Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 817
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Exp Biol Med (Maywood) ; : 1535370220911604, 2020 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-32148090
2.
Inorg Chem ; 2020 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-32162905

RESUMO

Photodynamic therapy (PDT) is commonly employed in clinics to treat the cancer, but because of the hypoxic tumor microenvironment prevalent inside tumors, PDT therapeutic efficiency is not adequate hence limiting the effectiveness of PDT. Therefore, we designed a nanocomposite consisting of reduced nanographene oxide (rGO) modified with polyethylene glycol (PEG), manganese dioxide (MnO2), upconversion nanoparticles (UCNPs), and Chlorin e6 (Ce6) to spark oxygen production from H2O2 with the aim of relieving the tumor hypoxic microenvironments. For in vivo tumor PDT and photothermal therapy (PTT), UCNPs-Ce6-labeled rGO-MnO2-PEG nanocomposites were used as a therapeutic agent, augmenting the therapeutic efficiency of PDT via redox progression through the catalytic H2O2 decomposition pathway and further achieving excellent tumor inhibition. It is important to mention that degradation of MnO2 in an acidic cellular microenvironment leads to the creation of a massive volume of Mn2+ which was employed as a contrast mediator for magnetic resonance imaging (MRI). Our research postulates an approach to spark O2 formation through an internal stimulus to augment the efficiency of MRI- and computerized tomography (CT)-imaging-guided PDT and PTT.

3.
Artigo em Inglês | MEDLINE | ID: mdl-32117795

RESUMO

Temperate phages are considered as natural vectors for gene transmission among bacteria due to the ability to integrate their genomes into a host chromosome, therefore, affect the fitness and phenotype of host bacteria. Many virulence genes of pathogenic bacteria were identified in temperate phage genomes, supporting the concept that temperate phages play important roles in increasing the bacterial pathogenicity through delivery of the virulence genes. However, little is known about the roles of temperate phages in attenuation of bacterial virulence. Here, we report a novel Bordetella bronchiseptica temperate phage, vB_BbrS_PHB09 (PHB09), which has a 42,129-bp dsDNA genome with a G+C content of 62.8%. Phylogenetic analysis based on large terminase subunit indicated that phage PHB09 represented a new member of the family Siphoviridae. The genome of PHB09 contains genes encoding lysogen-associated proteins, including integrase and cI protein. The integration site of PHB09 is specifically located within a pilin gene of B. bronchiseptica. Importantly, we found that the integration of phage PHB09 significantly decreased the virulence of parental strain B. bronchiseptica Bb01 in mice, most likely through disruption the expression of pilin gene. Moreover, a single shot of the prophage bearing B. bronchiseptica strain completely protected mice against lethal challenge with wild-type virulent B. bronchiseptica, indicating the vaccine potential of lysogenized strain. Our findings not only indicate the complicated roles of temperate phages in bacterial virulence other than simple delivery of virulent genes but also provide a potential strategy for developing bacterial vaccines.

4.
Artigo em Inglês | MEDLINE | ID: mdl-32204427

RESUMO

Coastal wetlands provide many critical ecosystem services including carbon storage. Soil organic carbon (SOC) is the most important component of carbon stock in coastal salt marshes. However, there are large uncertainties when estimating SOC stock in coastal salt marshes at large spatial scales. So far, information on the spatial heterogeneity of SOC distribution and determinants remains limited. Moreover, the role of complex ecological interactions in shaping SOC distribution is poorly understood. Here, we report detailed field surveys on plant, soil and crab burrowing activities in two inter-tidal salt marsh sites with similar habitat conditions in Eastern China. Our between-site comparison revealed slight differences in SOC storage and a similar vertical SOC distribution pattern across soil depths of 0-60 cm. Between the two study sites, we found substantially different effects of biotic and abiotic factors on SOC distribution. Complex interactions involving indirect effects between soil, plants and macrobenthos (crabs) may influence SOC distribution at a landscape scale. Marked differences in the SOC determinants between the study sites indicate that the underlying driving mechanisms of SOC distribution are strongly system-specific. Future work taking into account complex interactions and spatial heterogeneity is needed for better estimating of blue carbon stock and dynamics.

5.
Endocrine ; 2020 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-32172485

RESUMO

PURPOSE: To identify the volume changes of hippocampus subfields in T2DM patients with cognitive impairment and to determine how these atrophy patterns associate with impairments in different cognitive domain. METHODS: A total of 117 individuals were recruited, including T2DM patients with cognitive impairment (T2DM-CI) (n = 34), T2DM patients without cognitive impairment (T2DM-non-CI) (n = 36) and normal controls (NC) (n = 47). All subjects went through a 3.0 T magnetic resonance (MR) scan and a neuropsychological assessment. Hippocampal subfield volumes were processed using the FreeSurfer 6.0.0 and compared among the three groups. Partial correlation analyses were used to estimate the relationship between cognitive function and hippocampal subfield volume, with age, sex, education, and eTIV (estimated total intracranial volume) as covariants. RESULTS: The total hippocampal volume had a reduction trend among the three groups, and the significantly statistical difference only was found between T2DM-CI group and NC group. Regarding the hippocampal subfields, the volumes of left subiculum, left presubiculum, left fimbria, right CA1 and right molecular layer HP decreased significantly in the T2DM-CI group (P < 0.05/12). Partial correlation analyses showed that the volumes of the left subiculum, left fimbria, and left presubiculum were significantly related to executive function. The right hippocampal CA1 volume was significantly correlated with memory in the T2DM-CI group (P < 0.05). But in T2DM-non-CI group, the correlation between the left fimbria volume and the memory, the left subiculum volume and MoCA were different with the T2DM-CI group and NC group (P < 0.05). CONCLUSIONS: The smaller the volume of left presubiculum, the worse the executive function, and the atrophy of the right CA1 was related to memory impairment in T2DM-CI group. However the result was the opposite in T2DM-non-CI group. There might be a compensation mechanism of hippocampus of T2DM patients before cognitive impairment.

6.
Environ Int ; 138: 105620, 2020 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-32179315

RESUMO

BACKGROUNDS: Previous studies linking biomass fuel use to hypertension have been inconsistent. We investigated the association between biomass fuel use and the risk of hypertension and blood pressure measures in older Chinese people. METHODS: The prospective cohort study of the Chinese Longitudinal Healthy Longevity Survey (CLHLS) included participants aged 65 years and older in 2011/2012 who were followed up until 2014 in 23 provinces in China. We explored the association between biomass fuel use and hypertension using the Cox proportional hazards model and examined the relationship of biomass fuel use with blood pressure measures using the generalized estimating equation. Additionally, we examined the effect of switching cooking fuels on hypertension during the follow-up. RESULTS: Among 3754 participants who were without hypertension at baseline, the mean age was 86 years old, and 47.5% of participants were men. Reported use of biomass fuel for cooking (50.2%) was associated with a higher risk of hypertension (incidence rate (IR) per 100 person-years: 13.15 versus 12.99, hazard ratio (HR) = 1.15, 95% confidence interval (CI) = 1.01-1.31). Biomass fuel use was related to systolic blood pressure (SBP) (ß 1.10 mmHg, 95% CI: 0.48-1.72), diastolic blood pressure (DBP) (ß 1.02 mmHg, 95% CI: 0.61-1.43) and mean arterial pressure (MAP) (ß 1.03 mmHg, 95% CI: 0.63-1.43) elevation. Compared with persistent clean fuel users, participants who reported switching from clean to biomass fuels for cooking had a noticeably higher risk of hypertension (IR per 100 person-years: 14.27 versus 12.81, HR 1.49, 95% CI: 1.16-1.90) and higher SBP (3.71 mmHg), DBP (2.44 mmHg) and MAP (2.86 mmHg). Interaction and stratified analyses showed greater effect estimates of SBP and MAP in the oldest oldpeople (≥85). CONCLUSIONS: The use of biomass fuel for cooking was associated with greater hypertension risk, and the risk may be higher among those who switched from clean fuels to biomass fuels in the Chinese elderly population. Biomass fuel use was associated with a statistically significant but small absolute increase in blood pressure measures.

7.
Int J Pharm ; 580: 119240, 2020 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-32197983

RESUMO

Multiple carriers may be used to prepare solid dispersions (SDs) for different purposes. The aim of this work is to investigate the effect of the third component on the physical stability and physical aging behavior of cinnarizine-soluplus SDs. HPMC, PVP, sorbitol and citric acid were used as the third component to prepare cinnarizine ternary SDs using hot melt extrusion method. The resultant samples were stored at 25 °C or under stress conditions. Differential scanning calorimetry, powder X-ray diffraction and dissolution tests were performed to investigate the changes of samples during the storage. Infrared spectroscopy was used to evaluate the interactions between drug and carriers. Results showed that the addition of HPMC or PVP enhanced the physical stability of ternary SDs stored at 25 °C rather than those stored under stress conditions. Sorbitol did not show any improvements in physical stability of samples stored at 25 °C or under stress conditions. Surprisingly, the physical stability of samples stored at 25 °C or under stress conditions was enhanced significantly by citric acid due to the ionic and hydrogen bonding interactions. The miscibility between drug and carriers as well as between different carriers should be considered when using multiple carriers. The third component can act as a "linker" by interacting with drug and polymer to enhance the physical stability of SDs effectively.

8.
Medicine (Baltimore) ; 99(11): e19489, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32176087

RESUMO

RATIONALE: Fibrous hamartoma of infancy (FHI) is a rare benign soft-tissue tumor in children with a triphasic organoid histologic appearance. PATIENT CONCERNS: We here report a case with the largest FHI published so far. A 13-month-old boy with a rapidly growing tumor 45 cm in length and 69.3 cm in circumference of the left lower extremity was identified immediately at birth. DIAGNOSES: The diagnosis was uncertain before the operation, although biopsy was conducted. The postoperation histological examination showed arranged spindle cells, adipose tissue, and nests of immature small cells embedded in a myxoid background, which is the characteristic triphasic histology of FHI. INTERVENTIONS: Under general anesthesia, hip and lower extremity amputation was performed. OUTCOMES: The patient was followed-up for 6 years and no signs of recurrence were found, suggesting a favorable prognosis, although a part of the residual tumor was present after the surgery. LESSONS: FHI exhibits pathological and clinical characteristics. However, differential diagnosis of FHI from other soft-tissue tumors before operation remains a challenge. Thus, sometimes, aggressive therapy for the treatment of FHI might be a good choice to get a satisfactory.


Assuntos
Hamartoma/diagnóstico por imagem , Perna (Membro) , Neoplasias de Tecidos Moles/diagnóstico por imagem , Tecido Adiposo/patologia , Amputação , Angiografia por Tomografia Computadorizada , Diagnóstico Diferencial , Hamartoma/patologia , Hamartoma/cirurgia , Humanos , Lactente , Imagem por Ressonância Magnética , Masculino , Neoplasias de Tecidos Moles/patologia , Neoplasias de Tecidos Moles/cirurgia
9.
Med Sci Monit ; 26: e921692, 2020 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-32160184

RESUMO

BACKGROUND Many patients are not responsive or tolerant to medical therapies for carotid atherosclerosis. Thus, elucidating the molecular mechanism for the pathogenesis and progression of carotid atherosclerosis and identifying new potential molecular targets for medical therapies that can slow progression of carotid atherosclerosis and prevent ischemic events are quite important. MATERIAL AND METHODS We downloaded the expression profiling data of PBMC in Biobank of Karolinska Endarterectomy (BiKE, GSE21545) for GEO. The WGCNA and DEG screening were conducted. The co-expression pattern between patients with ischemic events (the events group) and patients without ischemic events (the no-events group) were compared. Then, we identified hub genes of each module. Finally, the DEG co-expression network was constructed and MCODE was used to identify crucial genes based on this co-expression network. RESULTS In the study, 183 DEGs were screened and 8 and 6 modules were assessed in the events group and no-events group, respectively. Compared to the no-events group, genes associated with inflammation and immune response were clustered in the green-yellow module of the events group. The hub gene of the green-yellow module of the events group was KIR2DL5A. We obtained 1 DEG co-expression network, which has 16 nodes and 24 edges, and we detected 5 crucial genes: SIRT1, THRAP3, RBM43, PEX1, and KLHDC2. The upregulated genes (THRAP3 and RBM43) showed potential diagnostic and prognostic value for the occurrence of ischemic events. CONCLUSIONS We detected 8 modules for the events group and 6 modules for the no-events group. The hub genes for modules and crucial genes of the DEG co-expression network were also identified. These genes might serve as potential targets for medical therapies and biomarkers for diagnosis and prognosis. Further experimental and biological studies are needed to elucidate the role of these crucial genes in the progression of carotid atherosclerosis.

10.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 28(1): 40-50, 2020 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-32027251

RESUMO

OBJECTIVE: To investigate the correlation of single nucleotide polymorphisms (SNP) in arachidonate 5-lipoxygenase gene (ALOX5) rs2029253, rs2228064 and rs2228065 sites, 5-lipoxygenase activating protein gene (ALOX5AP) rs10507391, rs4769874 sites with the risk for genesis of adult myeloid leukemia. METHODS: By the approval from the hospital ethics committee and the informed consent of participants. 150 patients with myeloid leukemia (ML) as ML group and 134 healthy people as the control group were selected. The genomic DNA was extracted from the samples. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) combined with directly sequencing, PCR-amplified products were applied to test the polymorphism of 5 sites in ALOX5 and ALOX5AP gene. RESULTS: A allele frequencies of ALOX5 gene rs2029253 site in the ML group and the control group were 43.0% and 34.3%, respectively. And the G allele frequencies in the ML group and the control group were 57.0% and 65.7%, respectively. The genotype distributions of AA, AG and GG in ALOX5 gene rs2029253 site in the ML group were 32.2%, 21.5% and 46.3% respectively. That in the control group were 15.7%, 37.3% and 47.0% respectively. The genotype AA and A allele frequency of ALOX5 gene rs2029253 site were linked with the increased risk of myeloid leukemia (OR=2.26, 95% CI: 1.43-4.56, P<0.05; OR=1.44, 95% CI: 1.02-2.03, P<0.05). And the genotype AG and allele G reduced the susceptibility to myeloid leukemia (OR=0.46, 95% CI: 0.27-0.78, P<0.01; OR=0.69, 95% CI: 0.50-0.98, P<0.05), however, the polymorphisms of ALOX5 gene rs2228064 and rs2228065 site not correlated with the risk of myeloid leukemia (P>0.05). The A allele frequency of ALOX5AP gene rs10507391 site in the ML group and the control group were 30.7% and 36.2% respectirely. The genotype distribution rates of AA, AT and TT in ALOX5AP gene rs10507391 site in the ML group was 1.3%, 58.7% and 40.0% respectively, that in the control group were 9.7%, 53.0% and 37.3% respectively. The genotype AA of ALOX5AP gene rs10507391 site correlated with the decreased risk of myeloid leukemia (OR=0.13, 95% CI: 0.03-0.57, P<0.05), but the polymorphism of ALOX5AP gene rs4769874 site not correlated with the risk of myeloid leukemia (P>0.05). CONCLUSION: The genotype AA, AG and allele A, G of ALOX5 rs2029253, as well as ALOX5AP rs10507391 may be correlate with the susceptibility to myeloid leukemia.


Assuntos
Proteínas Ativadoras de 5-Lipoxigenase/genética , Araquidonato 5-Lipoxigenase/genética , Leucemia Mieloide , Adulto , Estudos de Casos e Controles , Frequência do Gene , Predisposição Genética para Doença , Genótipo , Humanos , Leucemia Mieloide/genética , Polimorfismo de Nucleotídeo Único , Fatores de Risco
11.
Thorac Cancer ; 11(3): 754-761, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32012484

RESUMO

BACKGROUND: There is no consensus on the definition or recommended radiotherapy treatment of ultracentral non-small cell lung cancer (NSCLC). Here, we report our institution's experience in treating ultracentral lung cancer patients with stereotactic ablative radiotherapy (SABR) of 60 Gy in eight fractions. METHODS: We retrospectively reviewed the outcomes of 21 ultracentral NSCLC patients treated with 60 Gy SABR in eight fractions. We defined ultracentral lung cancer as the planning target volume (PTV) directly abutting or overlapping central structures, including the proximal bronchial tree, heart, and great vessels but not the esophagus. The Kaplan-Meier method was used to estimate overall survival (OS), progression-free survival (PFS) and local control (LC). Toxicity was scored per the CTCAE v4.03. RESULTS: The median follow-up time was 15 months, and the median OS was 15 months. The one- and two-year OS rates were 87.5% and 76.6%, respectively. The one- and two-year PFS rates were 71.1% and 64.0%, respectively. The one- and two-year LC rates were 92.9% and 92.9%, respectively. The rate of grade 2 treatment-related toxicities was 19.1%. There was no grade ≥ 3 treatment-related toxicity. CONCLUSION: SABR of 60 Gy in eight fractions is feasible for ultracentral NSCLC.

12.
Int J Biol Macromol ; 149: 901-907, 2020 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-32007550

RESUMO

Black soldier fly can convert organic wastes into their own functional biological macromolecules i.e. chitin that has great potential for biotechnological, biomedical and cosmetic application. The variation in the physiochemical structure of chitin was proved for several insects during metamorphosis stages whereas, it remains unknown for black soldier fly (BSF), a recognized resource insect for industrial production and organic waste management. The current work noted results on how the chitin matrix can undergo physicochemical changes during the developmental phases (larvae, prepupa, puparium, and adults) of BSF. Chitin content was determined around 3.6%, 3.1%, 14.1% and 2.9%, respectively. Fourier transform infrared spectroscopy, thermogravimetric analysis and x-ray diffraction analysis showed that chitin from BSF different stages was all α-chitin with similar thermal stability. The chitin crystalline index increased gradually with development from larvae to adult, 33.09%, 35.14%, 68.44% and 87.92%, respectively. Moreover, it was observed by scan electron microscopy that surface morphology characteristics of chitin vary significantly in developmental phases. These results confirmed that Hermetia illucens is promising for converting organic wastes into valuable biopolymers i.e. chitin and its physiochemical properties in various developmental stages help to determine the related biomedical, biotechnological, cosmetic and functional food utilization potential.

13.
Mar Pollut Bull ; 151: 110800, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32056595

RESUMO

Few investigations of iron speciation exist in intertidal conditions with little attention given to understanding the geochemical behavior of Fe3+-Fe2+ and its biogeochemical processes. Here, one sediment core was collected in Yunxiao mangrove forests, Zhangjiang estuary, China and iron mineralogy was determined by magnetic methods and Mössbauer spectroscopy. Changes in magnetic susceptibility, susceptibility of anhysteretic remanent magnetization, and saturation isothermal remanent magnetization were linked to changes of pseudo-single domain soft coercive components with minor antiferromagnetic fractions and the presence of minimal concentrations of superparamagnetic particles was confirmed by the no frequency dependent of AC magnetization. A positive correlation between L-ratio and hard isothermal remanent magnetization is congruent with the distribution of high-coercivity antiferromagnetic minerals. At 295 K, the Mössbauer spectrum of specimens was well fitted with a single magnetic sextet, hematite and two magnetic doublets, paramagnetic Fe2+ and paramagnetic Fe3+. The usefulness of magnetic data and Mössbauer spectroscopy could offer valuable analytical tools for tracking changes of iron speciation and phase in the intertidal conditions.


Assuntos
Monitoramento Ambiental , Estuários , Ferro , Áreas Alagadas , China , Minerais
14.
Curr Med Imaging Rev ; 16(2): 156-163, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32003316

RESUMO

BACKGROUND: Image reconstruction of magnetic induction tomography (MIT) is a typical ill-posed inverse problem, which means that the measurements are always far from enough. Thus, MIT image reconstruction results using conventional algorithms such as linear back projection and Landweber often suffer from limitations such as low resolution and blurred edges. METHODS: In this paper, based on the recent finite rate of innovation (FRI) framework, a novel image reconstruction method with MIT system is presented. RESULTS: This is achieved through modeling and sampling the MIT signals in FRI framework, resulting in a few new measurements, namely, fourier coefficients. Because each new measurement contains all the pixel position and conductivity information of the dense phase medium, the illposed inverse problem can be improved, by rebuilding the MIT measurement equation with the measurement voltage and the new measurements. Finally, a sparsity-based signal reconstruction algorithm is presented to reconstruct the original MIT image signal, by solving this new measurement equation. CONCLUSION: Experiments show that the proposed method has better indicators such as image error and correlation coefficient. Therefore, it is a kind of MIT image reconstruction method with high accuracy.

15.
J Am Chem Soc ; 2020 Feb 24.
Artigo em Inglês | MEDLINE | ID: mdl-32048845

RESUMO

Unveiling the mystery of the contribution of nonsurface or noninterface sites in a catalyst to its catalytic performance remains a great challenge because of the difficulty in capturing precisely structural information (surface plus inner) encoded in the catalyst. This work attempts to elucidate the critical role of the internal vacancy in an atomically precise 24-atom gold cluster in regulating the catalytic performance on the hydrogenation reaction of CO2. The experiment results show that the Au24 cluster with internal vacancy can mitigate sintering and exhibit high catalytic activity under relatively harsh reaction conditions, in contrast to the structurally similar Au25 cluster without internal vacancy. Our computational study suggests that the internal vacancy in Au24 provides the cluster with much more structural flexibility, which may be crucial to resisting the aggregation of the cluster and further postponing the deactivation. The hydrogenation and coupling stages of the reaction intermediates are proposed to explain the potential reaction pathway of CO2 with H2 on the Au24 catalyst with internal vacancy.

17.
Theranostics ; 10(3): 1213-1229, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31938061

RESUMO

The merits of nanomedicines are significantly impacted by the surrounding biological environment. Similar to the protein corona generated on the surface of nanoparticles in the circulation system, the intracellular protein corona (IPC) might be formed on nanoparticles when transported inside the cells. However, little is known currently about the formation of IPC and its possible biological influence. Methods: Caco-2 cells, a classical epithelial cell line, were cultured in Transwell plates to form a monolayer. Gold nanoparticles (AuNPs) were prepared as the model nanomedicine due to their excellent stability. Here we focused on identifying IPC formed on the surface of AuNPs during cell transport. The nanoparticles in the basolateral side of the Caco-2 monolayer were collected and analyzed by multiple techniques to verify IPC formation. High-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics was utilized to analyze the composition of IPC proteins. In particular, we established a dual-filtration strategy to exclude various interference in IPC identification. Based on the subcellular localization of specific IPC proteins, we elicited the nano-trafficking network of AuNPs. The transport pathways of AuNPs identified by proteomic analysis were also verified by various conventional technologies. Finally, we explored the influence of IPC on the uptake and stress response of endothelium. Results: The existence of IPC was demonstrated on the surface of AuNPs, in which 227 proteins were identified. Among them, 40 proteins were finally ascertained as the specific IPC proteins. The subcellular location analysis indicated that these "specific" IPC proteins could back-track the transport pathways of nanoparticles in the epithelial cell monolayer. According to the subcellular distribution of IPC proteins and co-localization, we discovered a new pathway of nanoparticles from endosomes to secretory vesicles which was dominant during the transcytosis. After employing conventional imageology and pharmacology strategies to verify the result of proteomic analysis, we mapped a comprehensive intracellular transport network. Our study also revealed the merits of IPC analysis, which could readily elucidate the molecular mechanisms of transcytosis. Besides, the IPC proteins increased the uptake and stress response of endothelium, which was likely mediated by extracellular matrix and mitochondrion-related IPC proteins. Conclusion: The comprehensive proteomic analysis of IPC enabled tracing of transport pathways in epithelial cells as well as revealing the biological impact of nanoparticles on endothelium.

18.
Biomolecules ; 10(1)2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31906487

RESUMO

The phytochemical investigation of Fraxinus hupehensis led to the isolation and characterization of ten compounds which were identified as fraxin (1), fraxetin (2), esculetin (3), cichoriin (4), euphorbetin (5), kaempferol-3-O-ß-rutinoside (6), oleuropein (7), linoleic acid (8), methyl linoleate (9), and ß-sitosterol (10). Structures of the isolated constituents were characterized by 1H NMR, 13C NMR and HRMS. All the compounds, except compounds 3 and 4, were isolated for the first time from this plant. Further, this was the first report for the occurrence of compound 5 in the Fraxinus species. Antifungal activity evaluation showed that compound 2 exhibited significant inhibitory effects against Bipolaris maydis, Sclerotium rolfsii, and Alternaria solani with EC50 values of 0.31 ± 0.01 mmol/L, 10.50 ± 0.02 mmol/L, and 0.40 ± 0.02 mmol/L respectively, compared to the positive control, Carbendazim, with its EC50 values of 0.74 ± 0.01 mmol/L, 1.78 ± 0.01 mmol/L and 1.41 ± 0.00 mmol/L. Herbicidal activity tests showed that compounds 8-10 had strong inhibitory effects against the roots of Echinochloa crus-galli with EC50 values of 1.16 ± 0.23 mmol/L, 1.28 ± 0.58 mmol/L and 1.33 ± 0.35 mmol/L respectively, more potently active than that of the positive control, Cyanazine, with its EC50 values of 1.56 ± 0.44 mmol/L. However, none of the compounds proved to be active against the tested bacteria (Erwinia carotovora, Pseudomonas syringae, and Ralstonia solanacearum).

19.
Nanoscale ; 12(3): 2063-2070, 2020 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-31912846

RESUMO

Bridging S22- moieties have been demonstrated to be highly active sites existing in metal polysulfides for the hydrogen evolution reaction (HER), thus the incorporation of high-density bridging S22- into a Ni3S2 material to improve its electrocatalytic HER performance is highly desirable and challenging. Herein, we report a novel Ni3S2 nanorod array decorated with (020)-oriented VS4 nanocrystals grown on nickel foam (Shig-NS-rod/NF) via a simple and facile solvothermal method. Results show that the in situ incorporation of VS4 not only triggers the formation of such a nanorod array structure, but also contributes to the uniform grafting of high-density and high catalytically active bridging S22- sites on the interface between Ni3S2 and VS4 for enhanced HER activity, and also promotes the absorption ability of OH- radicals and thus accelerates the HER Volmer step in alkaline media. As expected, the resultant Shig-NS-rod/NF material exhibits impressive catalytic performance toward the HER, with a much lower overpotential of 137 mV at 10 mA cm-2 and a long-term durability for at least 22 h, and is superior to Ni3S2 nanorod arrays with low-density bridging S22- (Slow-NS-rod/NF) and NS-film/NF counterparts (without VS4), even outperforming the NF-supported 20% Pt/C at a large current density of over 120 mA cm-2. Our findings put forward fresh insight into the rational design of highly efficient electrocatalysts toward the HER for green hydrogen fuel production.

20.
Arch Virol ; 165(3): 785-788, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31980938

RESUMO

In this study, the complete genomic sequence of a novel botoulivirus (Sclerotinia minor botoulivirus 1, SmBV1) from the phytopathogenic fungus Sclerotinia minor strain LC45 was determined. The genome of SmBV1 is 2,882 nucleotides in length and contains a single large open reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRp). Phylogenetic analysis showed that SmBV1 clustered with the botoulivirus clade within the family Botourmiaviridae. This is the first report of a botoulivirus in S. minor.


Assuntos
Ascomicetos/virologia , Micovírus/isolamento & purificação , Micovírus/fisiologia , Sequência de Aminoácidos , Micovírus/genética , Filogenia , Proteínas Virais/genética , Proteínas Virais/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA