 2020 were enrolled as the observation group, and 98 healthy people in the same period were randomly enrolled as the control group. AL patients were divided into two groups: 77 acute myeloid leukemia (AML) patients for AML group and 46 acute lymphoblastic leukemia (ALL) patients for ALL group. The levels of adenosine deaminase (ADA), alanine aminotransferase (ALT), aspartate aminotransferase (AST), glutamyl transpeptidase (GGT), lactate dehydrogenase (LDH) and homocysteine (Hcy) in serum of the patients were detected, and the correlation of ADA with these items was analyzed. Receiver operating characteristic curve (ROC) was used to analyze the clinical diagnostic value of ADA, Yoden index was used to confirm the best cut-off point. RESULTS: The serum ADA level in AL patients was significant higher than that in control group (P < 0.05). The results of Pearson correlation analysis showed that there was a significant positive correlation of ADA with Hcy, ALT, AST, GGT, LDH in AML group (r = 0.47, r = 0.28, r = 0.37, r = 0.22, r = 0.55); and also there was a significant positive correlation of ADA with GGT in ALL group (r = 0.54). In AML group, the maximum area under ROC curve was 0.761 (P = 0.00), 95% confidence interval was 0.682-0.841, sensitivity was 54.50%, specificity was 98.90%, and the best cut-off point was 17.1 U/L. In ALL group, the maximum area under ROC curve was 0.785, 95% confidence interval was 0.694-0.877, sensitivity was 65.90%, specificity was 84.00%, and the best cut-off point was 13.45 U/L. CONCLUSION: The detection of ADA in serum can be used as an auxiliary examination in patients with AL, which can provide a certain value for the diagnosis of the disease.


Assuntos
Adenosina Desaminase , Leucemia Mieloide Aguda , Humanos , L-Lactato Desidrogenase , Leucemia Mieloide Aguda/diagnóstico , Curva ROC , Estudos Retrospectivos
15.
Nanomaterials (Basel) ; 11(7)2021 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-34361250

RESUMO

Supercapacitors (SCs) have been regarded as alternative electrochemical energy storage devices; however, optimizing the electrode materials to further enhance their specific energy and retain their rate capability is highly essential. Herein, the influence of nitrogen content and structural characteristics (i.e., porous and non-porous) of the NiS/nitrogen-doped carbon nanocomposites on their electrochemical performances in an alkaline electrolyte is explored. Due to their distinctive surface and the structural features of the porous carbon (A-PVP-NC), the as-synthesized NiS/A-PVP-NC nanocomposites not only reveal a high wettability with 6 M KOH electrolyte and less polarization but also exhibit remarkable rate capability (101 C/g at 1 A/g and 74 C/g at 10 A/g). Although non-porous carbon (PI-NC) possesses more nitrogen content than the A-PVP-NC, the specific capacity output from the latter at 10 A/g is 3.7 times higher than that of the NiS/PI-NC. Consequently, our findings suggest that the surface nature and porous architectures that exist in carbon materials would be significant factors affecting the electrochemical behavior of electrode materials compared to nitrogen content.

16.
Small ; : e2102349, 2021 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-34390180

RESUMO

Sodium-ion batteries (SIBs) have aroused wide concern due to their potential applications in large-scale energy-storage systems. In this work, a hybrid of Fe7 S8 nanoparticles/N-doped carbon nanofibers (Fe7 S8 /N-CNFs) is designed and synthesized via electrospinning. As an anode for SIBs, Fe7 S8 /N-CNFs exhibit a high reversible capacity of 649.9 mAh g-1 at 0.2 A g-1 after 100 cycles, and superior cycling stability for 2000 cycles at 1 A g-1 with only 0.00302% capacity decay per cycle. Such excellent performance originates from: i) Fe7 S8 nanoparticles (average diameter of 17 nm), which shorten the Na+ diffusion distance; ii) the unique 3D N-CNFs, which enhance the conductivity, alleviate the self-agglomeration and large volume change of Fe7 S8 nanoparticles, and offer numerous active sites for Na+ adsorption and paths for electrolyte diffusion. The fascinating structure and superior electrochemical properties of Fe7 S8 /N-CNFs shed light on developing high-performance SIBs anode materials.

17.
Bioresour Technol ; 341: 125824, 2021 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-34450443

RESUMO

To recover resource from waste activated sludge (WAS) is of great significance. This study proposed a promising way, i.e., stepwise alkaline treatment coupled with ammonia stripping, to remarkably enhance short-chain fatty acids (SCFAs) production from WAS anaerobic digestion. The maximal production of SCFAs, with the value of 323 mg COD/g volatile suspended solid, was obtained with first initial pH = 10 adjustment followed by second initial pH = 10 adjustment on third day coupled with ammonia stripping. Mechanistic studies showed that solubilization of both extracellular polymeric substances and cells could be accelerated by stepwise initial pH = 10 adjustment. However, without ammonia stripping, the activities of either acidogens or methanogens could be inhibited by free ammonia formed under alkaline conditions; positively, anaerobes related to SCFAs production were enriched with ammonia stripping. Moreover, the proposed strategy can simultaneously achieve nitrogen and carbon recovery, providing some solutions for the carbon-neutral operation of wastewater treatment plants.

18.
Anal Chem ; 93(33): 11634-11640, 2021 08 24.
Artigo em Inglês | MEDLINE | ID: mdl-34378382

RESUMO

Exploring the ratiometric fluorescence biosensing of DNA-templated biemissive silver nanoclusters (AgNCs) is significant in bioanalysis, yet the design of a stimuli-responsive DNA device is a challenge. Herein, using the anti-digoxin antibody (anti-Dig) with two identical binding sites as a model, a tweezer-like DNA architecture is assembled to populate fluorescent green- and red-AgNCs (g-AgNCs and r-AgNCs), aiming to produce a ratio signal via specific recognition of anti-Dig with two haptens (DigH). To this end, four DNA probes are programmed, including a reporter strand (RS) dually ended with a g-/r-AgNC template sequence, an enhancer strand (ES) tethering two same G-rich tails (G18), a capture strand (CS) labeled with DigH at two ends, and a help strand (HS). Initially, both g-AgNCs and r-AgNCs wrapped in the intact RS are nonfluorescent, whereas the base pairing between RS, ES, CS, and HS resulted in the construction of DNA mechanical tweezers with two symmetric arms hinged by a rigid "fulcrum", in which g-AgNCs are lighted up due to G18 proximity ("green-on"), and r-AgNCs away from G18 are still dark ("red-off"). When two DigHs in proximity recognize and bind anti-Dig, the conformation switch of these tweezers resultantly occurs, taking g-AgNCs away from G18 for "green-off" and bringing r-AgNCs close to G18 for "red-on". As such, the ratiometric fluorescence of r-AgNCs versus g-AgNCs is generated in response to anti-Dig, achieving reliable quantization with a limit of detection at the picomolar level. Based on the fast stimulated switch of unique DNA tweezers, our ratiometric strategy of dual-emitting AgNCs would provide a new avenue for a variety of bioassays.


Assuntos
Técnicas Biossensoriais , Nanopartículas Metálicas , Anticorpos , DNA , Fluorescência , Prata , Espectrometria de Fluorescência
19.
Molecules ; 26(15)2021 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-34361584

RESUMO

In this study, a series of N-heterocyclic indolyl ligand precursors 2-Py-Py-IndH, 2-Py-Pz-IndH, 2-Py-7-Py-IndH, 2-Py-7-Pz-IndH, and 2-Ox-7-Py-IndH (L1H-L5H) were prepared. The treatment of ligand precursors with 1 equivalent of palladium acetate affords palladium complexes 1-5. All ligand precursors and palladium complexes were characterized by NMR spectroscopy and elemental analysis. The molecular structures of complexes 3 and 5 were determined by single crystal X-ray diffraction techniques. The application of those palladium complexes 1-5 to the Suzuki reaction with aryl halide substrates was examined.

20.
Urol J ; 2021 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-34431075

RESUMO

PURPOSE: To evaluate the ability of bacterial scatter diagrams generated from the automated urine particle analyzer (UF-1000i, Sysmex, Kobe, Japan) in pre-estimate the treatment efficacy of oral cefalexin in treating women with uncomplicated urinary tract infection (uUTI). MATERIALS AND METHODS: Over 3 years, women 20-80 years old with symptoms suggestive of uUTI (Urinary Tract Infection Symptoms Assessment symptom score, ,UTISA > 3) and bacteriuria (bacterial count ≥100/uL) were enrolled. After informed consent, patients took cephalexin 500mg 4 times/day for 7 days. The voided urine specimens were classified into rods or cocci/mixed group automatically through the built-in software of the UF1000i. Patients were followed up with UTISA on the 3rd day after treatment and returned to the clinic on the 7th day and followed for additional UTISA and urine analysis. Symptom and laboratory improvement were defined as UTISA <4 and bacterial count <100/uL, respectively, on the 7th day. RESULTS: Of 99 women (age: 49.91 ±15.32 years) eligible for analysis, 80 were classified as having urine that contained rods and 19 as cocci/mixed. Symptom improvement was observed in 62 women in the rods group and 11 women in the cocci/mixed group (p=0.08). Laboratory improvement was noted in 64 women in the rods group and 10 women in the cocci/mixed group (p=0.01). On day 7, treatment success with both symptom and laboratory improvement was more observed in rods than in cocci/mixed group (61.3% vs. 26.3%, p<0.01). CONCLUSION: The automatic urine particle analyzer can pre-estimate the treatment response of antibiotics in women with uUTI.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...